Soheir Mansy - Academia.edu (original) (raw)

Papers by Soheir Mansy

The present work dealswith the simultaneous ultrastructure and triple immunofluorescence study of... more The present work dealswith the simultaneous ultrastructure and triple immunofluorescence study of the
three main hepatic fibrogenic cells, hepatic stellate cell, myofibroblast (MF), and fibroblast, in a group of
hepatitis C virus (HCV) RNA positive patients, as their exact interrelation behavior in vivo with the
progress of hepatic fibrosis is still inadequate. In this study, for the first time, cells having the morphological
characteristic of MF and not bone marrow fibrocytes were revealed in liver portal vessels. This
necessitates the reevaluation of the available knowledge concerning bone marrow fibrocyte. Also, the
distribution, cellular interrelations, and the fate of MF were highlighted.

13 14 Aims: the objective of the current study was to formulate nystatin (Nyst) into nanostructur... more 13 14 Aims: the objective of the current study was to formulate nystatin (Nyst) into nanostructured lipid carriers (NLCs) to enhance its antifungal activity. Place and Duration of Study: Department of pharmaceutical technology, national research centre, Egypt, between mars 2011 to april 2013 Methodology: Nyst loaded NLCs (NYST-NLCs) were prepared by the hot homogenization and ultrasonication method followed by evaluation of its topical effect on the cutaneous candidiasis. The prepared Nyst-NLCs were characterized for entrapment efficiency, particle size, zeta potential, morphology (transmission electron microscopy), thermal characterisation (differential scanning calorimetry) and in vitro drug release. The study design involves the investigation of the effect of three independent variables namely liquid lipid type (Miglyol 812 and Squalene), liquid lipid concentration (30 and 50%) and Nyst concentration (0.125 and 0.25%). A stability study for 6 months was performed. A microbiological study was conducted in male rats infected with Candida albicans. Results: NLC dispersions were spherical in shape with particle size ranging from 68.06±6.56 to 141.8±3.33 nm. The entrapment efficiencies ranged from 45.50±2.34 to 92.73±0.33% with zeta potential ranging from-26.2 to-39.2 mV. The stability studies done for 6 months indicated that Nyst-NLCs were stable for more than 6 monthes.the microbiological studies showed a least number of colonies forming units (cfu/ml) were recorded for the selected Nyst-NLCs compared to the drug solution and the Nystatin® cream present in the market. Conclusion: It can be fulfilled from this work that NLCs represent promising carrier for topical delivery of Nyst offering good physical stability, high entrapment efficiency and controlled drug release. Nyst-NLCs are a good candidate for cutaneous treatment of fungal infection Note: Review paper may have different types of subsections. 15

Helicobactor pylori (H. pylori) has been strongly associated with gastritis, peptic ulcer and is ... more Helicobactor pylori (H. pylori) has been strongly associated with gastritis, peptic ulcer and is linked to an increased risk of gastric cancer. The cytotoxin-associated gene product (cagA) and the vacuolating cytotoxin (vacA) have been implicated as two major virulence factors of H. pylori. Since there is an increasing evidence that genetic variability of H. pylori may have clinical importance, we aimed to evaluate different vacA genotypes and reveal its relationship with endoscopic and transmission electron microscopy (TEM) findings among H. pylori infected Egyptian patients. Forty H. pylori infected patients possessing vacA gene who underwent upper endoscopic examination were considered to be infected with H. pylori when rapid urease test and detection of 16S rRNA in gastric biopsy recorded positive. Both vacA and cagA genotypes were detected by polymerase chain reaction (PCR). The TEM was performed to assess the ultra-structure of the gastric mucosa. Four vacA genotypes were identified, the most prominent was the s2/m2 allele combination (52.5%) followed by s1/m1 (27.5%), s1/m2 (17.5%) and s2/m1 genotype was found just in one H. pylori strain (2.5%). There were significant correlations between vacA s2/m2 and gastritis (65.2%), and vacA s1/m1 and peptic ulceration (57%). The cagA gene was associated with 38% of vacA genotypes and 60% of which were significantly associated with vacA s1/m1 genotype with the development of severe gastritis reaching up to gastric ulcer. The TEM revealed H. pylori spiral and coccoid forms, cytoplasmic vacuolar degeneration caused by vacA, swollen mitochondria and dilated rough endoplasmic reticulum. In Egypt where prevalence of H. pylori infection is high, genotyping of H. pylori virulence factors can help to predict patients who are at a high risk of related gastroduodenal diseases. Although H. pylori with vacA s2/m2 genotype is mostly related to low level of virulent strains yet, significant crosstalk between H. pylori strains harboring both vacA s1/m1 and cagA gene provides crucial insights into virulence of high level.

