Tung Hoang - Academia.edu (original) (raw)

Papers by Tung Hoang

Research paper thumbnail of Effect of light intensities on maturation and spawning of ablated Penaeus merguiensis female Penaeus merguiensis

The effect of three levels of light intensity (BRIGHT: 1100 lux; DIM: 2 lux; and DARK: 0 lux) on ... more The effect of three levels of light intensity (BRIGHT: 1100 lux; DIM: 2 lux; and DARK: 0 lux) on ovarian maturation and spawning of Penaeus merguiensis was investigated. The proportion of spawners, latency period, spawning rate, fecundity, spawning interval and number of days spent at stage IV per spawn were not significantly different ( P > 0.05) among the three light intensity treatments. Compared to dim light, bright light did not adversely affect the maturation and reproductive performance of ablated prawns. However, the percentage of spawning at stage III (31.6%) and mortality (26.7%) were significantly higher in the DARK treatment ( P < 0.05). A trend of increasing fecundity with an increase in light intensity was observed. Also, the hypothesis of whether the prawn eye is the only light receptor involved in ovarian maturation and spawning was tested by covering the remaining eye of ablated prawns with prepared caps made from black heatshrinkable tubing (polyolefin). These prawns were held under 1100 lux (BRI-COV treatment) and 2 lux (DIM-COV) with the DARK treatment as the control group. The proportion of spawners was lowest in the BRI-COV treatment and highest in the DARK treatment ( P < 0.05), whereas there was no significant difference between these two treatments and the DIM-COV treatment. The DARK treatment, however, had a significantly higher spawning rate ( P < 0.05). The significantly higher percentage of spawning at stage III and less time spent at stage IV per spawn of prawns in the DARK treatment ( P < 0.05) compared with the DIM-COV and BRI-COV treatments suggest that the prawn 0044-8486/02/$ -see front matter D 2002 Elsevier Science B.V. All rights reserved. PII: S 0 0 4 4 -8 4 8 6 ( 0 1 ) 0 0 8 1 4 -6 Aquaculture 209 (2002) 347 -358 eye may not be the only light receptor involving in ovarian maturation and spawning in P. merguiensis. D

Research paper thumbnail of Intensive Care Helps Business Stands in the Flash Flood of Shrimp Diseases

The shrimp farming industry in Vietnam has been heavily affected by AHPNS and WSD since late 2010... more The shrimp farming industry in Vietnam has been heavily affected by AHPNS and WSD since late 2010. Estimated by the country's Directorate of Fisheries, the economical loss was about US$1.6 and US$1.05 billion for 2011 and 2012, respectively. While researchers are trying to identify the causative agents of AHPNS, proactive aquaculturists are developing practical measures to conquer the disease outbreak. In general biosecurity is strengthened via careful selection of postlarvae for stocking, application of nowater-exchange models, using underground water and control of Vibrio by reduction of salinity or using probiotics. Furthermore, farmers are advised to keep pond bottom clean and provide plenty of dissolved oxygen right after stocking and throughout the crop. Feeding, preferably by automated feeders, should be managed carefully to avoid uncessary built-ups of organic wastes in pond. These tactics appear to work, reflected through witnessed successes in different infected areas. However, they are only possible with well-invested farms. The authors of this article consider that shrimp farmers should either go back to truly extensive model or intensify their existing systems. When virulent diseases are at the doors, intensive care is a must for all steps of the farming protocol. Surely, such a care is possible only if the size of the farming system allows. The specific model described below and its concepts have been practiced successfully in Vietnam, and could be considered by stakeholders as an active effort to strengthen the shrimp farming industry.

Research paper thumbnail of Rearing the spotted seahorse Hippocampus kuda by feeding live and frozen copepods collected from shrimp ponds

Aquaculture Research

This study evaluated the use of live and frozen copepods collected from shrimp ponds for rearing ... more This study evaluated the use of live and frozen copepods collected from shrimp ponds for rearing juveniles of the spotted seahorse Hippocampus kuda. Protein and HUFA contents in frozen copepods were all higher than in Artemia nauplii, the conventional live food for seahorse juveniles. The results of this study showed that copepods can be used as feed for rearing seahorse fry and juvenile. The spotted seahorse showed obvious preference for live copepods and rarely fed on dead copepods on the tank bottom. Furthermore, the combination of frozen copepods and live Artemia nauplii resulted in highest growth and highest survival of the experimental seahorses. Further research on possible effects of DHA:EPA ratio on survival and growth of young H. kuda is recommended.

Research paper thumbnail of Sản Xuất Giống Cá Biển Chất Lượng Cao Bằng Mương Nổi Đặt Trong Ao

Research paper thumbnail of ĐÁNH GIÁ KHẢ NĂNG TẠO FLOC VÀ XỬ LÝ AMMONIA CỦA VI KHUẨN Pseudomonas spp. PHÂN LẬP TỪ AO NUÔI TÔM CÔNG NGHIỆP

