Dwi Suryanto - Academia.edu (original) (raw)
Papers by Dwi Suryanto
Biodiversitas Journal of Biological Diversity
Exploration of probiotics candidate have been accelerated from unique and novel source, one of wh... more Exploration of probiotics candidate have been accelerated from unique and novel source, one of which is from black soldier fly (Hermetia illucens). The black soldier flies are known as omnivores which are commonly reared, harvested and utilized to convert organic wastes into biomass yet reducing the amount of abundant waste in nature. Lactic acid bacteria (LAB) is one of beneficial gut symbionts which can be found across animal taxa, including insect, however, limited information is currently reported from H. illucens as a potential harboring insect. This study aimed to determine the probiotics candidate from the gut of H. illucens fed with different feeding substrates to improve the gut microbiota. The larvae were nourished with chicken manure, tofu solid wastes and vegetable wastes prior isolation of LAB. The LAB isolates were tested for their antagonistic activity against enteric pathogens, pH and bile salt tolerance, and adherence to chicken intestine (ileum). Based on the growth performance under physiological stresses and multivariate analysis using principal component analysis (PCA), four LAB isolates were designated as putative probiotics. The four isolates were Lactiplantibacillus. pentosus 5P2i1, Lacticaseibacillus. paracasei 5P2i5, Lactiplantibacillus. pentosus 5P2i9, and Lactiplantibacillus. plantarum 6P1i1 was then declared as novel strains that could be developed further as poultry probiotics.
Journal of Pharmaceutical and Sciences, Dec 31, 2021
Bacterial resistance to antibiotics causes the death rate to increase. In dealing with this probl... more Bacterial resistance to antibiotics causes the death rate to increase. In dealing with this problem, studies were carried out, one of which was by utilizing natural medicinal ingredients such as bawang sabrang (Eleutherine palmifolia Merr.) of the Iridaceae family. This study aims to determine the antibacterial activity of the residual fraction of the ethanolic extract of bawang sabrang bulbs (Eleutherine palmifolia Merr.) against gram-positive and gram-negative bacteria and to determine the class of chemical compounds. Extraction was carried out by maceration using ethanol as a solvent and continued by successive fractionation using n-hexane and ethylacetate solvents, so that the remaining fraction was obtained. Antibacterial activity testing was carried out in vitro using the agar diffusion method using metal rings at concentrations of 20 mg/ml, 10 mg/ml and 5 mg/ml against gram-positive bacteria (Bacillus pumilus ATCC 7061, Bacillus subtilis ATCC 6633, Methicillin-resistant Staphylococcus aureus (MRSA) ATCC 25923, Micrococcus luteus ATCC 9341, Propionibacterium acne ATCC 6918, Staphylococcus aureus ATCC 29737, Staphylococcus epidermidis ATCC 12228) and gram-negative bacteria (Klebsiella pneumoniae ATCC 10031, Escherichia coli ATCC 10536, Salmonella thypi ATCC 29213). The analysis of the chemical compound group of the residual fraction was carried out using a phytochemical screening technique. The residual fraction of the ethanolic extract of bawang sabrang bulbs had antibacterial activity on all the tested bacteria. The chemical compounds contained in the remaining fraction of the ethanolic extract of bawang sabrang bulbs are alkaloids, flavonoids, glycosides, saponins, anthraquinone glycosides, tannins, triterpenoids, and steroids.
International Journal of PharmTech Research, 2020
Keratinolyticbacteria of A. chrocoocum B4 was evaluated for its potential of antibacterial and an... more Keratinolyticbacteria of A. chrocoocum B4 was evaluated for its potential of antibacterial and antioxidant activity. Kirby-Bauer method was used to know antibacterial potential of B4 againtsStaphylococcus aureus, S. epidermidis, Bacillus cereus, Bacillus pumilus, Bacillus subtilis, Listeria monocytogenes, Proteus sp., enteropathogenicEscherichia coli, Enterobactersakazakii, and Salmonella enterica. Antioxydant test was done using DPPH radical scavenging activity assay with ascorbic acid as a controll. In this study, hydrolysate of pellet, dialysis, and fraction 25 of B4 keratinase purification of previous study was used for antibacterial and antioxidant test. The result showed that B4 hydrolysatesinhibited Gram positive pathogenic bacteria such as Staphylococcus aureus and Listeria monocytogenes, and Gram negative Enterobacter sakazakii. All hydrolysates showed to have antioxidant properties in which fraction 25 showed higher compared to that of others. This study showed poultry was...
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati, 2019
Nine isolates of Bacillus thuringiensis of North Sumatra have been isolated from soil and dead la... more Nine isolates of Bacillus thuringiensis of North Sumatra have been isolated from soil and dead larvae of Plutella xylostella. Bioassay of these isolates to larva of Heliothis armigera, Plutella xylostella, Aedes aegypti, and Culex sp. showed that the isolates have different spectrum and ability in controlling insect larvae. Interestingly, TU1 has similar ability to that of Dipel (B. thuringiensis var kurstaki strain HD-7) in controlling Dipteran (A. aegypti and Culex sp.) and Lepidopteran (H. armigera and P. xylostella). The others were not effective to Dipteran, but have various abilities to kill Lepidopteran. This result indicated that local isolates of B. thuringiensis of North Sumatra were varied in ability to kill different larvae.
