Tomasz Zatonski - Academia.edu (original) (raw)
Papers by Tomasz Zatonski
![Research paper thumbnail of [Prognostic value of CDC25 phosphatases expression in laryngeal cancer]](https://attachments.academia-assets.com/47181207/thumbnails/1.jpg)
](Otolaryngologia polska. The Polish otolaryngology
CDC25 phosphatases, significant positive regulators of the cell cycle play a pivotal role in cont... more CDC25 phosphatases, significant positive regulators of the cell cycle play a pivotal role in controlling cell proliferation during development and tumorigenesis. The prevalence and clinical implications of CDC25 immunoreactivity in laryngeal squamous cell cancer (LSCC) patients however, have not been elucidated. The object of the study was to assess the relationship between the expression levels of CDC25A, CDC25B and clinicopathological parameters and overall survival time of patients with LSCC. Tissue blocks from 46 patients treated surgically at our institution between 1992 and 2000 were available for this study. Immunohistochemistry using polyclonal antibodies against CDC25A and CDC25B was used to examine proteins expression. Ki-67 antigen expression was examined as a cell proliferation marker. Control group consisted of 21 samples of unchanged mucosa. CDC25A and CDC25B expression was observed in 96% (44/46) and 56.5% (26/46) of tumors; the mean labeling index was 73.9% and 36.5%...
J Electrocardiol, 2010
Tubercular infection is still one of the most serious health and social problems. It's been estim... more Tubercular infection is still one of the most serious health and social problems. It's been estimated that one-third of the population is infected with Koch's bacillus. More than 90% of overall morbidity of tuberculosis in Poland pose pulmonary tuberculosis, in the worldwide level the percentage reaches 80. The most popular causes that spread the disease are famine, malnutrition, homelessness, limited availability to medical care, alcohol abuse, drug addiction, ageing of the society and more intensive migration. Among the cases of extrapulmonary tuberculosis the most common were certified as tuberculous empyema. Head and neck tuberculosis is diagnosed rarely nowadays. Its symptoms aren’t pathognomonic and natural history of the disease is different from those described in medical books. It is essential to enclose laryngeal carcinosis in differential diagnosis. In 2002 two cases of laryngeal tuberculosis were diagnosed in the Department of Otolaryngology Head and Neck Surgery of Medical University of Wroclaw. Both patients had sustained hoarseness. Biopsy confirmed the diagnose of tuberculosis. Both patients underwent the tuberculostatic treatment. Videolaryngoscopic examination showed complete withdrawal of the infiltration in the larynx.
Annals of Agricultural and Environmental Medicine Aaem, Sep 20, 2012
Tobacco smoking is still one of the greatest, avoidable, singular causes of death. Although stude... more Tobacco smoking is still one of the greatest, avoidable, singular causes of death. Although students of medical faculties are expected to have solid knowledge about smoking hazards, a significant number of them still smoke. The aim of the study was to assess knowledge on tobacco dependence in a sample of students at the Medical University in Wroclaw. Between 2009-2011, non-compulsory lectures on the diagnosis and treatment of tobacco dependence were provided for 3(rd) to 6(th) year students of medicine at the Medical University in Wroclaw (170 students). The questionnaire contained 10 questions about smoking-related diseases and medicines used in tobacco dependence treatment. 21% of students smoked cigarettes and 79% were never smokers. 36% of the study group was exposed to passive smoking at the university. Nearly 80% of survey respondents agreed with the statement that cigarette smoking can lead to psychological addiction as strong as drug addiction, but more than 12% of the respondents perceived smoking just as a strong habit. Only 6 out of 10 surveyed students recognised tobacco dependence as an illness classified in an international classification of diseases and health problems (ICD-10). The correct amount of the chemical substances to be found in tobacco smoke was known by 67.1% of all surveyed students. The vast majority of the surveyed students indicated correctly 2 brands of nicotine replacement therapy, but none of them could name even one chemical and corresponding trade name of the pharmaceutical with central effect. The level of knowledge about the diagnosis and treatment of tobacco dependence among the students of the Medical Faculty in Wroclaw Medical University is low, and requires improvement through educational activities at both facultative and compulsory study level. Special attention should be paid to pharmaceutical treatment of the tobacco dependence syndrome.
