Stig Djurhuus - Academia.edu (original) (raw)
Papers by Stig Djurhuus
Diabetologia, 1993
The absorption rate of rapid acting (soluble) insulin is slow from the subcutaneous tissue of the... more The absorption rate of rapid acting (soluble) insulin is slow from the subcutaneous tissue of the thigh compared to intramuscular injection into the thigh and s. c. injection into the abdominal wall. The aim of the study was to evaluate the impact of soluble insulin injected either intramuscularly into the thigh (IMT), s. c. into the abdominal wall (SCA) or s.c. into the thigh (SCT) on glycaemic control in Type 1 (insulin-dependent) diabetic outpatients treated with the basal bolus insulin delivery regimen. Fifty-five, C-peptide negative Type 1 diabetic outpatients were included in a randomised 3-month intervention study. The insulin doses were adjusted frequently by blinded observers based on the patients' self-monitored blood glucose values and reported hypoglycaemic episodes. The serum fructosamine value was within normal limits in three patients in the IMT group, in six patients in the SCA group and in none of the patients in the SCT group following the intervention period (p < 0.01). However, the difference in mean serum fructosamine values did not reach statistical significance (IMT: 1.24 retool/1 (95 % confidence interval; 1.17 to 1.31), SCA: 1.25 mmol/1 (1.18 to 1.32), SCT: 1.34 mmol/1 (1.26 to 1.41), (p = 0.09)). Blood glucose excursions were larger in the SCT group than in the SCA and IMT group from post-lunch to pre-dinner measurements and from pre-to post-dinner measurements. A higher number of measured low nocturnal blood glucose values (less than 4 mmol/1) was observed in the SCT group (34 of 85) than in the IMT (14 of 64) and SCA (21 of 81) group (p < 0.05). Three patients in the IMT group, two in the SCA group, and seven in the SCT group experienced severe hypoglycaemic episodes (p = 0.14). In conclusion s. c. injection of soluble insulin into the abdominal wall is preferable compared to s. c. injection into the thigh in the basal bolus insulin delivery regimen. Furthermore, soluble insulin injection s. c. into the thigh during daytime has important clinical implications for the development of nocturnal hypoglycaemia independently of the NPH insulin injection at bedtime.
Ugeskrift For Laeger, May 1, 2008
A 66 year-old man presented drug-refractory, repetitive ventricular tachyarrhythmias after acute ... more A 66 year-old man presented drug-refractory, repetitive ventricular tachyarrhythmias after acute myocardial infarction. Despite successful revascularization, the patient suffered recurrent episodes of rapid ventricular tachycardia which could only be managed by radiofrequency ablation.
Diabetic Medicine a Journal of the British Diabetic Association, Jun 1, 1991
The absorption kinetics of NPH (isophane) insulin injected subcutaneously into the abdominal wall... more The absorption kinetics of NPH (isophane) insulin injected subcutaneously into the abdominal wall and subcutaneously (SC) and intramuscularly (IM) into the thigh was studied in 11 Type 1 diabetic patients. The thickness of the subcutaneous adipose tissue layer was measured by ultrasound. NPH (isophane) insulin injected IM into the thigh was absorbed faster than NPH insulin injected SC into the thigh (T50%, IM 8.0 +/- 0.6 h and SC 10.3 +/- 0.7 h, p less than 0.05). No difference in T50% values was found for injection into the abdominal wall (9.7 +/- 1.2h) compared with the thigh. The mean absorption rate from 1.5 to 13.5 h after injection was higher after injection IM into the thigh (6.4 +/- 0.3% of initial dose injected absorbed per h) than after SC injection into the thigh (5.2 +/- 0.3% h-1) and SC into the abdominal wall (5.1 +/- 0.3% h-1) (p less than 0.01). The most constant absorption rate was obtained after SC injection into the thigh (within-study day CV of the mean absorption rate 19.9 +/- 3.2% vs 34.4 +/- 3.2% after IM injection into the thigh and 27.1 +/- 4.9% after SC injection into the abdominal wall (p less than 0.02]. The study provides further evidence that the subcutaneous tissue of the thigh is the preferred injection site for NPH insulin.
