Susana Zeni - Academia.edu (original) (raw)

Papers by Susana Zeni

El pico de masa ósea (PMO) se alcanza entre los 20 y 35 años, pero la aposición ósea continúa has... more El pico de masa ósea (PMO) se alcanza entre los 20 y 35 años, pero la aposición ósea continúa hasta alcanzar el pico de fortaleza ósea (PFO). Se crea así una ventana entre ambos picos que podría ser evaluada mediante marcadores bioquímicos de recambio óseo, ya que durante dicho período la densidad mineral permanece constante. El objetivo fue determinar el final de la aposición ósea mediante marcadores bioquímicos óseos. Se evaluaron por décadas entre 20 y 49 años de edad 139 sujetos sanos de ambos sexos (69 hombres y 70 mujeres), determinando fosfatasa alcalina ósea (FAO), osteocalcina (OC), propéptido amino terminal del colágeno tipo 1 (P1NP) y telopéptido C-terminal del colágeno tipo 1 (CTX). Los marcadores correlacionan negativamente con la edad (OC: r= -0,3; p<0,01; P1NP: r= -0,4; p< 0,01 y CTX: r= -0,4; p< 0,01), exceptuando FAO. En hombres de 20-29 años, P1NP y el CTX fueron significativamente mayores vs. 30-39 años (p<0,05 y p<0,001, respectivamente), y entre 3...

Austin Publishing Group, Oct 10, 2019

Background: Bone remodeling, insulin levels and fat mass interrelationship in glucose homeostasis... more Background: Bone remodeling, insulin levels and fat mass interrelationship in glucose homeostasis control was evaluated in Weaning Normal (W) and Obese (O) rats fed High (H), Normal (N) or Low (L) Ca intakes. Methods: Glucose, Cholesterol (Chol), HDL-Chol, Triglyceride (TGL), Ca, P, insulin, Osteocalcin (OCN) and collagen C-telopeptide (CTX), body composition, BMD, BMC, body Ca and P content, perigonadal plus retroperitoneal fat (PG+RP) and liver weight were determined and HOMA-IR calculated. Results: WHCa reached the highest body fat, PG+RP and the highest CTX levels (p<0.05); WNCa had the lowest liver weight. WLCa reached the lowest body protein content (p<0.05) and the highest glucose, insulin and HOMA-IR (p<0.05). WLCa and WHCa had similar Chol levels but higher than WNCa; TGL increased and OCN decreased as dietary Ca content increased (p<0.05). OLCa presented the highest body fat, Chol and OCN levels but the lowest HDL-Chol levels (p<0.05); ONCa had the highest body protein percentage (p<0.05). OHCa had the lowest CTX levels (p<0.05). PG+RP, liver weight, glucose, insulin and HOMA-IR decreased as dietary Ca content increased (p<0.05). O groups reached higher adipose PG+RP fat, liver weight, glucose, insulin, Chol, TGL and HOMA-IR and lower OCN, CTX and body protein content than their matched-W groups (p<0.05). Conclusion: The relative amount of dietary Ca to P may regulate energy metabolism and bone turnover, insulin and body fat interplay in glucose homeostasis control. However, the mechanisms differ in physiological conditions or in the presence of metabolic abnormalities of energy dysregulation such as obesity and T2-diabetes.

Asociación Argentina de Osteología y Metabolismo Mineral, Oct 1, 2020

Calcified Tissue International

European Journal of Nutrition, 2014

Aim Increasing calcium intake is the most effective strategy for avoiding Ca deficit. However, if... more Aim Increasing calcium intake is the most effective strategy for avoiding Ca deficit. However, if intake remains inadequate, improving Ca absorption becomes an important tool to optimize Ca homeostasis and bone health. Purpose The effect of a mixture of GOS/FOS Ò 9:1 added to a normal-or low-Ca diets on Ca absorption and bone mineralization, density and structure was investigated, in a model of growing rats. Several colonic parameters to help support the findings were also evaluated. Results Weanling Wistar rats received one of the four experimental AIN-93G diets: C5: 0.5 % Ca; C3: 0.3 % Ca; P5: 0.5 % Ca ? 5.3 % GOS/FOS Ò ; P3: 0.3 % Ca ? 5.3 % GOS/FOS Ò until 50 days (T = 50). At T = 50, lactobacillus and cecum weights were higher, whereas cecum pH was lower in P5 and P3 versus C5 and C3 (p \ 0.001). At T = 50, fecal Ca, Mg and P were lower and their absorptions (mg/dL) were higher in P5 and P3 versus C5 and C3, respectively (p \ 0.05). Ca, Mg and P absorption % was higher in P5 and P3 versus C5 and C3 (p \ 0.001). Femur Ca and P content, bone mineral content, trabecular bone mineral density, tibia length, bone volume, osteoblast surface, stiffness and elastic modulus were higher in P5 and P3 versus C5 and C3 (p \ 0.05). Despite the lower Ca content, P3 group reached similar values than C5 in all these latter parameters. Conclusions Supplementing diets with the GOS/FOS Ò mixture increased bone mineralization, density and structure due to an increase in Ca, P and Mg absorptions. Thus, this prebiotic mixture may help to improve bone development in a period of high calcium requirements.

