Tatiana C.Coutinho - Academia.edu (original) (raw)

Papers by Tatiana C.Coutinho

PLOS Genetics, 2011

Sclerotinia sclerotiorum and Botrytis cinerea are closely related necrotrophic plant pathogenic f... more Sclerotinia sclerotiorum and Botrytis cinerea are closely related necrotrophic plant pathogenic fungi notable for their wide host ranges and environmental persistence. These attributes have made these species models for understanding the complexity of necrotrophic, broad host-range pathogenicity. Despite their similarities, the two species differ in mating behaviour and the ability to produce asexual spores. We have sequenced the genomes of one strain of S. sclerotiorum and two strains of B. cinerea. The comparative analysis of these genomes relative to one another and to other sequenced fungal genomes is provided here. Their 38-39 Mb genomes include 11,860-14,270 predicted genes, which share 83% amino acid identity on average between the two species. We have mapped the S. sclerotiorum assembly to 16 chromosomes and found large-scale co-linearity with the B. cinerea genomes. Seven percent of the S. sclerotiorum genome comprises transposable elements compared to ,1% of B. cinerea. The arsenal of genes associated with necrotrophic processes is similar between the species, including genes involved in plant cell wall degradation and oxalic acid production. Analysis of secondary metabolism gene clusters revealed an expansion in number and diversity of B. cinerea-specific secondary metabolites relative to S. sclerotiorum. The potential diversity in secondary metabolism might be involved in adaptation to specific ecological niches. Comparative genome analysis revealed the basis of differing sexual mating compatibility systems between S. sclerotiorum and B. cinerea. The organization of the mating-type loci differs, and their structures provide evidence for the evolution of heterothallism from homothallism. These data shed light on the evolutionary and mechanistic bases of the genetically complex traits of necrotrophic pathogenicity and sexual mating. This resource should facilitate the functional studies designed to better understand what makes these fungi such successful and persistent pathogens of agronomic crops.

Appetite, 2008

We aimed to assess whether modulation of the dorsolateral prefrontal cortex (DLFPC) with noninvas... more We aimed to assess whether modulation of the dorsolateral prefrontal cortex (DLFPC) with noninvasive brain stimulation, namely transcranial direct current stimulation (tDCS), modifies food craving in healthy subjects. We performed a randomized sham-controlled cross-over study in which 23 subjects received sham and active tDCS (anode left/cathode right and anode right/cathode left) of the DLPFC. Subjects were exposed to food and also watched a movie of food associated with strong craving. Desire for food consumption was evaluated by visual analogue scales (VAS) and food consumption before and after treatment. In addition we measured visual attention to food using an eye tracking system. Craving for viewed foods as indexed by VAS was reduced by anode right/cathode left tDCS. After sham stimulation, exposure to real food or food-related movie increased craving; whereas after anode left/cathode right tDCS, the food-related stimuli did not increase craving levels, as revealed by the VAS scale. Moreover, compared with sham stimulation, subjects fixated food-related pictures less frequently after anode right/cathode left tDCS and consumed less food after both active stimulation conditions. These changes were not related to mood changes after any type of tDCS treatment. The effects of tDCS on food craving might be related to a modulation of neural circuits associated with reward and decision-making. #

Genetics and Molecular Biology, 2001

Since the isolation of the first glycine-rich proteins (GRPs) in plants a wealth of new GRPs have... more Since the isolation of the first glycine-rich proteins (GRPs) in plants a wealth of new GRPs have been identified. The highly specific but diverse expression pattern of grp genes, taken together with the distinct sub-cellular localization of some GRP groups, clearly indicate that these proteins are involved in several independent physiological processes. Notwithstanding the absence of a clear definition of the role of GRPs in plant cells, studies conducted with these proteins have provided new and interesting insights into the molecular biology and cell biology of plants. Complexly regulated promoters and distinct mechanisms for the regulation of gene expression have been demonstrated and new protein targeting pathways, as well as the exportation of GRPs from different cell types have been discovered. These data show that GRPs can be useful as markers and/or models to understand distinct aspects of plant biology. In this paper, the structural and functional features of these proteins in sugarcane (Saccharum officinarum L.) are summarized. Since this is the first description of GRPs in sugarcane, special emphasis has been given to the expression pattern of these GRP genes by studying their abundance and prevalence in the different cDNA-libraries of the Sugarcane Expressed Sequence Tag (SUCEST) project . The comparison of sugarcane GRPs with GRPs from other species is also discussed.

