Subhajit Mondal - Academia.edu (original) (raw)
Papers by Subhajit Mondal
American Journal of Plant Sciences
Proceedings of the 31st ACM International Conference on Information & Knowledge Management
Lumpy skin disease (LSD), a notifiable disease listed by the World Organization for Animal Health... more Lumpy skin disease (LSD), a notifiable disease listed by the World Organization for Animal Health and a fast fast-moving transboundary viral disease infecting cattle and buffaloes, was reported in India in 2019 and has since rapidly spread across the country. This study reports the first complete genome sequence and analysis of a pathogenic LSD virus (LSDV) from India (LSDV/208/PVNRTVU/2020) obtained by direct sequencing of a suspected clinical sample using Illumina and Nanopore sequencing technologies. The complete genome sequence of LSDV/208/PVNRTVU/2020 is 150445 bp long, codes for 156 putative genes and carries identical 2254 bp inverted terminal repeats at either ends. The unique features reported in the LSDV isolates from the recent outbreaks in Asia, namely, the insertions of 12 nucleotides in the viral G-protein coupled receptor (GPCR) and 27 nucleotides leading to duplication of 9 aminoacids in the extracellular enveloped virus-specific (EEV) genes were also conserved in LS...
International Journal of Structural Stability and Dynamics
Sandwich composite structures may have a significant variation in their dynamic behavior due to t... more Sandwich composite structures may have a significant variation in their dynamic behavior due to the uncertain preconditions of the manufacturing processes, material properties, geometry, etc. Therefore, quantifying and modeling such variability in the dynamic behavior of sandwich composite structures is a necessity. In this study, free vibration analysis is carried out for a glass-fiber reinforced plastic (GFRP) sandwich composite plate. The natural frequencies are obtained by taking into account various uncertainties involved in preparing the sandwich composite plate. The Kolmogorov–Smirnov (KS) and Anderson–Darling (AD) goodness-of-fit (GOF) tests are performed for the quantification of the uncertainty of the natural frequencies. The appropriate probabilistic distribution describing the variability of the vibrational response is recommended. The knowledge of the uncertainty of dynamic responses might help to control the quality of the manufacturing process, as well as for the reli...
Angewandte Chemie
Ladderane phospholipids, with their unusual ladder-like arrangement of concatenated cyclobutane r... more Ladderane phospholipids, with their unusual ladder-like arrangement of concatenated cyclobutane rings, represent an architecturally unique class of natural products. However, despite their fascinating structure and other necessary impetus, only a few synthetic studies of these molecules have been reported so far. We have now devised a concise total synthesis of [3]-ladderanol, a component of ladderane phospholipids, using an organocatalytic enantioselective desymmetrizing formal C(sp2)-H alkylation. Our synthetic strategy rests on the late-stage introduction of chirality, thus allowing facile access to both enantiomers of [3]-ladderanol as well as an analogue. This is the first time a desymmetrization strategy is applied to the synthesis of [3]-ladderanol. The scope of this desymmetrizing C(sp2)-H alkylation of meso-cyclobutane-fused cyclohexenediones is also presented.
Journal of Engineering and Applied Science, 2022
Recently, the use of sandwich composites in different fields of engineering such as aerospace, ma... more Recently, the use of sandwich composites in different fields of engineering such as aerospace, marine, automobile, pipelines, bridge structure, industrial work, has attracted significant attention. Sensitivity analysis of structures made of sandwich composites is necessary to design them properly and maintain their longevity. The present study analyzes stiffened sandwich composite bridge deck panels and focuses on its sensitivity analysis. The lack of control in the manufacturing of the sandwich composites may lead to non-uniform material properties, and thus variation in its behavior. The variation in the dynamic responses obtained through the free vibration analysis of the bridge deck panel models of stiffened composites due to is studied. The free vibration analysis is implemented using a finite element method. The analysis is carried out with the stiffeners located in different positions and alignments. The glass fiber-reinforced plastic (GFRP) and polyvinyl semi-rigid foam are ...
