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Papers by Subed Chandra Dev Sharma

Science Journal of Chemistry, 2021

Cuminum cyminum is a widely used spice and most valuable medicinal plant in South Asian countries... more Cuminum cyminum is a widely used spice and most valuable medicinal plant in South Asian countries to enhance vitality. This herb has been reported to be used as conventional remedy for many years. In this research, C. cyminum cultivated in Bangladesh was focused on exploring the scavenging ability using different solvents with green extraction technique. This study aims to screen the suitable solvent for the effective C. cyminum extraction followed by the phytochemical analysis and identification of its chemical constituents. The solvents, methanol and n-hexane, were used for the extraction using sonication extraction technique to screen the antioxidant activity of C. cyminum. Moreover, the different solvent ratios (0, 20, 40, 60, 80 and 100%) of ethanol and water were used to investigate the best combination of extracting solvents that can produce C. cyminum extracts with the most desirable and potent antioxidant activities. The metabolites were identified using gas chromatography-mass spectrometry. The methanol extract showed maximum yield (5.32±2.86%) and IC 50 (3.02±1.97) value concerning n-hexane (3.02±0.78%, 5.08±0.95). However, the range of different solvent ratios (100% ethanol-0% ethanol) yield was 4.11±0.59-14.47±5.36%. On the other hand, the 60% ethanol exhibited the highest inhibitory potential (1.39±1.42µg/ml), and aqueous extract exhibited the lowest inhibitory potential (5.79±3.43µg/ml). Phytochemical investigation showed that tannin and glycosides were present in both extracts. Flavonoids showed a positive result in methanol extract, while anthraquinones and steroids showed a positive result in n-hexane extract. The identified metabolites using GCMS are D-carvone, 1, 3-benzodioxole, squalene, 2H, 6H-benzo [1, 2-b: 5, 4-b'] dipyran-2, 6-dione, D-limonene and apiol. Cuminum cyminum can reduce oxidative damage by potently inhibiting free radicals and can be utilized as natural compounds to scavenge free radical activity.

Environmental Technology & Innovation, 2021

Abstract In this study, we have immobilized bacterial cells with mediators as bioaugmentation to ... more Abstract In this study, we have immobilized bacterial cells with mediators as bioaugmentation to increase the numbers of bacterial cells in anodic biofilm for better microbial fuel cell (MFC) performance. Four individual bacterial strains (strain E1, strain SCS5, strain B2 and Shewanella putrefaciens) and their mixed culture were first evaluated for electricity production in MFCs. The mixed culture MFC produced higher power density (3.021 Wm−3) than pure strains. The pre-colonized (PC) biofilms together with mediator immobilized mixed culture (MIMC) beads as bioanodes further enhanced the performance of MFCs. Integrated PC/MIMC MFCs containing anthraquinine-2,6-disulfonate (AQDS) and Fe3O4 immobilized mixed culture beads produced 1.50 and 1.47 times power density with respect to mixed culture biofilm, respectively. AQDS immobilization showed better performance compared to Fe3O4 in energy output. The integrated use of PC biofilms and MIMC exhibited lower charge transfer resistance than corresponding individual anode biofilms in MFCs according to electrochemical impedance spectroscopy analysis. The MFC having PC/MIMC as anode showed the ability to store electrons generated by bacteria, but the biocapacitance of anode was altered after integrating MIMC bead particles. Thus, the collective use of PC biofilm and MIMC beads will be one of the potential choices for improving MFC performance.

International Biodeterioration & Biodegradation, 2016

In this study, the decolorization and degradation of methyl red (MR) by suspended and immobilized... more In this study, the decolorization and degradation of methyl red (MR) by suspended and immobilized cells of Aeromonas jandaei strain SCS5 under anaerobic and aerobic conditions have been investigated. The complete decolorization of MR at a concentration of 100 mg L-1 by A. jandaei strain SCS5 was obtained within 6 h for both anaerobic and aerobic suspended cultures, where the decolorization rate was faster in acidic conditions than basic conditions. The decolorization efficiency under 6 h increased with increasing cell mass of inoculation and decreased with increasing initial dye concentrations. Immobilized cells of A. jandaei strain SCS5 could decolorize MR, and the decolorization rate was significantly enhanced by cells immobilized with mediators such as anthraquinone-2,6-disulphonate and magnetic Fe3O4 nanoparticles compared to immobilized cells only. Moreover, the immobilized bacterial beads with mediators retained high decolorization activity up to more than 10 repeating cycles. UVvisible spectra (200-800 nm) and gas chromatography-mass spectrometry analysis demonstrated that MR was degraded by A. jandaei strain SCS5 through reductive cleavage of azo bond. MR degradation products showed less phytotoxicity against Triticum aestivum and Phaseolus mungo compared to untreated MR. This study has demonstrated that A. jandaei strain SCS5 could be a promising microbiological agent for the removal of azo dyes from the environment.

