Tarfena Amapu - Academia.edu (original) (raw)

Papers by Tarfena Amapu

Research paper thumbnail of Screening and Improvement of Local Isolates of Aspergillus Niger for Citric Acid Production

Bayero Journal of Pure and Applied Sciences, 2014

The study involved the screening of fourteen isolates of Aspergillus niger for citric acid produc... more The study involved the screening of fourteen isolates of Aspergillus niger for citric acid production from glucose. The study was aimed at screening and improving local strains of Aspergillus niger with potential for citric acid production. All the isolates screened produced varying amounts of citric acid, the highest was by the isolate designated CP3 which produced 12.81g/l and the least was B11 and O12 which produced 3.42g/l all after 6 days of submerged fermentation. The isolate CP3 was chosen for further studies. Strain improvement studies which was based on exposure of CP3 to ultraviolet irradiation for 10, 30 and 45 minutes increased citric acid yield to 22.20g/l (48.89% increase) by mutant M45 followed by a citric acid yield of 16.22g/l by the 30 minutes mutant (M30) and the least being M10 which produced 13.66g/l in all cases after 6 days of fermentation. The mutant (M45) also yielded higher amount of citric acid (19.64g/l) without methanol as additive compared to the parent strain (CP3) which gave a citric acid of 14.94g/l with the addition of methanol after six days (144h) of fermentation in both cases. Finding from this study showed that all the isolates have potential for citric acid production.

Research paper thumbnail of Potentials of Co-Culture of Saccharomyces Cerevisiae and Amylolytic Lactic Acid Bacteria on Cassava and Wheat Dough Fermentation

A THESIS SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES AHMADU BELLO UNIVERSITY, ZARIA, IN PARTI... more A THESIS SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES AHMADU BELLO UNIVERSITY, ZARIA, IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE AWARD OF DOCTOR OF PHILOSOPHY IN MICROBIOLOGY DEPARTMENT OF MICROBIOLOGY, FACULTY OF SCIENCE, AHMADU BELLO UNIVERSITY, ZARIA, NIGERIA.

Research paper thumbnail of Occurrence of Gastrointestinal Parasitic Associated with Exotic Dogs in Commercial Breeding Mills in Jos Metropolis-Nigeria

Academic Journal of Life Sciences

In Nigeria, there has been an increasing trend towards establishing puppy mills for commercial br... more In Nigeria, there has been an increasing trend towards establishing puppy mills for commercial breeding of exotic dogs. This study determined gastrointestinal parasites associated with exotic dogs of the existing puppy mills in Jos Metropolis Nigeria. A total of 150 dogs’ fresh feacal samples were collected from 25 different puppy mills and examined using formol ether and floatation concentration techniques. Data on risks factors variables of dogs’ age, number of dogs per kernel kennel type, nature of food and history of antiparasitic drugs usage were collected from the breeders using predesigned questionnaire. The result revealed an overall prevalence of 71.33% intestinal parasitic burden among the dogs. Chi square (χ2) analysis showed association (p< 0.05) between breeds of dogs and occurrence of parasites. The gastrointestinal parasites associated were most prevalent dogs in Neapolitan mastiff (86.36%) followed by Rottweiler (75.70%) and German Shepherd (57.14%) was the least ...

Research paper thumbnail of Antibiogram of Bacteria Isolated from Locally Processed Cow Milk Products Sold in Keffi Metropolis, Nasarawa State, Nigeria

Journal of Biology Agriculture and Healthcare, 2014

An investigation was conducted to determine the antibiogram of bacterial species isolated from lo... more An investigation was conducted to determine the antibiogram of bacterial species isolated from locally processed cow milk products sold in Keffi metropolis, Nasarawa state, Nigeria. Samples of the cow milk products, Raw milk, Nono, Kindrimo, and Manshanu, were each collected in triplicates from ten different sales locations in Keffi, and analyzed using standard bacteriological methods. Pour plate technique was employed for the isolation of bacteria from these cow milk products. The isolates were identified using cultural, morphological and biochemical methods, and thereafter the antibiotic susceptibility of each isolate was determined by modified Kirby Bauer diffusion method. Bacterial counts for Raw milk, Nono, Kindrimo and Manshanu were respectively in the range of 3.2×10 8-6.9×10 8 , 8.1× 10 8-2.70×10 9 , 7.3×10 8-9.8.×10 9 and 5.8×10 8-5.2×10 9. The bacterial species isolated were Staphylococcus aureus, Salmonella spp, Streptococcus spp. and E. coli with overall occurrence frequencies of 50%, 20%, 17.5% and 12.5%, respectively. E. coli was highly resistant to Septrin (80.2%), Sarfloxacin (75.3%) and Erythromycin (90.2%). Salmonella species was also highly resistant to Septrin (71.4%), Chloramphenicol (71.4%), Sparfloxacin (85.7%) and Erythromycin (71.4%). Similarly, Staphylococcus aureus was highly resistant to Septrin (75%), Chloramphenicol (75.4%), Sparfloxacin (87.5%), Amoxacillin (75.4%) and Augmentin (87.5%), while Streptococcus spp was highly resistant to Chloramphenicol (80.1%), Sparfloxacin (80.1%) and Augmentin (80%). These isolates may have developed resistance due to indiscriminate and frequent use of antibiotics which has now put the consumers of these milk products at risk of being infected with antibiotic resistant strains of pathogenic bacteria.

