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Papers by Tararat Khaokhiew
Journal of Health Science, 2019
Dementia is a major threat for the aging. This cross-sectional study was to analyze physical act... more Dementia is a major threat for the aging. This cross-sectional study was to analyze physical activity and reducing the risk factors for dementia among aging female. Key informants included 192 aging women aged between 60-69 years divided into two groups: the first group - 144 persons, or the exercise group, was further divided into sub-groups of 8 persons who were assigned to practice 6 exercises, 3 sub-groups per one type, altogether 18 sub-groups for 6 types of exercise. The second group (48 persons) was the non-exercise group which was further divided into 6 sub-groups of 8 persons, and the members of each subgroup stayed in the vicinity of each type of the exercise groups. The study tools were interview form, record form and Thai Geriatric Depression Scale (TGDS) for the collection of relevant data. Frequency, percentage, chi-square and odds ratio were use in the data analysis. The results revealed that the group with physical inactivity had 4.75 times higher risk for dementia...
Sodium chloride, commonly known as table salt, is widely used as essential seasoning in food, sna... more Sodium chloride, commonly known as table salt, is widely used as essential seasoning in food, snacks and deserts worldwide. However, excessive consumption of table salt is a major cause of various health issues, involving high blood pressure, liver cirrhosis, kidney disease, and heart failure. This research aims at creating a portable, low-powered, efficient sensor for detection of sodium ions in body plasma for medical diagnosis purpose. The device was fabricated on a platform of Ion-Sensitive Field-Effect Transistor (ISFET) modified with sodium ionophore (sodium recognizing element), entrapped in polyurethane thin film. Our preliminary studies show that sodium ionophore-modified ISFET sensor yields good sensing performances, having a maximum sensitivity of 43 mV/pNa, and a detection limit of 2.3 millimol/liter.
Biochemical and Biophysical Research Communications, 2013
FEMS microbiology …, 2009
Surface-exposed loop residues, Pro 389 (b 6-b 7 loop), Glu 417 (b 8-b 9 loop), Tyr 455 and Asn 45... more Surface-exposed loop residues, Pro 389 (b 6-b 7 loop), Glu 417 (b 8-b 9 loop), Tyr 455 and Asn 456 (b 10-b 11 loop), in the receptor-binding domain of the Bacillus thuringiensis Cry4Ba toxin have been previously demonstrated to be crucial for toxicity. Herein, five combinations of two-loop mutants, P389A/E417A (b 6-b 7 / b 8-b 9 loops), P389A/Y455A, P389A/N456A (b 6-b 7 /b 10-b 11 loops), E417A/Y455A and E417A/N456A (b 8-b 9 /b 10-b 11 loops), were constructed as a means of examining a correlative effect of these three critical loops on Cry4Ba toxicity. All 130-kDa mutant protoxins were overexpressed as inclusion bodies in Escherichia coli with yields comparable to the wild-type toxin. In addition, all mutant toxins were structurally stable upon solubilization and trypsin activation in carbonate buffer, pH 9.0. Interestingly, E. coli cells expressing all the double-loop mutants showed an almost complete loss in toxicity against Aedes aegypti mosquito larvae while their corresponding single-loop mutants exhibited reduced activity of approximately 50%. Moreover, in situ binding analysis revealed that the 65-kDa purified toxins representing each pairwise mutant exhibited reduced binding to apical microvilli of A. aegypti larval midgut when compared with the single mutants. Altogether, the data demonstrate for the first time that all these three surface-exposed loops of the Cry4Ba toxin are equally involved in receptor binding and hence toxicity.
Oxidized Low Density Lipoprotein (OxLDL) is a crucial contributor of atherosclerosis by inducing ... more Oxidized Low Density Lipoprotein (OxLDL) is a crucial contributor of atherosclerosis by inducing tissue damage and inflammation of the arterial wall. The present of atherosclerosis inside the blood vessel wall directly affects to blood flow rate which closely relates to the risk of heart attack and stroke. The elevation of OxLDL in plasma is also associated with pathogenesis of atherosclerosis. Therefore, OxLDL has been recognized as the biomarker for atherosclerosis events. The reliable detection method for this biomarker is recently essential for atherosclerosis risk assessment. The piezoelectric immunosensor with modified quartz crystal surface was developed as a detection system to determine OxLDL concentration upon the binding interaction between OxLDL and anti-OxLDL. The anti-OxLDL was immobilized on silver electrode surface of 10 MHz AT-cut piezoelectric quartz crystal via self-assembled monolayer (SAM) of mercaptopropionic acid (MPA). The optimal anti-OxLDL immobilization wa...
