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Papers by Sumita banerjee
A bstract Dens evaginatus is a developmental anomaly of tooth characterized by presence of a tube... more A bstract Dens evaginatus is a developmental anomaly of tooth characterized by presence of a tubercle like projection from the tooth surface most commonly giving the form of a cusp. It comes under significant clinical consideration because of difficulty in diagnosis and having the chance of pulpal involvement after wearing off. The further consequences of a broken dens evaginatus can be pulp necrosis followed by formation of periapical pathology. There are several treatment options for this clinical entity but the treatment modality depends on the tubercle integrity, status of pulpal health and stage of root development of the involved tooth. Method: Here a case of broken dens evaginatus is reported with a large periapical lesion on both sides of mandibular first premolar having a wide open apex. For treatment, we opted the process of revascularization because it supports the physiological regeneration of periapical tissue and even helps in root completion. Result: At two years post...
Frontiers in Anti-infective Agents, 2019
International Journal of Applied Dental Sciences, 2017
Chlorhexidine Gluconate is a broad-spectrum antimicrobial drug that is widely used as mouthwash. ... more Chlorhexidine Gluconate is a broad-spectrum antimicrobial drug that is widely used as mouthwash. Several clinical studies have proved its safety as a mouthwash when used within pharmacological dose limit. However, severe cytotoxic effects on cultured cell line and even in animal trials also have been reported. Considering these findings, the rationality of the continuous use of Chlorhexidine Gluconate mouth wash for longer duration becomes questionable. To evaluate the genotoxicity of Chlorhexidine Gluconate for long term use within pharmacological dose limit by estimation of Micronucleus index in exfoliated human buccal mucosal cells using Acridine Orange under fluorescence microscope. In result no significant difference in the Micronuclei count in the whole population was observed before and after use of the 0.2% Chlorhexidine mouth wash. Micronucleus count was also unaltered in the male and female population before and after use.
Frontiers in Anti-infective Agents
Frontiers in Anti-infective Agents, 2019
Frontiers in Anti-infective Agents, 2019
Contemporary Clinical Dentistry
Background: Biomechanical preparation of root canal can damage root dentin leading to the formati... more Background: Biomechanical preparation of root canal can damage root dentin leading to the formation of dentinal cracks which can eventually lead to fracture and failure of the treatment. Aim: The aim was to investigate the incidence of dentinal defects in root canals prepared with ProTaper Universal, ProTaper Gold, and Hyflex electric discharge machining (EDM) rotary file systems using handheld USB digital microscope. Materials and Methods: One hundred and fifty extracted mandibular premolar teeth with single canal were randomly divided into five groups (n = 30). Group 1: unprepared (negative control). Group 2: canal preparation done with nickel–titanium hand files (positive control), Group 3: canals prepared with ProTaper Universal rotary system, Group 4: canal preparation done with ProTaper Gold rotary system, and Group 5: canals prepared with Hyflex EDM rotary system. The specimens were sliced at 3 mm, 6 mm, and 9 mm from the apex with a slow-speed saw under water cooling. Digital images of each section were captured at ×40 magnification with the aid of a microscope. Two independent evaluators assessed the images for the presence of dentinal defects. The number of dentinal defects was recorded and Chi-square test was used for statistical analysis (P < 0.05). Results: The number of specimens presenting dentinal defects was as follows: Group 3; ProTaper Universal – 9/30 (30.0%), Group 4; ProTaper Gold – 2/30 (6.7%), and Group 5; Hyflex EDM – 1/30 (3.3%). No defects were detected in the negative and positive control groups (Group 1 and Group 2). Conclusion: ProTaper Universal showed the highest percentage of defects in comparison to ProTaper Gold and Hyflex EDM.
