Thiago Cezar Fujita - Academia.edu (original) (raw)
Papers by Thiago Cezar Fujita
In the present study, nasal mucus from patients with leprosy were analyzed by PCR using specific ... more In the present study, nasal mucus from patients with leprosy were analyzed by PCR using specific primers for Lsr2 gene of Mycobacterium leprae.
Anticancer research, 2016
The WT1 gene encodes a transcription factor involved in regulation of many cellular processes, in... more The WT1 gene encodes a transcription factor involved in regulation of many cellular processes, including proliferation, differentiation, mRNA processing and apoptosis, besides acting as a transcription repressor of growth factors and their receptors' genes. This gene is expressed at high levels in several types of cancers, including acute leukemias. In this regard, many studies have identified WT1 protein as a tumor antigen, considered a target molecule for clinical application in human acute leukemias. Immunotherapy using WT1 antigen has been effective in stimulating immune responses against leukemic cells. Regarding adoptive immunotherapy, the use of dendritic cells (DCs) for the WT1-specific cytotoxic T cells generation proved to be efficient in the development and maintenance of immunologic cells. Therefore, these therapeutic methods, that provided enthusiasm for moving ahead, highlight several opportunities and challenges to be used in clinical practice for managing acute l...
Este trabalho teve o objetivo de analisar os casos de neoplasias atendidos no Hospital de Câncer ... more Este trabalho teve o objetivo de analisar os casos de neoplasias atendidos no Hospital de Câncer de Londrina (HCL) no ano de 2005, classificando-os de acordo com a Lista de Neoplasias Relacionadas ao Trabalho (PORTARIA 1339/99) para verificar a frequencia de neoplasias que constam da referida lista. Foi realizado um estudo transversal de casos de pacientes residentes em municipios pertencentes a 17a Regional de Saude do Parana, inscritos no Hospital do Câncer de Londrina (HCL). Os dados foram coletados por fonte secundaria, por meio do Registro Hospitalar de Câncer disponivel na instituicao em estudo. Foram selecionados os casos de pacientes conforme dados acerca do tipo de câncer e ocupacao. Dos 784 casos inscritos no servico, 296 casos faziam parte da Lista de Neoplasias Relacionadas ao Trabalho. Os tres tipos de câncer mais frequentes que constavam da lista, foram as neoplasias malignas de pele naomelanoma, seguidas das neoplasias de estomago, bronquios e pulmao. As ocupacoes for...
Chronic myeloid leukemia (CML) is a malignant myeloproliferative disorder that originates from a ... more Chronic myeloid leukemia (CML) is a malignant myeloproliferative disorder that originates from a pluripotent stem cell characterized by abnormal release of the expanded, malignant stem cell clone from the bone marrow into the bloodstream. The vast majority of patients with CML present Bcr-Abl transcripts. Lactate dehydrogenase (LDH) is considered a biochemical marker common for tumor growth, anaerobic glycolysis and has been considered a poor prognostic factor for acute myeloid leukemia. Therefore, this study aimed to evaluate the concentration of LDH in plasma and the detection of the Bcr-Abl transcripts in patients with CML and healthy donors. We analyzed 22 patients demonstrably diagnosed with CML and 56 healthy donors. LDH concentration in plasma was higher in patients with CML. All patients with CML in this study were under treatment, but even so four patients had the Bcr-Abl (b3a2) transcript in peripheral blood. Two out of the four patients with b3a2 showed higher LDH (486 U ...
Molecular Biology Reports
Acute lymphoid leukemia (ALL) is a type of hematological neoplasm that affects the precursor cell... more Acute lymphoid leukemia (ALL) is a type of hematological neoplasm that affects the precursor cells of strains B, T and NK, with a higher incidence in the pediatric range. The pathophysiology of ALL is characterized by chromosomal abnormalities and genetic alterations involved in the differentiation and proliferation of lymphoid precursor cells. Despite the lack of information in the literature, it is believed that leukemogenesis originates from a complex interaction between environmental and genetic factors, which combined lead to cellular modifications. Environmental factors have been evaluated as possible predisposing factors in the development of ALL but there are still conflicting results in the world literature. In this context, the aim of the present review is to discuss the major exogenous factors regarding ALL.
