Rukam S Tomar - Academia.edu (original) (raw)

Papers by Rukam S Tomar

Journal of Oilseeds Research

Down regulation of ahFAD2 results in reduced conversion of oleic to linoleic acid thus enhancing ... more Down regulation of ahFAD2 results in reduced conversion of oleic to linoleic acid thus enhancing O/L ratio ingroundnut. Novel gene editing technique, CRISPR-CAS9 was used to edit ahFAD2 gene in groundnut to enhance O/L ratio. Guide RNA (gRNA) was identified and designed targeting 1200 bp within the 1710 bp long ahFAD2 gene. Later, gRNA was successfully cloned into p53 construct having, CAS9. Entire construct was transferred into Agrobacterium and validated using PCR primers. Explants co-cultured with Agrobacterium, produced multiple shoots. Transformed shoots were selected on hygromycin selective media.

In the era of food nutritional security, the development of minerals-rich grains is an essence fo... more In the era of food nutritional security, the development of minerals-rich grains is an essence for fighting malnutrition. In the present study, we tried to identify the transcripts responsible for the higher accumulation of grain-Fe in Indian barnyard millet through transcriptome sequencing of genotype BAR-1433 (high Fe content) and BAR-1423 (low Fe content) during two stages of spike development i.e., spike emergence and milking stage. During the spike emergence stage, a set of 895 up-regulated and 126 down-regulated transcripts were identified between the high and low grain-Fe containing genotype, while during the milking stage, the number of up-regulated and down-regulated transcripts were 436 and 285. The transcripts which were commonly up-regulated during both the stages were having roles in nucleolar protein, metal-nicotianamine transporter, ribonucleoprotein complex, Vinorine synthase, Cellulose synthase, Auxin response factor, embryogenesis abundant protein, Cytochrome c oxi...

Background: Peanut (Arachis hypogaeaL.) is one of the valuable oilseed crops grown in drought-pro... more Background: Peanut (Arachis hypogaeaL.) is one of the valuable oilseed crops grown in drought-prone areas worldwide. Drought seriously limits peanut production and productivitysignificantly. Method and Results: In order to decipher the drought tolerance mechanism in peanut under drought stress, RNA sequencing was performed in TAG -24 (drought tolerant genotype) and JL-24 (drought susceptible genotype). Approximately 51 million raw reads were generated from four different libraries of two genotypes subjected to drought stress exerted by 20% PEG 6000 stress and control conditions, of which ~41 million (80.87%) filtered reads were mapped to the Arachis hypogaea L. reference genome. The transcriptome analysis detected 1,629 differentially expressed genes (DEGs), 186 genes encoding transcription factors (TFs) and 30,199 SSR among the identified DEGs. The comparative analysis between the two genotypes revealed higher and integral tolerance in TAG-24 through activation of key genes involve...

Parthenium hysterophorus L. is a notorious weed, which significantly reduce yield and quality of ... more Parthenium hysterophorus L. is a notorious weed, which significantly reduce yield and quality of crops and causes several problems to human health. In, present study an attempt was made to understand the economic value, and survival nature of Parthenium through deep transcriptome analysis. Transcriptome analysis of leaf and root tissue of P. hysterophorus had resulted 7,832,143 reads in case of leaves, and 9,646,830 reads in case of roots sample with longest read length of 300 and 298 nucleotides, respectively. A total of 35,719 contigs were produced with an average length of 548bp after an assembly in all two samples. The Blastn of the above generated contigs with 61,901 sequences of P. argentatum resulted in the identification of 25,947 novel contigs specific to P. hysterophorus. The Kyoto Encyclopedia of Genes and Genomes pathway based analysis showed the expression of genes associated with pathways pertaining to biosynthesis of Glucosinolate, Amino acids, and Aminobenzoate degra...

