Tiziana Catone - Academia.edu (original) (raw)
Papers by Tiziana Catone
Abstact. Trout samples with identical genetic origin, growing in different environment conditions... more Abstact. Trout samples with identical genetic origin, growing in different environment conditions (temperature of water) and fed with diets varying in the lipid content were collected from two breeding farms and sensorly analysed by a panel composed of 12 assessors. Sensory results were analysed and compared with chemical measurements by univariate and multivariate statistical techniques. The analyses show an essential role of the water temperature: individuals bred at lower water temperature, showed higher mean values for sensorial attributes considered a tool for quality evaluation. Diet showed an influence on the body lipid content.
, Preference and perception of ten fish products marketed as fresh , frozen and pre-cooked were i... more , Preference and perception of ten fish products marketed as fresh , frozen and pre-cooked were investigated on regular consumers of fish. In a first interview consumers were presented with photographs of the products and the technique of Repertory Grid was used to elucidate relevant constructs for the products. This qualitative phase was useful for the construction of personalised questionnaires. In a second interview the surveyed rating of liking and sensory and cognitive attributes and intention to buy was investigated. The structure of data was statistically analysed by Generalised Procrustes Analysis (GPA) to get an indication of consumer perception and thinking. Principal Component Analysis (PCA) was used to identify possible subgroups of consumers with different preferences. The study emphasise both sensory and attitudinal characteristics. 10 produits a base de poissons, frais, congele ou pre-cuit, ont ete testes: dans un premier temps, des photographies des produits ont ete presentees au testeurs, qui sont des consommateurs reguliers de poisson. Dans un second temps, une analyse sensorielle descriptive et les intentions d'acheter ont ete observees. Une analyse statistique a ete effectuee pour evaluer la perception du consommateur. L'analyse des composantes principales a permis de distinguer les sous groupes de consommateurs avec des preferences differentes.
Thermochimica Acta - THERMOCHIM ACTA, 2004
With thermogravimetric analysis (TGA) it is possible to have an estimation of vegetable oil resis... more With thermogravimetric analysis (TGA) it is possible to have an estimation of vegetable oil resistance to oxidation, having a measure of weight gain percent (Δ%) due to oxygen caption of oil sample during the oxidation, and of initial and final oxidation temperatures (Ti and Tf). The following samples were examined: extra-virgin olive oil, olive oil, sunflower seed oil, soybean oil, maize oil, peanut oil, rapeseed oil, grape seed oil, hazelnut oil, rice oil and four different kinds of oils for frying. An assessment of data obtained so far makes it possible to point out some interesting outcomes. With regard to weight gain percent, the peanut, rice and extra-virgin olive oils showed the lowest values, whereas the highest one has been detected for one of the oils for frying. As concerns the initial oxidation temperature the rice oil has displayed the maximum value, whereas the same oil for frying above said the minimum. The experimental approach and analytical method developed in this...
Reproductive toxicology (Elmsford, N.Y.), Jan 4, 2017
Children show unique features concerning chemical hazards and risks, due to different exposure sc... more Children show unique features concerning chemical hazards and risks, due to different exposure scenarios, age-related metabolic capacity and biological susceptibility linked to post-natal development. Chemical Regulatory frameworks state the need of children risk characterization. Current testing guidelines covering post-natal development are not routinely required by regulatory applications other than pesticides and biocides. Juvenile toxicity studies are foreseen for paediatric drugs: the toxicological repeated-dose tests don't allow accurate evaluations of effects upon direct exposure of immature organism. The paper discusses a testing approach aimed to address regulatory requirements of chemical hazard identification/characterization in a children-specific perspective. Juvenile toxicity test could be performed primarily on chemicals that may have relevant modes of action and/or age-related toxicokinetic differences and/or lead to important children exposure. This could be pu...
Annali dell'Istituto superiore di sanità, 2011
The CLP Regulation implements in the EU the UN Globally Harmonised System of Classification and L... more The CLP Regulation implements in the EU the UN Globally Harmonised System of Classification and Labelling applying the "building block approach", that is taking on board the hazard classes and categories which are close to the existing EU system in order to maintain the level of protection of human health and environment. This Regulation applies to all substances and mixtures placed on the market and besides to classification, packaging and labelling it provides for the notification of the classification and labelling of substances to the Classification & Labelling Inventory established by ECHA. It came into force on 20 January 2009 but a transitional period is foreseen until 1 June 2015 for the full application. At the end of this period the "substance" and "preparation" Directives (respectively 67/548/EEC and 99/45/EC) will be repealed.
