Tarek Saleh - Academia.edu (original) (raw)
Papers by Tarek Saleh
Jacquard weaving provides the opportunity to design complex pictorial and other patterning effect... more Jacquard weaving provides the opportunity to design complex pictorial and other patterning effects From the combination of warp and weft colors and /weaves. In the traditional fabric design process, the resultant visual perception of the design, using different colored yarns, can be attained only through the production of actual physical fabric sample, and this is every time consuming process. No truly accurate digital color methodology is yet available to assist designers in the initial development of product samples. Currently, there is very poor correlation between the color that is shown on the screen and the actual weave structure. On The following research we back to the nature as a main source of colors by using digital photos of several nature sources such as water, grass, clouds, sunrise, and sunset…..ECT. And by analyzing colors for this natural sources by the computer processes to implement color data base to be reference for the designer helping him to select warp and weft colors also the mix between warp and weft from several color verities and use the correct weaves to rich this color. Experimental work: The researchers select some patterns from nature sources to be an example for the application directed to jacquard upholstery fabrics. Colors selection:-Select warp colors. To select warp color from the data base established by the computer processses designer should select (X) parameters based on the weaving technique used for producing the upholstery fabric the number of warp colors should match with the fabric specification and according to the weave construction. Also the designer should specify the percentage of warp color appearance on the fabric buy presenting suggested specification to implement the correct fabric simulation in the warp direction.
The present experiment was performed to evaluate the autologous grafting of superficial digital f... more The present experiment was performed to evaluate the autologous grafting of superficial digital flexor tendon with cunean tendon in donkeys. It was carried out on 24 adult donkeys equally divided into 2 groups. Full thickness tenectomy with suturing leaving a gap of 2 cm of the mid- metacarpal segment of the SDFT was performed in the control group. Full thickness tenectomy followed by grafting with cunean tendon was performed in the second group. Both groups were evaluated at 6, 12 and 24 weeks P.O. No significant differences (P>0.05) was observed between the mean values of mid-metacarpal limb circumferences of both groups at all times of examination. The mean values of the dorsal fetlock angle showed non-significant differences (P>0.05) between both groups at all times of examination. At 12 weeks P.O., lameness was observed only in the control group. The sagittal gray-scale ultrasonic examinations showed that the grafted tendons had higher echogenicity than the control tendo...
Cells, 2021
The functionalization of decellularized scaffolds is still challenging because of the recellulari... more The functionalization of decellularized scaffolds is still challenging because of the recellularization-related limitations, including the finding of the most optimal kind of cell(s) and the best way to control their distribution within the scaffolds to generate native mimicking tissues. That is why researchers have been encouraged to study stem cells, in particular, mesenchymal stem cells (MSCs), as alternative cells to repopulate and functionalize the scaffolds properly. MSCs could be obtained from various sources and have therapeutic effects on a wide range of inflammatory/degenerative diseases. Therefore, in this mini-review, we will discuss the benefits using of MSCs for recellularization, the factors affecting their efficiency, and the drawbacks that may need to be overcome to generate bioengineered transplantable organs.
Journal of Biomedical Materials Research Part A, 2020
Acute liver failure (ALF) occurs due to severe liver damage that triggers rapid loss of normal li... more Acute liver failure (ALF) occurs due to severe liver damage that triggers rapid loss of normal liver function. Here, we investigate the usefulness of an injectable liver extracellular matrix (LECM)-rich hydrogel generated from an optimized decellularization protocol incorporated with silver nanoparticles (AgNPs) as a promising therapy for ALF. First, we optimized a non-destructive protocol for rat liver decellularization to obtain ECM-rich well-preserved scaffold. Then, LECM hydrogel generated from two commonly used decellularization protocols were compared by LECM hydrogel obtained from our optimized protocol. The ALF model was induced by an intraperitoneal (IP) thioacetamide (TAA) injection followed by the IP injection of LECM hydrogel, collagen-AgNPs mixture or LECM hydrogel-AgNPs mixture. LECM-rich scaffold and hydrogel were successfully obtained using our optimized decellularization protocol. Use of the LECM hydrogel-AgNPs mixture to treat TAA induced ALF, greatly improving liver injury and histological liver regeneration. Interleukin-6 (IL-6) and transforming growth factor beta ( TGF-β) expressions were significantly reduced, while albumin, hepatocyte growth factor, and Ki67-positive cells were highly expressed. Moreover, aspartate transaminase (AST) and alanine transaminase (ALT) plasma levels, and liver homogenate nitric oxide (NO) level were significantly lowered. In conclusion, the LECM hydrogel-AgNPs mixture has potential efficient therapeutic and regenerative effects on TAA-induced liver injury. This article is protected by copyright. All rights reserved.
