Thierry Ancelle - Academia.edu (original) (raw)

Papers by Thierry Ancelle

The American journal of tropical medicine and hygiene, 1998

An outbreak of 538 cases of trichinellosis occurred in France in December 1993. Seven cases devel... more An outbreak of 538 cases of trichinellosis occurred in France in December 1993. Seven cases developed neurotrichinosis and 23 had cardiologic complications. No deaths were recorded. Two patients had a positive muscle biopsy showing living Trichinella larvae. One of them was typed as Trichinella spiralis. A case-control study showed that horse meat was the only meat associated with illness (odds ratio = 80.7). The risk of illness increased with the amount of horse meat eaten and when it was consumed raw. The cases, which were spread out in five foci, bought horse meat from five butchers who had received parts of a single horse carcass imported in November 1993 from Canada. The Trichinella International Screening Program, implemented since 1985 after two similar episodes involving a thousand cases, failed to detect the incriminated horse carcass. This new horse meat-related outbreak led to modifications of the internationally recommended screening methods whereby the weight of meat sa...

European Journal of Clinical Microbiology & Infectious Diseases, 1997

An outbreak of nosocomial diarrhea that occurred in a pediatric orthopedic service between 1 Dece... more An outbreak of nosocomial diarrhea that occurred in a pediatric orthopedic service between 1 December 1993 and 15 April 1994 is reported. A total of 37 patients (mean age, 9.6 years; range, 2 months-19.3 years) were involved in the outbreak, including six patients with bacteriologically documentedClostridium difficile infection. A multivariate analysis identified lincomycin treatment for at least three days as

Journal of clinical microbiology, 1999

We reviewed the files of 110 women with Toxoplasma seroconversion during pregnancy. Prenatal diag... more We reviewed the files of 110 women with Toxoplasma seroconversion during pregnancy. Prenatal diagnosis was attempted for 94 women by amniotic fluid sampling. Toxoplasma gondii was detected by PCR, with or without tissue culture and mouse inoculation. The early neonatal diagnostic procedure included placental testing by PCR and/or mouse inoculation, cord blood serological testing, and comparison of maternal and newborn antibodies by Western blotting (WB). Serological follow-up of the infants was conducted during the first year of life or until the diagnosis of congenital toxoplasmosis (CT) could be ruled out. Congenital infection was diagnosed in 27 individuals (20 live births) in the prenatal and/or neonatal period. The sensitivity and specificity of prenatal diagnosis were 81 and 100%, respectively. Placental examination was positive for 66.7% of individuals with CT and was always negative for neonates without CT. Cord blood serology detected immunoglobulin M (IgM) and/or IgA in 80...

Parasitology Research, 1991

... Jean Dupouy-Camet 1, Claude Soul~ 1, Jean-Pierre Guillou 2, Evelyne Rouer 3, Sandra Lavareda ... more ... Jean Dupouy-Camet 1, Claude Soul~ 1, Jean-Pierre Guillou 2, Evelyne Rouer 3, Sandra Lavareda de Souza 3, Thierry Ancelle 1 ... by electrophore-sis in 3% agarose gels and then transferred to Hybond-N nylon membranes (Amersham) according to the manu-facturer's protocol ...

Revue d'Épidémiologie et de Santé Publique, 2009

Background. -A program of systematic serology screening for toxoplasmosis during pregnancy has be... more Background. -A program of systematic serology screening for toxoplasmosis during pregnancy has been running in France since 1978. The program involves monthly follow-ups for all non-immune pregnant women. Due to the steady decline in the seroprevalence of toxoplasmosis, the cost of the program is steadily increasing. Current screening is based on the detection of IgG and IgM isotypes. The aim of this work was to estimate the benefit of replacing combined dosage of two isotypes, by an alternative strategy that detects total anti-Toxoplasma immunoglobulins.

Veterinary Parasitology, 1989

Soul~, C., Dupouy-Camet, J., Georges, P., Ancelle, T., Gillet, J.P., Vaissaire, J., Delvigne, A. ... more Soul~, C., Dupouy-Camet, J., Georges, P., Ancelle, T., Gillet, J.P., Vaissaire, J., Delvigne, A. and Plateau, E., 1989. Experimental trichinellosis in horses: biological and parasitological evaluation. Vet. Parasitol., 31: 19-36.

