Priti Tomar - Academia.edu (original) (raw)

Papers by Priti Tomar

Current Probiotics

: Humans serve as hosts for various species of microorganisms that contribute to the maintenance ... more : Humans serve as hosts for various species of microorganisms that contribute to the maintenance of human health. The beneficial bacteria residing in our bodies are referred to as probiotics. Besides improving the immune system, probiotics are also capable of warding off various diseases. In recent decades, probiotic microorganisms have emerged as a significant factor in human well-being. Popularly referred to as "health-friendly mi-croorganisms," they influence health in myriad ways by boosting the immune system, combating intestinal irritation, improving the balance of the gut microbiome, lowering blood pressure, relieving postmenopausal symptoms, and reducing traveler’s diarrhea. The ability of probiotics to compete for the adhesion sites of pathogenic microbes makes them an effective treatment against various intestinal pathogens. Recent studies also ad-vocate their use in the treatment of skin and oral diseases. In addition, the modulation of gut flora by probiotics is being discussed as a possible new solution for treating ten-sion and depression. Ultimately, a cost-effective approach is imperative to improve and maintain fitness in the present competitive environment. This review aims to evaluate the potential benefits of probiotic microorganisms in various disorders, such as gastrointesti-nal disorders, UTIs, acne, anticancer properties, gut-brain connections, and their possible mode of action.

Pharmaceutical Biology, 2012

The search for newer compounds against pathogenic species continues unabated due to drug resistan... more The search for newer compounds against pathogenic species continues unabated due to drug resistance. Traditionally, Tagetes erecta Linn. (Compositae) has been used for the treatment of various parasitic and microbial diseases. To evaluate the antioxidant activity of the ethanol extract of Tagetes erecta roots and its cytotoxicity against prostate and HeLa cancer cell lines followed by activity-guided isolation. The antioxidant screening was carried out using diphenylpicrylhydrazyl (DPPH) radical scavenging assay with serial concentrations ranging from 2 to 100 µg/mL, and cytotoxicity was evaluated against prostate (PC-3) and HeLa cell lines using microculture tetrazolium test (MTT) assay with concentrations ranging from 500 to 1.89 µg/mL. Isolation of the ethanol extract was carried out using column chromatography whereby 21 isolates were obtained (T₁-T₂₁), and the most active isolate was subjected for characterization using ultraviolet (UV), infrared (IR), nuclear magnetic resonance (NMR), and mass spectroscopic techniques. The ethanol extract scavenged DPPH free radicals thereby exhibiting antioxidant activity with an IC₅₀ of 35.9 µg/mL. In addition, the extract conferred noticeable cytotoxicity against the HeLa (LD₅₀ of 164.28 µg/mL) and PC-3 cell lines (LD₅₀ of 407.3 µg/mL). Among all the isolates, T₃ showed antioxidant activity with IC₅₀ of 11.56 µg/mL and cytotoxicity with LD₅₀ of 12.5 µg/mL against HeLa and 30.25 µg/mL against PC-3 cell lines and was characterized as 2-ethynyl-5-(thiophen-2-yl) thiophene. The new thienyl compound (T₃) exhibited profound antioxidant activity and cytotoxicity at relatively lower concentrations than the extract. The observations provide support for the ethnobotanical use of the plant.

Acta Biochimica et Biophysica Sinica, 2011

The purpose of the present work was to develop hepatitis B surface antigen (HBsAg) surface-adsorb... more The purpose of the present work was to develop hepatitis B surface antigen (HBsAg) surface-adsorbed cationic poly (D,L-lactic-co-glycolic acid) PLGA nanoparticles for interferon alpha (IFNa) delivery targeted to hepatocytes. Cationic PLGA nanoparticles loaded with IFNa were prepared using the double emulsification technique. Delipidated HBsAg was passively adsorbed on the surface of nanoparticles by using the simple dipping and drying method. Surface morphology and size distribution of nanoparticles were analyzed by scanning electron microscopy and dynamic light-scattering method, respectively. The biodistribution behavior of plain and HBsAgcoated 99m Tc-tagged PLGA nanoparticles was also examined followed by intravenous injection. The results revealed that ∼75% of the radioactivity was recovered in the liver after 4 h of injection that was nearly 3-fold greater in magnitude than the plain PLGA nanoparticles. These data demonstrated that the novel formulation of nanoparticles has potential application in hepatic-targeted drug delivery.