Journal of Bioscience and Bioengineering, 2019
Comparative Clinical Pathology, 2019
Tendon injury is one of the most common causes of wastage in equine. The superficial digital flex... more Tendon injury is one of the most common causes of wastage in equine. The superficial digital flexor tendon (SDFT) gets affected the most, especially in the mid-metacarpal region. Several studies have focused on the grafting of severed SDFT, but no ideal grafting material has been approved for clinical application. The present study aimed to evaluate the grafting of the midmetacarpal SDFT in donkeys with a double-layered polypropylene mesh clinically, ultrasonically, macro/microscopically. Clinically healthy adult donkeys (n = 24) were divided randomly and equally into two groups: control (C) and study (S) groups. Tenotomy was performed on all animals by excising a full-thickness segment from the mid-metacarpal SDFT. In the C group, a suture tenorrhaphy was performed. While in the S group, SDFT was grafted with the double-layered polypropylene mesh. The S group showed faster clinical improvements, and earlier appearance of normal newly formed tendinous tissue ultrasonically compared with the C group. Macroscopically, the grafted tendons showed a rapid restoration of normal color and consistency. Moreover, they had minimal peri-tendinous adhesions compared with the non-grafted tendons. Microscopically, the grafted tendons showed earlier vascularization and more mature collagen fibers compared with the non-grafted tendons. In the present study, we have succeeded to graft the gap of the mid-metacarpal SDFT of a donkey using the double-layered polypropylene mesh. This method is an economical way to accelerate the healing process of the SDFT and enhance the clinical recovery in donkeys.
The International journal of artificial organs, 2018
Decellularization of tissues can significantly improve regenerative medicine and tissue engineeri... more Decellularization of tissues can significantly improve regenerative medicine and tissue engineering by producing natural, less immunogenic, three-dimensional, acellular matrices with high biological activity for transplantation. Decellularized matrices retain specific critical components of native tissues such as stem cell niche, various growth factors, and the ability to regenerate in vivo. However, recellularization and functionalization of these matrices remain limited, highlighting the need to improve the characteristics of decellularized matrices. Incorporating nanoparticles into decellularized tissues can overcome these limitations because nanoparticles possess unique properties such as multifunctionality and can modify the surface of decellularized matrices with additional growth factors, which can be loaded onto the nanoparticles. Therefore, in this minireview, we highlight the various approaches used to improve decellularized matrices with incorporation of nanoparticles and...
Journal of biomedical materials research. Part A, Jan 22, 2018
Whole kidney decellularization is a promising approach in regenerative medicine for engineering a... more Whole kidney decellularization is a promising approach in regenerative medicine for engineering a functional organ. The reaction of the potential host depends on the biocompatibility of these decellularized constructs. Despite the proven ability of decellularized kidney scaffolds to guide cell attachment and growth, little is known about biocompatibility and hemocompatibility of these scaffolds. Our aim is to prepare decellularized kidneys of a clinically relevant size and evaluate its biocompatibility and hemocompatibility. Porcine kidneys were cannulated via the renal artery, and then perfused with 0.1% sodium dodecyl sulfate solution. Hematoxylin and eosin as well as DAPI staining confirmed cellular clearance from native kidneys in addition to preservation of the microstructure. SEM confirmed the absence of any cellular content within the scaffold, which is maintained in a well-organized 3D architecture. Decellularized kidneys retained the intact renal vasculature upon examinatio...
Artificial cells, nanomedicine, and biotechnology, Jan 27, 2018
No ideal cross-linking agent has been identified for decellularized livers (DLs) yet. In this stu... more No ideal cross-linking agent has been identified for decellularized livers (DLs) yet. In this study, we evaluated structural improvements and biocompatibility of porcine DLs after cross-linking with silver nanoparticles (AgNPs). Porcine liver slices were decellularized and then loaded with AgNPs (100 nm) after optimization of the highest non-toxic concentration (5 µg/mL) using Human hepatocellular carcinoma (HepG2) and EAhy926 human endothelial cell lines. The cross-linking effect of AgNPs was evaluated and compared to that of glutaraldehyde and ethyl carbodiimide hydrochloride and N-hydroxysuccinimide. The results indicated that AgNPs improved the ultra-structure of DLs' collagen fibres with good porosity and increased DLs' resistance against in vitro degradation with good cytocompatibility. AgNPs decreased the host inflammatory reaction against implanted porcine DL slices in vivo and increased the polarization of M2 macrophages. Thus, structural and functional improvements...
