Stella Th. Girousi - Academia.edu (original) (raw)
Papers by Stella Th. Girousi
This review points out the importance of coupling electrochemical techniques with molecularly imp... more This review points out the importance of coupling electrochemical techniques with molecularly imprinted polymers, in order advanced sensing devices to be developed. Recently, the growing interest in molecularly imprinted polymers (MIPs) as recognition elem receptors, has made the researchers to design novel formats for improvement of MIP electrochemical sensors. Among other possible approaches used in the literature, this review will discuss the electrogeneration of MIPs and on less classical MIPs, or nanotechnology).
This article was originally published in the Encyclopedia of Food and Health published by Elsevie... more This article was originally published in the Encyclopedia of Food and Health published by Elsevier, and the attached copy is provided by Elsevier for the author's benefit and for the benefit of the author's institution, for non-commercial research and educational use including without limitation use in instruction at your institution, sending it to specific colleagues who you know, and providing a copy to your institution's administrator. All other uses, reproduction and distribution, including without limitation commercial reprints, selling or licensing copies or access, or posting on open internet sites, your personal or institution's website or repository, are prohibited. For exceptions, permission may be sought for such use through Elsevier's permissions site at: http://www.elsevier.com/locate/permissionusematerial
ARTICLE INFO ABSTRACT A novel step electropolymerization technique, using the natural dye safrani... more ARTICLE INFO ABSTRACT A novel step electropolymerization technique, using the natural dye safranine nanoparticles (AgNPs). The dsDNA with silver nanoparticles potensiodynamically on the surface of carbon paste electrode (CPE), using cyclic voltammetry (CV The morphology and performance of bioimprinted polymer were characterized by electron scanning microscopy (SEM. The analytical performance of the bioimprinted sensor was also studied. The modified electrode presented very good reproducibility, satisfact sensitivity and selectivity compared to the conventional DNA and electrochemical sensors. Furthermore, the square wave voltammetric peak current was linear to butyl paraben mass concentration in the range 0.362 to 100 bioimprinted sensor was successfully applied to the determination of butyl paraben in real cosmetic samples with satisfactory recovery ranging from 97.3 to 100.2 %, demonstrating its feasibility for practical ap
In this article, the electrochemical behavior of triethanolimine is described with a carbon paste... more In this article, the electrochemical behavior of triethanolimine is described with a carbon paste electrode. A differential pulse voltammetric method was also developed for the determination of triethanolamine as an application to electroanalysis at a carbon paste electrode. The resultant voltammograms have revealed the irreversible electrochemical process that can be utilized to quantify the substances of interest, for triethanolamine, for which the detection limit (3) was estimated to be 2.8 10 −5 molL −1 .
In the present article, the research and development of electrochemical techniques, namely voltam... more In the present article, the research and development of electrochemical techniques, namely voltammetric measurements, applied in the DNA electroanalysis during the past years have been reviewed (in five parts). In the first section, the applications of DNA electrochemistry in the detection of metals are being thoroughly described. The second part gives a critical view in the study of interaction between metals and DNA using electrochemical techniques. Furthermore, attention is paid to the metals and DNA damage detection using selected electrochemical techniques. The following part offers discussion on the detection of DNA sequences / oligonucleotides using metals and electrochemical techniques. Finally, in the last part, the interactions between the antitumor drugs and DNA when using electrochemical techniques are being concerned and critically evaluated.
The research and development of electrochemical biosensors applied to life sciences during the pa... more The research and development of electrochemical biosensors applied to life sciences during the past years were reviewed in the present article. In the first part of the article, a brief introduction on nanomaterial based electrochemical DNA biosensors is given. In the second part DNA biosensors and transition metal complexes with biological interest are described. Finally, the development trends of electrochemical biosensors in genetic and epigenetic testing are being discussed.
and sharing with colleagues.
Analytical Chemistry Insights, 2016
Investigation of the electrochemical behavior using cyclic voltammetry and detection of [Mn 2+ (t... more Investigation of the electrochemical behavior using cyclic voltammetry and detection of [Mn 2+ (thiophenyl-2-carboxylic acid) 2 (triethanolamine)] with adsorptive stripping differential pulse voltammetry. The electrochemical behavior of a manganese(II) complex [Mn 2+ (thiophenyl-2-carboxylic acid) 2 (triethanolamine)] (A) was investigated using cyclic and differential pulse voltammetry in an acetate buffer of pH 4.6 at a carbon paste electrode. Further, an oxidation-reduction mechanism was proposed. Meanwhile, an adsorptive stripping differential pulse voltammetric method was developed for the determination of manganese(II) complex.
