TENG HUA - Academia.edu (original) (raw)
Papers by TENG HUA
Tangniaobing Xinshijie, 2019
Electronics, 2022
The Global Navigation Satellite System (GNSS) becomes vulnerable in a challenging environment, am... more The Global Navigation Satellite System (GNSS) becomes vulnerable in a challenging environment, among which spoofing is the most dangerous threat. Meaconing, as the most convenient way to conduct spoofing, is widely studied around the world, and also leads to lots of research into corresponding anti-spoofing techniques. This paper develops a semi-hardware meaconing platform and proposes a novel GPS meaconing spoofing detection method based on Improved Ratio combined with Carrier-to-noise Moving variance (C/N0 – MV). The effectiveness has been validated theoretically and experimentally. The proposed method is proven useful when the meaconing signal has 5 dB power gain over the authentic signal, presenting 98% detection rate whereas the classic Signal quality monitoring (SQM) method with the Ratio metric presents only 30%.
Remote Sensing, 2021
Ionospheric scintillation frequently occurs in equatorial, auroral and polar regions, posing a th... more Ionospheric scintillation frequently occurs in equatorial, auroral and polar regions, posing a threat to the performance of the global navigation satellite system (GNSS). Thus, the detection of ionospheric scintillation is of great significance in regard to improving GNSS performance, especially when severe ionospheric scintillation occurs. Normal algorithms exhibit insensitivity in strong scintillation detection in that the natural phenomenon of strong scintillation appears only occasionally, and such samples account for a small proportion of the data in datasets relative to those for weak/moderate scintillation events. Aiming at improving the detection accuracy, we proposed a strategy combining an improved eXtreme Gradient Boosting (XGBoost) algorithm by using the synthetic minority, oversampling technique and edited nearest neighbor (SMOTE-ENN) resampling technique for detecting events imbalanced with respect to weak, medium and strong ionospheric scintillation. It outperformed t...
Journal of China West Normal University, 2007
This paper presents a standardized natural gradient ICA(Independent Component Analysis) algorithm... more This paper presents a standardized natural gradient ICA(Independent Component Analysis) algorithm through improving the natural gradient ICA algorithm based on Lie Group Invariance.The new algorithm introduces a standardizing factor which makes the absolute value of the determinant of parameter matrix equal to one.Therefore,the learning process is more stable and faster by restricting the drastic change of parameter matrix.In addition,the new algorithm is simpler by using standardizing factor to simplify the general criterion function.In BSS(Blind Signal Separation) simulation experiment,we have compared three algorithms including the general gradient ICA algorithm,the natural gradient ICA algorithm and the new algorithm.The results show the third is the best in the precision of restoring signals and converges fastest.
Commercial Research, 2009
This paper presents a measurement tool which is composed of PCA and SVM to evaluate the competiti... more This paper presents a measurement tool which is composed of PCA and SVM to evaluate the competitiveness for top managers of commercial banks,based on the definition of competitiveness evaluation index system for top managers of commercial banks.The initial data are standardized.Then the redundancy and relativity among the indexes are eliminated by making use of PCA.Finally,the competitiveness of top managers of commercial banks is evaluated by means of SVM under the environment of MATLAB 6.5.The results show that the method is an effective method of competitiveness evaluation for top managers of commercial banks.
A country profile based on LADM has been conceptualised in the last few years as part of joint re... more A country profile based on LADM has been conceptualised in the last few years as part of joint research efforts between the Department of Survey and Mapping Malaysia (JUPEM) and Universiti Teknologi Malaysia (UTM). The LADM conceptual model covers various aspects of spatial data components available in the mapping agency (JUPEM), including data from Land Office e.g. land registration. The Malaysian LADM country profile covers various classes of spatial unit including strata objects classes such as MY_Building, MY_ParcelUnit, MY_AccessoryUnit, MY_CommonPropertyUnit, MY_LimitedCommonPropertyUnit and MY_LandParcel. This paper discusses one experiment of converting the available building strata schema into LADM model, specifically MY_SpatialUnit for 2D and 3D. The existing building strata schema was developed based on XML syntax. The entire workflow of the experiment will be demonstrated where conversion of file with strata XML data collected from the field into the database with LADM d...