Jacquard weaving provides the opportunity to design complex pictorial and other patterning effect... more Jacquard weaving provides the opportunity to design complex pictorial and other patterning effects From the combination of warp and weft colors and /weaves. In the traditional fabric design process, the resultant visual perception of the design, using different colored yarns, can be attained only through the production of actual physical fabric sample, and this is every time consuming process. No truly accurate digital color methodology is yet available to assist designers in the initial development of product samples. Currently, there is very poor correlation between the color that is shown on the screen and the actual weave structure. On The following research we back to the nature as a main source of colors by using digital photos of several nature sources such as water, grass, clouds, sunrise, and sunset…..ECT. And by analyzing colors for this natural sources by the computer processes to implement color data base to be reference for the designer helping him to select warp and weft colors also the mix between warp and weft from several color verities and use the correct weaves to rich this color. Experimental work: The researchers select some patterns from nature sources to be an example for the application directed to jacquard upholstery fabrics. Colors selection:-Select warp colors. To select warp color from the data base established by the computer processses designer should select (X) parameters based on the weaving technique used for producing the upholstery fabric the number of warp colors should match with the fabric specification and according to the weave construction. Also the designer should specify the percentage of warp color appearance on the fabric buy presenting suggested specification to implement the correct fabric simulation in the warp direction.
The present experiment was performed to evaluate the autologous grafting of superficial digital f... more The present experiment was performed to evaluate the autologous grafting of superficial digital flexor tendon with cunean tendon in donkeys. It was carried out on 24 adult donkeys equally divided into 2 groups. Full thickness tenectomy with suturing leaving a gap of 2 cm of the mid- metacarpal segment of the SDFT was performed in the control group. Full thickness tenectomy followed by grafting with cunean tendon was performed in the second group. Both groups were evaluated at 6, 12 and 24 weeks P.O. No significant differences (P>0.05) was observed between the mean values of mid-metacarpal limb circumferences of both groups at all times of examination. The mean values of the dorsal fetlock angle showed non-significant differences (P>0.05) between both groups at all times of examination. At 12 weeks P.O., lameness was observed only in the control group. The sagittal gray-scale ultrasonic examinations showed that the grafted tendons had higher echogenicity than the control tendo...
Cells, 2021
The functionalization of decellularized scaffolds is still challenging because of the recellulari... more The functionalization of decellularized scaffolds is still challenging because of the recellularization-related limitations, including the finding of the most optimal kind of cell(s) and the best way to control their distribution within the scaffolds to generate native mimicking tissues. That is why researchers have been encouraged to study stem cells, in particular, mesenchymal stem cells (MSCs), as alternative cells to repopulate and functionalize the scaffolds properly. MSCs could be obtained from various sources and have therapeutic effects on a wide range of inflammatory/degenerative diseases. Therefore, in this mini-review, we will discuss the benefits using of MSCs for recellularization, the factors affecting their efficiency, and the drawbacks that may need to be overcome to generate bioengineered transplantable organs.
Journal of Biomedical Materials Research Part A, 2020
Acute liver failure (ALF) occurs due to severe liver damage that triggers rapid loss of normal li... more Acute liver failure (ALF) occurs due to severe liver damage that triggers rapid loss of normal liver function. Here, we investigate the usefulness of an injectable liver extracellular matrix (LECM)-rich hydrogel generated from an optimized decellularization protocol incorporated with silver nanoparticles (AgNPs) as a promising therapy for ALF. First, we optimized a non-destructive protocol for rat liver decellularization to obtain ECM-rich well-preserved scaffold. Then, LECM hydrogel generated from two commonly used decellularization protocols were compared by LECM hydrogel obtained from our optimized protocol. The ALF model was induced by an intraperitoneal (IP) thioacetamide (TAA) injection followed by the IP injection of LECM hydrogel, collagen-AgNPs mixture or LECM hydrogel-AgNPs mixture. LECM-rich scaffold and hydrogel were successfully obtained using our optimized decellularization protocol. Use of the LECM hydrogel-AgNPs mixture to treat TAA induced ALF, greatly improving liver injury and histological liver regeneration. Interleukin-6 (IL-6) and transforming growth factor beta ( TGF-β) expressions were significantly reduced, while albumin, hepatocyte growth factor, and Ki67-positive cells were highly expressed. Moreover, aspartate transaminase (AST) and alanine transaminase (ALT) plasma levels, and liver homogenate nitric oxide (NO) level were significantly lowered. In conclusion, the LECM hydrogel-AgNPs mixture has potential efficient therapeutic and regenerative effects on TAA-induced liver injury. This article is protected by copyright. All rights reserved.