Journal of Clinical Microbiology, 2009

Facteurs de survenue d'une toxoplasmose oculaire Les toxoplasmoses acquises ou congénitales sont ... more Facteurs de survenue d'une toxoplasmose oculaire Les toxoplasmoses acquises ou congénitales sont fréquemment compliquées par des lésions oculaires. Le diagnostic de ces complications se fonde sur les aspects cliniques, la réponse au traitement spécifique et les résultats des analyses biologiques. L'incidence et la prévalence de ces complications sont difficiles à établir avec précision et dépendent de la prévalence de la parasitose dans la population générale et sont affectées par le type d'exposition au parasite, par la génétique de l'hôte et du parasite et par la réponse immune induite chez l'hôte par le parasite.

La Revue de médecine …, 2008

Propos. -La vaccination, en prévenant les infections, présente un intérêt certain pour les sujets... more Propos. -La vaccination, en prévenant les infections, présente un intérêt certain pour les sujets immunodéprimés. L'objectif de cet article est de faire un état des lieux des données concernant l'efficacité (en termes d'immunogénicité) et la tolérance des vaccins viraux disponibles en France et de synthétiser les recommandations existantes pour quatre grands groupes de patients : transplantés d'organes solides, greffés de moelle, infectés par le VIH et traités par immunosuppresseurs pour une maladie systémique.

European Journal of Clinical Microbiology & Infectious Diseases, 1997

European Journal of Clinical Microbiology & Infectious Diseases, 2007

In the study presented here, PCR, microscopic examination and culture of corneal samples were com... more In the study presented here, PCR, microscopic examination and culture of corneal samples were compared as methods of confirming the clinical diagnosis of Acanthamoeba keratitis, a serious ocular infection that is difficult to diagnose and threatens eyesight. The three methods were applied to isolates obtained from 513 patients with clinical signs or risk factors suggesting Acanthamoeba infection. Acanthamoeba keratitis was diagnosed in 12 of these patients. Combined PCR assays were more sensitive (94%) than either microscopic examination (33%) or culture (7%). The Acanthamoeba isolates were characterized using DNA sequence analysis of the nuclear small-subunit rRNA gene, and T4 was the predominant genotype found.

Emerging Infectious Diseases, 2011

Little is known about severe imported Plasmodium falciparum malaria in industrialized countries w... more Little is known about severe imported Plasmodium falciparum malaria in industrialized countries where the disease is not endemic because most studies have been case reports or have included <200 patients. To identify factors independently associated with the severity of P. falciparum, we conducted a retrospective study using surveillance data obtained from 21,888 P. falciparum patients in France during 1996-2003; 832 were classified as having severe malaria. The global case-fatality rate was 0.4% and the rate of severe malaria was ≈3.8%. Factors independently associated with severe imported P. falciparum malaria were older age, European origin, travel to eastern Africa, absence of chemoprophylaxis, initial visit to a general practitioner, time to diagnosis of 4 to 12 days, and diagnosis during the fall-winter season. Pretravel advice should take into account these factors and promote the use of antimalarial chemoprophylaxis for every traveler, with a particular focus on nonimmune travelers and elderly persons.

Arthritis Care & Research, 2013

Methods. All adult travelers on systemic corticosteroid therapy who had received the YF17D vaccin... more Methods. All adult travelers on systemic corticosteroid therapy who had received the YF17D vaccine in 24 French vaccination centers were prospectively enrolled and matched with healthy controls (1:2) on age and history of YF17D immunization. Safety was assessed in a self-administered standardized questionnaire within 10 days after immunization. YF-specific neutralizing antibody titers were measured 6 months after vaccination in patients receiving corticosteroids. Results. Between July 2008 and February 2011, 102 vaccine recipients completed the safety study (34 receiving corticosteroids and 68 controls). The median age was 54.9 years (interquartile range [IQR] 45.1-60.3 years) and 45 participants had a history of previous YF17D immunization. The median time receiving corticosteroid therapy was 10 months (IQR 1-67 months) and the prednisone or equivalent dosage was 7 mg/day (IQR 5-20). Main indications were autoimmune diseases (n ‫؍‬ 14), rheumatoid arthritis (n ‫؍‬ 9), and upper respiratory tract infections (n ‫؍‬ 8). No serious adverse event was reported; however, patients receiving corticosteroids reported more frequent moderate/severe local reactions than controls (12% and 2%, respectively; relative risk 8.0, 95% confidence interval 1.4 -45.9). All subjects receiving corticosteroids who were tested (n ‫؍‬ 20) had neutralizing antibody titers >10 after vaccination. Conclusion. After YF17D immunization, moderate/severe local reactions may be more frequent in patients receiving systemic corticosteroid therapy. Immunogenicity seems satisfactory. Large-scale studies are needed to confirm these results.

