T. Thomson - Academia.edu (original) (raw)

Papers by T. Thomson

Research paper thumbnail of Cell-cycle dependent subcellular distribution and mitogenic effects of PTOV1, a novel protein overexpressed in prostate cancer

European Urology Supplements, 2003

Research paper thumbnail of Induction of Ornithine Decarboxylase Activity (Odc) by Oncogene N-Ras in a Ras-Responsive Neurodifferentiating Cell-Line

Page 1. 1983;43:5174-5177. Published online November 1, 1983. Cancer Res Michael J. Connor and Ni... more Page 1. 1983;43:5174-5177. Published online November 1, 1983. Cancer Res Michael J. Connor and Nicholas J. Lowe Synthesis in Hairless Mouse Epidermis by Retinoids Induction of Ornithine Decarboxylase Activity and DNA ...

Research paper thumbnail of Positive autoregulation of ras genes expression in fibroblasts

FEBS Letters, 1997

We have studied the effect of ectopie overexpression of a ras gene on the expression of the other... more We have studied the effect of ectopie overexpression of a ras gene on the expression of the other two members of the ras gene family. We obtained NIH3T3 cell lines stably transfected with inducible H-ras and N-ras oncogenes. The expression of these genes is driven by a glucocorticoid-responsive promoter and the addition of dexamethasone resulted in a dramatic induction (10-20-fold) of H-or N-ras mRNA, peaking 4 h after hormone addition. The induction of the expression of ras oncogenes resulted in a transformed phenotype. In quiescent NIH3T3 cells transfected with inducible H-ras oncogenes, the induction of H-Ras was followed 12 h later by a 3-fold increase in the mRNA expression of endogenous K-ras and N-ras. Similarly, in NIH3T3 transfected with inducible N-ras oncogene, the induction of N-ras was followed by an increase in the expression of endogenous K-and H-ras genes. Interestingly, the effect was not limited to the mutated N-ras, as a similar result was obtained in cells transfected with N-ras proto-oncogene. The induction of ras genes expression was not linked to cell cycle progression as it was reproduced in cells arrested in S-phase by pretreatment with hydroxyurea. These results suggest the presence of a positive cross-regulation in the expression among the members of the Ras family. This effect could play a role in Ras-mediated carcinogenesis.

Research paper thumbnail of Cell surface antigens of human bladder cancer defined by mouse monoclonal antibodies

Proceedings of the …, 1984

Mouse monoclonal antibodies were obtained by immunization with cultured human bladder cancer or l... more Mouse monoclonal antibodies were obtained by immunization with cultured human bladder cancer or lysates of bladder papilloma. They identify 11 distinct antigenic systems as defined by serological analysis of cultured cells and studies of antigen distribution in normal and neoplastic tissues. The most restricted of these antigens, OmS, defines a subset of bladder tumors. Om5 is not detected in normal bladder urothelium or in any other normal or malignant tissue. T101 and JP165 are also subset markers for bladder cancer that are not detected in normal tissues. T16, T43, T87, and J143 (antigens represented on many cultured cells) are found in specific areas of the normal urinary tract and in a distinctive range of other normal and malignant cell types-e.g., T16 expression in pluristratifiled epithelium of skin, exocervix, and esophagus. T138 antigen is also a common feature of cultured cell lines, but its expression in sections of normal tissues is restricted to endothelial cells. In contrast, T110 is poorly represented on cultured cells but can be detected in culture supernatants. Localization of T10 in normal tissues showed that it is a component of the extracellular matrix. All determinants detected by this series of antibodies are heat labile and not related to A, B, H, I, Lewis blood group antigens. Six of the antibodies immunoprecipitated glycoproteins from radiolabeled cell lysates:

Research paper thumbnail of Anti-Golgi complex antibodies in a patient with Sjögren's syndrome and lymphoma

Clinical & Experimental Immunology

During routine immunofluorescence studies of the serum of a patient with Sj6gren's syndrome and l... more During routine immunofluorescence studies of the serum of a patient with Sj6gren's syndrome and lymphoma we detected antibodies giving a cytoplasmic pattern which did not correspond to previously described patterns found for autoantibodies. Using different cells and tissues as substrates for indirect immunofluorescence, including rat liver, rat small bowel, rat testicle, human thyroid, guinea-pig plasma cells and cultured human fibroblasts, the cytoplasmic structure to which these autoantibodies are directed seems to be the golgi complex, a conclusion supported by histochemical studies. Furthermore, these antibodies were absorbed by isolated golgi vesicles. The autoantibodies are of IgG and IgA classes, and the antigen(s) with which they react is(are) resistant to treatment with DNAase and RNAase. None of the sera from 50 normal individuals, seven patients with Sj6gren's syndrome (five of them primary and two associated with rheumatoid arthritis; none of them with lymphoma), 25 patients with mixed connective tissue disease, 10 patients with systemic lupus erythematosus and five patients with progressive systemic sclerosis, had antibodies directed against this cytoplasmic specificity, as determined by indirect immunofluorescence. This is the first time that autoantibodies directed to the golgi complex are reported. The significance of this finding awaits further descriptions in patients with a clinical picture similar to the one reported here.

