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Papers by Thomas Barnett

Research paper thumbnail of Carcinoembryonic Antigens: Alternative Splicing Accounts for the Multiple mRNAs that Code for Novel Members of the Carcinoembryonic Antigen Family

Journal of Cell Biology, 1989

The recent cloning of complete cDNAs encoding carcinoembryonic antigen (CEA) and nonspecific cros... more The recent cloning of complete cDNAs encoding carcinoembryonic antigen (CEA) and nonspecific cross-reacting antigen has revealed the existence of a new gene family belonging to the immunoglobulin gene superfamily. We have reported the isolation of a partial CEA cDNA and of L-cell transfectant cell lines that express human antigens cross-reactive with commercial antibodies directed to native CEA (

Research paper thumbnail of Carcinoembryonic antigens: alternative splicing accounts for the multiple mRNAs that code for novel members of the carcinoembryonic antigen family

The Journal of cell biology, 1989

Abstract. The recent cloning of complete cDNAs en-coding carcinoembryonic antigen (CEA) and non-s... more Abstract. The recent cloning of complete cDNAs en-coding carcinoembryonic antigen (CEA) and non-specific cross-reacting antigen has revealed the exis-tence of a new gene family belonging to the immunoglobulin gene superfamily. We have reported the isolation of a partial CEA ...

Research paper thumbnail of PEGylation of Factor VIII Reduces the Inhibitory Effects of Human Antibody Inhibitors on the Factor VIII Molecule

Blood

Replacement therapy with plasma-derived or recombinant Factor VIII (FVIII) has successfully reduc... more Replacement therapy with plasma-derived or recombinant Factor VIII (FVIII) has successfully reduced mortality and morbidity and improved the quality of life for patients with hemophilia A. However, up to 30% of patients develop antibody inhibitors to the FVIII molecule. Inhibitors reduce the efficacy of FVIII, increase the cost of treatment, and greatly increase the risk for life-threatening bleeding events in these patients. In the hopes of developing a more efficacious FVIII therapeutic for hemophilia A patients with inhibitors, we initiated studies on the PEGylation of B-region deleted FVIII (FVIII-BDD) with different sizes of polyethylene glycol (PEG) and investigated the inhibitory effects of anti-FVIII inhibitors on the activity of PEGylated FVIII-BDDs. Applying a site-specific mutagenesis technique to the FVIII-BDD molecule, an amino-acid residue at the inhibitor binding site was changed to cysteine. Mutated FVIII-BDD was purified and PEG molecules of different sizes were add...

Research paper thumbnail of The isolation and characterization of Drosophila yolk protein genes

Research paper thumbnail of Immunoassay for NCA-BT in blood samples of breast cancer patients

Research paper thumbnail of A cDNA coding for carcinoembryonic antigen

Research paper thumbnail of von Willebrand factor (vWF)-containing preparations, and methods, kits, and uses related thereto

Research paper thumbnail of Continuation - Site Directed Modification of Fviii

Research paper thumbnail of CDNA coding for carcinoembryonic antigen

Research paper thumbnail of Site-directed modification of FVIII

Research paper thumbnail of Characterization of two new members of the pregnancy-specific .beta.1-glycoprotein family from the myeloid cell line KG-1 and suggestion of two distinct classes of transcription unit

Biochemistry Usa, Jun 11, 1990

Pregnancy-specific &glycoproteins (PSGs) represent a large group (-12-1 5) of proteins, related t... more Pregnancy-specific &glycoproteins (PSGs) represent a large group (-12-1 5) of proteins, related to members of the carcinoembryonic antigen family, that are abundant in placental tissue and in the sera of pregnant women. We describe the isolation and characterization of two additional PSG cDNAs, PSG9 and PSG10, whose transcripts are largely expressed in placental tissue and to a lesser extent in some other cell types, including myeloid cells differentiated to granulocytes. PSG9 and PSGlO are representatives of I Abbreviations: PSG, pregnancy-specific &-glycoprotein; CEA, carcinoembryonic antigen; NCA, nonspecific cross-reacting antigen; BGP I, biliary glycoprotein I; UTR, untranslated region; PMA, phorbol 12myristate 13-acetate; aa, amino acid.

Research paper thumbnail of Antibody preparations specifically binding to unique determinants of CEA antigens or fragments thereof and use of the antibody preparations in immunoassays

Research paper thumbnail of Factor VIII Conjugates

Research paper thumbnail of Translocation Signal Facilitated Nuclear Delivery of Macromolecules

Research paper thumbnail of Site-directed modification of FVIII

Research paper thumbnail of Targeted Delivery of Factor VIII Proteins to Platelets

Research paper thumbnail of CDNAS coding for members of the carcinoembryonic antigen family

Research paper thumbnail of cDNA coding for carcinoembryonic antigen (CEA)

Research paper thumbnail of Proposed Nomenclature for the Carcinoembryonic Antigen (CEA) Gene Family

Research paper thumbnail of Large scale production of DNA probes

A process for the production of a single strand of a nucleic acid is described comprising: (a) ta... more A process for the production of a single strand of a nucleic acid is described comprising: (a) tailing a 3' end of a nucleic acid with a terminal deoxynucleotidyl transferase and a ribonucleoside triphosphate, (b) oxidizing the product of step (a) to produce a nucleic acid with a pendant aldehyde group, (c) reacting the product the step (b) with a solid support, the solid support containing one or more NHâ groups, (d) reacting the rpduct of step (c) with a primer nucleic acid to form a hydrid, (e) extending the primer on the hybrid with DNA polymerase and a nucleoside triphosphate, (f) denaturing the product of step (e), and (g) separating the product of step (f) into a soluble product and an insoluble product.

