Tania Singh - Academia.edu (original) (raw)

Papers by Tania Singh

Research paper thumbnail of Comparison of two WHO partographs: a one year randomized controlled trial

Journal of the Turkish German Gynecological Association, 2011

To compare two World Health Organization (WHO) partographs - a composite partograph including the... more To compare two World Health Organization (WHO) partographs - a composite partograph including the latent phase with a simplified one without the latent phase in women with uncomplicated pregnancy. This was a randomized controlled trial conducted at a tertiary hospital at Belgaum, India. 743 women with term, singleton, vertex gestation, in spontaneous labor were included in the study over a period of one year. Either of the partographs was used on laboring women. The following outcomes were compared: labor crossing the alert and action line, augmentation of labor, rate of cesarean section, perinatal outcome, user friendliness and maternal complications. Statistical analysis was done using Chi-square test. Labor values crossed the alert and action lines significantly more often when composite partograph was used (p<0.001) in each, with increased number of augmentations (p<0.001). The number of vaginal deliveries were high (p<0.005) in the simplified group. There was no significant difference in the rate of cesarean deliveries due to non progress of labor in both groups (p=0.68). NICU admissions were higher in the composite group (p=0.035). Most resident doctors (93%) experienced difficulty with the composite partograph, but no resident doctor reported difficulty with the simplified partograph. The mean SD user friendliness score was lower for the composite partograph (2.87±1.86 vs 10.67±1.61; p<0.005). The WHO simplified partograph is easier to use and is a better option for both the laboring women and the user, when compared to composite partograph.

Research paper thumbnail of Echocardiographic and Clinical Correlates of Specific Early Repolarization Subtypes on Electrocardiogram

Journal of the American College of Cardiology, 2012

Research paper thumbnail of Chapter-092 Operative Site Infections

Ward Rounds in Obstetrics & Neonatology, 2016

Research paper thumbnail of Chapter-093 Blood Transfusion and Blood Components

Ward Rounds in Obstetrics & Neonatology, 2016

Research paper thumbnail of Chapter-017 Rh Negative Pregnancy

Ward Rounds in Obstetrics & Neonatology, 2016

Research paper thumbnail of Chapter-029 Hypertensive Disorders in Pregnancy

Ward Rounds in Obstetrics & Neonatology, 2016

Research paper thumbnail of Chapter-094 Fluid Management

Ward Rounds in Obstetrics & Neonatology, 2016

Research paper thumbnail of Efficacy of Magnesium Sulphate as an Adjunct to Ropivacaine in Local Infiltration for Postoperative Pain Following Lower Segment Caesarean Section

Journal of clinical and diagnostic research : JCDR, 2016

Intravenous and peri-articular magnesium has been shown to reduce perioperative analgesic consump... more Intravenous and peri-articular magnesium has been shown to reduce perioperative analgesic consumption. With this background, subcutaneous infiltration was hypothesized to potentiate the subcutaneous infiltration of local anaesthetic agent. To comparatively evaluate the efficacy of magnesium sulphate as an adjunct to ropivacaine in local infiltration for postoperative pain following lower segment cesarean section. Sixty parturients undergoing cesarean delivery were randomized to either group A or B in a double blinded manner. After uterine and muscle closure but before skin closure, Group A was administered local subcutaneous wound infiltration of Injection (Inj) ropivacaine 0.75% 150 milligram (mg) or 20 millilitres(ml) whereas, group B patients were given a local subcutaneous wound infiltration of Inj magnesium sulphate 750 mg (1.5 ml of Inj 50% Magnesium sulphate) added to Inj ropivacaine 0.75% (18.5 ml) making a total volume of 20 ml. In postoperative period, Heart rate (HR), Mea...

Research paper thumbnail of MafA-Controlled Nicotinic Receptor Expression Is Essential for Insulin Secretion and Is Impaired in Patients with Type 2 Diabetes

Cell Reports, 2016

Monoamine and acetylcholine neurotransmitters from the autonomic nervous system (ANS) regulate in... more Monoamine and acetylcholine neurotransmitters from the autonomic nervous system (ANS) regulate insulin secretion in pancreatic islets. The molecular mechanisms controlling neurotransmitter signaling in islet β cells and their impact on diabetes development are only partially understood. Using a glucose-intolerant, MafA-deficient mouse model, we demonstrate that MAFA controls ANS-mediated insulin secretion by activating the transcription of nicotinic (ChrnB2 and ChrnB4) and adrenergic (Adra2A) receptor genes, which are integral parts of acetylcholine- and monoamine-signaling pathways. We show that acetylcholine-mediated insulin secretion requires nicotinic signaling and that nicotinic receptor expression is positively correlated with insulin secretion and glycemic control in human donor islets. Moreover, polymorphisms spanning MAFA-binding regions within the human CHRNB4 gene are associated with type 2 diabetes. Our data show that MAFA transcriptional activity is required for establishing β cell sensitivity to neurotransmitter signaling and identify nicotinic signaling as a modulator of insulin secretion impaired in type 2 diabetes.

