Srikrushna Mahapatra - Academia.edu (original) (raw)
Papers by Srikrushna Mahapatra
International Journal of Clinical Biochemistry and Research, 2016
Background and Objective: Intrahepatic cholestasis of pregnancy (ICP) is seen usually in 3 rd tri... more Background and Objective: Intrahepatic cholestasis of pregnancy (ICP) is seen usually in 3 rd trimester of pregnancy. Overall incidence is 1.2-1.5% of Indian pregnant women. The biochemical abnormalities often include raised liver enzymes, conjugated bilirubin and bile acids. ICP can complicate perinatal outcomes to a significant extent. The present study was conducted to find out the types and incidence of perinatal outcomes in pregnancy complicated with ICP diagnosed by abnormal LFTS (liver function tests), to help the clinician in initiating timely treatment. Materials and methods: In the present study, 51 pregnant women diagnosed with ICP and 49 number of age and gravida matched normal pregnant women were included as cases and controls respectively. LFT and Total bile acids were estimated by standard biochemical methods. Both cases and controls were followed to evaluate perinatal outcomes after 7 days of postpartum.
Journal of Integrative Medicine and Research
Biomedicine, 2021
Individual’s genetic makeup best describes the properties regarding its growth and development. I... more Individual’s genetic makeup best describes the properties regarding its growth and development. It is stored and passed on to generations and is in dynamic equilibrium with the environmental and other non-living factors. The most predominant environmental stimuli are diet/nutrition. Diet/nutrition interacts and modulates varying underlying molecular mechanisms central to various physiological functions basically at three different levels: genome, proteome, and metabolome. Advances in genomic studies are paving the way to the development of scientific insights into nutritional sciences. Nutrigenetics and nutrigenomics are closely associated but two different areas of nutritional research. Both the fields involved the study of the implication between nutrition, metabolism, and genetic mechanism. The primary goal is to pinpoint nutrient-dependent health characteristics and nutrition dependent diseases. Another important area connected to these sciences concerns food composition and pe...
Journal of Family Medicine and Primary Care, 2021
Background: With the change in the National Cholesterol Education Program ATP III guidelines, the... more Background: With the change in the National Cholesterol Education Program ATP III guidelines, the risk of developing atherosclerosis has been now focused on total cholesterol and low-density lipoprotein (LDL) cholesterol levels. Different treatment modalities are now targeted at lowering LDL cholesterol values. Hence greater emphasis is now led on the accurate and precise measurement of LDL cholesterol. Beta-quantification, though, is the best reference method for LDL cholesterol estimation, it has the disadvantage of being inconvenient in our routine practice. The new generation direct homogenous assay is now the method of choice. But being more expensive, various calculated methods have now been developed. This study is an attempt to compare different calculated formula with direct cholesterol assessment and to find out the best one. Materials and Methods: We compared LDL cholesterol measured by direct homogenous assay with the data mining approach (DM) and another calculated formula [Friedewald's Formula (FF) and Anandaraja Formula (AF)] in 266 samples with age greater than 18 years. Enrolled participants were divided into seven groups based upon their TG levels. Mean, percentage difference, and the correlation coefficient was assessed between calculated and direct LDL. Bland–Altman analysis was done to see the agreement between calculated vs direct LDL. All formulas were assessed among various TG levels with direct LDL by the Wilcoxon sign rank test. Result: 1% level of significance was found between calculated and direct LDL with TG < 600 mg/dl. Mean and the percentage difference between direct and calculated LDL was lowest with the DM approach. Bland–Altman plot shows the best agreement of the DM approach with direct LDL. Conclusion: This study indicates that the DM approach is closer to direct LDL compared to FF & AF.
Journal of Diabetes Mellitus
Background and objectives: Vitamin D is a fat-soluble vitamin belonging to secosteriods. It is re... more Background and objectives: Vitamin D is a fat-soluble vitamin belonging to secosteriods. It is responsible for intestinal absorption and bioavailibity of calcium and phosphorus.Synthesisof vitamin D in response to ultraviolet rays in skin depends on latitude, atmospheric pollution, clothing, skin pigmentation, sunscreen usage, duration and time of exposure to sunlight. Deficiency causes rickets, defective bone mineralization, osteomalacia and various non skeletal diseases such asrheumatoid arthritis, diabetes mellitus, coronary artery disease and cancer. Recent studies have suggested a deficiency state in Indian populationinspite of ample sunshine. Hence, this study was designed to estimate the prevalence of vitamin D deficiency in adults in the coastal regions of Odisha, India Material and methods: This cross-sectional study included 3056 conducted among healthy population of Cuttack, a coastal district of Odisha. All the individuals were tested for serum 25(OH)D level by electroch...