International Journal of Oral Health Dentistry
International Journal of Oral Health Dentistry, Apr 15, 2018
The Kurume Medical Journal
Various endogenous mitochondria-associated antioxidants protect mitochondria from oxidative stres... more Various endogenous mitochondria-associated antioxidants protect mitochondria from oxidative stresses such as Superoxide Dismutase 2 (SOD2), Catalase, Glutaredoxin 2 (GLRX2), educed Glutathione (GSH), Glutathione Peroxidase (GPx), and Thioredoxin 2 (TXN2). They protect mitochondria from Reactive Oxygen Species (ROS). Excess ROS causes mitochondrial DNA damage and respiratory chain dysfunction leading to carcinogenesis. In an earlier study we found complex alterations of mitochondrial antioxidants in different stages of oral squamous cell carcinoma. Here, we profiled mitochondrial antioxidants in different oral potentially malignant disorders such as oral leukoplakia (OL), oral lichen planus (OLP), and oral submucous fibrosis (OSMF). Mitochondria was prepared from oral tissues from OL, OLP, and OSMF patients. Biochemical assays and immunoblotting were performed to investigate expression of various mitochondrial antioxidants. Catalase was expressed as Control> OL > OSMF > OLP. GLRX 2 was expressed as OLP> OL >Control >OSMF. GPX1 and GPX4 were expressed as Control >OSMF> OLP > OL. GSH increased in OL and OSMP, but decreased in OLP. SOD2 was expressed as Control >OSMF> OLP > OL. PRX3 was expressed as OL > OLP > OSMF > Control. TXN2 expression was nearly the same in all groups except OL, which showed elevated expression. We conclude that endogenous mitochondria-associated antioxidants show different levels of expressions in various oral potentially malignant disorders.
Journal of Advanced Clinical and Research Insights
Localized alveolar ridge defect in the partially edentulous maxillary anterior region creates an ... more Localized alveolar ridge defect in the partially edentulous maxillary anterior region creates an esthetic challenge for the restorative dentist. The formation of black triangle and residual space at the pontic ridge interface makes the prosthesis esthetically unpleasant and biologically unhealthy. Hence, the surgical correction of localized ridge defect has paramount importance before prosthetic replacement.
Journal of Advanced Clinical and Research Insights
Epithelial-mesenchymal transition, commonly known as EMT, plays the dominant role in the developm... more Epithelial-mesenchymal transition, commonly known as EMT, plays the dominant role in the developmental process, wound repair and tissue regeneration, and metastasis. EMT is classified into Type I, Type II, and Type III based on the molecular pathway it affects. Various molecular players such as transcription factors, growth factors, and cytokines play a significant role in the transition from epithelial cell type to mesenchymal cell type by change in the cell surface markers as well as the physiological changes such as increased mobility and invasiveness of the altered cell. In orofacial development, EMT plays a crucial role in organogenesis such as salivary gland development and tooth development. It is also contributory to palatogenesis.
Frontiers in Anti-infective Agents
Journal of Medicine, Radiology, Pathology and Surgery
Benign gingival overgrowths are not uncommon in the oral cavity. Reactive lesions and neoplasms c... more Benign gingival overgrowths are not uncommon in the oral cavity. Reactive lesions and neoplasms comprise the spectrum of local proliferations that occur in the gingiva. Similarity in the clinical features of these diverse lesions further compound the dilemma in arriving at a proper clinical diagnosis. Giant cell fibroma (GCF) is one such interesting lesion that may clinically mimic a fibroma or a papilloma that can only be diagnosed after histopathological examination from its unique and characteristic features. In this paper, the first case of GCF in an 18-year-old northeastern Indian of Mongoloid origin is reported for its rarity.
Journal of Oral and Maxillofacial Pathology, 2016
White sponge nevus (WSN) is an interesting hereditary oral mucosal disorder that commonly manifes... more White sponge nevus (WSN) is an interesting hereditary oral mucosal disorder that commonly manifests as bilaterally symmetrical, thickened white, corrugated or velvety, diffuse plaques that predominantly affects the buccal mucosa. The lesions may develop at birth or later in childhood or adolescence. Because it is asymptomatic and benign, WSN requires no treatment. Recognition of this disorder is important due to its potential confusion with other lesions that may be found in the oral cavity. Emphasis should be given to the early and correct diagnosis of this disorder to avoid unnecessary treatment. This report presents three affected members of a single family.