Công nghệ biofloc là giải pháp tiên tiến cho nghề nuôi tôm trên toàn thế giới. Nhờ việc bổ sung c... more Công nghệ biofloc là giải pháp tiên tiến cho nghề nuôi tôm trên toàn thế giới. Nhờ việc bổ sung cơ chất carbon vào hệ thống nuôi với tỉ lệ C/N được duy trì ở mức thích hợp, giúp vi khuẩn dị dưỡng phát triển vừa xử lý chất thải trong môi trường nuôi vừa tạo nguồn thức ăn bổ sung cho đối tượng nuôi. Nghiên cứu này phân lập Pseudomonas spp. từ hệ thống biofloc, đánh giá khả năng tạo floc và xử lý ammonia của Pseudomonas pseudoalcaligenes và Pseudomonas stutzeri trong 3 môi trường nuôi cấy khác nhau (DD1, DD2 và DD3). Các chủng được chọn để kiểm tra khả năng tạo floc thông qua hoạt tính lắng cho kết quả không khác nhau ý nghĩa (>53%). Kết quả cho thấy 2 chủng P. pseudoalcaligenes xử lý ammonia tốt hơn P. stutzeri (84% so với 54,7% sau 6 giờ) (P<0,05). Ngoài ra, trong môi trường DD3 cả 3 chủng vi khuẩn đều xử lý ammonia tốt hơn so với môi trường DD1 và DD2 (P<0,05). Các kết quả của nghiên cứu này cung cấp thêm thông tin hữu ích cho việc sử dụng Pseudomonas pseudoalcaligenes và Pseudomonas stutzeri làm probiotic trong hệ thống nuôi tôm theo công nghệ biofloc.

Research paper thumbnail of Proceedings of Biofloc Technology and Shrimp Diseases Workshop

Research paper thumbnail of A POTENTIAL LOW COST MEDIUM FOR CULTIVATION OF Dunaliella salina DCCBC15 IN VIETNAM

Dunaliella salina is a marine alga with high potential for beta-carotene (pro-vitamin A), up to m... more Dunaliella salina is a marine alga with high potential for beta-carotene (pro-vitamin A), up to more than 10% of cellular dry weight under appropriate stress conditions of limiting one of the following nutrients: nitrogen, phosphate, high light or high salt. Obtaining high biomass in low cost medium is the essential step of the two-phase culture system for beta-carotene production. For future development of beta-carotene production from Dunaliella in Vietnam, it is our first step to study a low cost medium using natural seawater enriched with industrial fertilizer N-P-K (30-15-10) commercially available in Vietnam. The primary results of biomass, cell density, chlorophyll a as well as growth rate are very promising to use this medium for mass culture of Dunaliella salina. The medium is recommended for growing other marine algae as well.

Research paper thumbnail of Effects of photoperiod and light intensity on growth, size variation, survival and cannibalism rate of barramundi (Lates calcarifer Bloch) from 15 to 33 mm total length

độ chiếu áng 1.480 lux (TL:32,48±1,33 mm, SGR L :3,64±0,20 mm, BW: 0,56±0,07 g and SGR W : 11,11±... more độ chiếu áng 1.480 lux (TL:32,48±1,33 mm, SGR L :3,64±0,20 mm, BW: 0,56±0,07 g and SGR W : 11,11±0,63%) (P<0,05). Chu kỳ quang không ảnh hưởng đến sinh trưởng, mức độ phân đàn, tỉ lệ sống và tỉ lệ ăn thịt đồng loại (P>0,05). Cá chết chủ yếu là do hiện tượng ăn thịt đồng loại (từ 12 đến 31%). Chỉ có một phần nhỏ (từ 0,44 đến 2,96%) là do chết không rõ nguyên nhân..

Research paper thumbnail of A low complexity, low power, programmable QRS detector based on wavelet transform for Implantable Pacemaker IC

2006 IEEE International SOC Conference, 2006

ABSTRACT This paper presents a QRS detector operating in multi-mode with low complexity, power sa... more ABSTRACT This paper presents a QRS detector operating in multi-mode with low complexity, power saving consumption for Implantable Pacemaker IC. The complexity of detector is optimized after testing dynamic range of internal signals. Correspondingly, static power ...

Research paper thumbnail of Effect of temperature on spawning of Penaeus merguiensis

Journal of Thermal Biology, 2002

The maturation rate and spawning performance of ablated Penaeus merguiensis were significantly (P... more The maturation rate and spawning performance of ablated Penaeus merguiensis were significantly (Po0:05) higher at 271C than at 231C. Increasing the temperature from 231C to 271C further improved spawning performance (Po0:05), whereas reducing the temperature from 271C to 231C impaired spawning. The effect of temperature during the inducing period was more critical than during the holding period. Prawn growth (% of weight gain), however, was not different between 231C and 271C over the 60-day experimental period (P > 0:05). r

Research paper thumbnail of Phase I Trial of Bevacizumab followed by Concurrent Radiation, Bevacizumab and Cisplatin for Locoregionally Advanced Squamous Cell Carcinoma of the Head and Neck

International Journal of Radiation Oncology*Biology*Physics, 2010

Research paper thumbnail of The effect of dislocation loops on the light emission of silicon LEDs

IEEE Electron Device Letters, 2000

Recently, different and apparently contradicting results were published regarding the influence o... more Recently, different and apparently contradicting results were published regarding the influence of crystal defects on the light emission efficiency of silicon LEDs at room temperature . In this paper we report our results on light emission of silicon p + n diodes with various defect engineering approaches. The p + region was formed either by ion implantation or by diffusion; and optionally, additional lattice damage was created by silicon ion implantation. The experiments clearly indicate that lattice defects have a detrimental effect on light emission, contrary to the results published in recent years.

