Sushen Pradhan - Academia.edu (original) (raw)

Papers by Sushen Pradhan

The present investigation was carried out for the determination of genetic conformity of micropro... more The present investigation was carried out for the determination of genetic conformity of micropropagated plants of Zingiber officinale cv Majhauley using Random amplified polymorphic DNA (RAPD) technique.Total fifteen different arbitrary decamers were used as primers to amplify DNA from in vitro plant material to assess the genetic fidelity of 18 months old in vitro cultured plantlets. Total 38 amplified reproducible bands were produced from 10 primers with an average of 3.8 per RAPD primer, amplified bands were ranging from 150-950 base pair, the highest seven number of bands found in primer CLT53 (50-875 bp) and lowest one band in CLT 112 (575 bp) and CLT 192 (650 bp) primers. All the primers were found to be monomorphic and no genetic variation was detected within the micropropagated plants. Present protocol may be used for testing of ginger propagules to ensure mass production of disease free planting materials.

3 biotech, Aug 9, 2014

Dendrocalamus strictus popularly known as 'Male bamboo' is a multipurpose bamboo which is extensi... more Dendrocalamus strictus popularly known as 'Male bamboo' is a multipurpose bamboo which is extensively utilized in pharmaceutical, paper, agricultural and other industrial implements. In this study, in vitro regeneration of D. strictus through nodal culture has been attempted. Murashige and Skoog's medium supplemented with 4 mg/l BAP was found to be most effective in shoot regeneration with 3.68 ± 0.37 shoots per explant. The effect of Kn was found to be moderate. These hormones also had considerable effect on the shoot length. The highest shoot length after 6 weeks (3.11 ± 0.41 cm) was noted with 5 mg/l BAP followed by 3.07 ± 0.28 cm with 5 mg/l Kn, while decrease in the shoot length was noted with other treatments. The effect of IBA and NAA individually or in combination at different concentrations on rooting was evaluated. The highest number of root (1.36 ± 0.04) was regenerated on full-strength MS medium supplemented with 3 mg/l NAA, while maximum length of 1.64 ± 0.03 cm of roots was recorded with combination of 1 mg/l IBA and 3 mg/l NAA. Tissue-cultured plants thus obtained were successfully transferred to the soil. The clonal fidelity among the in vitro-regenerated plantlets was assessed by RAPD and ISSR markers. The ten RAPD decamers produced 58 amplicons, while nine ISSR primers generated a total of 66 bands. All the bands generated were monomorphic. These results confirmed the clonal fidelity of the tissue culture-raised D. strictus plantlets and corroborated the fact that nodal culture is perhaps the safest mode for multiplication of true to type plants.

Research Journal of Pharmacognosy and Phytochemistry, Dec 1, 2014

Vaccinium sikkimense under the family of Ericaceae is a low bush blueberry commonly found in Nort... more Vaccinium sikkimense under the family of Ericaceae is a low bush blueberry commonly found in Northern area of Sikkim Himalayas. The berry, leaves and the twigs of the plant were collected shade dried and grounded. The solvent extraction of the dried powder of the leaves, berry and the twigs and the fresh berry was carried out in different solvents to get a semi solid extract which was used for further studies like antibacterial activity against E.coli, P.aeruginosa, Klebsiella pneumoniae and S.aureus by well diffusion method where percentage inhibition was calculated using the formula and the graph was plotted. studies of the extract was carried out by DPPH method using UV/Vis spectrophotometer and the percentage scavenging activity of the reading was calculated using the formula and IC 50 value was calculated.