Research paper thumbnail of Screening and Improvement of Local Isolates of Aspergillus Niger for Citric Acid Production

Bayero Journal of Pure and Applied Sciences, 2014

The study involved the screening of fourteen isolates of Aspergillus niger for citric acid produc... more The study involved the screening of fourteen isolates of Aspergillus niger for citric acid production from glucose. The study was aimed at screening and improving local strains of Aspergillus niger with potential for citric acid production. All the isolates screened produced varying amounts of citric acid, the highest was by the isolate designated CP3 which produced 12.81g/l and the least was B11 and O12 which produced 3.42g/l all after 6 days of submerged fermentation. The isolate CP3 was chosen for further studies. Strain improvement studies which was based on exposure of CP3 to ultraviolet irradiation for 10, 30 and 45 minutes increased citric acid yield to 22.20g/l (48.89% increase) by mutant M45 followed by a citric acid yield of 16.22g/l by the 30 minutes mutant (M30) and the least being M10 which produced 13.66g/l in all cases after 6 days of fermentation. The mutant (M45) also yielded higher amount of citric acid (19.64g/l) without methanol as additive compared to the parent strain (CP3) which gave a citric acid of 14.94g/l with the addition of methanol after six days (144h) of fermentation in both cases. Finding from this study showed that all the isolates have potential for citric acid production.

Research paper thumbnail of Potentials of Co-Culture of Saccharomyces Cerevisiae and Amylolytic Lactic Acid Bacteria on Cassava and Wheat Dough Fermentation

A THESIS SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES AHMADU BELLO UNIVERSITY, ZARIA, IN PARTI... more A THESIS SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES AHMADU BELLO UNIVERSITY, ZARIA, IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE AWARD OF DOCTOR OF PHILOSOPHY IN MICROBIOLOGY DEPARTMENT OF MICROBIOLOGY, FACULTY OF SCIENCE, AHMADU BELLO UNIVERSITY, ZARIA, NIGERIA.

Research paper thumbnail of Occurrence of Gastrointestinal Parasitic Associated with Exotic Dogs in Commercial Breeding Mills in Jos Metropolis-Nigeria

Academic Journal of Life Sciences

In Nigeria, there has been an increasing trend towards establishing puppy mills for commercial br... more In Nigeria, there has been an increasing trend towards establishing puppy mills for commercial breeding of exotic dogs. This study determined gastrointestinal parasites associated with exotic dogs of the existing puppy mills in Jos Metropolis Nigeria. A total of 150 dogs’ fresh feacal samples were collected from 25 different puppy mills and examined using formol ether and floatation concentration techniques. Data on risks factors variables of dogs’ age, number of dogs per kernel kennel type, nature of food and history of antiparasitic drugs usage were collected from the breeders using predesigned questionnaire. The result revealed an overall prevalence of 71.33% intestinal parasitic burden among the dogs. Chi square (χ2) analysis showed association (p< 0.05) between breeds of dogs and occurrence of parasites. The gastrointestinal parasites associated were most prevalent dogs in Neapolitan mastiff (86.36%) followed by Rottweiler (75.70%) and German Shepherd (57.14%) was the least ...

Research paper thumbnail of Antibiogram of Bacteria Isolated from Locally Processed Cow Milk Products Sold in Keffi Metropolis, Nasarawa State, Nigeria

Journal of Biology Agriculture and Healthcare, 2014

An investigation was conducted to determine the antibiogram of bacterial species isolated from lo... more An investigation was conducted to determine the antibiogram of bacterial species isolated from locally processed cow milk products sold in Keffi metropolis, Nasarawa state, Nigeria. Samples of the cow milk products, Raw milk, Nono, Kindrimo, and Manshanu, were each collected in triplicates from ten different sales locations in Keffi, and analyzed using standard bacteriological methods. Pour plate technique was employed for the isolation of bacteria from these cow milk products. The isolates were identified using cultural, morphological and biochemical methods, and thereafter the antibiotic susceptibility of each isolate was determined by modified Kirby Bauer diffusion method. Bacterial counts for Raw milk, Nono, Kindrimo and Manshanu were respectively in the range of 3.2×10 8-6.9×10 8 , 8.1× 10 8-2.70×10 9 , 7.3×10 8-9.8.×10 9 and 5.8×10 8-5.2×10 9. The bacterial species isolated were Staphylococcus aureus, Salmonella spp, Streptococcus spp. and E. coli with overall occurrence frequencies of 50%, 20%, 17.5% and 12.5%, respectively. E. coli was highly resistant to Septrin (80.2%), Sarfloxacin (75.3%) and Erythromycin (90.2%). Salmonella species was also highly resistant to Septrin (71.4%), Chloramphenicol (71.4%), Sparfloxacin (85.7%) and Erythromycin (71.4%). Similarly, Staphylococcus aureus was highly resistant to Septrin (75%), Chloramphenicol (75.4%), Sparfloxacin (87.5%), Amoxacillin (75.4%) and Augmentin (87.5%), while Streptococcus spp was highly resistant to Chloramphenicol (80.1%), Sparfloxacin (80.1%) and Augmentin (80%). These isolates may have developed resistance due to indiscriminate and frequent use of antibiotics which has now put the consumers of these milk products at risk of being infected with antibiotic resistant strains of pathogenic bacteria.