Drug Delivery and Translational Research, 2012

Goserelin acetate (Gos) is a synthetic analogue of luteinizing hormone-releasing hormone (LHRH) u... more Goserelin acetate (Gos) is a synthetic analogue of luteinizing hormone-releasing hormone (LHRH) used in treatment of prostate cancer. The objective of this study is to investigate the in vitro cytotoxic effect of Goserelin loaded nanoparticles on both androgen dependent and androgen independent cell lines. Goserelin causes cell death of prostate cancer cell lines by inducing apoptosis. Treatment of Goserelin loaded nanoparticles inhibited the proliferation of LNCaP and DU145 in the dose-dependent manner, however did not affect the cell proliferation in PC-3 cell lines. Blank nanoparticles exhibited negligible cytotoxicity on cell lines. In addition, immunocytochemical studies indicated that Gos induced apoptosis in prostate cancer cells. The presence and characteristics of LHRH receptors and their messenger ribonucleic acid (mRNA) expression in human prostate cancer cell line LNCaP were investigated by polymerase chain reaction. Changes in nuclear morphology and DNA fragmentation associated with Gos induced apoptosis were clearly seen in both LNCaP and DU145 cell lines by DNA studies. The PCR product of the expected size of 319 bp for human LHRH receptors was obtained in cell line sample. Goserelin loaded nanoparticles are the potential tool for site specific delivery for prostate cancer treatment.

Plant Growth Regulation, 2011

Oxytocin, a peptide animal hormone, was used as a growth regulator to test its effect on biomass ... more Oxytocin, a peptide animal hormone, was used as a growth regulator to test its effect on biomass accumulation and production of secondary plant constituent glycyrrhizin in the cell cultures of Abrus precatorius. Glycyrrhizin is an important phytoconstituent of liquorice which is widely used in the pharmaceutical and food industries. Cell suspension cultures of A. precatorius were developed from leaf explant of in vitro germinated plant in Murashige and Skoog medium supplemented with 30 g/l sucrose, 1 mg/l naphthalene acetic acid and 1 mg/l kinetin. The influence of oxytocin on biomass accumulation as well as on the production of glycyrrhizin was observed in the cell cultures of A. precatorius. Treatment of A. precatorius cell cultures with 100 lg/l oxytocin, improved glycyrrhizin production up to 34.27 mg/l on the dry cell weight basis third day after oxytocin treatment, which is over four times that of the control cultures, simultaneously nearly two fold increase in the biomass 2 days after the oxytocin treatment was recorded over the control cultures.

Pharmaceutical Development and Technology, 2010

The major disadvantage of several currently available vaccines is the need for repeated administr... more The major disadvantage of several currently available vaccines is the need for repeated administrations. The aim of the study was to develop long-acting microspheres based on poly(-ε-caprolactone) (PCL) for delivery of recombinant hepatitis B surface antigen (rHBsAg). PCL microspheres were prepared for induction of humoral and cellular immunity by intramuscular administration. Microspheres were characterized for their size, shape, incorporation efficiency, zeta potential, antigen integrity, antigen conformation and immunogenicity. DSC (Differential Scanning Calorimetry) studies revealed that better encapsulation efficiency between high and low mol wt polymer. The Circular Dichorism spectroscopy (CD) of antigen, released from PCL microspheres revealed that the secondary structure of antigen was unperturbed. Antigen integrity was evaluated by SDS-PAGE. Immunization with HBsAg PCL microspheres resulted in upregulation of specific cellular (IFN-γ and IL-2) as well as IgG response in BALB/c mice. Immune responses were found significantly higher than the conventional alum adjuvant following a single intramuscular immunization. These results highlight the enhanced efficiency of these PCL microspheres as an adjuvant and their prospective use in the prevention of hepatitis B.

In Vitro Cellular & Developmental Biology - Plant, 2010

... from seeds of A. precatorius was also used as an effective anticancer agent (Subbareddy and S... more ... from seeds of A. precatorius was also used as an effective anticancer agent (Subbareddy and Sirsi 1969; Ramnath et al ... R. Jain .P. Tomar .VK Dixit (*) Biotechnology Research Laboratory, Department of Pharmaceutical Sciences, Dr. HS Gour University, Sagar, Madhya Pradesh ...

Pharmacognosy magazine, 2011

Glycyrrhizin, obtained from Abrus precatorius (Indian liquorice), is a phytoconstituent of import... more Glycyrrhizin, obtained from Abrus precatorius (Indian liquorice), is a phytoconstituent of importance for pharmaceutical and food industries.

Pharmaceutical development and technology, 2012

Goserelin acetate (Gos) is a luteinizing hormone-releasing hormone agonist, used in treatment of ... more Goserelin acetate (Gos) is a luteinizing hormone-releasing hormone agonist, used in treatment of prostate cancer in which desired concentration of Gos in blood is maintained for longer duration. The aim of this study is to improve the efficacy of Gos targeted at the site of action and eliminate the need for frequent administration. Gos-encapsulated nanoparticles were fabricated by double emulsification process. The physicochemical traits of the nanoparticles including morphology, particle size, zeta-potential, entrapment efficiency, and in-vitro release profile were studied. The in-vitro cytotoxicity of the blank nanoparticles and Gos-loaded nanoparticles were also evaluated on LNCaP cell line by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Blank methoxy PEG-poly(ϵ-caprolactone) (mPEG-PCL) nanoparticles exhibited low cytotoxicity, which increased with increase in concentration of Gos-loaded nanoparticles. Serum Gos and testosterone levels were analyzed after s...

Pharmaceutical Development and Technology, 2011