International Journal of Cancer Therapy and Oncology
Anticancer research, 2016
The WT1 gene encodes a transcription factor involved in regulation of many cellular processes, in... more The WT1 gene encodes a transcription factor involved in regulation of many cellular processes, including proliferation, differentiation, mRNA processing and apoptosis, besides acting as a transcription repressor of growth factors and their receptors' genes. This gene is expressed at high levels in several types of cancers, including acute leukemias. In this regard, many studies have identified WT1 protein as a tumor antigen, considered a target molecule for clinical application in human acute leukemias. Immunotherapy using WT1 antigen has been effective in stimulating immune responses against leukemic cells. Regarding adoptive immunotherapy, the use of dendritic cells (DCs) for the WT1-specific cytotoxic T cells generation proved to be efficient in the development and maintenance of immunologic cells. Therefore, these therapeutic methods, that provided enthusiasm for moving ahead, highlight several opportunities and challenges to be used in clinical practice for managing acute l...
Biosaude, May 17, 2012
... Karen Brajão de Oliveira1, Thiago Cezar Fujita2 , Julie Massayo Maeda Oda2 , Mateus Nobrega A... more ... Karen Brajão de Oliveira1, Thiago Cezar Fujita2 , Julie Massayo Maeda Oda2 , Mateus Nobrega Aoki3, Roberto Iemitsu Tatakihara3, Juliana Laino do Val Carneiro3, Maria Angelica ... 8. Clark-Lewis I, Kim KS, Rajarathnam K, Gong JH, Dewald B, Moser B, Baggiolini M, Sykes BD ...
Rev Bras Anal Clin, 2011
Resumo: No Brasil, o câncer de mama é o que mais causa mortes entre as mulheres com um risco de 5... more Resumo: No Brasil, o câncer de mama é o que mais causa mortes entre as mulheres com um risco de 51 casos a cada 100 mil mulheres. A metástase, principal causa de morte pelo câncer de mama, e dirigida a urna variedade de órgaos vitais, como ossos, pulmões, ...
Current Immunology Reviews, 2015
Revista de Patologia Tropical, 2015
International Journal of Cancer, 2014
Chemokines and its receptors have significant impact on physiological and pathological processes ... more Chemokines and its receptors have significant impact on physiological and pathological processes and studies concerning their association with tumor biology are subject of great interest in scientific community. CXCL12/CXCR4 axis has been widely studied due to its significant role in tumor microenvironment, but it is also important to development and maintenance of tissues and organs, for example, in the brain and cerebellum. Studies have demonstrated that CXCL12 and CXCR4 are required for normal cerebellar development and that dysfunction in this pathway may be involved with medulloblastoma pathogenesis. In this context, a new molecular subgroup has been suggested based on the importance of the association between CXCR4 overexpression and sonic hedgehog subgroup. Treatment using CXCR4 antagonists showed significant results, evidencing the important role and possible therapeutic capacity of CXCR4 in MB. This review summarizes studies on MB cell biology, focusing on a chemokine-receptor axis, CXCL12/CXCR4, that may have implications for treatment strategies once it can improve life expectancy and reduce neurocognitive sequelae of patients with this neoplasia.
Semina: Ciências Biológicas e da Saúde, 2013
sumarios.org
... Karen Brajão de Oliveira1, Thiago Cezar Fujita2 , Julie Massayo Maeda Oda2 , Mateus Nobrega A... more ... Karen Brajão de Oliveira1, Thiago Cezar Fujita2 , Julie Massayo Maeda Oda2 , Mateus Nobrega Aoki3, Roberto Iemitsu Tatakihara3, Juliana Laino do Val Carneiro3, Maria Angelica ... 8. Clark-Lewis I, Kim KS, Rajarathnam K, Gong JH, Dewald B, Moser B, Baggiolini M, Sykes BD ...
Brazilian Archives of Biology and Technology, 2012
Acta Scientiarum. Health Science, 2010
Journal of Clinical Laboratory Analysis
Chemokines and their receptors regulate the trafficking of immune cells during their development,... more Chemokines and their receptors regulate the trafficking of immune cells during their development, inflammation, and tissue repair. The single-nucleotide polymorphism (SNP) rs1801157 (previously known as CXCL12-A/ stromal cell-derived factor-1 (SDF1)-3'A) in CXCL12/SDF1 gene was assessed in breast cancer, Hodgkin's lymphoma (HL), and non-Hodgkin's lymphoma (NHL), since the chemokine CXCL12, previously known as SDF1, and its receptor CXCR4 regulate leukocyte trafficking and many essential biological processes, including tumor growth, angiogenesis, and metastasis of different types of tumors. Genotyping was performed by PCR-RFLP (polymerase chain reaction followed by restriction fragment length polymorphism) using a restriction enzyme HpaII cleavage. No significant difference was observed in genotype distribution between breast cancer patients (GG: 57.3%; GA: 39.8%; AA: 2.9%) and healthy female controls (GG: 62.9%; GA: 33%; AA: 4.1%) nor between HL patients (GG: 61.1%; GA:2...
