Surajit Debnath - Academia.edu (original) (raw)
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Papers by Surajit Debnath
Computational and Mathematical Methods, 2019
In this article, we have considered a continuous tritrophic food chain model subject to the Allee... more In this article, we have considered a continuous tritrophic food chain model subject to the Allee effect on the prey growth function with prey-dependent Holling type II functional response between the prey and intermediate predator; Crowley-Martin senses functional response between intermediate predator and top predator, and the top predator is of sexually reproductive type. We have established the positivity and boundedness of the system and the condition of existence of different equilibrium points. The local and global stability of the solutions about the various equilibrium points has been investigated. The center manifold theorem has been used to find the nature of the solution in the neighborhood of the nonhyperbolic equilibrium points and the direction of Hopf bifurcations. Numerical simulation has been carried out to establish the theoretical findings and finally some concluding remarks are given.
SpringerPlus, 2014
Correlating antifungal Azole drug resistance and mis-sense mutations of ERG11 has been paradoxica... more Correlating antifungal Azole drug resistance and mis-sense mutations of ERG11 has been paradoxical in pathogenic yeast Candida albicans. Amino acid substitutions (single or multiple) are frequent on ERG11, a membrane bound enzyme of Ergosterol biosynthesis pathway. Presence or absence of mutations can not sufficiently predict susceptibility. To analyze role of mis-sense mutations on Azole resistance energetically optimized, structurally validated homology model of wild C.albicans ERG11 using eukaryotic template was generated. A Composite Search Approach is proposed to identify vital residues for interaction at 3D active site. Structural analysis of catalytic groove, dynamics of substrate access channels and proximity of Heme prosthetic group characterized ERG11 active site. Several mis-sense mutations of ERG11 reported in C.albicans clinical isolates were selected through a stringent criterion and modeled. ERG11 mutants subsequently subjected to a four tier comparative biophysical analysis. This study indicates (i) critical interactions occur with residues at anterior part of 3D catalytic groove and substitution of these vital residues alters local geometry causing considerable change in catalytic pocket dimension. (ii) Substitutions of vital residues lead to confirmed resistance in clinical isolates that may be resultant to changed geometry of catalytic pocket. (iii)These substitutions also impart significant energetic changes on C.albicans ERG11 and (iv) include detectable dynamic fluctuations on the mutants. (v)Mis-sense mutations on the vital residues of the active site and at the vicinity of Heme prosthetic group are less frequent compared to rest of the enzyme. This large scale mutational study can aid to characterize the mutants in clinical isolates.
Computational and Mathematical Methods, 2019
In this article, we have considered a continuous tritrophic food chain model subject to the Allee... more In this article, we have considered a continuous tritrophic food chain model subject to the Allee effect on the prey growth function with prey-dependent Holling type II functional response between the prey and intermediate predator; Crowley-Martin senses functional response between intermediate predator and top predator, and the top predator is of sexually reproductive type. We have established the positivity and boundedness of the system and the condition of existence of different equilibrium points. The local and global stability of the solutions about the various equilibrium points has been investigated. The center manifold theorem has been used to find the nature of the solution in the neighborhood of the nonhyperbolic equilibrium points and the direction of Hopf bifurcations. Numerical simulation has been carried out to establish the theoretical findings and finally some concluding remarks are given.
SpringerPlus, 2014
Correlating antifungal Azole drug resistance and mis-sense mutations of ERG11 has been paradoxica... more Correlating antifungal Azole drug resistance and mis-sense mutations of ERG11 has been paradoxical in pathogenic yeast Candida albicans. Amino acid substitutions (single or multiple) are frequent on ERG11, a membrane bound enzyme of Ergosterol biosynthesis pathway. Presence or absence of mutations can not sufficiently predict susceptibility. To analyze role of mis-sense mutations on Azole resistance energetically optimized, structurally validated homology model of wild C.albicans ERG11 using eukaryotic template was generated. A Composite Search Approach is proposed to identify vital residues for interaction at 3D active site. Structural analysis of catalytic groove, dynamics of substrate access channels and proximity of Heme prosthetic group characterized ERG11 active site. Several mis-sense mutations of ERG11 reported in C.albicans clinical isolates were selected through a stringent criterion and modeled. ERG11 mutants subsequently subjected to a four tier comparative biophysical analysis. This study indicates (i) critical interactions occur with residues at anterior part of 3D catalytic groove and substitution of these vital residues alters local geometry causing considerable change in catalytic pocket dimension. (ii) Substitutions of vital residues lead to confirmed resistance in clinical isolates that may be resultant to changed geometry of catalytic pocket. (iii)These substitutions also impart significant energetic changes on C.albicans ERG11 and (iv) include detectable dynamic fluctuations on the mutants. (v)Mis-sense mutations on the vital residues of the active site and at the vicinity of Heme prosthetic group are less frequent compared to rest of the enzyme. This large scale mutational study can aid to characterize the mutants in clinical isolates.