Rudee Surarit - Academia.edu (original) (raw)

Papers by Rudee Surarit

Walailak Procedia, Mar 1, 2018

Objective: This study aimed to evaluate the effect of chitosan-containing mouthrinse on de-remine... more Objective: This study aimed to evaluate the effect of chitosan-containing mouthrinse on de-remineralization process by evaluating surface microhardness (SMH) measurement. Method: 32 human premolars were cut and embedded in self-cure acrylic resin. After polishing, all samples with window4x4-mm 2 were immersed in the demineralizing solution to create artificial caries and then were randomly divided into 4 groups; deionized water as negative control, 0.5% of chitosan, 0.5% of chitosan + 0.05% NaF and 0.05% NaF mouthrinse. They were entered into a 10-day pH cycling twice daily treated with tested mouthrinse. The surface microhardness (SMH) was measured 3 times; at baseline, before and after pH cycling using the microhardness tester (FM700 Future Tech Corp. Japan). The percentages of ∆SMH were compared using One-way ANOVA and LSD test. Results: The percentages of ∆SMH in each group were 21.85±8.3, 34.94±15.1, 54.00±16.9, and 51.45±18.1VHN respectively. All treatment groups had significantly higher rehardening effects than the control group and no difference between the chitosan added NaF mouthrinse and NaF mouthrinse.(p<0.05) Conclusion: Chitosan mouthrinse showed a significant better effect on rehardening of enamel caries than negative control. However, the chitosan combined with NaF mouthrinse showed no difference effect from NaF mouthrinse.

PubMed, Sep 1, 2013

Continuous consumption of soft drinks is the main cause of potential oral health problems, includ... more Continuous consumption of soft drinks is the main cause of potential oral health problems, including dental caries and erosion. The purpose of this study was to compare the effect of three different types of soft drinks on the release of calcium from the enamel surface of teeth. Forty bovine teeth were selected for the experiment. They were divided into four groups (n=10/group): Group 1 (Coke), Group 2 (Pepsi), Group 3 (Sprite), and Group 4 (distilled water, the control). The pH of each beverage was measured using a pH meter. The release of calcium ions was measured using an atomic absorption spectrophotometer at baseline, 15, 30, and 60 minutes. The results were assessed by analysis of variance and then by the Tukey test (p< 0.05). Coke, with a pH of 2.39, was the most acidic among the soft drinks. Coke, Pepsi, and Sprite showed no significant mean differences in the calcium released, but there was a significant mean difference of these soft drinks with distilled water at 60 minutes. We concluded that prolonged exposure to soft drinks could lead to significant enamel loss.

PubMed, Dec 1, 2000

Previous studies revealed that interleukin-1beta (IL-1beta) was detectable in gingival crevicular... more Previous studies revealed that interleukin-1beta (IL-1beta) was detectable in gingival crevicular fluid (GCF) of patients with periodontitis, and the level was increased in level in gingival tissue extracts of active periodontal disease sites (defined as attachment loss > or = 2.5 mm over the preceding 2 months) compared to inactive sites or healthy sites. The present study evaluated the relationship of IL-1beta level in GCF and periodontal disease status. GCF was collected with Periopaper strips from 34 disease-active and 45 disease-inactive teeth in 11 untreated periodontitis patients and from 60 teeth in 15 healthy control subjects. Disease activity was defined as attachment loss of > or = 2.5 mm in at least one site of a tooth as determined by sequential probing. The absorbed GCF volume was determined using a Periotron 6000 and the crevicular IL-1beta level was determined using IL-1beta monoclonal antibody (Otsuka Pharmaceutical, Japan). IL-1beta was below the detection level of the assay (6 pg/ml) in the healthy control group but was detected in most teeth of the periodontitis group. However, disease-active teeth had higher IL-1beta level (Mann-Whitney U-test, p < 0.05) than disease-inactive teeth (mean total IL-1beta of 5.89 +/- 7.88 pg/tooth and 1.72 +/- 2.28 pg/tooth; mean concentration of 1.6 +/- 2.5 ng/ml and 0.6 +/- 0.83 ng/ml, respectively). The level of IL-1beta showed no correlation with probing depth, but had significant correlation (p < 0.05) with the extent of attachment loss. This study suggests that the level of IL-1beta in GCF may have a predictive value for determining active and inactive periodontal status.