Neuro Endocrinology Letters, 2010
Acromegaly is a rare disease, which symptoms are caused by excess secretion of a growth hormone (... more Acromegaly is a rare disease, which symptoms are caused by excess secretion of a growth hormone (GH) from the anterior pituitary benign tumor - adenoma. Authors present an evaluation of computed tomography (CT) and magnetic resonance (MR) images of temporal bone and paranasal sinuses of patients with acromegaly. 1. In all patients with acromegaly, morphological changes in paranasal sinuses were shown. They were mostly pronounced within the maxillary sinuses including the mucosa thickening up to 6 mm and encysted fluid occurrence. 2. CT of temporal bone did not reveal structural changes of internal and median ear. 3. There is a need for further studies on hearing impairment in patients with acromegaly.
Polski merkuriusz lekarski : organ Polskiego Towarzystwa Lekarskiego, 2015
Squamous cell carcinoma of the external auditory canal is a very rare and unusual malignancy, rep... more Squamous cell carcinoma of the external auditory canal is a very rare and unusual malignancy, representing less than 0.2% of all head and neck cancers. The authors present a case of 78-year-old patient with bilateral squamous cell carcinoma of the external auditory canal-cT4N0M0 G2, pT4N2bM0, initially treated as a chronic otitis external. The patient was qualifies for the one-step removal of cancer of the earlobe and the external auditory canal. Because of complications during the operation, the plan of treatment was changed. The patient is under oncological control for 13 months.
Srpski arhiv za celokupno lekarstvo, 2015
Lipomas are the most common benign mesenchymal tumors, which account for almost 50% of all soft-t... more Lipomas are the most common benign mesenchymal tumors, which account for almost 50% of all soft-tissue tumors. The case of a 75-year-old patient with a slow growing lesion of para- and retropharyngeal space was reported. The patient was suffering from progressive dysphagia, respiratory obstruction and sleep apnea. An external surgical approach is the treatment of choice. Etiology, differential diagnosis and therapy of head and neck lipomas has been discussed.
Neuro endocrinology letters, 2010
Acromegaly is a rare disease, which symptoms are caused by excess secretion of a growth hormone (... more Acromegaly is a rare disease, which symptoms are caused by excess secretion of a growth hormone (GH) from the anterior pituitary benign tumor - adenoma. Authors present an evaluation of computed tomography (CT) and magnetic resonance (MR) images of temporal bone and paranasal sinuses of patients with acromegaly. 1. In all patients with acromegaly, morphological changes in paranasal sinuses were shown. They were mostly pronounced within the maxillary sinuses including the mucosa thickening up to 6 mm and encysted fluid occurrence. 2. CT of temporal bone did not reveal structural changes of internal and median ear. 3. There is a need for further studies on hearing impairment in patients with acromegaly.
Otolaryngologia Polska, 2007
CDC25 phosphatases, significant positive regulators of the cell cycle play a pivotal role in cont... more CDC25 phosphatases, significant positive regulators of the cell cycle play a pivotal role in controlling cell proliferation during development and tumorigenesis. The prevalence and clinical implications of CDC25 immunoreactivity in laryngeal squamous cell cancer (tSCCl patients however, have not been elucidated. Aim. The object of the study was to assess the relationship between the expression levels of CDC25A, CDCzsB and clinicopathological parameters and overall survival time of patients with LSCC. Material and methods. Tissue blocks from 46 patients treated surgically at our institution between 1992 and 200o were available for this study. ImmunohistochemisĘ using polyclonal antibodies against CDCzsA and CDC2SB was used to examine proteins expression. Ki-67 antigen expression was examined as a cell proliferation markor.