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati, 2019
A Study on antimicrobial examination of fractions of methanol, ethyl-acetate, and n-hexane extrac... more A Study on antimicrobial examination of fractions of methanol, ethyl-acetate, and n-hexane extract of tabar-tabar (Costus speciosus) leaf to bacteria (Bacillus sp., Staphylococcus aureus, Escherichia coli, and Serratia marcescens) and yeast (Candida albicans), and their toxicity to brine-shrimp (Artemia salina) larvae has been done. Examination of antimicrobial activities to the microbes were done by agar diffusion method, while test for toxicity was done by exposing 2 days-old larvae to certain concentration of the extract. Preliminary tests on phytochemical compounds in leaf were done by Mayer’s, FeCl3, Mg-HCl, foam, and 10% NaOH in methanol tests. The results showed that methanol leaf extract of tabar-tabar in general inhibited more in the growth of gram-positive Bacillus sp. and S. aureus rather than to other tested microbes. Compared to methanol and ethyl-acetate fraction, n-hexane fraction of the leaf inhibited more Bacillus sp. and E. coli. Interestingly, C. albicans was high...
International Journal of ChemTech Research, 2018
A study on isolation of chitinolytic bacteria from two lizard digestive tract of tokay gecko (Gek... more A study on isolation of chitinolytic bacteria from two lizard digestive tract of tokay gecko (Gekko gecko)and golden skink (Mabouya multifasciata), and characterization of their crude chitinase has been done. Bacterial isolation was conducted on chitin medium incubated at 31 ̊C. Chitinolytic index was measured as ratio of clear zone diameter divided by colony diameter. Crude enzyme activity of the isolates was spectrophotometrically assayed using Schales reagent at 420 nm.Bacterial identification was conducted by using its 16S rRNA gene sequences. Twelvebacterial isolateswere isolated from digestive tract oftokay gekko and eight were isolated from digestive tractof golden skink.LK12 and KD5 showed to haverelatively highchitinolytic index of 1,551 and 1,098 respectively. Optimum enzyme activity of LK12 and KD5 was observed in 6 days of incubation with activity of 0,205 U/mL and 0,225 U/mL, respectively. Bacterial identification showed that LK12 and KD5 were closed to Stenothrophomona...
Jurnal Ilmiah PANNMED (Pharmacist, Analyst, Nurse, Nutrition, Midwivery, Environment, Dentist), 2018
Kulit kayu manis merupakan salah satu obat tradisional yang diduga mengandung beberapa senyawa an... more Kulit kayu manis merupakan salah satu obat tradisional yang diduga mengandung beberapa senyawa antibakteri seperti flavonoid, saponin dan cinnamaldehid yang dapat menghambat pertumbuhan bakteri di rongga mulut. Efek ekstrak kulit kayu manis secara in vitro terhadap bakteri di rongga mulut telah sering dilakukan namun pengaruhnya sebagai obat kumur dalam menurunkan indeks plak gigi perlu dikaji. Penelitian ini bertujuan untuk mengetahui efektivitas berkumur dengan larutan ekstrak etanol kulit kayu manis dalam menurunkan akumulasi plak gigi. Kulit kayu manis diekstrak secara maserasi dengan etanol 80%. Obat kumur kulit kayu manis dibuat pada konsentrasi 6.25%. Sampel dalam penelitian ini merupakan responden yang berkunjung di klinik Drg. Syahdiana Waty, Medan, Sumatera Utara. Pengukuran indeks plak gigi dilakukan dua kali yaitu sebelum berkumur dan sesudah berkumur dengan larutan ekstrak etanol kulit kayu manis. Analisis data yang digunakan adalah uji Wilcoxon untuk mengetahui perbeda...
International Journal of ChemTech Research, 2018
Bacillus thuringiensis(Bt) is a gram-positive bacteria, spore-forming, and producing crystal prot... more Bacillus thuringiensis(Bt) is a gram-positive bacteria, spore-forming, and producing crystal proteinsused as bioinsecticides. The purpose of the study was to know potential of local isolates of Bt in controlling mosquito larva of Aedes aegypti.Isolation of Bt was conducted from soil samples of Tongkoh Forest, Brastagi, North Sumatra, Indonesia. Bt spore and crystal protein were observed using compound light microscope and further using scanning electron microscope. Two suspected isolates were choosen based on their morphological and biochemical characteristic. Further identification was done using their 16S rRNA gene. To know Bt growth pattern in inexpensive C and N-source, Bt isolate was grown in culture media with molasses and urea as C and N sources. To assay on Bt isolate to control mosquito, larva instar 3 of mosquito were put in 50 ml of test media of concentration of 10, 20, 30, 40, and 50% of Bt culture in plastic cup.Observation was done after 24 hrs. Two isolates SP7 and S...
Journal of Food and Pharmaceutical Sciences, 2021
Food spoilage during storage occurs physically, chemically and biologically which is related to t... more Food spoilage during storage occurs physically, chemically and biologically which is related to the activity of bacteria. One of the natural preservation that is currently developing is the application of edible coating on perishable food, such as fishball. Addition of antibacterial agent is important to improve edible coating. Virgin coconut oil contains medium chain fatty acids which have antimicrobial properties, particularly monoglycerides and free fatty acids that produced by hydrolyzing partially triglycerides at the Sn-1 and Sn-3 position using Lipozyme. The aim of this research was to test the effect of edible coating carrageenan enriched with virgin coconut oil hydrolysis (HVCO) (concentrations 1%,3%,5%) on fishball quality.The method was an experimental with factorial completely randomized design.The samples were analyzed for sensorial assessments, Total plate Count, Total Volatile Base-Nitrogen, Water content, and pH for 5 days at room temperature. The study demonstrated ...
