Terezinha Knöbl - Academia.edu (original) (raw)

Papers by Terezinha Knöbl

Revista de Educação Continuada em Medicina Veterinária e Zootecnia do CRMV-SP, Jun 3, 2016

Arquivos do Instituto Biológico, Mar 1, 2005

Influência da biossegurança na colonização intestinal por Escherichia coli em psitacídeos.

animal - open space, Dec 1, 2023

Brazilian Journal of Veterinary Research and Animal Science, Dec 7, 2008

RESUMO.-[Caracterização molecular de fatores de virulência em Aeromonas hydrophila obtidas de pei... more RESUMO.-[Caracterização molecular de fatores de virulência em Aeromonas hydrophila obtidas de peixes.] Múltiplos fatores podem estar envolvidos nos processos de virulência de Aeromonas hydrophila. O objetivo do presente trabalho foi veriϐicar a presença dos genes de virulência aerolisina, hidrolipase, elastase e lipase, utilizando a reação em cadeia da polimerase (PCR), em isolados de Aeromonas hydrophila obtidos de peixes do Vale do São Francisco e, avaliar sua virulência de acordo com a presença desses genes de virulência em alevinos de tilápia do Nilo. Cento e

A Escherichia coli extra-intestinal (ExPEC) e uma classe heterogenea de patogenos envolvidos em i... more A Escherichia coli extra-intestinal (ExPEC) e uma classe heterogenea de patogenos envolvidos em inumeros processos morbidos em humanos e animais, que se subdivide em E. coli uropatogenica (UPEC), E. coli causadora de meningite neonatal (NMEC) e E. coli patogenica para aves (APEC) [1]. As APEC tem grande importância na industria de producao de aves por ocasionarem perdas economicas que se estendem dos incubatorios ao abate, estando presentes em todas as fases da cadeia de producao de aves. Nos ultimos anos, o potencial zoonotico dessa classe tem sido alvo frequente dos trabalhos realizados com APEC. O perfil de resistencia a multiplas drogas exibido por amostras aviarias devido a presenca de elementos geneticos moveis como plasmideos e integrons tambem tem sido explorado [2, 3]. Alguns clones do patotipo aviario, principalmente os pertencentes ao grupo filogenetico B2, sao muitos similares a estirpes das ExPEC que afetam humanos (UPEC e NMEC), sendo indistinguiveis genotipica e fenotipicamente [1, 2]. As aves de producao tem sido consideradas como reservatorio de ExPEC e o consumo de carne de aves pode assumir um importante papel na cadeia epidemiologica das infeccoes nos seres humanos [1–3]. Recentemente, a hipotese de que infeccoes do trato urinario podem ser consideradas doencas veiculadas por alimentos de origem animal ganhou forca, uma vez que bacterias presentes nesses alimentos sao capazes de colonizar o intestino humano. Em situacoes especificas, essas bacterias podem provocar infeccoes em sitios distantes, incluindo vesicula urinaria e rins [3]. Levando em conta essas evidencias, o presente trabalho caracterizou amostras APEC do grupo filogenetico B2 em relacao a resistencia aos antimicrobianos e virulencia.

Journal of Applied Animal Research, 2020

Artificial insemination is the foundation of Brazilian intensive pig farming and the semen qualit... more Artificial insemination is the foundation of Brazilian intensive pig farming and the semen quality is a key point for the success of the productive chain. Several bacteria have already been reported as semen contaminants and may cause morphological and functional changes in sperm, decreasing male fertility, in addition to predisposing females to reproductive failures. The objective of this study was to perform a bacteriological examination of boar semen (Sus scrofa) obtained from an Artificial Insemination Centre in the southwestern region of Paraná. One hundred semen samples were assessed for volume, colour, smell, motility, agglutinated sperm cells and for bacterial contamination. Bacterial species were further identified by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. The results revealed positive bacterial isolation in 43% of the samples, with predominance of Gram-negative pathogens. The identified species were: Pseudomonas aeruginosa, Proteus mirabilis, Escherichia coli, Kerstersia gyiorum, Aerococcus viridans, Brevibacterium casei, Providencia stuartii, Citrobacter koseri and Staphylococcus pasteuri. The E. coli contamination was associated with decreased sperm motility (p < 0.01) and vigour (p = 0.002). Despite the frequent usage, the antimicrobial resistance tests showed that few isolates were resistant to gentamycin or neomycin, drugs that are commonly used to extend semen viability in Brazil.