An investigation has been carried out for identification of both in-plane and out-of-plane elasti... more An investigation has been carried out for identification of both in-plane and out-of-plane elastic parameters of orthotropic composite structures from dynamic responses. A gradient based finite element model updating approach is used to implement the same. The methodology is based on the comparison of modal parameters of composite plates and beams, as obtained from finite element analysis as well as from modal testing. A three-step identification methodology is proposed in which first the in-plane elastic moduli are estimated from observed modes of plate specimens, followed by estimation of out-of-plane shear moduli from separate modal testing of two beam specimens which are oriented along the longer and shorted axes of the rectangular plate. Experimental modal testing data are numerically simulated for the present investigation. Since the in-plane and the out-of-plane elastic parameters have varied order of sensitivities associated with different sets of modes, it is virtually impossible to update all the in-plane and out-of-plane parameters together from a single orthotropic plate type of structures and use of beam specimens oriented in appropriate directions are mandatory. The present method recognizes this difficulty of parameter estimation algorithms to determine material properties of orthotropic plates and uses the multi-step estimation process successfully. The results show rapid conver-gences from varied ranges of initial guesses of parameters and the methodology is found to be robust.
Renewable energy has too much attention over past few years specially solar energy. Photovoltaic ... more Renewable energy has too much attention over past few years specially solar energy. Photovoltaic is a technique in which solar energy is converted into electrical energy (DC). As we know conventional energy is limited so we are trying to improve the uses of renewable energy like solar energy, hydel energy, and tidal energy. A single cell of photovoltaic has still very low output so it is necessary to improve the performance and reduce the cost. The model of photovoltaic presented in this paper can be used to visualize its output characteristics which are I-V characteristics and P-V characteristics under different irradiation level and temperature. In this paper MPPT-Maximum power point tracking is a method in which changing the ratio between current and voltage delivered to get power by tracking one maximum power point from array input. Here the system developed by combining (PV) photovoltaic module and DC-DC boost converter. There is a detail discussion of DC-DC boost converter and...
![Research paper thumbnail of Enantioselective Synthesis of [3]-Ladderanol through a Late-Stage Organocatalytic Desymmetrization](https://attachments.academia-assets.com/102989367/thumbnails/1.jpg)
](Ladderane phospholipids, with their unusual ladder-like arrangement of fused cyclobutane rings, r... more Ladderane phospholipids, with their unusual ladder-like arrangement of fused cyclobutane rings, represent an architecturally unique class of natural products. However, despite their fascinating structure and other necessary impetus, only a few synthetic studies of these molecules have been reported so far. We have now devised a concise synthesis of [3]-ladderanol, a component of ladderane phospholipids, using an organocatalytic enantioselective desymmetrizing formal C(sp2)‒H alkylation. Our synthetic strategy rests on a late-stage introduction of chirality, thus allowing facile access to both the enantiomers of [3] ladderanol as well as its analog. This is the first time a desymmetrization strategy is applied to the synthesis of [3]-ladderanol. The scope of this desymmetrizing C(sp2)‒H alkylation of meso cyclobutane fused cyclohexenediones is also presented.
Journal of Vibration and Control, 2021
A complex eigenvector is a result of nonproportional damping present in a structural system. Howe... more A complex eigenvector is a result of nonproportional damping present in a structural system. However, it is difficult to identify the accurate damping matrix considering the modal sparsity and coordinate sparsity. A nonproportional viscous damping parameter identification is formulated as an unconstrained optimization problem in the present study. The damping coefficient of each element is considered as the design variable for the optimization problem. The objective function is defined using the incomplete complex eigenvectors, which are generated because of the presence of external damping devices in the structure. This objective function is then minimized using standard particle swarm optimization to identify the damping coefficient of the damping matrix. The accuracy and efficiency of the particle swarm optimization are investigated by solving a few numerical problems with simulated measured data. The proposed method works well with the incomplete measured modal data. The current...
2019 18th European Control Conference (ECC), 2019
In this paper we study a class of linear quadratic Stackelberg difference games with constraints.... more In this paper we study a class of linear quadratic Stackelberg difference games with constraints. We assume that the leader has two sets of strategies. He announces only one set of strategies and the other set strategies are not announced but are constrained. We formulate the necessary conditions for the existence of Stackelberg equilibrium for this class of games. In particular, we show under a few assumptions, the necessary conditions can be recasted as a single large scale complementarity problem. We also provide sufficient conditions under which the solution of this complementarity problem is a Stackelbreg equilibrium.