Pediatric Dental Journal, Aug 1, 2020

Abstract Objective To evaluate the ability and acidogenicity of human breast milk (HBM) biofilm f... more Abstract Objective To evaluate the ability and acidogenicity of human breast milk (HBM) biofilm formation relative to other milk formulas. Materials and methods Pooled HBM from six donor mothers, plain milk-based formula, milk-based formula supplemented with sucrose, soy-based formula, and brain heart infusion (BHI) containing 10% sucrose were utilized for the evaluation of biofilm formation and its acidogenicity. Biofilms formed by these tested groups, with and without 105 CFU/ml of Streptococcus mutans, were stained with crystal violet that was quantified by measuring the absorbance value (OD) at 595 nm. The acidogenicity of the biofilms was assessed after a 24-hour incubation period. Statistical analysis Multivariate analysis, the Kruskal Wallis test, and the Bonferroni test were performed. A P-value less than 0.05 was considered to be statistically significant. Results HBM containing S. mutans had significantly less potential to form biofilms as compared to other milk formulas. The overall biofilm OD value was significantly lower in the HBM group as compared to the other groups (P Conclusions HBM tends to be non-cariogenic due to its low acidogenicity and limited biofilm formation.

The Journal of Nihon University School of Dentistry, 1993

Experiments were performed to determine if a pyrophosphate-containing anticalculus toothpaste cou... more Experiments were performed to determine if a pyrophosphate-containing anticalculus toothpaste could affect certain salivary constituents involved in the mineralization of dental plaque. The findings indicated no obvious changes in acid and alkaline pyrophosphatases, ionized calcium, total calcium, inorganic phosphate or pH in saliva for 1-135 min after brushing the teeth with the anticalculus toothpaste. Data obtained by brushing with the toothpaste three times daily for two weeks also confirmed these results. Our findings clearly indicate that pyrophosphate-stabilizing agents in the anticalculus toothpaste are not fully effective in the oral cavity. In addition, the pyrophosphate-containing toothpaste has no influence on the state of calcium and phosphate in saliva.

Photomedicine and Laser Surgery, Dec 1, 2010

Objective: This study aimed to investigate the response of human dental pulp cells lying on a thi... more Objective: This study aimed to investigate the response of human dental pulp cells lying on a thin dentin disc to Er:YAG irradiation. Background Data: Er:YAG laser irradiation has been effectively used for tooth cavity preparation with minimal damage to the dental pulp tissue. However, study of its direct effect on pulp cells has been limited. Materials and Methods: Primary human dental pulp cells were cultured and allowed to grow on one side of 500-mm-thick dentin discs. An Er:YAG laser at output energies of a 120, 300, or 500 mJ/pulse with a repetition rate of 10 Hz was used to ablate the non-cell surface of the dentin disc for 10 s with cooling irrigation. Results: Twenty-four hours after laser irradiation, light and scanning electron micrographs revealed pulp cells with a normal fibroblastic morphology for the 120 and 300 mJ laser-treated groups. In the 500 mJ laser-treated group, many pyknotic cells with knob-like projections on the cell surface were mostly observed: the number of cells with normal morphology decreased compared to that of the other groups. However, the production of type I procollagen assessed by the enzyme immunoassay increased in the 500 mJ laser-treated group significantly (p < 0.001). Conclusion: The low-energy Er:YAG laser (120 and 300 mJ at 10 Hz) with coolant irrigation did not cause damage to dental pulp cells at critical thickness (500 mm) of dentin, whereas the laser of 500 mJ at 10 Hz induced greater production of type I procollagen with partial damage to the cells.

Biochemical Journal, Nov 1, 1980

Several techniques have been used to demonstrate that the binding of specific ligands to human pl... more Several techniques have been used to demonstrate that the binding of specific ligands to human plasma vitamin D binding protein induces a change in protein conformation. Apoprotein and holoprotein show circular dichroism spectra of similar form in the peptide region with double minima at 207 and 218nm. The minimum mean residue ellipticity of apoprotein (20.6 x 103degrees. cm2dmol-1) is decreased by about 8% after vitamin D3 binding, suggesting a small change in the backbone conformation. Spectrofluorimetric studies showed that 25-hydroxycholecalciferol causes a saturable Abbreviations used: D3, vitamin D3 or cholecalciferol; Gc, group-specific component; SDS, sodium dodecyl sulphate; vitamin 1,25-(OH)2-D3, 1,25-dihydroxycholecalciferol; vitamin 25-(OH)-D3, 25-hydroxycholecalciferol.

