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Research paper thumbnail of Functional expression of five Arabidopsis fatty acyl-CoA reductase genes in Escherichia coli

Journal of Plant Physiology, 2009

Very long chain primary alcohols are significant components in cuticle waxes of plants. Fatty acy... more Very long chain primary alcohols are significant components in cuticle waxes of plants. Fatty acyl-CoA reductases (FARs) catalyze the formation of a fatty alcohol from an acyl-CoA. The Arabidopsis (Arabidopsis thaliana) genome contains eight genes homologous to FAR genes from jojoba (Simmondsia chinensis), silk moth, wheat and mouse. Expression of six Arabidopsis FAR homologs in Escherichia coli resulted in production of alcohols from endogenous E. coli fatty acids by five of these genes, confirming that they encode for FAR enzymes. Only a truncated splicing version of the sixth gene was found, and this gene yielded a protein with no FAR activity. The five functional FAR enzymes yielded distinctly different compositions of fatty alcohols when expressed in E. coli, indicating that the different enzymes may be involved in the production of different types of alcohols in plant cells.

Research paper thumbnail of Foods and Nutrition Nutrition Considerations in Aquaculture: The Importance of Omega-3 Fatty Acids in Fish Development and Human Health Molecular Biosciences and Bioengineering, College of Tropical Agriculture and Human Resources, 3 Aquatic Feeds and Nutrition Department, Oceanic Institute

808-956-7036. The university is an equal opportunity/affirmative action institution providing pro... more 808-956-7036. The university is an equal opportunity/affirmative action institution providing programs and services to the people of Hawai'i without regard to race, sex, gender identity and expression, age, religion, color, national origin, ancestry, disability, marital status, arrest and court record, sexual orientation, or status as a covered veteran. Find CTAHR publications at www.ctahr.hawaii.edu/freepubs.

Research paper thumbnail of Functional expression of five Arabidopsis fatty acyl-CoA reductase genes in Escherichia coli

Journal of Plant Physiology, 2009

Very long chain primary alcohols are significant components in cuticle waxes of plants. Fatty acy... more Very long chain primary alcohols are significant components in cuticle waxes of plants. Fatty acyl-CoA reductases (FARs) catalyze the formation of a fatty alcohol from an acyl-CoA. The Arabidopsis (Arabidopsis thaliana) genome contains eight genes homologous to FAR genes from jojoba (Simmondsia chinensis), silk moth, wheat and mouse. Expression of six Arabidopsis FAR homologs in Escherichia coli resulted in production of alcohols from endogenous E. coli fatty acids by five of these genes, confirming that they encode for FAR enzymes. Only a truncated splicing version of the sixth gene was found, and this gene yielded a protein with no FAR activity. The five functional FAR enzymes yielded distinctly different compositions of fatty alcohols when expressed in E. coli, indicating that the different enzymes may be involved in the production of different types of alcohols in plant cells.

Research paper thumbnail of Foods and Nutrition Nutrition Considerations in Aquaculture: The Importance of Omega-3 Fatty Acids in Fish Development and Human Health Molecular Biosciences and Bioengineering, College of Tropical Agriculture and Human Resources, 3 Aquatic Feeds and Nutrition Department, Oceanic Institute

808-956-7036. The university is an equal opportunity/affirmative action institution providing pro... more 808-956-7036. The university is an equal opportunity/affirmative action institution providing programs and services to the people of Hawai'i without regard to race, sex, gender identity and expression, age, religion, color, national origin, ancestry, disability, marital status, arrest and court record, sexual orientation, or status as a covered veteran. Find CTAHR publications at www.ctahr.hawaii.edu/freepubs.

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