Sonia Osorio - Academia.edu (original) (raw)

Papers by Sonia Osorio

Yield quantitative trait loci from wild tomato are predominately expressed by the shoot

Ethylene is involved in strawberry fruit ripening in an

Fruit ripening is a highly coordinated developmental process that coincides with seed maturation.... more Fruit ripening is a highly coordinated developmental process that coincides with seed maturation. The ripening process is regulated by thousands of genes that control progressive softening and/or lignification of pericarp layers, accumulation of sugars, acids, pigments, and release of volatiles. Key to crop improvement is a deeper understanding of the processes underlying fruit ripening. In tomato, mutations blocking the transition to ripe fruits have provided insights into the role of ethylene and its associated molecular networks involved in the control of ripening. However, the role of other plant hormones is still poorly understood. In this review, we describe how plant hormones, transcription factors, and epigenetic changes are intimately related to provide a tight control of the ripening process. Recent findings from comparative genomics and system biology approaches are discussed.

Climate change is affecting berry cultivation. Goodberry Project focuses on the improvement of be... more Climate change is affecting berry cultivation. Goodberry Project focuses on the improvement of berry crops, studying fruit quality in different cultivars and climatic zones. Goodberry aims to discover which berry cultivars have the highest quality, in order to produce year-round, tasty, healthy fruits. 1Instituto de Hortofruticultura Subtropical y Mediterránea “La Mayora” – University of MalagaConsejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC), Department of Molecular Biology and Biochemistry; Campus de Teatinos, 29071, Málaga, Spain. 2Sant'Orsola Società Cooperativa Agricola, Via Lagorai, 131,38057 Pergine Valsugana (TN), Italy. 3 Ciref Création Variétale Fraises Fruits Rouge. Douville, 24140, Dordogne, France. 4Instytut Ogrodnictwa (INHORT), Skierniewice, Pologne. 5Hochschule Geisenheim University, 65366 Geisenheim, Germany. 6NIBIO, Norwegian Institute of Bioeconomy Research, NO-1431, Ås, Norway. 7INRA, UMR1332 BFP, F-33140 Villenave d’Ornon, France, Université de...

1Instituto de Hortofruticultura Subtropical y Mediterránea “La Mayora”, University of Malaga-Cons... more 1Instituto de Hortofruticultura Subtropical y Mediterránea “La Mayora”, University of Malaga-Consejo Superior de Investigaciones Científicas, Department of Molecular Biology and Biochemistry, Campus de Teatinos, 29071 Malaga, Spain. 2Max-Planck-Institute für Molekulare Planzenphysiologie, Am Mühlenberg 1, 14476 Golm, Germany. 3Instituto Andaluz de Investigación y Formación Agraria y Pesquera (IFAPA), Centro de Huelva. Julio Caro Baroja s/n, Huelva, Spain. 4IFAPA, Centro de Churriana, Cortijo de la Cruz S/N, Churriana, 29140 Malaga, Spain. * dpott@uma.es; ¶ sosorio@uma.es

Universidad de Malaga. Campus de Excelencia Internacional Andalucia Tech. The work was supported ... more Universidad de Malaga. Campus de Excelencia Internacional Andalucia Tech. The work was supported by the MINECO (grant AGL2012-40066-C02-02 ; Spain). SO aknowledges the support by Spanish Ministry of Science and Innovation (Ramon and Cajal contract, RYC2011-09170). DP has received a predoctoral grant from MINECO (grant BES-2013-062856). JJM and IA has been supported by the grant AGL2012-40066-C02-01). The authors also aknowlegde the support by the University de Malaga, Campus de Excelencia Internacional de Andalucia.

Figure 2: Principal component analysis (PCA) showing samples distribution for primary metabolites... more Figure 2: Principal component analysis (PCA) showing samples distribution for primary metabolites (A) and volatiles (B). Shapes indicate different cultivars of strawberry, raspberry and blackcurrant, respectively, while colors represent different countries where the berries were grown in 2017. Labels A and B indicate two different times of harvest during 2017. 1Instituto de Hortofruticultura Subtropical y Mediterránea “La Mayora” – University of MalagaConsejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC), Department of Molecular Biology and Biochemistry; Campus de Teatinos, 29071, Málaga, Spain. 2Sant'Orsola Società Cooperativa Agricola, Via Lagorai, 131,38057 Pergine Valsugana (TN), Italy. 3 Ciref Création Variétale Fraises Fruits Rouge. Douville, 24140, Dordogne, France. 4Instytut Ogrodnictwa (INHORT), Skierniewice, Pologne. 5Hochschule Geisenheim University, 65366 Geisenheim, Germany. 6NIBIO, Norwegian Institute of Bioeconomy Research, NO-1431, Ås, Norway. 7INRA, UM...