Ugeskrift For Laeger, Apr 7, 2008
American Journal of Physiology Endocrinology and Metabolism, Dec 1, 1996
CITATIONS 41 READS 55 4 authors: Some of the authors of this publication are also working on thes... more CITATIONS 41 READS 55 4 authors: Some of the authors of this publication are also working on these related projects: No longer active with research View project Calcium content and respiratory control index of skeletal muscle mitochondria during exercise and recovery. Am. J. Physiol. 271 (Endocrinol.
J Clin Endocrinol Metab, 2001
A reduced functional capacity of the sodium (Na), potassium (K) pump might reduce energy expendit... more A reduced functional capacity of the sodium (Na), potassium (K) pump might reduce energy expenditure, inducing obesity and type 2 diabetes. Consequently, the Na and K content and [ 3 H]ouabain binding capacity of skeletal muscle were measured in 10 monozygotic twin pairs discordant for type 2 diabetes and in 10 obese controls. Muscle [ 3 H]ouabain binding capacity was reduced by approximately 20% in type 2 diabetes. Removing the genetic component by looking at differences within twin pairs, the difference in waist/hip ratio was associated with the difference in [ 3 H]ouabain binding (r ϭ Ϫ0.85; P Ͻ 0.002). Except for the type 2 diabetic twins in the basal state, both basal and insulin-stimulated energy expenditure were associated with the muscle K/Na ratio in the twins. In controls, the 2-h plasma glucose concentration during an oral glucose tolerance test was associated with the change in both muscle and plasma K induced by a euglycemic, hyperinsulinemic clamp. In conclusion, environmental factors related to the waist/hip ratio reduce the muscle [ 3 H]ouabain binding capacity in type 2 diabetes. Without proving causality, the muscle K/Na ratio is associated with energy expenditure in individuals genetically predisposed to the development of type 2 diabetes.
Ugeskrift For Laeger, 2008
Journal of Applied Physiology
The effects of 4 wk of detraining on maximal O2 uptake (VO2max) and on endurance capacity defined... more The effects of 4 wk of detraining on maximal O2 uptake (VO2max) and on endurance capacity defined as the maximal time to exhaustion at 75% of VO2max were studied in nine well-trained endurance athletes. Detraining consisted of one short 35-min high-intensity bout per week as opposed to the normal 6-10 h/wk. Detraining had no effect on VO2max (4.57 +/- 0.10 vs. 4.54 +/- 0.08 l/min), but endurance capacity decreased by 21% from 79 +/- 4 to 62 +/- 4 min (P < 0.001). Endurance exercise respiratory exchange ratio was higher in the detrained than in the trained state (0.91 +/- 0.01 vs. 0.89 +/- 0.01; P < 0.01). Muscle [K+] values were unchanged during exercise and were similar in the trained and detrained states. Muscle [Mg2+] values were similar at rest and at minute 40 (30.3 +/- 0.9 vs. 30.8 +/- 0.6 mmol/kg dry wt) but increased significantly at exhaustion to 33.8 +/- 1.0 mmol/kg dry wt in the trained state and to 33.9 +/- 0.9 mmol/kg dry wt in the detrained state. The elevated muscle [Mg2+] at exhaustion could contribute to fatigue in prolonged exercise through an inhibition of Ca2+ release from sarcoplasmic reticulum. It is concluded that the endurance capacity can vary considerably during detraining without changes in VO2max. Altered substrate utilization or changes in electrolyte regulation may account for the reduced endurance capacity.
Ugeskrift for laeger
The attitude towards the treatment of acute myocardial infarction is currently changing. As an ex... more The attitude towards the treatment of acute myocardial infarction is currently changing. As an example of one of the new methods, a successful case of acute percutaneous transluminal coronary angioplasty is presented. Which of the new therapeutic regimens provides the best prognosis is unknown, but considering the resources and the hitherto published studies, intravenous thrombolysis seems in general to be the most advantageous, while the others ought only to be used on special indications.