Journal of Clinical Microbiology, 2009

Over the past few years, a number of new nucleic acid extraction methods and extraction platforms... more Over the past few years, a number of new nucleic acid extraction methods and extraction platforms using chemistry combined with magnetic or silica particles have been developed, in combination with instruments to facilitate the extraction procedure. The objective of the present study was to investigate the suitability of these automated methods for the isolation of Toxoplasma gondii DNA from amniotic fluid (AF). Therefore, three automated procedures were compared to two commercialized manual extraction methods. The MagNA Pure Compact (Roche), BioRobot EZ1 (Qiagen), and easyMAG (bioMérieux) automated procedures were compared to two manual DNA extraction kits, the QIAamp DNA minikit (Qiagen) and the High Pure PCR template preparation kit (Roche). Evaluation was carried out with two specific Toxoplasma PCRs (targeting the 529-bp repeat element), inhibitor search PCRs, and human beta-globin PCRs. The samples each consisted of 4 ml of AF with or without a calibrated Toxoplasma gondii RH strain suspension (0, 1, 2.5, 5, and 25 tachyzoites/ml). All PCR assays were laboratory-developed real-time PCR assays, using either TaqMan or fluorescent resonance energy transfer probes. A total of 1,178 PCRs were performed, including 978 Toxoplasma PCRs. The automated and manual methods were similar in sensitivity for DNA extraction from T. gondii at the highest concentration (25 Toxoplasma gondii cells/ml). However, our results showed that the DNA extraction procedures led to variable efficacy in isolating low concentrations of tachyzoites in AF samples (<5 Toxoplasma gondii cells/ml), a difference that might have repercussions since low parasite concentrations in AF exist and can lead to congenital toxoplasmosis.

The American journal of tropical medicine and hygiene, 1998

An outbreak of 538 cases of trichinellosis occurred in France in December 1993. Seven cases devel... more An outbreak of 538 cases of trichinellosis occurred in France in December 1993. Seven cases developed neurotrichinosis and 23 had cardiologic complications. No deaths were recorded. Two patients had a positive muscle biopsy showing living Trichinella larvae. One of them was typed as Trichinella spiralis. A case-control study showed that horse meat was the only meat associated with illness (odds ratio = 80.7). The risk of illness increased with the amount of horse meat eaten and when it was consumed raw. The cases, which were spread out in five foci, bought horse meat from five butchers who had received parts of a single horse carcass imported in November 1993 from Canada. The Trichinella International Screening Program, implemented since 1985 after two similar episodes involving a thousand cases, failed to detect the incriminated horse carcass. This new horse meat-related outbreak led to modifications of the internationally recommended screening methods whereby the weight of meat sa...

European Journal of Clinical Microbiology & Infectious Diseases, 1997

An outbreak of nosocomial diarrhea that occurred in a pediatric orthopedic service between 1 Dece... more An outbreak of nosocomial diarrhea that occurred in a pediatric orthopedic service between 1 December 1993 and 15 April 1994 is reported. A total of 37 patients (mean age, 9.6 years; range, 2 months-19.3 years) were involved in the outbreak, including six patients with bacteriologically documentedClostridium difficile infection. A multivariate analysis identified lincomycin treatment for at least three days as

Journal of clinical microbiology, 1999

We reviewed the files of 110 women with Toxoplasma seroconversion during pregnancy. Prenatal diag... more We reviewed the files of 110 women with Toxoplasma seroconversion during pregnancy. Prenatal diagnosis was attempted for 94 women by amniotic fluid sampling. Toxoplasma gondii was detected by PCR, with or without tissue culture and mouse inoculation. The early neonatal diagnostic procedure included placental testing by PCR and/or mouse inoculation, cord blood serological testing, and comparison of maternal and newborn antibodies by Western blotting (WB). Serological follow-up of the infants was conducted during the first year of life or until the diagnosis of congenital toxoplasmosis (CT) could be ruled out. Congenital infection was diagnosed in 27 individuals (20 live births) in the prenatal and/or neonatal period. The sensitivity and specificity of prenatal diagnosis were 81 and 100%, respectively. Placental examination was positive for 66.7% of individuals with CT and was always negative for neonates without CT. Cord blood serology detected immunoglobulin M (IgM) and/or IgA in 80...