Journal of microbiology, biotechnology and food sciences

The strain Bacillus methylotrophicus-SCS2012 (Bacillus sp. SCS2012) identified by 16S rRNA gene s... more The strain Bacillus methylotrophicus-SCS2012 (Bacillus sp. SCS2012) identified by 16S rRNA gene sequencing was isolated from soil. Ethyl acetate extract of B. methylotrophicus-SCS2012 showed antibacterial activity against both Gram positive and Gram negative bacteria. The crude metabolite extracted from B. methylotrophicus-SCS2012 exhibited strong antibacterial activity against Streptococcus agalactae, Bacillus cereus, Escherichia coli, Shigella sonnei and Shigella dysenteriae with the zone of inhibition range 28.33 to 32.16 mm at 100μg/disc. Minimum inhibitory concentration (MIC) of the extract against Streptococcus agalactae, Bacillus cereus, Escherichia coli, Shigella sonnei and Shigella dysenteriae was found to be 156, 156, 312, 312 and 625µg/ml respectively. The ethyl acetate extract also exhibited cytotoxic effects on brine shrimp lethality bioassay with LC50 value of 10.78 g/ml. Thus the ethyl acetate extract of Bacillus methylotrophicus-SCS2012 has broad spectrum activity w...

Antibacterial activity; Cytotoxic effect; Micrococcus sp SCS1. KEYWORDS ABSTRACT The xylanase pro... more Antibacterial activity; Cytotoxic effect; Micrococcus sp SCS1. KEYWORDS ABSTRACT The xylanase producing bacterial strains were isolated from bamboo gar-den waste. The strains were isolated on xylan agar media and screening was carried out by xylanolysis method. To test the sensitivity of the iso-lates, seven different antibiotics were used. The strains showed sensitiv-ity to Ampicillin, Amoxycillin, Bacitracin, Chloramphenical, Doxycyclin, Erythromycin and Kanamycin when they were tested by disc diffusion method on nutrient agar plate and confirmed by antibiotic spread plate method. Ethyl acetate was used for solvent extraction of antimicrobial principals from the culture filtrate. The ethyl acetate extract of Micrococ-cus sp SCS1 showed promising antibacterial activity against a number of gram positive (Bacillus subtilis and Staphylococcus aureus) and gram negative (Escherichia coli, Shigella dysenteriae, Shigella sonnei, Shi-gella shiga, Salmonella typhi and Klebsiella Pneumonia) ...

Journal of Bio-Science, 2012

Context: To analyze the nutritional and physicochemical parameters for the production of alkali-t... more Context: To analyze the nutritional and physicochemical parameters for the production of alkali-thermostable and cellulase free xylanase from bacteria. Objectives: The aim of this study was to isolation and identification and of alkali-thermostable and cellulase free xylanase producing bacteria from soil as well as optimization of process parameters for xylanase production. Materials and Methods: The bacterium Bacillus sp. was isolated from soil by serial dilution technique on xylan agar medium and identified by morphological and biochemical studies. The production of xylanase was carried out on xylan broth medium and xylanase activity was assayed by dinitrosalicylic acid (DNS) method. The effect of cultural parameters on the production of xylanase was determined by measuring the activity of xylanase. The effect of temperature and pH on the activity of partially purified xylanase as well as substrate specificity of xylanase were examined. Results: The maximum xylanase production (40...

Microbes and environments / JSME, Jan 9, 2016

A facultative anaerobic bacterium, designated as strain SCS5, was isolated from the anodic biofil... more A facultative anaerobic bacterium, designated as strain SCS5, was isolated from the anodic biofilm of a mediator-less microbial fuel cell using acetate as the electron donor and α-FeOOH as the electron acceptor. The isolate was Gram-negative, motile, and shaped as short rods (0.9-1.3 μm in length and 0.4-0.5 μm in width). A phylogenetic analysis of the 16S rRNA, gyrB, and rpoD genes suggested that strain SCS5 belonged to the Aeromonas genus in the Aeromonadaceae family and exhibited the highest 16S rRNA gene sequence similarity (99.45%) with Aeromonas jandaei ATCC 49568. However, phenotypic, cellular fatty acid profile, and DNA G+C content analyses revealed that there were some distinctions between strain SCS5 and the type strain A. jandaei ATCC 49568. The optimum growth temperature, pH, and NaCl (%) for strain SCS5 were 35°C, 7.0, and 0.5% respectively. The DNA G+C content of strain SCS5 was 59.18%. The isolate SCS5 was capable of reducing insoluble iron oxide (α-FeOOH) and transfe...