Research paper thumbnail of Abstract 17032: Novel risk score to predict in-hospital mortality in a contemporary Cardiac Intensive Care Unit

Circulation, Nov 25, 2014

Research paper thumbnail of Role of Non-invasive Imaging in the Work-Up of Cardiomyopathies

Current Atherosclerosis Reports, 2015

Cardiomyopathy is an intrinsic structural disorder of the myocardium that may result in ventricul... more Cardiomyopathy is an intrinsic structural disorder of the myocardium that may result in ventricular dysfunction and can progress to clinical heart failure. Non-invasive imaging modalities can determine whether abnormalities are present in the myocardium, valves, pericardium, or vessels. These imaging techniques can also present important prognostic information, as well as direct treatment and further management. Echocardiography is the most common imaging technique used for the initial diagnosis and management of cardiomyopathy; however, other imaging modalities, including nuclear cardiac imaging, cardiac magnetic resonance imaging, and cardiac computed tomography, may play an important role depending on the underlying etiology of the cardiomyopathy. This article reviews the role of non-invasive imaging in the work-up and management of patients with cardiomyopathy.

Research paper thumbnail of The triple line pattern on carotid intima media thickness imaging and its relationship to cardiovascular risk factors in patients on lipid lowering therapy

Vascular health and risk management, 2014

Carotid intima media thickness (CIMT) infrequently identifies a triple line pattern (TLP) in the ... more Carotid intima media thickness (CIMT) infrequently identifies a triple line pattern (TLP) in the visualization of the internal elastic lamina. We examined the prevalence and predictors of the TLP among a consecutive series of subjects enrolled in a CIMT clinical trial, and also the effects of lipid lowering therapy. Baseline CIMT studies of subjects with known heart disease, or high risk for heart disease, were evaluated from a single site of the Arterial Biology for the Investigation of the Treatment Effects of Reducing Cholesterol 6-HDL and LDL Treatment Strategies in Atherosclerosis trial (N=120). One sonographer obtained four views of the right and left far wall common CIMT, using a 13 MHz ultrasound probe. Images were blindly reviewed for the presence of the TLP. The TLP was defined as absent (0), possible (1), or definite (2). A composite score from all four views was calculated. A patient was defined as having the TLP if the composite score was ≥4. Univariate predictors of th...

Research paper thumbnail of Aortic Valve Calcium Score by Computed Tomography in Predicting Perivalvular Aortic Insufficiency Post Transcatheter Aortic Valve Implantation (Tavi)

Journal of the American College of Cardiology, 2012

Research paper thumbnail of Echocardiographic and Clinical Correlates of Specific Early Repolarization Subtypes on Electrocardiogram

Journal of the American College of Cardiology, 2012

Research paper thumbnail of Particle concentration measurement of virus samples using electrospray differential mobility analysis and quantitative amino acid analysis

Journal of Chromatography A, 2009

Virus reference materials are needed to develop and calibrate detection devices and instruments. ... more Virus reference materials are needed to develop and calibrate detection devices and instruments. We used electrospray differential mobility analysis (ES-DMA) and quantitative amino acid analysis (AAA) to determine the particle concentration of three small model viruses (bacteriophages MS2, PP7, and X174). The biological activity, purity, and aggregation of the virus samples were measured using plaque assays, denaturing gel electrophoresis, and size-exclusion chromatography. ES-DMA was developed to count the virus particles using gold nanoparticles as internal standards. ES-DMA additionally provides quantitative measurement of the size and extent of aggregation in the virus samples. Quantitative AAA was also used to determine the mass of the viral proteins in the pure virus samples. The samples were hydrolyzed and the masses of the well-recovered amino acids were used to calculate the equivalent concentration of viral particles in the samples. The concentration of the virus samples determined by ES-DMA was in good agreement with the concentration predicted by AAA for these purified samples. The advantages and limitations of ES-DMA and AAA to characterize virus reference materials are discussed.

Research paper thumbnail of Early Repolarization Pattern: Clinical Correlates of Specific Electrocardiographic Subtypes and Genome Wide Association Study of a Sardinian Population

Journal of the American College of Cardiology, Mar 27, 2012

Background: Early repolarization pattern (ERP) on electrocardiogram (ECG) is common in the genera... more Background: Early repolarization pattern (ERP) on electrocardiogram (ECG) is common in the general population. ERP, especially in the inferior distribution, has been associated with sudden cardiac death. The aim of this study was to assess clinical correlates and conduct a preliminary genome wide association study (GWAS) of ERP.