European Journal of Nutrition, 2013

Purpose A low calcium intake (LCaI) may predispose to obesity, and excessive fat mass may be detr... more Purpose A low calcium intake (LCaI) may predispose to obesity, and excessive fat mass may be detrimental to bone. The impact of Ca inadequacy would be greater in subjects predisposed to obesity. LCaI effect on obesity development during the rapid growth period was compared in two strains of rats: spontaneously obese IIMb/b (O) and Wistar (W). Pregnant rats were fed 0.5 % (N) or 0.2 % (L) of Ca (OLCa, ONCa, WLCa and WNCa). Male pups were fed the maternal diet until day 60. Methods Body composition, lipid profile, glucose homeostasis, 25 hydroxyvitamin D, Ca-phosphorus, and bone metabolism were evaluated. Results BW and body fat were higher, whereas body protein was lower in OLCa versus ONCa (p \ 0.05). OLCa presented the highest body fat, glucose, non-HDL and total cholesterol, TGL, insulin levels, and HOMA-IR, liver weight, and adipose perigonadal plus retroperitoneal pads (p \ 0.05). WLCa did not exhibit an increase BW and only showed a slight change in body composition with minor biochemical alterations compared to WNCa (p \ 0.05). Osteocalcin, CTX, and proximal tibia and lumbar spine BMDs were lower in O than in W rats fed the same Ca diet (p \ 0.05). Body ash and Ca content, and total skeleton BMC/BW were lower in OLCa and WLCa versus their corresponding NCa groups (p \ 0.05). Conclusion The negative effect of a low Ca diet on fat mass accumulation and lipid profile may be more evident in rats predisposed to obesity. Nevertheless, low CaI interferes with the normal glucose homeostasis leading to an increase in insulin resistance. Low CaI during early growth may be an obesogenic factor that may persist into adult life and may account for the development of obesity and some of its co-morbidities.

European Journal of Nutrition, 2012

Calcified Tissue International, 2003

The purpose of this study was to quantify in vivo the impact of different dietary Ca contents on ... more The purpose of this study was to quantify in vivo the impact of different dietary Ca contents on the maternal total skeleton and skeletal sub-areas in adult rats during pregnancy and lactation, using DXA. Twenty-four female Wistar rats (approximately 5 months old) were mated and divided into three groups (n = 8) and fed one of the following diets, varying only in Ca content (LCD: 0.14%, NCD: 0.6% or HCD: 1.2%). Pups were adjusted to 8-9 per dam. Maternal ionic calcium and in vivo bone mineral density (BMD) were measured at the beginning, after delivery and after weaning. Regardless of the diet, ionized calcium decreased from onset to weaning ( P &amp;amp;amp;amp;amp;amp;amp;lt; 0.05). At weaning, bone mass decreased 7.3% in NCD, 15% in LCD and 10.5% in HCD from initial values. Total skeleton, whole and proximal tibia and spine BMDs only decreased at delivery in the LCD group ( P &amp;amp;amp;amp;amp;amp;amp;lt; 0.05) but, irrespective of the diet, at weaning, they were lower compared to delivery and initial values ( P &amp;amp;amp;amp;amp;amp;amp;lt; 0.05). LCD group presented the lowest BMD in the proximal tibia and spine regions ( P &amp;amp;amp;amp;amp;amp;amp;lt; 0.05). At birth, pups did not present differences, however, at weaning, LCD pups reached the lowest body weight ( P &amp;amp;amp;amp;amp;amp;amp;lt; 0.05), NCD presented the highest body Ca content ( P &amp;amp;amp;amp;amp;amp;amp;lt; 0.05) and there were no differences between LCD and HCD. This in vivo study showed that regardless of the dietary calcium content, the maternal skeleton is slightly affected by pregnancy but severely affected by lactation. However, the degree of such response appears to depend not only on dietary Ca content but also on dietary Ca/P molar ratio.