Background and objectives: Prediabetic is the precursor state of the disease Diabetic Mellitus (D... more Background and objectives: Prediabetic is the precursor state of the disease Diabetic Mellitus (DM).It is a metabolic diathesis with high blood sugar and dyslipidaemia. This increases the risk for the development of DM and cardiovascular diseases. The prediabetic state is classified into: (1) the impaired fasting glycemia (IFG) and (2) impaired glucose tolerance (IGT). WHO has defined impaired fasting glucose level as 110 to 125mg% and impaired glucose tolerance as that 2hour plasma glucose to be within the range of 140 to 200 mg% after an oral glucose load of 75grams. Hence, this study was designed to(1) evaluate the role oxidative stress, endothelial dysfunction , inflammation (2) find diagnostic markers for early detection of changes in prediabetes to prevent DM. Material and method: This case control study included 112prediabetic individuals and an equal number of matched healthy volunteers. The FBG, lipid profile, HbA1c and CRP were estimated. We used ferrous oxidation in xylen...
Background and objective: The serum Low density Lipoprotein Cholesterol Concentration can be esti... more Background and objective: The serum Low density Lipoprotein Cholesterol Concentration can be estimated by Freidewald formula and also by the direct assay methods by a spectrophotometer. The National Cholesterol Education program recommends the direct assay for estimating serum LDL-C concentration, but it has not been evaluated whether there is any difference between Freidwald formula & LDL-C direct assay methods in assessing Patients for CVD ( cardio vascular Disease). Methods: This study included the samples of 28,346 patients whose serum triglyceride level was less than 400 mg%. The study was carried in the month of January to May 2018. Fasting samples and non fasting samples were collected for measurement of lipid profile and commercial kits adapted to autoanalyser were used for the direct assay and Friedewald formula was used to calculate the value. Results: Serum LDL-C levels estimated by the direct method and Friedewald formula had a significant positive correlation (r = 0.968...
According to recent findings both X and Y chromosome could have evolved from autosomal ancestors ... more According to recent findings both X and Y chromosome could have evolved from autosomal ancestors about 300 million years ago. In humans, the typical male has a diploid karyotype of 46 chromosomes i.e. 22 pairs of autosomes and XY pair of gonosomes(46,XY).A standard female karyotype is 46 XX.Thus humans are dimorphic: there is one type of humans who are males and the other type is female. The basis of sexual dimorphism in mammals derives from evolution of sex chromosomes (1). Probably a failure occurred in homologous recombination which resulted in the formation of a small area that was not identical to the two participating chromosomes. The presence or absence of this region coincided with a different pattern of development that altered androgen activity resulting in a sex determining role. In all living mammalians to day, this area in Y chromosome appears to retain the regulatory function and therefore described as the sex Determining Region of Y chromosome or SRY gene(2).But sexua...
BACKGROUND AND OBJECTIVES: Renal dysfunction is end organ damage in hypertension. We investigated... more BACKGROUND AND OBJECTIVES: Renal dysfunction is end organ damage in hypertension. We investigated the association of oxidative stress with estimated glomerular filtration rate in hypertension. METHODS: A total of 116 hypertensive individuals (age 47 ± 10.2 years; 68% males; BMI < 25; without diabetes mellitus, CVD) were included. All the hypertensive subjects were albustix negative. Fasting blood glucose, lipid profile, urea and creatinine was estimated. Serum oxidant load was estimated by ferrous oxidation products in xylenol orange version 2(FOX2) and antioxidant power of serum was estimated by ferric reducing capacity, FRAP assay. Data is represented as mean± SD. Data was analysed by unpaired two-tailed students t test, Pearson's correlation and linear regression. The estimated GFR was calculated by simplified modification of diet in renal disease study prediction equation and Cockroft-Gault formula. RESULT: Systolic and diastolic blood pressure, lipid profile, serum creatinine and oxidant load was significantly higher in hypertensive subjects (p < 0.001). Total Antioxidant status and estimated GFR was significantly lower in hypertensive subjects. A significant negative correlation was present between oxidative stress and eGFR. INTERPRETATION AND CONCLUSION: Among hypertensive subjects with preserved renal function increased oxidative stress is associated with decreased eGFR. Whether estimation of oxidative stress and adjuvant antioxidant therapy are better prognostic markers requires further research.
