Takahiro Tajino - Academia.edu (original) (raw)

Papers by Takahiro Tajino

Background and purpose. Giant cell tumor (GCT) of the small bones (small-bone GCT) is usually rar... more Background and purpose. Giant cell tumor (GCT) of the small bones (small-bone GCT) is usually rare and considered somewhat different from conventional GCT. The purpose of this study was to investigate and report the clinicopathological features of 11 cases with small-bone GCT. Materials and methods. Patient information was obtained with the help of questionnaires. X-rays and paraffin blocks obtained from several institutions were clinically, radiographically, and histologically evaluated. Results. Small-bone GCT was observed in younger patients compared to conventional GCT; 5 of the 11 (45%) patients were below 20 years of age, whereas the corresponding figure for all GCT patients is 16 % in Japan. Excessive cortical bone expansion is a special feature. There were two cases of recurrence and one case of lung metastasis; the primary lesion was in the hand for all three cases. In contrast, no primary lesion of the foot recurred or metastasized. Varying degrees of positive p63 immunost...

PubMed, Aug 6, 2005

The aims of this study were to investigate the anti-tumor effects of consecutive low-dose cisplat... more The aims of this study were to investigate the anti-tumor effects of consecutive low-dose cisplatin (LD-CDDP) in comparison with single high-dose CDDP (HD-CDDP) in combination with caffeine. Materials and methods: Human fibrosarcoma cells (HT-1080) were transplanted in BALB/C-nu mice. According to the administration of CDDP and caffeine, 5 groups were defined: HD-CDDP, LD-CDDP, HD-CDDP +caffeine, LD-CDDP +caffeine and control group. The total dose of CDDP was 3.5 or 6 mg/kg. CDDP was injected i.p. bolus (HD-CDDP) or divided into 5 days (LD-CDDP). Caffeine was injected i.p. at a dose of 60 mg/kg twice a day for 4 days. Results: Significant inhibition of tumor growth and prolongation of survival time were recognized in the HD- and LD-CDDP groups with and without caffeine compared to the control group. The caffeine-assisted groups had no advantage compared to the CDDP alone groups. Conclusion: The effects of LD-CDDP were similar to the effect of HD-CDDP.

PubMed, Nov 11, 2006

Background: Undifferentiated pleomorphic sarcoma (UPS) is among the most common soft tissue sarco... more Background: Undifferentiated pleomorphic sarcoma (UPS) is among the most common soft tissue sarcomas in adults. In order to improve its aggressive course or prognosis and establish new therapeutic methods, molecular genetic and biological characterizations of UPS are required. Materials and methods: A new human UPS cell line (FPS-1) was established from UPS of the upper arm of a 79-year-old man. The cell line has been maintained for over 14 months with more than 60 passages. FPS-1 cells were characterized using molecular biological methods. Results: FPS-1 cells showed the same morphological and immunophenotypical characteristics as the primary tumor. Cytogenetic and molecular analyses revealed a nonsense mutation in exon 6 of the p53 gene. Epidermal growth factor receptor (EGFR) and cyclooxygenase-2 (COX-2) were expressed in FPS-1 cells. Conclusion: FPS-1 cells might be useful for investigating biological behavior and developing new molecular targeting antitumor drugs for UPS with EGFR or COX-2 expression.

Virchows Archiv, Nov 18, 2009

Malignant peripheral nerve sheath tumor (MPNST) is a rare soft tissue sarcoma. We established a n... more Malignant peripheral nerve sheath tumor (MPNST) is a rare soft tissue sarcoma. We established a new human MPNST cell line (designated FMS-1) from MPNST of the right brachial plexus of a 69-year-old woman with NF1. The cell line has been maintained for >24 months with >100 passages. FMS-1 cells showed a fibrosarcoma-like or epithelioid pattern in the heterotransplanted tumor, compared with a fascicular growth pattern of short-spindle tumor cells in the primary tumor. Immunophenotypically, FMS-1 cells showed almost the same characteristics as the primary tumor. Cytogenetic and molecular analyses revealed a deletion in exons 5-8 of the p53 gene. Epidermal growth factor receptor (EGFR) and cyclooxygenase (COX)-2 were expressed in FMS-1 cells. To improve the highly aggressive course and poor prognosis and establish new therapeutic methods, molecular genetic and biological characterizations of MPNST are required. Thus, FMS-1 cells might be useful for investigating biological behaviors and developing new molecular-targeting antitumor drugs for MPNST expressing EGFR or COX-2.