Bone, 2003

This longitudinal study evaluated bone turnover and the interrelationship between changes in bone... more This longitudinal study evaluated bone turnover and the interrelationship between changes in bone biomarkers and habitual dietary calcium intake during pregnancy in a group of women ranging widely with regard to dietary calcium intake. Thirty-nine healthy pregnant and 30 nonpregnant women were studied. Calcium, phosphorus, 1alpha,25-dihydroxyvitamin D (1,25diHOD), bone alkaline phosphatase (bALP), carboxyterminal propeptides of type I procollagen (PICP) and carboxyterminal telopeptides of type I collagen (betaCTX and ICTP) were measured in serum and calcium, and creatinine and aminoterminal telopeptide (NTX) were determined in urine. Serum calcium and phosphorus did not change but the urinary Ca/Creat ratio and 1,25diHOD increased throughout pregnancy (P &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.001 and P &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.0001, respectively). Serum b-ALP and PICP increased during the last two trimesters (P &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.0001 and P…

Bone, 2016

Rabbit mesenchymal stem cells (rMSC) were seeded on different ratios of Hydroxyapatite/Collagen t... more Rabbit mesenchymal stem cells (rMSC) were seeded on different ratios of Hydroxyapatite/Collagen type I (HA/CoI) coating amounts in an attempt to mimic a remodeling bone environment in which they attach and differentiate. HA nanomaterial was composed of nanoparticles with similar physicochemical characteristics of mineral bone matrix. Recently it was shown that the elevation of α-smooth muscle actin (α-SMA) expression is associated with MSC's osteogenic potential. Also, actin cytoskeleton organization is sensitive to material's topography. Therefore, during this study MSC's adhesion and spreading on HA/CoI coatings, with the same inorganicorganic matrix proportion of the bone tissue, was evaluated by α-SMA immunostaining, number of cells attached and MTT assay. Images obtained by 2-photon laser confocal microscopy showed that attached cells had a typical MSC morphology, with a spread polygonal shape and cytoplasmic extensions; also homogeneous cell distribution was found on biomimetic coatings using HA amounts from 15 to 62 μg/cm 2 and 31 μg/cm 2 of CoI. Semi-quantitative images analysis showed that α-SMA content was the same as the control and MTT assay demonstrated that mitochondrial metabolic activity was not affected (p N 0.10). On the other hand, cells seeded on a hundred times larger HA coating amount showed less spreading and rounded cells, also adhesion and metabolic activity were inhibited (p ˂ 0.01). The obtained results define a maximal MSC cytocompatible HA coating amount. On the whole, results obtained showed that no differences were found on cells viability, cytoskeleton integrity and MSC phenotype on the different biomimetic coating tested.

Los hallazgos osteológicos se intensificaron en los últimos años. Se demostró que el esqueleto se... more Los hallazgos osteológicos se intensificaron en los últimos años. Se demostró que el esqueleto se comporta, además de sus funciones clásicas, como un órgano de secreción endocrina que sintetiza al menos dos hormonas: el factor de crecimiento de fibroblastos 23 (FGF-23) y la osteocalcina (Ocn).La Ocn es un péptido pequeño que contiene 3 residuos de ácido glutámico. Estos residuos se carboxilan postraduccionalmente, quedando retenida en la matriz ósea. La forma decarboxilada en el primer residuo de ácido glutámico (GluOcn) fue reportada por poseer efectos biológicos; la resorción ósea es el mecanismo clave para su bioactivación.La presente revisión se centra en los conocimientos actuales sobre la función hormonal de la Ocn. A la fecha se reporta que la Ocn regularía el metabolismo energético aumentando la proliferación de células β pancreáticas, y la secreción de insulina y de adiponectina. Sobre el músculo esquelético actuaría favoreciendo la absorción y el catabolismo de nutrientes....

La prevalencia de dos desórdenes crónicos como la osteoporosis y la obesidad se encuentra en aume... more La prevalencia de dos desórdenes crónicos como la osteoporosis y la obesidad se encuentra en aumento. La fisiopatología de ambas alteraciones metabólicas es multifactorial e incluye factores genéticos, factores ambientales y hormonales. Diversos estudios clínicos y experimentales demuestran la existencia de una interacción entre el tejido adiposo y el esqueleto, similar a la que ocurre entre las patologías mencionadas. Si bien se pensaba que la obesidad protegía al hueso, actualmente se postula que un exceso de tejido graso, fundamentalmente grasa abdominal, sería un factor de riesgo para el desarrollo de osteoporosis y fracturas por fragilidad. Diversos factores secretados por el tejido graso incrementados por efecto de la obesidad jugarían un rol clave en la salud ósea. Las adipoquinas, citokinas y ácidos grasos libres regulan el remodelamiento óseo, disminuyendo la formación e incrementando la resorción, al mismo tiempo que inducen estrés oxidativo e hiperglucemia, que exacerba e...