International journal of clinical and experimental pathology, 2017
Tumor microenvironment plays a critical role in cancer pathogenesis. In this study, we performed ... more Tumor microenvironment plays a critical role in cancer pathogenesis. In this study, we performed transcriptomic analysis of stromal cells from patients diagnosed with endometrial carcinoma. Endometrial stromal cells from patients and healthy donors were cultured and total RNA was extracted for RNA integrity examination and gene profiling analysis. Gene ontology (GO) and KEGG analysis were also performed. In this study, we found that, in endometrial stromal cells from endometrial cancer patients, a total of 605 genes were changed (fold change ≥2, p-value <0.05). From these, 275 were up-regulated and 330 were down-regulated genes. In addition, GO analysis showed that the differentially expressed genes (DEGs) were involved in various biological processes including cell adhesion, biological adhesion, bone development and extracellular matrix organization. Furthermore, KEGG analysis of the DEGs identified four pathways including Wnt signaling pathway, cadherin signaling pathway, ECM-r...
Journal of Practical Medical Techniques, 2006
Summary As the leading agency in Malaysia on Cadastral Survey, the Department of Survey and Mappi... more Summary As the leading agency in Malaysia on Cadastral Survey, the Department of Survey and Mapping Malaysia (JUPEM) is responsible to further modernize the cadastral system in Peninsular Malaysia. Changes have been made technically, operationally, structurally and institutionally in Malaysia’s cadastral survey system from time to time to ensure the Department’s relevancy in serving the society. The motivations for these changes are mainly due to the requirement for increased service provision and efficiency, and the larger needs of clients and governments. As public expectation relating to land delivery system increases, the need to move from a single purpose cadastre (its main focus is on the issuance of Titles) to a multipurpose cadastre (MPC) environment seemed significant to meet the demands. Thus, in its bid to understand the MPC concept and its implementation, a pilot study was conducted by the Department under the 10 th Malaysian Development Plan at the Federal Territory (FT...
Journal of Shanghai University of Engineering Science, 2009
The engine running-in testing data were processed based on the cubic spline method and least-squa... more The engine running-in testing data were processed based on the cubic spline method and least-square piecewise polynomial fitting method according to different situations in the Matlab environment,and precise curves were obtained which lay a sound foundation for further engine research.
There is no doubt that the cadastral map provides a useful entrance to information in a land parc... more There is no doubt that the cadastral map provides a useful entrance to information in a land parcel based information system. However, such information system could be made more meaningful and useful if it can be extended for multiple usages with multi data layers, and in three-dimensions (3D). Currently, many national mapping and cadastral agencies (NMCAs) and users deal with complex situations, and we believe that 3D could enhance the understanding of the situations better. This paper describes the new initiative of Malaysian NMCA towards developing a multipurpose cadastre (MPC) based on large scale spatial data layers grouped into two types of sub systems. The first group consists of components with spatial objects to which legal and administrative facts are attached (rights, restrictions and responsibilities), such as 3D cadastre (volume parcels), 3D marine cadastre, and 3D strata (in buildings). The second group consists of components with reference spatial objects, such as: 3D...
IET Radar, Sonar & Navigation, 2021
Journal of Medical Imaging and Health Informatics, 2021
Objective: To explore the evaluation indicators of X-ray radiographs for femoroacetabular impinge... more Objective: To explore the evaluation indicators of X-ray radiographs for femoroacetabular impingement (FAI) syndrome, and to propose therapeutic plans for FAI syndrome by using X-ray radiographs. Methods: From June 2016 to May 2018, patients who were diagnosed as FAI syndrome by hip joint anteroposterior radiographs in Second Yinzhou District Hospital were selected as research objects. After screening, the surgical treatment group included 52 cases, totally. Another 30 healthy adults who received X-ray radiography were included as the control group. Each included patient underwent minimally invasive surgery under hip arthroscopy. The efficacy indicators were evaluated, and the data were statistically analyzed. Results: By analyzing the basic data of the subjects, it was found that there was no significant difference in gender and body mass index between the healthy people and FAI patients (P > 0.05), but the VAS and Harris scores of 7d, 30d, 90d, and 180d after surgery were signi...
