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Research paper thumbnail of Molecular inhibition of phospholipase cgamma signaling abrogates DU-145 prostate tumor cell invasion

Clinical cancer research : an official journal of the American Association for Cancer Research, 1997

Up-regulated signaling from the epidermal growth factor receptor (EGFR) has been correlated with ... more Up-regulated signaling from the epidermal growth factor receptor (EGFR) has been correlated with tumor invasion and metastasis in numerous human neoplasias. Recently, we have demonstrated that increased levels of EGFR promote the invasiveness of human prostate carcinoma DU-145 cells. However, the intracellular signaling pathway responsible for this enhanced tumor invasiveness has not been identified. We postulated that increased cell motility signaled via phospholipase Cgamma (PLCgamma) activation was critical for tumor invasiveness. Highly invasive DU-145 cells engineered to overexpress the EGFR were stably transfected with a dominant-negative fragment of PLCgamma from the Z-region (PLCz) or with irrelevant peptide minigenes. PLCz was expressed only in the appropriate transfectant lines, with a concomitant decrease in inositol phosphate generation. The transfectant cell lines all formed tumors when inoculated into the peritoneal cavity of athymic mice. Tumors from the cells express...

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Research paper thumbnail of Luteinising hormone releasing hormone analogue for prostatic cancer

BMJ, 1985

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Research paper thumbnail of Luteinizing Hormone-releasing Hormone Agonist Limits DU-145 Prostate Cancer Growth by Attenuating Epidermal Growth Factor Receptor Signaling

Clinical Cancer Research an Official Journal of the American Association For Cancer Research, Apr 1, 2002

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Research paper thumbnail of Racial Disparities and Preventive Measures to Renal Cell Carcinoma

International journal of environmental research and public health, Jan 28, 2018

Kidney cancer ranks among the top 10 cancers in the United States. Although it affects both male ... more Kidney cancer ranks among the top 10 cancers in the United States. Although it affects both male and female populations, it is more common in males. The prevalence rate of renal cell carcinoma (RCC), which represents about 85% of kidney cancers, has been increasing gradually in many developed countries. Family history has been considered as one of the most relevant risk factors for kidney cancer, although most forms of an inherited predisposition for RCC only account for less than four percent. Lifestyle and other factors such as occupational exposure, high blood pressure, poor diet, and heavy cigarette smoking are highly associated with its incidence and mortality rates. In the United States, White populations have the lowest prevalence of RCC compared to other ethnic groups, while Black Americans suffer disproportionally from the adverse effects of RCC. Hence, this review article aims at identifying the major risk factors associated with RCC and highlighting the new therapeutic ap...

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Research paper thumbnail of Pittsburgh-Tuskegee Prostate Training Program

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Research paper thumbnail of IP-10 fragment is the functional motif that blocks endothelial cell motility and vessel formation

The Faseb Journal, Apr 1, 2009

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Research paper thumbnail of Serum-Free Culture of Enriched Mouse Anterior and Ventral Prostatic Epithelial Cells in Collagen Gel

In Vitro Cellular Developmental Biology, Aug 1, 1990

Sustained growth of mouse ventral and anterior prostatic epithelial cells embedded within collage... more Sustained growth of mouse ventral and anterior prostatic epithelial cells embedded within collagen gel matrix was achieved in a serum-free medium composed of Dulbecco's modified Eagle's medium and Ham's F12 medium, 1:1 (vol/vol), supplemented with bovine serum albumin fraction V, epidermal growth factor, transferrin, cholera toxin, prolactin, 5 alpha-dihydrotestosterone, cortisol, putrescine, fibroblast growth factor, and a trace element mixture. Three-dimensional growth of prostatic epithelial cells occurred inside the collagen gel matrix. This serum-free medium allowed cell growth greater than sevenfold over 10 d in culture. Tissue recombination and cell culture techniques were integrated to demonstrate that cultured cells retained prostatic characteristics. Following 10 d of culture, epithelial colonies from mouse ventral and anterior prostatic epithelial cell cultures were isolated and combined with rat fetal urogenital sinus mesenchyme and grown for 4 wk under the renal capsule of intact athymic male mice. These tissue recombinants showed distinctive prostatic histologic characteristics (alveoli and ducts lined with cuboidal or columnar epithelium surrounded by stroma). When histologic sections of recombinants were stained with the Hoechst 33258, epithelial cells of mouse origin were distinguishable from stromal cells of rat origin.