In the present study, nasal mucus from patients with leprosy were analyzed by PCR using specific ... more In the present study, nasal mucus from patients with leprosy were analyzed by PCR using specific primers for Lsr2 gene of Mycobacterium leprae.
Anticancer research, 2016
The WT1 gene encodes a transcription factor involved in regulation of many cellular processes, in... more The WT1 gene encodes a transcription factor involved in regulation of many cellular processes, including proliferation, differentiation, mRNA processing and apoptosis, besides acting as a transcription repressor of growth factors and their receptors' genes. This gene is expressed at high levels in several types of cancers, including acute leukemias. In this regard, many studies have identified WT1 protein as a tumor antigen, considered a target molecule for clinical application in human acute leukemias. Immunotherapy using WT1 antigen has been effective in stimulating immune responses against leukemic cells. Regarding adoptive immunotherapy, the use of dendritic cells (DCs) for the WT1-specific cytotoxic T cells generation proved to be efficient in the development and maintenance of immunologic cells. Therefore, these therapeutic methods, that provided enthusiasm for moving ahead, highlight several opportunities and challenges to be used in clinical practice for managing acute l...
Este trabalho teve o objetivo de analisar os casos de neoplasias atendidos no Hospital de Câncer ... more Este trabalho teve o objetivo de analisar os casos de neoplasias atendidos no Hospital de Câncer de Londrina (HCL) no ano de 2005, classificando-os de acordo com a Lista de Neoplasias Relacionadas ao Trabalho (PORTARIA 1339/99) para verificar a frequencia de neoplasias que constam da referida lista. Foi realizado um estudo transversal de casos de pacientes residentes em municipios pertencentes a 17a Regional de Saude do Parana, inscritos no Hospital do Câncer de Londrina (HCL). Os dados foram coletados por fonte secundaria, por meio do Registro Hospitalar de Câncer disponivel na instituicao em estudo. Foram selecionados os casos de pacientes conforme dados acerca do tipo de câncer e ocupacao. Dos 784 casos inscritos no servico, 296 casos faziam parte da Lista de Neoplasias Relacionadas ao Trabalho. Os tres tipos de câncer mais frequentes que constavam da lista, foram as neoplasias malignas de pele naomelanoma, seguidas das neoplasias de estomago, bronquios e pulmao. As ocupacoes for...
Chronic myeloid leukemia (CML) is a malignant myeloproliferative disorder that originates from a ... more Chronic myeloid leukemia (CML) is a malignant myeloproliferative disorder that originates from a pluripotent stem cell characterized by abnormal release of the expanded, malignant stem cell clone from the bone marrow into the bloodstream. The vast majority of patients with CML present Bcr-Abl transcripts. Lactate dehydrogenase (LDH) is considered a biochemical marker common for tumor growth, anaerobic glycolysis and has been considered a poor prognostic factor for acute myeloid leukemia. Therefore, this study aimed to evaluate the concentration of LDH in plasma and the detection of the Bcr-Abl transcripts in patients with CML and healthy donors. We analyzed 22 patients demonstrably diagnosed with CML and 56 healthy donors. LDH concentration in plasma was higher in patients with CML. All patients with CML in this study were under treatment, but even so four patients had the Bcr-Abl (b3a2) transcript in peripheral blood. Two out of the four patients with b3a2 showed higher LDH (486 U ...
Molecular Biology Reports
Acute lymphoid leukemia (ALL) is a type of hematological neoplasm that affects the precursor cell... more Acute lymphoid leukemia (ALL) is a type of hematological neoplasm that affects the precursor cells of strains B, T and NK, with a higher incidence in the pediatric range. The pathophysiology of ALL is characterized by chromosomal abnormalities and genetic alterations involved in the differentiation and proliferation of lymphoid precursor cells. Despite the lack of information in the literature, it is believed that leukemogenesis originates from a complex interaction between environmental and genetic factors, which combined lead to cellular modifications. Environmental factors have been evaluated as possible predisposing factors in the development of ALL but there are still conflicting results in the world literature. In this context, the aim of the present review is to discuss the major exogenous factors regarding ALL.