Medical Principles and Practice, Sep 22, 2012

Remitting seronegative symmetrical synovitis with pitting edema (RS3PE) syndrome is an uncommon d... more Remitting seronegative symmetrical synovitis with pitting edema (RS3PE) syndrome is an uncommon disorder, characterized by an acute-onset seronegative inflammation with pitting edema in the distal extremities, remitting without functional impairment. RS3PE syndrome is known as a paraneoplastic syndrome caused by some solid cancers and hematologic diseases. However, RS3PE syndrome rarely occurs due to inflammatory disease including infections and crystal-induced arthritis. We describe herein an extremely rare case of gouty arthritis-induced RS3PE syndrome in a 76-year-old man. Case Report The patient was a 76-year-old man with no remarkable past medical history including gout/hyperuricemia. He presented with a 1-month history of swelling and mild pain in the dorsum of his right foot, and a 3-week history of the same symptoms in his left foot. In addition he had a 1-week history of swelling and moderate pain in the dorsum of both of his hands with severe pain in his left wrist. There was no history of trauma. Upon examination, his body temperature was 38.1 ° C and local examination revealed swelling with pitting edema on the dorsum of his hands and feet bilaterally with surrounding erythema Key Words Remitting seronegative symmetrical synovitis with pitting edema syndrome ؒ Crystal-induced arthritis ؒ Gout ؒ Intra-articular corticosteroid injection ؒ Nonsteroidal anti-inflammatory drugs Abstract Objective: To describe a rare case of remitting seronegative symmetrical synovitis with pitting edema (RS3PE) syndrome caused by gouty arthritis. Clinical Presentation and Intervention: A 76-year-old man presented with swelling and pain in the dorsum of feet and hands bilaterally. From the laboratory and radiologic findings, the diagnosis of gout-induced RS3PE syndrome was made. Conservative therapy with nonsteroidal anti-inflammatory drugs (NSAIDs) and intra-articular corticosteroid injection in the wrist joint completely and rapidly resolved all symptoms. The patient was successfully treated with oral administration of NSAIDs and a one-time intra-articular corticosteroid injection in the left wrist joint. Conclusion: This case demonstrated the importance of considering the possibility of crystal-induced arthritis such as gout and pseudogout, as well as malignant disease, when diagnosing the primary disease responsible for RS3PE syndrome.

The American Journal of Surgical Pathology, Dec 1, 2001

ABSTRACT

Anticancer research, 2018

Clear cell sarcoma (CCS) of soft tissue is exceedingly rare and frequently exhibits aggressive be... more Clear cell sarcoma (CCS) of soft tissue is exceedingly rare and frequently exhibits aggressive behavior. Toward the goals of improving the aggressive course and poor prognosis of CCS, and establish new therapeutic methods, molecular genetic and biological characterizations of CCS are required. A new human CCS cell line (designated RSAR001) was established from the pleural effusion of a 44-year-old man with multiple lung metastases and pleural dissemination. The cell line and its xenograft were characterized including their morphology, immunohistochemistry, cytogenetic analysis, reverse transcription-polymerase chain reaction, direct sequencing analysis, and fluorescence in situ hybridization analysis. The cell line has been maintained for over 12 months with more than 50 passages. RSAR001 cells exhibited a fascicular or diffuse growth pattern of short spindle- or oval-shaped cells with clear cytoplasm in heterotransplanted tumor, that was similar to the primary tumor. Immunophenotyp...

Hellenic journal of nuclear medicine, 2016

We describe herein two tumor-induced osteomalacia (TIO) cases for whom the causative lesions, loc... more We describe herein two tumor-induced osteomalacia (TIO) cases for whom the causative lesions, located in their popliteal fossa, that were not identified in the standard field of fluorine-18-fluorodeoxyglucose positron emission tomography/computed tomography ((18)F-FDG PET/CT), which usually images only the head, trunk, and proximal parts of the extremities. A 47 years old Japanese man with multiple pathological fractures due to osteomalacia, accompanied by muscle weakness, hypophosphatemia, and an elevation of alkaline phosphatase (ALP) was referred to our hospital. A (18)F-FDG PET/CT scan was performed, but no (18)F-FDG uptake was detected in the standard field of imaging. Magnetic resonance imaging revealed a small subcutaneous tumor (1.9×1.2×0.6cm) of the left posteriomedial knee, displaying uniform enhancement on gadolinium-enhanced T1-weighted fat-suppression imaging. The tumor was resected widely and diagnosed as phosphaturic mesenchymal tumor, mixed connective tissue variant ...

Anticancer research

The aims of this study were to investigate the anti-tumor effects of consecutive low-dose cisplat... more The aims of this study were to investigate the anti-tumor effects of consecutive low-dose cisplatin (LD-CDDP) in comparison with single high-dose CDDP (HD-CDDP) in combination with caffeine. Human fibrosarcoma cells (HT-1080) were transplanted in BALB/C-nu mice. According to the administration of CDDP and caffeine, 5 groups were defined: HD-CDDP, LD-CDDP, HD-CDDP +caffeine, LD-CDDP +caffeine and control group. The total dose of CDDP was 3.5 or 6 mg/kg. CDDP was injected i.p. bolus (HD-CDDP) or divided into 5 days (LD-CDDP). Caffeine was injected i.p. at a dose of 60 mg/kg twice a day for 4 days. Significant inhibition of tumor growth and prolongation of survival time were recognized in the HD- and LD-CDDP groups with and without caffeine compared to the control group. The caffeine-assisted groups had no advantage compared to the CDDP alone groups. The effects of LD-CDDP were similar to the effect of HD-CDDP.