Ultimate Reality and Meaning, 1986

India on account of a large burden of infectious diseases is one amongst the largest consumers of... more India on account of a large burden of infectious diseases is one amongst the largest consumers of antibiotics in the world. Bacterial diseases still play a considerable role in our country. The resistant bacterial strains emerged are spread either through the hospital-acquired infections or from the community. The development of multi drug resistance in pathogenic microbes has put up a huge challenge to find the alternative medicine. Sikkim has a vast repository of diverse plant species where plants species have been claimed as having significant medicinal values. Phyllanthus emblica, commonly known as Indian gooseberry or Amla, is perhaps the most important medicinal plant in the Indian traditional system of medicine. Several parts of these plants are used to treat variety of diseases, but the most important is the fruit. Phyllanthus emblica extracts have been claimed for antibacterial, antifungal, antiviral, anti-inflammatory, antioxidant, cryoprotective, antiaging, nephrotoxicity modulation, antidiabetic, hepatoprotective, antihyperlipidemic, insecticidal, anti-cancer, anti-atherogenic, anti-proliferative, anti-diarrheal, immunomodulator, gastroprotective, cardioprotective, neuroprotective, and radio-protective activity. The current review paper summarizes the morphology, bioactive compounds, antibacterial activity, uses of different solvents and its extracts and their properties, and ethno medicinal uses of the plant Phyllanthus emblica (syn. Emblica officinalis). Different types of extracts and herbal formulations derived from P. emblica have shown tremendous therapeutic and beneficial health effects in countering several diseases and disorders. On the basis of its medicinal properties, various potential remedies can be discovered through the whole plant, making it as an alternate source for the further drug development.

The present investigation was carried out for the determination of genetic conformity of micropro... more The present investigation was carried out for the determination of genetic conformity of micropropagated plants of Zingiber officinale cv Majhauley using Random amplified polymorphic DNA (RAPD) technique.Total fifteen different arbitrary decamers were used as primers to amplify DNA from in vitro plant material to assess the genetic fidelity of 18 months old in vitro cultured plantlets. Total 38 amplified reproducible bands were produced from 10 primers with an average of 3.8 per RAPD primer, amplified bands were ranging from 150-950 base pair, the highest seven number of bands found in primer CLT53 (50-875 bp) and lowest one band in CLT 112 (575 bp) and CLT 192 (650 bp) primers. All the primers were found to be monomorphic and no genetic variation was detected within the micropropagated plants. Present protocol may be used for testing of ginger propagules to ensure mass production of disease free planting materials.

Problems:Molecular information of ginger germplasm of Sikkim varieties is essentialfor characteri... more Problems:Molecular information of ginger germplasm of Sikkim varieties is essentialfor characterization and protection of Sikkim elite ginger germplasm. Experimental approach:The present investigation was carried out for analysis of genetic diversity among five varieties of ginger through RAPD markers. Finding:Total 104 clear, reproducible and scorable fragments ranging from 150-13000 bp were generated from 21 primers and 99% were found polymorphic. Cluster analysis using UPGMA algorithm placed the 40 accessions into three main clusters,the genetic dissimilarity matrix between genotypes ranged from (0.74994-5.33209) between the 5 cultivars studied.The extent of genetic diversity among these cultivars was computed through parameters of gene diversity, sum of allele numbers per locus and Shannon's information indices.Dendrogram was constructed using NTSYSpc, indicating the genetic diversity between five cultivars of ginger using a Jaccard's coefficient. Cluster analysis of dat...

Present investigation was achieved with an efficient, simple micropropagation methods was standar... more Present investigation was achieved with an efficient, simple micropropagation methods was standardized for Ammomum subalatum cv. Ramsey for the production of disease free and rapid multiplication of planting material of Large cardomom micro-propagules and conservation of genetic resources of large cultivator variety of Sikkim. Modified MS medium with 4 % of sucrose with different concentration of hormone showed the suitable medium for multiplication of large cardamom. A total of 52 different hormone concentrations of growth hormone / cultured medium were examined. The maximum number of shoots and roots were observed on (MS+ sucrose 40 g + BAP 3mg/l+0.5 NAA+2mg/l) and second was (MS+ 40g sucrose + BAP 3.5mg/l+0.5 NAA + 2mg/l). After individually subcultured on same fresh medium, adventitious buds were initiated after 10–15 days of culture, complete plantlets developed within 50 days and maintained for 18 months without decreasing the multiplication rate. The combination of cytokinins...

Rhododendron arboreum is one of the renowned Rhododendron plant species of Sikkim. It is vastly k... more Rhododendron arboreum is one of the renowned Rhododendron plant species of Sikkim. It is vastly known for its scenario beauties and remained as attractive flower tree plant. Rhododendron arboreum is medicinally and economically a very important plant species. But, with the unceasing approaches of indecent factors and features the species is under threat in the state. In the study vital research was concluded by contributing an extensive field visit in natural habitat elevations of Sikkim. The morphological studies were conducted along with all the geographical and medicinal parameters. In order to authenticate the database local experts and scientific literatures were equally compared and analyzed. The main objective of the study was to analyzed the significant value of Rhododendron arboreum and promote the importance of this species in the state, which ultimately helps to build a perception of conservative parameter of this species in the state.