Toxicology in Vitro, 2007
Clorpyrifos (CPF), one of the most widely used organophosphorothionate pesticide can be detected ... more Clorpyrifos (CPF), one of the most widely used organophosphorothionate pesticide can be detected as residues in food and drinking water; therefore the oral route is the major route of exposure for the general population, including children, following household use of this insecticide. Aim of this work was to investigate the possible acute cytotoxic effects of CPF on intestine and the integrity of the epithelial barrier, using Caco-2/TC7 cells as intestinal in vitro model. High level of CPF found inside the cells, corresponding to about 80% of the nominal concentration tested (30, 50 and 250 lM), chosen as representative of the concentrations attainable in the intestinal lumen after actual levels of human oral exposure. In these conditions, no cytotoxicity in terms of cellular viability was observed. However, at the highest CPF nominal concentration (250 lM) the impairment of barrier integrity was evidenced, due only to the parent compound, since no CPF metabolites could be detected in our experimental conditions. CPF itself was demonstrated to interfere with the tight junction on this in vitro model of epithelial intestinal cells, altering the barrier integrity and very likely the absorption of other co-administered chemicals.
Toxicology in Vitro, 2011
Chemico-Biological Interactions, 2010
Semicarbazide (SEM) is a by-product of the blowing agent azodicarbonamide, present in glass jar-s... more Semicarbazide (SEM) is a by-product of the blowing agent azodicarbonamide, present in glass jar-sealed foodstuffs mainly baby foods. The pleiotropic in vivo SEM toxicological effects suggested to explore its possible role as endocrine modulator. Endocrine effects of SEM were assessed in vivo in male and female rats after oral administration for 28 days at 0, 40, 75, 140 mg/kg bw pro die during the juvenile period. Vaginal opening and preputial separation were recorded. Concentration of sex steroid in blood, the ex vivo hepatic aromatase activity and testosterone catabolism were detected. The in vitro approach to test SEM role as (anti)estrogen or N-methyl-d-aspartate receptors (NMDARs)-(anti)agonist included different assays: yeast estrogenicity, MCF-7 proliferation, stimulation of the alkaline phosphatase activity in Ishikawa cells and LNCaP-based NMDAR interference assay. In vivo SEM-treated female rats showed delayed vaginal opening at all tested doses, whereas in males preputial separation was anticipated at SEM 40 and 75 mg/kg and delayed at 140 mg/kg, the latter effect probably due to the significantly decreased body weight gain seen at the higher dose in both sexes. Serum estrogen levels were dose-dependently reduced in treated females, whereas dehydrotestosterone serum levels were also decreased but a clear dose-response was not evidenced. Testosterone catabolism was altered in a gender-related way, aromatase activity was increased in treated males at 75 and 140 mg/kg and in females in all dose groups. In the three estradiol-competitive assays, SEM showed a weak anti-estrogenic activity, whereas in the LNCaP-based NMDAR interference assay SEM activity resembled MK-801 antagonist effect. SEM appeared to act as an endocrine disrupter showing multiple and gender specific mechanisms of action(s). A possible cascade-mechanism of SEM on reproductive signalling pathways may be hypothesized. Such in vivo-in vitro approach appeared to be an useful tool to highlight SEM activity on endocrine homeostasis.
Journal of food protection
Although rabbit meat production represents a very small percentage of the world meat market, this... more Although rabbit meat production represents a very small percentage of the world meat market, this percentage has been growing continuously during the last 30 years. Rabbit is considered a minor food species, and therefore no drugs are specifically registered for this animal. This situation encourages rabbit farmers to make off-label use of antibacterial drugs authorized for food-producing animal species other than rabbits. In the present study, the distribution and elimination of the fluoroquinolone antibacterial agent marbofloxacin in rabbit muscle, liver, and kidney was investigated. Marbofloxacin was chosen as a representative of a new generation of antibacterial drugs active against most gram-positive and gram-negative bacteria and mycoplasms; it is well tolerated and has short elimination times in bovine and swine species. Rabbits were treated with marbofloxacin at 2 mg kg of body weight(-1) for 5 days. Residual concentrations in liver, kidney, and muscle tissues were determine...