IOP Conference Series: Earth and Environmental Science, 2018
View the article online for updates and enhancements. Related content Pathotype profile of Xantho... more View the article online for updates and enhancements. Related content Pathotype profile of Xanthomonas oryzae pv. oryzae isolates from North Sumatera Z Noer, Hasanuddin, Lisnawita et al. Content from this work may be used under the terms of the Creative Commons Attribution 3.0 licence. Any further distribution of this work must maintain attribution to the author(s) and the title of the work, journal citation and DOI.
Talenta Conference Series: Tropical Medicine (TM), 2018
Fusobacterium nucleatum pada plak gigi berperan penting sebagai jembatan peralihan antara kolonis... more Fusobacterium nucleatum pada plak gigi berperan penting sebagai jembatan peralihan antara kolonisasi bakteri awal plak gigi dengan kolonisasi bakteri berikutnya, khususnya bakteri anaerob obligat. Fusobacterium nucleatum merupakan bakteri anaerob obligat, namun membran terluar F. nucleatum secara spesifik mengikat asam amino ke-21 dan 26 dari molekul staterin yang berasal dari saliva sehingga memungkinkan bakteri ini dapat tumbuh pada saliva dan plak supragingiva. Penelitian ini bertujuan untuk mengetahui keberadaan dan jumlah koloni F. nucleatum, konsentrasi staterin, korelasi keduanya pada saliva dan plak supragingiva. Penelitian observasional ini menggunakan desain cross- sectional pada 28 sampel Unstimulated whole saliva menggunakan metode spitting dan plak supragingiva matang dilekatkan pada paper point nomor 60 dari pasien di Instalasi Periodonsia RSGMP FKG USU. Konsentrasi staterin dihitung menggunakan ELISA, jumlah koloni F. nucleatum dengan mengkultur sampel pada media Crys...
Journal of Pure and Applied Microbiology, 2017
Wastewater of textile industry is considered as toxic waste containing various dyes and chemicals... more Wastewater of textile industry is considered as toxic waste containing various dyes and chemicals that makes it recalcitrants. Decolorization of wastewater of the industry was conducted in our laboratorium using fungi isolated from a local forest of North Sumatera. Six potential isolates, previously screened for the ability to grow in plate media containing 100% of wastewater, were used for the study in a liquid culture condition. The results show that all tested isolates grew. The growth response of isolates cultured in a media supplemeted with 1% glucose was about two to three times higher than that of cultures without glucose. In the first culture, the fungi grew on the surface of the media however in the latter experiment, it concentrated on the bottom of culture flask for all of tested isolates. Decolorization analysis shows that all isolates were able to decolorize the wastewater. The cultures supplemented with glucose decolorized wastewater from 20% to 46%, while cultures without glucose did much higher about 56% to 92%. Furthermore, a microscopic analysis shows that dyes molecules in the wastewater were absorbed to fungal mycelia. The findings show that extraceluler molecules existed on the surface of fungal mycelia play an important role in the absorption of dye molecules.
International Journal of PharmTech Research
Triglycerides and diglycerides do not show antibacterial activity, but free fatty acids and monog... more Triglycerides and diglycerides do not show antibacterial activity, but free fatty acids and monoglycerides, especially lauric acid and monolaurin do. The purpose of this study was to determine the antibacterial activity of the enzymatic hydrolyzed of virgin coconut oil (VCO) and palm kernel oil (PKO), which produce a combination of lauric acid and monolaurin. Hydrolysis process was done by the enzyme lipase (Lipozym TL IM) which was active at 1.3 position. The hydrolyzed oil then tested for antibacterial activity against Staphylococcus aureus, Escherichia coli and Salmonella thypi using agar diffusion method with paper disc with diameter of 6 mm (Oxoid, England). Antibacterial activity test carried out on VCO and PKO, hydrolyzed VCO (VCOH) and PKO (PKOH) each at 25% concentration, 50%, 75% and 100%. Antibacterial activity of the test material compared with chloramphenicol (30 µg) and tetracycline (30 µg). The results showed that the optimum hydrolysis time is 14 hours. There is low and similar antibacterial activity of VCO and PKO. VCOH and PKOH show higher antibacterial activity than VCO and PKO. There is an increase of antibacterial activity by increased levels of VCOH and PKOH. Antibacterial activity against S. aureus (Gram positive) was higher than S. thypi and E. coli (Gram negative). Antibacterial activity of the test material is lower than the standard chloramphenicol and tetracycline .
HAYATI Journal of Biosciences, 2013
Tangkahan Conservation Forest in Karo County, North Sumatera has high biodiversity of endemic her... more Tangkahan Conservation Forest in Karo County, North Sumatera has high biodiversity of endemic herbs. Many species of the wild herbs are well known used as traditional medicine not only by local people but also by people out of the area. The methanol extract of the medicinal wild herbs in Tangkahan Conservation Forest, Karo County to relief skin diseases caused by bacteria and fungi never been studied medically. The antimicrobial activity leave extract of the medicinal herbs to pathogenic microorganisms are studied. The leaves extract of kembu-kembu (Callicarpa candicans), rintih bulung (Piper muricatum), cep-cepan (Castanopsis costata), and sereh kayu (Eugenia grandis), has antimicrobial to bacteria (Bacillus sp., Escherichia coli, Serratia marcescens, Staphylococcus aureus) and yeast (Candida albicans). Toxicity assay of these plants by brine shrimp method using Artemia salina indicates that cep-cepan dan sereh kayu have lethal concentration higher than kembu-kembu and rintih bulung.