International Journal of Acoustics and Vibration, 2018
A combined numerical-experimental technique has been implemented using the finite element model u... more A combined numerical-experimental technique has been implemented using the finite element model updating technique to estimate a set of proportional viscous damping parameters for determining the global responses of fibre reinforced plastic (FRP) plates over a chosen frequency range of interest. The experimentally determined frequencies and mode shapes are used to update the homogenised anisotropic in-plane material constants, before estimating the damping parameters from the directly observed frequency response functions (FRFs). Gradient based inverse sensitivity method has been implemented for the parameter estimation. Existing FRP structures may degrade considerably due to environmental effects over the long period of existence—changing the material and damping properties significantly compared to their initial values after fabrication—thus requiring updating. The estimated viscous damping parameters using the current technique reproduces higher values of modal damping factors fo...
Molecular Biology Reports, 2021
BACKGROUND The physiological significance of a large family of heat-shock proteins (HSPs), compri... more BACKGROUND The physiological significance of a large family of heat-shock proteins (HSPs), comprised of the cytosolic HSP90A and the endoplasmic reticulum component of HSPB, is evident in prokaryotes and eukaryotes. The HSP90A is believed to play critical roles in diverse physiological functions of cell viability and chromosomal stability including stress management. Heightened abundance of hsp90β transcript was documented in Channa striatus, a freshwater fish, which is capable of surviving within an extremely hypoxic environment. METHODS AND RESULTS To better understand the mechanism of hsp90β gene expression, we investigated its genomic organization. Eleven exons were identified, including a long upstream intron with a remarkable similarity with human, but not with chicken counterpart. Dual-luciferase assays identified promoter activity in a 1366 bp 5'-flanking segment beyond the transcription initiation site. Examination detected a minimal promoter of 754 bp containing a TATA-box, CAAT-enhancer in addition to providing clues regarding other enhancer and repressor elements. The driving capability of this minimal promoter was further validated by its binding ability with TATA-box binding protein and the generation of GFP expressing transgenic zebrafish (F2). Further, deletion of an inverted HIF (hypoxia inducible factor) motif RCGTG (upstream of the TATA-box) dramatically reduced luciferase expression in a hypoxic environment (CoCl2 treated cultivable cells) and was identified as a cis-acting HIF responsive element, necessary for the hypoxia-induced expression. CONCLUSIONS The results obtained herein provide an insight regarding how hsp90β gene expression is controlled by HIF responsive element in teleost both during hypoxia stress management and normal physiological functions, and suggested that the hsp90β gene promoter could be used as a potential candidate for generating ornamental and food-fish transgenics.
Indian Journal of Genetics and Plant Breeding (The), 2019
Recent advances in high throughout DNA sequencing technologies have revolutionized for better und... more Recent advances in high throughout DNA sequencing technologies have revolutionized for better understanding of structure-functional relationships of genes in identifying trait-associated transcriptomes and their regulated gene expressions. Subsequent breakthroughs in gene editing technologies such as zinc finger nucleases, transcriptional activator-like effect or nucleases (TALEN) and CRISPR (clustered regularly interspaced short palindromic repeats) determined chromosomal loci so as to understand gene functions in vivo. Such editing technologies are now being implemented in many laboratories due to an affordable cost and easiness of techniques. Targeted gene delivery and disruptions are now not only restricted to standard cell lines or stem cells, but also primary cell lines and nonmodel agriculturally important species. Progress and implications of gene integration and disruptions in food fishes like salmon, carps, etc. will be highlighted. The positive impacts on myostatin gene (...
Gene Reports, 2019
Abstract The promyelocytic leukemia zinc finger (Plzf) gene, being a transcriptional repressor, p... more Abstract The promyelocytic leukemia zinc finger (Plzf) gene, being a transcriptional repressor, plays a crucial role in self-renewal and maintenance of spermatogonial stem cells (SSCs) across mammalian and non-mammalian species. It is now being considered as a candidate marker of proliferating SSC in teleost. The knowledge on its mechanism of expression is limited. In this study, we intended to clone and characterize Plzf gene promoter of Clarias batrachus, a commercially valuable farmed catfish (also known as magur). A comparative cDNA sequence analysis between Labeo rohita carp and C. batrachus catfish confirmed about perfect homology. We have isolated, sequenced and characterized the regulatory elements of Plzf by genome walking. About 3.6 kb sequence of 5′ upstream region relative to ATG start codon was sequenced. Alignment of genome walking and cDNA sequence data identified transcriptional start site (TSS). The 5′ UTR comprises of non-coding exons (EI and EII) containing a long stretch of intron. Computational analysis revealed several important putative elements like vertebrate TATA binding protein factor (VTBP), ecotropic viral integration site (Evi-1), myoblast determining factors (MyoD), myocyte-specific enhancer binding factor (MEF2), general transcription factor IID initiator (TFIID), etc.; positioned in the upstream sequence above transcriptional start site; indicating as potential promoter/regulatory elements. Dual luciferase reporter assay was performed for various deleted constructs designed to measure their promoter activity in vivo. Its full promoter activity was dependent on upstream region containing a putative Evi-1-like element as enhancer. We also identified the region possibly acting as a transcriptional repressor (Gfi-1). This is the first report of identifying Plzf gene being expressed in spermatogonial cells of C. batrachus. Even though the Plzf cDNA sequence is conserved across species, its regulatory elements are not conserved among human, carps and catfishes.
