Felicia Toma - Academia.edu (original) (raw)
Papers by Felicia Toma
Giardia lamblia and duodenal involvement
Bacteriologia, virusologia, parazitologia, epidemiologia (Bucharest, Romania : 1990), 2004
Giardia lamblia (GL) is one of the causes of diarrhoea. GL is a frequent parasite in our country.... more Giardia lamblia (GL) is one of the causes of diarrhoea. GL is a frequent parasite in our country. In the literature, endoscopic aspects of Giardia duodenitis are mentioned. Some authors tried even to make the diagnosis on endoscopic criteria.
Acta Marisiensis - Seria Medica
Interleukin-6 (IL-6) is a cytokine with pleiotropic effects that might also influence the virulen... more Interleukin-6 (IL-6) is a cytokine with pleiotropic effects that might also influence the virulence traits of some microorganisms, but its direct influence over Candida spp. is currently unknown. The objective of the study is to determine the influence of IL-6 (250 pg/ml) on the growth rate and biofilm formation of C. albicans, C. parapsilosis, C. krusei, C. auris, and C. guilliermondii, as well as to analyze the influence of this citokine on the expression of three virulence genes (ALS3, HSP70, SAP2), respectively on the germ tube formation ability of C. albicans. The influence of IL-6 on growth rate was assessed by incubating the fungal cells in presence of IL-6 for 48 hours and assessing the optical density of the samples at five timepoints. The biofilm production in presence of IL-6 was studied in microtiter plates, using crystal-violet assay, the intensity of biofilms being evaluated by spectrophotometry. The expression of ALS3, HSP70, and SAP2 in C. albicans was studied by RT-...
Bacterial etiology of the conditions associated with bacteremia/septicemia during the years 2006-2007
Bacteriologia, virusologia, parazitologia, epidemiologia (Bucharest, Romania : 1990)
Evaluation of the etiology of bacterial diseases associated with bacteremia/ sepsis in the Emerge... more Evaluation of the etiology of bacterial diseases associated with bacteremia/ sepsis in the Emergency County Hospital of Tg. Mureş during 2006-2007. Blood samples of 684 patients hospitalized in Emergency County Hospital of Tg. Mureş during January 2006 - December 2007 were analyzed. The isolated germs have been identified according to standard methods. The blood culture results were centralized and processed in electronic databases. In 2006 and 2007 the frequency of identified germs from blood cultures remained unchanged: the predominant germs are coagulase-negative staphylococci, followed by enterobacteria (Klebsiella spp prevailing), S. aureus (mostly methicillin-resistant (MRSA)) and other bacteria. The main bacteria isolated from blood culture are staphylococci and enterobacteria. In medical sections undertaking invasive procedures, the infections with methicillin-resistant staphylococci are common.
Case-Control Study of Epstein-Barr Viral Infection in Hodgkin's Disease
Summary The aim of this study is the assessment of Epstein Barr viral infection in patients with ... more Summary The aim of this study is the assessment of Epstein Barr viral infection in patients with Hodgkin’s disease. Our study is a prospective case- control study. We used serological methods such as ELISA technique to detect anti-EBV antibodies, anti-EBNA1 IgG and Epstein-Barr viral infection. We have evaluated 63 patients (40 men, 38 of which were from an urban area) with Hodgkin’s disease referring to the Târgu-Mure s 1 st Medical Clinic, and 63 controls. Epstein-Barr viral infection was serol ogically confirmed in 83.33% of Hodgkin’s lymphoma patients and 79.37% of controls. The study shows a statistically nonsignificant increase of Odds Ratio in all cases, da ta that confirms other existent studies. Statistical analysis on demographic criteria reveal ed high magnitudes of OR but statistically non-significant increases of infection in male popu lation and also in rural population.
[Re-emergent etiologies: Clostridium difficile]
Bacteriologia, virusologia, parazitologia, epidemiologia, 2007
Clostridium difficile-associated disease (CDAD) can range from uncomplicated diarrhea to sepsis a... more Clostridium difficile-associated disease (CDAD) can range from uncomplicated diarrhea to sepsis and even death. CDAD rates and severity are increasing, possibly due to a new strain. Transmission of C. difficile occurs primarily in health care facilities via the fecal-oral route following transient contamination of the hands of health care workers and patients; contamination of the patient care environment also plays an important role. Education of hospital staff appropriate diagnostic testing, early treatment, and measures for preventing spread of the pathogen could lead to decreasing the morbidity and mortality rates.