Ultrastructural Pathology, 2008

Fibronexus (fibronexus junction) has been thought to be a characteristic ultrastructural feature ... more Fibronexus (fibronexus junction) has been thought to be a characteristic ultrastructural feature of myofibroblasts, but it is controversial as to whether fibronexus is a characteristic of various myofibroblastic tumors. We report here a case of low-grade myofibrosarcoma with fibronexus arising in the right arm of an 80-year-old man. Histologically, the tumor was composed of relatively uniform and slender spindle cells arranged in fascicles. The nuclei with fusiform and tapered shapes were mildly hyperchromatic, but never exhibited pleomorphism. Mitotic figures were common, but no atypical mitosis was identified. At the tumor periphery, tumor cells had invaded into the surrounding skeletal muscle tissue. Tumor cells were positive diffusely for alpha-smooth muscle actin and less intensely for desmin, but were negative for h-caldesmon and S-100 protein. Ultrastructurally, tumor cells had well developed cytoplasmic organelles and varying amounts of peripheral or subplasmalemmal bundles of thin myofilaments with focal density. In addition, well formed, long fibronectin fibrils adjacent to the cell surface and fibronexus contacting intracellular myofilaments were easily identified. We believe that fibronexus is a useful ultrastructural feature for differentiating myofibrosarcoma from other myogenic sarcomas.

Histopathology, 2002

Extra-abdominal desmoid fibromatosis: two familial cases with synchronous and metachronous multic... more Extra-abdominal desmoid fibromatosis: two familial cases with synchronous and metachronous multicentric hyalinizing nodules Aims: Extra-abdominal desmoid fibromatosis is an uncommon tumour. We present here two exceptional familial cases of extra-abdominal desmoid fibromatosis, one of which was synchronous and metachronous. Methods and results: The first patient was a 37-year-old woman who had noted a tumour growing on the dorsum of her right foot when she was 12 years old. She underwent excision of the tumour but in the following year the tumour recurred locally and grew into multiple nodules. Subsequently, multicentric tumours appeared in her knee, distal and posterior aspects of her thigh, right back and right anterior shoulder. Polyostotic fibrous dysplasia of the femur and cranium was found on radiological examination. The second patient was a 74-year-old man, the uncle of the first patient. He underwent an excisional operation of a tumour on the internal malleolus surface of his fibula when he was 46 years old. The tumour recurred 7 years later and was excised. His post-operative course has been uneventful. The histology of the primary and recurrent tumours was distinctive and consistently showed hyalinizing scar-like features. Conclusions: Familial cases of extra-abdominal desmoid fibromatosis with extensive multicentric lesions and distinctive hyalinizing scar-like features are described. Recently, attenuated familial adenomatous polyposis with familial desmoid fibromatosis has been recognized, and familial desmoid fibromatosis without adenomatous polyposis may also be one of its variants. Although the present cases have no history of colon polyposis or carcinoma, monitoring of the intestinal tract would seem to be indicated.

Annals of Diagnostic Pathology, 2012

Spindle cell hemangioma, a rare benign tumor characterized by cavernous blood vessels and spindle... more Spindle cell hemangioma, a rare benign tumor characterized by cavernous blood vessels and spindled areas, typically arises in the subcutis of the distal extremities, particularly the hand. The case of intraosseous spindle cell hemangioma is extremely rare, and only 1 case arises in the frontal bone has been reported previously. We describe herein a case of intraosseous spindle cell hemangioma occurring in the left calcaneus in a 65-year-old woman. The patient was successfully treated by the operation. The present case is instructive especially in the differential diagnosis of primary bone tumor structured by spindle cells, for which the possibility of spindle cell hemangioma should be considered.

Australasian Journal of Dermatology

Scientific Reports, 2019

Hepatitis C virus (HCV) cell culture systems have facilitated the development of efficient direct... more Hepatitis C virus (HCV) cell culture systems have facilitated the development of efficient direct-acting antivirals against HCV. Huh-7.5, a subline of the human hepatoma cell line Huh-7, has been used widely to amplify HCV because HCV can efficiently replicate in these cells due to a defect in innate antiviral signalling. Recently, we established a novel cell line, KH, derived from human hepatocellular carcinoma, which showed atypical uptake of gadolinium ethoxybenzyl diethylenetriamine pentaacetic acid (Gd-EOB-DTPA) in a Gd-EOB-DTPA-enhanced magnetic resonance imaging study. KH cells expressed hepatocyte markers including microRNA-122 (miR-122) at a lower level than Huh-7.5 cells. We demonstrated that KH cells could support the entire life cycle of HCV; however, HCV replicated at a lower rate in KH cells compared to Huh-7.5 cells, and virus particles produced from KH cells seemed to have some disadvantages in viral assembly compared with those produced from Huh-7.5 cells. KH cells had more robust interferon-stimulated gene expression and induction upon HCV RNA transfection, interferon-α2b addition, and HCV infection than Huh-7.5 cells. Interestingly, both miR-122 supplementation and IRF3 knockout in KH cells boosted HCV replication to a similar level as in Huh-7.5 cells, suggesting that intact innate antiviral signalling and lower miR-122 expression limit HCV replication in KH cells. KH cells will enable a deeper understanding of the role of the innate immune response in persistent HCV infection.