Present investigation deals with socio-economic documentation of medicinal plants with potent hyp... more Present investigation deals with socio-economic documentation of medicinal plants with potent hypoglycemic activity. A tribal community of Sikkim (Lepcha, Rai, Limboo, Bhutia, Gurung, Newar etc.) plays an important role in preservation of ethno-traditional knowledge. West Sikkim is highly diversified in term of therapeutic plants species due to its varied ecological and micro zone altitudinal climatic condition. Total 36 species of medicinal plants are recorded. This comprises total of 26 families, 7 shrubs, 18 herbs, 6trees and 3 climbers. Almost all parts of this plant such as leaf, fruit, seed, bark and root could be used to treat elevated glucose level. Simultaneously, scientific research too shows important role in rejuvenate the traditional knowledge and its therapeutic utilization. Current study helps for upcoming researchers to work on all these antidiabetic plants and also persuade the young generation over traditional knowledge, preservation and conservation of potent bio-...

Present investigation was achieved with an efficient, simple micropropagation methods was standar... more Present investigation was achieved with an efficient, simple micropropagation methods was standardized for Ammomum subalatum cv. Ramsey for the production of disease free and rapid multiplication of planting material of Large cardomom micro-propagules and conservation of genetic resources of large cultivator variety of Sikkim. Modified MS medium with 4 % of sucrose with different concentration of hormone showed the suitable medium for multiplication of large cardamom. A total of 52 different hormone concentrations of growth hormone/ cultured medium were examined. The maximum number of shoots and roots were observed on (MS+ sucrose 40 g + BAP 3mg/l+0.5 NAA+2mg/l) and second was (MS+ 40g sucrose + BAP 3.5mg/l+0.5 NAA + 2mg/l). After individually subcultured on same fresh medium, adventitious buds were initiated after 10-15 days of culture, complete plantlets developed within 50 days and maintained for 18 months without decreasing the multiplication rate. The combination of cytokinins and auxins in the culture medium enhanced the response in a number of plants in terms of shoot and root growths, with 100% in vitro multipled plantlets were successfully transferred into field.

Vaccinium sikkimense under the family of Ericaceae is a low bush blueberry commonly found in Nort... more Vaccinium sikkimense under the family of Ericaceae is a low bush blueberry commonly found in Northern area of Sikkim Himalayas. The berry, leaves and the twigs of the plant were collected shade dried and grounded. The solvent extraction of the dried powder of the leaves, berry and the twigs and the fresh berry was carried out in different solvents to get a semi solid extract which was used for further studies like antibacterial activity against E.coli, P.aeruginosa, Klebsiella pneumoniae and S.aureus by well diffusion method where percentage inhibition was calculated using the formula and the graph was plotted. studies of the extract was carried out by DPPH method using UV/Vis spectrophotometer and the percentage scavenging activity of the reading was calculated using the formula and IC 50 value was calculated.

Ginger is a third economically important crop grown after large cardamom and citrus in Sikkim. Th... more Ginger is a third economically important crop grown after large cardamom and citrus in Sikkim. The crop is susceptible to number of pests and disease, the most common disease being rhizome rot caused by Fusarium spp. and Pythium spp. which destroys the yield during cultivation as well as in storage. Farmers are incurring severe losses due to pest and disease for the past many years. However, there is a need to gather more information about pathogens related to rhizome rot, which provides a way to search for more effective prevention and treatment strategies. Infected ginger rhizomes were collected from different parts of Sikkim. The present study reports the association of Fusarium incarnatum (Desm.) Sacc. (synonym: Fusarium semitectum) with rhizome rot of ginger. Morphologically it is floccose with abundant powdery aerial mycelium and brown pigmentation, 3 to 4 septate heterogenous macroconidia of 20 μm. Pathogenicity test revealed 38.48% PI value causing moderate rhizome rot. This...