International Journal of ChemTech Research, 2019
A study on isolation and antibacterial activity of endophytic bacteria of gambir (Uncaria gambir ... more A study on isolation and antibacterial activity of endophytic bacteria of gambir (Uncaria gambir (Hunter) Roxb.), along with its antibacterial extract activity test on clinical pathogenic bacteria has been conducted. Isolation of gambir endophytic bacteria was done in nutrient agar incubated at ambient temperature for 48 hours. Samples of gambir leaves and root was used for source of endophytic bacteria. Bacterial isolate was propagated using Mueller Hinton Agar. Bacterial culture was extracted using methanol as solvent. To test methanol extract activity of bacterial isolates, concentration of 20, 40, 60, 80, and 100% (v/v) were used in paper disc. Pathogenic bacterial isolates such as Escherichia coli, Streptococcus mutans, and Staphylococcus aureus were used for antibacterial test. Inhibition zone formed around paper disc indicated antibacterial extract activity against the bacteria. Endophytic bacterial isolates of gambirwas identified using Vitek Compact 2.0® based on biochemica...
To propagate bacterial cell for biocontrol purpose, suitable nutrient have to be determined in wh... more To propagate bacterial cell for biocontrol purpose, suitable nutrient have to be determined in which carbon and nitrogen source was often as limited factor of bacterial growth. Proper storage for biocontrol agent such as bacterial cell should also be considered in order to keep the cell viable when used. The aim of this study is to find out suitable carbon and nitrogen sources for viability and swarming of chitinolitic bacterial Bacillus sp. BK17 and Enterobacter sp. BK15. The highest population of bacterial growth (3.7x10 8 cfu/ml) was found in molases-sodium nitrate (MS) medium and the lowest population was found in crab shell-sodium nitrate (CS) growth (2.4x10 8 cfu/ml) after 25 days of incubation. The swarming activity of the isolates were varied to some extent with the highest was 51 mm in 2% agar molases-urea after 5 days of incubation. Molases-sodium nitrate (MS) medium is suitable carbon and nitrogen source for the viability of Bacillus sp. and Enterobacter sp. Meanwhile, ag...
Bakteri patogen oportunistik merupakan bakteri yang secara alami bukan berada di habitat suatu li... more Bakteri patogen oportunistik merupakan bakteri yang secara alami bukan berada di habitat suatu lingkungan tetapi masuk akibat tercemarnya lingkungan tersebut dengan limbah manusia. Keberadaan bakteri patogen oportunistik di tambak udang disebabkan karena tercemarnya air tambak oleh limbah industri maupun domestik. Penelitian ini bertujuan untuk mengetahui keberadaan bakteri patogen oportunistik pada tambak udang di Sumatera Utara (Percut, Pantai Labu, Pantai Cermin). Bakteri patogen oportunistik diisolasi dari usus udang, sedimen dan air tambak udang dengan menggunakan media selektif/diferensial. Dari hasil isolasi ditemukan adanya bakteri Escherichia coli, Salmonella sp., dan Staphylococcus sp. Jumlah tertinggi dari ketiga bakteri tersebut ditemukan pada sampel yang berasal dari daerah Percut.
Journal of Pure and Applied Microbiology, 2020
Keratinase is a proteolytic enzyme capable on degrading the hardy polymeric biomolecule or kerati... more Keratinase is a proteolytic enzyme capable on degrading the hardy polymeric biomolecule or keratin. In recent days, the utilization of keratinolytic microorganisms is seen as a promising way in recycling the keratin wastes from the avian and mammalians into valuable derived products. Previous study has reported the presence of a keratinolytic bacterium, later identified as Azotobacter chroococcum B4 obtained from dump soils. The present study investigates the enzyme characteristics of keratinase produced by this strain based on the physical appearance of final degraded product using SEM, the molecular weight of keratinase using SDS-PAGE, the effects of nutrition (C/N-source) on strain production of keratinase and the enzyme stability in metal ions solution. The molecular weight of keratinase produced by A. chroococcum B4 was about 30 kDa. Both sucrose and tryptone supplementation increase the keratinase activity by 71.7 and 97.8 U/mL after 96 h of cultivation. Metal ions, Ca 2+ , Mg 2+ , Mn 2+ , Na + , and K + are regarded as activators by increasing the relative activity of keratinase by 117, 166, 111, 113, and 112% respectively, while phenylmethylsulfonyl flouride (PMSF) is regarded as inhibitor by decreasing the relative activity down to 31%. Based on the metal ion characteristics, this strain produced a serine-protease type of keratinase which may further studied for its application in the field.
Asian Journal of Pharmaceutical and Clinical Research, 2018
Objective: This study was to analyze the karamunting leaf extract and fractions effectiveness ag... more Objective: This study was to analyze the karamunting leaf extract and fractions effectiveness against Staphylococcus aureus and Pseudomonas aeruginosa infection.Methods: Phytochemical screening is done in the extract and fractions of karamunting leaf continue with Standard agar well diffusion method for S. aureus and P. aeruginosa. Dimethyl sulfoxide was used as negative control, and standard antibiotic Kalmicetine (chloramphenicol) was used as positive control. Result: The ethyl acetate fraction showed the highest antibacterial activity against S. aureus and P. aeruginosa. Conclusion: Karamunting leaf can be used to treat the bacterial infection diseases especially infection from S. aureus and P. aeruginosa.
IOP Conference Series: Earth and Environmental Science, 2018
View the article online for updates and enhancements. Content from this work may be used under th... more View the article online for updates and enhancements. Content from this work may be used under the terms of the Creative Commons Attribution 3.0 licence. Any further distribution of this work must maintain attribution to the author(s) and the title of the work, journal citation and DOI.