Otolaryngologia Polska, 2014
Otolaryngologia Polska, 2010
The Lancet Infectious Diseases, 2004
European Archives of Oto-Rhino-Laryngology, 2003
Matrix metalloproteinases (MMPs) that have a proteolytic activity against the components of extra... more Matrix metalloproteinases (MMPs) that have a proteolytic activity against the components of extracellular matrix (ECM) play an important role in the invasive and metastatic spread of tumors. The role of MMPs and tissue inhibitors of MMPs (TIMPs) in laryngeal squamous cell carcinoma (LSCC) has not been elucidated sufficiently. The aim of the present study was to evaluate the correlation between the expression of collagenase-1 (MMP-1), collagenase-3 (MMP-13) and TIMP-1, as well as the clinicopathological features of LSCCs. The expression of collagenases and TIMP-1 was examined immunohistochemically in 50 cases of surgically obtained specimens of primary LSCCs. Analyses indicated that LSCC cells as well as stromal cells expressed MMP-1, MMP-13 and TIMP-1 immunostaining. Overexpression of TIMP-1 occurred more frequently in non-metastasizing cases (P =0.009). TIMP-1 and MMP-1 staining correlated significantly with the histologic type of LSCC. The keratinizing type of carcinomas exhibited higher TIMP-1 protein expression than the nonkeratinizing variety (P =0.01). TIMP-1 staining was associated with the grade of differentiation, since it was found predominantly in well and moderately differentiated carcinomas (P =0.04). The findings confirm that expression of analyzed MMPs and TIMP-1 is characteristic of LSCC and that these enzymes contribute to the progression of tumors. TIMP-1 upregulation might exhibit lower metastatic potential in LSCCs and is linked rather with an early stage of tumor progression. It seems also that TIMP-1 expression is dependent on the grade of differentiation.
Clinical Otolaryngology and Allied Sciences, 2004
The Bcl-2 family of proteins regulate one of the steps in an evolutionary conserved apoptotic pat... more The Bcl-2 family of proteins regulate one of the steps in an evolutionary conserved apoptotic pathway. The long splice variant of Bcl-X (Bcl-Xl) is a potent antagonist of apoptosis. The aim of the study was to evaluate the relation between the presence of immunohistochemically detectable Bcl-Xl protein in laryngeal squamous cell carcinomas (LSCCs) and clinicopathological data, as well as DNA ploidy status and proliferative activity. In 50 specimens of LSCC, Bcl-Xl protein expression was evaluated immunohistochemically. Proliferative activity (SG 2 M-phase index) and DNA ploidy were measured by¯ow cytometry. In our study, Bcl-Xl protein expression decreased with decreasing tumour differentiation (P 0.04). The majority of patients with Bcl-Xl protein immunoreactivity had no metastatic lymph node involvement (P 0.01). Other factors such as age, gender, primary tumour size (pT) and type of cancer (keratinizing/non-keratinizing) were not associated with Bcl-Xl protein level. There was no correlation between Bcl-Xl protein and SG 2 M-phase index or DNA ploidy status. Our ®ndings show that expression of Bcl-Xl protein is increased in a great fraction of laryngeal cancers. Further studies, however, are needed to clarify association between Bcl-Xl protein expression and clinical course of patients.
Acta Oto-laryngologica, 2004
Expression of c-myc oncoprotein in laryngeal squamous cell carcinoma. Acta Otolaryngol 2004; 124:... more Expression of c-myc oncoprotein in laryngeal squamous cell carcinoma. Acta Otolaryngol 2004; 124: 634 Á/637.
Acta Oto-laryngologica, 2008
Oncology Reports, 2004
Extensive molecular studies in development of the squamous cell carcinoma of larynx (SCCL) indica... more Extensive molecular studies in development of the squamous cell carcinoma of larynx (SCCL) indicated the involvement of a variety of genes including the MLH1. To search for possible mechanism leading to MLH1 silencing in SCCL we studied LOH and promoter methylation in a homogeneous set of 62 larynx cancers. Then we evaluated immunohistochemically the MLH1 expression for 51 tumor specimens. Further, the results were correlated with microsatellite instability and subsequently with the clinical course of the disease. LOH at the MLH1 locus and aberrant methylation of its promoter were found in 47.9 and in 22.6% of tumors, respectively. A decreased expression was observed in 27.5% of the cases. MSI analysis of tumor DNA showed a microsatellite stable phenotype in 59 cases (95.2%). From our study it can be concluded that: i) molecular alterations of MLH1 play an important role in SSCL development, ii) both LOH and aberrant methylation contribute to the MLH1 inactivation in SCCL and are associated with a less advanced stage of differentiation of larynx tumors, iii) MLH1 inactivation does not lead to MSI in larynx cancer and MSI may not contribute to the development of SCCL.