The prevalence of methicillin-resistant Staphyloccoccus aureus (MRSA) strains has presented a new... more The prevalence of methicillin-resistant Staphyloccoccus aureus (MRSA) strains has presented a new challenge in antimicrobial medication. Linezolid is a new drug with potent activity on Gram-positive pathogens such as MRSA. The aim of the study was to investigate the in vitro activity of linezolid alone and in combination with imipenem, vancomycin or rifampicin to determine the most active therapy against MRSA strains. Twenty clinical MRSA strains were isolated from patients admitted to inpatient departments and outpatient clinics of Theodor Bilharz Research Institute. Standard strain MRSA ATCC 43300 was included as a control. The MICs of MRSA strains to linezolid, vancomycin, imipenem and rifampicin were evaluated using E test. Time-kill curve were used to assess the in vitro activity of linezolid (at 8x MIC) alone and in combination with imipenem (at 32x MIC), vancomycin or rifampicin (at 8x MIC). Scanning and transmission electron microscopy were performed to compare bacterial morphological alterations owing to the different combi- nations. Time-kill studies showed synergistic effect when linezolid combined with imipenem was tested against all the MRSA strains. Linezolid plus vancomycin or rifampicin combinations did not display any synergism or antagonism. Scanning and transmission electron microscopy observations confirmed the interactions observed in time kill experiments. Linezolid in combination with subinhibitory concentrations of imipenem can be bactericidal against MRSA strains and appears to be a promising combination for the treatment of MRSA infections. No synergistic activity was seen when the linezolid and vancomycin or rifampicin were combined. Linezolid could prevent the emergence of mutants resistant to rifampicin

Background: Development of non-invasive markers that can predict the stages of hepatic fibrosis w... more Background: Development of non-invasive markers that can predict the stages of hepatic fibrosis without resorting to repeated liver biopsies is still an important goal to evaluate the effectiveness of antifibrotic treatment. The present work investigates the value of the assessment of peripheral circulating reelin, in which the liver represents its prime source, as a marker for monitoring hepatic fibrogenesis. Methods: Seventy-four cases with chronic hepatitis positive for serum HCV RNA and 15 healthy volunteers were enrolled in this study. Assessment of reelin in the harvested serum and in 64 corresponding liver biopsies using immunofluorescence technique was done. The results were evaluated in relation to the stages and quantitative morphometric analysis of hepatic fibrosis as well as the serum levels of the validated biomarker hyaluronic acid. Results: Significant correlation was detected between the levels of serum reelin and the semiquantitative assessment of reelin immunoreactivity in liver tissue, the stages of hepatic fibrosis, the morphometrically determined collagen and serum hyaluronic acid with a correlation coefficient of 0.675, 0.623, 0.479, 0.772, respectively with p < 0.001. The sensitivity and the specificity of reelin for the determination of advanced (F2 + F3) and significant fibrosis (F2 – F4) were nearly comparable to the result of hyaluronic acid. In addition the area under curve (AUC)

An ultrastructural quantitative assessment of hepatic stellate cells (HSCs) was made in relation ... more An ultrastructural quantitative assessment of hepatic stellate cells (HSCs) was made in relation to hepatic fibrosis, apoptotic cellular changes, intracellular fat deposition, circulating inflammatory cells in the sinusoids, and the necroinflammatory activity in liver specimens of 33 patients proven to be positive for hepatitis C virus (HCV)-RNA by polymerase chain reaction with the intention that electron microscopy may throw more light on the role of HSCs in the complicated process of fibrogenesis. A detailed review concerning these parameters and observed evidence suggesting the potential properties of HSCs to recycle cellular debris into collagen fibers are reported.