Purpose: Evaluation of Clostridium difficile infection involvement in the etiology of diarrheal d... more Purpose: Evaluation of Clostridium difficile infection involvement in the etiology of diarrheal disease. Material and methods: A retrospective study was conducted in the Laboratory of Infectious Diseases Clinic I Tirgu-Mure s, between 03.2009 and 12.2011. 6529 samples of feces were processed following the coproculture method. Rotavirus testing was performed by latex-aggluti nation. 1923 samples were processed following the ELISA technique, for Clostridium difficile, Campylobacter jejuni, and Giardialamblia. Results: Through the coproculture method, we reported a rate of 22.53% positive results. The highest percentage was found for Salmonella spp., E. coli and then Shigella spp. ELISA test was most frequently positive for Campylobacter spp. (4.47%), Giardia lamblia (2.65%), Clostridium difficile (1.25%). Conclusions: The most common positive diagnosis was for Campylobacter spp, followed by Giardialambli a, Salmonella spp., E. coli, and only then Rotavirus. Infection caused by Clostri...
[Cumulative antibiogram--means of following local resistance phenomena based on material gathered in the laboratory of microbiology]
Bacteriologia, virusologia, parazitologia, epidemiologia, 2008
The cumulative antibiogram (CA) represents a report which summarizes the susceptibility of common... more The cumulative antibiogram (CA) represents a report which summarizes the susceptibility of commonly isolated microorganisms to usual antibiotics in a defined period of time. We have evaluated the local resistance data during the year 2007 by generating CA from the bacteriology laboratory's database in Mures County Emergency Clinical Hospital for 3 categories of microorganisms: Gram-positive cocci, Gram-negative enteric rods and non-fermenters. A total of 1382 individual antibiotic susceptibility reports were analysed. Gram-positive cocci were susceptible 100% to glycopeptides and oxazolidinones. Fifty percent of S. aureus isolates were methicillin-resistant. More than 90% of S. aureus and MRSA (methicillin-resistant S. aureus) isolates were susceptible to trimethoprim-sulfamethoxazole. Gram-negative enteric rods were 100% susceptible to carbapenems. The susceptibility of Escherichia coli and Klebsiella pneumoniae to broad spectrum cephalosporins was 72% and 26%, respectively. Th...
The aim of the present study was to analyze the clo nal relations and virulence profile of clinic... more The aim of the present study was to analyze the clo nal relations and virulence profile of clinically r elevant MRSA strains collected during one year from pat ients admitted to clinical wards with high risk for nosocomial infections in a university hospital. One hundred and twenty two MRSA strains isolated from patients admitted to surgical and intensive care units during 2010 we re analyzed. Molecular characterization was perform ed using PFGE (pulsed-field gel electrophoresis), determinin g spa polymorphism and SCCmec type. The presence of genes encoding for virulence factors was assessed. W have identified 25 distinct pulsotypes clusterin g into 4 major clonal groups. Group A comprised 82% of MRSA strains (n=100), corresponding to spa type t030. Th ese strains harboured SCCmec type III and were positive for enterotoxin A genes. Fifteen percent of the st rains (n=18) belonged to group B representing spa types t 127, t015 and t321, carrying SCCmec type IV and gen es encoding for d...
[Intestinal parasitic diseases in children]
Bacteriologia, virusologia, parazitologia, epidemiologia, 2007
PURPOSE To compare the incidence of intestinal parasitosis between children with residence in urb... more PURPOSE To compare the incidence of intestinal parasitosis between children with residence in urban and rural areas: to compare the efficacy of parasitologic diagnostic methods. MATERIALS AND METHODS In our study we included two lots of children. The first lot consisted in 74 children from rural areas from which we collected 44 samples of feces and 55 samples for the "Scotch tape" test. The second lot consisted in 214 children from urban areas from which we collected 44 samples of feces. We examined each sample of feces by three different methods. The study was performed between April to June 2006. RESULTS The incidence of intestinal parasitosis increases in children from urban areas towards rural areas, and in children between 5 and 10 years. Ascariasis is the most frequent disease in both urban and rural areas. By examination of each fecal sample by three different methods, the number of positive cases increased. CONCLUSIONS The residence in rural areas and age between 5...
[Prevalence of tuberculosis in Mureş County, Romania, 2006-2008]
Revista medico-chirurgicala a Societatii de Medici si Naturalisti din Iasi, 2010
UNLABELLED The aim of this study is analysis of tuberculosis prevalence in Mures county for a per... more UNLABELLED The aim of this study is analysis of tuberculosis prevalence in Mures county for a period of three years (2006-2008). MATERIAL AND METHOD We monitored the prevalence of tuberculosis cases in the Mureş area recorded at Tg. Mureş TB Dispensary, the new cases and readmitted cases, chronic or abandonment, separately for children and adults, evolving for 2006-2008, data compared with national registration. RESULTS From 2006 to 2008, frequency of TB illness in our area decreased instead of the share of patients with positive results (BK+) which have increased, associated with an amplified risk. In our study the assessment during the three years showed a decrease of the total number of TB cases, instead of increased frequency of new cases of acute than those of chronic, increased cases of adults against children and cases of failed healing. Number of new cases of TB in Mureş urban areas, by years, showed the same decreasing trend for cities monitored, except Tg. Mureş where the ...