Research paper thumbnail of Radiation hardened ULSI technology and design of 1M SRAM

Cell Transplantation, 1994

A radiation hardened 0.5 μm Ultra Large Scale Integration (ULSI) technology was successfully deve... more A radiation hardened 0.5 μm Ultra Large Scale Integration (ULSI) technology was successfully developed to support the full scale production of a 1 Megabit Static Random Access Memory (1M SRAM). This ULSI technology represents the latest in a family of epitaxial bulk silicon CMOS used to produce 64 K SRAM, 256 K SRAM, an enhanced 256 K SRAM, and finally

Research paper thumbnail of Spawning behaviour of Penaeus (Fenneropenaeus) merguiensis de Man and the effect of light intensity on spawning

Aquaculture Research, 2002

An experiment was conducted to investigate the effect of light intensity just prior to and during... more An experiment was conducted to investigate the effect of light intensity just prior to and during spawning of Penaeus (Fenneropenaeus) merguiensis de Man in the laboratory. Neither the presence of light nor light intensity (0, 10 and 1100 lux) had a significant effect on the spawning process or hatching rate in P. merguiensis. Difference in the percentage of spawners, percentage of incomplete spawning, fecundity and hatching rate were all insignificant (P . 0.05) among the three light treatments. However, spawning was delayed (up to 21 min on average) under 1100 lux with a high percentage of incomplete spawning, compared with the Control (0 lux). For the first time, spawning behaviour of P. merguiensis was observed and fully described. The spawning process can be divided into four phases: dormant, pre-spawning, spawning and post-spawning by distinct behaviour of prawn broodstock. The dormant phase was characterized by a quiescent and non-feeding period, whereas movement of prawn broodstock was active during the pre-spawning and post-spawning phases. Depending on the individual, spawning was completed within 3±5 min. Prawns often stayed still on the water surface during spawning with occasional manoeuvres to change the position. After a short rest at the termination of the spawning phase, prawn broodstock re-exhibited active movement as in the pre-spawning phase, but showing conspicuous care to the deposited eggs. The role of light in controlling ovarian development and spawning, and the behaviour of prawn broodstock in relation to maximizing fertilization rate are discussed.

Research paper thumbnail of Improved reproductive readiness of pond-reared broodstock Penaeus merguiensis by environmental manipulation

Aquaculture, 2003

Two environmental manipulation protocols were tested against a current maturation technique (CONT... more Two environmental manipulation protocols were tested against a current maturation technique (CONTROL) in an 81-day experiment. PROTOCOL 1 mimicked changes in the natural environment from winter to spring when ovarian maturation and spawning in Penaeus merguiensis starts. PROTOCOL 2 evaluated whether a clear improvement of temperature from a fluctuation period to a stable one could help trigger ovarian maturation. In phase A (first 30 days) of the experiment, temperature and day length were gradually increased from 22 to 27 jC and from 10 to 14 h, respectively, while light intensity was stepwise reduced from 360 to 240, 120 and 5 lx for PROTOCOL 1. For PROTOCOL 2, light intensity and day length were manipulated as for PROTOCOL 1, but temperature was cycled from 22 to 27 jC every 3 days. In phase B (the following 30 days) temperature, light intensity and day length were stably maintained at optimal levels (27 jC, 5 lx and 14 h light, respectively). During phase B, the proportion of female prawns reaching stages III and IV was significantly ( P<0.05) highest in PROTOCOL 1 and lowest in the CONTROL. Of the total spawns by unablated females recorded in this phase, 55.6% was in PROTOCOL 1, 33.3% in PROTOCOL 2 and only 11.1% in the CONTROL. Mass spawning was induced by unilateral eyestalk ablation on day 60. Spawning performance of prawns were monitored for a further 21-day period (phase C). Overall, PROTOCOL 1, PROTOCOL 2 and the CONTROL produced 2.36, 1.96 and 1.53 million eggs, respectively. The latency period of spawners was shortest ( P<0.05), whereas the proportion of spawners, fecundity, spawning rate and egg production/female were all improved in PROTOCOL 1. An improvement of prawn growth was 0044-8486/03/$ -see front matter D Aquaculture 221 (2003) 523 -534 observed in PROTOCOL 2. This study suggested that environmental manipulation could improve ovarian maturation and spawning rate of pond-reared P. merguiensis. D

Research paper thumbnail of Ovarian maturation of the banana prawn, Penaeus merguiensis de Man under different light intensities

Aquaculture, 2002

The effect of light intensity on ovarian maturation and spawning of unablated female Penaeus merg... more The effect of light intensity on ovarian maturation and spawning of unablated female Penaeus merguiensis was investigated in a 4 week experiment. The experiment had two different light intensity treatments: BRIGHT (1100 lx) and DIM (2 lx). At starting time, there were four groups of broodstock with four different ovarian maturation stages (II, III, IV and V) in each treatment. Results showed that light intensity plays an important role in controlling ovarian maturation of prawns, especially at the later stages (stages III and IV). Bright light showed an inhibitory effect, whereas dim light favoured ovarian maturation and spawning. Ovarian regression occurred in most stage III and stage IV prawns kept under bright light, while prawns at the same stages developed their ovaries further and spawned under dim light. Proportion of spawners (12.5%), mean number of spawns per prawn (0.12) and mean fecundity (87,300) of prawns were significantly lower ( P < 0.05) in the BRIGHT treatment, compared to the DIM treatment. Under light intensity of 2 lx, 41.7% of prawns spawned, producing 0.5 spawn per prawn on average with a mean fecundity of 117,300. The results also suggest that the sensitivity of broodstock to light increased from stages II to IV of ovarian maturation, while there were some evidence of light adaptation in stage V broodstock. This study confirmed multiple spawning of unablated P. merguiensis for the first time. Rematuration period was short (6 -7 days). D