Science Journal of Chemistry, 2021

Cuminum cyminum is a widely used spice and most valuable medicinal plant in South Asian countries... more Cuminum cyminum is a widely used spice and most valuable medicinal plant in South Asian countries to enhance vitality. This herb has been reported to be used as conventional remedy for many years. In this research, C. cyminum cultivated in Bangladesh was focused on exploring the scavenging ability using different solvents with green extraction technique. This study aims to screen the suitable solvent for the effective C. cyminum extraction followed by the phytochemical analysis and identification of its chemical constituents. The solvents, methanol and n-hexane, were used for the extraction using sonication extraction technique to screen the antioxidant activity of C. cyminum. Moreover, the different solvent ratios (0, 20, 40, 60, 80 and 100%) of ethanol and water were used to investigate the best combination of extracting solvents that can produce C. cyminum extracts with the most desirable and potent antioxidant activities. The metabolites were identified using gas chromatography-mass spectrometry. The methanol extract showed maximum yield (5.32±2.86%) and IC 50 (3.02±1.97) value concerning n-hexane (3.02±0.78%, 5.08±0.95). However, the range of different solvent ratios (100% ethanol-0% ethanol) yield was 4.11±0.59-14.47±5.36%. On the other hand, the 60% ethanol exhibited the highest inhibitory potential (1.39±1.42µg/ml), and aqueous extract exhibited the lowest inhibitory potential (5.79±3.43µg/ml). Phytochemical investigation showed that tannin and glycosides were present in both extracts. Flavonoids showed a positive result in methanol extract, while anthraquinones and steroids showed a positive result in n-hexane extract. The identified metabolites using GCMS are D-carvone, 1, 3-benzodioxole, squalene, 2H, 6H-benzo [1, 2-b: 5, 4-b'] dipyran-2, 6-dione, D-limonene and apiol. Cuminum cyminum can reduce oxidative damage by potently inhibiting free radicals and can be utilized as natural compounds to scavenge free radical activity.

Environmental Technology & Innovation, 2021

Abstract In this study, we have immobilized bacterial cells with mediators as bioaugmentation to ... more Abstract In this study, we have immobilized bacterial cells with mediators as bioaugmentation to increase the numbers of bacterial cells in anodic biofilm for better microbial fuel cell (MFC) performance. Four individual bacterial strains (strain E1, strain SCS5, strain B2 and Shewanella putrefaciens) and their mixed culture were first evaluated for electricity production in MFCs. The mixed culture MFC produced higher power density (3.021 Wm−3) than pure strains. The pre-colonized (PC) biofilms together with mediator immobilized mixed culture (MIMC) beads as bioanodes further enhanced the performance of MFCs. Integrated PC/MIMC MFCs containing anthraquinine-2,6-disulfonate (AQDS) and Fe3O4 immobilized mixed culture beads produced 1.50 and 1.47 times power density with respect to mixed culture biofilm, respectively. AQDS immobilization showed better performance compared to Fe3O4 in energy output. The integrated use of PC biofilms and MIMC exhibited lower charge transfer resistance than corresponding individual anode biofilms in MFCs according to electrochemical impedance spectroscopy analysis. The MFC having PC/MIMC as anode showed the ability to store electrons generated by bacteria, but the biocapacitance of anode was altered after integrating MIMC bead particles. Thus, the collective use of PC biofilm and MIMC beads will be one of the potential choices for improving MFC performance.

International Biodeterioration & Biodegradation, 2016

In this study, the decolorization and degradation of methyl red (MR) by suspended and immobilized... more In this study, the decolorization and degradation of methyl red (MR) by suspended and immobilized cells of Aeromonas jandaei strain SCS5 under anaerobic and aerobic conditions have been investigated. The complete decolorization of MR at a concentration of 100 mg L-1 by A. jandaei strain SCS5 was obtained within 6 h for both anaerobic and aerobic suspended cultures, where the decolorization rate was faster in acidic conditions than basic conditions. The decolorization efficiency under 6 h increased with increasing cell mass of inoculation and decreased with increasing initial dye concentrations. Immobilized cells of A. jandaei strain SCS5 could decolorize MR, and the decolorization rate was significantly enhanced by cells immobilized with mediators such as anthraquinone-2,6-disulphonate and magnetic Fe3O4 nanoparticles compared to immobilized cells only. Moreover, the immobilized bacterial beads with mediators retained high decolorization activity up to more than 10 repeating cycles. UVvisible spectra (200-800 nm) and gas chromatography-mass spectrometry analysis demonstrated that MR was degraded by A. jandaei strain SCS5 through reductive cleavage of azo bond. MR degradation products showed less phytotoxicity against Triticum aestivum and Phaseolus mungo compared to untreated MR. This study has demonstrated that A. jandaei strain SCS5 could be a promising microbiological agent for the removal of azo dyes from the environment.