Research paper thumbnail of Comparison of two WHO partographs: a one year randomized controlled trial

Journal of the Turkish German Gynecological Association, 2011

To compare two World Health Organization (WHO) partographs - a composite partograph including the... more To compare two World Health Organization (WHO) partographs - a composite partograph including the latent phase with a simplified one without the latent phase in women with uncomplicated pregnancy. This was a randomized controlled trial conducted at a tertiary hospital at Belgaum, India. 743 women with term, singleton, vertex gestation, in spontaneous labor were included in the study over a period of one year. Either of the partographs was used on laboring women. The following outcomes were compared: labor crossing the alert and action line, augmentation of labor, rate of cesarean section, perinatal outcome, user friendliness and maternal complications. Statistical analysis was done using Chi-square test. Labor values crossed the alert and action lines significantly more often when composite partograph was used (p<0.001) in each, with increased number of augmentations (p<0.001). The number of vaginal deliveries were high (p<0.005) in the simplified group. There was no significant difference in the rate of cesarean deliveries due to non progress of labor in both groups (p=0.68). NICU admissions were higher in the composite group (p=0.035). Most resident doctors (93%) experienced difficulty with the composite partograph, but no resident doctor reported difficulty with the simplified partograph. The mean SD user friendliness score was lower for the composite partograph (2.87±1.86 vs 10.67±1.61; p<0.005). The WHO simplified partograph is easier to use and is a better option for both the laboring women and the user, when compared to composite partograph.

Research paper thumbnail of Echocardiographic and Clinical Correlates of Specific Early Repolarization Subtypes on Electrocardiogram

Journal of the American College of Cardiology, 2012

Research paper thumbnail of Chapter-092 Operative Site Infections

Ward Rounds in Obstetrics & Neonatology, 2016

Research paper thumbnail of Chapter-093 Blood Transfusion and Blood Components

Ward Rounds in Obstetrics & Neonatology, 2016

Research paper thumbnail of Chapter-017 Rh Negative Pregnancy

Ward Rounds in Obstetrics & Neonatology, 2016

Research paper thumbnail of Chapter-029 Hypertensive Disorders in Pregnancy

Ward Rounds in Obstetrics & Neonatology, 2016

Research paper thumbnail of Chapter-094 Fluid Management

Ward Rounds in Obstetrics & Neonatology, 2016

Research paper thumbnail of Efficacy of Magnesium Sulphate as an Adjunct to Ropivacaine in Local Infiltration for Postoperative Pain Following Lower Segment Caesarean Section

Journal of clinical and diagnostic research : JCDR, 2016

Intravenous and peri-articular magnesium has been shown to reduce perioperative analgesic consump... more Intravenous and peri-articular magnesium has been shown to reduce perioperative analgesic consumption. With this background, subcutaneous infiltration was hypothesized to potentiate the subcutaneous infiltration of local anaesthetic agent. To comparatively evaluate the efficacy of magnesium sulphate as an adjunct to ropivacaine in local infiltration for postoperative pain following lower segment cesarean section. Sixty parturients undergoing cesarean delivery were randomized to either group A or B in a double blinded manner. After uterine and muscle closure but before skin closure, Group A was administered local subcutaneous wound infiltration of Injection (Inj) ropivacaine 0.75% 150 milligram (mg) or 20 millilitres(ml) whereas, group B patients were given a local subcutaneous wound infiltration of Inj magnesium sulphate 750 mg (1.5 ml of Inj 50% Magnesium sulphate) added to Inj ropivacaine 0.75% (18.5 ml) making a total volume of 20 ml. In postoperative period, Heart rate (HR), Mea...

Research paper thumbnail of MafA-Controlled Nicotinic Receptor Expression Is Essential for Insulin Secretion and Is Impaired in Patients with Type 2 Diabetes

Cell Reports, 2016

Monoamine and acetylcholine neurotransmitters from the autonomic nervous system (ANS) regulate in... more Monoamine and acetylcholine neurotransmitters from the autonomic nervous system (ANS) regulate insulin secretion in pancreatic islets. The molecular mechanisms controlling neurotransmitter signaling in islet β cells and their impact on diabetes development are only partially understood. Using a glucose-intolerant, MafA-deficient mouse model, we demonstrate that MAFA controls ANS-mediated insulin secretion by activating the transcription of nicotinic (ChrnB2 and ChrnB4) and adrenergic (Adra2A) receptor genes, which are integral parts of acetylcholine- and monoamine-signaling pathways. We show that acetylcholine-mediated insulin secretion requires nicotinic signaling and that nicotinic receptor expression is positively correlated with insulin secretion and glycemic control in human donor islets. Moreover, polymorphisms spanning MAFA-binding regions within the human CHRNB4 gene are associated with type 2 diabetes. Our data show that MAFA transcriptional activity is required for establishing β cell sensitivity to neurotransmitter signaling and identify nicotinic signaling as a modulator of insulin secretion impaired in type 2 diabetes.