Research paper thumbnail of Effect of light intensity on maturation and spawning of ablated female Penaeus merguiensis

Aquaculture, 2002

The effect of three levels of light intensity (BRIGHT: 1100 lux; DIM: 2 lux; and DARK: 0 lux) on ... more The effect of three levels of light intensity (BRIGHT: 1100 lux; DIM: 2 lux; and DARK: 0 lux) on ovarian maturation and spawning of Penaeus merguiensis was investigated. The proportion of spawners, latency period, spawning rate, fecundity, spawning interval and number of days spent at stage IV per spawn were not significantly different ( P > 0.05) among the three light intensity treatments. Compared to dim light, bright light did not adversely affect the maturation and reproductive performance of ablated prawns. However, the percentage of spawning at stage III (31.6%) and mortality (26.7%) were significantly higher in the DARK treatment ( P < 0.05). A trend of increasing fecundity with an increase in light intensity was observed. Also, the hypothesis of whether the prawn eye is the only light receptor involved in ovarian maturation and spawning was tested by covering the remaining eye of ablated prawns with prepared caps made from black heatshrinkable tubing (polyolefin). These prawns were held under 1100 lux (BRI-COV treatment) and 2 lux (DIM-COV) with the DARK treatment as the control group. The proportion of spawners was lowest in the BRI-COV treatment and highest in the DARK treatment ( P < 0.05), whereas there was no significant difference between these two treatments and the DIM-COV treatment. The DARK treatment, however, had a significantly higher spawning rate ( P < 0.05). The significantly higher percentage of spawning at stage III and less time spent at stage IV per spawn of prawns in the DARK treatment ( P < 0.05) compared with the DIM-COV and BRI-COV treatments suggest that the prawn 0044-8486/02/$ -see front matter D 2002 Elsevier Science B.V. All rights reserved. PII: S 0 0 4 4 -8 4 8 6 ( 0 1 ) 0 0 8 1 4 -6 Aquaculture 209 (2002) 347 -358 eye may not be the only light receptor involving in ovarian maturation and spawning in P. merguiensis. D

Research paper thumbnail of Influences of light intensity and photoperiod on moulting and growth of Penaeus merguiensis cultured under laboratory conditions

Aquaculture, 2003

The effects of light intensity (I, 750 and 75 lx) and photoperiod ( P, 12L/12D and 7L/5D) on moul... more The effects of light intensity (I, 750 and 75 lx) and photoperiod ( P, 12L/12D and 7L/5D) on moulting and growth of Penaeus merguiensis were investigated over 12 weeks under laboratory conditions. Both light intensity and photoperiod significantly ( P < 0.05) influenced the percentage weight gain (PWG), but did not influence moult frequency or moult interval. Mean PWG of prawns cultured at 750 lx was 198.4%, significantly ( P < 0.01) higher than those cultured at 75 lx (168.1%). Imposing two light/dark cycles (7L/5D) in 24 h resulted in a significantly higher growth of prawns ( P < 0.05) than the normal photoperiod (12L/12D). Prawns cultured at 750 lx had a lower food conversion ratio (FCR, 1.485) than those cultured at 75 lx (1.590, P < 0.05). The effect of light intensity on prawn growth was found to be stronger and more immediate than that of photoperiod. There was no interaction between light intensity and photoperiod. Although not significantly different ( P>0.05), prawns cultured at 750 lx tended to moult more frequently. Sexual dimorphism was not observed within the range of tested prawns. D

Research paper thumbnail of Single prokaryotic cell isolation and total transcript amplification protocol for transcriptomic analysis

Nature Protocols, 2015

Until recently, transcriptome analyses of single cells have been confined to eukaryotes. The info... more Until recently, transcriptome analyses of single cells have been confined to eukaryotes. The information obtained from single-cell transcripts can provide detailed insight into spatiotemporal gene expression, and it could be even more valuable if expanded to prokaryotic cells. Transcriptome analysis of single prokaryotic cells is a recently developed and powerful tool. Here we describe a procedure that allows amplification of the total transcript of a single prokaryotic cell for in-depth analysis. This is performed by using a laser-capture microdissection instrument for single-cell isolation, followed by reverse transcription via Moloney murine leukemia virus, degradation of chromosomal DNA with McrBC and DpnI restriction enzymes, single-stranded cDNA (ss-cDNA) ligation using T4 polynucleotide kinase and CircLigase, and polymerization of ss-cDNA to double-stranded cDNA (ds-cDNA) by Φ29 polymerase. This procedure takes ∼5 d, and sufficient amounts of ds-cDNA can be obtained from single-cell RNA template for further microarray analysis.

Research paper thumbnail of FlexDEF: Development framework for processor architecture implementation and evaluation

2011 7th Conference on Ph.D. Research in Microelectronics and Electronics, 2011

... At present, the tools comprising FlexTools are written in Haskell, presenting some challenges... more ... At present, the tools comprising FlexTools are written in Haskell, presenting some challenges and shortcomings. ... REFERENCES [1] M. Själander, P. Larsson-Edefors, and M. Björk, “A Flexible Datapath Interconnect for Embedded Applications,” in IEEE Computer Society Annual ...

