Syahruddin Said - Academia.edu (original) (raw)
Papers by Syahruddin Said
Veterinary World, Jan 14, 2025
Background and Aim: Bali-polled bulls, known for their favorable traits, require advanced reprodu... more Background and Aim: Bali-polled bulls, known for their favorable traits, require advanced reproductive performance analysis to optimize breeding programs. This study aimed to evaluate sperm kinematics and seminal plasma proteomic profiles as biomarkers for sperm motility and fertility in Bali-polled bulls.
Materials and Methods: Semen from five Bali-polled bulls was collected biweekly over five batches using artificial vaginas. Sperm kinematics were assessed using computer-assisted semen analysis (CASA). Fertility was evaluated through service per conception (S/C) in artificial insemination trials. Seminal plasma proteins were analyzed through liquid chromatography–tandem mass spectrometry (LC-MS/MS) and annotated using the UniProt database, PANTHER for gene ontology, and STRING database for protein interactions.
Results: Post-thaw sperm kinematics showed satisfactory results, with a mean S/C of 1.52. Proteomic analysis identified 138 proteins, including six (TEX101, BSP1, PRSS55, BSP3, SPADH2, and TPPP2) linked to sperm motility. These proteins were involved in key biological processes such as sperm capacitation, motility regulation, and sperm-oocyte interaction.
Conclusion: Sperm kinematics and seminal plasma proteomics provide insights into Bali-polled bull fertility. Identified proteins can serve as fertility biomarkers, aiding in superior local breed development and reproductive efficiency improvement.
Keywords: artificial insemination, Bali-polled bulls, fertility biomarkers, seminal plasma proteomics, sperm kinematics.
Indian Journal of Animal Sciences, Jan 6, 2021
Livestock and Animal Research
Objective: The purpose of this research is to determine the effect of the rainy and dry seasons o... more Objective: The purpose of this research is to determine the effect of the rainy and dry seasons on the sperm qualities and frozen sperm production of Bali bulls at the Lelede Regional Artificial Insemination Center (RAIC) in West Nusa Tenggara.Methods: A total of 1226 ejaculates were examined from five Bali bulls during a 24-month period (November 2018–October 2019), which was classified into rainy (November–April, 658 ejaculates) and dry (May–October, 568 ejaculates) seasons. Semen volume (mL), sperm velocity (1-4), motility (%), concentration (x106 cell/ml), pre-freezing motility (%), post-freezing motility (%), recovery rate (%), and total frozen semen production were examined as sperm quality measures.Results: The results indicated that during the rainy season, ejaculates and processed sperm were more abundant than during the dry season. Additionally, the features of discarded fresh semen in the volume parameter were significantly (P<0.05) larger during the dry season than du...
Journal of the Indonesian Tropical Animal Agriculture, 2016
Telah dilakukan suatu penelitian untuk mengidentifikasi variasi gen myostatin dan mengevaluasi ke... more Telah dilakukan suatu penelitian untuk mengidentifikasi variasi gen myostatin dan mengevaluasi keberadaan marker genetik untuk sifat double muscling (delesi 11 basa pada ekson 3 gen myostatin) pada generasi pertama (F1) hasil persilangan dengan sapi Belgian Blue pertama di Indonesia. Sebanyak 8 sampel DNA sapi yang dipelihara di PT. Karya Anugerah Rumpin (KAR) digunakan dalam penelitian ini. Analisa Single Strand Conformation Polymorphism (SSCP) dan sekuensing digunakan untuk mengidentifikasi keragaman dari gen myostatin. Terdapat 3 tipe alel gen myostatin berdasarkan hasil analisa SSCP. Pejantan Belgian Blue memiliki tipe alel A. Sapi Simmental, Wagyu, SO x BX, Charolais, dan sapi PO memiliki tipe alel B. Sementara itu anak sapi hasil perkawinan Belgian Blue x FH dan Belgian Blue x SO memiliki tipe alel C (heterozigot). Berdasarkan hasil analisa sekuen gen myostatin, ditemukan adanya delesi 11 basa pada ekson 3 gen myostatin pejantan Belgian Blue. Hasil analisa sekuensing juga memberikan informasi bahwa generasi F1 (Belgian Blue x FH dan Belgian Blue x SO) memiliki gen myostatin yang berada dalam kondisi heterozigot. Hasil penelitian ini memberikan bukti ilmiah bahwa delesi 11 basa pada ekson 3 gen myostatin yang merupakan marker untuk sifat double muscling pada sapi Belgian Blue juga ada (diwariskan) pada anak-anak sapi generasi F1 (jantan dan betina).