OncoTargets and Therapy, 2019
Background: Activation of NLPR3 inflammasome is associated with the development and progression o... more Background: Activation of NLPR3 inflammasome is associated with the development and progression of some types of malignant tumors, but its role in endometrial cancer is unclear. This study aimed to investigate the expression and function of NLRP3 inflammasome in endometrial cancer. Materials and methods: The expression levels of NLRP3, its inflammasome components and estrogen receptor β in endometrial cancer and paired non-tumor tissues were detected. The effects of NLPR3 silencing or overexpression on the proliferation, migration, and invasion of Ishikawa and HEC-1A cells were determined. The impact of NLPR3 silencing on the growth of implanted tumors was determined in vivo. The effects of estrogen on NLPR3 inflammasome activation and Ishikawa cell proliferation were determined. Results: The upregulation of NLRP3, ASC, caspase-1, and IL-1β was associated with the progression of endometrial cancer and poor survival. NLPR3 silencing inhibited the proliferation, migration, and invasion of endometrial cancer cells while NLPR3 overexpression had opposite effects. NLPR3 silencing reduced IL-1β and caspase-1 expression and the growth of implanted endometrial tumors, accompanied by decreased pro-IL-1β maturation. Estrogen enhanced NLPR3, ERβ, pro-IL-1β, IL-1β expression, and endometrial cancer cell proliferation, which were mitigated by treatment with ERβ inhibitor but not ERα inhibitor. Conclusion: Our results suggest that estrogen acts through ERβ to enhance the activation of NLPR3 inflammasome and promote the progression of endometrial cancer. NLPR3 inflammasome may be a new therapeutic target for endometrial cancer.
Cellular Physiology and Biochemistry, 2018
Background/Aims: This study is aimed at identification of miR-195-5p/MMP14 expression in cervical... more Background/Aims: This study is aimed at identification of miR-195-5p/MMP14 expression in cervical cancer (CC) and their roles on cell proliferation and invasion profile of CC cells through TNF signaling pathway in CC. Methods: Microarray analysis, gene set enrichment analysis (GSEA) and DAVID were used to analyze differentially expressed miRNAs, mRNAs and signaling pathways. MiR-195-5p and MMP14 expression levels in CC cell were determined by qRT-PCR. Western blot was employed to measure MMP14 and TNF signaling pathway-relating protein level. Luciferase reporter system was used to confirm the targeting relationship between MMP14 and miR-195-5p. Cell proliferation and invasion was respectively deeded by CCK8, transwell. In vivo experiment was carried out to study the impact of MMP14 and miR-195-5p on CC development in mice. Results: The microarray analysis and the results of qRT-PCR determined that miR-195-5p was under-expressed and MMP14 was over-expressed in CC cells. GSEA and DAVI...
Oncology Letters, 2018
Endometrial cancer (EC) is one of the most common gynecological cancer types worldwide. However, ... more Endometrial cancer (EC) is one of the most common gynecological cancer types worldwide. However, to the best of our knowledge, its underlying mechanisms remain unknown. The current study downloaded three mRNA and microRNA (miRNA) datasets of EC and normal tissue samples, GSE17025, GSE63678 and GSE35794, from the Gene Expression Omnibus to identify differentially expressed genes (DEGs) and miRNAs (DEMs) in EC tumor tissues. The DEGs and DEMs were then validated using data from The Cancer Genome Atlas and subjected to gene ontology and Kyoto Encyclopedia of Genes and Genomes pathway analysis. STRING and Cytoscape were used to construct a protein-protein interaction network and the prognostic effects of the hub genes were analyzed. Finally, miRecords was used to predict DEM targets and an miRNA-gene network was constructed. A total of 160 DEGs were identified, of which 51 genes were highly expressed and 100 DEGs were discovered from the PPI network. Three overlapping genes between the DEGs and the DEM targets, BIRC5, CENPF and HJURP, were associated with significantly worse overall survival of patients with EC. A number of DEGs were enriched in cell cycle, human T-lymphotropic virus infection and cancer-associated pathways. A total of 20 DEMs and 29 miRNA gene pairs were identified. In conclusion, the identified DEGs, DEMs and pathways in EC may provide new insights into understanding the underlying molecular mechanisms that facilitate EC tumorigenesis and progression.