Research paper thumbnail of Effect of light intensities on maturation and spawning of ablated Penaeus merguiensis female Penaeus merguiensis

The effect of three levels of light intensity (BRIGHT: 1100 lux; DIM: 2 lux; and DARK: 0 lux) on ... more The effect of three levels of light intensity (BRIGHT: 1100 lux; DIM: 2 lux; and DARK: 0 lux) on ovarian maturation and spawning of Penaeus merguiensis was investigated. The proportion of spawners, latency period, spawning rate, fecundity, spawning interval and number of days spent at stage IV per spawn were not significantly different ( P > 0.05) among the three light intensity treatments. Compared to dim light, bright light did not adversely affect the maturation and reproductive performance of ablated prawns. However, the percentage of spawning at stage III (31.6%) and mortality (26.7%) were significantly higher in the DARK treatment ( P < 0.05). A trend of increasing fecundity with an increase in light intensity was observed. Also, the hypothesis of whether the prawn eye is the only light receptor involved in ovarian maturation and spawning was tested by covering the remaining eye of ablated prawns with prepared caps made from black heatshrinkable tubing (polyolefin). These prawns were held under 1100 lux (BRI-COV treatment) and 2 lux (DIM-COV) with the DARK treatment as the control group. The proportion of spawners was lowest in the BRI-COV treatment and highest in the DARK treatment ( P < 0.05), whereas there was no significant difference between these two treatments and the DIM-COV treatment. The DARK treatment, however, had a significantly higher spawning rate ( P < 0.05). The significantly higher percentage of spawning at stage III and less time spent at stage IV per spawn of prawns in the DARK treatment ( P < 0.05) compared with the DIM-COV and BRI-COV treatments suggest that the prawn 0044-8486/02/$ -see front matter D 2002 Elsevier Science B.V. All rights reserved. PII: S 0 0 4 4 -8 4 8 6 ( 0 1 ) 0 0 8 1 4 -6 Aquaculture 209 (2002) 347 -358 eye may not be the only light receptor involving in ovarian maturation and spawning in P. merguiensis. D

Research paper thumbnail of Intensive Care Helps Business Stands in the Flash Flood of Shrimp Diseases

The shrimp farming industry in Vietnam has been heavily affected by AHPNS and WSD since late 2010... more The shrimp farming industry in Vietnam has been heavily affected by AHPNS and WSD since late 2010. Estimated by the country's Directorate of Fisheries, the economical loss was about US$1.6 and US$1.05 billion for 2011 and 2012, respectively. While researchers are trying to identify the causative agents of AHPNS, proactive aquaculturists are developing practical measures to conquer the disease outbreak. In general biosecurity is strengthened via careful selection of postlarvae for stocking, application of nowater-exchange models, using underground water and control of Vibrio by reduction of salinity or using probiotics. Furthermore, farmers are advised to keep pond bottom clean and provide plenty of dissolved oxygen right after stocking and throughout the crop. Feeding, preferably by automated feeders, should be managed carefully to avoid uncessary built-ups of organic wastes in pond. These tactics appear to work, reflected through witnessed successes in different infected areas. However, they are only possible with well-invested farms. The authors of this article consider that shrimp farmers should either go back to truly extensive model or intensify their existing systems. When virulent diseases are at the doors, intensive care is a must for all steps of the farming protocol. Surely, such a care is possible only if the size of the farming system allows. The specific model described below and its concepts have been practiced successfully in Vietnam, and could be considered by stakeholders as an active effort to strengthen the shrimp farming industry.

Research paper thumbnail of Rearing the spotted seahorse Hippocampus kuda by feeding live and frozen copepods collected from shrimp ponds

Aquaculture Research

This study evaluated the use of live and frozen copepods collected from shrimp ponds for rearing ... more This study evaluated the use of live and frozen copepods collected from shrimp ponds for rearing juveniles of the spotted seahorse Hippocampus kuda. Protein and HUFA contents in frozen copepods were all higher than in Artemia nauplii, the conventional live food for seahorse juveniles. The results of this study showed that copepods can be used as feed for rearing seahorse fry and juvenile. The spotted seahorse showed obvious preference for live copepods and rarely fed on dead copepods on the tank bottom. Furthermore, the combination of frozen copepods and live Artemia nauplii resulted in highest growth and highest survival of the experimental seahorses. Further research on possible effects of DHA:EPA ratio on survival and growth of young H. kuda is recommended.

Research paper thumbnail of Sản Xuất Giống Cá Biển Chất Lượng Cao Bằng Mương Nổi Đặt Trong Ao

Research paper thumbnail of ĐÁNH GIÁ KHẢ NĂNG TẠO FLOC VÀ XỬ LÝ AMMONIA CỦA VI KHUẨN Pseudomonas spp. PHÂN LẬP TỪ AO NUÔI TÔM CÔNG NGHIỆP