Ugeskrift for laeger, Jan 7, 2008
A 66 year-old man presented drug-refractory, repetitive ventricular tachyarrhythmias after acute ... more A 66 year-old man presented drug-refractory, repetitive ventricular tachyarrhythmias after acute myocardial infarction. Despite successful revascularization, the patient suffered recurrent episodes of rapid ventricular tachycardia which could only be managed by radiofrequency ablation.
Upsala Journal of Medical Sciences, 1993
In order to investigate the relevance of the currently used lower reference limit for S-Potassium... more In order to investigate the relevance of the currently used lower reference limit for S-Potassium in Danish hospital laboratories, analytical bias in the measurement of S-Potassium was compared with the lower reference limit in each of 52 Danish hospital ...
Medicine& Science in Sports & Exercise, 1998
![Research paper thumbnail of Muscle Sodium, Potassium, and[ 3 H]Ouabain Binding in Identical Twins, Discordant for Type 2 Diabetes 1](https://attachments.academia-assets.com/51178081/thumbnails/1.jpg)
](The Journal of Clinical Endocrinology & Metabolism, 2001
A reduced functional capacity of the sodium (Na), potassium (K) pump might reduce energy expendit... more A reduced functional capacity of the sodium (Na), potassium (K) pump might reduce energy expenditure, inducing obesity and type 2 diabetes. Consequently, the Na and K content and [ 3 H]ouabain binding capacity of skeletal muscle were measured in 10 monozygotic twin pairs discordant for type 2 diabetes and in 10 obese controls. Muscle [ 3 H]ouabain binding capacity was reduced by approximately 20% in type 2 diabetes. Removing the genetic component by looking at differences within twin pairs, the difference in waist/hip ratio was associated with the difference in [ 3 H]ouabain binding (r ϭ Ϫ0.85; P Ͻ 0.002). Except for the type 2 diabetic twins in the basal state, both basal and insulin-stimulated energy expenditure were associated with the muscle K/Na ratio in the twins. In controls, the 2-h plasma glucose concentration during an oral glucose tolerance test was associated with the change in both muscle and plasma K induced by a euglycemic, hyperinsulinemic clamp. In conclusion, environmental factors related to the waist/hip ratio reduce the muscle [ 3 H]ouabain binding capacity in type 2 diabetes. Without proving causality, the muscle K/Na ratio is associated with energy expenditure in individuals genetically predisposed to the development of type 2 diabetes.
International Journal of Cardiology, 2004
ABSTRACT New non-pharmacological interventions have evolved over the latest decades in order to p... more ABSTRACT New non-pharmacological interventions have evolved over the latest decades in order to prevent atrial fibrillation and/or reduce symptoms. Radiofrequency ablation (RFA) has been performed in Denmark since 2001. In 2011, 2,529 catheter-based RFAs for atrial fibrillation were performed. RFA is now considered a treatment option in patients with drug-refractory atrial fibrillation or young patients who prefer RFA rather than anti-arrhythmic drugs. RFA aims at minimising the impact of "trigger foci" from the pulmonary veins or inhibiting the evolvement of electrical impulses within the atrium.
Analytical Biochemistry, 1998
The aim of the study was to evaluate the use of freeze-dried and dissected small muscle biopsy sp... more The aim of the study was to evaluate the use of freeze-dried and dissected small muscle biopsy specimens ("dry") for the determination of human muscle electrolyte content and ouabain binding capacity, compared with an easier method, without this freezedrying step ("wet"). Freeze-drying and dissection of muscle biopsy specimens reduced the variation in the determination of muscle potassium and magnesium content. The total coefficient of variation was 8.6% in the dry determination of muscle potassium content and 13.5% in the wet determination (P < 0.05). In the determination of muscle magnesium content, the total coefficient of variation was 7.4% in the dry determination and 13.7% when determined wet (P < 0.005). Muscle sodium content had a very large coefficient of variation, independent of the method used. The content of dry solids was too high in biopsies which were incubated in Tris-vanadate buffer (31.9%), compared to biopsies which were not incubated in Tris-vanadate buffer (24.9%, P < 0.001). Hereby, the measured ouabain binding capacity became too high when measured wet. In conclusion, muscle electrolyte content and ouabain binding capacity should be determined after drying and microdissection of the biopsies, because this method confers the least variation and the highest accuracy.