Laboratory Medicine, 2022
Objective Upregulation of matrix metalloproteinase-7 (MMP-7) is associated with hypertension and ... more Objective Upregulation of matrix metalloproteinase-7 (MMP-7) is associated with hypertension and kidney fibrosis, which can progress to chronic kidney disease (CKD). Currently, kidney fibrosis is only detectable by an invasive procedure. Therefore, we set out to determine whether MMP-7 can act as a noninvasive biomarker in patients with hypertension to enable early detection of kidney fibrosis. Materials and Methods Diagnosed patients with hypertension and control patients were sampled. We diagnosed CKD using clinical and laboratory parameters. Serum urea, creatinine, urinary microalbumin, the albumin-to-creatinine ratio, and urinary MMP-7 were analyzed. Results The 195 patients with hypertension had significantly elevated MMP-7. Of these patients, 166 had MMP-7 >25.8 μg/L, whereas only 29 had MMP-7 <25.8 μg/L. Thirty-two patients with hypertension showed features of CKD, all of whom had urinary MMP-7 >25.8 μg/L. However, the urinary MMP-7 level did not differ with the seve...
International Journal of Diabetes in Developing Countries, 2012
ABSTRACT Inflammation, endothelial dysfunction, and oxidative stress are postulated to be the pri... more ABSTRACT Inflammation, endothelial dysfunction, and oxidative stress are postulated to be the principal events in the pathogenesis of type 2 diabetes mellitus and its vascular complications. Serum Nitric Oxide metabolites (NOX) and high sensitivity C-reactive protein (hs-CRP) were measured in eighty non-obese, type 2 diabetic males (40 to 65 years) without (group B, N040) and with vascular disease [16 retinopathy (group C), 24 hypertension (group D)]; and compared with forty healthy, age and sex matched control subjects (group A). The mean age of diabetic patients and healthy controls was 49.5±5.80 vs. 51.0±7.15 years, respectively (P00.212). Diabetic group had higher Fasting Plasma Glucose (FPG), hs-CRP, and NOX levels than control group (135.3±28.69 vs. 96.7±10.46; 5.1±2.59 vs. 1.7± 0.54; 61.1±15.67 vs. 37.1±3.69, respectively, P00.001). When compared with diabetics without complication (53.7±10.37), levels of NOX were significantly higher (P0 0.001) both in retinopathy (74.5±19.39) and hypertension (64.4±13.60) group, whereas that of hs-CRP (4.4±2.85 vs.6.5±2.93, P00.015) differed only in retinopathy group. There was no difference between retinopathy and hypertension group. There was significant (P<0.05) positive correlation between age, duration of diabetes, hs-CRP, and NOX; hs-CRP and NOX among group B and C. A negative correlation was noted between hs-CRP and NOX (r0−0.068, P0 0.752) among hypertensives. By multivariate regression analysis, FPG, hs-CRP and NOX were found to be independent indicators of complications after adjustment for age and duration of diabetes. This study reveals that NOX and hs- CRP are important biomarkers in type 2 diabetes mellitus and its associated vascular complications.
Introduction: Impaired fibrinolysis and low-grade systemic inflammation are postulated to be asso... more Introduction: Impaired fibrinolysis and low-grade systemic inflammation are postulated to be associated with the pathogenesis of type 2 diabetes mellitus (T2DM) with its micro and macrovascular complications. However, this mechanism is complex and poorly understood. Materials and Methods: Fasting plasma glucose (FPG), plasma fibrinogen, serum high-sensitivity C-reactive protein (hs-CRP), and lipid parameters were measured among eighty, nonobese, adult type 2 diabetic males (age: 40–65 years) (without complications [ n = 40, Group 2], with retinopathy [ n = 16, Group 3], with hypertension [ n = 24, Group 4]), and compared with age- and gender-matched healthy controls ( n = 40, Group 1). Results: The mean age of subjects in Groups 1, 2, 3, and 4 were 51 ± 7.2, 49 ± 5.3, 51.5 ± 7.2, and 49.3 ± 5.3 years, respectively ( P = 0.33). Fibrinogen, FPG, and hs-CRP were significantly higher among diabetics than controls ( P P = 0.01) among diabetics with complications than without complication...
Aim and Objective: Omentin-1 is a novel adipokine expressed in visceral adipose tissue and has be... more Aim and Objective: Omentin-1 is a novel adipokine expressed in visceral adipose tissue and has been reported to be negatively associated with insulin resistance (IR). The present study aimed at assessing the correlation of serum Omentin-1 with IR in Type II Diabetes mellitus patients as measured by Homeostasis Model Assessment (HOMA-IR). There has been very few reports (if any) from Indian subcontinent on the role of Omentin in IR. Materials and Methods: It is a case control study, fifty cases of Type II DM patients and equal number of age and sex matched controls were included in the study. Fasting serum Omentin, fasting serum insulin were estimated by ELISA using commercially available ELISA kit and fasting blood sugar by glucose oxidase method by using EM360 autoanalyzer. HOMA-IR score was calculated as HOMA-IR = [Fasting Glucose (mg/dl) × Fasting Insulin (μIU/ml)] / 405. Serum Omentin-1 was correlated with FBS, serum insulin and HOMA-IR using SPSS version-20. Results: Significan...