Biodiversitas Journal of Biological Diversity
Exploration of probiotics candidate have been accelerated from unique and novel source, one of wh... more Exploration of probiotics candidate have been accelerated from unique and novel source, one of which is from black soldier fly (Hermetia illucens). The black soldier flies are known as omnivores which are commonly reared, harvested and utilized to convert organic wastes into biomass yet reducing the amount of abundant waste in nature. Lactic acid bacteria (LAB) is one of beneficial gut symbionts which can be found across animal taxa, including insect, however, limited information is currently reported from H. illucens as a potential harboring insect. This study aimed to determine the probiotics candidate from the gut of H. illucens fed with different feeding substrates to improve the gut microbiota. The larvae were nourished with chicken manure, tofu solid wastes and vegetable wastes prior isolation of LAB. The LAB isolates were tested for their antagonistic activity against enteric pathogens, pH and bile salt tolerance, and adherence to chicken intestine (ileum). Based on the growth performance under physiological stresses and multivariate analysis using principal component analysis (PCA), four LAB isolates were designated as putative probiotics. The four isolates were Lactiplantibacillus. pentosus 5P2i1, Lacticaseibacillus. paracasei 5P2i5, Lactiplantibacillus. pentosus 5P2i9, and Lactiplantibacillus. plantarum 6P1i1 was then declared as novel strains that could be developed further as poultry probiotics.
Journal of Pharmaceutical and Sciences, Dec 31, 2021
Bacterial resistance to antibiotics causes the death rate to increase. In dealing with this probl... more Bacterial resistance to antibiotics causes the death rate to increase. In dealing with this problem, studies were carried out, one of which was by utilizing natural medicinal ingredients such as bawang sabrang (Eleutherine palmifolia Merr.) of the Iridaceae family. This study aims to determine the antibacterial activity of the residual fraction of the ethanolic extract of bawang sabrang bulbs (Eleutherine palmifolia Merr.) against gram-positive and gram-negative bacteria and to determine the class of chemical compounds. Extraction was carried out by maceration using ethanol as a solvent and continued by successive fractionation using n-hexane and ethylacetate solvents, so that the remaining fraction was obtained. Antibacterial activity testing was carried out in vitro using the agar diffusion method using metal rings at concentrations of 20 mg/ml, 10 mg/ml and 5 mg/ml against gram-positive bacteria (Bacillus pumilus ATCC 7061, Bacillus subtilis ATCC 6633, Methicillin-resistant Staphylococcus aureus (MRSA) ATCC 25923, Micrococcus luteus ATCC 9341, Propionibacterium acne ATCC 6918, Staphylococcus aureus ATCC 29737, Staphylococcus epidermidis ATCC 12228) and gram-negative bacteria (Klebsiella pneumoniae ATCC 10031, Escherichia coli ATCC 10536, Salmonella thypi ATCC 29213). The analysis of the chemical compound group of the residual fraction was carried out using a phytochemical screening technique. The residual fraction of the ethanolic extract of bawang sabrang bulbs had antibacterial activity on all the tested bacteria. The chemical compounds contained in the remaining fraction of the ethanolic extract of bawang sabrang bulbs are alkaloids, flavonoids, glycosides, saponins, anthraquinone glycosides, tannins, triterpenoids, and steroids.
International Journal of PharmTech Research, 2020
Keratinolyticbacteria of A. chrocoocum B4 was evaluated for its potential of antibacterial and an... more Keratinolyticbacteria of A. chrocoocum B4 was evaluated for its potential of antibacterial and antioxidant activity. Kirby-Bauer method was used to know antibacterial potential of B4 againtsStaphylococcus aureus, S. epidermidis, Bacillus cereus, Bacillus pumilus, Bacillus subtilis, Listeria monocytogenes, Proteus sp., enteropathogenicEscherichia coli, Enterobactersakazakii, and Salmonella enterica. Antioxydant test was done using DPPH radical scavenging activity assay with ascorbic acid as a controll. In this study, hydrolysate of pellet, dialysis, and fraction 25 of B4 keratinase purification of previous study was used for antibacterial and antioxidant test. The result showed that B4 hydrolysatesinhibited Gram positive pathogenic bacteria such as Staphylococcus aureus and Listeria monocytogenes, and Gram negative Enterobacter sakazakii. All hydrolysates showed to have antioxidant properties in which fraction 25 showed higher compared to that of others. This study showed poultry was...
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati, 2019
Nine isolates of Bacillus thuringiensis of North Sumatra have been isolated from soil and dead la... more Nine isolates of Bacillus thuringiensis of North Sumatra have been isolated from soil and dead larvae of Plutella xylostella. Bioassay of these isolates to larva of Heliothis armigera, Plutella xylostella, Aedes aegypti, and Culex sp. showed that the isolates have different spectrum and ability in controlling insect larvae. Interestingly, TU1 has similar ability to that of Dipel (B. thuringiensis var kurstaki strain HD-7) in controlling Dipteran (A. aegypti and Culex sp.) and Lepidopteran (H. armigera and P. xylostella). The others were not effective to Dipteran, but have various abilities to kill Lepidopteran. This result indicated that local isolates of B. thuringiensis of North Sumatra were varied in ability to kill different larvae.