Archives of Dermatological Research, 1996

Human monocyte/macrophage lineages have unique phagocytic and immune-regulatory functions. We est... more Human monocyte/macrophage lineages have unique phagocytic and immune-regulatory functions. We established a promonocytic cell line from the peripheral blood of a patient with psoriasis vulgaris. The newly established cells, termed YAP cells, grew in a suspension culture. In Wright-Giemsa-stained preparations, YAP cells were round or polygonal in shape. Transmission electron microscopy showed that the cells had clear nuclei with well-defined nucleoli. There were frequent mitochondria, a relatively abundant endoplasmic reticulum profile, free ribosomes and an occasional Golgi apparatus. Cytochemical studies showed a positive reaction for ~-naphthyl butyrate esterase, which was completely inhibited by sodium fluoride, a diffuse positive reaction for periodic acid-Schiff, and a negative result for alkaline phosphatase and peroxidase. A large population of YAP cells reacted with the CD4, CDllb, CD25 and CD33 surface markers, but not with CD2, CD3, CD8 or CD19. We also found that YAP cells produced considerable amounts of TNF~z, which was detected in the culture supernatant when the cells were treated with 1 ng/ml 12-O-tetradecanoylphorbol-13-acetate (TPA). Chromosome analyses showed that YAP cells contained a variety of marker chromosomes. It should be stressed that YAP cells were derived from a patient with a non-neoplastic disorder, whereas most monocytic cell lines previously reported are of malignant origin. This newly established cell line might be valuable for studying the pathogenesis of psoriasis, especially the role of monocytes/macrophages in the aetiology of the disease.

Acta Obstetricia et Gynecologica Scandinavica, 1990

Established cancer cell lines are widely used to study the biological features of malignant cells... more Established cancer cell lines are widely used to study the biological features of malignant cells. Permanent cell lines provide practically unlimited amounts of malignant cells derived from the same origin and allow the comparison of the results obtained from different experiments. In addition, the use of cell lines overcomes the concomitant technical difficulties, which are common when clinical specimens are used. The present study was undertaken to establish and characterize new cell lines derived from malignant tumors of the female genital tract and to evaluate the effects of an anti-estrogenic agent, tamoxifen (TAM) on endometrial, cervical, and vulvar carcinomas in vitro. Six well characterized, long-established cell lines derived from cervical (CasKi, HeLa, ME-180) and endometrial (HEC-1, KLE, RL95-2) carcinomas as well as four new cell lines established during this study were used for TAM-experiments. The ERpositive breast carcinoma cell line MCF-7 was used as control. New endometrial (UM-EC-1, UM-EC-2) and vulvar (UM-SCV-1 A, UM-SCV-1B) carcinoma cell lines were established using explant technique and characterization of the cells included testing their tumorigenity in nude mice as well as karyotype and cell surface antigen expression. All four cell lines produced nude mice tumors, which shared the histological features of the primary human tumors. Multiple metaphase cells at different passages were analysed to identify the consensus karyotype of the cell lines. Selected slides were G-banded, C-banded, Qbanded. or stained with the Ag-NOR (silver staining of the nucleolus-organizing region) technique. Cytogenetic analysis of the cell lines revealed varying

Archives of Pathology & Laboratory Medicine, 2000

Although inflammatory myofibroblastic tumors, originally described in the lung, have recently bee... more Although inflammatory myofibroblastic tumors, originally described in the lung, have recently been recognized to occur in various sites, their origin in bone is exceptional. We present a case of inflammatory myofibroblastic tumor, so-called inflammatory fibrosarcoma, of the bone. The tumor occurred in the iliac bone of a 70-year-old woman. Standard radiographs and magnetic resonance imaging revealed a destructive bone tumor that expanded into the soft tissue. Although the patient underwent excision of the hemi-iliac bone, multiple pulmonary metastases were noted 1 year after the operation. On histologic examination, the tumor was found to be composed of a sarcoma-like cellular area and a hypocellular fibrous area. Inflammatory cell infiltration into the tumor was a distinctive feature and is analogous with that of conventional inflammatory myofibroblastic tumor or inflammatory fibrosarcoma of the soft tissue. This is the first report to our knowledge of an inflammatory myofibroblast...