Parasitology Research, 1991

... Jean Dupouy-Camet 1, Claude Soul~ 1, Jean-Pierre Guillou 2, Evelyne Rouer 3, Sandra Lavareda ... more ... Jean Dupouy-Camet 1, Claude Soul~ 1, Jean-Pierre Guillou 2, Evelyne Rouer 3, Sandra Lavareda de Souza 3, Thierry Ancelle 1 ... by electrophore-sis in 3% agarose gels and then transferred to Hybond-N nylon membranes (Amersham) according to the manu-facturer's protocol ...

Revue d'Épidémiologie et de Santé Publique, 2009

Background. -A program of systematic serology screening for toxoplasmosis during pregnancy has be... more Background. -A program of systematic serology screening for toxoplasmosis during pregnancy has been running in France since 1978. The program involves monthly follow-ups for all non-immune pregnant women. Due to the steady decline in the seroprevalence of toxoplasmosis, the cost of the program is steadily increasing. Current screening is based on the detection of IgG and IgM isotypes. The aim of this work was to estimate the benefit of replacing combined dosage of two isotypes, by an alternative strategy that detects total anti-Toxoplasma immunoglobulins.

Veterinary Parasitology, 1989

Soul~, C., Dupouy-Camet, J., Georges, P., Ancelle, T., Gillet, J.P., Vaissaire, J., Delvigne, A. ... more Soul~, C., Dupouy-Camet, J., Georges, P., Ancelle, T., Gillet, J.P., Vaissaire, J., Delvigne, A. and Plateau, E., 1989. Experimental trichinellosis in horses: biological and parasitological evaluation. Vet. Parasitol., 31: 19-36.

Journal of Clinical Microbiology, 2009

Facteurs de survenue d'une toxoplasmose oculaire Les toxoplasmoses acquises ou congénitales sont ... more Facteurs de survenue d'une toxoplasmose oculaire Les toxoplasmoses acquises ou congénitales sont fréquemment compliquées par des lésions oculaires. Le diagnostic de ces complications se fonde sur les aspects cliniques, la réponse au traitement spécifique et les résultats des analyses biologiques. L'incidence et la prévalence de ces complications sont difficiles à établir avec précision et dépendent de la prévalence de la parasitose dans la population générale et sont affectées par le type d'exposition au parasite, par la génétique de l'hôte et du parasite et par la réponse immune induite chez l'hôte par le parasite.

La Revue de médecine …, 2008

Propos. -La vaccination, en prévenant les infections, présente un intérêt certain pour les sujets... more Propos. -La vaccination, en prévenant les infections, présente un intérêt certain pour les sujets immunodéprimés. L'objectif de cet article est de faire un état des lieux des données concernant l'efficacité (en termes d'immunogénicité) et la tolérance des vaccins viraux disponibles en France et de synthétiser les recommandations existantes pour quatre grands groupes de patients : transplantés d'organes solides, greffés de moelle, infectés par le VIH et traités par immunosuppresseurs pour une maladie systémique.

European Journal of Clinical Microbiology & Infectious Diseases, 1997

European Journal of Clinical Microbiology & Infectious Diseases, 2007

In the study presented here, PCR, microscopic examination and culture of corneal samples were com... more In the study presented here, PCR, microscopic examination and culture of corneal samples were compared as methods of confirming the clinical diagnosis of Acanthamoeba keratitis, a serious ocular infection that is difficult to diagnose and threatens eyesight. The three methods were applied to isolates obtained from 513 patients with clinical signs or risk factors suggesting Acanthamoeba infection. Acanthamoeba keratitis was diagnosed in 12 of these patients. Combined PCR assays were more sensitive (94%) than either microscopic examination (33%) or culture (7%). The Acanthamoeba isolates were characterized using DNA sequence analysis of the nuclear small-subunit rRNA gene, and T4 was the predominant genotype found.