Journal of Health Science, 2019
Dementia is a major threat for the aging. This cross-sectional study was to analyze physical act... more Dementia is a major threat for the aging. This cross-sectional study was to analyze physical activity and reducing the risk factors for dementia among aging female. Key informants included 192 aging women aged between 60-69 years divided into two groups: the first group - 144 persons, or the exercise group, was further divided into sub-groups of 8 persons who were assigned to practice 6 exercises, 3 sub-groups per one type, altogether 18 sub-groups for 6 types of exercise. The second group (48 persons) was the non-exercise group which was further divided into 6 sub-groups of 8 persons, and the members of each subgroup stayed in the vicinity of each type of the exercise groups. The study tools were interview form, record form and Thai Geriatric Depression Scale (TGDS) for the collection of relevant data. Frequency, percentage, chi-square and odds ratio were use in the data analysis. The results revealed that the group with physical inactivity had 4.75 times higher risk for dementia...
Sodium chloride, commonly known as table salt, is widely used as essential seasoning in food, sna... more Sodium chloride, commonly known as table salt, is widely used as essential seasoning in food, snacks and deserts worldwide. However, excessive consumption of table salt is a major cause of various health issues, involving high blood pressure, liver cirrhosis, kidney disease, and heart failure. This research aims at creating a portable, low-powered, efficient sensor for detection of sodium ions in body plasma for medical diagnosis purpose. The device was fabricated on a platform of Ion-Sensitive Field-Effect Transistor (ISFET) modified with sodium ionophore (sodium recognizing element), entrapped in polyurethane thin film. Our preliminary studies show that sodium ionophore-modified ISFET sensor yields good sensing performances, having a maximum sensitivity of 43 mV/pNa, and a detection limit of 2.3 millimol/liter.
Biochemical and Biophysical Research Communications, 2013
FEMS microbiology …, 2009
Surface-exposed loop residues, Pro 389 (b 6-b 7 loop), Glu 417 (b 8-b 9 loop), Tyr 455 and Asn 45... more Surface-exposed loop residues, Pro 389 (b 6-b 7 loop), Glu 417 (b 8-b 9 loop), Tyr 455 and Asn 456 (b 10-b 11 loop), in the receptor-binding domain of the Bacillus thuringiensis Cry4Ba toxin have been previously demonstrated to be crucial for toxicity. Herein, five combinations of two-loop mutants, P389A/E417A (b 6-b 7 / b 8-b 9 loops), P389A/Y455A, P389A/N456A (b 6-b 7 /b 10-b 11 loops), E417A/Y455A and E417A/N456A (b 8-b 9 /b 10-b 11 loops), were constructed as a means of examining a correlative effect of these three critical loops on Cry4Ba toxicity. All 130-kDa mutant protoxins were overexpressed as inclusion bodies in Escherichia coli with yields comparable to the wild-type toxin. In addition, all mutant toxins were structurally stable upon solubilization and trypsin activation in carbonate buffer, pH 9.0. Interestingly, E. coli cells expressing all the double-loop mutants showed an almost complete loss in toxicity against Aedes aegypti mosquito larvae while their corresponding single-loop mutants exhibited reduced activity of approximately 50%. Moreover, in situ binding analysis revealed that the 65-kDa purified toxins representing each pairwise mutant exhibited reduced binding to apical microvilli of A. aegypti larval midgut when compared with the single mutants. Altogether, the data demonstrate for the first time that all these three surface-exposed loops of the Cry4Ba toxin are equally involved in receptor binding and hence toxicity.
Oxidized Low Density Lipoprotein (OxLDL) is a crucial contributor of atherosclerosis by inducing ... more Oxidized Low Density Lipoprotein (OxLDL) is a crucial contributor of atherosclerosis by inducing tissue damage and inflammation of the arterial wall. The present of atherosclerosis inside the blood vessel wall directly affects to blood flow rate which closely relates to the risk of heart attack and stroke. The elevation of OxLDL in plasma is also associated with pathogenesis of atherosclerosis. Therefore, OxLDL has been recognized as the biomarker for atherosclerosis events. The reliable detection method for this biomarker is recently essential for atherosclerosis risk assessment. The piezoelectric immunosensor with modified quartz crystal surface was developed as a detection system to determine OxLDL concentration upon the binding interaction between OxLDL and anti-OxLDL. The anti-OxLDL was immobilized on silver electrode surface of 10 MHz AT-cut piezoelectric quartz crystal via self-assembled monolayer (SAM) of mercaptopropionic acid (MPA). The optimal anti-OxLDL immobilization wa...