Susan Marie - Academia.edu (original) (raw)
Papers by Susan Marie
Spine, 2018
Study Design. Case-control study. Objective. To identify laboratory markers for surgical site inf... more Study Design. Case-control study. Objective. To identify laboratory markers for surgical site infection (SSI) in posterior lumbar decompression surgery, which are not affected by operative factors, and to determine the diagnostic cutoffs of these markers. Summary of Background Data. Numerous laboratory markers are used for the early detection of SSI; however, these markers may be affected by operative factors. Methods. The study included 182 participants. They were divided into an SSI group (patients who developed deep SSI; n ¼ 8) and a no-SSI group (n ¼ 174). We reviewed data on the Creactive protein level and total white blood cell count and differential count before posterior lumbar decompression surgery and 1 and 4 days postoperatively. We determined which markers differed significantly between the groups and identified the markers that were not affected by operative factors (operative time, intraoperative blood loss, and number of operative segments) in the no-SSI group. We then determined the diagnostic cutoffs of these unaffected markers using receiver operating characteristic curves. Results. We identified the lymphocyte percentage at 4 days postoperatively (cutoff, <19.4%; sensitivity, 80.0%; specificity, 62.5%; area under the curve, 0.78) and lymphocyte count at 4 days postoperatively (cutoff, <1010/mL; sensitivity, 93.7%; specificity, 62.5%; area under the curve, 0.78) as reliable markers. Conclusion. Lymphocyte percentage and count at 4 days postoperatively are reliable markers for SSI after posterior lumbar decompression surgery. Lymphocyte count at 4 days postoperatively can be considered as a superior marker for screening because it has a high sensitivity and can be measured early.
Spine Surgery and Related Research, 2018
Introduction: To identify the temporal comparison of biochemical markers for early detection of s... more Introduction: To identify the temporal comparison of biochemical markers for early detection of surgical site infection (SSI) following instrumented spinal fusion that are not affected by operative factors. Methods: We reviewed data on C-reactive protein level and total white blood cell count and differential count before instrumented spinal fusion and at 1, 4, and 7 days postoperatively. The 141 patients in our sample were divided into an SSI group (patients who developed deep SSI) and a non-SSI group. We investigated the peak or nadir value day and identified those not affected by operative circumstances (operating time, intraoperative blood loss, and number of fusion segments) in the non-SSI group. If there was a significant difference between the peak or nadir value day and the next survey day, we considered the temporal comparison between these unaffected markers as an indicator of SSI and examined the usefulness of these indicators by calculating sensitivity and specificity. Furthermore, we investigated the usefulness of the combination of these markers (if even each one marker was recognized, we considered it positive). Results: Four biochemical markers of SSI were selected: neutrophil percentage at postoperative day 4 more than day 1 (sensitivity 36%, specificity 95%), neutrophil count at postoperative day 4 more than day 1 (sensitivity 46%, specificity 93%), lymphocyte percentage at postoperative day 4 less than day 1 (sensitivity 36%, specificity 90%), and lymphocyte count at postoperative day 4 less than day 1 (sensitivity 36%, specificity 90%). The combination of these markers showed sensitivity 100%, specificity 80%, respectively. Conclusions: Four markers are reliable indicators for early detection of SSI following spinal instrumented fusion because they are not affected by operative factor. The combination of each indicator had both high sensitivity and specificity. Therefore, it is reliable and much useful for early detection of SSI.
日本脊椎脊髄病学会雑誌 the Journal of the Japan Spine Research Society, Dec 1, 2007
Journal of Spine Research Official Journal of the Japanese Society For Spine Surgery and Related Research, Sep 1, 2014
Key Engineering Materials, 2005
Marrow mesenchymal cells contain stem cells and can regenerate tissue. We previously reported the... more Marrow mesenchymal cells contain stem cells and can regenerate tissue. We previously reported the clinical application of autologous cultured bone to regeneration therapy. However there is room for improvement in the culture methods; and here, we examined the optimal frequency of medium changing. Marrow cells were collected from the femur of a Fisher 7 week-old male rat. At 2 weeks after primary culture in a standard medium (MEM containing 15% bovine fetal serum), the cultures were trypsinized to prepare a cell suspension and divided into two groups, with or without addition of dexamethasone (Dex) to the osteogenic medium. To investigate optimal frequency, we further divided into 5 sub-groups; no changing (M0), 1 time/week (M1), 2/week (M2), 3/week (M3), and everyday (M7). After 2 weeks of subculture, the tissue was harvested and then ALP activity and calcium and DNA contents measured. In both of the Dex groups, there was significantly high ALP activity in the higher frequency group...