Biomedicine
Background: Secondary antiphospholipid syndrome (APLS) and rise in antiphospholipid antibody (APL... more Background: Secondary antiphospholipid syndrome (APLS) and rise in antiphospholipid antibody (APLA) has been linked to the development of Disseminated Intravascular Coagulation in COVID-19. But still controversies exist regarding the increase in APLA in COVID -19. Hence, the present study aimed to estimate the levels of APLA in COVID patients and its relationship with the severity of the disease. Materials and Methods: 40 RT PCR positive COVID-19 cases and normal control were recruited for the study. Biochemical and hematological findings were compared in both the groups. COVID-19 patients were further subdivided into survivor Vs non-survivor and based upon the CT findings of thorax they were grouped with vs without CT findings. Results: CRP, PCT, ESR were found to be significantly increased in COVID-19 patients. IgM & IgG APLA antibody (3.02±1.32 U/ml & 3.54±1.85 U/ml) were found to be within normal range in COVID cases. APLA did not show any correlation with serum ferritin, CRP, ...
IOSR Journal of Pharmacy and Biological Sciences, 2012
Background and objectives: Recent studies imply an increasing association of oxidative stress in ... more Background and objectives: Recent studies imply an increasing association of oxidative stress in the pathogenesis of hypertension. This study was designed to evaluate oxidative stress in hypertensive subjects and to assess the correlation of antioxidant status with the severity of hypertension. Material and methods: Ninety-six hypertensive cases were divided into three groups based on severity of hypertension as per JNC VII classification. Lipid profile, antioxidant power of serum(FRAP assay) and oxidant load of serum(FOX2) of hypertensive cases were compared with an equal number of age and sex matched healthy normotensive controls. Data was analysed by Student's t test and Pearson's correlation. Result: Lipid profile, oxidant load was significantly higher in hypertensive patients in comparison to controls (P<0.001). Antioxidant status was lower in hypertensives as compared to controls. A significant correlation was observed between lipid profile vs. oxidative stress and hypertension vs. low antioxidant level (r=0.835, P<0.001) Conclusion: Above findings demonstrate a strong association between blood pressure, dyslipidaemia and oxidative parameters shows a possible role of oxidative stress in the severity of hypertension.
Biomedicine
Background: Secondary antiphospholipid syndrome (APLS) and rise in antiphospholipid antibody (APL... more Background: Secondary antiphospholipid syndrome (APLS) and rise in antiphospholipid antibody (APLA) has been linked to the development of Disseminated Intravascular Coagulation in COVID-19. But still controversies exist regarding the increase in APLA in COVID -19. Hence, the present study aimed to estimate the levels of APLA in COVID patients and its relationship with the severity of the disease. Materials and Methods: 40 RT PCR positive COVID-19 cases and normal control were recruited for the study. Biochemical and hematological findings were compared in both the groups. COVID-19 patients were further subdivided into survivor Vs non-survivor and based upon the CT findings of thorax they were grouped with vs without CT findings. Results: CRP, PCT, ESR were found to be significantly increased in COVID-19 patients. IgM & IgG APLA antibody (3.02±1.32 U/ml & 3.54±1.85 U/ml) were found to be within normal range in COVID cases. APLA did not show any correlation with serum ferritin, CRP, ...
Biomedicine
Introduction and Aim: Serum creatinine (SCr) and estimated glomerular filtration rate (eGFR) give... more Introduction and Aim: Serum creatinine (SCr) and estimated glomerular filtration rate (eGFR) gives the idea of overall functional status of kidney. Measurement of SCr by different method and discordant result between them can misclassify different stages of chronic kidney disease (CKD). Many of the tertiary care health centre, SCr measured by Jaffe method is still the method of choice because of its cost effectiveness even if it is more susceptible for interference. Our aim was to measure SCr level by both Jaffe’s & enzymatic method and analysing the discordance rate of eGFR and their effect in staging of kidney disease in CKD patients. Materials and Methods: In this observational study 330 serum sample were analysed for creatinine by Jaffe’s and enzymatic methods in a fully automated analyser using commercially available kits. Modification of Diet in Renal Disease (MDRD) formula was used for estimating GFR. Results: In 330 subjects eGFR values calculated on incorporating SCr were f...
