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Papers by Tania Valdes-Gonzalez

Research paper thumbnail of Preclinical evaluation of antioxidant, anti-inflammatory, cytotoxic and lipid-lowering properties of a nutraceutical from Curcuma longa cultivated in Cuba

Pharmacy & Pharmacology International Journal, 2019

In Cuba, Curcuma longa, is originally found in the mountainous regions of Pinar del Rio and Santi... more In Cuba, Curcuma longa, is originally found in the mountainous regions of Pinar del Rio and Santiago de Cuba. Since ancient times this plant has been used primarily in Asia as a food, cosmetic and herbal medicine, nevertheless in our country there is not report of the traditional use. Due to population aging and the increase of chronic non communicable diseases, the development of nutraceuticals has become of interest in our country. In the present work we evaluated a nutraceutical prepared with Curcuma longa cultivated in the Artemisa region. We determined the antioxidant activity by DPPH and FRAP methods, the anti-inflammatory activity in vivo in the cotton disc-induced granuloma model, the cytotoxic effect in the human tumor line NCI-H460 and the lipid-lowering properties in knockout ApoE mice. The results showed that the nutraceutical is capable of sequestering DPPH depending on the concentration used, with a mean inhibitory concentration of 150ug/ml and high ferroreductive activity even at the concentration of 0.25mg/ml. In the granuloma model in mice the nutraceutical reduced systemic inflammation and cell infiltration and inhibited the formation of granuloma in a dose-dependent effect with an average effective dose of 3,245mg/kg, which is interesting in a natural product. It also showed cytotoxic potential in the human tumor cells NCI-H460 with a high anti-proliferative activity in this line. The trials with APOE knockout mice indicated that the nutraceutical not only promotes lipid lowering and atheroma decreasing, but also improves vessel wall condition in atherosclerotic pathology.

Research paper thumbnail of Membrane Ganglioside Composition: Important Factor in Beta-Amyloid Accumulation on cell Surface

Research paper thumbnail of Important factor for the deposition of beta-amyloid peptides in the brain : The composition of major gangliosides in neuronal cell membrane

Research paper thumbnail of Characterization of Neuropeptide Interactions with Glycolipid Receptors by Surface Plasmon Resonance

Research paper thumbnail of Extrait contenant de l'équol, son procédé de production, procédé d'extraction de l'équol, et aliment contenant de l'équol

La presente invention concerne un extrait produit en extrayant un composant de choix contenant de... more La presente invention concerne un extrait produit en extrayant un composant de choix contenant de l'equol d'un produit de fermentation contenant de l'equol issu d'un axe embryonnaire de soja. La presente invention concerne egalement un procede de production de l'extrait. Un produit de fermentation contenant de l'equol issu d'un axe embryonnaire de soja est extrait en utilisant une solution d'ethanol aqueux comme solvant d'extraction pour extraire un composant de choix contenant de l'equol du produit de fermentation avec un degre d'efficacite eleve. En variante, un produit de fermentation contenant de l'equol issu d'un axe embryonnaire de soja est soumis successivement a l'extraction avec une solution d'ethanol aqueux et l'extrait avec l'ethanol pour extraire l'equol et la glyciteine avec un haut degre d'efficacite, reduisant de ce fait la teneur en saponine qui donne un gout desagreable.

Research paper thumbnail of An extract containing equol (EQUOL), a method for producing the same, an equol extraction method and an equol-containing food

Research paper thumbnail of 柑橘類成分F-1の記憶・学習障害改善作用とそのメカニズム

Research paper thumbnail of Analysis of potential anti-aging beverage Pru, a traditional Cuban refreshment, by desorption electrospray ionization-mass spectrometry and FTICR tandem mass spectrometry

Journal of Food and Drug Analysis

Research paper thumbnail of A new lipidomics approach by thin-layer chromatography-blot-matrix-assisted laser desorption/ionization imaging mass spectrometry for analyzing detailed patterns of phospholipid molecular species

Thin-layer chromatography (TLC) is a highly established convenient technique for lipid separation... more Thin-layer chromatography (TLC) is a highly established convenient technique for lipid separation and partial characterization of neutral and acidic glycosphingolipids (GSLs) and phospholipids, in mixtures. Meanwhile, imaging mass spectrometry (IMS) is a promising tool for lipidomics. However, some lipid classes are detected more sensitively than others, which can lead to suppression effects when complex mixtures are analyzed. Therefore to analyze complex lipid mixtures, a precise separation into the individual lipid classes is necessary. Here we present our highly sensitive and convenient analytical technology that combines TLC and IMS, namely the TLC-Blot-MALDI-IMS method, to visualize whole lipids and individual molecular species with high sensitivity compared with common staining methods. This method allows for easy visualization of all lipids with a linear range of approximately one order of magnitude and precision <16% RSD, making it useful for differential display analysis of lipids.