[Microbial sensitivity to ambazone (Faringosept) in pharyngeal samples from patients with acute infections of the upper respiratory tract]
Bacteriologia, virusologia, parazitologia, epidemiologia, 2007
Gastroenterology Insights, 2021
Diarrheal disease is still a major public health concern, as it is still considered an important ... more Diarrheal disease is still a major public health concern, as it is still considered an important cause of death in children under five years of age. A few decades ago, the detection of enteropathogenic E. coli was made by detecting the O, H, and K antigens, mostly by agglutination. The recent protocols recommend the molecular methods for diagnosing EPEC, as they can distinguish between typical and atypical EPEC by identifying the presence/absence of specific virulence factors. EPEC are defined as diarrheagenic strains of E. coli that can produce attaching and effacing lesions on the intestinal epithelium while being incapable of producing Shiga toxins and heat-labile or heat-stable enterotoxins. The ability of these strains to produce attaching and effacing lesions enable them to cause localized lesions by attaching tightly to the surface of the intestinal epithelial cells, disrupting the surfaces of the cells, thus leading to the effacement of the microvilli. EPEC are classified on...
[Chemoresistance of mycobacteria in a group of patients with tuberculosis from Mureş county]
Bacteriologia, virusologia, parazitologia, epidemiologia, 2007
AIM We studied tuberculosis drug-resistance cases related to risk factors involved, in Mureş dist... more AIM We studied tuberculosis drug-resistance cases related to risk factors involved, in Mureş district. METHODS We used clinical files of 91 patients with tuberculosis drug resistance from 2006 at all, diagnosed during 2002-2006 at the Ambulatory department of Tuberculosis Clinic in Tg. Mureş town and cities nearby. RESULTS. Mycobacterium drug resistance is more frequent at men (96.8%), because of multiple tuberculosis risk factors involved, also is more frequent in rural areas (52.8%) because of low access to medical services. Drug resistance rate have a direct growing related to number of treatments made before, at patients with therapeutic failure and chronic cases. In our study, first year was dominated by single drug resistance to NIH, with a decrease in 2004 and no cases in 2006; and also multi-drug resistance was placed on the first place by frequency in 2006. CONCLUSIONS We sustain the needs to intensify the general efforts on tuberculosis early diagnosis, also to increase th...
Antibiotics, 2021
Due to their high content of biomolecules, combined with silver’s well known antimicrobial potent... more Due to their high content of biomolecules, combined with silver’s well known antimicrobial potential, silver nanoparticles biosynthesized using spruce bark (AgNP SBEs) demonstrate antibacterial and antioxidant activity, making them a versatile option for developing new antimicrobial agents that might be used for medical treatment or as adjuvants for the classical agents. This study aims to analyze if silver nanoparticles (AgNPs) mediated by spruce bark extract (SBE) and silver salts (AgNP SBE Acetate, AgNP SBE Nitrate) presents antifungal activity against five different Candida spp., synergistic activity with fluconazole, and if they interact with some virulence factors of C. albicans. AgNP SBEs presented MICs (minimum inhibitory concentrations) for all the five tested Candida spp. AgNP SBEs inhibited the growth of C. parapsilosis, C. krusei, and C. guilliermondii, exerted synergistic activity with fluconazole for C. parapsilosis and C. guilliermondii, and inhibited biofilm producti...
Synthesis , Characterization and Microbiological Activity Evaluation of Two Silver Complexes with Norfloxacin
Silver complexes of norfloxacin (NOR) are interesting for therapeutic purposes due to possible sy... more Silver complexes of norfloxacin (NOR) are interesting for therapeutic purposes due to possible synergistic bactericidal and antifungal potential provided by the metal and fluoroquinolones (FQ). In order to obtain new compounds with superior antibacterial and antifungal properties, we synthesized two silver complexes of NOR. A multi-analytical approach was carried out by using elemental analysis (C, H, N), conductivity measurement, differential scanning calorimetry and spectroscopic methods (FT-IR, H-NMR, UV-VIS and PL). The screening of antibacterial activity and antifungal of the complexes was performed by determination of minimal inhibitory concentration (MIC) through microtiter broth dilution method.
