Ole Sonne - Academia.edu (original) (raw)

Papers by Ole Sonne

A protocol has been developed for maintaining isolated rat adipose cells in primary tissue cultur... more A protocol has been developed for maintaining isolated rat adipose cells in primary tissue culture. Using this protocol, cells remain fully viable and responsive to insulin for at least 24 h, as assessed by measuring 3-0-methylglucose transport, lipogenesis from [U-14 C]glucose, and the incorporation of [ 35 S]methionine into total membrane protein. The acute insulin-induced internalization of its own receptor was then examined by biosynthetically labeling cells in culture with either [ 35 S]methionine or [ 3 H]glucosamine, maximally inducing receptor internalization with a 30-min incubation in the presence of saturating insulin, and preparing plasma and low-density microsomal membrane fractions by differential ultracentrifugation. Receptors were immunoprecipitated with anti-receptor antiserum, and the receptor subunits separated by NaDodSO 4-PAGE under reducing conditions and analyzed by autoradtography. When cells not acutely treated with insulin are examined, both the 135K a-and 95K p-receptor subunits are prominently labeled in the plasma membrane fraction, but only faintly labeled in the low-density microsomal membrane fraction. Following the induction of maximal acute receptor internalization, both subunits are decreased by 20-30% in the plasma membrane fraction and concomitantly increased in the low-density microsomal membrane fraction. However, the relative molecular weights and labeling intensities of the two subunits remain constant and correspond to those observed in the biosynthetically labeled human lymphocyte receptor. A minor band of M r-190K is also labeled, but its labeling intensity is similar in the two membrane fractions from basal cells and does not change in response to insulin. This band has not been previously detected in the rat adipose cell, but is thought to represent either a minor receptor compo-From the Cellular Metabolism and Obesity Section (IAS. and S.W.C.) and Diabetes Branch (J.A.H.

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Hoppe-Seyler's Zeitschrift für Physiologische Chemie, 1981

The binding affinity to insulin receptors in isolated rat adipocytes at 37 °C of the four isomers... more The binding affinity to insulin receptors in isolated rat adipocytes at 37 °C of the four isomers of [ 125 I]monoiodoinsulin was ranked as B 26 > B 16 = A14 > A19. It was demonstrated that the difference in affinity was mainly due to a change in the association rate constant, rather than in the dissociation rate constant. At steady state in the binding process the fraction of cellassociated 12S l-activity eluting from a Sephadex G-50 Fine column at a position identical to that of iodoinsulin was > 90% and independent of the position of the iodine. Zusammenfassung: Die Bindungsaffinität der vier isomeren [ 125 I]Monoiodinsuline an die Insulinrezeptoren isolierter Rattenadipozyten bei 37 °C nimmt in der Reihe B26 > B16-A14 > A19 ab. Der Affinitätsunterschied beruht hauptsächlich auf Änderung der Assoziationsgeschwindigkeits-Konstante, weniger auf der der Dissoziationsgeschwindigkeit. Im Fließgleichgewicht des Bindungsprozesses betrug der Anteil der zellgebundenen 125 I-Aktivität, die von einer Sephadex-G-50-Fine-Säule in gleicher Position wie lodinsulin eluierte, weniger als 90%, unabhängig von der Stellung des lods.

Journal of Chromatography A, 1984

ABSTRACT

Physiological Reviews, Oct 1, 1988

Receptor-mediated internalization and degradation of insulin-like growth factors, IGF-I and IGF-1... more Receptor-mediated internalization and degradation of insulin-like growth factors, IGF-I and IGF-11, were studied in primary cultures of neonatal rat astrocytes. Surface-bound IGF-I1 was rapidly internal-ize& and 80% of cell-associated radioactivity was located intracellularly after 30 min. IGF-I was internalized at a slower rate, and only 40% of cell-associated radioactivity was inside the cell after 30 min. A pulse-chase experiment demonstrated that 55% and 70% of internalized IGF-I and IGF-11, respectively, was degraded to free amino acids after a 3-hr chase. Lysosomal and protease inhibitors had different effects on the binding, internalization, and processing of IGF-I and IGF-11. Inhibition of lysosomal acidification by chloroquine increased the amounts of surface-bound IGF-I1 and intracellular IGF-I and reduced the degradation of IGF-I. The chelating agent phenanthroline increased the surface binding of IGF-I and IGF-I1 and internalization of IGF-I1 and reduced the degradation of IGF-I and IGF-11. Finally, receptor-bound IGF-I1 on the cell surface was decreased with increasing cell density, whereas IGF-I binding was unaltered. Our data suggest that cellsurface expression of IGF-I receptors and IGF-I1 receptors is regulated by different mechanisms and that receptor-bound IGF-I and IGF-I1 are trafficked and processed by different intracellular pathways in neonatal rat astrocytes.

