Tamara Fucase - Academia.edu (original) (raw)

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Papers by Tamara Fucase

Research paper thumbnail of Purificação e caracterização de inibidores de proteases de veneno de <i>Bitis gabonica rhinoceros</i> com potencial farmacológico

Agradeço a Deus pelo dom da vida e toda a proteção no decorrer do caminho. Ao meu orientador e qu... more Agradeço a Deus pelo dom da vida e toda a proteção no decorrer do caminho. Ao meu orientador e querido amigo Dr. Patrick Jack Spencer pelo apoio e confiança. Ao meu amigo e ex-colega de trabalho Dr.Vincent Louis Viala sempre me apoiando em todos os experimentos. Aos amigos e companheiros de bancada Marcela Di Giacomo Messias e Samuel Levindo por todo carinho, ajuda, paciência e confiança Ao querido amigo Rodolfo Ferreira e minha amiguinha Larissa Miranda por todo apoio e conselhos

Research paper thumbnail of Isolation and biochemical characterization of bradykinin-potentiating peptides from Bitis gabonica rhinoceros

The journal of venomous animals and toxins including tropical diseases, 2017

Venoms represent a still underexplored reservoir of bioactive components that might mitigate or c... more Venoms represent a still underexplored reservoir of bioactive components that might mitigate or cure diseases in conditions in which conventional therapy is ineffective. The bradykinin-potentiating peptides (BPPs) comprise a class of angiotensin-I converting enzyme (ACE) inhibitors. The BPPs usually consist of oligopeptides with 5 to 13 residues with a high number of proline residues and the tripeptide Ile-Pro-Pro (IPP-tripeptide) in the C-terminus region and have a conserved N-terminal pyroglutamate residue. As a whole, the action of the BPPs on prey and snakebite victims results in the decrease of the blood pressure. The aim of this work was to isolate and characterize novel BPPs from the venom of Bitis gabonica rhinoceros. The crude venom of B. g. rhinoceros was fractionated by size exclusion chromatography and the peptide fraction (<7 kDa) was separated by reverse phase chromatography (RP-HPLC) and analyzed by ESI-IT-TOF-MS/MS. One new BPP was identified, synthetized and assa...

Research paper thumbnail of Estudo do veneno de crotalus vegrandis e de suas frações com atividade antitumoral

Research paper thumbnail of Proteomic analysis of the rare Uracoan rattlesnake Crotalus vegrandis venom: evidence of a broad arsenal of toxins

Toxicon : official journal of the International Society on Toxinology, Jan 21, 2015

The investigation of venoms has many clinical, pharmacological, ecological and evolutionary outco... more The investigation of venoms has many clinical, pharmacological, ecological and evolutionary outcomes. The Crotalus spp. venom can cause hemorrhage, neurotoxicity, myotoxicity, coagulopathy and hypotension. Although neurotoxicity and hemorrhage usually does not occur for the same species, the rare Venezuelan species Crotalus vegrandis presents both characteristic. Different from the other species it has a restricted ecological niche and geographical distribution. Nevertheless, it has a raising medical importance as this rattlesnake population is increasing. Few works describe its neurotoxic and hemorrhagic features, but other toxins might play an important role in envenomation. We combined proteomic methods to identify for the first time the main components of it venom: 2D SDS-PAGE and gel-filtration chromatography for protein mixture decomplexation; LC-MS(2) of low molecular mass fractions and tryptic peptides; bioinformatic identification of toxin families and specific protein spec...

Research paper thumbnail of Venomics of the Australian eastern brown snake (Pseudonaja textilis): Detection of new venom proteins and splicing variants

Toxicon, 2015

Venomics of the Australian eastern brown snake (Pseudonaja textilis): detection of new venom prot... more Venomics of the Australian eastern brown snake (Pseudonaja textilis): detection of new venom proteins and splicing variants.

Research paper thumbnail of Purificação e caracterização de inibidores de proteases de veneno de <i>Bitis gabonica rhinoceros</i> com potencial farmacológico

Agradeço a Deus pelo dom da vida e toda a proteção no decorrer do caminho. Ao meu orientador e qu... more Agradeço a Deus pelo dom da vida e toda a proteção no decorrer do caminho. Ao meu orientador e querido amigo Dr. Patrick Jack Spencer pelo apoio e confiança. Ao meu amigo e ex-colega de trabalho Dr.Vincent Louis Viala sempre me apoiando em todos os experimentos. Aos amigos e companheiros de bancada Marcela Di Giacomo Messias e Samuel Levindo por todo carinho, ajuda, paciência e confiança Ao querido amigo Rodolfo Ferreira e minha amiguinha Larissa Miranda por todo apoio e conselhos

Research paper thumbnail of Isolation and biochemical characterization of bradykinin-potentiating peptides from Bitis gabonica rhinoceros

The journal of venomous animals and toxins including tropical diseases, 2017

Venoms represent a still underexplored reservoir of bioactive components that might mitigate or c... more Venoms represent a still underexplored reservoir of bioactive components that might mitigate or cure diseases in conditions in which conventional therapy is ineffective. The bradykinin-potentiating peptides (BPPs) comprise a class of angiotensin-I converting enzyme (ACE) inhibitors. The BPPs usually consist of oligopeptides with 5 to 13 residues with a high number of proline residues and the tripeptide Ile-Pro-Pro (IPP-tripeptide) in the C-terminus region and have a conserved N-terminal pyroglutamate residue. As a whole, the action of the BPPs on prey and snakebite victims results in the decrease of the blood pressure. The aim of this work was to isolate and characterize novel BPPs from the venom of Bitis gabonica rhinoceros. The crude venom of B. g. rhinoceros was fractionated by size exclusion chromatography and the peptide fraction (<7 kDa) was separated by reverse phase chromatography (RP-HPLC) and analyzed by ESI-IT-TOF-MS/MS. One new BPP was identified, synthetized and assa...

Research paper thumbnail of Estudo do veneno de crotalus vegrandis e de suas frações com atividade antitumoral

Research paper thumbnail of Proteomic analysis of the rare Uracoan rattlesnake Crotalus vegrandis venom: evidence of a broad arsenal of toxins

Toxicon : official journal of the International Society on Toxinology, Jan 21, 2015

The investigation of venoms has many clinical, pharmacological, ecological and evolutionary outco... more The investigation of venoms has many clinical, pharmacological, ecological and evolutionary outcomes. The Crotalus spp. venom can cause hemorrhage, neurotoxicity, myotoxicity, coagulopathy and hypotension. Although neurotoxicity and hemorrhage usually does not occur for the same species, the rare Venezuelan species Crotalus vegrandis presents both characteristic. Different from the other species it has a restricted ecological niche and geographical distribution. Nevertheless, it has a raising medical importance as this rattlesnake population is increasing. Few works describe its neurotoxic and hemorrhagic features, but other toxins might play an important role in envenomation. We combined proteomic methods to identify for the first time the main components of it venom: 2D SDS-PAGE and gel-filtration chromatography for protein mixture decomplexation; LC-MS(2) of low molecular mass fractions and tryptic peptides; bioinformatic identification of toxin families and specific protein spec...

Research paper thumbnail of Venomics of the Australian eastern brown snake (Pseudonaja textilis): Detection of new venom proteins and splicing variants

Toxicon, 2015

Venomics of the Australian eastern brown snake (Pseudonaja textilis): detection of new venom prot... more Venomics of the Australian eastern brown snake (Pseudonaja textilis): detection of new venom proteins and splicing variants.

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