Oncology reports, Jan 4, 2018
S100 calcium binding protein A4 (S100A4) is a well‑established tumor metastasis mediator in vario... more S100 calcium binding protein A4 (S100A4) is a well‑established tumor metastasis mediator in various malignancies, including endometrial cancer (EC). However, the regulatory mechanism underlying S100A4 expression remains elusive. In the present study, by analyzing public datasets and clinical samples, we found that estrogen‑related receptor γ (ERRγ) was upregulated and positively correlated with S100A4 transcription in EC. ERRγ knockdown inhibited S100A4 expression and promoted the expression of its downstream target E‑cadherin, and vice versa. Mechanistic studies indicated that ERRγ enhanced the promoter activity of S100A4 to facilitate its transcription. In addition, knockdown of ERRγ suppressed migration and invasion of EC cells in vitro, while ectopic ERRγ expression promoted migration and invasion of EC cells in vitro and tumor growth in vivo. Importantly, restoration of S100A4 expression prevented EC cells from undergoing ERRγ‑mediated changes in these biological features. In a...
Scientific reports, Jan 18, 2018
Studies have shown that calcium sensing receptor (CaSR) is involved in the progressions of severa... more Studies have shown that calcium sensing receptor (CaSR) is involved in the progressions of several human cancers. However, the role of CaSR in endometrial cancer remains unknown. This study provides a preliminary analysis of the CaSR effect on endometrial cancer development. Ectopic CaSR expression by lentiviral transfection (CaSR-OV) in Ishikawa cells significantly increased intracellular calcium ([Ca]) levels and cell apoptosis. E-cadherin and β-catenin expression and complex formation at the membrane were increased in CaSR-OV Ishikawa cells relative to control Ishikawa cells (vector). Furthermore, CaSR-OV Ishikawa cells showed a reduced invasive potential, which was attributed to E-cadherin/β-catenin complex formation. Moreover, a reduction in CaSR expression in endometrial cancer relative to normal specimens was evident by immunohistochemistry and was positively associated with E-cadherin, but not β-catenin, expression. Furthermore, VEGFR3 was significantly down-regulated in CaS...
Tangniaobing Xinshijie, 2019
Electronics, 2022
The Global Navigation Satellite System (GNSS) becomes vulnerable in a challenging environment, am... more The Global Navigation Satellite System (GNSS) becomes vulnerable in a challenging environment, among which spoofing is the most dangerous threat. Meaconing, as the most convenient way to conduct spoofing, is widely studied around the world, and also leads to lots of research into corresponding anti-spoofing techniques. This paper develops a semi-hardware meaconing platform and proposes a novel GPS meaconing spoofing detection method based on Improved Ratio combined with Carrier-to-noise Moving variance (C/N0 – MV). The effectiveness has been validated theoretically and experimentally. The proposed method is proven useful when the meaconing signal has 5 dB power gain over the authentic signal, presenting 98% detection rate whereas the classic Signal quality monitoring (SQM) method with the Ratio metric presents only 30%.
Remote Sensing, 2021
Ionospheric scintillation frequently occurs in equatorial, auroral and polar regions, posing a th... more Ionospheric scintillation frequently occurs in equatorial, auroral and polar regions, posing a threat to the performance of the global navigation satellite system (GNSS). Thus, the detection of ionospheric scintillation is of great significance in regard to improving GNSS performance, especially when severe ionospheric scintillation occurs. Normal algorithms exhibit insensitivity in strong scintillation detection in that the natural phenomenon of strong scintillation appears only occasionally, and such samples account for a small proportion of the data in datasets relative to those for weak/moderate scintillation events. Aiming at improving the detection accuracy, we proposed a strategy combining an improved eXtreme Gradient Boosting (XGBoost) algorithm by using the synthetic minority, oversampling technique and edited nearest neighbor (SMOTE-ENN) resampling technique for detecting events imbalanced with respect to weak, medium and strong ionospheric scintillation. It outperformed t...