Emerging Infectious Diseases, 2011

Little is known about severe imported Plasmodium falciparum malaria in industrialized countries w... more Little is known about severe imported Plasmodium falciparum malaria in industrialized countries where the disease is not endemic because most studies have been case reports or have included <200 patients. To identify factors independently associated with the severity of P. falciparum, we conducted a retrospective study using surveillance data obtained from 21,888 P. falciparum patients in France during 1996-2003; 832 were classified as having severe malaria. The global case-fatality rate was 0.4% and the rate of severe malaria was ≈3.8%. Factors independently associated with severe imported P. falciparum malaria were older age, European origin, travel to eastern Africa, absence of chemoprophylaxis, initial visit to a general practitioner, time to diagnosis of 4 to 12 days, and diagnosis during the fall-winter season. Pretravel advice should take into account these factors and promote the use of antimalarial chemoprophylaxis for every traveler, with a particular focus on nonimmune travelers and elderly persons.

Arthritis Care & Research, 2013

Methods. All adult travelers on systemic corticosteroid therapy who had received the YF17D vaccin... more Methods. All adult travelers on systemic corticosteroid therapy who had received the YF17D vaccine in 24 French vaccination centers were prospectively enrolled and matched with healthy controls (1:2) on age and history of YF17D immunization. Safety was assessed in a self-administered standardized questionnaire within 10 days after immunization. YF-specific neutralizing antibody titers were measured 6 months after vaccination in patients receiving corticosteroids. Results. Between July 2008 and February 2011, 102 vaccine recipients completed the safety study (34 receiving corticosteroids and 68 controls). The median age was 54.9 years (interquartile range [IQR] 45.1-60.3 years) and 45 participants had a history of previous YF17D immunization. The median time receiving corticosteroid therapy was 10 months (IQR 1-67 months) and the prednisone or equivalent dosage was 7 mg/day (IQR 5-20). Main indications were autoimmune diseases (n ‫؍‬ 14), rheumatoid arthritis (n ‫؍‬ 9), and upper respiratory tract infections (n ‫؍‬ 8). No serious adverse event was reported; however, patients receiving corticosteroids reported more frequent moderate/severe local reactions than controls (12% and 2%, respectively; relative risk 8.0, 95% confidence interval 1.4 -45.9). All subjects receiving corticosteroids who were tested (n ‫؍‬ 20) had neutralizing antibody titers >10 after vaccination. Conclusion. After YF17D immunization, moderate/severe local reactions may be more frequent in patients receiving systemic corticosteroid therapy. Immunogenicity seems satisfactory. Large-scale studies are needed to confirm these results.

Journal of Clinical Microbiology, 2009

Over the past few years, a number of new nucleic acid extraction methods and extraction platforms... more Over the past few years, a number of new nucleic acid extraction methods and extraction platforms using chemistry combined with magnetic or silica particles have been developed, in combination with instruments to facilitate the extraction procedure. The objective of the present study was to investigate the suitability of these automated methods for the isolation of Toxoplasma gondii DNA from amniotic fluid (AF). Therefore, three automated procedures were compared to two commercialized manual extraction methods. The MagNA Pure Compact (Roche), BioRobot EZ1 (Qiagen), and easyMAG (bioMérieux) automated procedures were compared to two manual DNA extraction kits, the QIAamp DNA minikit (Qiagen) and the High Pure PCR template preparation kit (Roche). Evaluation was carried out with two specific Toxoplasma PCRs (targeting the 529-bp repeat element), inhibitor search PCRs, and human beta-globin PCRs. The samples each consisted of 4 ml of AF with or without a calibrated Toxoplasma gondii RH strain suspension (0, 1, 2.5, 5, and 25 tachyzoites/ml). All PCR assays were laboratory-developed real-time PCR assays, using either TaqMan or fluorescent resonance energy transfer probes. A total of 1,178 PCRs were performed, including 978 Toxoplasma PCRs. The automated and manual methods were similar in sensitivity for DNA extraction from T. gondii at the highest concentration (25 Toxoplasma gondii cells/ml). However, our results showed that the DNA extraction procedures led to variable efficacy in isolating low concentrations of tachyzoites in AF samples (<5 Toxoplasma gondii cells/ml), a difference that might have repercussions since low parasite concentrations in AF exist and can lead to congenital toxoplasmosis.