Journal of clinical and diagnostic research : JCDR, 2016
Preparation of good tissue specimens for microscopy requires complete fixation. No ideal fixative... more Preparation of good tissue specimens for microscopy requires complete fixation. No ideal fixative has been found till date, with every fixative showing advantages and disadvantages. Appropriate fixation is required to maintain clear and consistent morphologic features for histologic examination. Pathologists mostly examine formalin fixed tissue sections and are less used to the morphologic changes induced by other fixatives. Underfixed and overfixed tissue in various fixatives can lead to tissue architectural changes which can affect its diagnostic value. To assess sectioning ability, staining intensity and microscopic details of tissues kept in different fixatives at different time intervals. Fresh tissue specimen i.e., goat tongue was collected and its middle-third portion was used for the study purpose. The tissue was grossed into 10 equal pieces and kept in various fixatives (10% Buffered formalin, Carnoy's solution, Absolute ethyl alcohol, Bouin's fluid) for five differ...
Journal of clinical and diagnostic research : JCDR, 2016
Micronucleus (MN) is considered to be a reliable marker for genotoxic damage and it determines th... more Micronucleus (MN) is considered to be a reliable marker for genotoxic damage and it determines the presence and the extent of the chromosomal damage. The MN is formed due to DNA damage or chromosomal disarrangements. The MN has a close association with cancer incidences. In the new era, mobile phones are constantly gaining popularity specifically in the young generation, but this device uses radiofrequency radiation that may have a possible carcinogenic effect. The available reports related to the carcinogenic effect of mobile radiation on oral mucosa are contradictory. To explore the effects of mobile phone radiation on the MN frequency in oral mucosal cells. The subjects were divided into two major groups: low mobile phone users and high mobile phone users. Subjects who used their mobile phone since less than five years and less than three hours a week comprised of the first group and those who used their mobile since more than five years and more than 10 hours a week comprised of...
A bstract Dens evaginatus is a developmental anomaly of tooth characterized by presence of a tube... more A bstract Dens evaginatus is a developmental anomaly of tooth characterized by presence of a tubercle like projection from the tooth surface most commonly giving the form of a cusp. It comes under significant clinical consideration because of difficulty in diagnosis and having the chance of pulpal involvement after wearing off. The further consequences of a broken dens evaginatus can be pulp necrosis followed by formation of periapical pathology. There are several treatment options for this clinical entity but the treatment modality depends on the tubercle integrity, status of pulpal health and stage of root development of the involved tooth. Method: Here a case of broken dens evaginatus is reported with a large periapical lesion on both sides of mandibular first premolar having a wide open apex. For treatment, we opted the process of revascularization because it supports the physiological regeneration of periapical tissue and even helps in root completion. Result: At two years post...
Frontiers in Anti-infective Agents, 2019
International Journal of Applied Dental Sciences, 2017
Chlorhexidine Gluconate is a broad-spectrum antimicrobial drug that is widely used as mouthwash. ... more Chlorhexidine Gluconate is a broad-spectrum antimicrobial drug that is widely used as mouthwash. Several clinical studies have proved its safety as a mouthwash when used within pharmacological dose limit. However, severe cytotoxic effects on cultured cell line and even in animal trials also have been reported. Considering these findings, the rationality of the continuous use of Chlorhexidine Gluconate mouth wash for longer duration becomes questionable. To evaluate the genotoxicity of Chlorhexidine Gluconate for long term use within pharmacological dose limit by estimation of Micronucleus index in exfoliated human buccal mucosal cells using Acridine Orange under fluorescence microscope. In result no significant difference in the Micronuclei count in the whole population was observed before and after use of the 0.2% Chlorhexidine mouth wash. Micronucleus count was also unaltered in the male and female population before and after use.