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Research paper thumbnail of Chemokine Derived Peptides and Uses for Chronic Wound and Angiogenesis Inhibition Treatments

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Research paper thumbnail of miRNA 26a EXPRESSION IN A NOVEL PANEL OF AFRICAN AMERICAN PROSTATE CANCER CELL LINES

Ethnicity Disease, 2010

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Research paper thumbnail of Lytic peptides having anti-proliferative activity against prostate cancer cells

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Research paper thumbnail of Biomarkers for Breast Cancer Patients

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Research paper thumbnail of Immortalized Human Prostate Cell Lines

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Research paper thumbnail of Chemokine derived peptides that bind with chemokine receptor CXCR3 and uses for chronic wound and angiogenesis inhibition treatments

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Research paper thumbnail of Kaiso: A Key Regulator in EMT and Cancer Progression

The Faseb Journal, Apr 1, 2012

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Research paper thumbnail of Uses of phospholipase C inhibitors

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Research paper thumbnail of Abstract 3675: Double receptor targeting multifunctional iron oxide nanoparticles drug delivery system for the treatment and imaging of prostate cancer

Cancer Research, 2015

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Research paper thumbnail of An IP-10 (CXCL10)-Derived Peptide Inhibits Angiogenesis

PLoS ONE, 2012

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Research paper thumbnail of Development of the Polio Vaccine: A Historical Perspective of Tuskegee University’s Role in Mass Production and Distribution of HeLa Cells

Journal of Health Care for the Poor and Underserved, 2012

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Research paper thumbnail of In memory of Professor Howard A. Bern

General and Comparative Endocrinology, 2012

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Research paper thumbnail of Double-receptor-targeting multifunctional iron oxide nanoparticles drug delivery system for the treatment and imaging of prostate cancer

International Journal of Nanomedicine

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Research paper thumbnail of Molecular inhibition of phospholipase cgamma signaling abrogates DU-145 prostate tumor cell invasion

Clinical cancer research : an official journal of the American Association for Cancer Research, 1997

Up-regulated signaling from the epidermal growth factor receptor (EGFR) has been correlated with ... more Up-regulated signaling from the epidermal growth factor receptor (EGFR) has been correlated with tumor invasion and metastasis in numerous human neoplasias. Recently, we have demonstrated that increased levels of EGFR promote the invasiveness of human prostate carcinoma DU-145 cells. However, the intracellular signaling pathway responsible for this enhanced tumor invasiveness has not been identified. We postulated that increased cell motility signaled via phospholipase Cgamma (PLCgamma) activation was critical for tumor invasiveness. Highly invasive DU-145 cells engineered to overexpress the EGFR were stably transfected with a dominant-negative fragment of PLCgamma from the Z-region (PLCz) or with irrelevant peptide minigenes. PLCz was expressed only in the appropriate transfectant lines, with a concomitant decrease in inositol phosphate generation. The transfectant cell lines all formed tumors when inoculated into the peritoneal cavity of athymic mice. Tumors from the cells express...

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Research paper thumbnail of Luteinising hormone releasing hormone analogue for prostatic cancer

BMJ, 1985

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Luteinizing Hormone-releasing Hormone Agonist Limits DU-145 Prostate Cancer Growth by Attenuating Epidermal Growth Factor Receptor Signaling

Clinical Cancer Research an Official Journal of the American Association For Cancer Research, Apr 1, 2002

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Racial Disparities and Preventive Measures to Renal Cell Carcinoma

International journal of environmental research and public health, Jan 28, 2018

Kidney cancer ranks among the top 10 cancers in the United States. Although it affects both male ... more Kidney cancer ranks among the top 10 cancers in the United States. Although it affects both male and female populations, it is more common in males. The prevalence rate of renal cell carcinoma (RCC), which represents about 85% of kidney cancers, has been increasing gradually in many developed countries. Family history has been considered as one of the most relevant risk factors for kidney cancer, although most forms of an inherited predisposition for RCC only account for less than four percent. Lifestyle and other factors such as occupational exposure, high blood pressure, poor diet, and heavy cigarette smoking are highly associated with its incidence and mortality rates. In the United States, White populations have the lowest prevalence of RCC compared to other ethnic groups, while Black Americans suffer disproportionally from the adverse effects of RCC. Hence, this review article aims at identifying the major risk factors associated with RCC and highlighting the new therapeutic ap...