Background and purpose. Giant cell tumor (GCT) of the small bones (small-bone GCT) is usually rar... more Background and purpose. Giant cell tumor (GCT) of the small bones (small-bone GCT) is usually rare and considered somewhat different from conventional GCT. The purpose of this study was to investigate and report the clinicopathological features of 11 cases with small-bone GCT. Materials and methods. Patient information was obtained with the help of questionnaires. X-rays and paraffin blocks obtained from several institutions were clinically, radiographically, and histologically evaluated. Results. Small-bone GCT was observed in younger patients compared to conventional GCT; 5 of the 11 (45%) patients were below 20 years of age, whereas the corresponding figure for all GCT patients is 16 % in Japan. Excessive cortical bone expansion is a special feature. There were two cases of recurrence and one case of lung metastasis; the primary lesion was in the hand for all three cases. In contrast, no primary lesion of the foot recurred or metastasized. Varying degrees of positive p63 immunost...

PubMed, Aug 6, 2005

The aims of this study were to investigate the anti-tumor effects of consecutive low-dose cisplat... more The aims of this study were to investigate the anti-tumor effects of consecutive low-dose cisplatin (LD-CDDP) in comparison with single high-dose CDDP (HD-CDDP) in combination with caffeine. Materials and methods: Human fibrosarcoma cells (HT-1080) were transplanted in BALB/C-nu mice. According to the administration of CDDP and caffeine, 5 groups were defined: HD-CDDP, LD-CDDP, HD-CDDP +caffeine, LD-CDDP +caffeine and control group. The total dose of CDDP was 3.5 or 6 mg/kg. CDDP was injected i.p. bolus (HD-CDDP) or divided into 5 days (LD-CDDP). Caffeine was injected i.p. at a dose of 60 mg/kg twice a day for 4 days. Results: Significant inhibition of tumor growth and prolongation of survival time were recognized in the HD- and LD-CDDP groups with and without caffeine compared to the control group. The caffeine-assisted groups had no advantage compared to the CDDP alone groups. Conclusion: The effects of LD-CDDP were similar to the effect of HD-CDDP.

PubMed, Nov 11, 2006

Background: Undifferentiated pleomorphic sarcoma (UPS) is among the most common soft tissue sarco... more Background: Undifferentiated pleomorphic sarcoma (UPS) is among the most common soft tissue sarcomas in adults. In order to improve its aggressive course or prognosis and establish new therapeutic methods, molecular genetic and biological characterizations of UPS are required. Materials and methods: A new human UPS cell line (FPS-1) was established from UPS of the upper arm of a 79-year-old man. The cell line has been maintained for over 14 months with more than 60 passages. FPS-1 cells were characterized using molecular biological methods. Results: FPS-1 cells showed the same morphological and immunophenotypical characteristics as the primary tumor. Cytogenetic and molecular analyses revealed a nonsense mutation in exon 6 of the p53 gene. Epidermal growth factor receptor (EGFR) and cyclooxygenase-2 (COX-2) were expressed in FPS-1 cells. Conclusion: FPS-1 cells might be useful for investigating biological behavior and developing new molecular targeting antitumor drugs for UPS with EGFR or COX-2 expression.

Virchows Archiv, Nov 18, 2009

Malignant peripheral nerve sheath tumor (MPNST) is a rare soft tissue sarcoma. We established a n... more Malignant peripheral nerve sheath tumor (MPNST) is a rare soft tissue sarcoma. We established a new human MPNST cell line (designated FMS-1) from MPNST of the right brachial plexus of a 69-year-old woman with NF1. The cell line has been maintained for >24 months with >100 passages. FMS-1 cells showed a fibrosarcoma-like or epithelioid pattern in the heterotransplanted tumor, compared with a fascicular growth pattern of short-spindle tumor cells in the primary tumor. Immunophenotypically, FMS-1 cells showed almost the same characteristics as the primary tumor. Cytogenetic and molecular analyses revealed a deletion in exons 5-8 of the p53 gene. Epidermal growth factor receptor (EGFR) and cyclooxygenase (COX)-2 were expressed in FMS-1 cells. To improve the highly aggressive course and poor prognosis and establish new therapeutic methods, molecular genetic and biological characterizations of MPNST are required. Thus, FMS-1 cells might be useful for investigating biological behaviors and developing new molecular-targeting antitumor drugs for MPNST expressing EGFR or COX-2.