Fenugreek (Trigonella foenum-graecum L.) popularly known as “Methi” belongs to family fabaceae an... more Fenugreek (Trigonella foenum-graecum L.) popularly known as “Methi” belongs to family fabaceae and subfamily papilionaceae. Gujarat Methi Variety 2 was selected for transcriptome study from the three developmental stages that were vegetative stage (20-30 DAS), reproductive stage (50-60 DAS) and maturity stage (80-90 DAS) which were sequenced using Ion S5 genome sequencing machine. Differential gene expression analysis was carried out by using CLC genomic workbench (v10.0.0). In which the data generated from transcriptome sequencing of fenugreek were mapped with the reference genome Arabidopsis thaliana, the reference genome with a length of 119,667,750 bp, total number of genes present in the reference genome were 32,833 and 49,477 transcripts. In gene expression analysis from all the three developmental stages, top 10 highly expressed genes and 10 lower regulated genes were selected. In vegetative stage, the highest expression value was 474910 and RPKM was 73070.44 for highly expre...

International Journal of Current Microbiology and Applied Sciences, 2019

Электронный Научно Образовательный Вестник Здоровье И Образование В Xxi Веке, 2012

BMC Plant Biology, 2019

Background: Soybean (Glycine max L. Merril) crop is major source of edible oil and protein for hu... more Background: Soybean (Glycine max L. Merril) crop is major source of edible oil and protein for human and animals besides its various industrial uses including biofuels. Phytoplasma induced floral bud distortion syndrome (FBD), also known as witches' broom syndrome (WBS) has been one of the major biotic stresses adversely affecting its productivity. Transcriptomic approach can be used for knowledge discovery of this disease manifestation by morpho-physiological key pathways. Results: We report transcriptomic study using Illumina HiSeq NGS data of FBD in soybean, revealing 17,454 differentially expressed genes, 5561 transcription factors, 139 pathways and 176,029 genic region putative markers single sequence repeats, single nucleotide polymorphism and Insertion Deletion. Roles of PmbA, Zn-dependent protease, SAP family and auxin responsive system are described revealing mechanism of flower bud distortion having abnormalities in pollen, stigma development. Validation of 10 randomly selected genes was done by qPCR. Our findings describe the basic mechanism of FBD disease, right from sensing of phytoplasma infection by host plant triggering molecular signalling leading to mobilization of carbohydrate and protein, phyllody, abnormal pollen development, improved colonization of insect in host plants to spread the disease. Study reveals how phytoplasma hijacks metabolic machinery of soybean manifesting FBD. Conclusions: This is the first report of transcriptomic signature of FBD or WBS disease of soybean revealing morphological and metabolic changes which attracts insect for spread of disease. All the genic region putative markers may be used as genomic resource for variety improvement and new agro-chemical development for disease control to enhance soybean productivity.

The Pharma Innovation Journal, 2021

The fatty acid (FA) composition of soybean (Glycine max L. Merrill) oil determines its stability.... more The fatty acid (FA) composition of soybean (Glycine max L. Merrill) oil determines its stability. The FA composition of 40 Indian soybean genotype collected from

Background: Noncommunicable diseases have become a public heath problem in India concomitant with... more Background: Noncommunicable diseases have become a public heath problem in India concomitant with economic development, leading to increases in tobacco consumption, obesity, and changes in diet and lifestyle. Although observation suggests that tobacco consumption is a major risk factor for deaths due to circulatory, pulmonary, and malignant diseases, such studies are not available from most populations in developing countries. Subjects and methods: For the period 1999–2001, we studied the randomly selected records of death of 2222 (1385 men and 837 women) decedents, aged 25–64 years, out of 3034 death records overall from the records at Municipal Corporation, Moradabad. All the families of these deceased could be contacted individually to find out the causes of death, by scientist/doctor administered, informed consented, verbal autopsy questionnaire, completed with the help of the spouse and local treating doctor practicing in the appropriate healthcare region. Social classes and to...