Ugeskrift For Laeger, 2013
Diabetologia, 1993
The absorption rate of rapid acting (soluble) insulin is slow from the subcutaneous tissue of the... more The absorption rate of rapid acting (soluble) insulin is slow from the subcutaneous tissue of the thigh compared to intramuscular injection into the thigh and s. c. injection into the abdominal wall. The aim of the study was to evaluate the impact of soluble insulin injected either intramuscularly into the thigh (IMT), s. c. into the abdominal wall (SCA) or s.c. into the thigh (SCT) on glycaemic control in Type 1 (insulin-dependent) diabetic outpatients treated with the basal bolus insulin delivery regimen. Fifty-five, C-peptide negative Type 1 diabetic outpatients were included in a randomised 3-month intervention study. The insulin doses were adjusted frequently by blinded observers based on the patients' self-monitored blood glucose values and reported hypoglycaemic episodes. The serum fructosamine value was within normal limits in three patients in the IMT group, in six patients in the SCA group and in none of the patients in the SCT group following the intervention period (p < 0.01). However, the difference in mean serum fructosamine values did not reach statistical significance (IMT: 1.24 retool/1 (95 % confidence interval; 1.17 to 1.31), SCA: 1.25 mmol/1 (1.18 to 1.32), SCT: 1.34 mmol/1 (1.26 to 1.41), (p = 0.09)). Blood glucose excursions were larger in the SCT group than in the SCA and IMT group from post-lunch to pre-dinner measurements and from pre-to post-dinner measurements. A higher number of measured low nocturnal blood glucose values (less than 4 mmol/1) was observed in the SCT group (34 of 85) than in the IMT (14 of 64) and SCA (21 of 81) group (p < 0.05). Three patients in the IMT group, two in the SCA group, and seven in the SCT group experienced severe hypoglycaemic episodes (p = 0.14). In conclusion s. c. injection of soluble insulin into the abdominal wall is preferable compared to s. c. injection into the thigh in the basal bolus insulin delivery regimen. Furthermore, soluble insulin injection s. c. into the thigh during daytime has important clinical implications for the development of nocturnal hypoglycaemia independently of the NPH insulin injection at bedtime.
Ugeskrift For Laeger, May 1, 2008
A 66 year-old man presented drug-refractory, repetitive ventricular tachyarrhythmias after acute ... more A 66 year-old man presented drug-refractory, repetitive ventricular tachyarrhythmias after acute myocardial infarction. Despite successful revascularization, the patient suffered recurrent episodes of rapid ventricular tachycardia which could only be managed by radiofrequency ablation.
Diabetic Medicine a Journal of the British Diabetic Association, Jun 1, 1991
The absorption kinetics of NPH (isophane) insulin injected subcutaneously into the abdominal wall... more The absorption kinetics of NPH (isophane) insulin injected subcutaneously into the abdominal wall and subcutaneously (SC) and intramuscularly (IM) into the thigh was studied in 11 Type 1 diabetic patients. The thickness of the subcutaneous adipose tissue layer was measured by ultrasound. NPH (isophane) insulin injected IM into the thigh was absorbed faster than NPH insulin injected SC into the thigh (T50%, IM 8.0 +/- 0.6 h and SC 10.3 +/- 0.7 h, p less than 0.05). No difference in T50% values was found for injection into the abdominal wall (9.7 +/- 1.2h) compared with the thigh. The mean absorption rate from 1.5 to 13.5 h after injection was higher after injection IM into the thigh (6.4 +/- 0.3% of initial dose injected absorbed per h) than after SC injection into the thigh (5.2 +/- 0.3% h-1) and SC into the abdominal wall (5.1 +/- 0.3% h-1) (p less than 0.01). The most constant absorption rate was obtained after SC injection into the thigh (within-study day CV of the mean absorption rate 19.9 +/- 3.2% vs 34.4 +/- 3.2% after IM injection into the thigh and 27.1 +/- 4.9% after SC injection into the abdominal wall (p less than 0.02]. The study provides further evidence that the subcutaneous tissue of the thigh is the preferred injection site for NPH insulin.