Journal of Bioscience and Bioengineering, 2019
Comparative Clinical Pathology, 2019
Tendon injury is one of the most common causes of wastage in equine. The superficial digital flex... more Tendon injury is one of the most common causes of wastage in equine. The superficial digital flexor tendon (SDFT) gets affected the most, especially in the mid-metacarpal region. Several studies have focused on the grafting of severed SDFT, but no ideal grafting material has been approved for clinical application. The present study aimed to evaluate the grafting of the midmetacarpal SDFT in donkeys with a double-layered polypropylene mesh clinically, ultrasonically, macro/microscopically. Clinically healthy adult donkeys (n = 24) were divided randomly and equally into two groups: control (C) and study (S) groups. Tenotomy was performed on all animals by excising a full-thickness segment from the mid-metacarpal SDFT. In the C group, a suture tenorrhaphy was performed. While in the S group, SDFT was grafted with the double-layered polypropylene mesh. The S group showed faster clinical improvements, and earlier appearance of normal newly formed tendinous tissue ultrasonically compared with the C group. Macroscopically, the grafted tendons showed a rapid restoration of normal color and consistency. Moreover, they had minimal peri-tendinous adhesions compared with the non-grafted tendons. Microscopically, the grafted tendons showed earlier vascularization and more mature collagen fibers compared with the non-grafted tendons. In the present study, we have succeeded to graft the gap of the mid-metacarpal SDFT of a donkey using the double-layered polypropylene mesh. This method is an economical way to accelerate the healing process of the SDFT and enhance the clinical recovery in donkeys.
The International journal of artificial organs, 2018
Decellularization of tissues can significantly improve regenerative medicine and tissue engineeri... more Decellularization of tissues can significantly improve regenerative medicine and tissue engineering by producing natural, less immunogenic, three-dimensional, acellular matrices with high biological activity for transplantation. Decellularized matrices retain specific critical components of native tissues such as stem cell niche, various growth factors, and the ability to regenerate in vivo. However, recellularization and functionalization of these matrices remain limited, highlighting the need to improve the characteristics of decellularized matrices. Incorporating nanoparticles into decellularized tissues can overcome these limitations because nanoparticles possess unique properties such as multifunctionality and can modify the surface of decellularized matrices with additional growth factors, which can be loaded onto the nanoparticles. Therefore, in this minireview, we highlight the various approaches used to improve decellularized matrices with incorporation of nanoparticles and...
Journal of biomedical materials research. Part A, Jan 22, 2018
Whole kidney decellularization is a promising approach in regenerative medicine for engineering a... more Whole kidney decellularization is a promising approach in regenerative medicine for engineering a functional organ. The reaction of the potential host depends on the biocompatibility of these decellularized constructs. Despite the proven ability of decellularized kidney scaffolds to guide cell attachment and growth, little is known about biocompatibility and hemocompatibility of these scaffolds. Our aim is to prepare decellularized kidneys of a clinically relevant size and evaluate its biocompatibility and hemocompatibility. Porcine kidneys were cannulated via the renal artery, and then perfused with 0.1% sodium dodecyl sulfate solution. Hematoxylin and eosin as well as DAPI staining confirmed cellular clearance from native kidneys in addition to preservation of the microstructure. SEM confirmed the absence of any cellular content within the scaffold, which is maintained in a well-organized 3D architecture. Decellularized kidneys retained the intact renal vasculature upon examinatio...
Artificial cells, nanomedicine, and biotechnology, Jan 27, 2018
No ideal cross-linking agent has been identified for decellularized livers (DLs) yet. In this stu... more No ideal cross-linking agent has been identified for decellularized livers (DLs) yet. In this study, we evaluated structural improvements and biocompatibility of porcine DLs after cross-linking with silver nanoparticles (AgNPs). Porcine liver slices were decellularized and then loaded with AgNPs (100 nm) after optimization of the highest non-toxic concentration (5 µg/mL) using Human hepatocellular carcinoma (HepG2) and EAhy926 human endothelial cell lines. The cross-linking effect of AgNPs was evaluated and compared to that of glutaraldehyde and ethyl carbodiimide hydrochloride and N-hydroxysuccinimide. The results indicated that AgNPs improved the ultra-structure of DLs' collagen fibres with good porosity and increased DLs' resistance against in vitro degradation with good cytocompatibility. AgNPs decreased the host inflammatory reaction against implanted porcine DL slices in vivo and increased the polarization of M2 macrophages. Thus, structural and functional improvements...