Công nghệ biofloc là giải pháp tiên tiến cho nghề nuôi tôm trên toàn thế giới. Nhờ việc bổ sung c... more Công nghệ biofloc là giải pháp tiên tiến cho nghề nuôi tôm trên toàn thế giới. Nhờ việc bổ sung cơ chất carbon vào hệ thống nuôi với tỉ lệ C/N được duy trì ở mức thích hợp, giúp vi khuẩn dị dưỡng phát triển vừa xử lý chất thải trong môi trường nuôi vừa tạo nguồn thức ăn bổ sung cho đối tượng nuôi. Nghiên cứu này phân lập Pseudomonas spp. từ hệ thống biofloc, đánh giá khả năng tạo floc và xử lý ammonia của Pseudomonas pseudoalcaligenes và Pseudomonas stutzeri trong 3 môi trường nuôi cấy khác nhau (DD1, DD2 và DD3). Các chủng được chọn để kiểm tra khả năng tạo floc thông qua hoạt tính lắng cho kết quả không khác nhau ý nghĩa (>53%). Kết quả cho thấy 2 chủng P. pseudoalcaligenes xử lý ammonia tốt hơn P. stutzeri (84% so với 54,7% sau 6 giờ) (P<0,05). Ngoài ra, trong môi trường DD3 cả 3 chủng vi khuẩn đều xử lý ammonia tốt hơn so với môi trường DD1 và DD2 (P<0,05). Các kết quả của nghiên cứu này cung cấp thêm thông tin hữu ích cho việc sử dụng Pseudomonas pseudoalcaligenes và Pseudomonas stutzeri làm probiotic trong hệ thống nuôi tôm theo công nghệ biofloc.

Research paper thumbnail of Proceedings of Biofloc Technology and Shrimp Diseases Workshop

Research paper thumbnail of A POTENTIAL LOW COST MEDIUM FOR CULTIVATION OF Dunaliella salina DCCBC15 IN VIETNAM

Dunaliella salina is a marine alga with high potential for beta-carotene (pro-vitamin A), up to m... more Dunaliella salina is a marine alga with high potential for beta-carotene (pro-vitamin A), up to more than 10% of cellular dry weight under appropriate stress conditions of limiting one of the following nutrients: nitrogen, phosphate, high light or high salt. Obtaining high biomass in low cost medium is the essential step of the two-phase culture system for beta-carotene production. For future development of beta-carotene production from Dunaliella in Vietnam, it is our first step to study a low cost medium using natural seawater enriched with industrial fertilizer N-P-K (30-15-10) commercially available in Vietnam. The primary results of biomass, cell density, chlorophyll a as well as growth rate are very promising to use this medium for mass culture of Dunaliella salina. The medium is recommended for growing other marine algae as well.

Research paper thumbnail of Effects of photoperiod and light intensity on growth, size variation, survival and cannibalism rate of barramundi (Lates calcarifer Bloch) from 15 to 33 mm total length

độ chiếu áng 1.480 lux (TL:32,48±1,33 mm, SGR L :3,64±0,20 mm, BW: 0,56±0,07 g and SGR W : 11,11±... more độ chiếu áng 1.480 lux (TL:32,48±1,33 mm, SGR L :3,64±0,20 mm, BW: 0,56±0,07 g and SGR W : 11,11±0,63%) (P<0,05). Chu kỳ quang không ảnh hưởng đến sinh trưởng, mức độ phân đàn, tỉ lệ sống và tỉ lệ ăn thịt đồng loại (P>0,05). Cá chết chủ yếu là do hiện tượng ăn thịt đồng loại (từ 12 đến 31%). Chỉ có một phần nhỏ (từ 0,44 đến 2,96%) là do chết không rõ nguyên nhân..

Research paper thumbnail of A low complexity, low power, programmable QRS detector based on wavelet transform for Implantable Pacemaker IC

2006 IEEE International SOC Conference, 2006

ABSTRACT This paper presents a QRS detector operating in multi-mode with low complexity, power sa... more ABSTRACT This paper presents a QRS detector operating in multi-mode with low complexity, power saving consumption for Implantable Pacemaker IC. The complexity of detector is optimized after testing dynamic range of internal signals. Correspondingly, static power ...

Research paper thumbnail of Effect of temperature on spawning of Penaeus merguiensis

Journal of Thermal Biology, 2002

The maturation rate and spawning performance of ablated Penaeus merguiensis were significantly (P... more The maturation rate and spawning performance of ablated Penaeus merguiensis were significantly (Po0:05) higher at 271C than at 231C. Increasing the temperature from 231C to 271C further improved spawning performance (Po0:05), whereas reducing the temperature from 271C to 231C impaired spawning. The effect of temperature during the inducing period was more critical than during the holding period. Prawn growth (% of weight gain), however, was not different between 231C and 271C over the 60-day experimental period (P > 0:05). r

Research paper thumbnail of Phase I Trial of Bevacizumab followed by Concurrent Radiation, Bevacizumab and Cisplatin for Locoregionally Advanced Squamous Cell Carcinoma of the Head and Neck

International Journal of Radiation Oncology*Biology*Physics, 2010

Research paper thumbnail of The effect of dislocation loops on the light emission of silicon LEDs

IEEE Electron Device Letters, 2000

Recently, different and apparently contradicting results were published regarding the influence o... more Recently, different and apparently contradicting results were published regarding the influence of crystal defects on the light emission efficiency of silicon LEDs at room temperature . In this paper we report our results on light emission of silicon p + n diodes with various defect engineering approaches. The p + region was formed either by ion implantation or by diffusion; and optionally, additional lattice damage was created by silicon ion implantation. The experiments clearly indicate that lattice defects have a detrimental effect on light emission, contrary to the results published in recent years.