Research paper thumbnail of Cell-cycle dependent subcellular distribution and mitogenic effects of PTOV1, a novel protein overexpressed in prostate cancer

European Urology Supplements, 2003

Research paper thumbnail of Induction of Ornithine Decarboxylase Activity (Odc) by Oncogene N-Ras in a Ras-Responsive Neurodifferentiating Cell-Line

Page 1. 1983;43:5174-5177. Published online November 1, 1983. Cancer Res Michael J. Connor and Ni... more Page 1. 1983;43:5174-5177. Published online November 1, 1983. Cancer Res Michael J. Connor and Nicholas J. Lowe Synthesis in Hairless Mouse Epidermis by Retinoids Induction of Ornithine Decarboxylase Activity and DNA ...

Research paper thumbnail of Positive autoregulation of ras genes expression in fibroblasts

FEBS Letters, 1997

We have studied the effect of ectopie overexpression of a ras gene on the expression of the other... more We have studied the effect of ectopie overexpression of a ras gene on the expression of the other two members of the ras gene family. We obtained NIH3T3 cell lines stably transfected with inducible H-ras and N-ras oncogenes. The expression of these genes is driven by a glucocorticoid-responsive promoter and the addition of dexamethasone resulted in a dramatic induction (10-20-fold) of H-or N-ras mRNA, peaking 4 h after hormone addition. The induction of the expression of ras oncogenes resulted in a transformed phenotype. In quiescent NIH3T3 cells transfected with inducible H-ras oncogenes, the induction of H-Ras was followed 12 h later by a 3-fold increase in the mRNA expression of endogenous K-ras and N-ras. Similarly, in NIH3T3 transfected with inducible N-ras oncogene, the induction of N-ras was followed by an increase in the expression of endogenous K-and H-ras genes. Interestingly, the effect was not limited to the mutated N-ras, as a similar result was obtained in cells transfected with N-ras proto-oncogene. The induction of ras genes expression was not linked to cell cycle progression as it was reproduced in cells arrested in S-phase by pretreatment with hydroxyurea. These results suggest the presence of a positive cross-regulation in the expression among the members of the Ras family. This effect could play a role in Ras-mediated carcinogenesis.

Research paper thumbnail of Cell surface antigens of human bladder cancer defined by mouse monoclonal antibodies

Proceedings of the …, 1984

Mouse monoclonal antibodies were obtained by immunization with cultured human bladder cancer or l... more Mouse monoclonal antibodies were obtained by immunization with cultured human bladder cancer or lysates of bladder papilloma. They identify 11 distinct antigenic systems as defined by serological analysis of cultured cells and studies of antigen distribution in normal and neoplastic tissues. The most restricted of these antigens, OmS, defines a subset of bladder tumors. Om5 is not detected in normal bladder urothelium or in any other normal or malignant tissue. T101 and JP165 are also subset markers for bladder cancer that are not detected in normal tissues. T16, T43, T87, and J143 (antigens represented on many cultured cells) are found in specific areas of the normal urinary tract and in a distinctive range of other normal and malignant cell types-e.g., T16 expression in pluristratifiled epithelium of skin, exocervix, and esophagus. T138 antigen is also a common feature of cultured cell lines, but its expression in sections of normal tissues is restricted to endothelial cells. In contrast, T110 is poorly represented on cultured cells but can be detected in culture supernatants. Localization of T10 in normal tissues showed that it is a component of the extracellular matrix. All determinants detected by this series of antibodies are heat labile and not related to A, B, H, I, Lewis blood group antigens. Six of the antibodies immunoprecipitated glycoproteins from radiolabeled cell lysates:

Research paper thumbnail of Anti-Golgi complex antibodies in a patient with Sjögren's syndrome and lymphoma

Clinical & Experimental Immunology

During routine immunofluorescence studies of the serum of a patient with Sj6gren's syndrome and l... more During routine immunofluorescence studies of the serum of a patient with Sj6gren's syndrome and lymphoma we detected antibodies giving a cytoplasmic pattern which did not correspond to previously described patterns found for autoantibodies. Using different cells and tissues as substrates for indirect immunofluorescence, including rat liver, rat small bowel, rat testicle, human thyroid, guinea-pig plasma cells and cultured human fibroblasts, the cytoplasmic structure to which these autoantibodies are directed seems to be the golgi complex, a conclusion supported by histochemical studies. Furthermore, these antibodies were absorbed by isolated golgi vesicles. The autoantibodies are of IgG and IgA classes, and the antigen(s) with which they react is(are) resistant to treatment with DNAase and RNAase. None of the sera from 50 normal individuals, seven patients with Sj6gren's syndrome (five of them primary and two associated with rheumatoid arthritis; none of them with lymphoma), 25 patients with mixed connective tissue disease, 10 patients with systemic lupus erythematosus and five patients with progressive systemic sclerosis, had antibodies directed against this cytoplasmic specificity, as determined by indirect immunofluorescence. This is the first time that autoantibodies directed to the golgi complex are reported. The significance of this finding awaits further descriptions in patients with a clinical picture similar to the one reported here.