BACKGROUND: To establish a prospective study to verify the correlation between the cytopathologic... more BACKGROUND: To establish a prospective study to verify the correlation between the cytopathologic diagnosis obtained through FNA and the hystopathologic diagnosis of the lesions from the maxillofacial region of patients at the Buco-Maxilofacial Surgery and Traumatology Service. METHOD: Analysis of the results from cytologic and histopathological diagnosis issued by the Pathologic Anatomy Service of the Antônio Pedro University Hospital (UFF, Niterói-RJ, Brazil). Forty-five patient who underwent FNA followed by incisional or excisional biopsy, were selected. Cases in which information was incomplete were excluded. RESULTS: The total number of cases selected was 45. The diagnostic accuracy obtained was of 77.8% (35) in FNA cytopathology, with a 100% in specificity and sensibility. CONCLUSION: The cytopathology performed through FNA is an efficient initial diagnostic method, with low cost, painless, and quickly accomplished, which helps the identification of the lesion's nature and allows the professional a more accurate evaluation for the surgical or therapeutic approach.

Este artigo tem por objetivo apresentar o perfil dos conselheiros de quatro conselhos setoriais d... more Este artigo tem por objetivo apresentar o perfil dos conselheiros de quatro conselhos setoriais do Município de Santo André com a finalidade de compreender de que forma fatores como idade, escolaridade, gênero, entre outros, podem influenciar positiva ou negativamente na representividade destes Conselhos. Para isso, foram realizadas entrevistas e feita análise de documentos internos e atas das reuniões dos conselhos. Os resultados demonstram que, embora existam diferenças no que se refere à escolaridade dos representantes da sociedade civil e do poder público que poderiam refletir uma correlação de forças desigual dentro dos conselhos, os representantes da sociedade civil possuem forte liderança, o que facilita o retorno das informações à sociedade e vice-versa. Conclui-se que, para que os conselhos locais tornem-se um canal de participação mais equilibrado, é preciso investir na capacitação dos conselheiros e em mecanismos de incentivo à participação de jovens.

International Journal of Gynecology & Obstetrics, 2009

Planta, 2007

The glycine-rich protein AtGRP2 is one of the four members of the cold-shock domain (CSD) protein... more The glycine-rich protein AtGRP2 is one of the four members of the cold-shock domain (CSD) protein family in Arabidopsis. It is characterized by the presence of a nucleic acid-binding CSD domain, two glycine-rich domains and two CCHC zinc-fingers present in nucleic acid-binding proteins. In an attempt to further understand the role of CSD/GRP proteins in plants, we have proceeded to the functional characterization of the AtGRP2 gene. Here, we demonstrate that AtGRP2 is a nucleo-cytoplasmic protein involved in Arabidopsis development with a possible function in cold-response. Expression analysis revealed that the AtGRP2 gene is active in meristematic tissues, being modulated during flower development. Down-regulation of AtGRP2 gene, using gene-silencing techniques resulted in early flowering, altered stamen number and affected seed development. A possible role of AtGRP2 as an RNA chaperone is discussed.

Revista Da Sociedade Brasileira De Fonoaudiologia, 2009

This research had the aim to identify adaptations on the stomatognathic system of individuals wit... more This research had the aim to identify adaptations on the stomatognathic system of individuals with maxillomandibular disproportions. The literature review was carried out using national and international databases, addressing adaptations on the stomatognathic system of subjects with maxillomandibular disproportions submitted to orthognathic surgery associated to speech-language intervention. It was found that subjects with prognathism and retrognathism present adaptations in all oral-motor functions during the presurgical period. In mandibular prognathism, the most adapted function is mastication, which is characterized by vertical movements using the dorsum of the tongue for kneading the food, and less use of the masticatory muscles. In mandibular retrognathism, studies showed that deglutition is most adapted, being characterized by anterior mandibular slide, in addition to postero-anterior movement of the tongue and perioral muscles activation. After orthognathic surgery, some cases may present adequacy of the stomatognathic system functions. In other, however, adaptations may persist, making it necessary that speech-language therapists are well informed about pre-existent adaptations, in order to plan the best rehabilitation process for each case.