Research paper thumbnail of Radiation hardened ULSI technology and design of 1M SRAM

Cell Transplantation, 1994

A radiation hardened 0.5 μm Ultra Large Scale Integration (ULSI) technology was successfully deve... more A radiation hardened 0.5 μm Ultra Large Scale Integration (ULSI) technology was successfully developed to support the full scale production of a 1 Megabit Static Random Access Memory (1M SRAM). This ULSI technology represents the latest in a family of epitaxial bulk silicon CMOS used to produce 64 K SRAM, 256 K SRAM, an enhanced 256 K SRAM, and finally

Research paper thumbnail of Spawning behaviour of Penaeus (Fenneropenaeus) merguiensis de Man and the effect of light intensity on spawning

Aquaculture Research, 2002

An experiment was conducted to investigate the effect of light intensity just prior to and during... more An experiment was conducted to investigate the effect of light intensity just prior to and during spawning of Penaeus (Fenneropenaeus) merguiensis de Man in the laboratory. Neither the presence of light nor light intensity (0, 10 and 1100 lux) had a significant effect on the spawning process or hatching rate in P. merguiensis. Difference in the percentage of spawners, percentage of incomplete spawning, fecundity and hatching rate were all insignificant (P . 0.05) among the three light treatments. However, spawning was delayed (up to 21 min on average) under 1100 lux with a high percentage of incomplete spawning, compared with the Control (0 lux). For the first time, spawning behaviour of P. merguiensis was observed and fully described. The spawning process can be divided into four phases: dormant, pre-spawning, spawning and post-spawning by distinct behaviour of prawn broodstock. The dormant phase was characterized by a quiescent and non-feeding period, whereas movement of prawn broodstock was active during the pre-spawning and post-spawning phases. Depending on the individual, spawning was completed within 3±5 min. Prawns often stayed still on the water surface during spawning with occasional manoeuvres to change the position. After a short rest at the termination of the spawning phase, prawn broodstock re-exhibited active movement as in the pre-spawning phase, but showing conspicuous care to the deposited eggs. The role of light in controlling ovarian development and spawning, and the behaviour of prawn broodstock in relation to maximizing fertilization rate are discussed.

Research paper thumbnail of Improved reproductive readiness of pond-reared broodstock Penaeus merguiensis by environmental manipulation

Aquaculture, 2003

Two environmental manipulation protocols were tested against a current maturation technique (CONT... more Two environmental manipulation protocols were tested against a current maturation technique (CONTROL) in an 81-day experiment. PROTOCOL 1 mimicked changes in the natural environment from winter to spring when ovarian maturation and spawning in Penaeus merguiensis starts. PROTOCOL 2 evaluated whether a clear improvement of temperature from a fluctuation period to a stable one could help trigger ovarian maturation. In phase A (first 30 days) of the experiment, temperature and day length were gradually increased from 22 to 27 jC and from 10 to 14 h, respectively, while light intensity was stepwise reduced from 360 to 240, 120 and 5 lx for PROTOCOL 1. For PROTOCOL 2, light intensity and day length were manipulated as for PROTOCOL 1, but temperature was cycled from 22 to 27 jC every 3 days. In phase B (the following 30 days) temperature, light intensity and day length were stably maintained at optimal levels (27 jC, 5 lx and 14 h light, respectively). During phase B, the proportion of female prawns reaching stages III and IV was significantly ( P<0.05) highest in PROTOCOL 1 and lowest in the CONTROL. Of the total spawns by unablated females recorded in this phase, 55.6% was in PROTOCOL 1, 33.3% in PROTOCOL 2 and only 11.1% in the CONTROL. Mass spawning was induced by unilateral eyestalk ablation on day 60. Spawning performance of prawns were monitored for a further 21-day period (phase C). Overall, PROTOCOL 1, PROTOCOL 2 and the CONTROL produced 2.36, 1.96 and 1.53 million eggs, respectively. The latency period of spawners was shortest ( P<0.05), whereas the proportion of spawners, fecundity, spawning rate and egg production/female were all improved in PROTOCOL 1. An improvement of prawn growth was 0044-8486/03/$ -see front matter D Aquaculture 221 (2003) 523 -534 observed in PROTOCOL 2. This study suggested that environmental manipulation could improve ovarian maturation and spawning rate of pond-reared P. merguiensis. D

Research paper thumbnail of Ovarian maturation of the banana prawn, Penaeus merguiensis de Man under different light intensities

Aquaculture, 2002

The effect of light intensity on ovarian maturation and spawning of unablated female Penaeus merg... more The effect of light intensity on ovarian maturation and spawning of unablated female Penaeus merguiensis was investigated in a 4 week experiment. The experiment had two different light intensity treatments: BRIGHT (1100 lx) and DIM (2 lx). At starting time, there were four groups of broodstock with four different ovarian maturation stages (II, III, IV and V) in each treatment. Results showed that light intensity plays an important role in controlling ovarian maturation of prawns, especially at the later stages (stages III and IV). Bright light showed an inhibitory effect, whereas dim light favoured ovarian maturation and spawning. Ovarian regression occurred in most stage III and stage IV prawns kept under bright light, while prawns at the same stages developed their ovaries further and spawned under dim light. Proportion of spawners (12.5%), mean number of spawns per prawn (0.12) and mean fecundity (87,300) of prawns were significantly lower ( P < 0.05) in the BRIGHT treatment, compared to the DIM treatment. Under light intensity of 2 lx, 41.7% of prawns spawned, producing 0.5 spawn per prawn on average with a mean fecundity of 117,300. The results also suggest that the sensitivity of broodstock to light increased from stages II to IV of ovarian maturation, while there were some evidence of light adaptation in stage V broodstock. This study confirmed multiple spawning of unablated P. merguiensis for the first time. Rematuration period was short (6 -7 days). D