Journal of Ethnopharmacology, Dec 1, 2006

Cryptolepis buchanani Roem. & Schult. (Asclepiadaceae), a climbing tree, is used as folk medicine... more Cryptolepis buchanani Roem. & Schult. (Asclepiadaceae), a climbing tree, is used as folk medicine in southeast Asia. In Thailand, the stem of this plant is traditionally used for the treatment of inflammation, including arthritis, and muscle and joint pain. In the current study, the potential anti-inflammatory activity of a 50% ethanol extract of this plant was evaluated in a number of experimental models. For anti-acute inflammatory activity, results showed that the extract caused reduction of carrageenan-induced rat paw edema in addition to significant reduction of eicosanoid production from calcium ionophore A23187-stimulated rat peritoneal leukocytes. In a test for anti-chronic inflammatory potential utilizing the cotton thread-induced granuloma, the extract caused significant lowering of granulation tissue formation. The reduction of tumor necrosis factor-␣ (TNF-␣) release from LPS-stimulated human monocytic cell line (THP-1) was also demonstrated in cells that were pre-incubated with the extract. An additional important feature of Cryptolepis buchanani is its low toxicity, especially by oral treatment, which significantly encourages clinical trials of this extract in the human. In conclusion, the results give scientific support to the traditional use of this plant for combating inflammation. Further investigations are required to identify the active constituents responsible for the anti-inflammatory activity of Cryptolepis buchanani. Subacute and chronic toxicological studies in animals are also needed before clinical trials.

Clinical Oral Investigations, Dec 16, 2016

Objectives The study aimed at assessing oxidative stress (OS) biomarker levels in the saliva of p... more Objectives The study aimed at assessing oxidative stress (OS) biomarker levels in the saliva of patients with chronic periodontitis (CP) and acute coronary syndrome (ACS) and establishing their correlation to periodontal parameters and markers for cardiovascular events. Materials and methods The present study enrolled 24 patients with ACS and CP (the ACSCP group), 24 patients with ACS only (the ACS group), 24 patients with CP only (the CP group), and 24 healthy controls. Plaque index (PI), gingival index, bleeding on probing, probing pocket depth (PPD), and clinical attachment loss were recorded. Markers for cardiovascular events included serum high sensitivity C-reactive protein (hsCRP) and plasma fibrinogen. 8-Hydroxydeoxyguanosine (8-OHdG), protein carbonyl (PC), malondialdehyde (MDA), and total antioxidant capacity (TAOC) were used as OS biomarkers. Results Salivary 8-OHdG, MDA, and PC levels were significantly higher in the ACSCP, ACS, and CP groups than in healthy controls (p < 0.05). There were significant correlations between salivary PC levels and PI or PPD (p < 0.05) as well as between salivary 8-OHdG levels and all periodontal parameters (p < 0.05). TAOC levels in saliva were correlated to both serum hsCRP and plasma fibrinogen (p < 0.05). Salivary MDA levels were correlated to all periodontal parameters and biomarkers for cardiovascular events (p < 0.05). Conclusions Salivary OS biomarker levels were higher in diseased groups compared to control. They also correlated to clinical periodontal parameters and markers for cardiovascular events in ACS patients, with or without CP. Clinical relevance Salivary OS biomarkers could potentially serve as diagnostic tools for cardiovascular and/or periodontal diseases.

Clinical Oral Investigations, Feb 15, 2023

Journal of Materials Science: Materials in Medicine, Sep 13, 2008

The aim of this study was to investigate the degree of deacetylation (DD) and molecular weight (M... more The aim of this study was to investigate the degree of deacetylation (DD) and molecular weight (MW) of chitosan within chitosan-collagen scaffolds on mouse osteoblasts (MC3T3-E1). The chitosan-collagen scaffolds were fabricated by freeze-drying technique. The studies on cell attachment and proliferation, alkaline phosphatase (ALP) activity, cell morphology, and mineralized nodule formation by osteoblasts on scaffolds were investigated. No statistically significant difference was found on cell attachment, but the chitosan-collagen scaffolds with low-DD chitosan had a statistically significantly (P &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.05) higher proliferative effect and ALP activity than those scaffolds with high-DD chitosan, regardless of molecular weight. Scanning electron images demonstrated that MC3T3-E1 cells grew well on all test scaffolds; on the contrary, mineralized nodule formation was not found. In conclusion, the DD of chitosan is a crucial factor for MC3T3-E1 cells and it should be considered in further applications for bone tissue engineering.