Even if serotyping based on O antigens is still routinely used by most laboratories for the detec... more Even if serotyping based on O antigens is still routinely used by most laboratories for the detection of diarrheagenic Escherichia coli, this method can provide false-positive reactions, due to the high diversity of O antigens. Molecular methods represent a valuable tool that clarifies these situations. In the Bacteriology Laboratory of Mureș County Hospital, between May 2016 and July 2019, 160 diarrheagenic E. coli strains were isolated from children under 2 years old with diarrheic disease. The strains were identified as Shiga toxin-producing E. coli (STEC)/enteropathogenic Escherichia coli (EPEC) via agglutination with polyvalent sera. STEC strains were serotyped using monovalent sera for serogroup O157. Simplex PCR was performed on the strains to determine the presence of the hlyA gene, and, for the positive ones, the hemolytic activity was tested. Antibiotic susceptibility of the identified diarrheagenic E. coli strains was also investigated. STEC strains were the most frequent...
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[Detection of the sensitivity of some bacteria and fungi to the action of sodium phosphotungstate beta-Na6[P2W18O62].18 H2O]
Poliyoxometalales are a very special category of the chemical compounds that have multiply proper... more Poliyoxometalales are a very special category of the chemical compounds that have multiply properties and possibility to application in a lot of actual domain of the scientific research. One of the most practice application of the polyoxometalates with a special significance is their antibacterial and anti fungus actions. For this study, there have been used fungus, Gram-positive and Gram-negative bacteria, and the action of the polyoxometalates was tested simultaneously with the action of the specifically antibiotics for the studied bacteria. In order to determine the fungus and bacteria sensibility to the tested substance, there have been used three methods: two qualitative, diffusion methods (rondel and bucket methods) and one quantitative method (determination of the minimum inhibitory concentration-MIC). The results of the bucket method were similarly with ones obtained with the rondel method: only Staphylococcus aureus strains were sensitive to the sodium phosphotungstate. Usi...
[Evaluation of iron, fluoride and iodine concentrations in underground water in some counties in Romania]
AIM To compare the underground water mineral composition in iron, fluorine and iodine from differ... more AIM To compare the underground water mineral composition in iron, fluorine and iodine from different sources, with variable locations in Romania. METHODS It is a lab study based on Hanna Instruments C99 colorimeter used for our tests, that follows the levels of mineral parameters from water samples from several underground water supplie sources from 10 different romanian counties. RESULTS It were significant differences between sources from different counties, and also from different sources in the same county, regarding minerals levels in water and also comparing to admitted levels. The most various data were related to fluorine and iodine levels in water samples and risk of exposure by deficiency. CONCLUSION It is important to know the importance of supply water monitoring, to evaluate both the drinking water quality parameters and mineral composition, in order to estimate the influence on health status of long shut consumer population.
The increase of antibiotic resistant bacteria in lasts years resulted in limited options for trea... more The increase of antibiotic resistant bacteria in lasts years resulted in limited options for treatment of bacterial diseases. Escherichia coli is one of the most common Gram-negative bacterial pathogen and a cause of both community and hospital acquired infections. Medicinal plants are alternative rich sources of useful antibacterial agents. The antimicrobial activities of the spruce (Picea abies L.) bark polyphenolic extracts were examined for their capacity to inhibit the growth of Escherichia coli. Spruce bark extract was obtained by conventional aqueous extraction and with ultrasounds. The minimum inhibitory concentration was determined by microdilution method. The antibacterial effect of both extracts was strong against Escherichia coli. The antimicrobial effect of polyphenolic extracts on Escherichia coli was expressed at a concentration of 15 mg/ml. Antimicrobial activity of spruce bark compounds suggest a possible use of spruce bark in pharmaceutical preparations.
The aim of the present study was to analyze the clo nal relations and virulence profile of clinic... more The aim of the present study was to analyze the clo nal relations and virulence profile of clinically r elevant MRSA strains collected during one year from pat ients admitted to clinical wards with high risk for nosocomial infections in a university hospital. One hundred and twenty two MRSA strains isolated from patients admitted to surgical and intensive care units during 2010 we re analyzed. Molecular characterization was perform ed using PFGE (pulsed-field gel electrophoresis), determinin g spa polymorphism and SCCmec type. The presence of genes encoding for virulence factors was assessed. We have identified 25 distinct pulsotypes clusterin g into 4 major clonal groups. Group A comprised 82% of MRSA strains (n=100), corresponding to spa type t030. Th ese strains harboured SCCmec type III and were positive for enterotoxin A genes. Fifteen percent of the st rains (n=18) belonged to group B representing spa types t 127, t015 and t321, carrying SCCmec type IV and gen es encoding for ...