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati, 2019
A Study on antimicrobial examination of fractions of methanol, ethyl-acetate, and n-hexane extrac... more A Study on antimicrobial examination of fractions of methanol, ethyl-acetate, and n-hexane extract of tabar-tabar (Costus speciosus) leaf to bacteria (Bacillus sp., Staphylococcus aureus, Escherichia coli, and Serratia marcescens) and yeast (Candida albicans), and their toxicity to brine-shrimp (Artemia salina) larvae has been done. Examination of antimicrobial activities to the microbes were done by agar diffusion method, while test for toxicity was done by exposing 2 days-old larvae to certain concentration of the extract. Preliminary tests on phytochemical compounds in leaf were done by Mayer’s, FeCl3, Mg-HCl, foam, and 10% NaOH in methanol tests. The results showed that methanol leaf extract of tabar-tabar in general inhibited more in the growth of gram-positive Bacillus sp. and S. aureus rather than to other tested microbes. Compared to methanol and ethyl-acetate fraction, n-hexane fraction of the leaf inhibited more Bacillus sp. and E. coli. Interestingly, C. albicans was high...
International Journal of ChemTech Research, 2018
A study on isolation of chitinolytic bacteria from two lizard digestive tract of tokay gecko (Gek... more A study on isolation of chitinolytic bacteria from two lizard digestive tract of tokay gecko (Gekko gecko)and golden skink (Mabouya multifasciata), and characterization of their crude chitinase has been done. Bacterial isolation was conducted on chitin medium incubated at 31 ̊C. Chitinolytic index was measured as ratio of clear zone diameter divided by colony diameter. Crude enzyme activity of the isolates was spectrophotometrically assayed using Schales reagent at 420 nm.Bacterial identification was conducted by using its 16S rRNA gene sequences. Twelvebacterial isolateswere isolated from digestive tract oftokay gekko and eight were isolated from digestive tractof golden skink.LK12 and KD5 showed to haverelatively highchitinolytic index of 1,551 and 1,098 respectively. Optimum enzyme activity of LK12 and KD5 was observed in 6 days of incubation with activity of 0,205 U/mL and 0,225 U/mL, respectively. Bacterial identification showed that LK12 and KD5 were closed to Stenothrophomona...
Jurnal Ilmiah PANNMED (Pharmacist, Analyst, Nurse, Nutrition, Midwivery, Environment, Dentist), 2018
Kulit kayu manis merupakan salah satu obat tradisional yang diduga mengandung beberapa senyawa an... more Kulit kayu manis merupakan salah satu obat tradisional yang diduga mengandung beberapa senyawa antibakteri seperti flavonoid, saponin dan cinnamaldehid yang dapat menghambat pertumbuhan bakteri di rongga mulut. Efek ekstrak kulit kayu manis secara in vitro terhadap bakteri di rongga mulut telah sering dilakukan namun pengaruhnya sebagai obat kumur dalam menurunkan indeks plak gigi perlu dikaji. Penelitian ini bertujuan untuk mengetahui efektivitas berkumur dengan larutan ekstrak etanol kulit kayu manis dalam menurunkan akumulasi plak gigi. Kulit kayu manis diekstrak secara maserasi dengan etanol 80%. Obat kumur kulit kayu manis dibuat pada konsentrasi 6.25%. Sampel dalam penelitian ini merupakan responden yang berkunjung di klinik Drg. Syahdiana Waty, Medan, Sumatera Utara. Pengukuran indeks plak gigi dilakukan dua kali yaitu sebelum berkumur dan sesudah berkumur dengan larutan ekstrak etanol kulit kayu manis. Analisis data yang digunakan adalah uji Wilcoxon untuk mengetahui perbeda...
International Journal of ChemTech Research, 2018
Bacillus thuringiensis(Bt) is a gram-positive bacteria, spore-forming, and producing crystal prot... more Bacillus thuringiensis(Bt) is a gram-positive bacteria, spore-forming, and producing crystal proteinsused as bioinsecticides. The purpose of the study was to know potential of local isolates of Bt in controlling mosquito larva of Aedes aegypti.Isolation of Bt was conducted from soil samples of Tongkoh Forest, Brastagi, North Sumatra, Indonesia. Bt spore and crystal protein were observed using compound light microscope and further using scanning electron microscope. Two suspected isolates were choosen based on their morphological and biochemical characteristic. Further identification was done using their 16S rRNA gene. To know Bt growth pattern in inexpensive C and N-source, Bt isolate was grown in culture media with molasses and urea as C and N sources. To assay on Bt isolate to control mosquito, larva instar 3 of mosquito were put in 50 ml of test media of concentration of 10, 20, 30, 40, and 50% of Bt culture in plastic cup.Observation was done after 24 hrs. Two isolates SP7 and S...
Journal of Food and Pharmaceutical Sciences, 2021
Food spoilage during storage occurs physically, chemically and biologically which is related to t... more Food spoilage during storage occurs physically, chemically and biologically which is related to the activity of bacteria. One of the natural preservation that is currently developing is the application of edible coating on perishable food, such as fishball. Addition of antibacterial agent is important to improve edible coating. Virgin coconut oil contains medium chain fatty acids which have antimicrobial properties, particularly monoglycerides and free fatty acids that produced by hydrolyzing partially triglycerides at the Sn-1 and Sn-3 position using Lipozyme. The aim of this research was to test the effect of edible coating carrageenan enriched with virgin coconut oil hydrolysis (HVCO) (concentrations 1%,3%,5%) on fishball quality.The method was an experimental with factorial completely randomized design.The samples were analyzed for sensorial assessments, Total plate Count, Total Volatile Base-Nitrogen, Water content, and pH for 5 days at room temperature. The study demonstrated ...