Journal of the Indonesian Tropical Animal Agriculture, 2014
Penelitian ini bertujuan untuk menentukan angka kebuntingan dan kelahiran sapi Bali sesudah sinkr... more Penelitian ini bertujuan untuk menentukan angka kebuntingan dan kelahiran sapi Bali sesudah sinkronisasi estrus dan inseminasi buatan (IB) dengan semen beku sexing serta kesesuaian jenis kelamin anak. Penelitian dilakukan pada dua lokasi peternakan di Kabupaten Lombok Barat. Sinkronisasi estrus dilakukan melalui pemberian satu suntikan 5 cc Lutalyse ® yang mengandung 25 mg PGF 2α (Lutalyse, Sterile Solution, Pharmacia Animal Health) secara intra muscular. Pengamatan dilakukan pada sapi yang memberikan respon dan diinseminasi pada saat estrus. IB juga langsung dilakukan pada sapi-sapi yang menunjukkan birahi alami. Diagnosa kebuntingan dilakukan melalui palpasi rektal pada hari ke-90. Pada Peternakan 1, angka kebuntingan sapi-sapi yang mendapat perlakuan dan yang tidak mendapat perlakuan PGF 2α masing-masing sebesar 29/63 (46,03%) dan 71/75 (94,66%). Pada Peternakan 2, nilainilai yang bersesuaian masing-masing adalah 38/89 (42,70%) dan 10/23 (43,48%). Angka kelahiran anak pada kedua farm diperoleh 100%. Angka kesesuaian jenis kelamin untuk anak betina yang lahir di Peternakan 1 adalah 94.00%, sedangkan di Peternakan 2, untuk anak sapi yang terlahir jantan adalah 93.75%. Dibawah kondisi penelitian ini, sinkronisasi estrus yang diikuti dengan IB semen beku hasil sexing tampaknya dapat diaplikasikan di lapang sehingga menghasilkan angka kebuntingan dan kelahiran anak yang pantas diterima, serta angka kesesuaian di atas 90% untuk masing-masing jenis kelamin betina maupun jantan. Kata kunci: inseminasi buatan, sapi Bali, sinkronisasi estrus, semen beku-sexing, angka kesesuaian jenis kelamin
The presented study was aimed at determining the association of growth hormone (GH) gene polymorp... more The presented study was aimed at determining the association of growth hormone (GH) gene polymorphism with the traits that characterise carcass and meat quality and meat basic chemical composition in porkers with genotype CC with respect to RYR1 locus. The study was carried out on 126 PIC porkers originating after Camborough 22 sows and PIC 337 boars. The polymorphism of GH gene was identified respectively with restrictive enzymes MspI, HaeII, CfoI and ApaI. The allele frequencies were as follows: GH/MspI A = 0.78 and B = 0.22; GH/HaeII A = 0.86 and B = 0.14; GH/CfoI C1 = 0.09 and C2 = 0.03 and C3 = 0.18 and C4 = 0.70; GH/ApaI A1 = 0.28 and A2 = 0.72. The analysis of association of GH genotypes identified with four restrictive enzymes did not show any relationship between the GH gene polymorphism and the carcass slaughter traits determined with meatiness measuring apparatus CGM and the meat quality and meat basic chemical composition in PIC hybrid porkers. It may be stated, basing o...
APCBEE Procedia, 2014
Abstract The aim of this research was to determine the nutrient content, digestibility on wafer o... more Abstract The aim of this research was to determine the nutrient content, digestibility on wafer of feed supplement and productivity of Bali calves. This research had two steps experimental, the first steps experiment to determine the nutrient content and digestibility on wafer of feed supplement. The composition wafer of feed supplement i.e. T1 = wafer containing lamtoro leaf, T2 = wafer containing lamtoro and papaya leaf, T3 = wafer containing moringa leaf, T4 = wafer containing gamal leaf, T5= wafer containing corn leaf and corn. Nutrient content and digestibility of T1 had highest compared among the others, i.e. crude protein (32.34%), ash (7.24%), crude fiber (16.85%), crude fat (4.52%), NFE (39.06 cal/g), digestibility of dry matter 82.87%, digestibility of organic matter 81.78%, NH3 9.33% and VFA 164.55%. The second steps of this research was the best result of the nutrient content and digestibility of wafer of feed supplements tested to bali calves. Level of wafer containing lamtoro leaf,i.e R1 =0%, R2 =5%, R3 =10%, R4=15%. The result indicated that treatments had significant effect (P
Journal of the Indonesian Tropical Animal Agriculture, 2016
Selection of livestock was purposed to increase productivity. A performance test was conducted us... more Selection of livestock was purposed to increase productivity. A performance test was conducted using 25 males and 25 females of Sumba Ongole (SO) cattle. The performance test were conducted in three periods (2014, 2015 and 2016) for about 535 days. Descriptive statistics ws used to analyze data of body weight. Cattle with age between 300 to 600 days were used for evaluation. Result showed that heritability value of yearling weight (YW365) was 0.77+0.68 as a high category. The highest breeding value of YW365 were 66.05 kg (male) and 41.89 kg (female). The average of corrected final weight (CFW) weight were 172.55+34.22 kg (male) and 159.80+37.73 kg (female). The performance test in this study obtained six A class (1 bull and 5 cows) based on the standard minimum of body measurements for SO cattle.