Abstract: In the field of electrochemical biosensing, transition metal complexes achieved a signi... more Abstract: In the field of electrochemical biosensing, transition metal complexes achieved a significant importance as hybridization indicators or electroactive markers of DNA. Their incorporation in electro-chemical DNA biosensors enables to offer a promising perspective in understanding of the biological activity of some chemical compounds. In this context, the development of innovative configurations of electrochemical DNA biosensors applied to life sciences during the last years were reviewed in the present article. In the first part, a brief introduction of nanomaterial based electrochemical DNA biosensors is given. In the second part, the complexes of transition metals with biological interest and their applications in electrochemical DNA biosensors are being described.
A novel, sensitive and selective electrochemical hybridisation biosensor was developed for the de... more A novel, sensitive and selective electrochemical hybridisation biosensor was developed for the detection of the hepatitis B virus (HBV) using a manganese(II) complex as electrochemical indicator and a DNA probe-modified carbon paste electrode as the biosensor (DNA/CPE). The results showed that this complex could be accumulated electrochemically the immobilised dsDNA layer rather than in the single-stranded DNA (ssDNA) layer. On the basis of this, the manganese complex was used as an electrochemical hybridisation indicator for the detection of oligonucleotides related to HBV. The hybridisation event was evaluated on the basis of the difference between the reduction signals of the manganese(II) complex with the probe DNA prior to and post hybridisation with a target sequence using a differential pulse mode. Several factors affecting the immobilisation and hybridisation of oligonucleotides as well as the indicator's accumulation were investigated. Experiments with a noncomplementary and mismatch sequences demonstrated the good selectivity of the biosensor. Using this approach, the HBV target oligonucleotide's sequence could be quantified over arange from 0.22 ng L −1 to 5.40 ng L −1 , with a linear correlation coefficient of 0.9994 and the limit of detection of 0.07 ng L −1 .
Fresenius' Journal of Analytical Chemistry, 1997
The user has requested enhancement of the downloaded file. All in-text references underlined in b... more The user has requested enhancement of the downloaded file. All in-text references underlined in blue are added to the original document and are linked to publications on ResearchGate, letting you access and read them immediately.
International Journal of Environmental Analytical Chemistry, 2003
2009 2nd International Symposium on Applied Sciences in Biomedical and Communication Technologies, 2009
An electrochemical DNA hybridization biosensor was developed for the detection of DNA hybridizati... more An electrochemical DNA hybridization biosensor was developed for the detection of DNA hybridization using MDB and proflavine as electrochemical labels. The biosensor was based on the interaction of 7-dimethyl-amino-1,2-benzophenoxazinium Meldola's Blue (MDB) and proflavine also, with double stranded DNA (dsDNA) The electrochemical behaviour of MDB and proflavine as well its interaction with double stranded (dsDNA) were investigated by cyclic (CV)
The mononuclear complex [Mn(thiophenyl-2-carboxylate) 2 (H 3 tea)] (1), where H 3 tea=triethanola... more The mononuclear complex [Mn(thiophenyl-2-carboxylate) 2 (H 3 tea)] (1), where H 3 tea=triethanolamine has been synthesized, characterized, while the DNA binding properties and the antioxidant activity were studied. The crystal structure of 1 is also reported. The interaction between 1 and calf thymus double stranded (ds) DNA was electrochemically at a calf thymus DNA (CT-DNA) modified carbon paste electrode (CPE) and spectrophotometrically investigated, respectively. Adsorptive transfer stripping voltammetry showed that the interaction mode between 1 and CT-DNA depends on manganese(II) complex concentration. UV studies between 1 and CT-DNA revealed that 1 can bind to CT-DNA by the intercalative binding mode and the binding constant has been calculated. A competitive study with acridine orange (AO) showed that 1 exhibits the ability to displace the DNA-bound AO, since 1 binds to the DNA in competition with AO. In vitro antioxidant activity of free ligands and 1 was evaluated using five different antioxidant assays: a) interaction with 1, 1-diphenyl-2-picryl-hydrazyl (DPPH) stable free radical, b) the ΗΟ• mediated oxidation of DMSO, c) scavenging of superoxide anion radicals, d) inhibition of lipid peroxidation and e) soybean lipoxygenase (LOX) inhibition. The results revealed the selectivity of the manganese complex to different free radicals as a consequence of its physicochemical feature. In particular, 1 presents significant inhibitory activity on LOX, with IC 50 =30 μM and selectivity to the inhibition of superoxide anion radicals and thus a promising candidate as a superoxide dismutase (SOD) biomimetic.