Giardia lamblia and duodenal involvement
Bacteriologia, virusologia, parazitologia, epidemiologia (Bucharest, Romania : 1990), 2004
Giardia lamblia (GL) is one of the causes of diarrhoea. GL is a frequent parasite in our country.... more Giardia lamblia (GL) is one of the causes of diarrhoea. GL is a frequent parasite in our country. In the literature, endoscopic aspects of Giardia duodenitis are mentioned. Some authors tried even to make the diagnosis on endoscopic criteria.
Acta Marisiensis - Seria Medica
Interleukin-6 (IL-6) is a cytokine with pleiotropic effects that might also influence the virulen... more Interleukin-6 (IL-6) is a cytokine with pleiotropic effects that might also influence the virulence traits of some microorganisms, but its direct influence over Candida spp. is currently unknown. The objective of the study is to determine the influence of IL-6 (250 pg/ml) on the growth rate and biofilm formation of C. albicans, C. parapsilosis, C. krusei, C. auris, and C. guilliermondii, as well as to analyze the influence of this citokine on the expression of three virulence genes (ALS3, HSP70, SAP2), respectively on the germ tube formation ability of C. albicans. The influence of IL-6 on growth rate was assessed by incubating the fungal cells in presence of IL-6 for 48 hours and assessing the optical density of the samples at five timepoints. The biofilm production in presence of IL-6 was studied in microtiter plates, using crystal-violet assay, the intensity of biofilms being evaluated by spectrophotometry. The expression of ALS3, HSP70, and SAP2 in C. albicans was studied by RT-...
Bacterial etiology of the conditions associated with bacteremia/septicemia during the years 2006-2007
Bacteriologia, virusologia, parazitologia, epidemiologia (Bucharest, Romania : 1990)
Evaluation of the etiology of bacterial diseases associated with bacteremia/ sepsis in the Emerge... more Evaluation of the etiology of bacterial diseases associated with bacteremia/ sepsis in the Emergency County Hospital of Tg. Mureş during 2006-2007. Blood samples of 684 patients hospitalized in Emergency County Hospital of Tg. Mureş during January 2006 - December 2007 were analyzed. The isolated germs have been identified according to standard methods. The blood culture results were centralized and processed in electronic databases. In 2006 and 2007 the frequency of identified germs from blood cultures remained unchanged: the predominant germs are coagulase-negative staphylococci, followed by enterobacteria (Klebsiella spp prevailing), S. aureus (mostly methicillin-resistant (MRSA)) and other bacteria. The main bacteria isolated from blood culture are staphylococci and enterobacteria. In medical sections undertaking invasive procedures, the infections with methicillin-resistant staphylococci are common.
Case-Control Study of Epstein-Barr Viral Infection in Hodgkin's Disease
Summary The aim of this study is the assessment of Epstein Barr viral infection in patients with ... more Summary The aim of this study is the assessment of Epstein Barr viral infection in patients with Hodgkin’s disease. Our study is a prospective case- control study. We used serological methods such as ELISA technique to detect anti-EBV antibodies, anti-EBNA1 IgG and Epstein-Barr viral infection. We have evaluated 63 patients (40 men, 38 of which were from an urban area) with Hodgkin’s disease referring to the Târgu-Mure s 1 st Medical Clinic, and 63 controls. Epstein-Barr viral infection was serol ogically confirmed in 83.33% of Hodgkin’s lymphoma patients and 79.37% of controls. The study shows a statistically nonsignificant increase of Odds Ratio in all cases, da ta that confirms other existent studies. Statistical analysis on demographic criteria reveal ed high magnitudes of OR but statistically non-significant increases of infection in male popu lation and also in rural population.
[Re-emergent etiologies: Clostridium difficile]
Bacteriologia, virusologia, parazitologia, epidemiologia, 2007
Clostridium difficile-associated disease (CDAD) can range from uncomplicated diarrhea to sepsis a... more Clostridium difficile-associated disease (CDAD) can range from uncomplicated diarrhea to sepsis and even death. CDAD rates and severity are increasing, possibly due to a new strain. Transmission of C. difficile occurs primarily in health care facilities via the fecal-oral route following transient contamination of the hands of health care workers and patients; contamination of the patient care environment also plays an important role. Education of hospital staff appropriate diagnostic testing, early treatment, and measures for preventing spread of the pathogen could lead to decreasing the morbidity and mortality rates.