Otolaryngologia polska. The Polish otolaryngology
CDC25 phosphatases, significant positive regulators of the cell cycle play a pivotal role in cont... more CDC25 phosphatases, significant positive regulators of the cell cycle play a pivotal role in controlling cell proliferation during development and tumorigenesis. The prevalence and clinical implications of CDC25 immunoreactivity in laryngeal squamous cell cancer (LSCC) patients however, have not been elucidated. The object of the study was to assess the relationship between the expression levels of CDC25A, CDC25B and clinicopathological parameters and overall survival time of patients with LSCC. Tissue blocks from 46 patients treated surgically at our institution between 1992 and 2000 were available for this study. Immunohistochemistry using polyclonal antibodies against CDC25A and CDC25B was used to examine proteins expression. Ki-67 antigen expression was examined as a cell proliferation marker. Control group consisted of 21 samples of unchanged mucosa. CDC25A and CDC25B expression was observed in 96% (44/46) and 56.5% (26/46) of tumors; the mean labeling index was 73.9% and 36.5%...
J Electrocardiol, 2010
Tubercular infection is still one of the most serious health and social problems. It's been estim... more Tubercular infection is still one of the most serious health and social problems. It's been estimated that one-third of the population is infected with Koch's bacillus. More than 90% of overall morbidity of tuberculosis in Poland pose pulmonary tuberculosis, in the worldwide level the percentage reaches 80. The most popular causes that spread the disease are famine, malnutrition, homelessness, limited availability to medical care, alcohol abuse, drug addiction, ageing of the society and more intensive migration. Among the cases of extrapulmonary tuberculosis the most common were certified as tuberculous empyema. Head and neck tuberculosis is diagnosed rarely nowadays. Its symptoms aren’t pathognomonic and natural history of the disease is different from those described in medical books. It is essential to enclose laryngeal carcinosis in differential diagnosis. In 2002 two cases of laryngeal tuberculosis were diagnosed in the Department of Otolaryngology Head and Neck Surgery of Medical University of Wroclaw. Both patients had sustained hoarseness. Biopsy confirmed the diagnose of tuberculosis. Both patients underwent the tuberculostatic treatment. Videolaryngoscopic examination showed complete withdrawal of the infiltration in the larynx.
Annals of Agricultural and Environmental Medicine Aaem, Sep 20, 2012
Tobacco smoking is still one of the greatest, avoidable, singular causes of death. Although stude... more Tobacco smoking is still one of the greatest, avoidable, singular causes of death. Although students of medical faculties are expected to have solid knowledge about smoking hazards, a significant number of them still smoke. The aim of the study was to assess knowledge on tobacco dependence in a sample of students at the Medical University in Wroclaw. Between 2009-2011, non-compulsory lectures on the diagnosis and treatment of tobacco dependence were provided for 3(rd) to 6(th) year students of medicine at the Medical University in Wroclaw (170 students). The questionnaire contained 10 questions about smoking-related diseases and medicines used in tobacco dependence treatment. 21% of students smoked cigarettes and 79% were never smokers. 36% of the study group was exposed to passive smoking at the university. Nearly 80% of survey respondents agreed with the statement that cigarette smoking can lead to psychological addiction as strong as drug addiction, but more than 12% of the respondents perceived smoking just as a strong habit. Only 6 out of 10 surveyed students recognised tobacco dependence as an illness classified in an international classification of diseases and health problems (ICD-10). The correct amount of the chemical substances to be found in tobacco smoke was known by 67.1% of all surveyed students. The vast majority of the surveyed students indicated correctly 2 brands of nicotine replacement therapy, but none of them could name even one chemical and corresponding trade name of the pharmaceutical with central effect. The level of knowledge about the diagnosis and treatment of tobacco dependence among the students of the Medical Faculty in Wroclaw Medical University is low, and requires improvement through educational activities at both facultative and compulsory study level. Special attention should be paid to pharmaceutical treatment of the tobacco dependence syndrome.