International Journal of Clinical Biochemistry and Research, 2016
Background and Objective: Intrahepatic cholestasis of pregnancy (ICP) is seen usually in 3 rd tri... more Background and Objective: Intrahepatic cholestasis of pregnancy (ICP) is seen usually in 3 rd trimester of pregnancy. Overall incidence is 1.2-1.5% of Indian pregnant women. The biochemical abnormalities often include raised liver enzymes, conjugated bilirubin and bile acids. ICP can complicate perinatal outcomes to a significant extent. The present study was conducted to find out the types and incidence of perinatal outcomes in pregnancy complicated with ICP diagnosed by abnormal LFTS (liver function tests), to help the clinician in initiating timely treatment. Materials and methods: In the present study, 51 pregnant women diagnosed with ICP and 49 number of age and gravida matched normal pregnant women were included as cases and controls respectively. LFT and Total bile acids were estimated by standard biochemical methods. Both cases and controls were followed to evaluate perinatal outcomes after 7 days of postpartum.
Journal of Integrative Medicine and Research
Biomedicine, 2021
Individual’s genetic makeup best describes the properties regarding its growth and development. I... more Individual’s genetic makeup best describes the properties regarding its growth and development. It is stored and passed on to generations and is in dynamic equilibrium with the environmental and other non-living factors. The most predominant environmental stimuli are diet/nutrition. Diet/nutrition interacts and modulates varying underlying molecular mechanisms central to various physiological functions basically at three different levels: genome, proteome, and metabolome. Advances in genomic studies are paving the way to the development of scientific insights into nutritional sciences. Nutrigenetics and nutrigenomics are closely associated but two different areas of nutritional research. Both the fields involved the study of the implication between nutrition, metabolism, and genetic mechanism. The primary goal is to pinpoint nutrient-dependent health characteristics and nutrition dependent diseases. Another important area connected to these sciences concerns food composition and pe...
Journal of Family Medicine and Primary Care, 2021
Background: With the change in the National Cholesterol Education Program ATP III guidelines, the... more Background: With the change in the National Cholesterol Education Program ATP III guidelines, the risk of developing atherosclerosis has been now focused on total cholesterol and low-density lipoprotein (LDL) cholesterol levels. Different treatment modalities are now targeted at lowering LDL cholesterol values. Hence greater emphasis is now led on the accurate and precise measurement of LDL cholesterol. Beta-quantification, though, is the best reference method for LDL cholesterol estimation, it has the disadvantage of being inconvenient in our routine practice. The new generation direct homogenous assay is now the method of choice. But being more expensive, various calculated methods have now been developed. This study is an attempt to compare different calculated formula with direct cholesterol assessment and to find out the best one. Materials and Methods: We compared LDL cholesterol measured by direct homogenous assay with the data mining approach (DM) and another calculated formula [Friedewald's Formula (FF) and Anandaraja Formula (AF)] in 266 samples with age greater than 18 years. Enrolled participants were divided into seven groups based upon their TG levels. Mean, percentage difference, and the correlation coefficient was assessed between calculated and direct LDL. Bland–Altman analysis was done to see the agreement between calculated vs direct LDL. All formulas were assessed among various TG levels with direct LDL by the Wilcoxon sign rank test. Result: 1% level of significance was found between calculated and direct LDL with TG < 600 mg/dl. Mean and the percentage difference between direct and calculated LDL was lowest with the DM approach. Bland–Altman plot shows the best agreement of the DM approach with direct LDL. Conclusion: This study indicates that the DM approach is closer to direct LDL compared to FF & AF.
Journal of Diabetes Mellitus
Background and objectives: Vitamin D is a fat-soluble vitamin belonging to secosteriods. It is re... more Background and objectives: Vitamin D is a fat-soluble vitamin belonging to secosteriods. It is responsible for intestinal absorption and bioavailibity of calcium and phosphorus.Synthesisof vitamin D in response to ultraviolet rays in skin depends on latitude, atmospheric pollution, clothing, skin pigmentation, sunscreen usage, duration and time of exposure to sunlight. Deficiency causes rickets, defective bone mineralization, osteomalacia and various non skeletal diseases such asrheumatoid arthritis, diabetes mellitus, coronary artery disease and cancer. Recent studies have suggested a deficiency state in Indian populationinspite of ample sunshine. Hence, this study was designed to estimate the prevalence of vitamin D deficiency in adults in the coastal regions of Odisha, India Material and methods: This cross-sectional study included 3056 conducted among healthy population of Cuttack, a coastal district of Odisha. All the individuals were tested for serum 25(OH)D level by electroch...