Journal of Horticultural Science, 2016

The parent Hara Madhu in E1, AMM-01-18 and AMM-02 -26 in E2 and AMM-01-18, AMM-02-26 and Hara Mad... more The parent Hara Madhu in E1, AMM-01-18 and AMM-02 -26 in E2 and AMM-01-18, AMM-02-26 and Hara Madhu on pooled basis exhibited positive and significant GCA effects for fruit yield per plant. Thus these three parents appeared to be good general combiners for fruit yield. Out of these parents AMM-01-18 had a good combining ability for fruit yield per plant, number of primary branches, number of fruits per plant, fruit weight, moisture content, total soluble solids, acidity and total soluble sugars on pooled basis. Specific combining ability effects for fruit yield and yield attributing traits revealed significant and positive SCA effects in fourteen crosses for number of primary branches per plant, nine for fruit length, twelve for fruit girth, ten for fruits per plant, eleven for fruit weight, nine for fruit yield per plant, eleven for flesh thickness, nine for moisture content, twenty for total soluble solids, twenty for acidity and fifteen for total soluble sugars data in the desire...

Journal of Horticultural Sciences, 2016

Ten parental lines and 45 F1 hybrids of muskmelon obtained from half dialleles were studied to in... more Ten parental lines and 45 F1 hybrids of muskmelon obtained from half dialleles were studied to investigate the extent of heterosis in muskmelon. Heterotic effects over the better parent were observed to be higher for number of the node on which first female flower appeared, fruits per plant, fruit weight, fruit yield per plant, moisture content and total soluble sugars in E 2 than in E 1 . The hybrids AMM-01-18 x AMM-02-26, Hara Madhu x RM- 50, AMM-00-25 x AMM-00-11 and AMM-01-18 x DM-1 were found to be high-yielding and heterotic in both the seasons studied and even when averaged over the two environments, with other yield attributes and quality traits. Hence, after sufficient evaluation, these hybrids were identified as potential hybrids for widespread cultivation and commercial exploitation.

INTERNATIONAL JOURNAL OF AGRICULTURAL SCIENCES, 2020

International journal of chronic obstructive pulmonary disease, 2007

Noncommunicable diseases have become a public heath problem in India concomitant with economic de... more Noncommunicable diseases have become a public heath problem in India concomitant with economic development, leading to increases in tobacco consumption, obesity, and changes in diet and lifestyle. Although observation suggests that tobacco consumption is a major risk factor for deaths due to circulatory, pulmonary, and malignant diseases, such studies are not available from most populations in developing countries. For the period 1999-2001, we studied the randomly selected records of death of 2222 (1385 men and 837 women) decedents, aged 25-64 years, out of 3034 death records overall from the records at Municipal Corporation, Moradabad. All the families of these deceased could be contacted individually to find out the causes of death, by scientist/doctor administered, informed consented, verbal autopsy questionnaire, completed with the help of the spouse and local treating doctor practicing in the appropriate healthcare region. Social classes and tobacco intakes were assessed by a q...

Plant Breeding, 2015

A widely grown but rust susceptible Indian wheat variety HD2932 was improved for multiple rust re... more A widely grown but rust susceptible Indian wheat variety HD2932 was improved for multiple rust resistance by marker-assisted transfer of genes Lr19, Sr26 and Yr10. Foreground and background selection processes were practised to transfer targeted genes with the recovery of the genome of HD2932. The near-isogenic lines (NILs) of HD2932 carrying Lr19, Sr26 and Yr10 were individually produced from two backcrosses with recurrent parent HD2932. Marker-assisted background selection of NILs with 94.38-98.46% of the HD2932 genome facilitated rapid recovery of NILs carrying Lr19, Sr26 and Yr10. In the BC 2 F 2 generation, NILs were intercrossed and two gene combinations of Lr19+Yr10, Sr26 + Yr10 and Lr19+Sr26 were produced. A total of 16 progeny of two gene combinations of homozygous NILs of HD2932 have been produced, which are under seed increase for facilitating the replacement of the susceptible HD2932 with three of the sixteen improved backcross lines with resistance to multiple rusts.