Veterinary World
Background and Aim: Capacity for sperm production is affected by age, which is related to the mor... more Background and Aim: Capacity for sperm production is affected by age, which is related to the morphology of sperm abnormalities and can affect fertility. The aim of this study was to evaluate the relationship between age and concentrations of testosterone and adiponectin with sperm abnormalities in Simmental bulls. Materials and Methods: The study used 11 bulls, separated into three groups. The first group consisted of five bulls aged 4-5 years, and the second and third groups each consisted of three bulls, aged 6-7 and 8-10 years, respectively. The average sperm motility of the animals ranged from 57.66±2.60% to 70.17±0.22%. Blood samples were obtained from the coccygeal region of the animals. Testosterone and adiponectin analysis was performed using the enzyme-linked immunosorbent assay method. Sperm morphology was evaluated using carbol fuchsin-eosin staining according to the Williams method. Finally, correlations between testosterone and adiponectin concentrations, age, and sper...
Journal of the Indonesian Tropical Animal Agriculture
The objective of the current study was to investigate the effect of addition an amino acid or its... more The objective of the current study was to investigate the effect of addition an amino acid or its combination with EDTA on DNA integrity and morphometry sperm heads of freeze-dried bovine spermatozoa. Spermatozoa were freeze-dried in medium with the addition of an amino acid glycine, cysteine, glutamine, or its combination with EDTA. Sperm head morphometry was identified at 400X magnification using Axio Vision(Zeiss Company, Germany), while for membrane plasma integrity (MPI) determined by calculation of the percentage of spermatozoa having intact plasma membrane by osmotic resistance test method and DNA integrity analysis using acridine orange staining. Sperm head had declined in size after the freeze-drying process, MPI of FD spermatozoa gradually increased significantly when FD solution was added with an amino acid solution (glycine, cysteine) and its combination with EDTA. DNA integrity of all freeze-dried spermatozoa treatments was remaining intact, no significantly different (...
Buletin Peternakan
The Thyroglobulin (TG) and Leptin (LEP) genes are two candidate genes that widely used for molecu... more The Thyroglobulin (TG) and Leptin (LEP) genes are two candidate genes that widely used for molecular selection to improve carcass traits in beef cattle. This research was carried out to identify the genetic characterization of TG and LEP genes from 47 heads of Pasundan cows at West Java using PCR-RFLP method. Research shows that TG gene of Pasundan cattle is monomorphic with C allele as the dominant allele (1.00). However, LEP gene of Pasundan cattle is polymorphic with C allele as the dominant allele (0.98) and T as the rare allele (0.02). The polymorphic informative content (PIC) and numberof effective allele (ne) values in the LEP gene in the animal studied were 0.04 and 1.04 respectively. It was concluded that TG/BstYI and LEP/Sau3AI gene in the present study can not be used as molecular selection in Pasundan cattle. These results are important as the basic information for preparing the molecular selection program in the future.
Journal of the Indonesian Tropical Animal Agriculture
Birth weight and calving interval are included of productivity traits that can be increased by se... more Birth weight and calving interval are included of productivity traits that can be increased by selection program. However, the standard of desirable birth weight in cattle during the selection program is important to prevent dystocia incident risk. This study was aimed to select Bali cattle (Bos javanicus) based on Estimated Breeding Value of birth weight (EBVBW) and Most Probable Producing Ability of birth weight (MPPABW) and calving interval (MPPACI). Total of 758 records data of BW were collected from Lombok and Sumbawa islands, West Nusa Tenggara Province. Research showed the average of BW in Bali calves were 15.69±1.70 kg (Lombok) and 13.49±1.89 (Sumbawa). The average of CI in Bali cows at both islands were about 385 days. In addition, the heritability (h2) values of BW in both islands was about 0.90. The repeatability (r) values of BW in both islands were about 0.30. Meanwhile, the r value of CI in Sumbawa island was 0.39. The highest of EBVBW for sire was +4.25 kg by bull’s I...
