Tias Pramesti Griana - Academia.edu (original) (raw)

Papers by Tias Pramesti Griana

Journal of Islamic Medicine

Onikomikosis merupakan infeksi jamur pada kuku yang disebabkan oleh jamur golongan dermatofita. P... more Onikomikosis merupakan infeksi jamur pada kuku yang disebabkan oleh jamur golongan dermatofita. Penelitian potensi daun kosambi (Schleicera oleosa) sebagai kandidat agent anti jamur untuk penyakit onikomikosis (infeksi jamur pada kuku) telah dilakukan. Daun kosambi (Schleicera oleosa) diekstrak menggunakan methanol. Telah diketahui ekstrak methanol daun kosambi berpotensi sebagai antijamur. Penelitian ini bertujuan membuktikan ekstrak daun Kosambi (Schleicera oleosa (Lour.) Oken) mampu menghambat pertumbuhan Trichophyton spp dan membuktikan daya hambat ekstrak daun Kosambi (Schleicera oleosa (Lour.) Oken) terhadap pertumbuhan Trichophyton spp. lebih baik daripada daya hambat obat ketokonazol sebagai drug of choice penyakit Onikomikosis. Pengujian dilakukan dengan metode cakram dengan perlakuan konsentrasi 5%, 10%, 15%, 20%, 25%, control negatif dengan DMSO 1% dan control positi ketokonazol 2%. Hasil menunjukkan bahwa ekstrak methanol daun kosambi mampu menghambat pertumbuhan Tricho...

Berikut merupakan langkah-langkah untuk mengakses aplikasi Konsul Dokter Covid-19. Aplikasi ini m... more Berikut merupakan langkah-langkah untuk mengakses aplikasi Konsul Dokter Covid-19. Aplikasi ini merupakan aplikasi Konsul Dokter Covid-19 yang digunakan oleh Civitas Akademika UIN Maulana Malik Ibrahim Malang ketika akan memasuki kawasan kampus maupun saat berada didalam kampus yang berbasis website dan dapat diakses secara online melalui laman website https://kedokteran.uin-malang.ac.id/konsuldokter/ dengan menggunakan browser pada computer atau perangkat mobile lain yang dapat terhubung ke interne

Fakultas Kedokteran dan Ilmu Kesehatan, Jul 3, 2020

Puji syukur kita panjatkan kehadirat Allah SWT, karena atas limpahan rahmat, hidayah dan taufik-N... more Puji syukur kita panjatkan kehadirat Allah SWT, karena atas limpahan rahmat, hidayah dan taufik-Nya, Kami dapat menyelesaikan penyusunan Panduan Skripsi Program Studi Pendidikan Dokter. Shalawat serta salam kita haturkan kepada junjungan kita Nabi Muhammad, beserta sahabat dan keluarganya. Ucapan terimakasih kami sampaikan kepada semua pihak yang telah membantu dan memberi masukan dalam penyusunan Panduan Skripsi ini. Panduan Skripsi ini merupakan revisi dari Panduan Skripsi tahun 2019. Upaya perbaikan telah dilakukan untuk menyempurnakan Panduan Skripsi ini, namun tidak menutup kemungkinan masih terdapat kekurangan. Oleh karena itu, kami mengharapkan saran dan masukan dari berbagai pihak guna perbaikan selanjutnya.