Medical Principles and Practice, Sep 22, 2012

Remitting seronegative symmetrical synovitis with pitting edema (RS3PE) syndrome is an uncommon d... more Remitting seronegative symmetrical synovitis with pitting edema (RS3PE) syndrome is an uncommon disorder, characterized by an acute-onset seronegative inflammation with pitting edema in the distal extremities, remitting without functional impairment. RS3PE syndrome is known as a paraneoplastic syndrome caused by some solid cancers and hematologic diseases. However, RS3PE syndrome rarely occurs due to inflammatory disease including infections and crystal-induced arthritis. We describe herein an extremely rare case of gouty arthritis-induced RS3PE syndrome in a 76-year-old man. Case Report The patient was a 76-year-old man with no remarkable past medical history including gout/hyperuricemia. He presented with a 1-month history of swelling and mild pain in the dorsum of his right foot, and a 3-week history of the same symptoms in his left foot. In addition he had a 1-week history of swelling and moderate pain in the dorsum of both of his hands with severe pain in his left wrist. There was no history of trauma. Upon examination, his body temperature was 38.1 ° C and local examination revealed swelling with pitting edema on the dorsum of his hands and feet bilaterally with surrounding erythema Key Words Remitting seronegative symmetrical synovitis with pitting edema syndrome ؒ Crystal-induced arthritis ؒ Gout ؒ Intra-articular corticosteroid injection ؒ Nonsteroidal anti-inflammatory drugs Abstract Objective: To describe a rare case of remitting seronegative symmetrical synovitis with pitting edema (RS3PE) syndrome caused by gouty arthritis. Clinical Presentation and Intervention: A 76-year-old man presented with swelling and pain in the dorsum of feet and hands bilaterally. From the laboratory and radiologic findings, the diagnosis of gout-induced RS3PE syndrome was made. Conservative therapy with nonsteroidal anti-inflammatory drugs (NSAIDs) and intra-articular corticosteroid injection in the wrist joint completely and rapidly resolved all symptoms. The patient was successfully treated with oral administration of NSAIDs and a one-time intra-articular corticosteroid injection in the left wrist joint. Conclusion: This case demonstrated the importance of considering the possibility of crystal-induced arthritis such as gout and pseudogout, as well as malignant disease, when diagnosing the primary disease responsible for RS3PE syndrome.

The American Journal of Surgical Pathology, Dec 1, 2001

ABSTRACT

Anticancer research, 2018

Clear cell sarcoma (CCS) of soft tissue is exceedingly rare and frequently exhibits aggressive be... more Clear cell sarcoma (CCS) of soft tissue is exceedingly rare and frequently exhibits aggressive behavior. Toward the goals of improving the aggressive course and poor prognosis of CCS, and establish new therapeutic methods, molecular genetic and biological characterizations of CCS are required. A new human CCS cell line (designated RSAR001) was established from the pleural effusion of a 44-year-old man with multiple lung metastases and pleural dissemination. The cell line and its xenograft were characterized including their morphology, immunohistochemistry, cytogenetic analysis, reverse transcription-polymerase chain reaction, direct sequencing analysis, and fluorescence in situ hybridization analysis. The cell line has been maintained for over 12 months with more than 50 passages. RSAR001 cells exhibited a fascicular or diffuse growth pattern of short spindle- or oval-shaped cells with clear cytoplasm in heterotransplanted tumor, that was similar to the primary tumor. Immunophenotyp...

Hellenic journal of nuclear medicine, 2016

We describe herein two tumor-induced osteomalacia (TIO) cases for whom the causative lesions, loc... more We describe herein two tumor-induced osteomalacia (TIO) cases for whom the causative lesions, located in their popliteal fossa, that were not identified in the standard field of fluorine-18-fluorodeoxyglucose positron emission tomography/computed tomography ((18)F-FDG PET/CT), which usually images only the head, trunk, and proximal parts of the extremities. A 47 years old Japanese man with multiple pathological fractures due to osteomalacia, accompanied by muscle weakness, hypophosphatemia, and an elevation of alkaline phosphatase (ALP) was referred to our hospital. A (18)F-FDG PET/CT scan was performed, but no (18)F-FDG uptake was detected in the standard field of imaging. Magnetic resonance imaging revealed a small subcutaneous tumor (1.9×1.2×0.6cm) of the left posteriomedial knee, displaying uniform enhancement on gadolinium-enhanced T1-weighted fat-suppression imaging. The tumor was resected widely and diagnosed as phosphaturic mesenchymal tumor, mixed connective tissue variant ...

Anticancer research

The aims of this study were to investigate the anti-tumor effects of consecutive low-dose cisplat... more The aims of this study were to investigate the anti-tumor effects of consecutive low-dose cisplatin (LD-CDDP) in comparison with single high-dose CDDP (HD-CDDP) in combination with caffeine. Human fibrosarcoma cells (HT-1080) were transplanted in BALB/C-nu mice. According to the administration of CDDP and caffeine, 5 groups were defined: HD-CDDP, LD-CDDP, HD-CDDP +caffeine, LD-CDDP +caffeine and control group. The total dose of CDDP was 3.5 or 6 mg/kg. CDDP was injected i.p. bolus (HD-CDDP) or divided into 5 days (LD-CDDP). Caffeine was injected i.p. at a dose of 60 mg/kg twice a day for 4 days. Significant inhibition of tumor growth and prolongation of survival time were recognized in the HD- and LD-CDDP groups with and without caffeine compared to the control group. The caffeine-assisted groups had no advantage compared to the CDDP alone groups. The effects of LD-CDDP were similar to the effect of HD-CDDP.