Indian Journal of Microbiology, 2013

Enterobacter sp. MR1 an endophytic plant growth promoting bacterium was isolated from the roots o... more Enterobacter sp. MR1 an endophytic plant growth promoting bacterium was isolated from the roots of Butea monosperma, a drought tolerant plant. Genome sequencing of Enterobacter spp. MR1 was carried out in Ion Torrent (PGM), Next Generation Sequencer. The data obtained revealed 640 contigs with genome size of 4.58 Mb and G?C content of 52.8 %. This bacterium may contain genes responsible for inducing drought tolerance in plant, including genes for phosphate solubilization, growth hormones and other useful genes for plant growth.

Acta Cardiologica, 2007

Objective and design-The objective of the study was to find out the prevalence of overweight, obe... more Objective and design-The objective of the study was to find out the prevalence of overweight, obesity, undernutrition and physical activity status in the urban populations of India. Cross-sectional surveys were conducted in 6-12 urban streets in each of five cities in five different regions of India using a common study protocol and criteria of diagnosis. Subjects and methods-A total of 6940∞ ∞ subjects (3433 women and 3507∞ ∞ men) aged 25∞ ∞ years and above were randomly selected from the cities of Moradabad (n∞ ∞ =∞ ∞ 2002),Trivandrum (n∞ ∞ =∞ ∞ 1602), Calcutta (n∞ ∞ =∞ ∞ 900), Nagpur (n∞ ∞ =∞ ∞ 894) and Bombay (n∞ ∞ =∞ ∞ 1542). Evaluation and validation were performed by a physician and dietitian-administered questionnaire at Moradabad. After pooling of data, all subjects were divided into various age groups for men and women. Obesity (body mass index∞ ∞ =∞ ∞ 30 ≥ kg/m 2) and overweight (BMI ≥ 25-29.9∞ ∞ kg/m 2 and∞ ∞ >∞ ∞ 23∞ ∞ kg/m 2) as well as waist-hip ratio (∞ ∞ >∞ ∞ 0.85 in women and∞ ∞ >∞ ∞ 0.88 in men, central obesity) were calculated and physical activity status assessed by a validated questionnaire. Results-The overall prevalence of obesity was 6.8% (7.8 vs. 6.2%, P∞ ∞ <∞ ∞ 0.05) and overweight 33.5% (35.0 vs. 32.0%, P∞ ∞ <∞ ∞ 0.05) among women and men, respectively.The highest prevalence of obesity (7.8%) and overweight (36.9%) was found among subjects aged 35 to 44∞ ∞years in both sexes. The prevalence of obesity was significantly (P <∞ ∞0.05) greater in Trivandrum (8.5%), Calcutta (7.1%) and Bombay (8.3%) compared to Moradabad (6.2%) among women and in Trivandrum (7.4%) and Bombay (7.2%), compared to Nagpur (5.0%) among men.There was a significant decreasing trend in obesity (P <∞ ∞0.05) and overweight (P∞ ∞<∞ ∞0.05) with increasing age above 35-44∞ ∞years in both sexes.The overall prevalence of subjects∞ ∞>∞ ∞23∞ ∞kg/m 2 was 50.8% and central obesity 52.6%.The overall prevalence of sedentary behaviour was 59.3% among women and 58.5% among men. Both sedentary behaviour and mild activity showed a significant increasing trend in women after the age of 35-44∞ ∞ years. In men, such a trend was observed above the age of 45∞∞years. Sedentary behaviour was significantly (P <∞ ∞0.05) greater in Trivandrum, Calcutta, and Bombay compared to Nagpur. Sedentary behaviour was significantly (P <∞ ∞ 0.001) associated with obesity in both sexes, compared to non-obese men and women. The overall prevalence of undernutrition was 5.5% (n∞ ∞=∞ ∞380) which was significantly more common in Moradabad, north and Nagpur, central India compared to other cities. Conclusions-Obesity, overweight and central obesity and sedentary behaviour coexist with undernutrition, and have become a public health problem in all the five cities of India.The prevalence of obesity and sedentary behaviour was significantly greater in Trivandrum, Calcutta and Bombay compared to Moradabad and Nagpur. Sedentary behaviour was significantly associated with obesity compared to non-obese subjects in both sexes, which may be due to greater economic development in metro cities.