Purpose: Evaluation of Clostridium difficile infection involvement in the etiology of diarrheal d... more Purpose: Evaluation of Clostridium difficile infection involvement in the etiology of diarrheal disease. Material and methods: A retrospective study was conducted in the Laboratory of Infectious Diseases Clinic I Tirgu-Mure s, between 03.2009 and 12.2011. 6529 samples of feces were processed following the coproculture method. Rotavirus testing was performed by latex-aggluti nation. 1923 samples were processed following the ELISA technique, for Clostridium difficile, Campylobacter jejuni, and Giardialamblia. Results: Through the coproculture method, we reported a rate of 22.53% positive results. The highest percentage was found for Salmonella spp., E. coli and then Shigella spp. ELISA test was most frequently positive for Campylobacter spp. (4.47%), Giardia lamblia (2.65%), Clostridium difficile (1.25%). Conclusions: The most common positive diagnosis was for Campylobacter spp, followed by Giardialambli a, Salmonella spp., E. coli, and only then Rotavirus. Infection caused by Clostri...
[Cumulative antibiogram--means of following local resistance phenomena based on material gathered in the laboratory of microbiology]
Bacteriologia, virusologia, parazitologia, epidemiologia, 2008
The cumulative antibiogram (CA) represents a report which summarizes the susceptibility of common... more The cumulative antibiogram (CA) represents a report which summarizes the susceptibility of commonly isolated microorganisms to usual antibiotics in a defined period of time. We have evaluated the local resistance data during the year 2007 by generating CA from the bacteriology laboratory's database in Mures County Emergency Clinical Hospital for 3 categories of microorganisms: Gram-positive cocci, Gram-negative enteric rods and non-fermenters. A total of 1382 individual antibiotic susceptibility reports were analysed. Gram-positive cocci were susceptible 100% to glycopeptides and oxazolidinones. Fifty percent of S. aureus isolates were methicillin-resistant. More than 90% of S. aureus and MRSA (methicillin-resistant S. aureus) isolates were susceptible to trimethoprim-sulfamethoxazole. Gram-negative enteric rods were 100% susceptible to carbapenems. The susceptibility of Escherichia coli and Klebsiella pneumoniae to broad spectrum cephalosporins was 72% and 26%, respectively. Th...
The aim of the present study was to analyze the clo nal relations and virulence profile of clinic... more The aim of the present study was to analyze the clo nal relations and virulence profile of clinically r elevant MRSA strains collected during one year from pat ients admitted to clinical wards with high risk for nosocomial infections in a university hospital. One hundred and twenty two MRSA strains isolated from patients admitted to surgical and intensive care units during 2010 we re analyzed. Molecular characterization was perform ed using PFGE (pulsed-field gel electrophoresis), determinin g spa polymorphism and SCCmec type. The presence of genes encoding for virulence factors was assessed. W have identified 25 distinct pulsotypes clusterin g into 4 major clonal groups. Group A comprised 82% of MRSA strains (n=100), corresponding to spa type t030. Th ese strains harboured SCCmec type III and were positive for enterotoxin A genes. Fifteen percent of the st rains (n=18) belonged to group B representing spa types t 127, t015 and t321, carrying SCCmec type IV and gen es encoding for d...
[Intestinal parasitic diseases in children]
Bacteriologia, virusologia, parazitologia, epidemiologia, 2007
PURPOSE To compare the incidence of intestinal parasitosis between children with residence in urb... more PURPOSE To compare the incidence of intestinal parasitosis between children with residence in urban and rural areas: to compare the efficacy of parasitologic diagnostic methods. MATERIALS AND METHODS In our study we included two lots of children. The first lot consisted in 74 children from rural areas from which we collected 44 samples of feces and 55 samples for the "Scotch tape" test. The second lot consisted in 214 children from urban areas from which we collected 44 samples of feces. We examined each sample of feces by three different methods. The study was performed between April to June 2006. RESULTS The incidence of intestinal parasitosis increases in children from urban areas towards rural areas, and in children between 5 and 10 years. Ascariasis is the most frequent disease in both urban and rural areas. By examination of each fecal sample by three different methods, the number of positive cases increased. CONCLUSIONS The residence in rural areas and age between 5...
[Prevalence of tuberculosis in Mureş County, Romania, 2006-2008]
Revista medico-chirurgicala a Societatii de Medici si Naturalisti din Iasi, 2010
UNLABELLED The aim of this study is analysis of tuberculosis prevalence in Mures county for a per... more UNLABELLED The aim of this study is analysis of tuberculosis prevalence in Mures county for a period of three years (2006-2008). MATERIAL AND METHOD We monitored the prevalence of tuberculosis cases in the Mureş area recorded at Tg. Mureş TB Dispensary, the new cases and readmitted cases, chronic or abandonment, separately for children and adults, evolving for 2006-2008, data compared with national registration. RESULTS From 2006 to 2008, frequency of TB illness in our area decreased instead of the share of patients with positive results (BK+) which have increased, associated with an amplified risk. In our study the assessment during the three years showed a decrease of the total number of TB cases, instead of increased frequency of new cases of acute than those of chronic, increased cases of adults against children and cases of failed healing. Number of new cases of TB in Mureş urban areas, by years, showed the same decreasing trend for cities monitored, except Tg. Mureş where the ...