Frontiers in Anti-infective Agents
Frontiers in Anti-infective Agents, 2019
Frontiers in Anti-infective Agents, 2019
Contemporary Clinical Dentistry
Background: Biomechanical preparation of root canal can damage root dentin leading to the formati... more Background: Biomechanical preparation of root canal can damage root dentin leading to the formation of dentinal cracks which can eventually lead to fracture and failure of the treatment. Aim: The aim was to investigate the incidence of dentinal defects in root canals prepared with ProTaper Universal, ProTaper Gold, and Hyflex electric discharge machining (EDM) rotary file systems using handheld USB digital microscope. Materials and Methods: One hundred and fifty extracted mandibular premolar teeth with single canal were randomly divided into five groups (n = 30). Group 1: unprepared (negative control). Group 2: canal preparation done with nickel–titanium hand files (positive control), Group 3: canals prepared with ProTaper Universal rotary system, Group 4: canal preparation done with ProTaper Gold rotary system, and Group 5: canals prepared with Hyflex EDM rotary system. The specimens were sliced at 3 mm, 6 mm, and 9 mm from the apex with a slow-speed saw under water cooling. Digital images of each section were captured at ×40 magnification with the aid of a microscope. Two independent evaluators assessed the images for the presence of dentinal defects. The number of dentinal defects was recorded and Chi-square test was used for statistical analysis (P < 0.05). Results: The number of specimens presenting dentinal defects was as follows: Group 3; ProTaper Universal – 9/30 (30.0%), Group 4; ProTaper Gold – 2/30 (6.7%), and Group 5; Hyflex EDM – 1/30 (3.3%). No defects were detected in the negative and positive control groups (Group 1 and Group 2). Conclusion: ProTaper Universal showed the highest percentage of defects in comparison to ProTaper Gold and Hyflex EDM.
International Journal of Oral Health Dentistry
International Journal of Oral Health Dentistry, Apr 15, 2018
The Kurume Medical Journal
Various endogenous mitochondria-associated antioxidants protect mitochondria from oxidative stres... more Various endogenous mitochondria-associated antioxidants protect mitochondria from oxidative stresses such as Superoxide Dismutase 2 (SOD2), Catalase, Glutaredoxin 2 (GLRX2), educed Glutathione (GSH), Glutathione Peroxidase (GPx), and Thioredoxin 2 (TXN2). They protect mitochondria from Reactive Oxygen Species (ROS). Excess ROS causes mitochondrial DNA damage and respiratory chain dysfunction leading to carcinogenesis. In an earlier study we found complex alterations of mitochondrial antioxidants in different stages of oral squamous cell carcinoma. Here, we profiled mitochondrial antioxidants in different oral potentially malignant disorders such as oral leukoplakia (OL), oral lichen planus (OLP), and oral submucous fibrosis (OSMF). Mitochondria was prepared from oral tissues from OL, OLP, and OSMF patients. Biochemical assays and immunoblotting were performed to investigate expression of various mitochondrial antioxidants. Catalase was expressed as Control> OL > OSMF > OLP. GLRX 2 was expressed as OLP> OL >Control >OSMF. GPX1 and GPX4 were expressed as Control >OSMF> OLP > OL. GSH increased in OL and OSMP, but decreased in OLP. SOD2 was expressed as Control >OSMF> OLP > OL. PRX3 was expressed as OL > OLP > OSMF > Control. TXN2 expression was nearly the same in all groups except OL, which showed elevated expression. We conclude that endogenous mitochondria-associated antioxidants show different levels of expressions in various oral potentially malignant disorders.
Journal of Advanced Clinical and Research Insights
Localized alveolar ridge defect in the partially edentulous maxillary anterior region creates an ... more Localized alveolar ridge defect in the partially edentulous maxillary anterior region creates an esthetic challenge for the restorative dentist. The formation of black triangle and residual space at the pontic ridge interface makes the prosthesis esthetically unpleasant and biologically unhealthy. Hence, the surgical correction of localized ridge defect has paramount importance before prosthetic replacement.