Cancer and Clinical Oncology, 2018
Aims: To identify and estimate salivary VEGF of breast cancer patients using non invasive method.... more Aims: To identify and estimate salivary VEGF of breast cancer patients using non invasive method. Methods: ELISA, Zymography, Immunoblot. Results: We observed that saliva of breast cancer patients express appreciably high VEGF compare to non breast cancer saliva samples. Conclusion: The findings indicate salivary VEGF as a potential breast cancer marker using non invasive method.
Journal of Vibration and Control, 2016
A methodology has been proposed to estimate non-proportional viscous damping matrix of beams from... more A methodology has been proposed to estimate non-proportional viscous damping matrix of beams from measured complex eigendata using finite element model updating technique. Representation of damping through a proportional damping matrix ignoring the complexity of eigenvectors may not be appropriate when external damping devices are employed. The current literature of determination of non-proportional damping matrix demands measurement of a large number of complex modes which is extremely difficult in practice. A gradient based finite element model updating algorithm implementing inverse eigensensitivity method has been presented through a series of numerically simulated cantilever beams. The method can accurately predict the non-proportional damping matrix even if the measured eigenvectors are polluted with random noise. The novelty of the current method is that it can sustain a high level of modal and coordinate sparsity in measurement. The method assumes prior determination or upda...
International Journal of Mechanical Sciences, 2017
American Journal of Plant Sciences, 2017
American Journal of Plant Sciences
Proceedings of the 31st ACM International Conference on Information & Knowledge Management
Lumpy skin disease (LSD), a notifiable disease listed by the World Organization for Animal Health... more Lumpy skin disease (LSD), a notifiable disease listed by the World Organization for Animal Health and a fast fast-moving transboundary viral disease infecting cattle and buffaloes, was reported in India in 2019 and has since rapidly spread across the country. This study reports the first complete genome sequence and analysis of a pathogenic LSD virus (LSDV) from India (LSDV/208/PVNRTVU/2020) obtained by direct sequencing of a suspected clinical sample using Illumina and Nanopore sequencing technologies. The complete genome sequence of LSDV/208/PVNRTVU/2020 is 150445 bp long, codes for 156 putative genes and carries identical 2254 bp inverted terminal repeats at either ends. The unique features reported in the LSDV isolates from the recent outbreaks in Asia, namely, the insertions of 12 nucleotides in the viral G-protein coupled receptor (GPCR) and 27 nucleotides leading to duplication of 9 aminoacids in the extracellular enveloped virus-specific (EEV) genes were also conserved in LS...
International Journal of Structural Stability and Dynamics
Sandwich composite structures may have a significant variation in their dynamic behavior due to t... more Sandwich composite structures may have a significant variation in their dynamic behavior due to the uncertain preconditions of the manufacturing processes, material properties, geometry, etc. Therefore, quantifying and modeling such variability in the dynamic behavior of sandwich composite structures is a necessity. In this study, free vibration analysis is carried out for a glass-fiber reinforced plastic (GFRP) sandwich composite plate. The natural frequencies are obtained by taking into account various uncertainties involved in preparing the sandwich composite plate. The Kolmogorov–Smirnov (KS) and Anderson–Darling (AD) goodness-of-fit (GOF) tests are performed for the quantification of the uncertainty of the natural frequencies. The appropriate probabilistic distribution describing the variability of the vibrational response is recommended. The knowledge of the uncertainty of dynamic responses might help to control the quality of the manufacturing process, as well as for the reli...