El Síndrome Metabólico (SM) es una patología heterogénea que en rangos generales se podría defini... more El Síndrome Metabólico (SM) es una patología heterogénea que en rangos generales se podría definir como la combinación de factores de riesgo asociados a la enfermedad cardiovascular (ECV) y diabetes, caracterizados por insulino-resistencia, hipertensión, elevada presión arterial, elevados niveles de triglicéridos (TGL) y obesidad abdominal.La hipovitaminosis D induciría perturbaciones metabólicas (hipertensión, aterogenicidad, incremento de ECV, de insulino-resistencia, de diabetes tipo II y de obesidad central) que favorecen el desarrollo de SM. El estado nutricional respecto de la vitamina D se evalúa por los niveles de 25hidroxivitamina D (25OHD), principal metabolito circulante de dicha vitamina. Diversos estudios han sugerido la existencia de una relación inversa entre los niveles de 25OHD y el desarrollo de SM. La adiposidad central, la resistencia a la insulina y aterogenicidad son importantes factores de riesgo para el desarrollo de SM. Todos ellos se asocian a la hipovitami...

Las mitocondrias generan especies reactivas de oxígeno (ERO) que cumplen con una multiplicidad de... more Las mitocondrias generan especies reactivas de oxígeno (ERO) que cumplen con una multiplicidad de procesos celulares; cuando se producen en exceso son responsables del estrés oxidativo y de múltiples procesos patológicos, incluyendo osteoporosis. Los factores de transcripción FoxO 1, 3 y 4 actúan como moléculas sensoras de ERO convirtiendo la señal de estrés oxidativo en la inducción de mecanismos de protección o señales apoptóticas. La insulina y los factores de crecimiento insulínicos (IGFs) regulan negativamente a FoxOs en mamíferos. Las ERO están involucradas en el remodelamiento óseo a través del efecto que ejercen sobre osteoblastos y osteoclastos. Los FoxOs controlan la acción de ERO sobre la osteoblastogénesis y la osteoclastogénesis. Con la edad, el aumento del estrés oxidativo acelera la adipogénesis a expensas de la osteoblastogénesis, al mismo tiempo que aumenta la oxidación de ácidos grasos generando compuestos pro-oxidantes que incrementan el estrés oxidativo. Asimismo...

Austin Journal of Dentistry, 2017

Aim: Oral health and nutrition have a synergistic bidirectional relationship. Diet plays an impor... more Aim: Oral health and nutrition have a synergistic bidirectional relationship. Diet plays an important role in dental caries process, one of the main factors associated with high risk of tooth loss in adults. Conversely, tooth loss, pain and discomfort limit food choices which negatively affect nutritional status. The objective was to evaluate association between oral health and calcium (Ca) and vitamin D nutritional status, several dietary and lifestyle habits in young men. Materials and Methods: A total of 241 men (29.4±2.1 years) were studied. Ca intake (CaI) and protein intakes were recorded, and 25hydroxyvitamin D (25OHD) was determined. Dental status and caries risk were assessed by Decayed (D), Missing (M) and Filled (F) Teeth, (DMFT) index, Löe Silness Plaque Index (PI) and sugar intake (SI). Results: Deficient CaI was observed in 48% of men; 71% had 25OHD <30 ng/mL and 65% consumed soft drinks daily. M/T was 2.9%; D/T was 16.2% and F/T was 18.7%; 41% were missing at least one tooth. DMFT score was 11.4±0.9, PI and SI were 1.9±0.2 and 5.3±0.2, respectively. Adjusted-CaI by risk factors was associated with higher caries activity (p<0.01) and missing teeth (p<0.013); 100% presented gingivitis. DMFT of the one third of men with the highest caries scores reached 17.4±0.5; these men had significantly lower CaI (p<0.023) and higher daily consumption of soft drinks and PI values (p<0.05). Conclusion: CaI and vitamin D inadequacies, together with certain dietary and lifestyle habits observed in the studied men would seemingly be responsible, at least in part, for their high cariogenic activity.

Journal of Investigative Medicine, 2004