Background and objectives: Prediabetic is the precursor state of the disease Diabetic Mellitus (D... more Background and objectives: Prediabetic is the precursor state of the disease Diabetic Mellitus (DM).It is a metabolic diathesis with high blood sugar and dyslipidaemia. This increases the risk for the development of DM and cardiovascular diseases. The prediabetic state is classified into: (1) the impaired fasting glycemia (IFG) and (2) impaired glucose tolerance (IGT). WHO has defined impaired fasting glucose level as 110 to 125mg% and impaired glucose tolerance as that 2hour plasma glucose to be within the range of 140 to 200 mg% after an oral glucose load of 75grams. Hence, this study was designed to(1) evaluate the role oxidative stress, endothelial dysfunction , inflammation (2) find diagnostic markers for early detection of changes in prediabetes to prevent DM. Material and method: This case control study included 112prediabetic individuals and an equal number of matched healthy volunteers. The FBG, lipid profile, HbA1c and CRP were estimated. We used ferrous oxidation in xylen...
Background and objective: The serum Low density Lipoprotein Cholesterol Concentration can be esti... more Background and objective: The serum Low density Lipoprotein Cholesterol Concentration can be estimated by Freidewald formula and also by the direct assay methods by a spectrophotometer. The National Cholesterol Education program recommends the direct assay for estimating serum LDL-C concentration, but it has not been evaluated whether there is any difference between Freidwald formula & LDL-C direct assay methods in assessing Patients for CVD ( cardio vascular Disease). Methods: This study included the samples of 28,346 patients whose serum triglyceride level was less than 400 mg%. The study was carried in the month of January to May 2018. Fasting samples and non fasting samples were collected for measurement of lipid profile and commercial kits adapted to autoanalyser were used for the direct assay and Friedewald formula was used to calculate the value. Results: Serum LDL-C levels estimated by the direct method and Friedewald formula had a significant positive correlation (r = 0.968...
According to recent findings both X and Y chromosome could have evolved from autosomal ancestors ... more According to recent findings both X and Y chromosome could have evolved from autosomal ancestors about 300 million years ago. In humans, the typical male has a diploid karyotype of 46 chromosomes i.e. 22 pairs of autosomes and XY pair of gonosomes(46,XY).A standard female karyotype is 46 XX.Thus humans are dimorphic: there is one type of humans who are males and the other type is female. The basis of sexual dimorphism in mammals derives from evolution of sex chromosomes (1). Probably a failure occurred in homologous recombination which resulted in the formation of a small area that was not identical to the two participating chromosomes. The presence or absence of this region coincided with a different pattern of development that altered androgen activity resulting in a sex determining role. In all living mammalians to day, this area in Y chromosome appears to retain the regulatory function and therefore described as the sex Determining Region of Y chromosome or SRY gene(2).But sexua...
BACKGROUND AND OBJECTIVES: Renal dysfunction is end organ damage in hypertension. We investigated... more BACKGROUND AND OBJECTIVES: Renal dysfunction is end organ damage in hypertension. We investigated the association of oxidative stress with estimated glomerular filtration rate in hypertension. METHODS: A total of 116 hypertensive individuals (age 47 ± 10.2 years; 68% males; BMI < 25; without diabetes mellitus, CVD) were included. All the hypertensive subjects were albustix negative. Fasting blood glucose, lipid profile, urea and creatinine was estimated. Serum oxidant load was estimated by ferrous oxidation products in xylenol orange version 2(FOX2) and antioxidant power of serum was estimated by ferric reducing capacity, FRAP assay. Data is represented as mean± SD. Data was analysed by unpaired two-tailed students t test, Pearson's correlation and linear regression. The estimated GFR was calculated by simplified modification of diet in renal disease study prediction equation and Cockroft-Gault formula. RESULT: Systolic and diastolic blood pressure, lipid profile, serum creatinine and oxidant load was significantly higher in hypertensive subjects (p < 0.001). Total Antioxidant status and estimated GFR was significantly lower in hypertensive subjects. A significant negative correlation was present between oxidative stress and eGFR. INTERPRETATION AND CONCLUSION: Among hypertensive subjects with preserved renal function increased oxidative stress is associated with decreased eGFR. Whether estimation of oxidative stress and adjuvant antioxidant therapy are better prognostic markers requires further research.
