Thomas Terry - Academia.edu (original) (raw)

Papers by Thomas Terry

Journal of College Science Teaching, 1993

Journal of College Science Teaching, 1980

Proceedings of the National Academy of Sciences, 1975

Membrane fractions from chick embryo cells manifesting viral interference mediated by interferon ... more Membrane fractions from chick embryo cells manifesting viral interference mediated by interferon or poly(I)-poly(C) contain high levels of an alkaline ribonuclease. Enhanced RNase activity is not observed when inhibitors of cell protein or RNA synthesis are present during interferon treatment, or when heterologous interferon is used. The RNase associated with comparable membrane fractions from cells treated with mock-interferon is about 1/10 as active, and shows qualitative differences. In principle, divergent views of interferon action may be reconciled to a common mode of action by postulating that viral interference results from a newly induced or activated RNase of cellular origin and proper specificity that acts to reduce the accumulation and functional capacity of newly synthesized viral RNAs, particularly mRNA. Previous data in support of interferon's acting to inhibit virion-derived transcription in vivo are now interpreted as demonstrating enhanced degradation of viral ...

Journal of Ultrastructure Research, 1972

... In addi-tion, it should be possible to morphologically identify membrane regions rich in unsa... more ... In addi-tion, it should be possible to morphologically identify membrane regions rich in unsaturated fatty acids in any membrane which contains specialized regions of this type. ... 15.RACKER, E., HORSTMAN, LL, KLING, D. and FESSENDEN-RADEN, JM, J. Biol. Chem. ...

BioScience, 1999

Weaving the Web into biology teaching The World Wide Web has rapidly become part of the education... more Weaving the Web into biology teaching The World Wide Web has rapidly become part of the educational landscape. Students typically love it: it is new, it is fun to use, it is graphically and interactively rich, and it affords access to many types of information, academic and otherwise. Most of today's students arrive at college already having some experience with the Web, and some are adept at Web page design and construction. Many students turn to the Web rather than the library to search for information. On nearly every college campus, educators are incorporating one or more Web-based uses into their courses. Yet for several reasons, the Web has not yet become an integral part of the teaching process. Among these reasons is that Web technology is too new, and too rapidly evolving, for most biologists to have had time to explore possible teaching applications. The time required to develop useful Web applications and procedures has proven a substantial barrier to many faculty members already stretched thin with time commitments. In addition, potentially useful educational material available on the Web, although abundant, is disorganized, generally shortlived, and often difficult to authenticate. Finally, it has taken some time for teachers who are "early adopters" of the Web to explore the landscape of possibilities and develop interesting models of possible applications. Many educators are still waiting to be convinced that the Web offers ways of significantly improving the teaching process beyond what they currently do. Fortunately, most of these barriers to using the Web in teaching are diminishing or disappearing. Most academic institutions now have a substantial and growing investment in Internet-linked computers, for both faculty and student use. Many off-campus students have computers and Internet connections. The time and energy required to develop Web materials is considerably lessened by new software packages that provide tools for creating extensive Web sites. Biologically interesting teaching ideas and applications available on the Web are multiplying rapidly, and pioneering educators have demonstrated a number of pedagogically useful applications of the Web. My goal in this article is twofold: to discuss current ways of using and producing documents on the Web, and to survey a variety of uses of the Web for teaching in the biological sciences. All citations from this article are listed on a Web page where citations are hypertext links (Terry 1999a).