Research paper thumbnail of Carcinoembryonic Antigens: Alternative Splicing Accounts for the Multiple mRNAs that Code for Novel Members of the Carcinoembryonic Antigen Family

Journal of Cell Biology, 1989

The recent cloning of complete cDNAs encoding carcinoembryonic antigen (CEA) and nonspecific cros... more The recent cloning of complete cDNAs encoding carcinoembryonic antigen (CEA) and nonspecific cross-reacting antigen has revealed the existence of a new gene family belonging to the immunoglobulin gene superfamily. We have reported the isolation of a partial CEA cDNA and of L-cell transfectant cell lines that express human antigens cross-reactive with commercial antibodies directed to native CEA (

Research paper thumbnail of Carcinoembryonic antigens: alternative splicing accounts for the multiple mRNAs that code for novel members of the carcinoembryonic antigen family

The Journal of cell biology, 1989

Abstract. The recent cloning of complete cDNAs en-coding carcinoembryonic antigen (CEA) and non-s... more Abstract. The recent cloning of complete cDNAs en-coding carcinoembryonic antigen (CEA) and non-specific cross-reacting antigen has revealed the exis-tence of a new gene family belonging to the immunoglobulin gene superfamily. We have reported the isolation of a partial CEA ...

Research paper thumbnail of PEGylation of Factor VIII Reduces the Inhibitory Effects of Human Antibody Inhibitors on the Factor VIII Molecule

Blood

Replacement therapy with plasma-derived or recombinant Factor VIII (FVIII) has successfully reduc... more Replacement therapy with plasma-derived or recombinant Factor VIII (FVIII) has successfully reduced mortality and morbidity and improved the quality of life for patients with hemophilia A. However, up to 30% of patients develop antibody inhibitors to the FVIII molecule. Inhibitors reduce the efficacy of FVIII, increase the cost of treatment, and greatly increase the risk for life-threatening bleeding events in these patients. In the hopes of developing a more efficacious FVIII therapeutic for hemophilia A patients with inhibitors, we initiated studies on the PEGylation of B-region deleted FVIII (FVIII-BDD) with different sizes of polyethylene glycol (PEG) and investigated the inhibitory effects of anti-FVIII inhibitors on the activity of PEGylated FVIII-BDDs. Applying a site-specific mutagenesis technique to the FVIII-BDD molecule, an amino-acid residue at the inhibitor binding site was changed to cysteine. Mutated FVIII-BDD was purified and PEG molecules of different sizes were add...

Research paper thumbnail of The isolation and characterization of Drosophila yolk protein genes

Research paper thumbnail of Immunoassay for NCA-BT in blood samples of breast cancer patients

Research paper thumbnail of A cDNA coding for carcinoembryonic antigen

Research paper thumbnail of von Willebrand factor (vWF)-containing preparations, and methods, kits, and uses related thereto

Research paper thumbnail of Continuation - Site Directed Modification of Fviii

Research paper thumbnail of CDNA coding for carcinoembryonic antigen

Research paper thumbnail of Site-directed modification of FVIII

Research paper thumbnail of Characterization of two new members of the pregnancy-specific .beta.1-glycoprotein family from the myeloid cell line KG-1 and suggestion of two distinct classes of transcription unit

Biochemistry Usa, Jun 11, 1990

Pregnancy-specific &glycoproteins (PSGs) represent a large group (-12-1 5) of proteins, related t... more Pregnancy-specific &glycoproteins (PSGs) represent a large group (-12-1 5) of proteins, related to members of the carcinoembryonic antigen family, that are abundant in placental tissue and in the sera of pregnant women. We describe the isolation and characterization of two additional PSG cDNAs, PSG9 and PSG10, whose transcripts are largely expressed in placental tissue and to a lesser extent in some other cell types, including myeloid cells differentiated to granulocytes. PSG9 and PSGlO are representatives of I Abbreviations: PSG, pregnancy-specific &-glycoprotein; CEA, carcinoembryonic antigen; NCA, nonspecific cross-reacting antigen; BGP I, biliary glycoprotein I; UTR, untranslated region; PMA, phorbol 12myristate 13-acetate; aa, amino acid.

Research paper thumbnail of Antibody preparations specifically binding to unique determinants of CEA antigens or fragments thereof and use of the antibody preparations in immunoassays

Research paper thumbnail of Factor VIII Conjugates

Research paper thumbnail of Translocation Signal Facilitated Nuclear Delivery of Macromolecules

Research paper thumbnail of Site-directed modification of FVIII

Research paper thumbnail of Targeted Delivery of Factor VIII Proteins to Platelets

Research paper thumbnail of CDNAS coding for members of the carcinoembryonic antigen family

Research paper thumbnail of cDNA coding for carcinoembryonic antigen (CEA)

Research paper thumbnail of Proposed Nomenclature for the Carcinoembryonic Antigen (CEA) Gene Family

Research paper thumbnail of Large scale production of DNA probes

A process for the production of a single strand of a nucleic acid is described comprising: (a) ta... more A process for the production of a single strand of a nucleic acid is described comprising: (a) tailing a 3' end of a nucleic acid with a terminal deoxynucleotidyl transferase and a ribonucleoside triphosphate, (b) oxidizing the product of step (a) to produce a nucleic acid with a pendant aldehyde group, (c) reacting the product the step (b) with a solid support, the solid support containing one or more NHâ groups, (d) reacting the rpduct of step (c) with a primer nucleic acid to form a hydrid, (e) extending the primer on the hybrid with DNA polymerase and a nucleoside triphosphate, (f) denaturing the product of step (e), and (g) separating the product of step (f) into a soluble product and an insoluble product.

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