Journal of China West Normal University, 2007
This paper presents a standardized natural gradient ICA(Independent Component Analysis) algorithm... more This paper presents a standardized natural gradient ICA(Independent Component Analysis) algorithm through improving the natural gradient ICA algorithm based on Lie Group Invariance.The new algorithm introduces a standardizing factor which makes the absolute value of the determinant of parameter matrix equal to one.Therefore,the learning process is more stable and faster by restricting the drastic change of parameter matrix.In addition,the new algorithm is simpler by using standardizing factor to simplify the general criterion function.In BSS(Blind Signal Separation) simulation experiment,we have compared three algorithms including the general gradient ICA algorithm,the natural gradient ICA algorithm and the new algorithm.The results show the third is the best in the precision of restoring signals and converges fastest.
Commercial Research, 2009
This paper presents a measurement tool which is composed of PCA and SVM to evaluate the competiti... more This paper presents a measurement tool which is composed of PCA and SVM to evaluate the competitiveness for top managers of commercial banks,based on the definition of competitiveness evaluation index system for top managers of commercial banks.The initial data are standardized.Then the redundancy and relativity among the indexes are eliminated by making use of PCA.Finally,the competitiveness of top managers of commercial banks is evaluated by means of SVM under the environment of MATLAB 6.5.The results show that the method is an effective method of competitiveness evaluation for top managers of commercial banks.
A country profile based on LADM has been conceptualised in the last few years as part of joint re... more A country profile based on LADM has been conceptualised in the last few years as part of joint research efforts between the Department of Survey and Mapping Malaysia (JUPEM) and Universiti Teknologi Malaysia (UTM). The LADM conceptual model covers various aspects of spatial data components available in the mapping agency (JUPEM), including data from Land Office e.g. land registration. The Malaysian LADM country profile covers various classes of spatial unit including strata objects classes such as MY_Building, MY_ParcelUnit, MY_AccessoryUnit, MY_CommonPropertyUnit, MY_LimitedCommonPropertyUnit and MY_LandParcel. This paper discusses one experiment of converting the available building strata schema into LADM model, specifically MY_SpatialUnit for 2D and 3D. The existing building strata schema was developed based on XML syntax. The entire workflow of the experiment will be demonstrated where conversion of file with strata XML data collected from the field into the database with LADM d...
International journal of clinical and experimental pathology, 2017
Tumor microenvironment plays a critical role in cancer pathogenesis. In this study, we performed ... more Tumor microenvironment plays a critical role in cancer pathogenesis. In this study, we performed transcriptomic analysis of stromal cells from patients diagnosed with endometrial carcinoma. Endometrial stromal cells from patients and healthy donors were cultured and total RNA was extracted for RNA integrity examination and gene profiling analysis. Gene ontology (GO) and KEGG analysis were also performed. In this study, we found that, in endometrial stromal cells from endometrial cancer patients, a total of 605 genes were changed (fold change ≥2, p-value <0.05). From these, 275 were up-regulated and 330 were down-regulated genes. In addition, GO analysis showed that the differentially expressed genes (DEGs) were involved in various biological processes including cell adhesion, biological adhesion, bone development and extracellular matrix organization. Furthermore, KEGG analysis of the DEGs identified four pathways including Wnt signaling pathway, cadherin signaling pathway, ECM-r...
Journal of Practical Medical Techniques, 2006
Summary As the leading agency in Malaysia on Cadastral Survey, the Department of Survey and Mappi... more Summary As the leading agency in Malaysia on Cadastral Survey, the Department of Survey and Mapping Malaysia (JUPEM) is responsible to further modernize the cadastral system in Peninsular Malaysia. Changes have been made technically, operationally, structurally and institutionally in Malaysia’s cadastral survey system from time to time to ensure the Department’s relevancy in serving the society. The motivations for these changes are mainly due to the requirement for increased service provision and efficiency, and the larger needs of clients and governments. As public expectation relating to land delivery system increases, the need to move from a single purpose cadastre (its main focus is on the issuance of Titles) to a multipurpose cadastre (MPC) environment seemed significant to meet the demands. Thus, in its bid to understand the MPC concept and its implementation, a pilot study was conducted by the Department under the 10 th Malaysian Development Plan at the Federal Territory (FT...
Journal of Shanghai University of Engineering Science, 2009
The engine running-in testing data were processed based on the cubic spline method and least-squa... more The engine running-in testing data were processed based on the cubic spline method and least-square piecewise polynomial fitting method according to different situations in the Matlab environment,and precise curves were obtained which lay a sound foundation for further engine research.