Journal of Food and Pharmaceutical Sciences, Jun 19, 2019

The immune system functions to protect the body from pathogens and cure disease. Even a mild infe... more The immune system functions to protect the body from pathogens and cure disease. Even a mild infection will have a bad effect if the host's immune system is not working properly. Modification of the immune response functions to increase and decrease the immune response to overcome disease known as immunomodulation. Immunomodulators are used either as a prevention or treatment of various diseases related to immune disorders. Immunomodulatory drugs change the immune system response by increasing (immunostimulating) or decreasing (immunosuppressive) antibody production. Immunostimulators are given to increase the immune response to infectious diseases, immunodeficiency (e.g. AIDS) and cancer. Immunosuppressive drugs are used to reduce the immune response to transplanted organs and treat autoimmune diseases. The use of medicinal plants to treat various diseases has shown minimal side effects. Natural compounds that are produced from the metabolism of medicinal plants are known to affect the immune system, both the function and production of immune cells, as well as antibody secretion. Indonesia is one of the countries that has used plants as traditional medicine since ancient times. Indonesia 7,000 species of medicinal plants that have been studied to address health problems. Among the medicinal plants that have been studied are Pegagan (Centella asiatica (L.) Urban) and Widuri (Calotropis gigantea (L.)) which have been shown to cure various diseases. This review article describes the potential of native Indonesian medicinal plants, namely Pegagan (Centella asiatica (L.) Urban) and Widuri (Calotropis gigantea (L.)) as immunomodulators.

UIN-Maliki Press eBooks, Nov 1, 2020

Buku The Journey To Normal: Panduan Adaptasi Kebiasaan Baru Pada Masa Pandemi Covid-19 merupakan ... more Buku The Journey To Normal: Panduan Adaptasi Kebiasaan Baru Pada Masa Pandemi Covid-19 merupakan intisari dari panduan adaptasi kebiasaan baru yang telah dikeluarkan oleh Badan kesehatan Dunia (WHO) maupun Pemerintah Indonesia melalui Gugus Tugas Percepatan Penanganan Covid-19 Republik Indonesia. Seperti halnya yang sudah diketahui bersama, pandemic COVID-19 telah melanda hampir seluruh umat manusia di berbagai belahan dunia. Pada tanggal 12 Februari 2020, WHO resmi menetapkan penyakit novel coronavirus pada manusia ini dengan sebutan Coronavirus Disease (COVID19). Pada tanggal 11 Maret 2020, WHO sudah menetapkan COVID-19 sebagai pandemi. Panduan ini ditujukan bagi seluruh cicitas akademika penyelenggara pendidikan tinggi di Indonesia. Berisi tentang pengetahuan dasar infeksi SARS-CoV-2, Ptofisiologi, tatalaksana dan pencegahan serta rehabilitasi pasien Covid-19. Di dalam buku ini juga, penulis menyediakan panduan bagi institusi penyelenggara pendidikan tinggi dalam menyelenggarakan pembelajaran secara luring (luar jaringan). Pimpinan institusi dapat mengaplikasikan prosedur dan kegiatan mitigasi infeksi covid-19 di tempat masing-masing

JPPM (Jurnal Pengabdian dan Pemberdayaan Masyarakat), 2021

Pos Pembinaan Terpadu (Posbindu) Penyakit Tidak Menular (PTM) merupakan bentuk peran serta masyar... more Pos Pembinaan Terpadu (Posbindu) Penyakit Tidak Menular (PTM) merupakan bentuk peran serta masyarakat dalam upaya deteksi dini, pemantauan dan pengendalian faktor risiko PTM yang dilaksanakan secara terpadu, rutin, dan periodik serta mandiri dan berkesinambungan. Dewan Masjid Indonsia (DMI) berkeinginan agar semua masjid di kecamatan Turen memiliki Posbindu PTM untuk memelihara kesehatan masyarakat di sekitarnya. Tujuan pengabdian masyarakat ini untuk menginisiasi pembentukan kader Posbindu PTM berbasis masjid di kecamatan Turen dan meningkatkan kualitas kemampuan kader. Digunakan metode Asset Based Community Development, dengan langkah awal dilakukan survey dan Focus Group Discussion dengan pengurus DMI dan takmir masjid. Dilakukan pengamatan pelaksanaan Posbindu PTM, survei tingkat pengetahuan dengan kuesioner dan pelatihan bagi kader dan calon kader. Hasil penilaian kuesioner tingkat pengetahuan didapatkan 73,13% kader Posbindu PTM dapat menjawab 60% soal yang diberikan dengan be...