Journal of microbiology, biotechnology and food sciences

The strain Bacillus methylotrophicus-SCS2012 (Bacillus sp. SCS2012) identified by 16S rRNA gene s... more The strain Bacillus methylotrophicus-SCS2012 (Bacillus sp. SCS2012) identified by 16S rRNA gene sequencing was isolated from soil. Ethyl acetate extract of B. methylotrophicus-SCS2012 showed antibacterial activity against both Gram positive and Gram negative bacteria. The crude metabolite extracted from B. methylotrophicus-SCS2012 exhibited strong antibacterial activity against Streptococcus agalactae, Bacillus cereus, Escherichia coli, Shigella sonnei and Shigella dysenteriae with the zone of inhibition range 28.33 to 32.16 mm at 100μg/disc. Minimum inhibitory concentration (MIC) of the extract against Streptococcus agalactae, Bacillus cereus, Escherichia coli, Shigella sonnei and Shigella dysenteriae was found to be 156, 156, 312, 312 and 625µg/ml respectively. The ethyl acetate extract also exhibited cytotoxic effects on brine shrimp lethality bioassay with LC50 value of 10.78 g/ml. Thus the ethyl acetate extract of Bacillus methylotrophicus-SCS2012 has broad spectrum activity w...

Antibacterial activity; Cytotoxic effect; Micrococcus sp SCS1. KEYWORDS ABSTRACT The xylanase pro... more Antibacterial activity; Cytotoxic effect; Micrococcus sp SCS1. KEYWORDS ABSTRACT The xylanase producing bacterial strains were isolated from bamboo gar-den waste. The strains were isolated on xylan agar media and screening was carried out by xylanolysis method. To test the sensitivity of the iso-lates, seven different antibiotics were used. The strains showed sensitiv-ity to Ampicillin, Amoxycillin, Bacitracin, Chloramphenical, Doxycyclin, Erythromycin and Kanamycin when they were tested by disc diffusion method on nutrient agar plate and confirmed by antibiotic spread plate method. Ethyl acetate was used for solvent extraction of antimicrobial principals from the culture filtrate. The ethyl acetate extract of Micrococ-cus sp SCS1 showed promising antibacterial activity against a number of gram positive (Bacillus subtilis and Staphylococcus aureus) and gram negative (Escherichia coli, Shigella dysenteriae, Shigella sonnei, Shi-gella shiga, Salmonella typhi and Klebsiella Pneumonia) ...

Journal of Bio-Science, 2012

Context: To analyze the nutritional and physicochemical parameters for the production of alkali-t... more Context: To analyze the nutritional and physicochemical parameters for the production of alkali-thermostable and cellulase free xylanase from bacteria. Objectives: The aim of this study was to isolation and identification and of alkali-thermostable and cellulase free xylanase producing bacteria from soil as well as optimization of process parameters for xylanase production. Materials and Methods: The bacterium Bacillus sp. was isolated from soil by serial dilution technique on xylan agar medium and identified by morphological and biochemical studies. The production of xylanase was carried out on xylan broth medium and xylanase activity was assayed by dinitrosalicylic acid (DNS) method. The effect of cultural parameters on the production of xylanase was determined by measuring the activity of xylanase. The effect of temperature and pH on the activity of partially purified xylanase as well as substrate specificity of xylanase were examined. Results: The maximum xylanase production (40...

Microbes and environments / JSME, Jan 9, 2016

A facultative anaerobic bacterium, designated as strain SCS5, was isolated from the anodic biofil... more A facultative anaerobic bacterium, designated as strain SCS5, was isolated from the anodic biofilm of a mediator-less microbial fuel cell using acetate as the electron donor and α-FeOOH as the electron acceptor. The isolate was Gram-negative, motile, and shaped as short rods (0.9-1.3 μm in length and 0.4-0.5 μm in width). A phylogenetic analysis of the 16S rRNA, gyrB, and rpoD genes suggested that strain SCS5 belonged to the Aeromonas genus in the Aeromonadaceae family and exhibited the highest 16S rRNA gene sequence similarity (99.45%) with Aeromonas jandaei ATCC 49568. However, phenotypic, cellular fatty acid profile, and DNA G+C content analyses revealed that there were some distinctions between strain SCS5 and the type strain A. jandaei ATCC 49568. The optimum growth temperature, pH, and NaCl (%) for strain SCS5 were 35°C, 7.0, and 0.5% respectively. The DNA G+C content of strain SCS5 was 59.18%. The isolate SCS5 was capable of reducing insoluble iron oxide (α-FeOOH) and transfe...