Angewandte Chemie
Ladderane phospholipids, with their unusual ladder-like arrangement of concatenated cyclobutane r... more Ladderane phospholipids, with their unusual ladder-like arrangement of concatenated cyclobutane rings, represent an architecturally unique class of natural products. However, despite their fascinating structure and other necessary impetus, only a few synthetic studies of these molecules have been reported so far. We have now devised a concise total synthesis of [3]-ladderanol, a component of ladderane phospholipids, using an organocatalytic enantioselective desymmetrizing formal C(sp2)-H alkylation. Our synthetic strategy rests on the late-stage introduction of chirality, thus allowing facile access to both enantiomers of [3]-ladderanol as well as an analogue. This is the first time a desymmetrization strategy is applied to the synthesis of [3]-ladderanol. The scope of this desymmetrizing C(sp2)-H alkylation of meso-cyclobutane-fused cyclohexenediones is also presented.
Journal of Engineering and Applied Science, 2022
Recently, the use of sandwich composites in different fields of engineering such as aerospace, ma... more Recently, the use of sandwich composites in different fields of engineering such as aerospace, marine, automobile, pipelines, bridge structure, industrial work, has attracted significant attention. Sensitivity analysis of structures made of sandwich composites is necessary to design them properly and maintain their longevity. The present study analyzes stiffened sandwich composite bridge deck panels and focuses on its sensitivity analysis. The lack of control in the manufacturing of the sandwich composites may lead to non-uniform material properties, and thus variation in its behavior. The variation in the dynamic responses obtained through the free vibration analysis of the bridge deck panel models of stiffened composites due to is studied. The free vibration analysis is implemented using a finite element method. The analysis is carried out with the stiffeners located in different positions and alignments. The glass fiber-reinforced plastic (GFRP) and polyvinyl semi-rigid foam are ...
An investigation has been carried out for identification of both in-plane and out-of-plane elasti... more An investigation has been carried out for identification of both in-plane and out-of-plane elastic parameters of orthotropic composite structures from dynamic responses. A gradient based finite element model updating approach is used to implement the same. The methodology is based on the comparison of modal parameters of composite plates and beams, as obtained from finite element analysis as well as from modal testing. A three-step identification methodology is proposed in which first the in-plane elastic moduli are estimated from observed modes of plate specimens, followed by estimation of out-of-plane shear moduli from separate modal testing of two beam specimens which are oriented along the longer and shorted axes of the rectangular plate. Experimental modal testing data are numerically simulated for the present investigation. Since the in-plane and the out-of-plane elastic parameters have varied order of sensitivities associated with different sets of modes, it is virtually impossible to update all the in-plane and out-of-plane parameters together from a single orthotropic plate type of structures and use of beam specimens oriented in appropriate directions are mandatory. The present method recognizes this difficulty of parameter estimation algorithms to determine material properties of orthotropic plates and uses the multi-step estimation process successfully. The results show rapid conver-gences from varied ranges of initial guesses of parameters and the methodology is found to be robust.
Renewable energy has too much attention over past few years specially solar energy. Photovoltaic ... more Renewable energy has too much attention over past few years specially solar energy. Photovoltaic is a technique in which solar energy is converted into electrical energy (DC). As we know conventional energy is limited so we are trying to improve the uses of renewable energy like solar energy, hydel energy, and tidal energy. A single cell of photovoltaic has still very low output so it is necessary to improve the performance and reduce the cost. The model of photovoltaic presented in this paper can be used to visualize its output characteristics which are I-V characteristics and P-V characteristics under different irradiation level and temperature. In this paper MPPT-Maximum power point tracking is a method in which changing the ratio between current and voltage delivered to get power by tracking one maximum power point from array input. Here the system developed by combining (PV) photovoltaic module and DC-DC boost converter. There is a detail discussion of DC-DC boost converter and...