Research paper thumbnail of Imaging Technology of Complex Lipid Molecular Species by a Combination of TLC-Blot and MALDI-TOF –Special Reference to Human Brain Ganglioside Molecular Species

Journal of Glycomics & Lipidomics, 2012

Glycosphingolipids and phospholipids from white matter of human hippocampus, were analyzed by a s... more Glycosphingolipids and phospholipids from white matter of human hippocampus, were analyzed by a sequential procedure of two-dimensional TLC (2d-TLC), transference of separated lipids to a PVDF membrane by a TLC-Blot equipment and mass spectrometry (MS) analysis with an ion-trap type MALDI-TOF equipment. The method is simple and quick; very small amount of sample (0.1 mg of brain tissue) is enough to analyze all lipid components. The 2d-TLC provided excellent separation and the MS analyses allowed identifying the characteristic profile of molecular species for individual glycolipids and phospholipids. The results of MS analyses on gangliosides showed that di-and tri-sialogangliosides are richer in d20:1 sphingosine-containing ceramide than monosialogangliosides, suggesting the presence of sialylation selection after GM1 gangliosides. Then we analyzed ganglioside molecular species obtained from different brain regions by using MS imaging technology. The MS images of individual gangliosides provided clear visual profiles in terms of molecular species distribution. The imaging profiles were region dependent and also indicated that the sialyltransferase toward GM1 ganglioside prefers to select d20:1 sphingosine containing molecule. This technology provides visual characterization of individual phospholipid and glycosphingolipid molecular species and informs us about the metabolic characterization of target tissue, opening a new gate for colorful lipidomics research.

Research paper thumbnail of Characterization of the interactions of β‒amyloid peptides with glycolipid receptors by surface plasmon resonance

Spectroscopy, 2003

Interactions betweenβ‒amyloid (Aβ) peptides and neuronal membranes play an important role in Alzh... more Interactions betweenβ‒amyloid (Aβ) peptides and neuronal membranes play an important role in Alzheimer's disease (AD). Using surface plasmon resonance we assayed a kinetic model to study the interactions of Aβ25‒35, Aβ40 and Aβ42 with surfaces containing single glycolipids (Asialo‒GM1, GM1, GD1a or GT1b). The larger peptides interacted with gangliosides stronger than Aβ25‒35, which showed some significant bindings solely at high concentrations under acidic conditions. Only the interactions at low Aβconcentrations were useful to calculate the kinetic constants. The affinities increased at low pH. The specificity, but not the affinity correlated with the number of sialic acids in the ganglioside sugar moiety. The most important finding in this study, was a special group of sensorgrams with linear association phases that appeared for the interactions of Aβwith the membranes containing gangliosides, due to the following process: when Aβis injected at a critical concentration, the fi...

Research paper thumbnail of P4-389 Neuropeptides interfere and dissolve the beta-amyloid accumulation on cell surface

Neurobiology of Aging, 2004

Research paper thumbnail of Does Small-Dose .GAMMA.-Ray Radiation Induce Endogenous Antioxidant Potential in Vivo?

Biological & Pharmaceutical Bulletin, 1997

Research paper thumbnail of New approach for glyco‐and lipidomics–Molecular scanning of human brain gangliosides by TLC‐Blot and MALDI‐QIT‐TOF MS

The gangliosides, which are sialic acid-containing glycosphingolipids, are major constituents of ... more The gangliosides, which are sialic acid-containing glycosphingolipids, are major constituents of neuronal cell membranes. Like other glycosphingolipids, gangliosides are located in the outer leaflet of the cell membrane and identified as constituents of the raft microdomain, where they play important roles in the early events of signal transduction by arranging the environment of ligandreceptor interactions (Prinetti et al. 1999; Janich and Corbeil 2007). In these events, not only the carbohydrate moieties but also ceramide moieties are important to facilitate the formation of a microdomain environment. To elucidate the functional roles of gangliosides, studies involving exogenous addition of individual gangliosides to a culture system (Hanai et al. 1988; Weis and Davis 1990; Ledeen and Wu 2002), production of glycosphingolipid synthase-knockout mice (Zitman et al. 2008; Ohmi et al. 2009; Tajima et al. 2010), and investigation of the effects of anti-ganglioside antibodies and ganglioside mimetic peptides have been performed (

Research paper thumbnail of Excitotoxicity induces changes in rat brain gangliosides