Easy manipulation and preservation of cells in suspension through the different steps of sample p... more Easy manipulation and preservation of cells in suspension through the different steps of sample processing for electron microscopy examination is essential for proper diagnosis. The author used agarose gel as an embedding media for processing cells in suspension for electron microscopic examination. The AgarCyto cell block procedure of Kerstens et al. (J Histochem Cytochem. 2000; 48: 709 ö 718) was used to begin electron microscopic processing of exfoliated urothelial cells in voided urine or cells in suspension. Processing of agarose cell block simultaneously for light and electron microscopic examination represents a great advantage offered by this innovated technique.

Proper handling and processing of urine sample can greatly improve diagnostic sensitivity. This w... more Proper handling and processing of urine sample can greatly improve diagnostic sensitivity. This work investigates the value of agarose cell block technique in processing urine samples simultaneously for light and electron microscopic examination, with the prospect to enhance the quality of diagnosis. The material of this study consisted of 45 voided urine samples, processed for the performance of Papanicolaou-stained urine smears, agarose cell blocks paraffin sections stained with hematoxylin & eosin, and electron microscopy-contrasted ultrathin sections. The studied technique increases the sensitivity of urine cytology and opens a new prospect for cytomorphological study.

The present work highlights intracellular viral morphogenesis and virus–host cell interactions in... more The present work highlights intracellular viral morphogenesis and virus–host cell interactions
in patients proved to be infected with HCV. The material of this study consisted of 28 liver
biopsies taken from patients positive for serum HCV-RNA by polymerase chain reaction. Liver
biopsies were processed for light and electron microscopic examination. Ultrastructural findings of
this work supported a new hypothesis for the turnover of HCV to retrovirus and described the
presumed involved mechanism. This novel perception offers important insights that can explain
the vague mechanisms of HCV behavior in the infected hepatocytes

ABSTRACT Hepatitis C virus represents one of the rising causes of hepatocellular carcinoma (HCC).... more ABSTRACT
Hepatitis C virus represents one of the rising causes of hepatocellular carcinoma (HCC). Although
the early diagnosis of HCC is vital for successful curative treatment, the majority of lesions are
diagnosed in an irredeemable phase. This work deals with a comparative ultrastructural study of
experimentally gradually induced HCC, surgically resected HCC, and potential premalignant
lesions from HCV-infected patients, with the prospect to detect cellular criteria denoting premalignant
transformation. Among the main detected pathological changes which are postulated to
precede frank HCC: failure of normal hepatocyte regeneration with star shape clonal fragmentation,
frequent elucidation of hepatic progenitor cells and Hering canals, hepatocytes of different
electron density loaded with small sized rounded monotonous mitochondria, increase junctional
complexes bordering bile canaliculi and in between hepatocyte membranes, abundant cellular
proteinaceous material with hypertrophied or vesiculated rough endoplasmic reticulum (RER),
sequestrated nucleus with proteinaceous granular material or hypertrophied RER, formation of
lipolysosomes, large autophagosomes, and micro-vesicular fat deposition.
In conclusion, the present work has visualized new hepatocytic division or regenerative
process that mimic splitting or clonal fragmentation that occurs in primitive creature. Also, new
observations that may be of value or assist in predicting HCC and identifying the appropriate
patient for surveillance have been reported. Moreover, it has pointed to the possible malignant
potentiality of liver stem/progenitor cells.
For reliability, the results can be subjected to cohort longitudinal study.