Journal of Applied Pharmaceutical Science, 2017

Macrosolen cochinensis is a parasite grows in Indonesia and is known to possess the anticancer ac... more Macrosolen cochinensis is a parasite grows in Indonesia and is known to possess the anticancer activity. This study aims to explain the anticancer activity of the extracts and fractions of jackfruit mistletoe leaves and describe the cell cycle inhibition and induction of apoptosis on T47D breast cancer cell line. The jackfruit mistletoe is extracted using the maceration method. The extract is then separated by liquid-liquid partition method with n-hexan, chloroform, ethyl acetate, and water solvent. MTT method is used to determine the anticancer activity of crude extract and fractions. Cell cycle inhibition test is conducted using flow cytometry with PI marker. The induction of apoptosis is determined by flow cytometry method using PI-Annexin V and PI double staining acridine orange-ethidium bromide. The results indicated that the IC 50 value of the ethanol extract, chloroform fractions and ethyl acetate fractions of jackfruit mistletoe leaves showed a higher anticancer activity with IC 50 which respectively are 362.8, 356.8, 314.8μg/ml. The treatment of n-hexan and water fraction show no signs of anticancer activity because it has a great value of IC 50 which are respectively 926.0 and 2243 μg/ml. Cell death caused by treatment of ethyl acetate fraction of jackfruit mistletoe leaf (Macrosolen cochinensis) is due to the induction of cell apoptosis and cell cycle inhibition in G0-G1, S and G2-M phase.

Diabetes Mellitus merupakan penyakit kronis yang jumlah penderitanya di dunia semakin meningkat. ... more Diabetes Mellitus merupakan penyakit kronis yang jumlah penderitanya di dunia semakin meningkat. Indonesia menempati urutan ke-4 dengan jumlah penderita Diabetes terbesar di dunia setelah India, Cina dan Amerika Serikat. Pegagan (Centella asiatica (L.) Urban) merupakan salah satu tanaman obat yang digunakan oleh masyarakat Indonesia untuk mengatasi penyakit Diabetes. Pegagan digunakan sebagai obat trasisional baikdalam bentuk bahan segar, kering maupun rebusan. Pada penelitian sebelumnya pemberian ekstrak etanol Centella asiatica dapat menurunkan kadar gula darah memperbaiki kerusakan jaringan pankreas. Tujuan dari penelitian ini adalah untuk mengetahui apakah pegagan (Centella asiatica (L.) Urban) yang diolah secara tradisional mampu memperbaiki gambaran histologi pankrean tikus putih (Rattus norvegicus) yang dibuat menderita Diabetes Mellitus kronis dengan induksi aloksan. Dalam penelitian ini digunakan 2 kontrol sampel, yaitu kontrol positif (K+) dan kontrol neatif (K-). Sedangka...

Indonesia merupakan negara kepulauan dengan berbagai suku dan budaya. Perbedaan budaya menyebabka... more Indonesia merupakan negara kepulauan dengan berbagai suku dan budaya. Perbedaan budaya menyebabkan berbeda jenis makanan khas yang ada di Indonesia. Makanan hasil pengolahan dengan fermentasi dapat dijumpai hampir di setiap daerah. Pengolahan makanan dengan bioteknologi konvensional yang berupa fermentasi melibatkan berbagai mikroorganisme. Senyawa hasil metabolisme mikroba yang hidup pada bahan baku pangan merupakan prebiotik yang memiliki manfaat bagi kesehatan. Begitu pula mikroba yang hidup pada makanan terfermentasi yang masuk ke dalam tubuh, disebut sebagai probiotik, mampu meningkatkan fungsi imun. Sehingga makanan terfermentasi memiliki potensi sebagai pengatur sistem imun (imunomodulator) di dalam tubuh manusia. Artikel ini mengulas potensi bahan makanan dengan pengolahan fermentasi yang berasal dari berbagai daerah di Indonesia sebagai agen imunomodulator.