IOP Conference Series: Earth and Environmental Science
Biodiversitas Journal of Biological Diversity
Said S, Putra WPB. 2018. Short Communication: Novel Single Nucleotide Polymorphisms (SNPs) in the... more Said S, Putra WPB. 2018. Short Communication: Novel Single Nucleotide Polymorphisms (SNPs) in the 5'UTR of Bovine HeatShock Protein 70 (bHSP70) Gene and its association with Service per Conception (S/C) of Pasundan cattle. Biodiversitas 19: 1622-1625. Heat stress in the livestock reduces reproductive traits, including of service per conception (S/C). The influence of heat stress inlivestock can be handled through molecular selection. One of the candidate genes that is affecting heat stress tolerance is Bovine Heat ShockProtein 70 (bHSP70) gene. This research was carried out to detect the single nucleotide polymorphism (SNPs) in the 5'UTR of bHSP70 genefrom 44 heads of Pasundan cows at breeding station (BPPIBT-SP Ciamis, West Java). Research showed that 18 SNPs were detected withsequencing method. The insertion mutation was detected in all animal studied and occurred at position between g.1112 and g.1113. Themoderate of polymorphic informative content (PIC) were detected in t...
Jurnal Ilmu Ternak dan Veteriner
Susu sebagai sumber makanan utama bagi kehidupan mammalia memiliki kandungan elektrolit untuk men... more Susu sebagai sumber makanan utama bagi kehidupan mammalia memiliki kandungan elektrolit untuk menggantikan cairan tubuh yang hilang oleh karena aktivitas ataupun proses metabolisme. Konsentrasi total elektrolit dapat diukur berdasarkan nilai konduktivitas yang dihasilkan susu berdasarkan pada komposisi nutrisi yang dikandungnya. Dengan demikian hubungan parameter kualitas susu dengan nilai konduktifitas dapat menjadi tolok ukur untuk kualitas dan nilai jual susu sehingga lebih mudah diaplikasikan di lapangan. Penelitian ini bertujuan menganalisis hubungan antara electric conductivity (EC) dengan nilai kandungan susu sapi. Susu diambil dari 10-30 ekor sapi dari peternakan di Kabupaten Bandung Barat (Lembang),
Acta VETERINARIA Indonesiana
Q fever merupakan penyakit zoonosis yang disebabkan oleh bakteri gram negatif Coxiella burnetii d... more Q fever merupakan penyakit zoonosis yang disebabkan oleh bakteri gram negatif Coxiella burnetii dan terdistribusi luas di seluruh dunia. Gejala klinis pada hewan ternak bersifat tidak konsisten dan tidak spesifik. Metode imunohistokimia telah digunakan untuk mendiagnosis kasus Q fever pada hewan ternak. Antibodi yang digunakan untuk metode imunohistokimia masih tergolong mahal dan harus diimpor dari negara lain sehingga produksi antibodi menjadi peluang. Penelitian ini bertujuan untuk memproduksi antibodi poliklonal anti-C.burnetii dan mengkarakterisasi antibodi tersebut untuk mendeteksi antigen pada organ limpa, paru-paru, dan hati sapi dari Rumah Potong Hewan (RPH) di Medan yang sebelumnya telah dikonfirmasi positif C.burnetii. C. burnetii strain Nine Mile (NM) dan Complete Freund’s Adjuvant (CFA) digunakan untuk menginduksi antibodi pada dua ekor kelinci New Zealand White (NZW) jantan berumur 10-16 minggu. Boosting dilakukan menggunakan immunogen yang sama dengan Incomplete Freun...
Asian-Australasian journal of animal sciences, Jan 27, 2018
This research was conducted to study the genetic diversity in the several Indonesian cattle breed... more This research was conducted to study the genetic diversity in the several Indonesian cattle breeds using microsatellite markers as scientific evidence to classify the Indonesian cattle breeds. A total of 229 DNA samples consists of 10 cattle breeds were used in this study. The PCR process was conducted using 12 labeled primers. The size of allele was generated using the multiplex DNA fragment analysis. The POPGEN and CERVUS programs were used to obtain nA, ne, Ho, He, allele frequency, FIS, FIT, FST, Nm, HW, and PIC values. The MEGA program was used to generate a dendrogram that illustrate the relationship among cattle population. Bayesian clustering assignments were analyzed using STRUCTURE program. The GENETIX program was used to perform the correspondence factorial analysis (CFA). The GENALEX program was used to perform the principal coordinates analysis (PCoA) and AMOVA. The principal component analysis (PCA) was performed using adegenet package of R program. A total of 862 alle...