Research paper thumbnail of Effect of light intensity on maturation and spawning of ablated female Penaeus merguiensis

Aquaculture, 2002

The effect of three levels of light intensity (BRIGHT: 1100 lux; DIM: 2 lux; and DARK: 0 lux) on ... more The effect of three levels of light intensity (BRIGHT: 1100 lux; DIM: 2 lux; and DARK: 0 lux) on ovarian maturation and spawning of Penaeus merguiensis was investigated. The proportion of spawners, latency period, spawning rate, fecundity, spawning interval and number of days spent at stage IV per spawn were not significantly different ( P > 0.05) among the three light intensity treatments. Compared to dim light, bright light did not adversely affect the maturation and reproductive performance of ablated prawns. However, the percentage of spawning at stage III (31.6%) and mortality (26.7%) were significantly higher in the DARK treatment ( P < 0.05). A trend of increasing fecundity with an increase in light intensity was observed. Also, the hypothesis of whether the prawn eye is the only light receptor involved in ovarian maturation and spawning was tested by covering the remaining eye of ablated prawns with prepared caps made from black heatshrinkable tubing (polyolefin). These prawns were held under 1100 lux (BRI-COV treatment) and 2 lux (DIM-COV) with the DARK treatment as the control group. The proportion of spawners was lowest in the BRI-COV treatment and highest in the DARK treatment ( P < 0.05), whereas there was no significant difference between these two treatments and the DIM-COV treatment. The DARK treatment, however, had a significantly higher spawning rate ( P < 0.05). The significantly higher percentage of spawning at stage III and less time spent at stage IV per spawn of prawns in the DARK treatment ( P < 0.05) compared with the DIM-COV and BRI-COV treatments suggest that the prawn 0044-8486/02/$ -see front matter D 2002 Elsevier Science B.V. All rights reserved. PII: S 0 0 4 4 -8 4 8 6 ( 0 1 ) 0 0 8 1 4 -6 Aquaculture 209 (2002) 347 -358 eye may not be the only light receptor involving in ovarian maturation and spawning in P. merguiensis. D

Research paper thumbnail of Influences of light intensity and photoperiod on moulting and growth of Penaeus merguiensis cultured under laboratory conditions

Aquaculture, 2003

The effects of light intensity (I, 750 and 75 lx) and photoperiod ( P, 12L/12D and 7L/5D) on moul... more The effects of light intensity (I, 750 and 75 lx) and photoperiod ( P, 12L/12D and 7L/5D) on moulting and growth of Penaeus merguiensis were investigated over 12 weeks under laboratory conditions. Both light intensity and photoperiod significantly ( P < 0.05) influenced the percentage weight gain (PWG), but did not influence moult frequency or moult interval. Mean PWG of prawns cultured at 750 lx was 198.4%, significantly ( P < 0.01) higher than those cultured at 75 lx (168.1%). Imposing two light/dark cycles (7L/5D) in 24 h resulted in a significantly higher growth of prawns ( P < 0.05) than the normal photoperiod (12L/12D). Prawns cultured at 750 lx had a lower food conversion ratio (FCR, 1.485) than those cultured at 75 lx (1.590, P < 0.05). The effect of light intensity on prawn growth was found to be stronger and more immediate than that of photoperiod. There was no interaction between light intensity and photoperiod. Although not significantly different ( P>0.05), prawns cultured at 750 lx tended to moult more frequently. Sexual dimorphism was not observed within the range of tested prawns. D

Research paper thumbnail of Single prokaryotic cell isolation and total transcript amplification protocol for transcriptomic analysis

Nature Protocols, 2015

Until recently, transcriptome analyses of single cells have been confined to eukaryotes. The info... more Until recently, transcriptome analyses of single cells have been confined to eukaryotes. The information obtained from single-cell transcripts can provide detailed insight into spatiotemporal gene expression, and it could be even more valuable if expanded to prokaryotic cells. Transcriptome analysis of single prokaryotic cells is a recently developed and powerful tool. Here we describe a procedure that allows amplification of the total transcript of a single prokaryotic cell for in-depth analysis. This is performed by using a laser-capture microdissection instrument for single-cell isolation, followed by reverse transcription via Moloney murine leukemia virus, degradation of chromosomal DNA with McrBC and DpnI restriction enzymes, single-stranded cDNA (ss-cDNA) ligation using T4 polynucleotide kinase and CircLigase, and polymerization of ss-cDNA to double-stranded cDNA (ds-cDNA) by Φ29 polymerase. This procedure takes ∼5 d, and sufficient amounts of ds-cDNA can be obtained from single-cell RNA template for further microarray analysis.

Research paper thumbnail of FlexDEF: Development framework for processor architecture implementation and evaluation

2011 7th Conference on Ph.D. Research in Microelectronics and Electronics, 2011

... At present, the tools comprising FlexTools are written in Haskell, presenting some challenges... more ... At present, the tools comprising FlexTools are written in Haskell, presenting some challenges and shortcomings. ... REFERENCES [1] M. Själander, P. Larsson-Edefors, and M. Björk, “A Flexible Datapath Interconnect for Embedded Applications,” in IEEE Computer Society Annual ...