Laboratory Medicine, 2022
Objective Upregulation of matrix metalloproteinase-7 (MMP-7) is associated with hypertension and ... more Objective Upregulation of matrix metalloproteinase-7 (MMP-7) is associated with hypertension and kidney fibrosis, which can progress to chronic kidney disease (CKD). Currently, kidney fibrosis is only detectable by an invasive procedure. Therefore, we set out to determine whether MMP-7 can act as a noninvasive biomarker in patients with hypertension to enable early detection of kidney fibrosis. Materials and Methods Diagnosed patients with hypertension and control patients were sampled. We diagnosed CKD using clinical and laboratory parameters. Serum urea, creatinine, urinary microalbumin, the albumin-to-creatinine ratio, and urinary MMP-7 were analyzed. Results The 195 patients with hypertension had significantly elevated MMP-7. Of these patients, 166 had MMP-7 >25.8 μg/L, whereas only 29 had MMP-7 <25.8 μg/L. Thirty-two patients with hypertension showed features of CKD, all of whom had urinary MMP-7 >25.8 μg/L. However, the urinary MMP-7 level did not differ with the seve...
International Journal of Diabetes in Developing Countries, 2012
ABSTRACT Inflammation, endothelial dysfunction, and oxidative stress are postulated to be the pri... more ABSTRACT Inflammation, endothelial dysfunction, and oxidative stress are postulated to be the principal events in the pathogenesis of type 2 diabetes mellitus and its vascular complications. Serum Nitric Oxide metabolites (NOX) and high sensitivity C-reactive protein (hs-CRP) were measured in eighty non-obese, type 2 diabetic males (40 to 65 years) without (group B, N040) and with vascular disease [16 retinopathy (group C), 24 hypertension (group D)]; and compared with forty healthy, age and sex matched control subjects (group A). The mean age of diabetic patients and healthy controls was 49.5±5.80 vs. 51.0±7.15 years, respectively (P00.212). Diabetic group had higher Fasting Plasma Glucose (FPG), hs-CRP, and NOX levels than control group (135.3±28.69 vs. 96.7±10.46; 5.1±2.59 vs. 1.7± 0.54; 61.1±15.67 vs. 37.1±3.69, respectively, P00.001). When compared with diabetics without complication (53.7±10.37), levels of NOX were significantly higher (P0 0.001) both in retinopathy (74.5±19.39) and hypertension (64.4±13.60) group, whereas that of hs-CRP (4.4±2.85 vs.6.5±2.93, P00.015) differed only in retinopathy group. There was no difference between retinopathy and hypertension group. There was significant (P<0.05) positive correlation between age, duration of diabetes, hs-CRP, and NOX; hs-CRP and NOX among group B and C. A negative correlation was noted between hs-CRP and NOX (r0−0.068, P0 0.752) among hypertensives. By multivariate regression analysis, FPG, hs-CRP and NOX were found to be independent indicators of complications after adjustment for age and duration of diabetes. This study reveals that NOX and hs- CRP are important biomarkers in type 2 diabetes mellitus and its associated vascular complications.
Introduction: Impaired fibrinolysis and low-grade systemic inflammation are postulated to be asso... more Introduction: Impaired fibrinolysis and low-grade systemic inflammation are postulated to be associated with the pathogenesis of type 2 diabetes mellitus (T2DM) with its micro and macrovascular complications. However, this mechanism is complex and poorly understood. Materials and Methods: Fasting plasma glucose (FPG), plasma fibrinogen, serum high-sensitivity C-reactive protein (hs-CRP), and lipid parameters were measured among eighty, nonobese, adult type 2 diabetic males (age: 40–65 years) (without complications [ n = 40, Group 2], with retinopathy [ n = 16, Group 3], with hypertension [ n = 24, Group 4]), and compared with age- and gender-matched healthy controls ( n = 40, Group 1). Results: The mean age of subjects in Groups 1, 2, 3, and 4 were 51 ± 7.2, 49 ± 5.3, 51.5 ± 7.2, and 49.3 ± 5.3 years, respectively ( P = 0.33). Fibrinogen, FPG, and hs-CRP were significantly higher among diabetics than controls ( P P = 0.01) among diabetics with complications than without complication...
Aim and Objective: Omentin-1 is a novel adipokine expressed in visceral adipose tissue and has be... more Aim and Objective: Omentin-1 is a novel adipokine expressed in visceral adipose tissue and has been reported to be negatively associated with insulin resistance (IR). The present study aimed at assessing the correlation of serum Omentin-1 with IR in Type II Diabetes mellitus patients as measured by Homeostasis Model Assessment (HOMA-IR). There has been very few reports (if any) from Indian subcontinent on the role of Omentin in IR. Materials and Methods: It is a case control study, fifty cases of Type II DM patients and equal number of age and sex matched controls were included in the study. Fasting serum Omentin, fasting serum insulin were estimated by ELISA using commercially available ELISA kit and fasting blood sugar by glucose oxidase method by using EM360 autoanalyzer. HOMA-IR score was calculated as HOMA-IR = [Fasting Glucose (mg/dl) × Fasting Insulin (μIU/ml)] / 405. Serum Omentin-1 was correlated with FBS, serum insulin and HOMA-IR using SPSS version-20. Results: Significan...