The interaction of actinomycin (ACTD) with double stranded (ds) calf thymus DNA and single strand... more The interaction of actinomycin (ACTD) with double stranded (ds) calf thymus DNA and single stranded (ss) DNA was studied at the carbon paste electrode surface by means of transfer voltammetry in 0.2 M phosphate buffer solution (pH 7.4). Accordingly the interaction of actinomycin (ACTD) with ds calf thymus DNA, ss DNA and supercoiled (sc) DNA was studied using hanging mercury drop electrode in 0.3 M NaCl, and 50 mM sodium phosphate buffer (pH 8.5). The different electrochemical behaviours are presented and compared in the article. #
International Journal of Environmental Analytical Chemistry, 2004
Actinomycin D is an antitumor antibiotic which intercalates within DNA. The interaction of actino... more Actinomycin D is an antitumor antibiotic which intercalates within DNA. The interaction of actinomycin D with double-stranded and thermally denatured (single-stranded) calf thymus DNA was studied in solution as well as at the electrode surface using differential pulse transfer voltammetry with carbon paste electrode in 0.2 M acetate buffer, pH 5.0.
Electroanalysis, 2006
An electrochemical DNA biosensor was developed by DNA immobilization at the electrode surface and... more An electrochemical DNA biosensor was developed by DNA immobilization at the electrode surface and its electrochemical behavior was studied in relation with different materials added in the paste. The aim was to study new materials for the development of new electrode surfaces, to be applied in the study of DNA -drug interactions. New electrochemical sensing materials using polymer multilayers were reported for the adsorption of DNA. These materials were prepared by mixing a polymer ion exchanger and graphite powder. The mixture was then used to render the modified carbon paste electrode (CPE), on the surface of which the dsDNA was adsorbed and studied by differential pulse voltammetry (DP voltammetry). The signal of guanine oxidation peak of DNA was followed. This modified biosensor was applied for the study of the interaction between DNA and the known intercalators Ethidium Bromide (EB) and Acridine Orange (AO). The established biosensor exhibited an improvement of its sensitivity and repeatability compared with the conventional CPE DNA biosensor.
This review points out the importance of coupling electrochemical techniques with molecularly imp... more This review points out the importance of coupling electrochemical techniques with molecularly imprinted polymers, in order advanced sensing devices to be developed. Recently, the growing interest in molecularly imprinted polymers (MIPs) as recognition elem receptors, has made the researchers to design novel formats for improvement of MIP electrochemical sensors. Among other possible approaches used in the literature, this review will discuss the electrogeneration of MIPs and on less classical MIPs, or nanotechnology).
This article was originally published in the Encyclopedia of Food and Health published by Elsevie... more This article was originally published in the Encyclopedia of Food and Health published by Elsevier, and the attached copy is provided by Elsevier for the author's benefit and for the benefit of the author's institution, for non-commercial research and educational use including without limitation use in instruction at your institution, sending it to specific colleagues who you know, and providing a copy to your institution's administrator. All other uses, reproduction and distribution, including without limitation commercial reprints, selling or licensing copies or access, or posting on open internet sites, your personal or institution's website or repository, are prohibited. For exceptions, permission may be sought for such use through Elsevier's permissions site at: http://www.elsevier.com/locate/permissionusematerial
ARTICLE INFO ABSTRACT A novel step electropolymerization technique, using the natural dye safrani... more ARTICLE INFO ABSTRACT A novel step electropolymerization technique, using the natural dye safranine nanoparticles (AgNPs). The dsDNA with silver nanoparticles potensiodynamically on the surface of carbon paste electrode (CPE), using cyclic voltammetry (CV The morphology and performance of bioimprinted polymer were characterized by electron scanning microscopy (SEM. The analytical performance of the bioimprinted sensor was also studied. The modified electrode presented very good reproducibility, satisfact sensitivity and selectivity compared to the conventional DNA and electrochemical sensors. Furthermore, the square wave voltammetric peak current was linear to butyl paraben mass concentration in the range 0.362 to 100 bioimprinted sensor was successfully applied to the determination of butyl paraben in real cosmetic samples with satisfactory recovery ranging from 97.3 to 100.2 %, demonstrating its feasibility for practical ap
In this article, the electrochemical behavior of triethanolimine is described with a carbon paste... more In this article, the electrochemical behavior of triethanolimine is described with a carbon paste electrode. A differential pulse voltammetric method was also developed for the determination of triethanolamine as an application to electroanalysis at a carbon paste electrode. The resultant voltammograms have revealed the irreversible electrochemical process that can be utilized to quantify the substances of interest, for triethanolamine, for which the detection limit (3) was estimated to be 2.8 10 −5 molL −1 .