IOP Conference Series: Earth and Environmental Science, 2018
View the article online for updates and enhancements. Related content Pathotype profile of Xantho... more View the article online for updates and enhancements. Related content Pathotype profile of Xanthomonas oryzae pv. oryzae isolates from North Sumatera Z Noer, Hasanuddin, Lisnawita et al. Content from this work may be used under the terms of the Creative Commons Attribution 3.0 licence. Any further distribution of this work must maintain attribution to the author(s) and the title of the work, journal citation and DOI.
Talenta Conference Series: Tropical Medicine (TM), 2018
Fusobacterium nucleatum pada plak gigi berperan penting sebagai jembatan peralihan antara kolonis... more Fusobacterium nucleatum pada plak gigi berperan penting sebagai jembatan peralihan antara kolonisasi bakteri awal plak gigi dengan kolonisasi bakteri berikutnya, khususnya bakteri anaerob obligat. Fusobacterium nucleatum merupakan bakteri anaerob obligat, namun membran terluar F. nucleatum secara spesifik mengikat asam amino ke-21 dan 26 dari molekul staterin yang berasal dari saliva sehingga memungkinkan bakteri ini dapat tumbuh pada saliva dan plak supragingiva. Penelitian ini bertujuan untuk mengetahui keberadaan dan jumlah koloni F. nucleatum, konsentrasi staterin, korelasi keduanya pada saliva dan plak supragingiva. Penelitian observasional ini menggunakan desain cross- sectional pada 28 sampel Unstimulated whole saliva menggunakan metode spitting dan plak supragingiva matang dilekatkan pada paper point nomor 60 dari pasien di Instalasi Periodonsia RSGMP FKG USU. Konsentrasi staterin dihitung menggunakan ELISA, jumlah koloni F. nucleatum dengan mengkultur sampel pada media Crys...
Journal of Pure and Applied Microbiology, 2017
Wastewater of textile industry is considered as toxic waste containing various dyes and chemicals... more Wastewater of textile industry is considered as toxic waste containing various dyes and chemicals that makes it recalcitrants. Decolorization of wastewater of the industry was conducted in our laboratorium using fungi isolated from a local forest of North Sumatera. Six potential isolates, previously screened for the ability to grow in plate media containing 100% of wastewater, were used for the study in a liquid culture condition. The results show that all tested isolates grew. The growth response of isolates cultured in a media supplemeted with 1% glucose was about two to three times higher than that of cultures without glucose. In the first culture, the fungi grew on the surface of the media however in the latter experiment, it concentrated on the bottom of culture flask for all of tested isolates. Decolorization analysis shows that all isolates were able to decolorize the wastewater. The cultures supplemented with glucose decolorized wastewater from 20% to 46%, while cultures without glucose did much higher about 56% to 92%. Furthermore, a microscopic analysis shows that dyes molecules in the wastewater were absorbed to fungal mycelia. The findings show that extraceluler molecules existed on the surface of fungal mycelia play an important role in the absorption of dye molecules.
International Journal of PharmTech Research
Triglycerides and diglycerides do not show antibacterial activity, but free fatty acids and monog... more Triglycerides and diglycerides do not show antibacterial activity, but free fatty acids and monoglycerides, especially lauric acid and monolaurin do. The purpose of this study was to determine the antibacterial activity of the enzymatic hydrolyzed of virgin coconut oil (VCO) and palm kernel oil (PKO), which produce a combination of lauric acid and monolaurin. Hydrolysis process was done by the enzyme lipase (Lipozym TL IM) which was active at 1.3 position. The hydrolyzed oil then tested for antibacterial activity against Staphylococcus aureus, Escherichia coli and Salmonella thypi using agar diffusion method with paper disc with diameter of 6 mm (Oxoid, England). Antibacterial activity test carried out on VCO and PKO, hydrolyzed VCO (VCOH) and PKO (PKOH) each at 25% concentration, 50%, 75% and 100%. Antibacterial activity of the test material compared with chloramphenicol (30 µg) and tetracycline (30 µg). The results showed that the optimum hydrolysis time is 14 hours. There is low and similar antibacterial activity of VCO and PKO. VCOH and PKOH show higher antibacterial activity than VCO and PKO. There is an increase of antibacterial activity by increased levels of VCOH and PKOH. Antibacterial activity against S. aureus (Gram positive) was higher than S. thypi and E. coli (Gram negative). Antibacterial activity of the test material is lower than the standard chloramphenicol and tetracycline .
HAYATI Journal of Biosciences, 2013
Tangkahan Conservation Forest in Karo County, North Sumatera has high biodiversity of endemic her... more Tangkahan Conservation Forest in Karo County, North Sumatera has high biodiversity of endemic herbs. Many species of the wild herbs are well known used as traditional medicine not only by local people but also by people out of the area. The methanol extract of the medicinal wild herbs in Tangkahan Conservation Forest, Karo County to relief skin diseases caused by bacteria and fungi never been studied medically. The antimicrobial activity leave extract of the medicinal herbs to pathogenic microorganisms are studied. The leaves extract of kembu-kembu (Callicarpa candicans), rintih bulung (Piper muricatum), cep-cepan (Castanopsis costata), and sereh kayu (Eugenia grandis), has antimicrobial to bacteria (Bacillus sp., Escherichia coli, Serratia marcescens, Staphylococcus aureus) and yeast (Candida albicans). Toxicity assay of these plants by brine shrimp method using Artemia salina indicates that cep-cepan dan sereh kayu have lethal concentration higher than kembu-kembu and rintih bulung.