Ladderane phospholipids, with their unusual ladder-like arrangement of fused cyclobutane rings, r... more Ladderane phospholipids, with their unusual ladder-like arrangement of fused cyclobutane rings, represent an architecturally unique class of natural products. However, despite their fascinating structure and other necessary impetus, only a few synthetic studies of these molecules have been reported so far. We have now devised a concise synthesis of [3]-ladderanol, a component of ladderane phospholipids, using an organocatalytic enantioselective desymmetrizing formal C(sp2)‒H alkylation. Our synthetic strategy rests on a late-stage introduction of chirality, thus allowing facile access to both the enantiomers of [3] ladderanol as well as its analog. This is the first time a desymmetrization strategy is applied to the synthesis of [3]-ladderanol. The scope of this desymmetrizing C(sp2)‒H alkylation of meso cyclobutane fused cyclohexenediones is also presented.
Journal of Vibration and Control, 2021
A complex eigenvector is a result of nonproportional damping present in a structural system. Howe... more A complex eigenvector is a result of nonproportional damping present in a structural system. However, it is difficult to identify the accurate damping matrix considering the modal sparsity and coordinate sparsity. A nonproportional viscous damping parameter identification is formulated as an unconstrained optimization problem in the present study. The damping coefficient of each element is considered as the design variable for the optimization problem. The objective function is defined using the incomplete complex eigenvectors, which are generated because of the presence of external damping devices in the structure. This objective function is then minimized using standard particle swarm optimization to identify the damping coefficient of the damping matrix. The accuracy and efficiency of the particle swarm optimization are investigated by solving a few numerical problems with simulated measured data. The proposed method works well with the incomplete measured modal data. The current...
2019 18th European Control Conference (ECC), 2019
In this paper we study a class of linear quadratic Stackelberg difference games with constraints.... more In this paper we study a class of linear quadratic Stackelberg difference games with constraints. We assume that the leader has two sets of strategies. He announces only one set of strategies and the other set strategies are not announced but are constrained. We formulate the necessary conditions for the existence of Stackelberg equilibrium for this class of games. In particular, we show under a few assumptions, the necessary conditions can be recasted as a single large scale complementarity problem. We also provide sufficient conditions under which the solution of this complementarity problem is a Stackelbreg equilibrium.
International Journal of Acoustics and Vibration, 2018
A combined numerical-experimental technique has been implemented using the finite element model u... more A combined numerical-experimental technique has been implemented using the finite element model updating technique to estimate a set of proportional viscous damping parameters for determining the global responses of fibre reinforced plastic (FRP) plates over a chosen frequency range of interest. The experimentally determined frequencies and mode shapes are used to update the homogenised anisotropic in-plane material constants, before estimating the damping parameters from the directly observed frequency response functions (FRFs). Gradient based inverse sensitivity method has been implemented for the parameter estimation. Existing FRP structures may degrade considerably due to environmental effects over the long period of existence—changing the material and damping properties significantly compared to their initial values after fabrication—thus requiring updating. The estimated viscous damping parameters using the current technique reproduces higher values of modal damping factors fo...
Molecular Biology Reports, 2021
BACKGROUND The physiological significance of a large family of heat-shock proteins (HSPs), compri... more BACKGROUND The physiological significance of a large family of heat-shock proteins (HSPs), comprised of the cytosolic HSP90A and the endoplasmic reticulum component of HSPB, is evident in prokaryotes and eukaryotes. The HSP90A is believed to play critical roles in diverse physiological functions of cell viability and chromosomal stability including stress management. Heightened abundance of hsp90β transcript was documented in Channa striatus, a freshwater fish, which is capable of surviving within an extremely hypoxic environment. METHODS AND RESULTS To better understand the mechanism of hsp90β gene expression, we investigated its genomic organization. Eleven exons were identified, including a long upstream intron with a remarkable similarity with human, but not with chicken counterpart. Dual-luciferase assays identified promoter activity in a 1366 bp 5'-flanking segment beyond the transcription initiation site. Examination detected a minimal promoter of 754 bp containing a TATA-box, CAAT-enhancer in addition to providing clues regarding other enhancer and repressor elements. The driving capability of this minimal promoter was further validated by its binding ability with TATA-box binding protein and the generation of GFP expressing transgenic zebrafish (F2). Further, deletion of an inverted HIF (hypoxia inducible factor) motif RCGTG (upstream of the TATA-box) dramatically reduced luciferase expression in a hypoxic environment (CoCl2 treated cultivable cells) and was identified as a cis-acting HIF responsive element, necessary for the hypoxia-induced expression. CONCLUSIONS The results obtained herein provide an insight regarding how hsp90β gene expression is controlled by HIF responsive element in teleost both during hypoxia stress management and normal physiological functions, and suggested that the hsp90β gene promoter could be used as a potential candidate for generating ornamental and food-fish transgenics.