Research paper thumbnail of Abstract 17032: Novel risk score to predict in-hospital mortality in a contemporary Cardiac Intensive Care Unit

Circulation, Nov 25, 2014

Research paper thumbnail of Role of Non-invasive Imaging in the Work-Up of Cardiomyopathies

Current Atherosclerosis Reports, 2015

Cardiomyopathy is an intrinsic structural disorder of the myocardium that may result in ventricul... more Cardiomyopathy is an intrinsic structural disorder of the myocardium that may result in ventricular dysfunction and can progress to clinical heart failure. Non-invasive imaging modalities can determine whether abnormalities are present in the myocardium, valves, pericardium, or vessels. These imaging techniques can also present important prognostic information, as well as direct treatment and further management. Echocardiography is the most common imaging technique used for the initial diagnosis and management of cardiomyopathy; however, other imaging modalities, including nuclear cardiac imaging, cardiac magnetic resonance imaging, and cardiac computed tomography, may play an important role depending on the underlying etiology of the cardiomyopathy. This article reviews the role of non-invasive imaging in the work-up and management of patients with cardiomyopathy.

Research paper thumbnail of The triple line pattern on carotid intima media thickness imaging and its relationship to cardiovascular risk factors in patients on lipid lowering therapy

Vascular health and risk management, 2014

Carotid intima media thickness (CIMT) infrequently identifies a triple line pattern (TLP) in the ... more Carotid intima media thickness (CIMT) infrequently identifies a triple line pattern (TLP) in the visualization of the internal elastic lamina. We examined the prevalence and predictors of the TLP among a consecutive series of subjects enrolled in a CIMT clinical trial, and also the effects of lipid lowering therapy. Baseline CIMT studies of subjects with known heart disease, or high risk for heart disease, were evaluated from a single site of the Arterial Biology for the Investigation of the Treatment Effects of Reducing Cholesterol 6-HDL and LDL Treatment Strategies in Atherosclerosis trial (N=120). One sonographer obtained four views of the right and left far wall common CIMT, using a 13 MHz ultrasound probe. Images were blindly reviewed for the presence of the TLP. The TLP was defined as absent (0), possible (1), or definite (2). A composite score from all four views was calculated. A patient was defined as having the TLP if the composite score was ≥4. Univariate predictors of th...

Research paper thumbnail of Aortic Valve Calcium Score by Computed Tomography in Predicting Perivalvular Aortic Insufficiency Post Transcatheter Aortic Valve Implantation (Tavi)

Journal of the American College of Cardiology, 2012

Research paper thumbnail of Echocardiographic and Clinical Correlates of Specific Early Repolarization Subtypes on Electrocardiogram

Journal of the American College of Cardiology, 2012

Research paper thumbnail of Particle concentration measurement of virus samples using electrospray differential mobility analysis and quantitative amino acid analysis

Journal of Chromatography A, 2009

Virus reference materials are needed to develop and calibrate detection devices and instruments. ... more Virus reference materials are needed to develop and calibrate detection devices and instruments. We used electrospray differential mobility analysis (ES-DMA) and quantitative amino acid analysis (AAA) to determine the particle concentration of three small model viruses (bacteriophages MS2, PP7, and X174). The biological activity, purity, and aggregation of the virus samples were measured using plaque assays, denaturing gel electrophoresis, and size-exclusion chromatography. ES-DMA was developed to count the virus particles using gold nanoparticles as internal standards. ES-DMA additionally provides quantitative measurement of the size and extent of aggregation in the virus samples. Quantitative AAA was also used to determine the mass of the viral proteins in the pure virus samples. The samples were hydrolyzed and the masses of the well-recovered amino acids were used to calculate the equivalent concentration of viral particles in the samples. The concentration of the virus samples determined by ES-DMA was in good agreement with the concentration predicted by AAA for these purified samples. The advantages and limitations of ES-DMA and AAA to characterize virus reference materials are discussed.

Research paper thumbnail of Early Repolarization Pattern: Clinical Correlates of Specific Electrocardiographic Subtypes and Genome Wide Association Study of a Sardinian Population

Journal of the American College of Cardiology, Mar 27, 2012

Background: Early repolarization pattern (ERP) on electrocardiogram (ECG) is common in the genera... more Background: Early repolarization pattern (ERP) on electrocardiogram (ECG) is common in the general population. ERP, especially in the inferior distribution, has been associated with sudden cardiac death. The aim of this study was to assess clinical correlates and conduct a preliminary genome wide association study (GWAS) of ERP.