International Journal of Cancer Therapy and Oncology
Anticancer research, 2016
The WT1 gene encodes a transcription factor involved in regulation of many cellular processes, in... more The WT1 gene encodes a transcription factor involved in regulation of many cellular processes, including proliferation, differentiation, mRNA processing and apoptosis, besides acting as a transcription repressor of growth factors and their receptors' genes. This gene is expressed at high levels in several types of cancers, including acute leukemias. In this regard, many studies have identified WT1 protein as a tumor antigen, considered a target molecule for clinical application in human acute leukemias. Immunotherapy using WT1 antigen has been effective in stimulating immune responses against leukemic cells. Regarding adoptive immunotherapy, the use of dendritic cells (DCs) for the WT1-specific cytotoxic T cells generation proved to be efficient in the development and maintenance of immunologic cells. Therefore, these therapeutic methods, that provided enthusiasm for moving ahead, highlight several opportunities and challenges to be used in clinical practice for managing acute l...
Biosaude, May 17, 2012
... Karen Brajão de Oliveira1, Thiago Cezar Fujita2 , Julie Massayo Maeda Oda2 , Mateus Nobrega A... more ... Karen Brajão de Oliveira1, Thiago Cezar Fujita2 , Julie Massayo Maeda Oda2 , Mateus Nobrega Aoki3, Roberto Iemitsu Tatakihara3, Juliana Laino do Val Carneiro3, Maria Angelica ... 8. Clark-Lewis I, Kim KS, Rajarathnam K, Gong JH, Dewald B, Moser B, Baggiolini M, Sykes BD ...
Rev Bras Anal Clin, 2011
Resumo: No Brasil, o câncer de mama é o que mais causa mortes entre as mulheres com um risco de 5... more Resumo: No Brasil, o câncer de mama é o que mais causa mortes entre as mulheres com um risco de 51 casos a cada 100 mil mulheres. A metástase, principal causa de morte pelo câncer de mama, e dirigida a urna variedade de órgaos vitais, como ossos, pulmões, ...
Current Immunology Reviews, 2015
Revista de Patologia Tropical, 2015
International Journal of Cancer, 2014
Chemokines and its receptors have significant impact on physiological and pathological processes ... more Chemokines and its receptors have significant impact on physiological and pathological processes and studies concerning their association with tumor biology are subject of great interest in scientific community. CXCL12/CXCR4 axis has been widely studied due to its significant role in tumor microenvironment, but it is also important to development and maintenance of tissues and organs, for example, in the brain and cerebellum. Studies have demonstrated that CXCL12 and CXCR4 are required for normal cerebellar development and that dysfunction in this pathway may be involved with medulloblastoma pathogenesis. In this context, a new molecular subgroup has been suggested based on the importance of the association between CXCR4 overexpression and sonic hedgehog subgroup. Treatment using CXCR4 antagonists showed significant results, evidencing the important role and possible therapeutic capacity of CXCR4 in MB. This review summarizes studies on MB cell biology, focusing on a chemokine-receptor axis, CXCL12/CXCR4, that may have implications for treatment strategies once it can improve life expectancy and reduce neurocognitive sequelae of patients with this neoplasia.
Semina: Ciências Biológicas e da Saúde, 2013
sumarios.org
... Karen Brajão de Oliveira1, Thiago Cezar Fujita2 , Julie Massayo Maeda Oda2 , Mateus Nobrega A... more ... Karen Brajão de Oliveira1, Thiago Cezar Fujita2 , Julie Massayo Maeda Oda2 , Mateus Nobrega Aoki3, Roberto Iemitsu Tatakihara3, Juliana Laino do Val Carneiro3, Maria Angelica ... 8. Clark-Lewis I, Kim KS, Rajarathnam K, Gong JH, Dewald B, Moser B, Baggiolini M, Sykes BD ...
Brazilian Archives of Biology and Technology, 2012
Acta Scientiarum. Health Science, 2010
Journal of Clinical Laboratory Analysis
Chemokines and their receptors regulate the trafficking of immune cells during their development,... more Chemokines and their receptors regulate the trafficking of immune cells during their development, inflammation, and tissue repair. The single-nucleotide polymorphism (SNP) rs1801157 (previously known as CXCL12-A/ stromal cell-derived factor-1 (SDF1)-3'A) in CXCL12/SDF1 gene was assessed in breast cancer, Hodgkin's lymphoma (HL), and non-Hodgkin's lymphoma (NHL), since the chemokine CXCL12, previously known as SDF1, and its receptor CXCR4 regulate leukocyte trafficking and many essential biological processes, including tumor growth, angiogenesis, and metastasis of different types of tumors. Genotyping was performed by PCR-RFLP (polymerase chain reaction followed by restriction fragment length polymorphism) using a restriction enzyme HpaII cleavage. No significant difference was observed in genotype distribution between breast cancer patients (GG: 57.3%; GA: 39.8%; AA: 2.9%) and healthy female controls (GG: 62.9%; GA: 33%; AA: 4.1%) nor between HL patients (GG: 61.1%; GA:2...