There is no doubt that the cadastral map provides a useful entrance to information in a land parc... more There is no doubt that the cadastral map provides a useful entrance to information in a land parcel based information system. However, such information system could be made more meaningful and useful if it can be extended for multiple usages with multi data layers, and in three-dimensions (3D). Currently, many national mapping and cadastral agencies (NMCAs) and users deal with complex situations, and we believe that 3D could enhance the understanding of the situations better. This paper describes the new initiative of Malaysian NMCA towards developing a multipurpose cadastre (MPC) based on large scale spatial data layers grouped into two types of sub systems. The first group consists of components with spatial objects to which legal and administrative facts are attached (rights, restrictions and responsibilities), such as 3D cadastre (volume parcels), 3D marine cadastre, and 3D strata (in buildings). The second group consists of components with reference spatial objects, such as: 3D...
IET Radar, Sonar & Navigation, 2021
Journal of Medical Imaging and Health Informatics, 2021
Objective: To explore the evaluation indicators of X-ray radiographs for femoroacetabular impinge... more Objective: To explore the evaluation indicators of X-ray radiographs for femoroacetabular impingement (FAI) syndrome, and to propose therapeutic plans for FAI syndrome by using X-ray radiographs. Methods: From June 2016 to May 2018, patients who were diagnosed as FAI syndrome by hip joint anteroposterior radiographs in Second Yinzhou District Hospital were selected as research objects. After screening, the surgical treatment group included 52 cases, totally. Another 30 healthy adults who received X-ray radiography were included as the control group. Each included patient underwent minimally invasive surgery under hip arthroscopy. The efficacy indicators were evaluated, and the data were statistically analyzed. Results: By analyzing the basic data of the subjects, it was found that there was no significant difference in gender and body mass index between the healthy people and FAI patients (P > 0.05), but the VAS and Harris scores of 7d, 30d, 90d, and 180d after surgery were signi...
OncoTargets and Therapy, 2019
Background: Activation of NLPR3 inflammasome is associated with the development and progression o... more Background: Activation of NLPR3 inflammasome is associated with the development and progression of some types of malignant tumors, but its role in endometrial cancer is unclear. This study aimed to investigate the expression and function of NLRP3 inflammasome in endometrial cancer. Materials and methods: The expression levels of NLRP3, its inflammasome components and estrogen receptor β in endometrial cancer and paired non-tumor tissues were detected. The effects of NLPR3 silencing or overexpression on the proliferation, migration, and invasion of Ishikawa and HEC-1A cells were determined. The impact of NLPR3 silencing on the growth of implanted tumors was determined in vivo. The effects of estrogen on NLPR3 inflammasome activation and Ishikawa cell proliferation were determined. Results: The upregulation of NLRP3, ASC, caspase-1, and IL-1β was associated with the progression of endometrial cancer and poor survival. NLPR3 silencing inhibited the proliferation, migration, and invasion of endometrial cancer cells while NLPR3 overexpression had opposite effects. NLPR3 silencing reduced IL-1β and caspase-1 expression and the growth of implanted endometrial tumors, accompanied by decreased pro-IL-1β maturation. Estrogen enhanced NLPR3, ERβ, pro-IL-1β, IL-1β expression, and endometrial cancer cell proliferation, which were mitigated by treatment with ERβ inhibitor but not ERα inhibitor. Conclusion: Our results suggest that estrogen acts through ERβ to enhance the activation of NLPR3 inflammasome and promote the progression of endometrial cancer. NLPR3 inflammasome may be a new therapeutic target for endometrial cancer.
