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Papers by Suleiman Isa
The African Journal of Pharmacology and Therapeutics, 2016
Background: Kigelia africana , belongs to the family of Bignoniaceae . It has been used commonly ... more Background: Kigelia africana , belongs to the family of Bignoniaceae . It has been used commonly in folk medicine to energise and improve fertility in both males and females. Decoctions of Kigelia africana are taken as abortifacients. Therefore, there is need to scientifically substantiate and validate these claims. Objective: This study was carried out to determine the effect of Kigelia africana on pregnancy and pregnancy outcomes in Sprague-Dawley rats. Methodology: Twenty female adult Sprague-Dawley rats divided into 4 groups (N=5) were used. Rats were mated on proestrus with males of proven fertility. Spermatocytes in the vaginal smear confirmed pregnancy. Kigelia africana was given at 100, 300 and 500 mg/kg bodyweight daily from the 1 st to 20 th day of pregnancy while control rats received distilled water. Rats were anaesthetized with ketamine on day 20 of pregnancy and a ventral laparotomy was performed. Foetuses were removed and parameters taken. The uterus was excised and a...
Lipids in Health and Disease, 2011
Background: In obesity, phenotypic switches occur in macrophage populations such that the predomi... more Background: In obesity, phenotypic switches occur in macrophage populations such that the predominantly M2-polarised anti-inflammatory state seen in lean individuals changes to a predominantly M1-polarised pro-inflammatory state in those who are obese. However, the mechanisms by which these phenotypic shifts occur have not yet been fully elucidated. Results: The effects of oxLDL (1-40 μg/ml; 24 h) on several parameters relevant to the Unfolded Protein Response (UPR)-mediated lipotoxic effects of oxLDL (disruption of ER Ca 2+ handling; activation of the UPR transcription factor XBP-1; upregulation of the UPR target genes BiP and CHOP; apoptosis; cell viability) were investigated in human primary monocyte-derived macrophages, and also in monocyte-macrophages derived from the THP-1 monocytic cell line. A consistent pattern was observed: M2-polarised macrophages were more sensitive to the lipotoxic effects of oxLDL than either non-polarised macrophages or non-differentiated monocytic cells. Specifically, M2-polarised macrophages were the only cell type to undergo significantly increased apoptosis (Primary cells: 1.23 ± 0.01 basal; THP-1-derived: 1.97 ± 0.12 basal; P < 0.05 in both cases) and decreased cell viability (Primary cells: 0.79 ± 0.04 basal; THP-1-derived: 0.67 ± 0.02 basal; P < 0.05 in both cases) when exposed to oxLDL levels similar to those seen in overweight individuals (ie. 1 μg/ml). Conclusions: We propose that the enhanced susceptibility of M2-polarised macrophages to lipotoxicity seen in the present in vitro study could, over time, contribute to the phenotypic shift seen in obese individuals in vivo. This is because a higher degree of oxLDL-induced lipotoxic cell death within M2 macrophages could contribute to a decrease in numbers of M2 cells, and thus a relative increase in proportion of non-M2 cells, within macrophage populations. Given the pro-inflammatory characteristics of a predominantly M1-polarised state, the data presented here may constitute a useful contribution to our understanding of the origin of the pro-inflammatory nature of obesity, and of the pathogenesis of obesity-associated inflammatory disorders such as Type 2 Diabetes and atherosclerosis.
The African Journal of Pharmacology and Therapeutics, 2016
Background: Kigelia africana , belongs to the family of Bignoniaceae . It has been used commonly ... more Background: Kigelia africana , belongs to the family of Bignoniaceae . It has been used commonly in folk medicine to energise and improve fertility in both males and females. Decoctions of Kigelia africana are taken as abortifacients. Therefore, there is need to scientifically substantiate and validate these claims. Objective: This study was carried out to determine the effect of Kigelia africana on pregnancy and pregnancy outcomes in Sprague-Dawley rats. Methodology: Twenty female adult Sprague-Dawley rats divided into 4 groups (N=5) were used. Rats were mated on proestrus with males of proven fertility. Spermatocytes in the vaginal smear confirmed pregnancy. Kigelia africana was given at 100, 300 and 500 mg/kg bodyweight daily from the 1 st to 20 th day of pregnancy while control rats received distilled water. Rats were anaesthetized with ketamine on day 20 of pregnancy and a ventral laparotomy was performed. Foetuses were removed and parameters taken. The uterus was excised and a...
Lipids in Health and Disease, 2011
Background: In obesity, phenotypic switches occur in macrophage populations such that the predomi... more Background: In obesity, phenotypic switches occur in macrophage populations such that the predominantly M2-polarised anti-inflammatory state seen in lean individuals changes to a predominantly M1-polarised pro-inflammatory state in those who are obese. However, the mechanisms by which these phenotypic shifts occur have not yet been fully elucidated. Results: The effects of oxLDL (1-40 μg/ml; 24 h) on several parameters relevant to the Unfolded Protein Response (UPR)-mediated lipotoxic effects of oxLDL (disruption of ER Ca 2+ handling; activation of the UPR transcription factor XBP-1; upregulation of the UPR target genes BiP and CHOP; apoptosis; cell viability) were investigated in human primary monocyte-derived macrophages, and also in monocyte-macrophages derived from the THP-1 monocytic cell line. A consistent pattern was observed: M2-polarised macrophages were more sensitive to the lipotoxic effects of oxLDL than either non-polarised macrophages or non-differentiated monocytic cells. Specifically, M2-polarised macrophages were the only cell type to undergo significantly increased apoptosis (Primary cells: 1.23 ± 0.01 basal; THP-1-derived: 1.97 ± 0.12 basal; P < 0.05 in both cases) and decreased cell viability (Primary cells: 0.79 ± 0.04 basal; THP-1-derived: 0.67 ± 0.02 basal; P < 0.05 in both cases) when exposed to oxLDL levels similar to those seen in overweight individuals (ie. 1 μg/ml). Conclusions: We propose that the enhanced susceptibility of M2-polarised macrophages to lipotoxicity seen in the present in vitro study could, over time, contribute to the phenotypic shift seen in obese individuals in vivo. This is because a higher degree of oxLDL-induced lipotoxic cell death within M2 macrophages could contribute to a decrease in numbers of M2 cells, and thus a relative increase in proportion of non-M2 cells, within macrophage populations. Given the pro-inflammatory characteristics of a predominantly M1-polarised state, the data presented here may constitute a useful contribution to our understanding of the origin of the pro-inflammatory nature of obesity, and of the pathogenesis of obesity-associated inflammatory disorders such as Type 2 Diabetes and atherosclerosis.