Hippophae L. is a multipurpose wonder plant found in the Himalayan region which is beneficial bot... more Hippophae L. is a multipurpose wonder plant found in the Himalayan region which is beneficial both ecologically and economically. The genus Hippophae has seven species which are distributed mainly in the Himalayan region. It contains different kinds of nutrients and bioactive substances such as vitamins, carotenoids, flavonoids, polyunsaturated fatty acids, free amino acids and elemental components which have been proved to be very effective for the treatment of various diseases and skin related problems. Not only is it rich in nutrients but it also effectively mitigates many soil related tribulations. It shows multiple pharmacological and therapeutic activities such as antioxidant, immunomodulatory, antiinflammatory, anti-atherogenic, anti-stress, cardio protective and wound healing. Seabuckthorn has attracted attention world over due to its nutritional and medicinal values.

Pharmacognosy magazine, 2017

The main aim of this scientific report was to investigate a series of phytochemicals in silico an... more The main aim of this scientific report was to investigate a series of phytochemicals in silico and the pharmacology of four plants found at higher altitude in the ginger family, Zingiberaceae (incl. Costaceae) from North-East India, particularly Sikkim. First, the goal was to determine the biological activities of the four herbs (used under Zingiberaceae family) using antioxidant assays to identify the best species. Second, previously reported compounds in litero were subsequently screened for their anticancerous activities using in silico methods. Using the methanolic extracts of herbs, quantitative detection of phytochemicals such as total phenols and total flavonoids was detected, and the free radical scavenging activity was also studied using 2,2-diphenyl-1-picryl-hydrazyl (DPPH) assay. Docking process was studied, using Discovery Studio version 3.5, to identify suitable molecules at the protein-binding sites through annealing and genetic simulation algorithms. Grids centered on...

3 Biotech, 2014

Dendrocalamus strictus popularly known as 'Male bamboo' is a multipurpose bamboo which is extensi... more Dendrocalamus strictus popularly known as 'Male bamboo' is a multipurpose bamboo which is extensively utilized in pharmaceutical, paper, agricultural and other industrial implements. In this study, in vitro regeneration of D. strictus through nodal culture has been attempted. Murashige and Skoog's medium supplemented with 4 mg/l BAP was found to be most effective in shoot regeneration with 3.68 ± 0.37 shoots per explant. The effect of Kn was found to be moderate. These hormones also had considerable effect on the shoot length. The highest shoot length after 6 weeks (3.11 ± 0.41 cm) was noted with 5 mg/l BAP followed by 3.07 ± 0.28 cm with 5 mg/l Kn, while decrease in the shoot length was noted with other treatments. The effect of IBA and NAA individually or in combination at different concentrations on rooting was evaluated. The highest number of root (1.36 ± 0.04) was regenerated on full-strength MS medium supplemented with 3 mg/l NAA, while maximum length of 1.64 ± 0.03 cm of roots was recorded with combination of 1 mg/l IBA and 3 mg/l NAA. Tissue-cultured plants thus obtained were successfully transferred to the soil. The clonal fidelity among the in vitro-regenerated plantlets was assessed by RAPD and ISSR markers. The ten RAPD decamers produced 58 amplicons, while nine ISSR primers generated a total of 66 bands. All the bands generated were monomorphic. These results confirmed the clonal fidelity of the tissue culture-raised D. strictus plantlets and corroborated the fact that nodal culture is perhaps the safest mode for multiplication of true to type plants.

Seabuckthorn (Eleagnaceae), used in different countries for the production of several medicines, ... more Seabuckthorn (Eleagnaceae), used in different countries for the production of several medicines, cosmetics and food products. It is rich in vitamin C, vitamin A, E and K, protein, organic acid, carotenoids, flavonoids and steroids. Simultaneously, Hippophae L.is actinorhizal plants and fix N 2 in symbiosis with Frankia. Hippophae salicifolia, H. rhamnoides and H. tibetana occur naturally in the Himalaya, of which H. salicifolia is endemic to Indian Himalaya. Surveys were conducted to study ecology of Hippophae salicifolia in its distribution ranges in Lachen and Lachung valleys of North Sikkim (India). We recorded the occurrence of aerial nodules in Hippophae salicifolia located at Teeling, Lachung. Microscopic observations showed Frankia in the aerial nodules of Hippophae salicifolia. We suggest that development of aerial roots around wound sites and crevices on the trunk (rhytidome) paved the way for aerial nodulation in the species. We report the first aerial nodulation in Hippophae salicifolia from Himalaya.