International Journal of ChemTech Research, 2019
A study on isolation and antibacterial activity of endophytic bacteria of gambir (Uncaria gambir ... more A study on isolation and antibacterial activity of endophytic bacteria of gambir (Uncaria gambir (Hunter) Roxb.), along with its antibacterial extract activity test on clinical pathogenic bacteria has been conducted. Isolation of gambir endophytic bacteria was done in nutrient agar incubated at ambient temperature for 48 hours. Samples of gambir leaves and root was used for source of endophytic bacteria. Bacterial isolate was propagated using Mueller Hinton Agar. Bacterial culture was extracted using methanol as solvent. To test methanol extract activity of bacterial isolates, concentration of 20, 40, 60, 80, and 100% (v/v) were used in paper disc. Pathogenic bacterial isolates such as Escherichia coli, Streptococcus mutans, and Staphylococcus aureus were used for antibacterial test. Inhibition zone formed around paper disc indicated antibacterial extract activity against the bacteria. Endophytic bacterial isolates of gambirwas identified using Vitek Compact 2.0® based on biochemica...
To propagate bacterial cell for biocontrol purpose, suitable nutrient have to be determined in wh... more To propagate bacterial cell for biocontrol purpose, suitable nutrient have to be determined in which carbon and nitrogen source was often as limited factor of bacterial growth. Proper storage for biocontrol agent such as bacterial cell should also be considered in order to keep the cell viable when used. The aim of this study is to find out suitable carbon and nitrogen sources for viability and swarming of chitinolitic bacterial Bacillus sp. BK17 and Enterobacter sp. BK15. The highest population of bacterial growth (3.7x10 8 cfu/ml) was found in molases-sodium nitrate (MS) medium and the lowest population was found in crab shell-sodium nitrate (CS) growth (2.4x10 8 cfu/ml) after 25 days of incubation. The swarming activity of the isolates were varied to some extent with the highest was 51 mm in 2% agar molases-urea after 5 days of incubation. Molases-sodium nitrate (MS) medium is suitable carbon and nitrogen source for the viability of Bacillus sp. and Enterobacter sp. Meanwhile, ag...
Bakteri patogen oportunistik merupakan bakteri yang secara alami bukan berada di habitat suatu li... more Bakteri patogen oportunistik merupakan bakteri yang secara alami bukan berada di habitat suatu lingkungan tetapi masuk akibat tercemarnya lingkungan tersebut dengan limbah manusia. Keberadaan bakteri patogen oportunistik di tambak udang disebabkan karena tercemarnya air tambak oleh limbah industri maupun domestik. Penelitian ini bertujuan untuk mengetahui keberadaan bakteri patogen oportunistik pada tambak udang di Sumatera Utara (Percut, Pantai Labu, Pantai Cermin). Bakteri patogen oportunistik diisolasi dari usus udang, sedimen dan air tambak udang dengan menggunakan media selektif/diferensial. Dari hasil isolasi ditemukan adanya bakteri Escherichia coli, Salmonella sp., dan Staphylococcus sp. Jumlah tertinggi dari ketiga bakteri tersebut ditemukan pada sampel yang berasal dari daerah Percut.
Journal of Pure and Applied Microbiology, 2020
Keratinase is a proteolytic enzyme capable on degrading the hardy polymeric biomolecule or kerati... more Keratinase is a proteolytic enzyme capable on degrading the hardy polymeric biomolecule or keratin. In recent days, the utilization of keratinolytic microorganisms is seen as a promising way in recycling the keratin wastes from the avian and mammalians into valuable derived products. Previous study has reported the presence of a keratinolytic bacterium, later identified as Azotobacter chroococcum B4 obtained from dump soils. The present study investigates the enzyme characteristics of keratinase produced by this strain based on the physical appearance of final degraded product using SEM, the molecular weight of keratinase using SDS-PAGE, the effects of nutrition (C/N-source) on strain production of keratinase and the enzyme stability in metal ions solution. The molecular weight of keratinase produced by A. chroococcum B4 was about 30 kDa. Both sucrose and tryptone supplementation increase the keratinase activity by 71.7 and 97.8 U/mL after 96 h of cultivation. Metal ions, Ca 2+ , Mg 2+ , Mn 2+ , Na + , and K + are regarded as activators by increasing the relative activity of keratinase by 117, 166, 111, 113, and 112% respectively, while phenylmethylsulfonyl flouride (PMSF) is regarded as inhibitor by decreasing the relative activity down to 31%. Based on the metal ion characteristics, this strain produced a serine-protease type of keratinase which may further studied for its application in the field.
Asian Journal of Pharmaceutical and Clinical Research, 2018
Objective: This study was to analyze the karamunting leaf extract and fractions effectiveness ag... more Objective: This study was to analyze the karamunting leaf extract and fractions effectiveness against Staphylococcus aureus and Pseudomonas aeruginosa infection.Methods: Phytochemical screening is done in the extract and fractions of karamunting leaf continue with Standard agar well diffusion method for S. aureus and P. aeruginosa. Dimethyl sulfoxide was used as negative control, and standard antibiotic Kalmicetine (chloramphenicol) was used as positive control. Result: The ethyl acetate fraction showed the highest antibacterial activity against S. aureus and P. aeruginosa. Conclusion: Karamunting leaf can be used to treat the bacterial infection diseases especially infection from S. aureus and P. aeruginosa.
IOP Conference Series: Earth and Environmental Science, 2018
View the article online for updates and enhancements. Content from this work may be used under th... more View the article online for updates and enhancements. Content from this work may be used under the terms of the Creative Commons Attribution 3.0 licence. Any further distribution of this work must maintain attribution to the author(s) and the title of the work, journal citation and DOI.