Biochimica et Biophysica Acta (BBA) - Biomembranes, 1972

and on dissolution in 1% sodium dodecyl sulphate, spectra analysis indicated the presence of the ... more and on dissolution in 1% sodium dodecyl sulphate, spectra analysis indicated the presence of the membrane cytochromes. The organic solvent and aqueous phases (i.e. I and 3) were, of course, invariably present, but Phases 2 and 4 were variable. The solvent phase up to, but not including, the interfacial material was carefully removed with a chilled Pasteur pipette. The aqueous phase was adjusted to 3 ml with distilled water and two further extractions performed. After a third extraction, a Pasteur pipette was introduced beneath the interface and the aqueous phase removed, taking care not to disturb the floating, interfacial layer or the pellet. The aqueous phase was dialyzed at o °C against several changes of buffer, its final volume measured and samples taken for protein, asp radioactivity and enzymatic determinations. It was imperative to keep the temperature well controlled during extraction, as marked variability in results was observed if temperature fluctuations were allowed to occur. Radioactivity measurements Organic solvent phase extracts were each dried under a stream of N 2 gas and taken up into 3 ml of chloroform. Samples of o.o5-o.1 ml were applied to ~rhatman glass fiber paper discs wb.ich were dried and placed on planchets and the 3~p determined in a Nuclear Chicago gas-flow detector. Radioactivity in the aqueous phases was similarly determined, Total phospholipid radioactivity was determined by extracting 3 ml of an aqueous suspension of membranes with chloroform-methanol (I :2, v/v) by the method of Bligh and Dyer 11. Two extractions were performed, the two organic phases combined and dried under a stream of N 2 gas. The lipid was dissolved in 3 ml of chloroform and kept at-20 °C until required. Great difficulty was encountered in attempting to disperse the pellets and interfacial layers. The residual 3zp remaining in these combined phases was therefore calculated by substracting the 32p in the aqueous and solvent phases from the total as determined by the chloroformmethanol extractions. Chromatography Paper chromatography of the lipid extracts was performed on silica-gel loaded paper (Whatman no. SG-8I), using the solvent system of Wuthier lz (chloroformmethanol-diisobutyl ketone-acetic acid-water (45:15:20:30:4, by vol.)). Lipids were located by staining with Rhodamine 6G (o.oo12 % w/v) in distilled water, the spots cut out, placed on planchets and the radioactivity counted. Samples from the origin, solvent front and intermediate zones were also counted. Identification of the phospholipids was made by simultaneous chromatography of purified M. lysodeikticus phospholipids kindly supplied by Dr. August De Siervo. Polyacrylamide gel dectrophoresis Polyacrylamide gel electrophoresis was carried out using standard procedures employed in this laboratory13, la. Samples were adjusted to 1-2 mg pro¢ein/ml and o.15-o.18 ml applied to each column. Chemical and enzymatic assays Protein was determined by the method of Lowry et al. 15. ATPase (EC 3.6.1.3) was assayed according to Mufioz et al. 16 and NADH dehydrogenase (EC 1.6.99.3) was assayed according to Nachbar and Salton 17.

Biochimica et Biophysica Acta (BBA) - Biomembranes, 1973

I. Membrane-incorporated glucosamine does not seem to be covalently attached to membrane proteins... more I. Membrane-incorporated glucosamine does not seem to be covalently attached to membrane proteins as shown by polyacrylamide gel electrophoresis of native and pronase-digested membranes. 2. Membrane-incorporated glucosamine is readily released from membrane lipid and protein by brief sonication as shown by sucrose density gradient analysis. 3. Membrane-incorporated glucosamine is present in a polyme~c form, as shown by comparing column elution profiles for free and membrane-bound glucosamine. 4. It is concluded that the polymer is weakly associated with the membrane and may reside on or near the exterior surface.

Biochimica et Biophysica Acta (BBA) - Biomembranes, 1968

Small lipoprotein aggregates are formed when sodium dodecyl sulfate-solubilized membrane componen... more Small lipoprotein aggregates are formed when sodium dodecyl sulfate-solubilized membrane components are dialyzed against buffer in the absence of divalent cations. These aggregates sediment as a single peak in analytical ultracentrifugation, and their behavior on sucrose density gradient centrifugation indicates that the lipid is bound to the protein. Upon dialysis against buffer containing 0.02 M Mg 2+, the small pieces further aggregate to yield structures having a membrane-like appearance in the electron microscope. The collection of larger aggregates has been designated Mr' (reaggregated membrane prime) and has a buoyant density of i.I8 ~ o.o5 g/cm a. Mr' is similar to the original membrane in having the same protein to lipid ratio and the same magnesium to protein ratio. * Part of this material has been submitted in partial fulfihnent of the requirements for the Ph.D. degree at Yale University.