Biochemical and Biophysical Research Communications, Jun 1, 2012

Vasculogenic mimicry (VM) is the phenomenon where cancer cells mimic endothelial cells by forming... more Vasculogenic mimicry (VM) is the phenomenon where cancer cells mimic endothelial cells by forming blood vessels. A stem cell-like phenotype has been proposed to be involved in this tumor plasticity. VM seems to correlate with metastasis rate, but there have been no reports on the effects of pro-metastatic and pro-angiogenic factors or hepatocyte growth factor (HGF) and vascular endothelial growth factor (VEGF) on VM formation of hepatocellular carcinoma (HCC) cells. Here, we determine VM capacity and expression of stemness genes (Oct4, Sox2, Nanog and CD133) in well-and poorly-differentiated HCC cell lines. The poorly-differentiated cell line SK-Hep-1 with mesenchymal features (high invasiveness and expressing Vimentin, with no E-cadherin) could form VM in vitro, while the well-differentiated cell line HepG2 did not form VM. There was no correlation between expression of stemness genes and intrinsic VM capacity. However, HGF but not VEGF, could induce VM formation in HepG2, concomitant with epithelial-mesenchymal transition (EMT), de-differentiation and increased expression of stemness genes. Our results show that the role of stemness genes in VM capacity of HCC cells is likely to depend on differentiation status.

Molecular Oral Microbiology, Nov 8, 2017

Research Square (Research Square), Dec 9, 2022

Objectives The aim of this study was to evaluate the effect of various irrigation protocols in re... more Objectives The aim of this study was to evaluate the effect of various irrigation protocols in regenerative endodontic procedures (REPS) on the attachment, proliferation, migration, and differentiation of stem cells from the apical papilla (SCAPs). Materials and Methods Dentin specimens from 140 human third molars were irrigated with various protocols; group 1: normal sterile saline (NSS), group 2: EDTA, group 3: EDTA then 5 mL NSS, or group 4: EDTA then 20 mL NSS. The specimens were used in cell assays. For cell proliferation, SCAPs were seeded on dentin and the cell viability on days 1, 3, and 7 was determined using an MTT assay. At day 3, the attached cells' morphology was observed using SEM and cell migration was investigated using a Transwell-migration assay. The ALP activity and odonto/osteogenic differentiation gene expression was evaluated at day 7, 14, and 21 using an ALP activity assay and RT-qPCR. Results On day 3 and 7, group 4 demonstrated more viable cells than group 1 (p < 0.01). The amount of migrated cells in group 2, 3, and 4 was greater compared with group 1 (p < 0.05). Moreover, SCAP differentiation was similar between groups. Conclusions Irrigating dentin with EDTA alone or with EDTA then NSS promoted SCAP migration. However, a nal irrigation with 20 mL NSS after EDTA promoted SCAP proliferation without affecting their differentiation. Clinical relevance When using a blood clot as a scaffold, a nal ushing with 20 mL NSS after EDTA could be bene cial for clinical REP protocols.

Southeast Asian Journal of Tropical Medicine and Public Health, 2018

Southeast Asian Journal of Tropical Medicine and Public Health, 2019

Thai Journal of Veterinary Medicine, 2017

Periodontal diseases are the most common infectious diseases of dogs. Up to now, the knowledge ab... more Periodontal diseases are the most common infectious diseases of dogs. Up to now, the knowledge about periodontopathic bacteria in dogs remains sparse. The objectives of this study were to apply the Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis (PCR-DGGE) technique, cloning and 16S rRNA gene sequencing to the investigation into subgingival plaque bacteria from healthy and periodontitis dogs; and compare DGGE patterns from the subgingival plaque of dogs and humans. Sixty-eight bacterial species were detected from subgingival plaque of 12 Poodles in Thailand and the predominant genus was Porphyromonas (n=11). Porphyromonas gulae and Treponema denticola were the most common microflora in the periodontitis dog group. In addition, DGGE band patterns from the dogs' subgingival plaque were compared with those of humans' subgingival plaque. The DGGE results showed different bacterial community structures between the two species, which may influence pathogenesis. Therefore, because the pathogenesis of periodontal disease in dogs may not be the same as in human, employing dog as an animal model for studying this disease in human should be carefully analyzed.

