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Papers by Talkad S Muralidhar

Research paper thumbnail of Effect of Tyrosine ammonia lyase from Plectranthus amboinicus plant extract for the treatment of Tyrosimemia II and molecular docking for potent activators

Tyrosine ammonia lyase TAL (EC 4.3.1.23) is an enzyme required for transforming L – Tyrosine into... more Tyrosine ammonia lyase TAL (EC 4.3.1.23) is an enzyme required for transforming L – Tyrosine into p – Coumaric acid. The objective of the experiment is to extract TAL from Plectranthus amboinicus and to increase its activity. This enzyme can also be used in the treatment of metabolic disorder Tyrosinemia II where abnormal accumulation of Tyrosine occurs in the body. Tyrosine aminotransferase is basically involved in the treatment of Tyrosinemia in humans. A large number of phenylpropanoids are generated from p-coumaric acid, which is a derivative of the primary metabolite, phenylalanine. The first two steps in the phenylpropanoid biosynthetic pathway are catalyzed by phenylalanine ammonia-lyase and cinnamate 4-hydroxylase, and the coupling of these two enzymes forms a rate-limiting step in the pathway. For the generation of p-coumaric acid, the conversion from phenylalanine to p-coumaric acid that is catalyzed by two enzymes can be theoretically performed by a single enzyme, tyrosin...

Research paper thumbnail of Induced Mutational Studies on Saccharomyces Cerevisiae for Bioethanol Production from Fruit Waste

Rising petroleum costs, trade imbalances and environmental concerns have stimulated efforts to an... more Rising petroleum costs, trade imbalances and environmental concerns have stimulated efforts to an advance in the microbial production of fuels from lignocellulosic biomass. Ethanol, the most widely used renewable liquid transportation fuel, has only 70 % of the energy content of gasoline and its hygroscopicity makes it incompatible with existing fuel, storage and distribution infrastructure. Saccharomyces cerevisiae is the only yeast that can rapidly grow under aerobic as well as in anaerobic conditions. This unique ability plays a major role in various industrial applications of S. cerevisiae, including beer fermentation, wine fermentation and large-scale production of biofuel ethanol. Here we focused on Induced Mutations for Yeast to attain better Bioethanol yield from Fruit waste. We planned for other substrates such as the fruit waste example banana peel, chikko peel, mango peel, pine apple peel and other fruit waste. Ethanol estimated by chromic acid assay, Induction of mutation by EMS &UV. Ethanol estimated by Gas chromatography, Isolation of genomic DNA using mini prep filters. The Results obtained from the Gas Chromatography for the Saccharomyces cerevisiae-EMS & UV Mutated, the Purity level for the Ethanol was found to be 73.5 % & 53.9 % respectively, with acid treatment it showed 53.9 %.The Genomic DNA bands of S. cerevisiae (control) and mutated (UV & EMS) has shown slight variation since, it could be responsible for the higher ethanol production, this method can be utilized for renewable source of energy & domestic wastes with sustainable technology.

Research paper thumbnail of Effect of D-400, an Ayurvedic Herbal Formulation on Experimentally Induced Diabetes Mellitus

Phytotherapy Research, 1996

Research paper thumbnail of Experimental Assessment of Relative Efficacy of Drugs of Herbal Origin on Sexual Performance and Hormone Levels in Alcohol Exposed and Normal Rats

Phytotherapy Research - PHYTOTHER RES, 1996

Alcohol can affect the hypothalamo-pituitary-gonadal axis leading to impotence and sterility by v... more Alcohol can affect the hypothalamo-pituitary-gonadal axis leading to impotence and sterility by various mechanisms of action. In this study evaluation of the effect of three herbomineral formulations, alone and as combinations was done in alcohol exposed and normal rats. The preparations used showed beneficial effects of various degrees with respect to the sexual behaviour of animals. There was also improvement in the number of LH-FSH producing basophil cells in the pituitary and raised levels of circulating testosterone. The mean sperm count was also higher in the drug treated animals.

