Sweeta Manhas - Academia.edu (original) (raw)

Papers by Sweeta Manhas

Research paper thumbnail of Isolation and Identification of L-asparaginase Producing Bacteria from Soils of Different Agroclimatic Zones of Jammu (J&K), India

International Journal of Plant & Soil Science

A milestone was set in the field of medicine with the discovery of L-asparaginase present in the ... more A milestone was set in the field of medicine with the discovery of L-asparaginase present in the serum of guinea pig responsible for action against the acute lymphoblastic leukemia. Since then, the use of L-asparaginase as a potential anti-cancer drug has been a great success in the field of modern oncology. Despite its varied sources, new sources are continuously being explored to obtain a high therapeutic index drug. The study aims to isolate potential L-asparaginase producing microorganisms from environmental niches of Northwestern Himalayas. The climatic conditions of the region and ancient agricultural practices which are still being followed in these areas, add up to the diverse microbial repository and a potential habitat which can be explored to obtain a potent asparaginase producing microbial source. In the study, soil samples from different regions of Jammu were collected aseptically. From the isolation studies, a total of 44 bacterial isolates were obtained. Pure cultures...

Research paper thumbnail of Plant Oxylipins

CRC Press eBooks, Jan 30, 2023

Research paper thumbnail of The Role of Oxylipins in Abiotic Stress Resistance

CRC Press eBooks, Jan 30, 2023

Research paper thumbnail of Optimization of L-asparaginase from Enterobacter aesburiae via response surface approach

L-asparaginase, an important biopharmaceutical, has been a boon to cancer patients, especially fo... more L-asparaginase, an important biopharmaceutical, has been a boon to cancer patients, especially for the therapeutic treatment of acute lymphoblastic leukemia. However, asparaginase formulations from E. coli and Erwinia species currently being used are associated with potential side effects causing hindrances towards a successful therapeutic treatment. Therefore, optimization and production of asparaginase from varied microbial sources has been the aim of several studies to overcome the hypersensitive and toxicological responses associated with presently used drug formulations. In this study, the possibility of using Enterobacter aesburiae strain R16C1/MT93543isolated from black gram rhizospheric soil sample as L-asparaginase source of industrial importance, was investigated. Different fermentation process parameters for the production of enzyme were optimized using submerged fermentation in shake flask. Box Behnken design was used to optimize and study individual as well as interacti...

Research paper thumbnail of Production and Purification of L-asparaginase obtained from a Bacterial Isolate of Black Gram Rhizospheric Soil at J&K, India

Journal of Natural Resource Conservation and Management

Research paper thumbnail of Spectrum of Mucormycosis Before and During COVID-19: Epidemiology, Diagnosis, and Current Therapeutic Interventions

Current Fungal Infection Reports

Purpose of Review More than half a billion people have been infected and 6.2 million killed by th... more Purpose of Review More than half a billion people have been infected and 6.2 million killed by the severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) since the start of the pandemic in 2019. Systemic glucocorticoids are a double-edged sword, on the one hand, life-saving in treating COVID-19 complications while on the other hand, potentially leading to life-and-limb-threatening opportunistic fungal infections. Mucormycosis (MM) is caused by the mucormycetes family. Although rare, it is characterized by high mortality and significant morbidity. The gross similarities observed with other fungal infections which respond to different treatment regimens have made it all the more imperative to quickly and sensitively diagnose and treat MM. This review discusses the epidemiology of MM before and during the COVID-19 pandemic, associated risk factors, COVID-19-associated MM, diagnosis, and current therapeutic interventions. Recent Findings There has been a widespread and worrisome trend of rising in cases of MM, worldwide, but more so in the Indian subcontinent, where it is nicknamed the "black fungus." This upsurge has picked up the pace ever since the start of the COVID-19 pandemic. Necrosis is secondary to the angio-invasive and pro-thrombotic nature of the mold resulting in extensive lesions presenting mostly as rhino-orbital MM (ROM) and rhino-orbito-cerebral MM (ROCM). Infection is mostly observed in subjects with underlying risk factors such as uncontrolled diabetes, those receiving hematopoietic stem cell transplant, and/or on corticosteroid or immunosuppressive therapy, although it is widely suspected that other factors such as iron and zinc may play a role in the pathogenesis of MM. The "One world one guideline" strategy advocates both prophylactic anti-fungal therapy along with aggressive, prompt, and individualized treatment with anti-fungal drugs such as amphotericin B in addition to vigorous surgical intervention. High-risk groups need particularly rapid diagnosis although empirical antifungal therapy may not be delayed. Speeding diagnostic turnaround times are essential to institute early therapy, and there is much scope for newer modalities such as PCR, matrix-assisted laser desorption ionization-time of flight mass spectrometry, and whole-genome sequencing in such endeavors. The results of strict monitoring of blood glucose levels along with rational and limited use of steroids and immunomodulatory drugs have proven to be a significant preventive measure. Summary The significant rise in cases of MM worldwide has necessitated viewing each case with a strong index of suspicion. Adoption of rapid diagnostics, early antifungal therapy, and prompt surgical interventions are essential, while high-risk groups need particular focused care which may include prophylactic anti-fungal therapy, limited steroid use, and meticulous control of the underlying disease. Developing quicker and more sensitive diagnostic modalities has great potential to improve the detection and management of MM.