Stem Cell Studies, 2011
Spinal fusion is performed using bone harvested from the ilium. However, graft harvest is associa... more Spinal fusion is performed using bone harvested from the ilium. However, graft harvest is associated with frequent complications and pain. If a tissue engineered bone (TEB) was available, spinal fusion could be performed without damaging normal tissues. In 8 patients, 10-20 mL of marrow fluid was collected from the iliac crest to fabricate. After primary culture in the standard medium, marrow mesenchymal cells were combined with porous tricalcium phosphate block and were cultured in osteogenic medium containing dexamethasone, b-glycerophosphate, vitamin C phosphate, and estriol. After 3 weeks of subculture, spinal fusion was performed using TEB. Nine patients who had undergone spinal fusion using iliac autografts served as the controls (AG group). In all patients, significant improvement in JOA score was seen in both TEB and AG groups. The radiographic fusion rate was 87.5% (7/8) in TEB group and 77.8% (2/9) in AG group at 6 months after surgery. The mean operating time in TEB group was shorter than in the AG group. Compared with the AG group, the patients receiving TEB graft had significantly less total blood loss. In the AG group, all of the patients complained of graft site pain for 2 to 4 weeks after the operation. Two patients (22.2%) still had graft site pain at 6 months postoperatively. Bone regeneration therapy using the TEB graft introduced in this report makes it possible to perform spinal fusion as is done using autogenous bone grafts, but with the minimally invasive procedure of bone marrow aspiration.
This study tested hypotheses about the dynamics and function of male/female affiliative relations... more This study tested hypotheses about the dynamics and function of male/female affiliative relationships (friendships) in captive chimpanzees by collecting data relevant to (1) the circumstances surrounding the formation of friendships; (2) the types of anestrous/estrous interaction between males and females once a friendship has formed; (3) the basis for particular partner preferences; and (4) the potential benefits of friendships. The data were collected over a 15-month period on a semi-free ranging colony of eleven chimpanzees at the Detroit Zoo in Michigan. This study was the first to investigate the formation and special characteristics of friendships in chimpanzees. Friends were defined on the basis of time spent in spatial proximity and grooming. Based on these criteria, three male/female dyads clearly formed friendships. The circumstances surrounding friendship formation and the special characteristics of friendly relationships, however, were less clear. The data indicated that (1) the frequency and degree of heterosexual interaction during the female's first three estrous cycles only weakly predicted later friendships; (2) among Friends, one individual was responsible for most grooming interaction, but the sex responsible for grooming was inconsistent among those dyads defined as Friends; (3) among Friends, the same individual was responsible for both grooming and maintaining proximity; and (4) females avoided proximity with their male Friends when in estrus. The benefits of forming friendships were difficult to determine. Some evidence suggested that one male received the benefit of sexual access to his Friend while she was in estrus. For both males and females, support during aggressive interaction with other group members was not a benefit of friendship formation. Female partner preference was probably based on male dominance rank or male temperament; male partner preference was probably based on a combination of female age/dominance rank, and potential agonistic support. Although the results are not conclusive, this study provides a detailed description of the introduction of unfamiliar males and females and the subsequent development of male/female affiliative relationships among captive chimpanzees, which will help to further our understanding of both chimpanzee social relationships and the evolution of hominid pair bonding.Ph.D.AnthropologyUniversity of Michigan, Horace H. Rackham School of Graduate Studieshttp://deepblue.lib.umich.edu/bitstream/2027.42/103271/1/9308396.pdfDescription of 9308396.pdf : Restricted to UM users only
Spine, 2018