The effect of Calotropis gigantea (CG) leaves extract on fibrosarcoma growth and Caspase 3 expres... more The effect of Calotropis gigantea (CG) leaves extract on fibrosarcoma growth and Caspase 3 expression have been evaluated. Mile mice were divided into 5 groups with 7 mice per group, (1) Negative control (CMC-Na 0.5%), (2) Positive control (Methotrexate 2,5 mg/kgBW), (3) 50 mg/Kg BW of CG, (4) 100 mg/Kg BW of CG, (5) 150 mg/Kg BW of CG. Mice body weights were observed before induced by cancer agent. Then, all mice were injected by 0.1 mL DMBA 25 μg/0.1 mL acetone by subcutaneous injection two times a week for 6 weeks. After treatment, all mice were sacrificed by euthanasia method based on Guideline for the Euthanasia of Animals. Then, mice nodules were taken and washed with PBS. Nodules were weighted and kept in the formaldehyde solution. Then immunohistochemical caspase-3 specimens were made. C. gigantea leaves extract at a dose of 100 and 150 mg/Kg BW were able to inhibit the growth of fibrosarcoma in experimental animals induced DMBA. This extract also improved the apoptotic inde...

Colorectal cancer is the third most commonly diagnosed cancer in males and the second in females,... more Colorectal cancer is the third most commonly diagnosed cancer in males and the second in females, with 1.4 million new cases and almost 694,000 deaths estimated to have occurred in 2012. Colorectal cancer is an emerging public health problem in Indonesia and currently ranks among the three highest cancers . The age standardized incidence rates of colorectal cancer per 100.000 populations in Indonesia were 19.1 for men and 15.6 for women. Previous epidemiological studies have shown that colorectal cancer patients in Indonesia are younger than patients in developed countries. More than 30% of cases were 40 years old or younger , while patients younger than 50 years in developed countries accounted for only 2-8% . In addition, where as young or early onset colorectal cancer in developed countries are often associated with familial cases , most Indonesian colorectal cancer patients are determined to be sporadic. With the increasing number of cases and the occurrence of colorectal cancer...

Kegagalan yang sering terjadi dalam pengobatan kanker, utamanya melalui kemoterapi adalah disebab... more Kegagalan yang sering terjadi dalam pengobatan kanker, utamanya melalui kemoterapi adalah disebabkan karena rendahnya selektifitas obat-obat antikanker terhadap sel normal sehingga menimbulkan efek samping yang serius pada pasien. Selain itu kegagalan kemoterapi tersebut juga disebabkan karena resistensi sel kanker terhadap agen-agen kemoterapi. Fenomena resistensi tersebut membawa konsekwensi pada semakin meningkatnya dosis terapi (Conze et al, 2001). Oleh karena hal itu sampai saat ini masih perlu dikembangkan obat kemoterapi baru dalam mengatasi permasalahan tersebut di atas. Salah satu alternatif bahan obat yang bisa digunakan adalah dari bahan alam. Salah satu bahan alam yang menarik untuk diteliti adalah tanaman widuri (Calotrophis gigantea). Berdasar data peneitian sebelumnya didapatkan bahwa Fraksi etylasetat daun widuri mempunyai aktivitas antikanker paling kuat (IC50: 33.08 μg/mL) dan dosis yang berefek antikanker tersebut masih dalam batas aman terhadap pertumbuhan sel no...

Objective : The objective of this study was to examine whether Calotropis gigantea leaf extract (... more Objective : The objective of this study was to examine whether Calotropis gigantea leaf extract (CGLE) synergizes the therapeutic potential of 5-flourouracil and Doxorubicin against colon cancer WiDr cell lines. Methods : MTT assay was used to measure the growth inhibitory effect of the combination. Results : CGLE 3 µg/ml which was combined with 5-FU 62.5 µg/ml, 125 µg/ml, and 250 µg/ml, synergistically inhibited the growth of WiDr colon cancer cells line with Combination Index value (CI) ranging from 0.20 to 0.07. In contrast, combination of CGLE with Doxorubicin was given antagonistically inhibited on the growth of colon cancer WiDr cell line, with Combination Index value (CI) ranging from 0,9 to 3,3 (moderate antagonis effect until highly antagonist effect). Conclusion : Combination therapy of CGLE showed strong synergism with 5-FU and strong antagonism with doxorubicin in inhibiting the growth of human colon cancer WiDr cells line.