Veterinary Microbiology, Apr 1, 2022

Extraintestinal pathogenic Escherichia coli (ExPEC) is a globally distributed pathogen, with urop... more Extraintestinal pathogenic Escherichia coli (ExPEC) is a globally distributed pathogen, with uropathogenic E. coli (UPEC) and sepsis-associated E. coli (SEPEC) pathotypes particularly involved in human and companion animal disease, while avian pathogenic pathotype (APEC) severely impact poultry health and production. Similarities between APEC from poultry/meat and human ExPEC suggest that some APEC lineages may have zoonotic potential. ExPEC sequence type 73 (ST73) and its clonal complex (CC) are increasing causes of urinary tract infections and sepsis, but its role in zoonotic disease is less well understood. Here, we analyzed the genome sequences of 25 E. coli isolates from Brazil (11 APEC and 14 UPEC) from two time periods, from poultry producing areas and hospitals in the same geographical regions. Isolates were compared to 558 publicly available ST73/CC73 global sequences. Brazilian APEC harbored virulence factors associated with UPEC/SEPEC such as sfa, cnf1, vat, usp, hlyA, iron acquisition and protectins/serum resistance systems, while lacking some common APEC markers and widespread multidrug resistance. Analysis of core genome MLST and SNP phylogenetic trees indicated evolutionary relationships between subgroups of the Brazilian APEC to two contemporary Brazilian UPEC isolates from the same region, and one Brazilian UPEC available from another study. Phylogenies showed a non-host, geographical, or pathotype specificity, with APEC isolates clustering closely with international human UPEC, SEPEC. The remaining Brazilian UPEC grouped within human clusters. Collectively, this suggests a zoonotic potential for subgroups of Brazilian APEC from the ST73 lineage that could contaminate poultry products and subsequently cause human infection.

Antibiotics, Feb 1, 2023

This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY

Brazilian Journal of Poultry Science, Apr 1, 2018

Raoultella ornithinolytica is a gram-negative aerobic bacterium belonging to Enterobacteriaceae f... more Raoultella ornithinolytica is a gram-negative aerobic bacterium belonging to Enterobacteriaceae family, an emerging pathogen that causes several pathogenic conditions in man, with little veterinary importance; however, its identification is underestimated by conventional laboratory techniques. The present study reports the identification of R. ornithinolytica in Tinamus solitaries, during a routine sanitary evaluation of aerobic enterobacteria in cloacal microbiota of birds belonging to the Güira Oga Center, Puerto Iguazu, Argentina. The sample was preliminary classified as Klebsiella spp.; however, after the use of the MALDI-TOF MS technique it was identified as R. ornithinolytica. The sample was submitted to an antimicrobial susceptibility test, where it showed a similar pattern profile as reported in the literature, with resistance to ampicillin and other β-lactam antibiotics. It is possible that Raoultella spp are more common in birds as it is reported. Therefore, review studies on bacteria collections of avian origin, as well as cases with confirmation of Klebsiella, should be deeply evaluated in laboratorial routine, mainly due to the pathogenic potential of R. ornithinolytica for Poultry, as well as for public health.

No Brasil sao encontradas mais de 80 especies de aves da familia Psittacidae, incluindo papagaios... more No Brasil sao encontradas mais de 80 especies de aves da familia Psittacidae, incluindo papagaios, araras e periquitos. A manutencao destas aves em zoologicos contribui para a conservacao das especies, mas o ambiente de cativeiro pode favorecer a colonizacao intestinal por enterobacterias. O objetivo deste trabalho foi identificar a presenca de Klebsiella spp . nas fezes de psitacideos cativos. Foram analisadas 34 amostras de fezes. Destas, 11 (32,35%) foram positivas para Klebsiella spp., com identificacao das especies: K. Pneumoniae e K. oxytoca . Os resultados demonstraram uma elevada frequencia de aves colonizadas por Klebsiella spp. Novos estudos sao necessarios para determinacao da patogenicidade destas estirpes, perfil de resistencia antimicrobiana e risco zoonotico.