The present investigation was carried out for the determination of genetic conformity of micropro... more The present investigation was carried out for the determination of genetic conformity of micropropagated plants of Zingiber officinale cv Majhauley using Random amplified polymorphic DNA (RAPD) technique.Total fifteen different arbitrary decamers were used as primers to amplify DNA from in vitro plant material to assess the genetic fidelity of 18 months old in vitro cultured plantlets. Total 38 amplified reproducible bands were produced from 10 primers with an average of 3.8 per RAPD primer, amplified bands were ranging from 150-950 base pair, the highest seven number of bands found in primer CLT53 (50-875 bp) and lowest one band in CLT 112 (575 bp) and CLT 192 (650 bp) primers. All the primers were found to be monomorphic and no genetic variation was detected within the micropropagated plants. Present protocol may be used for testing of ginger propagules to ensure mass production of disease free planting materials.

3 biotech, Aug 9, 2014

Dendrocalamus strictus popularly known as 'Male bamboo' is a multipurpose bamboo which is extensi... more Dendrocalamus strictus popularly known as 'Male bamboo' is a multipurpose bamboo which is extensively utilized in pharmaceutical, paper, agricultural and other industrial implements. In this study, in vitro regeneration of D. strictus through nodal culture has been attempted. Murashige and Skoog's medium supplemented with 4 mg/l BAP was found to be most effective in shoot regeneration with 3.68 ± 0.37 shoots per explant. The effect of Kn was found to be moderate. These hormones also had considerable effect on the shoot length. The highest shoot length after 6 weeks (3.11 ± 0.41 cm) was noted with 5 mg/l BAP followed by 3.07 ± 0.28 cm with 5 mg/l Kn, while decrease in the shoot length was noted with other treatments. The effect of IBA and NAA individually or in combination at different concentrations on rooting was evaluated. The highest number of root (1.36 ± 0.04) was regenerated on full-strength MS medium supplemented with 3 mg/l NAA, while maximum length of 1.64 ± 0.03 cm of roots was recorded with combination of 1 mg/l IBA and 3 mg/l NAA. Tissue-cultured plants thus obtained were successfully transferred to the soil. The clonal fidelity among the in vitro-regenerated plantlets was assessed by RAPD and ISSR markers. The ten RAPD decamers produced 58 amplicons, while nine ISSR primers generated a total of 66 bands. All the bands generated were monomorphic. These results confirmed the clonal fidelity of the tissue culture-raised D. strictus plantlets and corroborated the fact that nodal culture is perhaps the safest mode for multiplication of true to type plants.

Research Journal of Pharmacognosy and Phytochemistry, Dec 1, 2014

Vaccinium sikkimense under the family of Ericaceae is a low bush blueberry commonly found in Nort... more Vaccinium sikkimense under the family of Ericaceae is a low bush blueberry commonly found in Northern area of Sikkim Himalayas. The berry, leaves and the twigs of the plant were collected shade dried and grounded. The solvent extraction of the dried powder of the leaves, berry and the twigs and the fresh berry was carried out in different solvents to get a semi solid extract which was used for further studies like antibacterial activity against E.coli, P.aeruginosa, Klebsiella pneumoniae and S.aureus by well diffusion method where percentage inhibition was calculated using the formula and the graph was plotted. studies of the extract was carried out by DPPH method using UV/Vis spectrophotometer and the percentage scavenging activity of the reading was calculated using the formula and IC 50 value was calculated.