Pharmacognosy Journal, 2020

Objective: This study aimed to investigate the immunomodulatory capacity of goat kefir on pulmona... more Objective: This study aimed to investigate the immunomodulatory capacity of goat kefir on pulmonary fibrosis rat model. Material and Methods: Twenty-five male rats were randomly divided into five groups: one group only received induction with bleomycin (0.3 mg/rat) to induce pulmonary fibrosis; three groups were treated with different doses (2.5, 3.5, and 4.5 mL/200 g BW) of goat kefir, following the induction with bleomycin, for 30 days; and one group served as negative control, did not receive bleomycin induction as well as kefir. On day 30, all the animals were sacrificed. Plasma levels of TGF-β, IL-4, and IFN-y were measured using the ELISA method, and the expression of α-SMA in myofibroblast cells was examined with the help of immunohistochemistry assay. Results: Induction with bleomycin significantly elevated the expressions of TGF-β, IL-4, and IFN-y in comparison to the control group. Following the administration of kefir (3.5 and 4.5 mL/200 g BW), the concentration of TGF-β was significantly decreased (p<0.05); whereas, the concentration of IFN-y increased slightly (p<0.05) only in the group that received the 4.5 mL/200 g BW dose of kefir. In contrast, IL-4 exhibited increasing levels with higher doses of kefir (p<0.05). The expression of α-SMA in myofibroblasts showed a tendency to decline following the administration of kefir, although this decline was not statistically significant. Conclusions: Goat kefir caused a reduction in the TGF-β levels in fibrosis conditions; however, the kefir elicited an immunosuppressive effect during the progression of the pulmonary fibrosis.

Indonesian Journal of Pharmacy, 2020

It is known that Eleutherine palmifolia (L.) Merr contains flavonoid and polyphenol as bioactive ... more It is known that Eleutherine palmifolia (L.) Merr contains flavonoid and polyphenol as bioactive compounds that have the ability as chemopreventive agents. This study aims to examine the effect of Eleutherine palmifolia (L.) Merr extract (EPE) on colon histopathology and the enhancement of caspase-3 expression in BALB/c mice of colitis-associated colon cancer (CAC) model. Thirty Balb/c female mice were used in this study and were divided into six groups. Five mice in one group were normal or negative control (given phosphate-buffered saline), and twenty-five mice were induced intraperitoneally with 10 mg/kg BW AOM (Azoxymethane), and it was followed by the administration of 5% DSS (Dextran Sodium Sulfate) every two weeks for 20 weeks. At the sixth week, one mice in each group was sacrificed for the Fecal Occult Bold Test (FOBT) and serum amyloid α (SAA) test to ensure that CAC was indeed formed. The administration of EPE with varying doses was started from the eighth week and was co...

Jurnal Kedokteran dan Kesehatan : Publikasi Ilmiah Fakultas Kedokteran Universitas Sriwijaya, 2021