The amount of antibiotics consumed by food-producing animals around the world is more than that u... more The amount of antibiotics consumed by food-producing animals around the world is more than that used by humans, according to some estimates. Transference of plasmids is a key mechanism for the spread of antibiotic resistance genes by horizontal gene transfer. Ceftiofur is a veterinary antibiotic of third generation cephalosporin class widely use in food-producing animals worldwide. The aim of the present study was to investigate the prevalence of plasmidmediated AmpC determinants among ceftiofur-resistant E.coli isolates from chickens. A collection of 158 isolates, 80 isolates of avian pathogenic E. coli (APEC) and 78 isolates of fecal E. coli of health broilers and laying hens from four States (SP, MG, PR, RS) were screened for ceftiofur resistance. 48 strains that showed MIC ≥ 8 mg/L for ceftiofur was investigated to presence of plasmid-mediated AmpC β-lactamases (DHA, ACC, CMY) through PCR. Presence of mobilizing elements (ISCR1, ISEcp1, IS26) was determined by PCR. Resistance ph...

Journal of Medical Primatology, 2019

Non-human primates are susceptible to many bacteria, some of which bear zoonotic potential. We re... more Non-human primates are susceptible to many bacteria, some of which bear zoonotic potential. We report the pathologic features of spontaneous fulminating meningoencephalitis by Staphylococcus aureus in a captive infant golden-headed lion tamarin (Leontopithecus chrysomelas) from Brazil.

Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], Jan 2, 2017

Psittacine birds have been identified as reservoirs of diarrheagenic Escherichia coli, a subset o... more Psittacine birds have been identified as reservoirs of diarrheagenic Escherichia coli, a subset of pathogens associated with mortality of children in tropical countries. The role of other orders of birds as source of infection is unclear. The aim of this study was to perform the molecular diagnosis of infection with diarrheagenic E. coli in 10 different orders of captive wild birds in the state of São Paulo, Brazil. Fecal samples were analyzed from 516 birds belonging to 10 orders: Accipitriformes, Anseriformes, Columbiformes, Falconiformes, Galliformes, Passeriformes, Pelecaniformes, Piciformes, Psittaciformes and Strigiformes. After isolation, 401 E. coli strains were subjected to multiplex PCR system with amplification of genes eae and bfp (EPEC), stx1 and stx2 for STEC. The results of these tests revealed 23/401 (5.74%) positive strains for eae gene, 16/401 positive strains for the bfp gene (3.99%) and 3/401 positive for stx2 gene (0.75%) distributed among the orders of Psittaci...

Brazilian Journal of Veterinary Research and Animal Science

The role of psittacine birds as a reservoir of avian pathogenic Escherichia coli (APEC) is not kn... more The role of psittacine birds as a reservoir of avian pathogenic Escherichia coli (APEC) is not known but would be helpful in understanding the human – animal interface, since the enteric microbiota of these birds consists of Gram positive bacteria. The aim of this study was to identify the presence of APEC in feces of clinically healthy Guaruba guarouba. To do this, we isolated and analyzed E. coli from cloacal fecal samples taken from 87 psittacine birds from six zoologic parks, three commercial breeders and one conservation breeder. Of the 87 birds examined, 46 (52.87%) presented E. coli in feces. The presence of the following eight virulence genes was determined by the polymerase chain reaction (PCR): irp2, iucD, iss, vat, cvi/cva, tsh, astA, and papC, and 29 (63.04%) of 46 E. coli isolates tested were positive at least one of the eight genes studied. The frequency of virulence genes observed in isolates of E. coli were 32.6% (15/46) irp2, 26% (12/46) iucD, 19.5% iss (9/46), 17.4...