Ultrastructural Pathology, 2008

Fibronexus (fibronexus junction) has been thought to be a characteristic ultrastructural feature ... more Fibronexus (fibronexus junction) has been thought to be a characteristic ultrastructural feature of myofibroblasts, but it is controversial as to whether fibronexus is a characteristic of various myofibroblastic tumors. We report here a case of low-grade myofibrosarcoma with fibronexus arising in the right arm of an 80-year-old man. Histologically, the tumor was composed of relatively uniform and slender spindle cells arranged in fascicles. The nuclei with fusiform and tapered shapes were mildly hyperchromatic, but never exhibited pleomorphism. Mitotic figures were common, but no atypical mitosis was identified. At the tumor periphery, tumor cells had invaded into the surrounding skeletal muscle tissue. Tumor cells were positive diffusely for alpha-smooth muscle actin and less intensely for desmin, but were negative for h-caldesmon and S-100 protein. Ultrastructurally, tumor cells had well developed cytoplasmic organelles and varying amounts of peripheral or subplasmalemmal bundles of thin myofilaments with focal density. In addition, well formed, long fibronectin fibrils adjacent to the cell surface and fibronexus contacting intracellular myofilaments were easily identified. We believe that fibronexus is a useful ultrastructural feature for differentiating myofibrosarcoma from other myogenic sarcomas.

Histopathology, 2002

Extra-abdominal desmoid fibromatosis: two familial cases with synchronous and metachronous multic... more Extra-abdominal desmoid fibromatosis: two familial cases with synchronous and metachronous multicentric hyalinizing nodules Aims: Extra-abdominal desmoid fibromatosis is an uncommon tumour. We present here two exceptional familial cases of extra-abdominal desmoid fibromatosis, one of which was synchronous and metachronous. Methods and results: The first patient was a 37-year-old woman who had noted a tumour growing on the dorsum of her right foot when she was 12 years old. She underwent excision of the tumour but in the following year the tumour recurred locally and grew into multiple nodules. Subsequently, multicentric tumours appeared in her knee, distal and posterior aspects of her thigh, right back and right anterior shoulder. Polyostotic fibrous dysplasia of the femur and cranium was found on radiological examination. The second patient was a 74-year-old man, the uncle of the first patient. He underwent an excisional operation of a tumour on the internal malleolus surface of his fibula when he was 46 years old. The tumour recurred 7 years later and was excised. His post-operative course has been uneventful. The histology of the primary and recurrent tumours was distinctive and consistently showed hyalinizing scar-like features. Conclusions: Familial cases of extra-abdominal desmoid fibromatosis with extensive multicentric lesions and distinctive hyalinizing scar-like features are described. Recently, attenuated familial adenomatous polyposis with familial desmoid fibromatosis has been recognized, and familial desmoid fibromatosis without adenomatous polyposis may also be one of its variants. Although the present cases have no history of colon polyposis or carcinoma, monitoring of the intestinal tract would seem to be indicated.

Annals of Diagnostic Pathology, 2012

Spindle cell hemangioma, a rare benign tumor characterized by cavernous blood vessels and spindle... more Spindle cell hemangioma, a rare benign tumor characterized by cavernous blood vessels and spindled areas, typically arises in the subcutis of the distal extremities, particularly the hand. The case of intraosseous spindle cell hemangioma is extremely rare, and only 1 case arises in the frontal bone has been reported previously. We describe herein a case of intraosseous spindle cell hemangioma occurring in the left calcaneus in a 65-year-old woman. The patient was successfully treated by the operation. The present case is instructive especially in the differential diagnosis of primary bone tumor structured by spindle cells, for which the possibility of spindle cell hemangioma should be considered.

Australasian Journal of Dermatology

Scientific Reports, 2019

Hepatitis C virus (HCV) cell culture systems have facilitated the development of efficient direct... more Hepatitis C virus (HCV) cell culture systems have facilitated the development of efficient direct-acting antivirals against HCV. Huh-7.5, a subline of the human hepatoma cell line Huh-7, has been used widely to amplify HCV because HCV can efficiently replicate in these cells due to a defect in innate antiviral signalling. Recently, we established a novel cell line, KH, derived from human hepatocellular carcinoma, which showed atypical uptake of gadolinium ethoxybenzyl diethylenetriamine pentaacetic acid (Gd-EOB-DTPA) in a Gd-EOB-DTPA-enhanced magnetic resonance imaging study. KH cells expressed hepatocyte markers including microRNA-122 (miR-122) at a lower level than Huh-7.5 cells. We demonstrated that KH cells could support the entire life cycle of HCV; however, HCV replicated at a lower rate in KH cells compared to Huh-7.5 cells, and virus particles produced from KH cells seemed to have some disadvantages in viral assembly compared with those produced from Huh-7.5 cells. KH cells had more robust interferon-stimulated gene expression and induction upon HCV RNA transfection, interferon-α2b addition, and HCV infection than Huh-7.5 cells. Interestingly, both miR-122 supplementation and IRF3 knockout in KH cells boosted HCV replication to a similar level as in Huh-7.5 cells, suggesting that intact innate antiviral signalling and lower miR-122 expression limit HCV replication in KH cells. KH cells will enable a deeper understanding of the role of the innate immune response in persistent HCV infection.