Research paper thumbnail of Entomopathogenic Nematodes and Their Efficiency in Different Host

The term „Entomophilic nematodes‟ includes all relationships of insects and nematodes ranging fro... more The term „Entomophilic nematodes‟ includes all relationships of insects and nematodes ranging from phoresis to parasitism and pathogenesis. „Entomogenous nematodes‟ are those that have a facultative or obligate parasitic association with insects. Entomogenous nematodes have several deleterious effects on their hosts including sterility, reduced fecundity, longevity and flight activity, delayed development, or other behavioral, physiological and morphological aberrations and in some cases, rapid mortality. The entomopathogenic nematodes possessing balanced biological control attributes belong to genera Steinernema and Heterorhabditis and are having mutualistic association with bacteria of the genus Xenorhabdus for Steinernematidae and Photorhabdus for Heterorhabditidae. Entomopathogenic nematodes being highly lethal to many important insect-pests, are safe to nontargetorganisms and working with their symbiotic bacteria kill the insects within 24-28 hours as compared to days and weeks...

Research paper thumbnail of Reactive Oxygen Species in Plants: From Source to Sink

Antioxidants

Reactive oxygen species (ROS, partial reduction or derivatives of free radicals) are highly react... more Reactive oxygen species (ROS, partial reduction or derivatives of free radicals) are highly reactive, dangerous and can cause oxidative cell death. In addition to their role as toxic by-products of aerobic metabolism, ROS play a role in the control and regulation of biological processes such as growth, the cell cycle, programmed cell death, hormone signaling, biotic and abiotic stress reactions and development. ROS always arise in plants as a by-product of several metabolic processes that are located in different cell compartments, or as a result of the inevitable escape of electrons to oxygen from the electron transport activities of chloroplasts, mitochondria and plasma membranes. These reactive species are formed in chloroplasts, mitochondria, plasma membranes, peroxisomes, apoplasts, the endoplasmic reticulum and cell walls. The action of many non-enzymatic and enzymatic antioxidants present in tissues is required for efficient scavenging of ROS generated during various environm...

Research paper thumbnail of Physico-biochemical evaluation of certain promising varieties of chickpea (Cicer arietinum L.) grown along the banks of Ganga River in Uttar Pradesh

An experiment was conducted during Rabi season of 2015-16 at Oilseed Research Farm, Kalyanpur, C.... more An experiment was conducted during Rabi season of 2015-16 at Oilseed Research Farm, Kalyanpur, C.S.A University of Agriculture and Technology, Kanpur to study the biochemical aspects of certain promising varieties of chickpea. Results revealed that the dahl protein content varied from 21-24.1 per cent in different varieties of chickpea. Significantly highest protein content in dahl was obtained in chickpea variety KWR-108 compared to rest varieties of chickpea. The recovery in whole grain in different varieties of chickpea ranged from 68-87 per cent. The chickpea variety Pragati recorded maximum dahl recovery of 87 per cent in whole grain followed by Radhey, KPG-59, Avarodhi, RSG-963, KWR-108 and BG-372. Different varieties of chickpea showed variation in husk per cent from 11.42-16.42 per cent. The variety Pragati gave lower mean value for husk then the other varieties of chickpea. Broken dahl recovery ranged from 0.49-5.28 per cent and highest per cent obtained in variety Pusa-256...