Neuro Endocrinology Letters, 2010
Acromegaly is a rare disease, which symptoms are caused by excess secretion of a growth hormone (... more Acromegaly is a rare disease, which symptoms are caused by excess secretion of a growth hormone (GH) from the anterior pituitary benign tumor - adenoma. Authors present an evaluation of computed tomography (CT) and magnetic resonance (MR) images of temporal bone and paranasal sinuses of patients with acromegaly. 1. In all patients with acromegaly, morphological changes in paranasal sinuses were shown. They were mostly pronounced within the maxillary sinuses including the mucosa thickening up to 6 mm and encysted fluid occurrence. 2. CT of temporal bone did not reveal structural changes of internal and median ear. 3. There is a need for further studies on hearing impairment in patients with acromegaly.
Polski merkuriusz lekarski : organ Polskiego Towarzystwa Lekarskiego, 2015
Squamous cell carcinoma of the external auditory canal is a very rare and unusual malignancy, rep... more Squamous cell carcinoma of the external auditory canal is a very rare and unusual malignancy, representing less than 0.2% of all head and neck cancers. The authors present a case of 78-year-old patient with bilateral squamous cell carcinoma of the external auditory canal-cT4N0M0 G2, pT4N2bM0, initially treated as a chronic otitis external. The patient was qualifies for the one-step removal of cancer of the earlobe and the external auditory canal. Because of complications during the operation, the plan of treatment was changed. The patient is under oncological control for 13 months.
Srpski arhiv za celokupno lekarstvo, 2015
Lipomas are the most common benign mesenchymal tumors, which account for almost 50% of all soft-t... more Lipomas are the most common benign mesenchymal tumors, which account for almost 50% of all soft-tissue tumors. The case of a 75-year-old patient with a slow growing lesion of para- and retropharyngeal space was reported. The patient was suffering from progressive dysphagia, respiratory obstruction and sleep apnea. An external surgical approach is the treatment of choice. Etiology, differential diagnosis and therapy of head and neck lipomas has been discussed.
Neuro endocrinology letters, 2010
Acromegaly is a rare disease, which symptoms are caused by excess secretion of a growth hormone (... more Acromegaly is a rare disease, which symptoms are caused by excess secretion of a growth hormone (GH) from the anterior pituitary benign tumor - adenoma. Authors present an evaluation of computed tomography (CT) and magnetic resonance (MR) images of temporal bone and paranasal sinuses of patients with acromegaly. 1. In all patients with acromegaly, morphological changes in paranasal sinuses were shown. They were mostly pronounced within the maxillary sinuses including the mucosa thickening up to 6 mm and encysted fluid occurrence. 2. CT of temporal bone did not reveal structural changes of internal and median ear. 3. There is a need for further studies on hearing impairment in patients with acromegaly.
Otolaryngologia Polska, 2007
CDC25 phosphatases, significant positive regulators of the cell cycle play a pivotal role in cont... more CDC25 phosphatases, significant positive regulators of the cell cycle play a pivotal role in controlling cell proliferation during development and tumorigenesis. The prevalence and clinical implications of CDC25 immunoreactivity in laryngeal squamous cell cancer (tSCCl patients however, have not been elucidated. Aim. The object of the study was to assess the relationship between the expression levels of CDC25A, CDCzsB and clinicopathological parameters and overall survival time of patients with LSCC. Material and methods. Tissue blocks from 46 patients treated surgically at our institution between 1992 and 200o were available for this study. ImmunohistochemisĘ using polyclonal antibodies against CDCzsA and CDC2SB was used to examine proteins expression. Ki-67 antigen expression was examined as a cell proliferation markor.