Study Design. Case-control study. Objective. To identify laboratory markers for surgical site inf... more Study Design. Case-control study. Objective. To identify laboratory markers for surgical site infection (SSI) in posterior lumbar decompression surgery, which are not affected by operative factors, and to determine the diagnostic cutoffs of these markers. Summary of Background Data. Numerous laboratory markers are used for the early detection of SSI; however, these markers may be affected by operative factors. Methods. The study included 182 participants. They were divided into an SSI group (patients who developed deep SSI; n ¼ 8) and a no-SSI group (n ¼ 174). We reviewed data on the Creactive protein level and total white blood cell count and differential count before posterior lumbar decompression surgery and 1 and 4 days postoperatively. We determined which markers differed significantly between the groups and identified the markers that were not affected by operative factors (operative time, intraoperative blood loss, and number of operative segments) in the no-SSI group. We then determined the diagnostic cutoffs of these unaffected markers using receiver operating characteristic curves. Results. We identified the lymphocyte percentage at 4 days postoperatively (cutoff, <19.4%; sensitivity, 80.0%; specificity, 62.5%; area under the curve, 0.78) and lymphocyte count at 4 days postoperatively (cutoff, <1010/mL; sensitivity, 93.7%; specificity, 62.5%; area under the curve, 0.78) as reliable markers. Conclusion. Lymphocyte percentage and count at 4 days postoperatively are reliable markers for SSI after posterior lumbar decompression surgery. Lymphocyte count at 4 days postoperatively can be considered as a superior marker for screening because it has a high sensitivity and can be measured early.
Spine Surgery and Related Research, 2018
Introduction: To identify the temporal comparison of biochemical markers for early detection of s... more Introduction: To identify the temporal comparison of biochemical markers for early detection of surgical site infection (SSI) following instrumented spinal fusion that are not affected by operative factors. Methods: We reviewed data on C-reactive protein level and total white blood cell count and differential count before instrumented spinal fusion and at 1, 4, and 7 days postoperatively. The 141 patients in our sample were divided into an SSI group (patients who developed deep SSI) and a non-SSI group. We investigated the peak or nadir value day and identified those not affected by operative circumstances (operating time, intraoperative blood loss, and number of fusion segments) in the non-SSI group. If there was a significant difference between the peak or nadir value day and the next survey day, we considered the temporal comparison between these unaffected markers as an indicator of SSI and examined the usefulness of these indicators by calculating sensitivity and specificity. Furthermore, we investigated the usefulness of the combination of these markers (if even each one marker was recognized, we considered it positive). Results: Four biochemical markers of SSI were selected: neutrophil percentage at postoperative day 4 more than day 1 (sensitivity 36%, specificity 95%), neutrophil count at postoperative day 4 more than day 1 (sensitivity 46%, specificity 93%), lymphocyte percentage at postoperative day 4 less than day 1 (sensitivity 36%, specificity 90%), and lymphocyte count at postoperative day 4 less than day 1 (sensitivity 36%, specificity 90%). The combination of these markers showed sensitivity 100%, specificity 80%, respectively. Conclusions: Four markers are reliable indicators for early detection of SSI following spinal instrumented fusion because they are not affected by operative factor. The combination of each indicator had both high sensitivity and specificity. Therefore, it is reliable and much useful for early detection of SSI.
日本脊椎脊髄病学会雑誌 the Journal of the Japan Spine Research Society, Dec 1, 2007
Journal of Spine Research Official Journal of the Japanese Society For Spine Surgery and Related Research, Sep 1, 2014
Key Engineering Materials, 2005
Marrow mesenchymal cells contain stem cells and can regenerate tissue. We previously reported the... more Marrow mesenchymal cells contain stem cells and can regenerate tissue. We previously reported the clinical application of autologous cultured bone to regeneration therapy. However there is room for improvement in the culture methods; and here, we examined the optimal frequency of medium changing. Marrow cells were collected from the femur of a Fisher 7 week-old male rat. At 2 weeks after primary culture in a standard medium (MEM containing 15% bovine fetal serum), the cultures were trypsinized to prepare a cell suspension and divided into two groups, with or without addition of dexamethasone (Dex) to the osteogenic medium. To investigate optimal frequency, we further divided into 5 sub-groups; no changing (M0), 1 time/week (M1), 2/week (M2), 3/week (M3), and everyday (M7). After 2 weeks of subculture, the tissue was harvested and then ALP activity and calcium and DNA contents measured. In both of the Dex groups, there was significantly high ALP activity in the higher frequency group...