Research paper thumbnail of Effect of D-400, a herbomineral preparation on lipid profile, glycated haemoglobin and glucose tolerance in streptozotocin induced diabetes in rats

Research paper thumbnail of A Safety Evaluation of Starchytaperta Indica, a Potent Traditional Anti-oxidant Medicinal Plant

European Journal of …, 2010

European Journal of Scientific Research ISSN 1450-216X Vol.46 No.1 (2010), pp.06-017 © EuroJourna... more European Journal of Scientific Research ISSN 1450-216X Vol.46 No.1 (2010), pp.06-017 © EuroJournals Publishing, Inc. 2010 http://www.eurojournals.com/ejsr.htm ... A Safety Evaluation of Starchytaperta Indica, a Potent ... Zuhaib Zafar Al Ameen College of Pharmacy, Hosur Road, Near ...

Research paper thumbnail of Protective Effect of Salacia Oblanga and Quercetin on Cyclophosphamide-Induced Chromosome Aberrations in Rat Bone Marrow Cells

Research paper thumbnail of Effect of a herbomineral preparation D-400 in streptozotocin-induced diabetic rats

Journal of Ethnopharmacology, 1996

This study was undertaken to investigate the effect of D-400, a herbomineral formulation on blood... more This study was undertaken to investigate the effect of D-400, a herbomineral formulation on blood sugar and other biochemical parameters in streptozotocin-induced diabetic rats. There was significant reduction in blood urea nitrogen, serum creatinine and serum uric acid levels in the D-400-treated group and considerable lowering of AUC in oral glucose tolerance test (OGTT). The D-400-treated group showed significant lowering of triglycerides and HDL cholesterol. There was a rise in hepatic glycogen level close to normal after D-400 treatment. In the pancreas of diabetic rats. D-400 therapy showed significant increase in islet number and beta cell count and appeared to bring about blood sugar homoeostasis by increasing insulin secretion and repair/regeneration of endocrine pancreas.

Research paper thumbnail of Effect of D-400, a herbomineral preparation on lipid profile, glycated haemoglobin and glucose tolerance in streptozotocin induced diabetes in rats

Research paper thumbnail of Effect of Tyrosine ammonia lyase from Plectranthus amboinicus plant extract for the treatment of Tyrosimemia II and molecular docking for potent activators

Tyrosine ammonia lyase TAL (EC 4.3.1.23) is an enzyme required for transforming L – Tyrosine into... more Tyrosine ammonia lyase TAL (EC 4.3.1.23) is an enzyme required for transforming L – Tyrosine into p – Coumaric acid. The objective of the experiment is to extract TAL from Plectranthus amboinicus and to increase its activity. This enzyme can also be used in the treatment of metabolic disorder Tyrosinemia II where abnormal accumulation of Tyrosine occurs in the body. Tyrosine aminotransferase is basically involved in the treatment of Tyrosinemia in humans. A large number of phenylpropanoids are generated from p-coumaric acid, which is a derivative of the primary metabolite, phenylalanine. The first two steps in the phenylpropanoid biosynthetic pathway are catalyzed by phenylalanine ammonia-lyase and cinnamate 4-hydroxylase, and the coupling of these two enzymes forms a rate-limiting step in the pathway. For the generation of p-coumaric acid, the conversion from phenylalanine to p-coumaric acid that is catalyzed by two enzymes can be theoretically performed by a single enzyme, tyrosin...

Research paper thumbnail of Induced Mutational Studies on Saccharomyces Cerevisiae for Bioethanol Production from Fruit Waste