Research paper thumbnail of Phytoremediation at Molecular Level

Research paper thumbnail of Biochar as a tool for effective management of drought and heavy metal toxicity

Research paper thumbnail of Covid-19 Pandemic and Current Medical Interventions

Archives of Medical Research

Research paper thumbnail of Effect of long term application of organic and inorganic nutrient sources on soil moisture and yield of maize under rainfed conditions

Journal of Soil and Water Conservation, 2016

Research paper thumbnail of Isolation and Identification of L-asparaginase Producing Bacteria from Soils of Different Agroclimatic Zones of Jammu (J&K), India

International Journal of Plant & Soil Science

A milestone was set in the field of medicine with the discovery of L-asparaginase present in the ... more A milestone was set in the field of medicine with the discovery of L-asparaginase present in the serum of guinea pig responsible for action against the acute lymphoblastic leukemia. Since then, the use of L-asparaginase as a potential anti-cancer drug has been a great success in the field of modern oncology. Despite its varied sources, new sources are continuously being explored to obtain a high therapeutic index drug. The study aims to isolate potential L-asparaginase producing microorganisms from environmental niches of Northwestern Himalayas. The climatic conditions of the region and ancient agricultural practices which are still being followed in these areas, add up to the diverse microbial repository and a potential habitat which can be explored to obtain a potent asparaginase producing microbial source. In the study, soil samples from different regions of Jammu were collected aseptically. From the isolation studies, a total of 44 bacterial isolates were obtained. Pure cultures...

Research paper thumbnail of Plant Oxylipins

CRC Press eBooks, Jan 30, 2023

Research paper thumbnail of The Role of Oxylipins in Abiotic Stress Resistance

CRC Press eBooks, Jan 30, 2023

Research paper thumbnail of Optimization of L-asparaginase from Enterobacter aesburiae via response surface approach

L-asparaginase, an important biopharmaceutical, has been a boon to cancer patients, especially fo... more L-asparaginase, an important biopharmaceutical, has been a boon to cancer patients, especially for the therapeutic treatment of acute lymphoblastic leukemia. However, asparaginase formulations from E. coli and Erwinia species currently being used are associated with potential side effects causing hindrances towards a successful therapeutic treatment. Therefore, optimization and production of asparaginase from varied microbial sources has been the aim of several studies to overcome the hypersensitive and toxicological responses associated with presently used drug formulations. In this study, the possibility of using Enterobacter aesburiae strain R16C1/MT93543isolated from black gram rhizospheric soil sample as L-asparaginase source of industrial importance, was investigated. Different fermentation process parameters for the production of enzyme were optimized using submerged fermentation in shake flask. Box Behnken design was used to optimize and study individual as well as interacti...

Research paper thumbnail of Production and Purification of L-asparaginase obtained from a Bacterial Isolate of Black Gram Rhizospheric Soil at J&K, India

Journal of Natural Resource Conservation and Management

Research paper thumbnail of Spectrum of Mucormycosis Before and During COVID-19: Epidemiology, Diagnosis, and Current Therapeutic Interventions

Current Fungal Infection Reports

Purpose of Review More than half a billion people have been infected and 6.2 million killed by th... more Purpose of Review More than half a billion people have been infected and 6.2 million killed by the severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) since the start of the pandemic in 2019. Systemic glucocorticoids are a double-edged sword, on the one hand, life-saving in treating COVID-19 complications while on the other hand, potentially leading to life-and-limb-threatening opportunistic fungal infections. Mucormycosis (MM) is caused by the mucormycetes family. Although rare, it is characterized by high mortality and significant morbidity. The gross similarities observed with other fungal infections which respond to different treatment regimens have made it all the more imperative to quickly and sensitively diagnose and treat MM. This review discusses the epidemiology of MM before and during the COVID-19 pandemic, associated risk factors, COVID-19-associated MM, diagnosis, and current therapeutic interventions. Recent Findings There has been a widespread and worrisome trend of rising in cases of MM, worldwide, but more so in the Indian subcontinent, where it is nicknamed the "black fungus." This upsurge has picked up the pace ever since the start of the COVID-19 pandemic. Necrosis is secondary to the angio-invasive and pro-thrombotic nature of the mold resulting in extensive lesions presenting mostly as rhino-orbital MM (ROM) and rhino-orbito-cerebral MM (ROCM). Infection is mostly observed in subjects with underlying risk factors such as uncontrolled diabetes, those receiving hematopoietic stem cell transplant, and/or on corticosteroid or immunosuppressive therapy, although it is widely suspected that other factors such as iron and zinc may play a role in the pathogenesis of MM. The "One world one guideline" strategy advocates both prophylactic anti-fungal therapy along with aggressive, prompt, and individualized treatment with anti-fungal drugs such as amphotericin B in addition to vigorous surgical intervention. High-risk groups need particularly rapid diagnosis although empirical antifungal therapy may not be delayed. Speeding diagnostic turnaround times are essential to institute early therapy, and there is much scope for newer modalities such as PCR, matrix-assisted laser desorption ionization-time of flight mass spectrometry, and whole-genome sequencing in such endeavors. The results of strict monitoring of blood glucose levels along with rational and limited use of steroids and immunomodulatory drugs have proven to be a significant preventive measure. Summary The significant rise in cases of MM worldwide has necessitated viewing each case with a strong index of suspicion. Adoption of rapid diagnostics, early antifungal therapy, and prompt surgical interventions are essential, while high-risk groups need particular focused care which may include prophylactic anti-fungal therapy, limited steroid use, and meticulous control of the underlying disease. Developing quicker and more sensitive diagnostic modalities has great potential to improve the detection and management of MM.