Ugeskrift For Laeger, Apr 7, 2008
American Journal of Physiology Endocrinology and Metabolism, Dec 1, 1996
CITATIONS 41 READS 55 4 authors: Some of the authors of this publication are also working on thes... more CITATIONS 41 READS 55 4 authors: Some of the authors of this publication are also working on these related projects: No longer active with research View project Calcium content and respiratory control index of skeletal muscle mitochondria during exercise and recovery. Am. J. Physiol. 271 (Endocrinol.
J Clin Endocrinol Metab, 2001
A reduced functional capacity of the sodium (Na), potassium (K) pump might reduce energy expendit... more A reduced functional capacity of the sodium (Na), potassium (K) pump might reduce energy expenditure, inducing obesity and type 2 diabetes. Consequently, the Na and K content and [ 3 H]ouabain binding capacity of skeletal muscle were measured in 10 monozygotic twin pairs discordant for type 2 diabetes and in 10 obese controls. Muscle [ 3 H]ouabain binding capacity was reduced by approximately 20% in type 2 diabetes. Removing the genetic component by looking at differences within twin pairs, the difference in waist/hip ratio was associated with the difference in [ 3 H]ouabain binding (r ϭ Ϫ0.85; P Ͻ 0.002). Except for the type 2 diabetic twins in the basal state, both basal and insulin-stimulated energy expenditure were associated with the muscle K/Na ratio in the twins. In controls, the 2-h plasma glucose concentration during an oral glucose tolerance test was associated with the change in both muscle and plasma K induced by a euglycemic, hyperinsulinemic clamp. In conclusion, environmental factors related to the waist/hip ratio reduce the muscle [ 3 H]ouabain binding capacity in type 2 diabetes. Without proving causality, the muscle K/Na ratio is associated with energy expenditure in individuals genetically predisposed to the development of type 2 diabetes.
Ugeskrift For Laeger, 2008
Journal of Applied Physiology
The effects of 4 wk of detraining on maximal O2 uptake (VO2max) and on endurance capacity defined... more The effects of 4 wk of detraining on maximal O2 uptake (VO2max) and on endurance capacity defined as the maximal time to exhaustion at 75% of VO2max were studied in nine well-trained endurance athletes. Detraining consisted of one short 35-min high-intensity bout per week as opposed to the normal 6-10 h/wk. Detraining had no effect on VO2max (4.57 +/- 0.10 vs. 4.54 +/- 0.08 l/min), but endurance capacity decreased by 21% from 79 +/- 4 to 62 +/- 4 min (P < 0.001). Endurance exercise respiratory exchange ratio was higher in the detrained than in the trained state (0.91 +/- 0.01 vs. 0.89 +/- 0.01; P < 0.01). Muscle [K+] values were unchanged during exercise and were similar in the trained and detrained states. Muscle [Mg2+] values were similar at rest and at minute 40 (30.3 +/- 0.9 vs. 30.8 +/- 0.6 mmol/kg dry wt) but increased significantly at exhaustion to 33.8 +/- 1.0 mmol/kg dry wt in the trained state and to 33.9 +/- 0.9 mmol/kg dry wt in the detrained state. The elevated muscle [Mg2+] at exhaustion could contribute to fatigue in prolonged exercise through an inhibition of Ca2+ release from sarcoplasmic reticulum. It is concluded that the endurance capacity can vary considerably during detraining without changes in VO2max. Altered substrate utilization or changes in electrolyte regulation may account for the reduced endurance capacity.
Ugeskrift for laeger
The attitude towards the treatment of acute myocardial infarction is currently changing. As an ex... more The attitude towards the treatment of acute myocardial infarction is currently changing. As an example of one of the new methods, a successful case of acute percutaneous transluminal coronary angioplasty is presented. Which of the new therapeutic regimens provides the best prognosis is unknown, but considering the resources and the hitherto published studies, intravenous thrombolysis seems in general to be the most advantageous, while the others ought only to be used on special indications.