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Research paper thumbnail of Pittsburgh-Tuskegee Prostate Training Program

Bookmarks Related papers MentionsView impact

Research paper thumbnail of IP-10 fragment is the functional motif that blocks endothelial cell motility and vessel formation

The Faseb Journal, Apr 1, 2009

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Serum-Free Culture of Enriched Mouse Anterior and Ventral Prostatic Epithelial Cells in Collagen Gel

In Vitro Cellular Developmental Biology, Aug 1, 1990

Sustained growth of mouse ventral and anterior prostatic epithelial cells embedded within collage... more Sustained growth of mouse ventral and anterior prostatic epithelial cells embedded within collagen gel matrix was achieved in a serum-free medium composed of Dulbecco's modified Eagle's medium and Ham's F12 medium, 1:1 (vol/vol), supplemented with bovine serum albumin fraction V, epidermal growth factor, transferrin, cholera toxin, prolactin, 5 alpha-dihydrotestosterone, cortisol, putrescine, fibroblast growth factor, and a trace element mixture. Three-dimensional growth of prostatic epithelial cells occurred inside the collagen gel matrix. This serum-free medium allowed cell growth greater than sevenfold over 10 d in culture. Tissue recombination and cell culture techniques were integrated to demonstrate that cultured cells retained prostatic characteristics. Following 10 d of culture, epithelial colonies from mouse ventral and anterior prostatic epithelial cell cultures were isolated and combined with rat fetal urogenital sinus mesenchyme and grown for 4 wk under the renal capsule of intact athymic male mice. These tissue recombinants showed distinctive prostatic histologic characteristics (alveoli and ducts lined with cuboidal or columnar epithelium surrounded by stroma). When histologic sections of recombinants were stained with the Hoechst 33258, epithelial cells of mouse origin were distinguishable from stromal cells of rat origin.

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Chemokine Derived Peptides and Uses for Chronic Wound and Angiogenesis Inhibition Treatments

Bookmarks Related papers MentionsView impact

Research paper thumbnail of miRNA 26a EXPRESSION IN A NOVEL PANEL OF AFRICAN AMERICAN PROSTATE CANCER CELL LINES

Ethnicity Disease, 2010

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Lytic peptides having anti-proliferative activity against prostate cancer cells

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Biomarkers for Breast Cancer Patients

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Immortalized Human Prostate Cell Lines

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Chemokine derived peptides that bind with chemokine receptor CXCR3 and uses for chronic wound and angiogenesis inhibition treatments

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Kaiso: A Key Regulator in EMT and Cancer Progression

The Faseb Journal, Apr 1, 2012

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Uses of phospholipase C inhibitors

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Abstract 3675: Double receptor targeting multifunctional iron oxide nanoparticles drug delivery system for the treatment and imaging of prostate cancer

Cancer Research, 2015

Bookmarks Related papers MentionsView impact

Research paper thumbnail of An IP-10 (CXCL10)-Derived Peptide Inhibits Angiogenesis

PLoS ONE, 2012

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Development of the Polio Vaccine: A Historical Perspective of Tuskegee University’s Role in Mass Production and Distribution of HeLa Cells

Journal of Health Care for the Poor and Underserved, 2012

Bookmarks Related papers MentionsView impact

Research paper thumbnail of In memory of Professor Howard A. Bern

General and Comparative Endocrinology, 2012

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Double-receptor-targeting multifunctional iron oxide nanoparticles drug delivery system for the treatment and imaging of prostate cancer

International Journal of Nanomedicine

Bookmarks Related papers MentionsView impact

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