Archives of Dermatological Research, 1996

Human monocyte/macrophage lineages have unique phagocytic and immune-regulatory functions. We est... more Human monocyte/macrophage lineages have unique phagocytic and immune-regulatory functions. We established a promonocytic cell line from the peripheral blood of a patient with psoriasis vulgaris. The newly established cells, termed YAP cells, grew in a suspension culture. In Wright-Giemsa-stained preparations, YAP cells were round or polygonal in shape. Transmission electron microscopy showed that the cells had clear nuclei with well-defined nucleoli. There were frequent mitochondria, a relatively abundant endoplasmic reticulum profile, free ribosomes and an occasional Golgi apparatus. Cytochemical studies showed a positive reaction for ~-naphthyl butyrate esterase, which was completely inhibited by sodium fluoride, a diffuse positive reaction for periodic acid-Schiff, and a negative result for alkaline phosphatase and peroxidase. A large population of YAP cells reacted with the CD4, CDllb, CD25 and CD33 surface markers, but not with CD2, CD3, CD8 or CD19. We also found that YAP cells produced considerable amounts of TNF~z, which was detected in the culture supernatant when the cells were treated with 1 ng/ml 12-O-tetradecanoylphorbol-13-acetate (TPA). Chromosome analyses showed that YAP cells contained a variety of marker chromosomes. It should be stressed that YAP cells were derived from a patient with a non-neoplastic disorder, whereas most monocytic cell lines previously reported are of malignant origin. This newly established cell line might be valuable for studying the pathogenesis of psoriasis, especially the role of monocytes/macrophages in the aetiology of the disease.

Acta Obstetricia et Gynecologica Scandinavica, 1990

Established cancer cell lines are widely used to study the biological features of malignant cells... more Established cancer cell lines are widely used to study the biological features of malignant cells. Permanent cell lines provide practically unlimited amounts of malignant cells derived from the same origin and allow the comparison of the results obtained from different experiments. In addition, the use of cell lines overcomes the concomitant technical difficulties, which are common when clinical specimens are used. The present study was undertaken to establish and characterize new cell lines derived from malignant tumors of the female genital tract and to evaluate the effects of an anti-estrogenic agent, tamoxifen (TAM) on endometrial, cervical, and vulvar carcinomas in vitro. Six well characterized, long-established cell lines derived from cervical (CasKi, HeLa, ME-180) and endometrial (HEC-1, KLE, RL95-2) carcinomas as well as four new cell lines established during this study were used for TAM-experiments. The ERpositive breast carcinoma cell line MCF-7 was used as control. New endometrial (UM-EC-1, UM-EC-2) and vulvar (UM-SCV-1 A, UM-SCV-1B) carcinoma cell lines were established using explant technique and characterization of the cells included testing their tumorigenity in nude mice as well as karyotype and cell surface antigen expression. All four cell lines produced nude mice tumors, which shared the histological features of the primary human tumors. Multiple metaphase cells at different passages were analysed to identify the consensus karyotype of the cell lines. Selected slides were G-banded, C-banded, Qbanded. or stained with the Ag-NOR (silver staining of the nucleolus-organizing region) technique. Cytogenetic analysis of the cell lines revealed varying

Archives of Pathology & Laboratory Medicine, 2000

Although inflammatory myofibroblastic tumors, originally described in the lung, have recently bee... more Although inflammatory myofibroblastic tumors, originally described in the lung, have recently been recognized to occur in various sites, their origin in bone is exceptional. We present a case of inflammatory myofibroblastic tumor, so-called inflammatory fibrosarcoma, of the bone. The tumor occurred in the iliac bone of a 70-year-old woman. Standard radiographs and magnetic resonance imaging revealed a destructive bone tumor that expanded into the soft tissue. Although the patient underwent excision of the hemi-iliac bone, multiple pulmonary metastases were noted 1 year after the operation. On histologic examination, the tumor was found to be composed of a sarcoma-like cellular area and a hypocellular fibrous area. Inflammatory cell infiltration into the tumor was a distinctive feature and is analogous with that of conventional inflammatory myofibroblastic tumor or inflammatory fibrosarcoma of the soft tissue. This is the first report to our knowledge of an inflammatory myofibroblast...