Cellular Physiology and Biochemistry, 2018
Background/Aims: This study is aimed at identification of miR-195-5p/MMP14 expression in cervical... more Background/Aims: This study is aimed at identification of miR-195-5p/MMP14 expression in cervical cancer (CC) and their roles on cell proliferation and invasion profile of CC cells through TNF signaling pathway in CC. Methods: Microarray analysis, gene set enrichment analysis (GSEA) and DAVID were used to analyze differentially expressed miRNAs, mRNAs and signaling pathways. MiR-195-5p and MMP14 expression levels in CC cell were determined by qRT-PCR. Western blot was employed to measure MMP14 and TNF signaling pathway-relating protein level. Luciferase reporter system was used to confirm the targeting relationship between MMP14 and miR-195-5p. Cell proliferation and invasion was respectively deeded by CCK8, transwell. In vivo experiment was carried out to study the impact of MMP14 and miR-195-5p on CC development in mice. Results: The microarray analysis and the results of qRT-PCR determined that miR-195-5p was under-expressed and MMP14 was over-expressed in CC cells. GSEA and DAVI...
Oncology Letters, 2018
Endometrial cancer (EC) is one of the most common gynecological cancer types worldwide. However, ... more Endometrial cancer (EC) is one of the most common gynecological cancer types worldwide. However, to the best of our knowledge, its underlying mechanisms remain unknown. The current study downloaded three mRNA and microRNA (miRNA) datasets of EC and normal tissue samples, GSE17025, GSE63678 and GSE35794, from the Gene Expression Omnibus to identify differentially expressed genes (DEGs) and miRNAs (DEMs) in EC tumor tissues. The DEGs and DEMs were then validated using data from The Cancer Genome Atlas and subjected to gene ontology and Kyoto Encyclopedia of Genes and Genomes pathway analysis. STRING and Cytoscape were used to construct a protein-protein interaction network and the prognostic effects of the hub genes were analyzed. Finally, miRecords was used to predict DEM targets and an miRNA-gene network was constructed. A total of 160 DEGs were identified, of which 51 genes were highly expressed and 100 DEGs were discovered from the PPI network. Three overlapping genes between the DEGs and the DEM targets, BIRC5, CENPF and HJURP, were associated with significantly worse overall survival of patients with EC. A number of DEGs were enriched in cell cycle, human T-lymphotropic virus infection and cancer-associated pathways. A total of 20 DEMs and 29 miRNA gene pairs were identified. In conclusion, the identified DEGs, DEMs and pathways in EC may provide new insights into understanding the underlying molecular mechanisms that facilitate EC tumorigenesis and progression.
Oncology reports, Jan 4, 2018
S100 calcium binding protein A4 (S100A4) is a well‑established tumor metastasis mediator in vario... more S100 calcium binding protein A4 (S100A4) is a well‑established tumor metastasis mediator in various malignancies, including endometrial cancer (EC). However, the regulatory mechanism underlying S100A4 expression remains elusive. In the present study, by analyzing public datasets and clinical samples, we found that estrogen‑related receptor γ (ERRγ) was upregulated and positively correlated with S100A4 transcription in EC. ERRγ knockdown inhibited S100A4 expression and promoted the expression of its downstream target E‑cadherin, and vice versa. Mechanistic studies indicated that ERRγ enhanced the promoter activity of S100A4 to facilitate its transcription. In addition, knockdown of ERRγ suppressed migration and invasion of EC cells in vitro, while ectopic ERRγ expression promoted migration and invasion of EC cells in vitro and tumor growth in vivo. Importantly, restoration of S100A4 expression prevented EC cells from undergoing ERRγ‑mediated changes in these biological features. In a...
Scientific reports, Jan 18, 2018
Studies have shown that calcium sensing receptor (CaSR) is involved in the progressions of severa... more Studies have shown that calcium sensing receptor (CaSR) is involved in the progressions of several human cancers. However, the role of CaSR in endometrial cancer remains unknown. This study provides a preliminary analysis of the CaSR effect on endometrial cancer development. Ectopic CaSR expression by lentiviral transfection (CaSR-OV) in Ishikawa cells significantly increased intracellular calcium ([Ca]) levels and cell apoptosis. E-cadherin and β-catenin expression and complex formation at the membrane were increased in CaSR-OV Ishikawa cells relative to control Ishikawa cells (vector). Furthermore, CaSR-OV Ishikawa cells showed a reduced invasive potential, which was attributed to E-cadherin/β-catenin complex formation. Moreover, a reduction in CaSR expression in endometrial cancer relative to normal specimens was evident by immunohistochemistry and was positively associated with E-cadherin, but not β-catenin, expression. Furthermore, VEGFR3 was significantly down-regulated in CaS...