Ultimate Reality and Meaning, 1986

India on account of a large burden of infectious diseases is one amongst the largest consumers of... more India on account of a large burden of infectious diseases is one amongst the largest consumers of antibiotics in the world. Bacterial diseases still play a considerable role in our country. The resistant bacterial strains emerged are spread either through the hospital-acquired infections or from the community. The development of multi drug resistance in pathogenic microbes has put up a huge challenge to find the alternative medicine. Sikkim has a vast repository of diverse plant species where plants species have been claimed as having significant medicinal values. Phyllanthus emblica, commonly known as Indian gooseberry or Amla, is perhaps the most important medicinal plant in the Indian traditional system of medicine. Several parts of these plants are used to treat variety of diseases, but the most important is the fruit. Phyllanthus emblica extracts have been claimed for antibacterial, antifungal, antiviral, anti-inflammatory, antioxidant, cryoprotective, antiaging, nephrotoxicity modulation, antidiabetic, hepatoprotective, antihyperlipidemic, insecticidal, anti-cancer, anti-atherogenic, anti-proliferative, anti-diarrheal, immunomodulator, gastroprotective, cardioprotective, neuroprotective, and radio-protective activity. The current review paper summarizes the morphology, bioactive compounds, antibacterial activity, uses of different solvents and its extracts and their properties, and ethno medicinal uses of the plant Phyllanthus emblica (syn. Emblica officinalis). Different types of extracts and herbal formulations derived from P. emblica have shown tremendous therapeutic and beneficial health effects in countering several diseases and disorders. On the basis of its medicinal properties, various potential remedies can be discovered through the whole plant, making it as an alternate source for the further drug development.

The present investigation was carried out for the determination of genetic conformity of micropro... more The present investigation was carried out for the determination of genetic conformity of micropropagated plants of Zingiber officinale cv Majhauley using Random amplified polymorphic DNA (RAPD) technique.Total fifteen different arbitrary decamers were used as primers to amplify DNA from in vitro plant material to assess the genetic fidelity of 18 months old in vitro cultured plantlets. Total 38 amplified reproducible bands were produced from 10 primers with an average of 3.8 per RAPD primer, amplified bands were ranging from 150-950 base pair, the highest seven number of bands found in primer CLT53 (50-875 bp) and lowest one band in CLT 112 (575 bp) and CLT 192 (650 bp) primers. All the primers were found to be monomorphic and no genetic variation was detected within the micropropagated plants. Present protocol may be used for testing of ginger propagules to ensure mass production of disease free planting materials.

Problems:Molecular information of ginger germplasm of Sikkim varieties is essentialfor characteri... more Problems:Molecular information of ginger germplasm of Sikkim varieties is essentialfor characterization and protection of Sikkim elite ginger germplasm. Experimental approach:The present investigation was carried out for analysis of genetic diversity among five varieties of ginger through RAPD markers. Finding:Total 104 clear, reproducible and scorable fragments ranging from 150-13000 bp were generated from 21 primers and 99% were found polymorphic. Cluster analysis using UPGMA algorithm placed the 40 accessions into three main clusters,the genetic dissimilarity matrix between genotypes ranged from (0.74994-5.33209) between the 5 cultivars studied.The extent of genetic diversity among these cultivars was computed through parameters of gene diversity, sum of allele numbers per locus and Shannon's information indices.Dendrogram was constructed using NTSYSpc, indicating the genetic diversity between five cultivars of ginger using a Jaccard's coefficient. Cluster analysis of dat...

Present investigation was achieved with an efficient, simple micropropagation methods was standar... more Present investigation was achieved with an efficient, simple micropropagation methods was standardized for Ammomum subalatum cv. Ramsey for the production of disease free and rapid multiplication of planting material of Large cardomom micro-propagules and conservation of genetic resources of large cultivator variety of Sikkim. Modified MS medium with 4 % of sucrose with different concentration of hormone showed the suitable medium for multiplication of large cardamom. A total of 52 different hormone concentrations of growth hormone / cultured medium were examined. The maximum number of shoots and roots were observed on (MS+ sucrose 40 g + BAP 3mg/l+0.5 NAA+2mg/l) and second was (MS+ 40g sucrose + BAP 3.5mg/l+0.5 NAA + 2mg/l). After individually subcultured on same fresh medium, adventitious buds were initiated after 10–15 days of culture, complete plantlets developed within 50 days and maintained for 18 months without decreasing the multiplication rate. The combination of cytokinins...

Rhododendron arboreum is one of the renowned Rhododendron plant species of Sikkim. It is vastly k... more Rhododendron arboreum is one of the renowned Rhododendron plant species of Sikkim. It is vastly known for its scenario beauties and remained as attractive flower tree plant. Rhododendron arboreum is medicinally and economically a very important plant species. But, with the unceasing approaches of indecent factors and features the species is under threat in the state. In the study vital research was concluded by contributing an extensive field visit in natural habitat elevations of Sikkim. The morphological studies were conducted along with all the geographical and medicinal parameters. In order to authenticate the database local experts and scientific literatures were equally compared and analyzed. The main objective of the study was to analyzed the significant value of Rhododendron arboreum and promote the importance of this species in the state, which ultimately helps to build a perception of conservative parameter of this species in the state.