Regulatory Toxicology and Pharmacology, 2012
Regulatory Toxicology and Pharmacology, 2012
Abstact. Trout samples with identical genetic origin, growing in different environment conditions... more Abstact. Trout samples with identical genetic origin, growing in different environment conditions (temperature of water) and fed with diets varying in the lipid content were collected from two breeding farms and sensorly analysed by a panel composed of 12 assessors. Sensory results were analysed and compared with chemical measurements by univariate and multivariate statistical techniques. The analyses show an essential role of the water temperature: individuals bred at lower water temperature, showed higher mean values for sensorial attributes considered a tool for quality evaluation. Diet showed an influence on the body lipid content.
, Preference and perception of ten fish products marketed as fresh , frozen and pre-cooked were i... more , Preference and perception of ten fish products marketed as fresh , frozen and pre-cooked were investigated on regular consumers of fish. In a first interview consumers were presented with photographs of the products and the technique of Repertory Grid was used to elucidate relevant constructs for the products. This qualitative phase was useful for the construction of personalised questionnaires. In a second interview the surveyed rating of liking and sensory and cognitive attributes and intention to buy was investigated. The structure of data was statistically analysed by Generalised Procrustes Analysis (GPA) to get an indication of consumer perception and thinking. Principal Component Analysis (PCA) was used to identify possible subgroups of consumers with different preferences. The study emphasise both sensory and attitudinal characteristics. 10 produits a base de poissons, frais, congele ou pre-cuit, ont ete testes: dans un premier temps, des photographies des produits ont ete presentees au testeurs, qui sont des consommateurs reguliers de poisson. Dans un second temps, une analyse sensorielle descriptive et les intentions d'acheter ont ete observees. Une analyse statistique a ete effectuee pour evaluer la perception du consommateur. L'analyse des composantes principales a permis de distinguer les sous groupes de consommateurs avec des preferences differentes.
Thermochimica Acta - THERMOCHIM ACTA, 2004
With thermogravimetric analysis (TGA) it is possible to have an estimation of vegetable oil resis... more With thermogravimetric analysis (TGA) it is possible to have an estimation of vegetable oil resistance to oxidation, having a measure of weight gain percent (Δ%) due to oxygen caption of oil sample during the oxidation, and of initial and final oxidation temperatures (Ti and Tf). The following samples were examined: extra-virgin olive oil, olive oil, sunflower seed oil, soybean oil, maize oil, peanut oil, rapeseed oil, grape seed oil, hazelnut oil, rice oil and four different kinds of oils for frying. An assessment of data obtained so far makes it possible to point out some interesting outcomes. With regard to weight gain percent, the peanut, rice and extra-virgin olive oils showed the lowest values, whereas the highest one has been detected for one of the oils for frying. As concerns the initial oxidation temperature the rice oil has displayed the maximum value, whereas the same oil for frying above said the minimum. The experimental approach and analytical method developed in this...
Reproductive toxicology (Elmsford, N.Y.), Jan 4, 2017
Children show unique features concerning chemical hazards and risks, due to different exposure sc... more Children show unique features concerning chemical hazards and risks, due to different exposure scenarios, age-related metabolic capacity and biological susceptibility linked to post-natal development. Chemical Regulatory frameworks state the need of children risk characterization. Current testing guidelines covering post-natal development are not routinely required by regulatory applications other than pesticides and biocides. Juvenile toxicity studies are foreseen for paediatric drugs: the toxicological repeated-dose tests don't allow accurate evaluations of effects upon direct exposure of immature organism. The paper discusses a testing approach aimed to address regulatory requirements of chemical hazard identification/characterization in a children-specific perspective. Juvenile toxicity test could be performed primarily on chemicals that may have relevant modes of action and/or age-related toxicokinetic differences and/or lead to important children exposure. This could be pu...
Annali dell'Istituto superiore di sanità, 2011
The CLP Regulation implements in the EU the UN Globally Harmonised System of Classification and L... more The CLP Regulation implements in the EU the UN Globally Harmonised System of Classification and Labelling applying the "building block approach", that is taking on board the hazard classes and categories which are close to the existing EU system in order to maintain the level of protection of human health and environment. This Regulation applies to all substances and mixtures placed on the market and besides to classification, packaging and labelling it provides for the notification of the classification and labelling of substances to the Classification & Labelling Inventory established by ECHA. It came into force on 20 January 2009 but a transitional period is foreseen until 1 June 2015 for the full application. At the end of this period the "substance" and "preparation" Directives (respectively 67/548/EEC and 99/45/EC) will be repealed.