Indonesian Journal of Animal and Veterinary Sciences, 2007
Veterinary World, Jan 14, 2025
Background and Aim: Bali-polled bulls, known for their favorable traits, require advanced reprodu... more Background and Aim: Bali-polled bulls, known for their favorable traits, require advanced reproductive performance analysis to optimize breeding programs. This study aimed to evaluate sperm kinematics and seminal plasma proteomic profiles as biomarkers for sperm motility and fertility in Bali-polled bulls.
Materials and Methods: Semen from five Bali-polled bulls was collected biweekly over five batches using artificial vaginas. Sperm kinematics were assessed using computer-assisted semen analysis (CASA). Fertility was evaluated through service per conception (S/C) in artificial insemination trials. Seminal plasma proteins were analyzed through liquid chromatography–tandem mass spectrometry (LC-MS/MS) and annotated using the UniProt database, PANTHER for gene ontology, and STRING database for protein interactions.
Results: Post-thaw sperm kinematics showed satisfactory results, with a mean S/C of 1.52. Proteomic analysis identified 138 proteins, including six (TEX101, BSP1, PRSS55, BSP3, SPADH2, and TPPP2) linked to sperm motility. These proteins were involved in key biological processes such as sperm capacitation, motility regulation, and sperm-oocyte interaction.
Conclusion: Sperm kinematics and seminal plasma proteomics provide insights into Bali-polled bull fertility. Identified proteins can serve as fertility biomarkers, aiding in superior local breed development and reproductive efficiency improvement.
Keywords: artificial insemination, Bali-polled bulls, fertility biomarkers, seminal plasma proteomics, sperm kinematics.
Indian Journal of Animal Sciences, Jan 6, 2021
Livestock and Animal Research
Objective: The purpose of this research is to determine the effect of the rainy and dry seasons o... more Objective: The purpose of this research is to determine the effect of the rainy and dry seasons on the sperm qualities and frozen sperm production of Bali bulls at the Lelede Regional Artificial Insemination Center (RAIC) in West Nusa Tenggara.Methods: A total of 1226 ejaculates were examined from five Bali bulls during a 24-month period (November 2018–October 2019), which was classified into rainy (November–April, 658 ejaculates) and dry (May–October, 568 ejaculates) seasons. Semen volume (mL), sperm velocity (1-4), motility (%), concentration (x106 cell/ml), pre-freezing motility (%), post-freezing motility (%), recovery rate (%), and total frozen semen production were examined as sperm quality measures.Results: The results indicated that during the rainy season, ejaculates and processed sperm were more abundant than during the dry season. Additionally, the features of discarded fresh semen in the volume parameter were significantly (P<0.05) larger during the dry season than du...
Journal of the Indonesian Tropical Animal Agriculture, 2016
Telah dilakukan suatu penelitian untuk mengidentifikasi variasi gen myostatin dan mengevaluasi ke... more Telah dilakukan suatu penelitian untuk mengidentifikasi variasi gen myostatin dan mengevaluasi keberadaan marker genetik untuk sifat double muscling (delesi 11 basa pada ekson 3 gen myostatin) pada generasi pertama (F1) hasil persilangan dengan sapi Belgian Blue pertama di Indonesia. Sebanyak 8 sampel DNA sapi yang dipelihara di PT. Karya Anugerah Rumpin (KAR) digunakan dalam penelitian ini. Analisa Single Strand Conformation Polymorphism (SSCP) dan sekuensing digunakan untuk mengidentifikasi keragaman dari gen myostatin. Terdapat 3 tipe alel gen myostatin berdasarkan hasil analisa SSCP. Pejantan Belgian Blue memiliki tipe alel A. Sapi Simmental, Wagyu, SO x BX, Charolais, dan sapi PO memiliki tipe alel B. Sementara itu anak sapi hasil perkawinan Belgian Blue x FH dan Belgian Blue x SO memiliki tipe alel C (heterozigot). Berdasarkan hasil analisa sekuen gen myostatin, ditemukan adanya delesi 11 basa pada ekson 3 gen myostatin pejantan Belgian Blue. Hasil analisa sekuensing juga memberikan informasi bahwa generasi F1 (Belgian Blue x FH dan Belgian Blue x SO) memiliki gen myostatin yang berada dalam kondisi heterozigot. Hasil penelitian ini memberikan bukti ilmiah bahwa delesi 11 basa pada ekson 3 gen myostatin yang merupakan marker untuk sifat double muscling pada sapi Belgian Blue juga ada (diwariskan) pada anak-anak sapi generasi F1 (jantan dan betina).