Biomedicine
Background: Secondary antiphospholipid syndrome (APLS) and rise in antiphospholipid antibody (APL... more Background: Secondary antiphospholipid syndrome (APLS) and rise in antiphospholipid antibody (APLA) has been linked to the development of Disseminated Intravascular Coagulation in COVID-19. But still controversies exist regarding the increase in APLA in COVID -19. Hence, the present study aimed to estimate the levels of APLA in COVID patients and its relationship with the severity of the disease. Materials and Methods: 40 RT PCR positive COVID-19 cases and normal control were recruited for the study. Biochemical and hematological findings were compared in both the groups. COVID-19 patients were further subdivided into survivor Vs non-survivor and based upon the CT findings of thorax they were grouped with vs without CT findings. Results: CRP, PCT, ESR were found to be significantly increased in COVID-19 patients. IgM & IgG APLA antibody (3.02±1.32 U/ml & 3.54±1.85 U/ml) were found to be within normal range in COVID cases. APLA did not show any correlation with serum ferritin, CRP, ...
IOSR Journal of Pharmacy and Biological Sciences, 2012
Background and objectives: Recent studies imply an increasing association of oxidative stress in ... more Background and objectives: Recent studies imply an increasing association of oxidative stress in the pathogenesis of hypertension. This study was designed to evaluate oxidative stress in hypertensive subjects and to assess the correlation of antioxidant status with the severity of hypertension. Material and methods: Ninety-six hypertensive cases were divided into three groups based on severity of hypertension as per JNC VII classification. Lipid profile, antioxidant power of serum(FRAP assay) and oxidant load of serum(FOX2) of hypertensive cases were compared with an equal number of age and sex matched healthy normotensive controls. Data was analysed by Student's t test and Pearson's correlation. Result: Lipid profile, oxidant load was significantly higher in hypertensive patients in comparison to controls (P<0.001). Antioxidant status was lower in hypertensives as compared to controls. A significant correlation was observed between lipid profile vs. oxidative stress and hypertension vs. low antioxidant level (r=0.835, P<0.001) Conclusion: Above findings demonstrate a strong association between blood pressure, dyslipidaemia and oxidative parameters shows a possible role of oxidative stress in the severity of hypertension.
Biomedicine
Background: Secondary antiphospholipid syndrome (APLS) and rise in antiphospholipid antibody (APL... more Background: Secondary antiphospholipid syndrome (APLS) and rise in antiphospholipid antibody (APLA) has been linked to the development of Disseminated Intravascular Coagulation in COVID-19. But still controversies exist regarding the increase in APLA in COVID -19. Hence, the present study aimed to estimate the levels of APLA in COVID patients and its relationship with the severity of the disease. Materials and Methods: 40 RT PCR positive COVID-19 cases and normal control were recruited for the study. Biochemical and hematological findings were compared in both the groups. COVID-19 patients were further subdivided into survivor Vs non-survivor and based upon the CT findings of thorax they were grouped with vs without CT findings. Results: CRP, PCT, ESR were found to be significantly increased in COVID-19 patients. IgM & IgG APLA antibody (3.02±1.32 U/ml & 3.54±1.85 U/ml) were found to be within normal range in COVID cases. APLA did not show any correlation with serum ferritin, CRP, ...
Biomedicine
Introduction and Aim: Serum creatinine (SCr) and estimated glomerular filtration rate (eGFR) give... more Introduction and Aim: Serum creatinine (SCr) and estimated glomerular filtration rate (eGFR) gives the idea of overall functional status of kidney. Measurement of SCr by different method and discordant result between them can misclassify different stages of chronic kidney disease (CKD). Many of the tertiary care health centre, SCr measured by Jaffe method is still the method of choice because of its cost effectiveness even if it is more susceptible for interference. Our aim was to measure SCr level by both Jaffe’s & enzymatic method and analysing the discordance rate of eGFR and their effect in staging of kidney disease in CKD patients. Materials and Methods: In this observational study 330 serum sample were analysed for creatinine by Jaffe’s and enzymatic methods in a fully automated analyser using commercially available kits. Modification of Diet in Renal Disease (MDRD) formula was used for estimating GFR. Results: In 330 subjects eGFR values calculated on incorporating SCr were f...