[Microbial sensitivity to ambazone (Faringosept) in pharyngeal samples from patients with acute infections of the upper respiratory tract]
Bacteriologia, virusologia, parazitologia, epidemiologia, 2007
Gastroenterology Insights, 2021
Diarrheal disease is still a major public health concern, as it is still considered an important ... more Diarrheal disease is still a major public health concern, as it is still considered an important cause of death in children under five years of age. A few decades ago, the detection of enteropathogenic E. coli was made by detecting the O, H, and K antigens, mostly by agglutination. The recent protocols recommend the molecular methods for diagnosing EPEC, as they can distinguish between typical and atypical EPEC by identifying the presence/absence of specific virulence factors. EPEC are defined as diarrheagenic strains of E. coli that can produce attaching and effacing lesions on the intestinal epithelium while being incapable of producing Shiga toxins and heat-labile or heat-stable enterotoxins. The ability of these strains to produce attaching and effacing lesions enable them to cause localized lesions by attaching tightly to the surface of the intestinal epithelial cells, disrupting the surfaces of the cells, thus leading to the effacement of the microvilli. EPEC are classified on...
[Chemoresistance of mycobacteria in a group of patients with tuberculosis from Mureş county]
Bacteriologia, virusologia, parazitologia, epidemiologia, 2007
AIM We studied tuberculosis drug-resistance cases related to risk factors involved, in Mureş dist... more AIM We studied tuberculosis drug-resistance cases related to risk factors involved, in Mureş district. METHODS We used clinical files of 91 patients with tuberculosis drug resistance from 2006 at all, diagnosed during 2002-2006 at the Ambulatory department of Tuberculosis Clinic in Tg. Mureş town and cities nearby. RESULTS. Mycobacterium drug resistance is more frequent at men (96.8%), because of multiple tuberculosis risk factors involved, also is more frequent in rural areas (52.8%) because of low access to medical services. Drug resistance rate have a direct growing related to number of treatments made before, at patients with therapeutic failure and chronic cases. In our study, first year was dominated by single drug resistance to NIH, with a decrease in 2004 and no cases in 2006; and also multi-drug resistance was placed on the first place by frequency in 2006. CONCLUSIONS We sustain the needs to intensify the general efforts on tuberculosis early diagnosis, also to increase th...
Antibiotics, 2021
Due to their high content of biomolecules, combined with silver’s well known antimicrobial potent... more Due to their high content of biomolecules, combined with silver’s well known antimicrobial potential, silver nanoparticles biosynthesized using spruce bark (AgNP SBEs) demonstrate antibacterial and antioxidant activity, making them a versatile option for developing new antimicrobial agents that might be used for medical treatment or as adjuvants for the classical agents. This study aims to analyze if silver nanoparticles (AgNPs) mediated by spruce bark extract (SBE) and silver salts (AgNP SBE Acetate, AgNP SBE Nitrate) presents antifungal activity against five different Candida spp., synergistic activity with fluconazole, and if they interact with some virulence factors of C. albicans. AgNP SBEs presented MICs (minimum inhibitory concentrations) for all the five tested Candida spp. AgNP SBEs inhibited the growth of C. parapsilosis, C. krusei, and C. guilliermondii, exerted synergistic activity with fluconazole for C. parapsilosis and C. guilliermondii, and inhibited biofilm producti...
Synthesis , Characterization and Microbiological Activity Evaluation of Two Silver Complexes with Norfloxacin
Silver complexes of norfloxacin (NOR) are interesting for therapeutic purposes due to possible sy... more Silver complexes of norfloxacin (NOR) are interesting for therapeutic purposes due to possible synergistic bactericidal and antifungal potential provided by the metal and fluoroquinolones (FQ). In order to obtain new compounds with superior antibacterial and antifungal properties, we synthesized two silver complexes of NOR. A multi-analytical approach was carried out by using elemental analysis (C, H, N), conductivity measurement, differential scanning calorimetry and spectroscopic methods (FT-IR, H-NMR, UV-VIS and PL). The screening of antibacterial activity and antifungal of the complexes was performed by determination of minimal inhibitory concentration (MIC) through microtiter broth dilution method.