In the present article, the research and development of electrochemical techniques, namely voltam... more In the present article, the research and development of electrochemical techniques, namely voltammetric measurements, applied in the DNA electroanalysis during the past years have been reviewed (in five parts). In the first section, the applications of DNA electrochemistry in the detection of metals are being thoroughly described. The second part gives a critical view in the study of interaction between metals and DNA using electrochemical techniques. Furthermore, attention is paid to the metals and DNA damage detection using selected electrochemical techniques. The following part offers discussion on the detection of DNA sequences / oligonucleotides using metals and electrochemical techniques. Finally, in the last part, the interactions between the antitumor drugs and DNA when using electrochemical techniques are being concerned and critically evaluated.
The research and development of electrochemical biosensors applied to life sciences during the pa... more The research and development of electrochemical biosensors applied to life sciences during the past years were reviewed in the present article. In the first part of the article, a brief introduction on nanomaterial based electrochemical DNA biosensors is given. In the second part DNA biosensors and transition metal complexes with biological interest are described. Finally, the development trends of electrochemical biosensors in genetic and epigenetic testing are being discussed.
and sharing with colleagues.
Analytical Chemistry Insights, 2016
Investigation of the electrochemical behavior using cyclic voltammetry and detection of [Mn 2+ (t... more Investigation of the electrochemical behavior using cyclic voltammetry and detection of [Mn 2+ (thiophenyl-2-carboxylic acid) 2 (triethanolamine)] with adsorptive stripping differential pulse voltammetry. The electrochemical behavior of a manganese(II) complex [Mn 2+ (thiophenyl-2-carboxylic acid) 2 (triethanolamine)] (A) was investigated using cyclic and differential pulse voltammetry in an acetate buffer of pH 4.6 at a carbon paste electrode. Further, an oxidation-reduction mechanism was proposed. Meanwhile, an adsorptive stripping differential pulse voltammetric method was developed for the determination of manganese(II) complex.
Abstract: In the field of electrochemical biosensing, transition metal complexes achieved a signi... more Abstract: In the field of electrochemical biosensing, transition metal complexes achieved a significant importance as hybridization indicators or electroactive markers of DNA. Their incorporation in electro-chemical DNA biosensors enables to offer a promising perspective in understanding of the biological activity of some chemical compounds. In this context, the development of innovative configurations of electrochemical DNA biosensors applied to life sciences during the last years were reviewed in the present article. In the first part, a brief introduction of nanomaterial based electrochemical DNA biosensors is given. In the second part, the complexes of transition metals with biological interest and their applications in electrochemical DNA biosensors are being described.
A novel, sensitive and selective electrochemical hybridisation biosensor was developed for the de... more A novel, sensitive and selective electrochemical hybridisation biosensor was developed for the detection of the hepatitis B virus (HBV) using a manganese(II) complex as electrochemical indicator and a DNA probe-modified carbon paste electrode as the biosensor (DNA/CPE). The results showed that this complex could be accumulated electrochemically the immobilised dsDNA layer rather than in the single-stranded DNA (ssDNA) layer. On the basis of this, the manganese complex was used as an electrochemical hybridisation indicator for the detection of oligonucleotides related to HBV. The hybridisation event was evaluated on the basis of the difference between the reduction signals of the manganese(II) complex with the probe DNA prior to and post hybridisation with a target sequence using a differential pulse mode. Several factors affecting the immobilisation and hybridisation of oligonucleotides as well as the indicator's accumulation were investigated. Experiments with a noncomplementary and mismatch sequences demonstrated the good selectivity of the biosensor. Using this approach, the HBV target oligonucleotide's sequence could be quantified over arange from 0.22 ng L −1 to 5.40 ng L −1 , with a linear correlation coefficient of 0.9994 and the limit of detection of 0.07 ng L −1 .
Fresenius' Journal of Analytical Chemistry, 1997
The user has requested enhancement of the downloaded file. All in-text references underlined in b... more The user has requested enhancement of the downloaded file. All in-text references underlined in blue are added to the original document and are linked to publications on ResearchGate, letting you access and read them immediately.
