Thais Sales - Academia.edu (original) (raw)

Papers by Thais Sales

Journal of Cellular Biochemistry, 2019

A large number of natural compounds, such as phenolic compounds, have been scientifically evaluat... more A large number of natural compounds, such as phenolic compounds, have been scientifically evaluated in the search for enzyme inhibitors. The interactions between the phenolic compound p-coumaric acid and the enzymes present in snake venoms (used as research tools) were evaluated in vitro and in silico. The p-coumaric acid was able to inhibit 31% of the phospholipase activity induced by Bothrops alternatus venom, 27% of the hemolytic activity induced by B. moojeni, 62.5% of the thrombolytic activity induced by B. jararacussu, and approximately 27% of the activity thrombosis induced by Crotalus durissus terrificus. Previous incubation of p-coumaric acid with the venoms of B. atrox and B. jararacussu increased the coagulation time by 2.18 and 2.16-fold, respectively. The activity of serine proteases in B. atrox and B. jararacussu venoms was reduced by 60% and 66.34%, respectively. Computational chemistry analyses suggests the specific binding of p-coumaric acid to the active site of proteases through hydrogen and hydrophobic interactions. The phenolic compound evaluated in this work has great potential in therapeutic use to both prevent and treat hemostatic alterations, because the venom proteins inhibited by the p-coumaric acid have high homology with human proteins that have a fundamental role in several pathologies. K E Y W O R D S enzyme inhibitors, phenolic compound, phospholipases A 2 , proteases 1 | INTRODUCTION Snakebite accidents are considered public health problems and were included by the World Health Organization on the list of neglected tropical diseases only in 2009, equating their severity with tropical diseases such as dengue fever, malaria, and hemorrhagic fever. 1 Underestimated numbers indicate that 1.2 to 5.5 million people are bitten by snakes annually, with a higher incidence in South and SouthEast Asia, Sub-Saharan Africa, Central America, and South America. From those accidents, 25 000 to 125 000 results in deaths and approximately 400 000 survive with permanent sequelae. 2,3 Snakes of the Viperidae family are responsible for most of the accidents in the Americas. They have pathophysiological characteristics such as the alterations associated with hemorrhages, necrosis, edema formation, hypovolemia, coagulopathy, and cardiogenic shock. 4 This large number of clinical complications is a result of the action of several components present in the venom, with

Journal of Cellular Biochemistry, 2019

A large number of natural compounds, such as phenolic compounds, have been scientifically evaluat... more A large number of natural compounds, such as phenolic compounds, have been scientifically evaluated in the search for enzyme inhibitors. The interactions between the phenolic compound p-coumaric acid and the enzymes present in snake venoms (used as research tools) were evaluated in vitro and in silico. The p-coumaric acid was able to inhibit 31% of the phospholipase activity induced by Bothrops alternatus venom, 27% of the hemolytic activity induced by B. moojeni, 62.5% of the thrombolytic activity induced by B. jararacussu, and approximately 27% of the activity thrombosis induced by Crotalus durissus terrificus. Previous incubation of p-coumaric acid with the venoms of B. atrox and B. jararacussu increased the coagulation time by 2.18 and 2.16-fold, respectively. The activity of serine proteases in B. atrox and B. jararacussu venoms was reduced by 60% and 66.34%, respectively. Computational chemistry analyses suggests the specific binding of p-coumaric acid to the active site of proteases through hydrogen and hydrophobic interactions. The phenolic compound evaluated in this work has great potential in therapeutic use to both prevent and treat hemostatic alterations, because the venom proteins inhibited by the p-coumaric acid have high homology with human proteins that have a fundamental role in several pathologies. K E Y W O R D S enzyme inhibitors, phenolic compound, phospholipases A 2 , proteases 1 | INTRODUCTION Snakebite accidents are considered public health problems and were included by the World Health Organization on the list of neglected tropical diseases only in 2009, equating their severity with tropical diseases such as dengue fever, malaria, and hemorrhagic fever. 1 Underestimated numbers indicate that 1.2 to 5.5 million people are bitten by snakes annually, with a higher incidence in South and SouthEast Asia, Sub-Saharan Africa, Central America, and South America. From those accidents, 25 000 to 125 000 results in deaths and approximately 400 000 survive with permanent sequelae. 2,3 Snakes of the Viperidae family are responsible for most of the accidents in the Americas. They have pathophysiological characteristics such as the alterations associated with hemorrhages, necrosis, edema formation, hypovolemia, coagulopathy, and cardiogenic shock. 4 This large number of clinical complications is a result of the action of several components present in the venom, with