Postharvest Physiology and Biochemistry of Fruits and Vegetables, 2019

Methods in Molecular Biology, 2018

Plants synthesize and emit a large range of volatile organic compounds (VOCs) that play important... more Plants synthesize and emit a large range of volatile organic compounds (VOCs) that play important roles in their interactions with the environment, from attracting pollinators and seed dispersers to protectants such as repellants and pathogen inhibitors. As such, the development of techniques for headspace collection of volatiles in combination with gas chromatography-mass spectrometry (GC-MS) has an important impact on our understanding of the biosynthesis of plant VOCs. Furthermore, knowledge of the plant VOCs can be valuable in relation to plant breeding for improving fruit flavor or enhancing resistance to insects or pathogens. This chapter describes a reliable method for extracting volatile compounds by headspace solid-phase microextraction (HS-SPME), and separate and detect them by GC-MS.

The Plant Journal, 2020

Given the potential health benefits (and adverse effects), of polyphenolic and steroidal glycoalk... more Given the potential health benefits (and adverse effects), of polyphenolic and steroidal glycoalkaloids in the diet there is a growing interest in fully elucidating the genetic control of their levels in foodstuffs. Here we carried out profiling of the specialized metabolites in the seeds of the Solanum pennellii introgression lines identifying 338 putative metabolite quantitative trait loci (mQTL) for flavonoids, steroidal glycoalkaloids and further specialized metabolites. Two putative mQTL for flavonols and one for steroidal glycoalkaloids were cross-validated by evaluation of the metabolite content of recombinants harboring smaller introgression in the corresponding QTL interval or by analysis of lines from an independently derived backcrossed inbred line population. The steroidal glycoalkaloid mQTL was localized to a chromosomal region spanning 14 genes including a previously defined steroidal glycoalkaloid gene cluster. The flavonoid mQTL was further validated via the use of transient and stable overexpression of the Solyc12g098600 and Solyc12g096870 genes which encode seed specific UDP-glycosyltransferases (UGTs). The results are discussed in the context of our understanding of the accumulation of polyphenols and steroidal glycoalkaloids and how this knowledge may be incorporated into breeding strategies aimed at improving nutritional aspects of plants as well as in fortifying them against abiotic stress.

Plant Biotechnology Journal, 2019

Phytochemistry, Jan 25, 2017

The cell wall invertase gene (LIN5) was reported to be a key enzyme influencing sugar uptake of t... more The cell wall invertase gene (LIN5) was reported to be a key enzyme influencing sugar uptake of tomato (Solanum lycopersicum) fruit. It was additionally revealed to be a key regulator of total soluble solids content in fruit as well as for reproductive development, being mainly involved in flower development, early fruit and seed development but also in ripening. Here, we demonstrate that silencing of the LIN5 gene promotes changes affecting fruit cuticle development which has a direct effect on postharvest properties. Transformants were characterized by reduced transpirational water loss in mature fruits accompanied by several other changes in the cuticle. Quantitative chemical composition, coupled with microscopy of isolated cuticle fruits revealed that the cuticle of the transformants were characterized by an increase of the thickness as well as significant increase in the content of cuticle components (cutin, phenolic compounds, and waxes). Furthermore, detailed analysis of the ...

Methods in Molecular Biology, 2016

Phytohormones are key signaling molecules that coordinate plant growth and development through a ... more Phytohormones are key signaling molecules that coordinate plant growth and development through a range of complex interactions. Since the vast majority of plant responses to given stimuli result, amongst other factors, from a crosstalk between hormones, simultaneous analysis of multiple hormones is vital to improve our understanding of these interactions. This chapter describes a sensitive, reliable, and inexpensive method for quantification of multiple phytohormones by gas chromatography-mass spectrometry (GC-MS).