International Journal of Environmental Analytical Chemistry, 2003
2009 2nd International Symposium on Applied Sciences in Biomedical and Communication Technologies, 2009
An electrochemical DNA hybridization biosensor was developed for the detection of DNA hybridizati... more An electrochemical DNA hybridization biosensor was developed for the detection of DNA hybridization using MDB and proflavine as electrochemical labels. The biosensor was based on the interaction of 7-dimethyl-amino-1,2-benzophenoxazinium Meldola's Blue (MDB) and proflavine also, with double stranded DNA (dsDNA) The electrochemical behaviour of MDB and proflavine as well its interaction with double stranded (dsDNA) were investigated by cyclic (CV)
The mononuclear complex [Mn(thiophenyl-2-carboxylate) 2 (H 3 tea)] (1), where H 3 tea=triethanola... more The mononuclear complex [Mn(thiophenyl-2-carboxylate) 2 (H 3 tea)] (1), where H 3 tea=triethanolamine has been synthesized, characterized, while the DNA binding properties and the antioxidant activity were studied. The crystal structure of 1 is also reported. The interaction between 1 and calf thymus double stranded (ds) DNA was electrochemically at a calf thymus DNA (CT-DNA) modified carbon paste electrode (CPE) and spectrophotometrically investigated, respectively. Adsorptive transfer stripping voltammetry showed that the interaction mode between 1 and CT-DNA depends on manganese(II) complex concentration. UV studies between 1 and CT-DNA revealed that 1 can bind to CT-DNA by the intercalative binding mode and the binding constant has been calculated. A competitive study with acridine orange (AO) showed that 1 exhibits the ability to displace the DNA-bound AO, since 1 binds to the DNA in competition with AO. In vitro antioxidant activity of free ligands and 1 was evaluated using five different antioxidant assays: a) interaction with 1, 1-diphenyl-2-picryl-hydrazyl (DPPH) stable free radical, b) the ΗΟ• mediated oxidation of DMSO, c) scavenging of superoxide anion radicals, d) inhibition of lipid peroxidation and e) soybean lipoxygenase (LOX) inhibition. The results revealed the selectivity of the manganese complex to different free radicals as a consequence of its physicochemical feature. In particular, 1 presents significant inhibitory activity on LOX, with IC 50 =30 μM and selectivity to the inhibition of superoxide anion radicals and thus a promising candidate as a superoxide dismutase (SOD) biomimetic.
The interaction of actinomycin (ACTD) with double stranded (ds) calf thymus DNA and single strand... more The interaction of actinomycin (ACTD) with double stranded (ds) calf thymus DNA and single stranded (ss) DNA was studied at the carbon paste electrode surface by means of transfer voltammetry in 0.2 M phosphate buffer solution (pH 7.4). Accordingly the interaction of actinomycin (ACTD) with ds calf thymus DNA, ss DNA and supercoiled (sc) DNA was studied using hanging mercury drop electrode in 0.3 M NaCl, and 50 mM sodium phosphate buffer (pH 8.5). The different electrochemical behaviours are presented and compared in the article. #
International Journal of Environmental Analytical Chemistry, 2004
Actinomycin D is an antitumor antibiotic which intercalates within DNA. The interaction of actino... more Actinomycin D is an antitumor antibiotic which intercalates within DNA. The interaction of actinomycin D with double-stranded and thermally denatured (single-stranded) calf thymus DNA was studied in solution as well as at the electrode surface using differential pulse transfer voltammetry with carbon paste electrode in 0.2 M acetate buffer, pH 5.0.
Electroanalysis, 2006
An electrochemical DNA biosensor was developed by DNA immobilization at the electrode surface and... more An electrochemical DNA biosensor was developed by DNA immobilization at the electrode surface and its electrochemical behavior was studied in relation with different materials added in the paste. The aim was to study new materials for the development of new electrode surfaces, to be applied in the study of DNA -drug interactions. New electrochemical sensing materials using polymer multilayers were reported for the adsorption of DNA. These materials were prepared by mixing a polymer ion exchanger and graphite powder. The mixture was then used to render the modified carbon paste electrode (CPE), on the surface of which the dsDNA was adsorbed and studied by differential pulse voltammetry (DP voltammetry). The signal of guanine oxidation peak of DNA was followed. This modified biosensor was applied for the study of the interaction between DNA and the known intercalators Ethidium Bromide (EB) and Acridine Orange (AO). The established biosensor exhibited an improvement of its sensitivity and repeatability compared with the conventional CPE DNA biosensor.