Present investigation deals with socio-economic documentation of medicinal plants with potent hyp... more Present investigation deals with socio-economic documentation of medicinal plants with potent hypoglycemic activity. A tribal community of Sikkim (Lepcha, Rai, Limboo, Bhutia, Gurung, Newar etc.) plays an important role in preservation of ethno-traditional knowledge. West Sikkim is highly diversified in term of therapeutic plants species due to its varied ecological and micro zone altitudinal climatic condition. Total 36 species of medicinal plants are recorded. This comprises total of 26 families, 7 shrubs, 18 herbs, 6trees and 3 climbers. Almost all parts of this plant such as leaf, fruit, seed, bark and root could be used to treat elevated glucose level. Simultaneously, scientific research too shows important role in rejuvenate the traditional knowledge and its therapeutic utilization. Current study helps for upcoming researchers to work on all these antidiabetic plants and also persuade the young generation over traditional knowledge, preservation and conservation of potent bio-...

Present investigation was achieved with an efficient, simple micropropagation methods was standar... more Present investigation was achieved with an efficient, simple micropropagation methods was standardized for Ammomum subalatum cv. Ramsey for the production of disease free and rapid multiplication of planting material of Large cardomom micro-propagules and conservation of genetic resources of large cultivator variety of Sikkim. Modified MS medium with 4 % of sucrose with different concentration of hormone showed the suitable medium for multiplication of large cardamom. A total of 52 different hormone concentrations of growth hormone/ cultured medium were examined. The maximum number of shoots and roots were observed on (MS+ sucrose 40 g + BAP 3mg/l+0.5 NAA+2mg/l) and second was (MS+ 40g sucrose + BAP 3.5mg/l+0.5 NAA + 2mg/l). After individually subcultured on same fresh medium, adventitious buds were initiated after 10-15 days of culture, complete plantlets developed within 50 days and maintained for 18 months without decreasing the multiplication rate. The combination of cytokinins and auxins in the culture medium enhanced the response in a number of plants in terms of shoot and root growths, with 100% in vitro multipled plantlets were successfully transferred into field.

Vaccinium sikkimense under the family of Ericaceae is a low bush blueberry commonly found in Nort... more Vaccinium sikkimense under the family of Ericaceae is a low bush blueberry commonly found in Northern area of Sikkim Himalayas. The berry, leaves and the twigs of the plant were collected shade dried and grounded. The solvent extraction of the dried powder of the leaves, berry and the twigs and the fresh berry was carried out in different solvents to get a semi solid extract which was used for further studies like antibacterial activity against E.coli, P.aeruginosa, Klebsiella pneumoniae and S.aureus by well diffusion method where percentage inhibition was calculated using the formula and the graph was plotted. studies of the extract was carried out by DPPH method using UV/Vis spectrophotometer and the percentage scavenging activity of the reading was calculated using the formula and IC 50 value was calculated.

Ginger is a third economically important crop grown after large cardamom and citrus in Sikkim. Th... more Ginger is a third economically important crop grown after large cardamom and citrus in Sikkim. The crop is susceptible to number of pests and disease, the most common disease being rhizome rot caused by Fusarium spp. and Pythium spp. which destroys the yield during cultivation as well as in storage. Farmers are incurring severe losses due to pest and disease for the past many years. However, there is a need to gather more information about pathogens related to rhizome rot, which provides a way to search for more effective prevention and treatment strategies. Infected ginger rhizomes were collected from different parts of Sikkim. The present study reports the association of Fusarium incarnatum (Desm.) Sacc. (synonym: Fusarium semitectum) with rhizome rot of ginger. Morphologically it is floccose with abundant powdery aerial mycelium and brown pigmentation, 3 to 4 septate heterogenous macroconidia of 20 μm. Pathogenicity test revealed 38.48% PI value causing moderate rhizome rot. This...