Ugeskrift for laeger, Jan 7, 2008
A 66 year-old man presented drug-refractory, repetitive ventricular tachyarrhythmias after acute ... more A 66 year-old man presented drug-refractory, repetitive ventricular tachyarrhythmias after acute myocardial infarction. Despite successful revascularization, the patient suffered recurrent episodes of rapid ventricular tachycardia which could only be managed by radiofrequency ablation.
Upsala Journal of Medical Sciences, 1993
In order to investigate the relevance of the currently used lower reference limit for S-Potassium... more In order to investigate the relevance of the currently used lower reference limit for S-Potassium in Danish hospital laboratories, analytical bias in the measurement of S-Potassium was compared with the lower reference limit in each of 52 Danish hospital ...
Medicine& Science in Sports & Exercise, 1998
The Journal of Clinical Endocrinology & Metabolism, 2001
A reduced functional capacity of the sodium (Na), potassium (K) pump might reduce energy expendit... more A reduced functional capacity of the sodium (Na), potassium (K) pump might reduce energy expenditure, inducing obesity and type 2 diabetes. Consequently, the Na and K content and [ 3 H]ouabain binding capacity of skeletal muscle were measured in 10 monozygotic twin pairs discordant for type 2 diabetes and in 10 obese controls. Muscle [ 3 H]ouabain binding capacity was reduced by approximately 20% in type 2 diabetes. Removing the genetic component by looking at differences within twin pairs, the difference in waist/hip ratio was associated with the difference in [ 3 H]ouabain binding (r ϭ Ϫ0.85; P Ͻ 0.002). Except for the type 2 diabetic twins in the basal state, both basal and insulin-stimulated energy expenditure were associated with the muscle K/Na ratio in the twins. In controls, the 2-h plasma glucose concentration during an oral glucose tolerance test was associated with the change in both muscle and plasma K induced by a euglycemic, hyperinsulinemic clamp. In conclusion, environmental factors related to the waist/hip ratio reduce the muscle [ 3 H]ouabain binding capacity in type 2 diabetes. Without proving causality, the muscle K/Na ratio is associated with energy expenditure in individuals genetically predisposed to the development of type 2 diabetes.
International Journal of Cardiology, 2004
ABSTRACT New non-pharmacological interventions have evolved over the latest decades in order to p... more ABSTRACT New non-pharmacological interventions have evolved over the latest decades in order to prevent atrial fibrillation and/or reduce symptoms. Radiofrequency ablation (RFA) has been performed in Denmark since 2001. In 2011, 2,529 catheter-based RFAs for atrial fibrillation were performed. RFA is now considered a treatment option in patients with drug-refractory atrial fibrillation or young patients who prefer RFA rather than anti-arrhythmic drugs. RFA aims at minimising the impact of "trigger foci" from the pulmonary veins or inhibiting the evolvement of electrical impulses within the atrium.
Analytical Biochemistry, 1998
The aim of the study was to evaluate the use of freeze-dried and dissected small muscle biopsy sp... more The aim of the study was to evaluate the use of freeze-dried and dissected small muscle biopsy specimens ("dry") for the determination of human muscle electrolyte content and ouabain binding capacity, compared with an easier method, without this freezedrying step ("wet"). Freeze-drying and dissection of muscle biopsy specimens reduced the variation in the determination of muscle potassium and magnesium content. The total coefficient of variation was 8.6% in the dry determination of muscle potassium content and 13.5% in the wet determination (P < 0.05). In the determination of muscle magnesium content, the total coefficient of variation was 7.4% in the dry determination and 13.7% when determined wet (P < 0.005). Muscle sodium content had a very large coefficient of variation, independent of the method used. The content of dry solids was too high in biopsies which were incubated in Tris-vanadate buffer (31.9%), compared to biopsies which were not incubated in Tris-vanadate buffer (24.9%, P < 0.001). Hereby, the measured ouabain binding capacity became too high when measured wet. In conclusion, muscle electrolyte content and ouabain binding capacity should be determined after drying and microdissection of the biopsies, because this method confers the least variation and the highest accuracy.
Ugeskrift For Laeger, 2013