Indian Journal of Genetics and Plant Breeding (The), 2019
Recent advances in high throughout DNA sequencing technologies have revolutionized for better und... more Recent advances in high throughout DNA sequencing technologies have revolutionized for better understanding of structure-functional relationships of genes in identifying trait-associated transcriptomes and their regulated gene expressions. Subsequent breakthroughs in gene editing technologies such as zinc finger nucleases, transcriptional activator-like effect or nucleases (TALEN) and CRISPR (clustered regularly interspaced short palindromic repeats) determined chromosomal loci so as to understand gene functions in vivo. Such editing technologies are now being implemented in many laboratories due to an affordable cost and easiness of techniques. Targeted gene delivery and disruptions are now not only restricted to standard cell lines or stem cells, but also primary cell lines and nonmodel agriculturally important species. Progress and implications of gene integration and disruptions in food fishes like salmon, carps, etc. will be highlighted. The positive impacts on myostatin gene (...
Gene Reports, 2019
Abstract The promyelocytic leukemia zinc finger (Plzf) gene, being a transcriptional repressor, p... more Abstract The promyelocytic leukemia zinc finger (Plzf) gene, being a transcriptional repressor, plays a crucial role in self-renewal and maintenance of spermatogonial stem cells (SSCs) across mammalian and non-mammalian species. It is now being considered as a candidate marker of proliferating SSC in teleost. The knowledge on its mechanism of expression is limited. In this study, we intended to clone and characterize Plzf gene promoter of Clarias batrachus, a commercially valuable farmed catfish (also known as magur). A comparative cDNA sequence analysis between Labeo rohita carp and C. batrachus catfish confirmed about perfect homology. We have isolated, sequenced and characterized the regulatory elements of Plzf by genome walking. About 3.6 kb sequence of 5′ upstream region relative to ATG start codon was sequenced. Alignment of genome walking and cDNA sequence data identified transcriptional start site (TSS). The 5′ UTR comprises of non-coding exons (EI and EII) containing a long stretch of intron. Computational analysis revealed several important putative elements like vertebrate TATA binding protein factor (VTBP), ecotropic viral integration site (Evi-1), myoblast determining factors (MyoD), myocyte-specific enhancer binding factor (MEF2), general transcription factor IID initiator (TFIID), etc.; positioned in the upstream sequence above transcriptional start site; indicating as potential promoter/regulatory elements. Dual luciferase reporter assay was performed for various deleted constructs designed to measure their promoter activity in vivo. Its full promoter activity was dependent on upstream region containing a putative Evi-1-like element as enhancer. We also identified the region possibly acting as a transcriptional repressor (Gfi-1). This is the first report of identifying Plzf gene being expressed in spermatogonial cells of C. batrachus. Even though the Plzf cDNA sequence is conserved across species, its regulatory elements are not conserved among human, carps and catfishes.
Cancer and Clinical Oncology, 2018
Aims: To identify and estimate salivary VEGF of breast cancer patients using non invasive method.... more Aims: To identify and estimate salivary VEGF of breast cancer patients using non invasive method. Methods: ELISA, Zymography, Immunoblot. Results: We observed that saliva of breast cancer patients express appreciably high VEGF compare to non breast cancer saliva samples. Conclusion: The findings indicate salivary VEGF as a potential breast cancer marker using non invasive method.
Journal of Vibration and Control, 2016
A methodology has been proposed to estimate non-proportional viscous damping matrix of beams from... more A methodology has been proposed to estimate non-proportional viscous damping matrix of beams from measured complex eigendata using finite element model updating technique. Representation of damping through a proportional damping matrix ignoring the complexity of eigenvectors may not be appropriate when external damping devices are employed. The current literature of determination of non-proportional damping matrix demands measurement of a large number of complex modes which is extremely difficult in practice. A gradient based finite element model updating algorithm implementing inverse eigensensitivity method has been presented through a series of numerically simulated cantilever beams. The method can accurately predict the non-proportional damping matrix even if the measured eigenvectors are polluted with random noise. The novelty of the current method is that it can sustain a high level of modal and coordinate sparsity in measurement. The method assumes prior determination or upda...
International Journal of Mechanical Sciences, 2017
American Journal of Plant Sciences, 2017