OBJECTIVES: to perform a systematic literature review through a careful survey of scientific arti... more OBJECTIVES: to perform a systematic literature review through a careful survey of scientific articles investigating serial casting effects in children clinically diagnosed with spastic cerebral palsy. METHODS: systematic literature survey performed during the period of March to August 2004, in Medline, Lilacs and PEDro electronic databases published in English and Portuguese from 1980 to 2004. The evidence level of the articles studied was rated according to the Magee scale. RESULTS: the four articles included in this review met all the inclusion criteria and were rated poor according to the Magee scale (agreement index Kappa K = 1.0) due to the methodological limitations determined. CONCLUSIONS: the evidence used in this systematic review was insufficient to support the use of serial casting in children with cerebral palsy. Further research is required to fulfill the need of higher methodological standards to validate evidence based practice.

Psicologia Em Estudo, 2005

The objective of this study was to adapt The Socially Responsible Consumer Behavior Scale ECSRC ... more The objective of this study was to adapt The Socially Responsible Consumer Behavior Scale ECSRC (Roberts, 1996) to Brazil. The main objective of this scale is to evaluate to what degree consumers who tend to express conduct which indicates social responsibility take this conduct into consideration at the moment of product acquisition. Two hundred ninety-eight university students (average 21 yrs old, d.p. = 4.60, 72.4% female) participated in this study. Three factors, which explain 41.1% of the total variance were found through an analysis of the principal axes (PAF) with a Promax rotation. The first factor grouped five items together and was called environmental concern (a = 0.90), the second, concern about recycling, was composed of four items (a = 0.75) and, finally, six items from the scale were grouped together in the third factor called social concern (a = 0.70). These results are coherent with the conception that multiple factors are necessary to evaluate consumer behavior, and also, suggest the adequacy of the ECSRC to the Brazilian context.

Obesity Surgery, 2008

Postprandial hypoglycemia is a common complication of bariatric surgery. It is usually caused by ... more Postprandial hypoglycemia is a common complication of bariatric surgery. It is usually caused by late dumping syndrome, but a few other causes have already been described, including insulinoma and noninsulinoma pancreatogenous hypoglycemic syndrome (NIPHS). Considering that NIPHS is a recently described syndrome and is also very rare, therapeutic approaches are still not consensual. We report the case of a 26-year-old woman who was submitted to bariatric surgery and presented episodic postprandial hypoglycemic episodes after 16 months. Fasting C-peptide, insulin, and glucose were normal. Because of the possibility of NIPHS, clinical treatment was initiated with verapamil and acarbose, leading to a significant reduction of hypoglycemic episodes and also their severity. Surgery is the most common approach to NIPHS. However, in cases of mild or moderate symptoms, it is important to consider the possibility of pharmacological treatment. This approach may result, at least for some time, in an amelioration of symptoms without the need of an aggressive procedure.

New promising assessment possibilities have been studied in Pervasive Developmental Disorders (PD... more New promising assessment possibilities have been studied in Pervasive Developmental Disorders (PDD) such as eye tracking analysis. This study compares the perception of human faces in children with PDD and children with typical development through the analysis of eye-tracking patterns. Ten participants with PDD, mean age of 11.9 years (SD=3.22), were matched by age and gender with 10 participants with typical development. Ten neutral faces, in black-and-white, were observed per 10 seconds each while some stimuli were controlled: gender (male or female); position (normal or upside-down); presence of eyes (present or absent). Significant differences were found: PDD participants spent less time looking at eyes of male faces; spent less time with faces and eyes in upside-down figures and spent less time looking at faces with eyes and faces with occulted eyes; and control group spent more time looking at the eyes region when these were occulted.