Otolaryngologia Polska, 2014
Otolaryngologia Polska, 2010
The Lancet Infectious Diseases, 2004
European Archives of Oto-Rhino-Laryngology, 2003
Matrix metalloproteinases (MMPs) that have a proteolytic activity against the components of extra... more Matrix metalloproteinases (MMPs) that have a proteolytic activity against the components of extracellular matrix (ECM) play an important role in the invasive and metastatic spread of tumors. The role of MMPs and tissue inhibitors of MMPs (TIMPs) in laryngeal squamous cell carcinoma (LSCC) has not been elucidated sufficiently. The aim of the present study was to evaluate the correlation between the expression of collagenase-1 (MMP-1), collagenase-3 (MMP-13) and TIMP-1, as well as the clinicopathological features of LSCCs. The expression of collagenases and TIMP-1 was examined immunohistochemically in 50 cases of surgically obtained specimens of primary LSCCs. Analyses indicated that LSCC cells as well as stromal cells expressed MMP-1, MMP-13 and TIMP-1 immunostaining. Overexpression of TIMP-1 occurred more frequently in non-metastasizing cases (P =0.009). TIMP-1 and MMP-1 staining correlated significantly with the histologic type of LSCC. The keratinizing type of carcinomas exhibited higher TIMP-1 protein expression than the nonkeratinizing variety (P =0.01). TIMP-1 staining was associated with the grade of differentiation, since it was found predominantly in well and moderately differentiated carcinomas (P =0.04). The findings confirm that expression of analyzed MMPs and TIMP-1 is characteristic of LSCC and that these enzymes contribute to the progression of tumors. TIMP-1 upregulation might exhibit lower metastatic potential in LSCCs and is linked rather with an early stage of tumor progression. It seems also that TIMP-1 expression is dependent on the grade of differentiation.
Clinical Otolaryngology and Allied Sciences, 2004
The Bcl-2 family of proteins regulate one of the steps in an evolutionary conserved apoptotic pat... more The Bcl-2 family of proteins regulate one of the steps in an evolutionary conserved apoptotic pathway. The long splice variant of Bcl-X (Bcl-Xl) is a potent antagonist of apoptosis. The aim of the study was to evaluate the relation between the presence of immunohistochemically detectable Bcl-Xl protein in laryngeal squamous cell carcinomas (LSCCs) and clinicopathological data, as well as DNA ploidy status and proliferative activity. In 50 specimens of LSCC, Bcl-Xl protein expression was evaluated immunohistochemically. Proliferative activity (SG 2 M-phase index) and DNA ploidy were measured by¯ow cytometry. In our study, Bcl-Xl protein expression decreased with decreasing tumour differentiation (P 0.04). The majority of patients with Bcl-Xl protein immunoreactivity had no metastatic lymph node involvement (P 0.01). Other factors such as age, gender, primary tumour size (pT) and type of cancer (keratinizing/non-keratinizing) were not associated with Bcl-Xl protein level. There was no correlation between Bcl-Xl protein and SG 2 M-phase index or DNA ploidy status. Our ®ndings show that expression of Bcl-Xl protein is increased in a great fraction of laryngeal cancers. Further studies, however, are needed to clarify association between Bcl-Xl protein expression and clinical course of patients.
Acta Oto-laryngologica, 2004
Expression of c-myc oncoprotein in laryngeal squamous cell carcinoma. Acta Otolaryngol 2004; 124:... more Expression of c-myc oncoprotein in laryngeal squamous cell carcinoma. Acta Otolaryngol 2004; 124: 634 Á/637.
Acta Oto-laryngologica, 2008
Oncology Reports, 2004
Extensive molecular studies in development of the squamous cell carcinoma of larynx (SCCL) indica... more Extensive molecular studies in development of the squamous cell carcinoma of larynx (SCCL) indicated the involvement of a variety of genes including the MLH1. To search for possible mechanism leading to MLH1 silencing in SCCL we studied LOH and promoter methylation in a homogeneous set of 62 larynx cancers. Then we evaluated immunohistochemically the MLH1 expression for 51 tumor specimens. Further, the results were correlated with microsatellite instability and subsequently with the clinical course of the disease. LOH at the MLH1 locus and aberrant methylation of its promoter were found in 47.9 and in 22.6% of tumors, respectively. A decreased expression was observed in 27.5% of the cases. MSI analysis of tumor DNA showed a microsatellite stable phenotype in 59 cases (95.2%). From our study it can be concluded that: i) molecular alterations of MLH1 play an important role in SSCL development, ii) both LOH and aberrant methylation contribute to the MLH1 inactivation in SCCL and are associated with a less advanced stage of differentiation of larynx tumors, iii) MLH1 inactivation does not lead to MSI in larynx cancer and MSI may not contribute to the development of SCCL.