Tamara Karpunina - Academia.edu (original) (raw)

Papers by Tamara Karpunina

Research paper thumbnail of Interactions of Pseudomonas aeruginosa in predominant biofilm or planktonic forms of existence in mixed culture with Escherichia coli in vitro

Canadian Journal of Microbiology, 2013

Pseudomonas aeruginosa and Escherichia coli are known to be involved in mixed communities in dive... more Pseudomonas aeruginosa and Escherichia coli are known to be involved in mixed communities in diverse niches. In this study we examined the influence of the predominant form of cell existence of and the exometabolite production by P. aeruginosa strains on interspecies interactions, in vitro. Bacterial numbers of P. aeruginosa and E. coli in mixed plankton cultures and biofilms compared with their numbers in single plankton cultures and biofilms changed in a different way, but were in accordance with the form of P. aeruginosa cell existence. The mass of a mixed-species biofilm was greater than the mass of a single-species biofilm. Among the mixed biofilms, the one with the “planktonic” P. aeruginosa strain had the least biomass. The total pyocyanin and pyoverdin levels were found to be lower in all mixed plankton cultures. Despite this, clinical P. aeruginosa strains irrespective of the predominant form of existence (“biofilm” or “planktonic”) had a higher total concentration of exome...

Research paper thumbnail of Functional activity of Lactobacillus spp. isolated from the genital tract under the unfluence of putrescine and cadaverine

Вестник Пермского университета. Серия «Биология»=Bulletin of Perm University. Biology

Functional activity of Lactobacillus spp. was evatuated under the influence of bacterial diamines... more Functional activity of Lactobacillus spp. was evatuated under the influence of bacterial diamines. The dose-dependent effect of putrescine on growth characteristics of clinical strains has been established. Cadaverine at a concentration of 1 mmol/l in the culture medium significantly stimulated the accumulation of biomass of the tested strains. The results obtained confirm the destabilizing effect of bacterial amines agents on vaginal Lactobacillus spp. and thus their important direct role in weakening the biological protection of the vaginal microenvironment.

Research paper thumbnail of Enhanced hydroxyl radical generation by human leukocytes exposed to bacterial diamines highlighting immunomodulatory effect of microbial metabolites

Russian Journal of Infection and Immunity

Recently, there have been increasing an interest to study a role of polyamines in intercellular i... more Recently, there have been increasing an interest to study a role of polyamines in intercellular interactions, especially in the focus of inflammation, where accumulation of such polycations is observed. In this regard, products of microbial origin cadaverine and putrescine are of particular attention. The role of polyamines as scavengers of free radicals has been described, but no data of their effect on the leukocyte radical-producing activity have been obtained so far. The aim of the investigation was to study features of hydroxyl radical generation by human leukocytes exposed to microbe-derived polyamines. Materials and methods. Peripheral venous blood samples were obtained from 20 healthy donors. To assess radical production, a luminol-dependent chemiluminescence reaction was carried out with blood leukocytes pre-incubated with cadaverine (0.01 M) and putrescine (0.01 M), measured on a Luminoskan Ascent Thermo Labsystems luminometer (USA) for 180 min. For statistical analysis, a...

Research paper thumbnail of Peculiarities of Intermicrobial Relationships in the Vaginal Microbiota of Infertile Women

Research paper thumbnail of Features of inter-microbial relations in the infer tile women’s vagina microbiota

Medical news of the North Caucasus, 2019

Endometrial curettage in abnormal uterine bleeding and efficacy of progestins for control in case... more Endometrial curettage in abnormal uterine bleeding and efficacy of progestins for control in cases of hyperplasia.

Research paper thumbnail of Endogenic Intoxication Evaluation in Subfertile Women with Chronic Endometritis

The Bulletin of Contemporary Clinical Medicine, 2018

ОРИГИНАЛЬНЫЕ ИССЛЕДОВАНИЯ Прозрачность исследования. Исследование не имело спонсорской поддержки.... more ОРИГИНАЛЬНЫЕ ИССЛЕДОВАНИЯ Прозрачность исследования. Исследование не имело спонсорской поддержки. Авторы несут полную ответственность за предоставление окончательной версии рукописи в печать. Декларация о финансовых и других взаимоотношениях. Все авторы принимали участие в разработке концепции, дизайна исследования и в написании рукописи. Окончательная версия рукописи была одобрена всеми авторами. Авторы не получали гонорар за исследование.