Hippophae L. is a multipurpose wonder plant found in the Himalayan region which is beneficial bot... more Hippophae L. is a multipurpose wonder plant found in the Himalayan region which is beneficial both ecologically and economically. The genus Hippophae has seven species which are distributed mainly in the Himalayan region. It contains different kinds of nutrients and bioactive substances such as vitamins, carotenoids, flavonoids, polyunsaturated fatty acids, free amino acids and elemental components which have been proved to be very effective for the treatment of various diseases and skin related problems. Not only is it rich in nutrients but it also effectively mitigates many soil related tribulations. It shows multiple pharmacological and therapeutic activities such as antioxidant, immunomodulatory, antiinflammatory, anti-atherogenic, anti-stress, cardio protective and wound healing. Seabuckthorn has attracted attention world over due to its nutritional and medicinal values.

Pharmacognosy magazine, 2017

The main aim of this scientific report was to investigate a series of phytochemicals in silico an... more The main aim of this scientific report was to investigate a series of phytochemicals in silico and the pharmacology of four plants found at higher altitude in the ginger family, Zingiberaceae (incl. Costaceae) from North-East India, particularly Sikkim. First, the goal was to determine the biological activities of the four herbs (used under Zingiberaceae family) using antioxidant assays to identify the best species. Second, previously reported compounds in litero were subsequently screened for their anticancerous activities using in silico methods. Using the methanolic extracts of herbs, quantitative detection of phytochemicals such as total phenols and total flavonoids was detected, and the free radical scavenging activity was also studied using 2,2-diphenyl-1-picryl-hydrazyl (DPPH) assay. Docking process was studied, using Discovery Studio version 3.5, to identify suitable molecules at the protein-binding sites through annealing and genetic simulation algorithms. Grids centered on...

3 Biotech, 2014

Dendrocalamus strictus popularly known as 'Male bamboo' is a multipurpose bamboo which is extensi... more Dendrocalamus strictus popularly known as 'Male bamboo' is a multipurpose bamboo which is extensively utilized in pharmaceutical, paper, agricultural and other industrial implements. In this study, in vitro regeneration of D. strictus through nodal culture has been attempted. Murashige and Skoog's medium supplemented with 4 mg/l BAP was found to be most effective in shoot regeneration with 3.68 ± 0.37 shoots per explant. The effect of Kn was found to be moderate. These hormones also had considerable effect on the shoot length. The highest shoot length after 6 weeks (3.11 ± 0.41 cm) was noted with 5 mg/l BAP followed by 3.07 ± 0.28 cm with 5 mg/l Kn, while decrease in the shoot length was noted with other treatments. The effect of IBA and NAA individually or in combination at different concentrations on rooting was evaluated. The highest number of root (1.36 ± 0.04) was regenerated on full-strength MS medium supplemented with 3 mg/l NAA, while maximum length of 1.64 ± 0.03 cm of roots was recorded with combination of 1 mg/l IBA and 3 mg/l NAA. Tissue-cultured plants thus obtained were successfully transferred to the soil. The clonal fidelity among the in vitro-regenerated plantlets was assessed by RAPD and ISSR markers. The ten RAPD decamers produced 58 amplicons, while nine ISSR primers generated a total of 66 bands. All the bands generated were monomorphic. These results confirmed the clonal fidelity of the tissue culture-raised D. strictus plantlets and corroborated the fact that nodal culture is perhaps the safest mode for multiplication of true to type plants.

Seabuckthorn (Eleagnaceae), used in different countries for the production of several medicines, ... more Seabuckthorn (Eleagnaceae), used in different countries for the production of several medicines, cosmetics and food products. It is rich in vitamin C, vitamin A, E and K, protein, organic acid, carotenoids, flavonoids and steroids. Simultaneously, Hippophae L.is actinorhizal plants and fix N 2 in symbiosis with Frankia. Hippophae salicifolia, H. rhamnoides and H. tibetana occur naturally in the Himalaya, of which H. salicifolia is endemic to Indian Himalaya. Surveys were conducted to study ecology of Hippophae salicifolia in its distribution ranges in Lachen and Lachung valleys of North Sikkim (India). We recorded the occurrence of aerial nodules in Hippophae salicifolia located at Teeling, Lachung. Microscopic observations showed Frankia in the aerial nodules of Hippophae salicifolia. We suggest that development of aerial roots around wound sites and crevices on the trunk (rhytidome) paved the way for aerial nodulation in the species. We report the first aerial nodulation in Hippophae salicifolia from Himalaya.