Even if serotyping based on O antigens is still routinely used by most laboratories for the detec... more Even if serotyping based on O antigens is still routinely used by most laboratories for the detection of diarrheagenic Escherichia coli, this method can provide false-positive reactions, due to the high diversity of O antigens. Molecular methods represent a valuable tool that clarifies these situations. In the Bacteriology Laboratory of Mureș County Hospital, between May 2016 and July 2019, 160 diarrheagenic E. coli strains were isolated from children under 2 years old with diarrheic disease. The strains were identified as Shiga toxin-producing E. coli (STEC)/enteropathogenic Escherichia coli (EPEC) via agglutination with polyvalent sera. STEC strains were serotyped using monovalent sera for serogroup O157. Simplex PCR was performed on the strains to determine the presence of the hlyA gene, and, for the positive ones, the hemolytic activity was tested. Antibiotic susceptibility of the identified diarrheagenic E. coli strains was also investigated. STEC strains were the most frequent...
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[Detection of the sensitivity of some bacteria and fungi to the action of sodium phosphotungstate beta-Na6[P2W18O62].18 H2O]
Poliyoxometalales are a very special category of the chemical compounds that have multiply proper... more Poliyoxometalales are a very special category of the chemical compounds that have multiply properties and possibility to application in a lot of actual domain of the scientific research. One of the most practice application of the polyoxometalates with a special significance is their antibacterial and anti fungus actions. For this study, there have been used fungus, Gram-positive and Gram-negative bacteria, and the action of the polyoxometalates was tested simultaneously with the action of the specifically antibiotics for the studied bacteria. In order to determine the fungus and bacteria sensibility to the tested substance, there have been used three methods: two qualitative, diffusion methods (rondel and bucket methods) and one quantitative method (determination of the minimum inhibitory concentration-MIC). The results of the bucket method were similarly with ones obtained with the rondel method: only Staphylococcus aureus strains were sensitive to the sodium phosphotungstate. Usi...
[Evaluation of iron, fluoride and iodine concentrations in underground water in some counties in Romania]
AIM To compare the underground water mineral composition in iron, fluorine and iodine from differ... more AIM To compare the underground water mineral composition in iron, fluorine and iodine from different sources, with variable locations in Romania. METHODS It is a lab study based on Hanna Instruments C99 colorimeter used for our tests, that follows the levels of mineral parameters from water samples from several underground water supplie sources from 10 different romanian counties. RESULTS It were significant differences between sources from different counties, and also from different sources in the same county, regarding minerals levels in water and also comparing to admitted levels. The most various data were related to fluorine and iodine levels in water samples and risk of exposure by deficiency. CONCLUSION It is important to know the importance of supply water monitoring, to evaluate both the drinking water quality parameters and mineral composition, in order to estimate the influence on health status of long shut consumer population.
The increase of antibiotic resistant bacteria in lasts years resulted in limited options for trea... more The increase of antibiotic resistant bacteria in lasts years resulted in limited options for treatment of bacterial diseases. Escherichia coli is one of the most common Gram-negative bacterial pathogen and a cause of both community and hospital acquired infections. Medicinal plants are alternative rich sources of useful antibacterial agents. The antimicrobial activities of the spruce (Picea abies L.) bark polyphenolic extracts were examined for their capacity to inhibit the growth of Escherichia coli. Spruce bark extract was obtained by conventional aqueous extraction and with ultrasounds. The minimum inhibitory concentration was determined by microdilution method. The antibacterial effect of both extracts was strong against Escherichia coli. The antimicrobial effect of polyphenolic extracts on Escherichia coli was expressed at a concentration of 15 mg/ml. Antimicrobial activity of spruce bark compounds suggest a possible use of spruce bark in pharmaceutical preparations.
The aim of the present study was to analyze the clo nal relations and virulence profile of clinic... more The aim of the present study was to analyze the clo nal relations and virulence profile of clinically r elevant MRSA strains collected during one year from pat ients admitted to clinical wards with high risk for nosocomial infections in a university hospital. One hundred and twenty two MRSA strains isolated from patients admitted to surgical and intensive care units during 2010 we re analyzed. Molecular characterization was perform ed using PFGE (pulsed-field gel electrophoresis), determinin g spa polymorphism and SCCmec type. The presence of genes encoding for virulence factors was assessed. We have identified 25 distinct pulsotypes clusterin g into 4 major clonal groups. Group A comprised 82% of MRSA strains (n=100), corresponding to spa type t030. Th ese strains harboured SCCmec type III and were positive for enterotoxin A genes. Fifteen percent of the st rains (n=18) belonged to group B representing spa types t 127, t015 and t321, carrying SCCmec type IV and gen es encoding for ...