Research paper thumbnail of Interactions of Pseudomonas aeruginosa in predominant biofilm or planktonic forms of existence in mixed culture with Escherichia coli in vitro

Canadian Journal of Microbiology, 2013

Pseudomonas aeruginosa and Escherichia coli are known to be involved in mixed communities in dive... more Pseudomonas aeruginosa and Escherichia coli are known to be involved in mixed communities in diverse niches. In this study we examined the influence of the predominant form of cell existence of and the exometabolite production by P. aeruginosa strains on interspecies interactions, in vitro. Bacterial numbers of P. aeruginosa and E. coli in mixed plankton cultures and biofilms compared with their numbers in single plankton cultures and biofilms changed in a different way, but were in accordance with the form of P. aeruginosa cell existence. The mass of a mixed-species biofilm was greater than the mass of a single-species biofilm. Among the mixed biofilms, the one with the “planktonic” P. aeruginosa strain had the least biomass. The total pyocyanin and pyoverdin levels were found to be lower in all mixed plankton cultures. Despite this, clinical P. aeruginosa strains irrespective of the predominant form of existence (“biofilm” or “planktonic”) had a higher total concentration of exome...

Research paper thumbnail of Functional activity of Lactobacillus spp. isolated from the genital tract under the unfluence of putrescine and cadaverine

Вестник Пермского университета. Серия «Биология»=Bulletin of Perm University. Biology

Functional activity of Lactobacillus spp. was evatuated under the influence of bacterial diamines... more Functional activity of Lactobacillus spp. was evatuated under the influence of bacterial diamines. The dose-dependent effect of putrescine on growth characteristics of clinical strains has been established. Cadaverine at a concentration of 1 mmol/l in the culture medium significantly stimulated the accumulation of biomass of the tested strains. The results obtained confirm the destabilizing effect of bacterial amines agents on vaginal Lactobacillus spp. and thus their important direct role in weakening the biological protection of the vaginal microenvironment.

Research paper thumbnail of Enhanced hydroxyl radical generation by human leukocytes exposed to bacterial diamines highlighting immunomodulatory effect of microbial metabolites

Russian Journal of Infection and Immunity

Recently, there have been increasing an interest to study a role of polyamines in intercellular i... more Recently, there have been increasing an interest to study a role of polyamines in intercellular interactions, especially in the focus of inflammation, where accumulation of such polycations is observed. In this regard, products of microbial origin cadaverine and putrescine are of particular attention. The role of polyamines as scavengers of free radicals has been described, but no data of their effect on the leukocyte radical-producing activity have been obtained so far. The aim of the investigation was to study features of hydroxyl radical generation by human leukocytes exposed to microbe-derived polyamines. Materials and methods. Peripheral venous blood samples were obtained from 20 healthy donors. To assess radical production, a luminol-dependent chemiluminescence reaction was carried out with blood leukocytes pre-incubated with cadaverine (0.01 M) and putrescine (0.01 M), measured on a Luminoskan Ascent Thermo Labsystems luminometer (USA) for 180 min. For statistical analysis, a...

Research paper thumbnail of Peculiarities of Intermicrobial Relationships in the Vaginal Microbiota of Infertile Women

Research paper thumbnail of Features of inter-microbial relations in the infer tile women’s vagina microbiota

Medical news of the North Caucasus, 2019

Endometrial curettage in abnormal uterine bleeding and efficacy of progestins for control in case... more Endometrial curettage in abnormal uterine bleeding and efficacy of progestins for control in cases of hyperplasia.

Research paper thumbnail of Endogenic Intoxication Evaluation in Subfertile Women with Chronic Endometritis

The Bulletin of Contemporary Clinical Medicine, 2018

ОРИГИНАЛЬНЫЕ ИССЛЕДОВАНИЯ Прозрачность исследования. Исследование не имело спонсорской поддержки.... more ОРИГИНАЛЬНЫЕ ИССЛЕДОВАНИЯ Прозрачность исследования. Исследование не имело спонсорской поддержки. Авторы несут полную ответственность за предоставление окончательной версии рукописи в печать. Декларация о финансовых и других взаимоотношениях. Все авторы принимали участие в разработке концепции, дизайна исследования и в написании рукописи. Окончательная версия рукописи была одобрена всеми авторами. Авторы не получали гонорар за исследование.