Toxicology in Vitro, 2007
Clorpyrifos (CPF), one of the most widely used organophosphorothionate pesticide can be detected ... more Clorpyrifos (CPF), one of the most widely used organophosphorothionate pesticide can be detected as residues in food and drinking water; therefore the oral route is the major route of exposure for the general population, including children, following household use of this insecticide. Aim of this work was to investigate the possible acute cytotoxic effects of CPF on intestine and the integrity of the epithelial barrier, using Caco-2/TC7 cells as intestinal in vitro model. High level of CPF found inside the cells, corresponding to about 80% of the nominal concentration tested (30, 50 and 250 lM), chosen as representative of the concentrations attainable in the intestinal lumen after actual levels of human oral exposure. In these conditions, no cytotoxicity in terms of cellular viability was observed. However, at the highest CPF nominal concentration (250 lM) the impairment of barrier integrity was evidenced, due only to the parent compound, since no CPF metabolites could be detected in our experimental conditions. CPF itself was demonstrated to interfere with the tight junction on this in vitro model of epithelial intestinal cells, altering the barrier integrity and very likely the absorption of other co-administered chemicals.
Toxicology in Vitro, 2011
Chemico-Biological Interactions, 2010
Semicarbazide (SEM) is a by-product of the blowing agent azodicarbonamide, present in glass jar-s... more Semicarbazide (SEM) is a by-product of the blowing agent azodicarbonamide, present in glass jar-sealed foodstuffs mainly baby foods. The pleiotropic in vivo SEM toxicological effects suggested to explore its possible role as endocrine modulator. Endocrine effects of SEM were assessed in vivo in male and female rats after oral administration for 28 days at 0, 40, 75, 140 mg/kg bw pro die during the juvenile period. Vaginal opening and preputial separation were recorded. Concentration of sex steroid in blood, the ex vivo hepatic aromatase activity and testosterone catabolism were detected. The in vitro approach to test SEM role as (anti)estrogen or N-methyl-d-aspartate receptors (NMDARs)-(anti)agonist included different assays: yeast estrogenicity, MCF-7 proliferation, stimulation of the alkaline phosphatase activity in Ishikawa cells and LNCaP-based NMDAR interference assay. In vivo SEM-treated female rats showed delayed vaginal opening at all tested doses, whereas in males preputial separation was anticipated at SEM 40 and 75 mg/kg and delayed at 140 mg/kg, the latter effect probably due to the significantly decreased body weight gain seen at the higher dose in both sexes. Serum estrogen levels were dose-dependently reduced in treated females, whereas dehydrotestosterone serum levels were also decreased but a clear dose-response was not evidenced. Testosterone catabolism was altered in a gender-related way, aromatase activity was increased in treated males at 75 and 140 mg/kg and in females in all dose groups. In the three estradiol-competitive assays, SEM showed a weak anti-estrogenic activity, whereas in the LNCaP-based NMDAR interference assay SEM activity resembled MK-801 antagonist effect. SEM appeared to act as an endocrine disrupter showing multiple and gender specific mechanisms of action(s). A possible cascade-mechanism of SEM on reproductive signalling pathways may be hypothesized. Such in vivo-in vitro approach appeared to be an useful tool to highlight SEM activity on endocrine homeostasis.
Journal of food protection
Although rabbit meat production represents a very small percentage of the world meat market, this... more Although rabbit meat production represents a very small percentage of the world meat market, this percentage has been growing continuously during the last 30 years. Rabbit is considered a minor food species, and therefore no drugs are specifically registered for this animal. This situation encourages rabbit farmers to make off-label use of antibacterial drugs authorized for food-producing animal species other than rabbits. In the present study, the distribution and elimination of the fluoroquinolone antibacterial agent marbofloxacin in rabbit muscle, liver, and kidney was investigated. Marbofloxacin was chosen as a representative of a new generation of antibacterial drugs active against most gram-positive and gram-negative bacteria and mycoplasms; it is well tolerated and has short elimination times in bovine and swine species. Rabbits were treated with marbofloxacin at 2 mg kg of body weight(-1) for 5 days. Residual concentrations in liver, kidney, and muscle tissues were determine...
Regulatory Toxicology and Pharmacology, 2012
Regulatory Toxicology and Pharmacology, 2012