Aarhus University Press eBooks, Nov 12, 2011

Publisher Summary This chapter discusses how insulin interacts with receptors in rat adipocytes a... more Publisher Summary This chapter discusses how insulin interacts with receptors in rat adipocytes and how it elicits one of its important effects: the increase in the permeability of the plasma membrane to glucose. It discusses three processes: (1) the receptor binding of 125 I-labelled insulin that is used as a tracer for insulin, (2) the effect of insulin on transmembrane transport of 3-O-methylglucose; this glucose analog is used because it is not phosphorylated and metabolized, and (3) the degradation of insulin that occurs in two ways in a suspension of rat adipocytes in vitro . Receptor-bound insulin is degraded in adipocytes incubated at near-physiological temperature and pH. The binding of insulin to its receptor is, therefore, not a bimolecular reversible reaction. Insulin in a very high concentration requires about 50 seconds toincrease the permeability of adipocytes to methylglucose. Insulin increases the maximal transport velocity possibly by increasing the number of available carriers.

Aarhus University Press eBooks, Oct 1, 2013

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Ugeskrift for laeger, Jan 20, 1977

International Journal of Peptide and Protein Research, 2009

Monoiodoinsulin was prepared using ion exchange chromatography. The isolated monoiodoinsulin show... more Monoiodoinsulin was prepared using ion exchange chromatography. The isolated monoiodoinsulin showed on polyacrylamide gel electrophoresis two bands with different intensities related to the initial method of iodination. Each of the two bands were isolated from the gel, and determination of the iodine distribution among the tyrosyl groups showed that one band contained monoiodoinsulin substituted in Tyr A19 contaminated with monoiodoinsulin substituted in the B‐chain. The other band contained essentially A14 monoiodoinsulin. Polyacrylamide gel electrophoresis is a convenient method to prepare homogeneous A14 monoiodoinsulin with biological activity indistinguishable from that of native insulin.

Membrane Proteins, 1978

Dansk medicinhistorisk arbog, 2016

Three plague pandemics plus several epidemics have ravaged the world. The three pandemics were ch... more Three plague pandemics plus several epidemics have ravaged the world. The three pandemics were characterised by the role shipping played in spreading of the plague. The third pandemic, which began in southern China in the 1850s, was carried out of Hong Kong in 1894 to all continents by steamships. The oldest known documents mentioning quarantine as a precaution against epidemics dates back to 1127 in Venice. During the second pandemic, the Black Death, quarantine was systematised. During the third pandemic gassing of the ships was introduced by burning sulphur. Later hydrogen cyanide, carbon monoxide and other toxic gasses have been applied. In many harbours the use of rat shields were made compulsory in the beginning of the 20th century. The French bacteriologist Alexandre Emile Jean Yersin isolated in 1894 and identified Yersinia pestis as the contagious agent in Hong Kong despite obstructions from the British authorities who favoured Shibasaburo Kitasato from Japan. Four years la...

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Dansk medicinhistorisk arbog, 2015

The Scottish physiologist John Scott Haldane (1860-1936) spent most of his professional career in... more The Scottish physiologist John Scott Haldane (1860-1936) spent most of his professional career in Oxford after graduating from the medical school in Edinburgh. He was deeply involved in applying basic science on problems in society but also making these problems guide his choice of projects in his experimental work. Thus, he has demonstrated that the increased contents of carbon dioxide in dwellings, schools, and factories was of less importance than the high contents of bacteria and fungal spores, and that even the foul air in the sewers was less harmful than that in crowded dwellings. He demonstrated that most miners did not die of lack of oxygen or trauma after colliery accidents but of carbon monoxide poisoning. The miners had relied on the ability of their candle or lamp to burn, but this would not be influenced by the presence of carbon monoxide. Thus, he introduced the canaries, which due to their small size and correspondingly relatively higher metabolism would faint about 2...