Neuroscience Research, 2001

The patterns of major gangliosides in the rat hippocampi and olfactory bulbs was examined in vivo... more The patterns of major gangliosides in the rat hippocampi and olfactory bulbs was examined in vivo after microinjections of Ibotenic acid and L-BOAA (NMDA and AMPA receptor agonists, respectively) which were given under free-movement conditions. The excitotoxicity induced by injections of Ibotenic acid promoted transient ganglioside changes in olfactory bulbs and permanent changes in hippocampus. Four days after injections, the amount of gangliosides in the hippocampus increased significantly for GQ1b, GT1b and GD1b and decreased in the olfactory bulb for GQ1b, GT1b, GD1b, GD1a and GM1 compared to normal ganglioside levels. The alterations of gangliosides were minimal 1 day after injections. After 5 weeks, the amounts of GQ1b, GT1b and GD1b dramatically decreased in the hippocampus while in the olfactory bulbs gangliosides recovered to normal levels. The results obtained with L-BOAA 4 days after injections strengthen the results observed in the experiments using Ibotenic acid and corroborate our suggestion that gangliosides have an active role in the compensatory mechanism to maintain the number of glutamate receptors during the excitotoxicity.

Research paper thumbnail of Neuropeptides interact with glycolipid receptors: a surface plasmon resonance study

Peptides, 2001

Using Surface Plasmon Resonance (SPR) we investigated the interaction of seven neuropeptides with... more Using Surface Plasmon Resonance (SPR) we investigated the interaction of seven neuropeptides with different characteristics and beta-amyloid (Abeta42) peptide, with membranes containing gangliosides. A wide range of affinities characterized the bindings (K(D) = 10(-3)- 10(-7) M), following the scheme: for GM1, Abeta42 > DYN > SP = GAL = SOM = BRD > OXY = ENK; for GD1a, Abeta42 = DYN = GAL > SP = SOM = BRD = OXY > ENK and for GT1b, Abeta42 > DYN > SP = GAL > SOM = BRD = OXY > ENK. The ganglioside sugar moiety, specifically the sialic acid, had an important role in the interactions. In general the affinities were higher with polysialo, than with monosialo gangliosides. The sensorgrams describing the interactions of Abeta42 and SP with gangliosides differed from the interactions of the other studied peptides. Ca(2+) promoted changes in peptide-glycolipid interactions.

Research paper thumbnail of Preclinical evaluation of antioxidant, anti-inflammatory, cytotoxic and lipid-lowering properties of a nutraceutical from Curcuma longa cultivated in Cuba

Pharmacy & Pharmacology International Journal, 2019

In Cuba, Curcuma longa, is originally found in the mountainous regions of Pinar del Rio and Santi... more In Cuba, Curcuma longa, is originally found in the mountainous regions of Pinar del Rio and Santiago de Cuba. Since ancient times this plant has been used primarily in Asia as a food, cosmetic and herbal medicine, nevertheless in our country there is not report of the traditional use. Due to population aging and the increase of chronic non communicable diseases, the development of nutraceuticals has become of interest in our country. In the present work we evaluated a nutraceutical prepared with Curcuma longa cultivated in the Artemisa region. We determined the antioxidant activity by DPPH and FRAP methods, the anti-inflammatory activity in vivo in the cotton disc-induced granuloma model, the cytotoxic effect in the human tumor line NCI-H460 and the lipid-lowering properties in knockout ApoE mice. The results showed that the nutraceutical is capable of sequestering DPPH depending on the concentration used, with a mean inhibitory concentration of 150ug/ml and high ferroreductive activity even at the concentration of 0.25mg/ml. In the granuloma model in mice the nutraceutical reduced systemic inflammation and cell infiltration and inhibited the formation of granuloma in a dose-dependent effect with an average effective dose of 3,245mg/kg, which is interesting in a natural product. It also showed cytotoxic potential in the human tumor cells NCI-H460 with a high anti-proliferative activity in this line. The trials with APOE knockout mice indicated that the nutraceutical not only promotes lipid lowering and atheroma decreasing, but also improves vessel wall condition in atherosclerotic pathology.