Stem Cell Studies, 2011
Spinal fusion is performed using bone harvested from the ilium. However, graft harvest is associa... more Spinal fusion is performed using bone harvested from the ilium. However, graft harvest is associated with frequent complications and pain. If a tissue engineered bone (TEB) was available, spinal fusion could be performed without damaging normal tissues. In 8 patients, 10-20 mL of marrow fluid was collected from the iliac crest to fabricate. After primary culture in the standard medium, marrow mesenchymal cells were combined with porous tricalcium phosphate block and were cultured in osteogenic medium containing dexamethasone, b-glycerophosphate, vitamin C phosphate, and estriol. After 3 weeks of subculture, spinal fusion was performed using TEB. Nine patients who had undergone spinal fusion using iliac autografts served as the controls (AG group). In all patients, significant improvement in JOA score was seen in both TEB and AG groups. The radiographic fusion rate was 87.5% (7/8) in TEB group and 77.8% (2/9) in AG group at 6 months after surgery. The mean operating time in TEB group was shorter than in the AG group. Compared with the AG group, the patients receiving TEB graft had significantly less total blood loss. In the AG group, all of the patients complained of graft site pain for 2 to 4 weeks after the operation. Two patients (22.2%) still had graft site pain at 6 months postoperatively. Bone regeneration therapy using the TEB graft introduced in this report makes it possible to perform spinal fusion as is done using autogenous bone grafts, but with the minimally invasive procedure of bone marrow aspiration.
This study tested hypotheses about the dynamics and function of male/female affiliative relations... more This study tested hypotheses about the dynamics and function of male/female affiliative relationships (friendships) in captive chimpanzees by collecting data relevant to (1) the circumstances surrounding the formation of friendships; (2) the types of anestrous/estrous interaction between males and females once a friendship has formed; (3) the basis for particular partner preferences; and (4) the potential benefits of friendships. The data were collected over a 15-month period on a semi-free ranging colony of eleven chimpanzees at the Detroit Zoo in Michigan. This study was the first to investigate the formation and special characteristics of friendships in chimpanzees. Friends were defined on the basis of time spent in spatial proximity and grooming. Based on these criteria, three male/female dyads clearly formed friendships. The circumstances surrounding friendship formation and the special characteristics of friendly relationships, however, were less clear. The data indicated that (1) the frequency and degree of heterosexual interaction during the female's first three estrous cycles only weakly predicted later friendships; (2) among Friends, one individual was responsible for most grooming interaction, but the sex responsible for grooming was inconsistent among those dyads defined as Friends; (3) among Friends, the same individual was responsible for both grooming and maintaining proximity; and (4) females avoided proximity with their male Friends when in estrus. The benefits of forming friendships were difficult to determine. Some evidence suggested that one male received the benefit of sexual access to his Friend while she was in estrus. For both males and females, support during aggressive interaction with other group members was not a benefit of friendship formation. Female partner preference was probably based on male dominance rank or male temperament; male partner preference was probably based on a combination of female age/dominance rank, and potential agonistic support. Although the results are not conclusive, this study provides a detailed description of the introduction of unfamiliar males and females and the subsequent development of male/female affiliative relationships among captive chimpanzees, which will help to further our understanding of both chimpanzee social relationships and the evolution of hominid pair bonding.Ph.D.AnthropologyUniversity of Michigan, Horace H. Rackham School of Graduate Studieshttp://deepblue.lib.umich.edu/bitstream/2027.42/103271/1/9308396.pdfDescription of 9308396.pdf : Restricted to UM users only