Journal of the Indonesian Tropical Animal Agriculture, 2014
Penelitian ini bertujuan untuk menentukan angka kebuntingan dan kelahiran sapi Bali sesudah sinkr... more Penelitian ini bertujuan untuk menentukan angka kebuntingan dan kelahiran sapi Bali sesudah sinkronisasi estrus dan inseminasi buatan (IB) dengan semen beku sexing serta kesesuaian jenis kelamin anak. Penelitian dilakukan pada dua lokasi peternakan di Kabupaten Lombok Barat. Sinkronisasi estrus dilakukan melalui pemberian satu suntikan 5 cc Lutalyse ® yang mengandung 25 mg PGF 2α (Lutalyse, Sterile Solution, Pharmacia Animal Health) secara intra muscular. Pengamatan dilakukan pada sapi yang memberikan respon dan diinseminasi pada saat estrus. IB juga langsung dilakukan pada sapi-sapi yang menunjukkan birahi alami. Diagnosa kebuntingan dilakukan melalui palpasi rektal pada hari ke-90. Pada Peternakan 1, angka kebuntingan sapi-sapi yang mendapat perlakuan dan yang tidak mendapat perlakuan PGF 2α masing-masing sebesar 29/63 (46,03%) dan 71/75 (94,66%). Pada Peternakan 2, nilainilai yang bersesuaian masing-masing adalah 38/89 (42,70%) dan 10/23 (43,48%). Angka kelahiran anak pada kedua farm diperoleh 100%. Angka kesesuaian jenis kelamin untuk anak betina yang lahir di Peternakan 1 adalah 94.00%, sedangkan di Peternakan 2, untuk anak sapi yang terlahir jantan adalah 93.75%. Dibawah kondisi penelitian ini, sinkronisasi estrus yang diikuti dengan IB semen beku hasil sexing tampaknya dapat diaplikasikan di lapang sehingga menghasilkan angka kebuntingan dan kelahiran anak yang pantas diterima, serta angka kesesuaian di atas 90% untuk masing-masing jenis kelamin betina maupun jantan. Kata kunci: inseminasi buatan, sapi Bali, sinkronisasi estrus, semen beku-sexing, angka kesesuaian jenis kelamin
The presented study was aimed at determining the association of growth hormone (GH) gene polymorp... more The presented study was aimed at determining the association of growth hormone (GH) gene polymorphism with the traits that characterise carcass and meat quality and meat basic chemical composition in porkers with genotype CC with respect to RYR1 locus. The study was carried out on 126 PIC porkers originating after Camborough 22 sows and PIC 337 boars. The polymorphism of GH gene was identified respectively with restrictive enzymes MspI, HaeII, CfoI and ApaI. The allele frequencies were as follows: GH/MspI A = 0.78 and B = 0.22; GH/HaeII A = 0.86 and B = 0.14; GH/CfoI C1 = 0.09 and C2 = 0.03 and C3 = 0.18 and C4 = 0.70; GH/ApaI A1 = 0.28 and A2 = 0.72. The analysis of association of GH genotypes identified with four restrictive enzymes did not show any relationship between the GH gene polymorphism and the carcass slaughter traits determined with meatiness measuring apparatus CGM and the meat quality and meat basic chemical composition in PIC hybrid porkers. It may be stated, basing o...
APCBEE Procedia, 2014
Abstract The aim of this research was to determine the nutrient content, digestibility on wafer o... more Abstract The aim of this research was to determine the nutrient content, digestibility on wafer of feed supplement and productivity of Bali calves. This research had two steps experimental, the first steps experiment to determine the nutrient content and digestibility on wafer of feed supplement. The composition wafer of feed supplement i.e. T1 = wafer containing lamtoro leaf, T2 = wafer containing lamtoro and papaya leaf, T3 = wafer containing moringa leaf, T4 = wafer containing gamal leaf, T5= wafer containing corn leaf and corn. Nutrient content and digestibility of T1 had highest compared among the others, i.e. crude protein (32.34%), ash (7.24%), crude fiber (16.85%), crude fat (4.52%), NFE (39.06 cal/g), digestibility of dry matter 82.87%, digestibility of organic matter 81.78%, NH3 9.33% and VFA 164.55%. The second steps of this research was the best result of the nutrient content and digestibility of wafer of feed supplements tested to bali calves. Level of wafer containing lamtoro leaf,i.e R1 =0%, R2 =5%, R3 =10%, R4=15%. The result indicated that treatments had significant effect (P
Journal of the Indonesian Tropical Animal Agriculture, 2016
Selection of livestock was purposed to increase productivity. A performance test was conducted us... more Selection of livestock was purposed to increase productivity. A performance test was conducted using 25 males and 25 females of Sumba Ongole (SO) cattle. The performance test were conducted in three periods (2014, 2015 and 2016) for about 535 days. Descriptive statistics ws used to analyze data of body weight. Cattle with age between 300 to 600 days were used for evaluation. Result showed that heritability value of yearling weight (YW365) was 0.77+0.68 as a high category. The highest breeding value of YW365 were 66.05 kg (male) and 41.89 kg (female). The average of corrected final weight (CFW) weight were 172.55+34.22 kg (male) and 159.80+37.73 kg (female). The performance test in this study obtained six A class (1 bull and 5 cows) based on the standard minimum of body measurements for SO cattle.