Research paper thumbnail of Membrane Ganglioside Composition: Important Factor in Beta-Amyloid Accumulation on cell Surface

Research paper thumbnail of Important factor for the deposition of beta-amyloid peptides in the brain : The composition of major gangliosides in neuronal cell membrane

Research paper thumbnail of Characterization of Neuropeptide Interactions with Glycolipid Receptors by Surface Plasmon Resonance

Research paper thumbnail of Extrait contenant de l'équol, son procédé de production, procédé d'extraction de l'équol, et aliment contenant de l'équol

La presente invention concerne un extrait produit en extrayant un composant de choix contenant de... more La presente invention concerne un extrait produit en extrayant un composant de choix contenant de l'equol d'un produit de fermentation contenant de l'equol issu d'un axe embryonnaire de soja. La presente invention concerne egalement un procede de production de l'extrait. Un produit de fermentation contenant de l'equol issu d'un axe embryonnaire de soja est extrait en utilisant une solution d'ethanol aqueux comme solvant d'extraction pour extraire un composant de choix contenant de l'equol du produit de fermentation avec un degre d'efficacite eleve. En variante, un produit de fermentation contenant de l'equol issu d'un axe embryonnaire de soja est soumis successivement a l'extraction avec une solution d'ethanol aqueux et l'extrait avec l'ethanol pour extraire l'equol et la glyciteine avec un haut degre d'efficacite, reduisant de ce fait la teneur en saponine qui donne un gout desagreable.

Research paper thumbnail of An extract containing equol (EQUOL), a method for producing the same, an equol extraction method and an equol-containing food

Research paper thumbnail of 柑橘類成分F-1の記憶・学習障害改善作用とそのメカニズム

Research paper thumbnail of Analysis of potential anti-aging beverage Pru, a traditional Cuban refreshment, by desorption electrospray ionization-mass spectrometry and FTICR tandem mass spectrometry

Journal of Food and Drug Analysis

Research paper thumbnail of A new lipidomics approach by thin-layer chromatography-blot-matrix-assisted laser desorption/ionization imaging mass spectrometry for analyzing detailed patterns of phospholipid molecular species

Thin-layer chromatography (TLC) is a highly established convenient technique for lipid separation... more Thin-layer chromatography (TLC) is a highly established convenient technique for lipid separation and partial characterization of neutral and acidic glycosphingolipids (GSLs) and phospholipids, in mixtures. Meanwhile, imaging mass spectrometry (IMS) is a promising tool for lipidomics. However, some lipid classes are detected more sensitively than others, which can lead to suppression effects when complex mixtures are analyzed. Therefore to analyze complex lipid mixtures, a precise separation into the individual lipid classes is necessary. Here we present our highly sensitive and convenient analytical technology that combines TLC and IMS, namely the TLC-Blot-MALDI-IMS method, to visualize whole lipids and individual molecular species with high sensitivity compared with common staining methods. This method allows for easy visualization of all lipids with a linear range of approximately one order of magnitude and precision <16% RSD, making it useful for differential display analysis of lipids.

Research paper thumbnail of Imaging Technology of Complex Lipid Molecular Species by a Combination of TLC-Blot and MALDI-TOF –Special Reference to Human Brain Ganglioside Molecular Species

Journal of Glycomics & Lipidomics, 2012

Glycosphingolipids and phospholipids from white matter of human hippocampus, were analyzed by a s... more Glycosphingolipids and phospholipids from white matter of human hippocampus, were analyzed by a sequential procedure of two-dimensional TLC (2d-TLC), transference of separated lipids to a PVDF membrane by a TLC-Blot equipment and mass spectrometry (MS) analysis with an ion-trap type MALDI-TOF equipment. The method is simple and quick; very small amount of sample (0.1 mg of brain tissue) is enough to analyze all lipid components. The 2d-TLC provided excellent separation and the MS analyses allowed identifying the characteristic profile of molecular species for individual glycolipids and phospholipids. The results of MS analyses on gangliosides showed that di-and tri-sialogangliosides are richer in d20:1 sphingosine-containing ceramide than monosialogangliosides, suggesting the presence of sialylation selection after GM1 gangliosides. Then we analyzed ganglioside molecular species obtained from different brain regions by using MS imaging technology. The MS images of individual gangliosides provided clear visual profiles in terms of molecular species distribution. The imaging profiles were region dependent and also indicated that the sialyltransferase toward GM1 ganglioside prefers to select d20:1 sphingosine containing molecule. This technology provides visual characterization of individual phospholipid and glycosphingolipid molecular species and informs us about the metabolic characterization of target tissue, opening a new gate for colorful lipidomics research.