Rising petroleum costs, trade imbalances and environmental concerns have stimulated efforts to an... more Rising petroleum costs, trade imbalances and environmental concerns have stimulated efforts to an advance in the microbial production of fuels from lignocellulosic biomass. Ethanol, the most widely used renewable liquid transportation fuel, has only 70 % of the energy content of gasoline and its hygroscopicity makes it incompatible with existing fuel, storage and distribution infrastructure. Saccharomyces cerevisiae is the only yeast that can rapidly grow under aerobic as well as in anaerobic conditions. This unique ability plays a major role in various industrial applications of S. cerevisiae, including beer fermentation, wine fermentation and large-scale production of biofuel ethanol. Here we focused on Induced Mutations for Yeast to attain better Bioethanol yield from Fruit waste. We planned for other substrates such as the fruit waste example banana peel, chikko peel, mango peel, pine apple peel and other fruit waste. Ethanol estimated by chromic acid assay, Induction of mutation by EMS &UV. Ethanol estimated by Gas chromatography, Isolation of genomic DNA using mini prep filters. The Results obtained from the Gas Chromatography for the Saccharomyces cerevisiae-EMS & UV Mutated, the Purity level for the Ethanol was found to be 73.5 % & 53.9 % respectively, with acid treatment it showed 53.9 %.The Genomic DNA bands of S. cerevisiae (control) and mutated (UV & EMS) has shown slight variation since, it could be responsible for the higher ethanol production, this method can be utilized for renewable source of energy & domestic wastes with sustainable technology.

Research paper thumbnail of Effect of D-400, an Ayurvedic Herbal Formulation on Experimentally Induced Diabetes Mellitus

Phytotherapy Research, 1996

Research paper thumbnail of Experimental Assessment of Relative Efficacy of Drugs of Herbal Origin on Sexual Performance and Hormone Levels in Alcohol Exposed and Normal Rats

Phytotherapy Research - PHYTOTHER RES, 1996

Alcohol can affect the hypothalamo-pituitary-gonadal axis leading to impotence and sterility by v... more Alcohol can affect the hypothalamo-pituitary-gonadal axis leading to impotence and sterility by various mechanisms of action. In this study evaluation of the effect of three herbomineral formulations, alone and as combinations was done in alcohol exposed and normal rats. The preparations used showed beneficial effects of various degrees with respect to the sexual behaviour of animals. There was also improvement in the number of LH-FSH producing basophil cells in the pituitary and raised levels of circulating testosterone. The mean sperm count was also higher in the drug treated animals.

Research paper thumbnail of Effect of D-400, a herbomineral preparation on lipid profile, glycated haemoglobin and glucose tolerance in streptozotocin induced diabetes in rats

Research paper thumbnail of A Safety Evaluation of Starchytaperta Indica, a Potent Traditional Anti-oxidant Medicinal Plant

European Journal of …, 2010

European Journal of Scientific Research ISSN 1450-216X Vol.46 No.1 (2010), pp.06-017 © EuroJourna... more European Journal of Scientific Research ISSN 1450-216X Vol.46 No.1 (2010), pp.06-017 © EuroJournals Publishing, Inc. 2010 http://www.eurojournals.com/ejsr.htm ... A Safety Evaluation of Starchytaperta Indica, a Potent ... Zuhaib Zafar Al Ameen College of Pharmacy, Hosur Road, Near ...

Research paper thumbnail of Protective Effect of Salacia Oblanga and Quercetin on Cyclophosphamide-Induced Chromosome Aberrations in Rat Bone Marrow Cells

Research paper thumbnail of Effect of a herbomineral preparation D-400 in streptozotocin-induced diabetic rats

Journal of Ethnopharmacology, 1996

This study was undertaken to investigate the effect of D-400, a herbomineral formulation on blood... more This study was undertaken to investigate the effect of D-400, a herbomineral formulation on blood sugar and other biochemical parameters in streptozotocin-induced diabetic rats. There was significant reduction in blood urea nitrogen, serum creatinine and serum uric acid levels in the D-400-treated group and considerable lowering of AUC in oral glucose tolerance test (OGTT). The D-400-treated group showed significant lowering of triglycerides and HDL cholesterol. There was a rise in hepatic glycogen level close to normal after D-400 treatment. In the pancreas of diabetic rats. D-400 therapy showed significant increase in islet number and beta cell count and appeared to bring about blood sugar homoeostasis by increasing insulin secretion and repair/regeneration of endocrine pancreas.

Research paper thumbnail of Effect of D-400, a herbomineral preparation on lipid profile, glycated haemoglobin and glucose tolerance in streptozotocin induced diabetes in rats