Proses penyembuhan luka pada kulit akibat injuri terdiri dari 3 fase, fase inflamasi, fase prolif... more Proses penyembuhan luka pada kulit akibat injuri terdiri dari 3 fase, fase inflamasi, fase proliferasi dan fase remodeling. Schleichera oleosa memiliki senyawa aktif yang mampu menghambat proses inflamasi. penelitian ini bertujuan untuk mengetahui efek aplikasi topikal ekstrak daun kesambi untuk penyembuhan luka pada mencit. 30 mencit jantan yang dieksisi kulit punggungnya, dibagi menjadi 5 kelompok, kontrol negatif (K-) yang tidak diberi apa-apa, kelompok perlakuan yang diberi salep ekstrak daun kesambi dengan konsentrasi 0% (P0), 5% (P1), 10% (P2) dan 15% (P3). Sebelum diaplikasikan, salep di evaluasi organoleptik, homogenitas, pH dan viskositasnya. Setelah diaplikasikan ke luka kulit pada mencit, diamati eritema yang terjadi di kulit yang sehat sekitar luka, adanya krusta dan granulasi pada hari ke-28. Pengukuran persentase konstriksi dan intensitas warna eritema dilakukan pada hari ke-1, ke-7, ke-14, ke-21 dan ke-28. Hasil penelitian menunjukkan bahwa semua sediaan salep memiliki bentuk fisik yang baik, pH sesuai untuk kulit, sedangkan berdasarkan SNI, hanya salep dengan ekstrak daun kesambi 15% yang memiliki viskositas baik. Aplikasi sediaan salep ekstrak daun kesambi tidak menimbulkan iritasi pada kulit mencit yang sehat, dan tidak ditemukan krusta atau granulasi pada luka. Persentase konstriksi luka yang tertinggi adalah pada kelompok P3, sedangkan instensitas warna eritema tidak memiliki perbedaan pada semua kelompok mencit.

Journal of Islamic Pharmacy, 2020

Kesambi (Schleichera oleosa) is one of the forest plants from Indonesia that has potential as med... more Kesambi (Schleichera oleosa) is one of the forest plants from Indonesia that has potential as medicine. Kesambi has active compounds that act as antioxidants. The purpose of this study was to determine the potential of kesambi leaf as an antioxidant using the DPPH method. Samples of kesambi leaf were extracted in methanol, ethanol, and water solvents by the maceration method. The class of compounds contained in kesambi leaf was tested by the colour reaction test method for alkaloids, flavonoids, triterpenoids, phenols, steroids, saponins, and tannins. Antioxidant activity was tested by the DPPH method to determine IC50 values. Based on the results of the colour reaction test showed that methanol and ethanol solvents were able to bind 6 classes of compounds (alkaloids, flavonoids, triterpenoid, phenols, saponins, and tannins) and water solvents were only able to bind 5 classes of compounds (flavonoids, triterpenoid, phenols, saponins, and tannins) contained in kesambi leaf. The results of antioxidant activity tests using the DPPH method showed the lowest IC50 value was methanol extract (16,12µg/ml) compared to ethanol extract (20,43µg/ml) and water (904,28µg/ml). It was concluded that the leaf extract of kesambi has potential as an antioxidant and which has the best antioxidant ability was extracted in methanol. The class of compounds which were thought to be responsible for the antioxidant activity of the kesambi leaf extract based on the color reaction test were phenols, flavonoids and tannins.

Journal of Islamic Pharmacy, 2019

The root of Calotropis gigantea (thistle) is an Indonesian herb to treat cancer based on empirica... more The root of Calotropis gigantea (thistle) is an Indonesian herb to treat cancer based on empirical and scientific evidences. The study aims to find out the infuence of ethanol extract of Calotropis gigantea root in inhibiting cancer cell growth of Mus musculus fibrosarcoma in vivo and to find out metabolite compound in the Calotropis gigantea root extract. The effect of cancer cell growth inhibition was tested on Mus musculus inducted with 7.12-dimetilbenz (α) antrasena (DMBA) and dose treatment of 50, 100 and 150 mg/Kg body weight. The metabolomic analysis on the root employs UPLC-QToF-MS/MS as a positive ESI ion source, the movement phase of water/acid mixture is 99.9/0.1 [v/v] and acetonitrile/formic acid was 99.9/0.1 [v/v] with gradient elution system and stationary phase of C18. The chromatogram was analyzed using Masslynx 4.1. The component identification was based on the m/z ratio measured in Masslynx and m/z counted in chemdraw. The result of the study showed that the Calotr...