Journal of Advanced Clinical and Research Insights
Epithelial-mesenchymal transition, commonly known as EMT, plays the dominant role in the developm... more Epithelial-mesenchymal transition, commonly known as EMT, plays the dominant role in the developmental process, wound repair and tissue regeneration, and metastasis. EMT is classified into Type I, Type II, and Type III based on the molecular pathway it affects. Various molecular players such as transcription factors, growth factors, and cytokines play a significant role in the transition from epithelial cell type to mesenchymal cell type by change in the cell surface markers as well as the physiological changes such as increased mobility and invasiveness of the altered cell. In orofacial development, EMT plays a crucial role in organogenesis such as salivary gland development and tooth development. It is also contributory to palatogenesis.
Frontiers in Anti-infective Agents
Journal of Medicine, Radiology, Pathology and Surgery
Benign gingival overgrowths are not uncommon in the oral cavity. Reactive lesions and neoplasms c... more Benign gingival overgrowths are not uncommon in the oral cavity. Reactive lesions and neoplasms comprise the spectrum of local proliferations that occur in the gingiva. Similarity in the clinical features of these diverse lesions further compound the dilemma in arriving at a proper clinical diagnosis. Giant cell fibroma (GCF) is one such interesting lesion that may clinically mimic a fibroma or a papilloma that can only be diagnosed after histopathological examination from its unique and characteristic features. In this paper, the first case of GCF in an 18-year-old northeastern Indian of Mongoloid origin is reported for its rarity.
Journal of Oral and Maxillofacial Pathology, 2016
White sponge nevus (WSN) is an interesting hereditary oral mucosal disorder that commonly manifes... more White sponge nevus (WSN) is an interesting hereditary oral mucosal disorder that commonly manifests as bilaterally symmetrical, thickened white, corrugated or velvety, diffuse plaques that predominantly affects the buccal mucosa. The lesions may develop at birth or later in childhood or adolescence. Because it is asymptomatic and benign, WSN requires no treatment. Recognition of this disorder is important due to its potential confusion with other lesions that may be found in the oral cavity. Emphasis should be given to the early and correct diagnosis of this disorder to avoid unnecessary treatment. This report presents three affected members of a single family.
Journal of clinical and diagnostic research : JCDR, 2016
Preparation of good tissue specimens for microscopy requires complete fixation. No ideal fixative... more Preparation of good tissue specimens for microscopy requires complete fixation. No ideal fixative has been found till date, with every fixative showing advantages and disadvantages. Appropriate fixation is required to maintain clear and consistent morphologic features for histologic examination. Pathologists mostly examine formalin fixed tissue sections and are less used to the morphologic changes induced by other fixatives. Underfixed and overfixed tissue in various fixatives can lead to tissue architectural changes which can affect its diagnostic value. To assess sectioning ability, staining intensity and microscopic details of tissues kept in different fixatives at different time intervals. Fresh tissue specimen i.e., goat tongue was collected and its middle-third portion was used for the study purpose. The tissue was grossed into 10 equal pieces and kept in various fixatives (10% Buffered formalin, Carnoy's solution, Absolute ethyl alcohol, Bouin's fluid) for five differ...
Journal of clinical and diagnostic research : JCDR, 2016
Micronucleus (MN) is considered to be a reliable marker for genotoxic damage and it determines th... more Micronucleus (MN) is considered to be a reliable marker for genotoxic damage and it determines the presence and the extent of the chromosomal damage. The MN is formed due to DNA damage or chromosomal disarrangements. The MN has a close association with cancer incidences. In the new era, mobile phones are constantly gaining popularity specifically in the young generation, but this device uses radiofrequency radiation that may have a possible carcinogenic effect. The available reports related to the carcinogenic effect of mobile radiation on oral mucosa are contradictory. To explore the effects of mobile phone radiation on the MN frequency in oral mucosal cells. The subjects were divided into two major groups: low mobile phone users and high mobile phone users. Subjects who used their mobile phone since less than five years and less than three hours a week comprised of the first group and those who used their mobile since more than five years and more than 10 hours a week comprised of...