Veterinary World
Background and Aim: Capacity for sperm production is affected by age, which is related to the mor... more Background and Aim: Capacity for sperm production is affected by age, which is related to the morphology of sperm abnormalities and can affect fertility. The aim of this study was to evaluate the relationship between age and concentrations of testosterone and adiponectin with sperm abnormalities in Simmental bulls. Materials and Methods: The study used 11 bulls, separated into three groups. The first group consisted of five bulls aged 4-5 years, and the second and third groups each consisted of three bulls, aged 6-7 and 8-10 years, respectively. The average sperm motility of the animals ranged from 57.66±2.60% to 70.17±0.22%. Blood samples were obtained from the coccygeal region of the animals. Testosterone and adiponectin analysis was performed using the enzyme-linked immunosorbent assay method. Sperm morphology was evaluated using carbol fuchsin-eosin staining according to the Williams method. Finally, correlations between testosterone and adiponectin concentrations, age, and sper...
Journal of the Indonesian Tropical Animal Agriculture
The objective of the current study was to investigate the effect of addition an amino acid or its... more The objective of the current study was to investigate the effect of addition an amino acid or its combination with EDTA on DNA integrity and morphometry sperm heads of freeze-dried bovine spermatozoa. Spermatozoa were freeze-dried in medium with the addition of an amino acid glycine, cysteine, glutamine, or its combination with EDTA. Sperm head morphometry was identified at 400X magnification using Axio Vision(Zeiss Company, Germany), while for membrane plasma integrity (MPI) determined by calculation of the percentage of spermatozoa having intact plasma membrane by osmotic resistance test method and DNA integrity analysis using acridine orange staining. Sperm head had declined in size after the freeze-drying process, MPI of FD spermatozoa gradually increased significantly when FD solution was added with an amino acid solution (glycine, cysteine) and its combination with EDTA. DNA integrity of all freeze-dried spermatozoa treatments was remaining intact, no significantly different (...
Buletin Peternakan
The Thyroglobulin (TG) and Leptin (LEP) genes are two candidate genes that widely used for molecu... more The Thyroglobulin (TG) and Leptin (LEP) genes are two candidate genes that widely used for molecular selection to improve carcass traits in beef cattle. This research was carried out to identify the genetic characterization of TG and LEP genes from 47 heads of Pasundan cows at West Java using PCR-RFLP method. Research shows that TG gene of Pasundan cattle is monomorphic with C allele as the dominant allele (1.00). However, LEP gene of Pasundan cattle is polymorphic with C allele as the dominant allele (0.98) and T as the rare allele (0.02). The polymorphic informative content (PIC) and numberof effective allele (ne) values in the LEP gene in the animal studied were 0.04 and 1.04 respectively. It was concluded that TG/BstYI and LEP/Sau3AI gene in the present study can not be used as molecular selection in Pasundan cattle. These results are important as the basic information for preparing the molecular selection program in the future.
Journal of the Indonesian Tropical Animal Agriculture
Birth weight and calving interval are included of productivity traits that can be increased by se... more Birth weight and calving interval are included of productivity traits that can be increased by selection program. However, the standard of desirable birth weight in cattle during the selection program is important to prevent dystocia incident risk. This study was aimed to select Bali cattle (Bos javanicus) based on Estimated Breeding Value of birth weight (EBVBW) and Most Probable Producing Ability of birth weight (MPPABW) and calving interval (MPPACI). Total of 758 records data of BW were collected from Lombok and Sumbawa islands, West Nusa Tenggara Province. Research showed the average of BW in Bali calves were 15.69±1.70 kg (Lombok) and 13.49±1.89 (Sumbawa). The average of CI in Bali cows at both islands were about 385 days. In addition, the heritability (h2) values of BW in both islands was about 0.90. The repeatability (r) values of BW in both islands were about 0.30. Meanwhile, the r value of CI in Sumbawa island was 0.39. The highest of EBVBW for sire was +4.25 kg by bull’s I...
