Susanne Kruse - Academia.edu (original) (raw)
Related Authors
UFMG - The Federal University of Minas Gerais
Uploads
Papers by Susanne Kruse
International Immunology, 1999
IL-4 plays a major role in IgE production. Its signal is conferred to effector cells through bind... more IL-4 plays a major role in IgE production. Its signal is conferred to effector cells through binding to the α chain of the membrane-bound human IL-4 receptor (huIL-4Rα). Here we present the genomic structure and organization of huIL-4Rα. The promotor region shows binding sites for several transcription factors involved in inflammatory processes. HuIL-4Rα has been shown to be organized differently to that of mouse IL-4Rα. A soluble form of huIL-4Rα is produced by alternative splicing of the huIL-4Rα gene (shuIL-4Rα/splice). Expression of the corresponding mRNA coding for the extracellular part of the receptor and an additional three amino acids is also shown. A second form of huIL-4Rα, i.e. shuIL-4Rα/prot, is produced by limited proteolysis of the receptor (shedding) and is already known. These results reveal a complex pattern for the regulation of the IL-4 pathway at the receptor level. The patterns of expression of all three receptor proteins as well as their individual meaning in the context of inflammation still have to be elucidated.
Journal of Investigative Dermatology, 2002
Patients with atopic dermatitis display substantial immunologic abnormalities, among which elevat... more Patients with atopic dermatitis display substantial immunologic abnormalities, among which elevated total IgE is considered as a hallmark; however, a subgroup of atopic dermatitis patients exhibits normal IgE levels, but mechanisms contributing to the so-called``intrinsic'' or``nonallergic'' form of atopic dermatitis are obscure. In order to unravel similarities and differences of both atopic dermatitis subtypes, the phenotype of monocytes, total serum IgE levels, and serum levels of cytokines regulating the IgE production from nonatopic individuals and patients with allergic rhinitis, and extrinsic and intrinsic atopic dermatitis were measured. Concomitantly, genomic DNA probes of all subjects were analyzed for single nucleotide polymorphisms of candidate genes of structures involved in the regulation of the IgE synthesis, such as interleukin-4 and the interleukin-4R/interleukin-13R. Our data show that the surface expression of the high-and low-af®nity receptor for IgE (FceRI and FceRII/CD23) and the interleukin-4Ra chain were signi®cantly elevated in monocytes from patients with extrinsic atopic dermatitis. Furthermore, serum levels of interleukin-13 were signi®cantly increased in patients with intrinsic atopic dermatitis. In addition, the frequency of the interleukin-4Ra polymorphism C3223T and the interleukin-4 polymorphism C590T tended to be higher in extrinsic atopic dermatitis than in intrinsic atopic dermatitis. Altogether our ®ndings indicate that intrinsic atopic dermatitis patients exhibit phenotypic and immunologic features, which differ from those of patients with extrinsic atopic dermatitis or other atopic disorders. Key words: atopic dermatitis/FceRI/FceRII/interleukin-13/interleukin-4/monocyte/single nucleotide polymorphism/soluble form of interleukin-4R. J Invest Dermatol 119: 870±875, 2002
International Immunology, 1999
IL-4 plays a major role in IgE production. Its signal is conferred to effector cells through bind... more IL-4 plays a major role in IgE production. Its signal is conferred to effector cells through binding to the α chain of the membrane-bound human IL-4 receptor (huIL-4Rα). Here we present the genomic structure and organization of huIL-4Rα. The promotor region shows binding sites for several transcription factors involved in inflammatory processes. HuIL-4Rα has been shown to be organized differently to that of mouse IL-4Rα. A soluble form of huIL-4Rα is produced by alternative splicing of the huIL-4Rα gene (shuIL-4Rα/splice). Expression of the corresponding mRNA coding for the extracellular part of the receptor and an additional three amino acids is also shown. A second form of huIL-4Rα, i.e. shuIL-4Rα/prot, is produced by limited proteolysis of the receptor (shedding) and is already known. These results reveal a complex pattern for the regulation of the IL-4 pathway at the receptor level. The patterns of expression of all three receptor proteins as well as their individual meaning in the context of inflammation still have to be elucidated.
Journal of Investigative Dermatology, 2002
Patients with atopic dermatitis display substantial immunologic abnormalities, among which elevat... more Patients with atopic dermatitis display substantial immunologic abnormalities, among which elevated total IgE is considered as a hallmark; however, a subgroup of atopic dermatitis patients exhibits normal IgE levels, but mechanisms contributing to the so-called``intrinsic'' or``nonallergic'' form of atopic dermatitis are obscure. In order to unravel similarities and differences of both atopic dermatitis subtypes, the phenotype of monocytes, total serum IgE levels, and serum levels of cytokines regulating the IgE production from nonatopic individuals and patients with allergic rhinitis, and extrinsic and intrinsic atopic dermatitis were measured. Concomitantly, genomic DNA probes of all subjects were analyzed for single nucleotide polymorphisms of candidate genes of structures involved in the regulation of the IgE synthesis, such as interleukin-4 and the interleukin-4R/interleukin-13R. Our data show that the surface expression of the high-and low-af®nity receptor for IgE (FceRI and FceRII/CD23) and the interleukin-4Ra chain were signi®cantly elevated in monocytes from patients with extrinsic atopic dermatitis. Furthermore, serum levels of interleukin-13 were signi®cantly increased in patients with intrinsic atopic dermatitis. In addition, the frequency of the interleukin-4Ra polymorphism C3223T and the interleukin-4 polymorphism C590T tended to be higher in extrinsic atopic dermatitis than in intrinsic atopic dermatitis. Altogether our ®ndings indicate that intrinsic atopic dermatitis patients exhibit phenotypic and immunologic features, which differ from those of patients with extrinsic atopic dermatitis or other atopic disorders. Key words: atopic dermatitis/FceRI/FceRII/interleukin-13/interleukin-4/monocyte/single nucleotide polymorphism/soluble form of interleukin-4R. J Invest Dermatol 119: 870±875, 2002