Research paper thumbnail of Characterization of the interactions of β‒amyloid peptides with glycolipid receptors by surface plasmon resonance

Spectroscopy, 2003

Interactions betweenβ‒amyloid (Aβ) peptides and neuronal membranes play an important role in Alzh... more Interactions betweenβ‒amyloid (Aβ) peptides and neuronal membranes play an important role in Alzheimer's disease (AD). Using surface plasmon resonance we assayed a kinetic model to study the interactions of Aβ25‒35, Aβ40 and Aβ42 with surfaces containing single glycolipids (Asialo‒GM1, GM1, GD1a or GT1b). The larger peptides interacted with gangliosides stronger than Aβ25‒35, which showed some significant bindings solely at high concentrations under acidic conditions. Only the interactions at low Aβconcentrations were useful to calculate the kinetic constants. The affinities increased at low pH. The specificity, but not the affinity correlated with the number of sialic acids in the ganglioside sugar moiety. The most important finding in this study, was a special group of sensorgrams with linear association phases that appeared for the interactions of Aβwith the membranes containing gangliosides, due to the following process: when Aβis injected at a critical concentration, the fi...

Research paper thumbnail of P4-389 Neuropeptides interfere and dissolve the beta-amyloid accumulation on cell surface

Neurobiology of Aging, 2004

Research paper thumbnail of Does Small-Dose .GAMMA.-Ray Radiation Induce Endogenous Antioxidant Potential in Vivo?

Biological & Pharmaceutical Bulletin, 1997

Research paper thumbnail of New approach for glyco‐and lipidomics–Molecular scanning of human brain gangliosides by TLC‐Blot and MALDI‐QIT‐TOF MS

The gangliosides, which are sialic acid-containing glycosphingolipids, are major constituents of ... more The gangliosides, which are sialic acid-containing glycosphingolipids, are major constituents of neuronal cell membranes. Like other glycosphingolipids, gangliosides are located in the outer leaflet of the cell membrane and identified as constituents of the raft microdomain, where they play important roles in the early events of signal transduction by arranging the environment of ligandreceptor interactions (Prinetti et al. 1999; Janich and Corbeil 2007). In these events, not only the carbohydrate moieties but also ceramide moieties are important to facilitate the formation of a microdomain environment. To elucidate the functional roles of gangliosides, studies involving exogenous addition of individual gangliosides to a culture system (Hanai et al. 1988; Weis and Davis 1990; Ledeen and Wu 2002), production of glycosphingolipid synthase-knockout mice (Zitman et al. 2008; Ohmi et al. 2009; Tajima et al. 2010), and investigation of the effects of anti-ganglioside antibodies and ganglioside mimetic peptides have been performed (

Research paper thumbnail of Excitotoxicity induces changes in rat brain gangliosides

Neuroscience Research, 2001

The patterns of major gangliosides in the rat hippocampi and olfactory bulbs was examined in vivo... more The patterns of major gangliosides in the rat hippocampi and olfactory bulbs was examined in vivo after microinjections of Ibotenic acid and L-BOAA (NMDA and AMPA receptor agonists, respectively) which were given under free-movement conditions. The excitotoxicity induced by injections of Ibotenic acid promoted transient ganglioside changes in olfactory bulbs and permanent changes in hippocampus. Four days after injections, the amount of gangliosides in the hippocampus increased significantly for GQ1b, GT1b and GD1b and decreased in the olfactory bulb for GQ1b, GT1b, GD1b, GD1a and GM1 compared to normal ganglioside levels. The alterations of gangliosides were minimal 1 day after injections. After 5 weeks, the amounts of GQ1b, GT1b and GD1b dramatically decreased in the hippocampus while in the olfactory bulbs gangliosides recovered to normal levels. The results obtained with L-BOAA 4 days after injections strengthen the results observed in the experiments using Ibotenic acid and corroborate our suggestion that gangliosides have an active role in the compensatory mechanism to maintain the number of glutamate receptors during the excitotoxicity.

Research paper thumbnail of Neuropeptides interact with glycolipid receptors: a surface plasmon resonance study

Peptides, 2001

Using Surface Plasmon Resonance (SPR) we investigated the interaction of seven neuropeptides with... more Using Surface Plasmon Resonance (SPR) we investigated the interaction of seven neuropeptides with different characteristics and beta-amyloid (Abeta42) peptide, with membranes containing gangliosides. A wide range of affinities characterized the bindings (K(D) = 10(-3)- 10(-7) M), following the scheme: for GM1, Abeta42 > DYN > SP = GAL = SOM = BRD > OXY = ENK; for GD1a, Abeta42 = DYN = GAL > SP = SOM = BRD = OXY > ENK and for GT1b, Abeta42 > DYN > SP = GAL > SOM = BRD = OXY > ENK. The ganglioside sugar moiety, specifically the sialic acid, had an important role in the interactions. In general the affinities were higher with polysialo, than with monosialo gangliosides. The sensorgrams describing the interactions of Abeta42 and SP with gangliosides differed from the interactions of the other studied peptides. Ca(2+) promoted changes in peptide-glycolipid interactions.