Research paper thumbnail of Entomopathogenic Nematodes and Their Efficiency in Different Host

The term „Entomophilic nematodes‟ includes all relationships of insects and nematodes ranging fro... more The term „Entomophilic nematodes‟ includes all relationships of insects and nematodes ranging from phoresis to parasitism and pathogenesis. „Entomogenous nematodes‟ are those that have a facultative or obligate parasitic association with insects. Entomogenous nematodes have several deleterious effects on their hosts including sterility, reduced fecundity, longevity and flight activity, delayed development, or other behavioral, physiological and morphological aberrations and in some cases, rapid mortality. The entomopathogenic nematodes possessing balanced biological control attributes belong to genera Steinernema and Heterorhabditis and are having mutualistic association with bacteria of the genus Xenorhabdus for Steinernematidae and Photorhabdus for Heterorhabditidae. Entomopathogenic nematodes being highly lethal to many important insect-pests, are safe to nontargetorganisms and working with their symbiotic bacteria kill the insects within 24-28 hours as compared to days and weeks...

Research paper thumbnail of Reactive Oxygen Species in Plants: From Source to Sink

Antioxidants

Reactive oxygen species (ROS, partial reduction or derivatives of free radicals) are highly react... more Reactive oxygen species (ROS, partial reduction or derivatives of free radicals) are highly reactive, dangerous and can cause oxidative cell death. In addition to their role as toxic by-products of aerobic metabolism, ROS play a role in the control and regulation of biological processes such as growth, the cell cycle, programmed cell death, hormone signaling, biotic and abiotic stress reactions and development. ROS always arise in plants as a by-product of several metabolic processes that are located in different cell compartments, or as a result of the inevitable escape of electrons to oxygen from the electron transport activities of chloroplasts, mitochondria and plasma membranes. These reactive species are formed in chloroplasts, mitochondria, plasma membranes, peroxisomes, apoplasts, the endoplasmic reticulum and cell walls. The action of many non-enzymatic and enzymatic antioxidants present in tissues is required for efficient scavenging of ROS generated during various environm...

Research paper thumbnail of Physico-biochemical evaluation of certain promising varieties of chickpea (Cicer arietinum L.) grown along the banks of Ganga River in Uttar Pradesh

An experiment was conducted during Rabi season of 2015-16 at Oilseed Research Farm, Kalyanpur, C.... more An experiment was conducted during Rabi season of 2015-16 at Oilseed Research Farm, Kalyanpur, C.S.A University of Agriculture and Technology, Kanpur to study the biochemical aspects of certain promising varieties of chickpea. Results revealed that the dahl protein content varied from 21-24.1 per cent in different varieties of chickpea. Significantly highest protein content in dahl was obtained in chickpea variety KWR-108 compared to rest varieties of chickpea. The recovery in whole grain in different varieties of chickpea ranged from 68-87 per cent. The chickpea variety Pragati recorded maximum dahl recovery of 87 per cent in whole grain followed by Radhey, KPG-59, Avarodhi, RSG-963, KWR-108 and BG-372. Different varieties of chickpea showed variation in husk per cent from 11.42-16.42 per cent. The variety Pragati gave lower mean value for husk then the other varieties of chickpea. Broken dahl recovery ranged from 0.49-5.28 per cent and highest per cent obtained in variety Pusa-256...

Research paper thumbnail of Phytoremediation at Molecular Level

Research paper thumbnail of Biochar as a tool for effective management of drought and heavy metal toxicity

Research paper thumbnail of Covid-19 Pandemic and Current Medical Interventions

Archives of Medical Research

Research paper thumbnail of Effect of long term application of organic and inorganic nutrient sources on soil moisture and yield of maize under rainfed conditions

Journal of Soil and Water Conservation, 2016