Sweta Prasad - Academia.edu (original) (raw)
Uploads
Papers by Sweta Prasad
In Vitro Cellular & Developmental Biology - Animal, 2006
The proliferative capacity of T cells iu respouse to various stimuli is commonly determiued by ra... more The proliferative capacity of T cells iu respouse to various stimuli is commonly determiued by radioactive assay based on incorporation of [3H]thynlidine ([3H]TdR) into newly synthesized DNA. Ill order to assess techniques for application in laboratories where radioactive facilities are not present, all alternative method was tested. As all alternative, T-cell proliferation was rneasured by spectrophotonletrically analyzing, the presence of all enzyme adenosine deammase ill lyn|phocytes and also using a standard XTI" assay. Jurkat (human) T-cell line (chine EO.1) was used for lymphocyte population. Tile Jurkat cell concentration was adjusted according to different cell densities and enzyme actMty was determined. Cells were also seeded ill complete mediunl up to 72 h and han'ested for estimation of enzyme actMty. A significant correlation between the standard cell-proliferation assay and adenosine deaminase assay was observed. The present study indicates that the assay of adenosine deaminase is a reliable and accurate method for measuring proliferation of T lynThocytes.
In Vitro Cellular & Developmental Biology - Animal, 2006
The proliferative capacity of T cells iu respouse to various stimuli is commonly determiued by ra... more The proliferative capacity of T cells iu respouse to various stimuli is commonly determiued by radioactive assay based on incorporation of [3H]thynlidine ([3H]TdR) into newly synthesized DNA. Ill order to assess techniques for application in laboratories where radioactive facilities are not present, all alternative method was tested. As all alternative, T-cell proliferation was rneasured by spectrophotonletrically analyzing, the presence of all enzyme adenosine deammase ill lyn|phocytes and also using a standard XTI" assay. Jurkat (human) T-cell line (chine EO.1) was used for lymphocyte population. Tile Jurkat cell concentration was adjusted according to different cell densities and enzyme actMty was determined. Cells were also seeded ill complete mediunl up to 72 h and han'ested for estimation of enzyme actMty. A significant correlation between the standard cell-proliferation assay and adenosine deaminase assay was observed. The present study indicates that the assay of adenosine deaminase is a reliable and accurate method for measuring proliferation of T lynThocytes.