IOP Conference Series: Earth and Environmental Science
Biodiversitas Journal of Biological Diversity
Said S, Putra WPB. 2018. Short Communication: Novel Single Nucleotide Polymorphisms (SNPs) in the... more Said S, Putra WPB. 2018. Short Communication: Novel Single Nucleotide Polymorphisms (SNPs) in the 5'UTR of Bovine HeatShock Protein 70 (bHSP70) Gene and its association with Service per Conception (S/C) of Pasundan cattle. Biodiversitas 19: 1622-1625. Heat stress in the livestock reduces reproductive traits, including of service per conception (S/C). The influence of heat stress inlivestock can be handled through molecular selection. One of the candidate genes that is affecting heat stress tolerance is Bovine Heat ShockProtein 70 (bHSP70) gene. This research was carried out to detect the single nucleotide polymorphism (SNPs) in the 5'UTR of bHSP70 genefrom 44 heads of Pasundan cows at breeding station (BPPIBT-SP Ciamis, West Java). Research showed that 18 SNPs were detected withsequencing method. The insertion mutation was detected in all animal studied and occurred at position between g.1112 and g.1113. Themoderate of polymorphic informative content (PIC) were detected in t...
Jurnal Ilmu Ternak dan Veteriner
Susu sebagai sumber makanan utama bagi kehidupan mammalia memiliki kandungan elektrolit untuk men... more Susu sebagai sumber makanan utama bagi kehidupan mammalia memiliki kandungan elektrolit untuk menggantikan cairan tubuh yang hilang oleh karena aktivitas ataupun proses metabolisme. Konsentrasi total elektrolit dapat diukur berdasarkan nilai konduktivitas yang dihasilkan susu berdasarkan pada komposisi nutrisi yang dikandungnya. Dengan demikian hubungan parameter kualitas susu dengan nilai konduktifitas dapat menjadi tolok ukur untuk kualitas dan nilai jual susu sehingga lebih mudah diaplikasikan di lapangan. Penelitian ini bertujuan menganalisis hubungan antara electric conductivity (EC) dengan nilai kandungan susu sapi. Susu diambil dari 10-30 ekor sapi dari peternakan di Kabupaten Bandung Barat (Lembang),
Acta VETERINARIA Indonesiana
Q fever merupakan penyakit zoonosis yang disebabkan oleh bakteri gram negatif Coxiella burnetii d... more Q fever merupakan penyakit zoonosis yang disebabkan oleh bakteri gram negatif Coxiella burnetii dan terdistribusi luas di seluruh dunia. Gejala klinis pada hewan ternak bersifat tidak konsisten dan tidak spesifik. Metode imunohistokimia telah digunakan untuk mendiagnosis kasus Q fever pada hewan ternak. Antibodi yang digunakan untuk metode imunohistokimia masih tergolong mahal dan harus diimpor dari negara lain sehingga produksi antibodi menjadi peluang. Penelitian ini bertujuan untuk memproduksi antibodi poliklonal anti-C.burnetii dan mengkarakterisasi antibodi tersebut untuk mendeteksi antigen pada organ limpa, paru-paru, dan hati sapi dari Rumah Potong Hewan (RPH) di Medan yang sebelumnya telah dikonfirmasi positif C.burnetii. C. burnetii strain Nine Mile (NM) dan Complete Freund’s Adjuvant (CFA) digunakan untuk menginduksi antibodi pada dua ekor kelinci New Zealand White (NZW) jantan berumur 10-16 minggu. Boosting dilakukan menggunakan immunogen yang sama dengan Incomplete Freun...
Asian-Australasian journal of animal sciences, Jan 27, 2018
This research was conducted to study the genetic diversity in the several Indonesian cattle breed... more This research was conducted to study the genetic diversity in the several Indonesian cattle breeds using microsatellite markers as scientific evidence to classify the Indonesian cattle breeds. A total of 229 DNA samples consists of 10 cattle breeds were used in this study. The PCR process was conducted using 12 labeled primers. The size of allele was generated using the multiplex DNA fragment analysis. The POPGEN and CERVUS programs were used to obtain nA, ne, Ho, He, allele frequency, FIS, FIT, FST, Nm, HW, and PIC values. The MEGA program was used to generate a dendrogram that illustrate the relationship among cattle population. Bayesian clustering assignments were analyzed using STRUCTURE program. The GENETIX program was used to perform the correspondence factorial analysis (CFA). The GENALEX program was used to perform the principal coordinates analysis (PCoA) and AMOVA. The principal component analysis (PCA) was performed using adegenet package of R program. A total of 862 alle...
Indonesian Journal of Animal and Veterinary Sciences, 2007