European Journal of Dentistry, Jul 30, 2021

Objectives This study aimed to evaluate the effect of Porphyromonas gingivalis and nicotine on th... more Objectives This study aimed to evaluate the effect of Porphyromonas gingivalis and nicotine on the in vitro osteogenic differentiation of periodontal ligament (PDL) fibroblasts. Materials and Methods PDLs were cultured in Dulbecco's modified Eagle's medium containing 10% fetal bovine serum at 37°C under 5% CO 2 and 100% humidified atmosphere. Cells were incubated with various concentrations of nicotine and P. gingivalis extracts, and cell viability was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay. To study cell differentiation, PDLs (5 × 10 4 cells) were treated with the osteogenic differentiation medium containing 10 mM β-glycerophosphate, 10 nM dexamethasone, 50 mg/mL ascorbic acid, 1 μM nicotine, and 50 µg/mL P. gingivalis lysate. mRNA samples were collected at 0, 7, and 14 days. Odontogenic-related gene expression, namely, Runt-related transcription factor 2 (Runx2), collagen type I (COL1A1), and alkaline phosphatase (ALP) was determined by reverse transcription quantitative polymerase chain reaction (RT-qPCR). Calcified nodule formation was determined on day 28 using Alizarin Red S. Analysis of variance and Tukey's test were used to compare the difference among groups at significant level of p < 0.05. Results It showed that 50 µg/mL of P. gingivalis lysate and 1 µM of nicotine showed no toxicity to PDLs. Runx2, COL1A1, and ALP expression were found to decrease significantly after 7 days of treatment, while osteocalcin expression was found to decrease after 14 days. The nodule formation in the control group was much greater in both number and size of nodules than in experimental groups, which implied a positive sign of calcium deposition in controls. Conclusion The results indicated that nicotine and P. gingivalis showed adverse effect on osteogenic differentiation properties of PDLs.

Research Square (Research Square), Dec 6, 2022

Chitosan nanoparticles have long been used as antibacterial agent in several elds due to its adva... more Chitosan nanoparticles have long been used as antibacterial agent in several elds due to its advantages in physiochemical characteristics. This study aimed to evaluate the different chitosan sources and crosslinkers used for fabricating chitosan nanoparticles (CNs) and to consider the application of CNs as an antibacterial agent in mouthwash. Different CNs structures were synthesized and subsequently incorporated into four different mouthwash solutions. Particle size, polydispersity index (PDI) and zeta potential of CNs were evaluated. The stability of CNs was also observed at different pH (5.0, 5.5 and 6.0) for over 2 months. At the optimal pH condition, each mouthwash formulation with CNs was tested for their antimicrobial properties against two bacterial species frequently associated with dental caries; Streptococcus mutans and Lactobacillus casei. The results showed that CNs could be synthesized from both squid-and shrimp-derived chitosan materials. When CNs-TPP was resuspended in either stock buffer or mouthwash solution often have smaller particle size compared to CNs-HMP. Among four formulations, the squid CNs-TPP mouthwash was found to be the most stable in particle size ranging from pH 5.0 to 6.0 during the 2-month period. The antibacterial results showed that the CNs-TPP were more effective in killing both S. mutans and L. casei. Our ndings suggested that CNs have potential to be used as an antibacterial agent in the mouthwash formulation with high stability.

European Journal of Dentistry

Objective Chrysin is a hydroxylated flavonoid derived from “propolis or bee glue,” a natural prod... more Objective Chrysin is a hydroxylated flavonoid derived from “propolis or bee glue,” a natural product. Previous research on chrysin's biological functions, including anticancer activity, had been reported. However, chrysin's effect on oral squamous cell carcinoma (OSCC) is still scarce. This article aimed to test the cytotoxicity, antiproliferative, antimigration, anti-invasion, and apoptotic effects of purified chrysin in two OSCC cell lines, HSC4 and SCC25. Materials and Methods The malignant phenotype was assessed using cell proliferation, wound healing, and transwell assays. Cell apoptosis was determined using flow cytometry. The positive control was OSCC cells treated with cisplatin, and the negative control was OSCC cells incubated with 0.1% dimethyl sulfoxide. Results Chrysin at concentrations of 100 and 200 µM could inhibit OSCC cell proliferation, migration, and invasion, as well as enhance cell apoptosis, particularly in the early stages of apoptosis. Conclusion In ...