U. Schumacher - Academia.edu (original) (raw)

Papers by U. Schumacher

Research paper thumbnail of Fetuin in human bone marrow: detection in foetal tissue and patients with mastocytosis

The Histochemical journal, 2001

Fetuin, a foetal protein of unknown function, has been shown to be expressed in both the immune a... more Fetuin, a foetal protein of unknown function, has been shown to be expressed in both the immune and nervous systems, especially during development. Here, we show for the first time, that fetuin is abundantly present in many cells of the foetal human bone marrow, but is restricted to cells of the monocytic lineage in the adult. Fetuin's immunoreactivity increased considerably in adult human bone marrow in some pathological conditions, particularly in mastocytosis and was also increased in bone marrows in some cases of acute leukaemias, especially in acute myeloid leukaemia. This increase in the presence of fetuin in neoplastic bone marrows is not reflected by an increased level of circulating fetuin. This last observation contradicts earlier suggestions that fetuin is specifically reduced in cancer patients. A consistent increase in fetuin immunoreactivity in bone marrow of most cases of mastocytosis, as demonstrated in this paper, could become a useful tool in the diagnosis of t...

Research paper thumbnail of Exploring the visible human's inner organs with the VOXEL-MAN 3D navigator

Studies in health technology and informatics, 2001

Improved rendering and segmentation techniques lead to a new quality of 3D reconstructions of the... more Improved rendering and segmentation techniques lead to a new quality of 3D reconstructions of the Visible Human. Using these we have implemented an interactive atlas of anatomy and radiology of the inner organs.

Research paper thumbnail of Peritonitis in childhood: aspects of pathogenesis and therapy

Pediatric Surgery International, 2000

The pathogenesis of peritonitis due to hollowviscus perforation is currently accepted as being ma... more The pathogenesis of peritonitis due to hollowviscus perforation is currently accepted as being mainly based on the local and systemic release of pro-and anti-in¯ammatory mediators triggered by the presence of bacteria and bacterial products in the abdominal cavity. Therefore, treatment consists in focal restoration, intraoperative debridement and lavage, and postoperative measures such as drainage, continuous peritoneal lavage, or scheduled reoperation aiming at the removal of infectious agents from the peritoneal cavity to prevent persisting peritonitis and sepsis. In order to evaluate the pathophysiologic relevance of the bacterial and endotoxin load of the peritoneal exudate, we examined the peritoneal¯uid of 20 children with perforated appendicitis for qualitative and quantitative analysis of bacteria, antibiotic concentrations, and endotoxin content. The time period ranged in 12-h intervals from intraoperatively to day 5. Eighteen of 20¯uid specimens (90%) showed endotoxin levels above 1.5 endotoxin units EU/ml (standard <0.1 EU/ml). The most common bacterial species isolated was Escherichia coli, mostly in high concentrations. Despite persisting high endotoxin concentrations and bacterial loads in the peritoneal cavity during the 5 postoperative days, the children recovered uneventfully and the systemic signs of infection disap-peared rapidly. In conclusion, neither the bacterial nor the endotoxin load of the peritoneal cavity proved to be associated with the clinical course. Therefore, we hypothesize that during peritonitis compartmentalization of the focus of infection prevents further systemic reactions and ultimately leads to removal of the infectious agents by endogenous mechanisms. At least in peritonitis due to perforated appendicitis in children, adjuvant surgical measures in addition to appendectomy and intraoperative debridement are not necessary.

Research paper thumbnail of Lectin histochemistry of the gastric mucosa in normal and Helicobacter pylori infected guinea-pigs

Journal of Molecular Histology, 2005

Research paper thumbnail of Comparison of the unmasking of lectin receptors by neuraminidase and by enzyme-free buffer alone

Journal of Histochemistry & Cytochemistry, 1989

Research paper thumbnail of Inositol 1,4,5-Trisphosphate 3-Kinase-A Is a New Cell Motility-promoting Protein That Increases the Metastatic Potential of Tumor Cells by Two Functional Activities

Journal of Biological Chemistry, 2010

In growth factor-stimulated cells, the catalytically active domain enhances basal ITPKA-induced m... more In growth factor-stimulated cells, the catalytically active domain enhances basal ITPKA-induced migration by activating storeoperated calcium entry through production of inositol 1,3,4,5tetrakisphosphate and subsequent inhibition of inositol phosphate 5-phosphatase. These two functional activities of ITPKA stimulating tumor cell migration place the enzyme among the potential targets of anti-metastatic therapy. . The abbreviations used are: Ins(1,4,5)P 3 , inositol 1,4,5-trisphosphate; ITPKA, inositol 1,4,5-trisphosphate 3-kinase-A; HPLC, high pressure liquid chromatography; FCS, fetal calf serum; PBS, phosphate-buffered saline; EGF, epidermal growth factor; SOCE, store-operated Ca 2ϩ entry; CaM, calmodulin; GFP, green fluorescent protein; ITPK, 1,4,5-trisphosphate 3-kinase; GST, glutathione S-transferase; 5-PPT, inositol phosphate 5-phosphatase; IPMK, inositol polyphosphate multikinase; ABD, actin binding domain; Ins(1,3,4,5)P 4 , inositol-1,3,4,5-tetrakisphosphate; shRNA, short hairpin RNA; kd, knockdown.

Research paper thumbnail of Morphologische und laborchemische Untersuchungen an Wildkaninchen (Oryctolagus cuniculus (L.)) — eine vorläufige Bilanz zur Klärung der Frage nach den Ursachen des Besatzrückganges

Zeitschrift für Jagdwissenschaft, 1999

In den letzten Jahren hat sich der Kaninchenbesatz in vielen Regionen Deutschlands trotz anschein... more In den letzten Jahren hat sich der Kaninchenbesatz in vielen Regionen Deutschlands trotz anscheinend guter Habitatbedingungen so stark vermindert, daß keine Bejagung mehr lohnend ist. Im Gegensatz dazu gibt es Regionen, in denen dieser Rückgang nicht zu verzeichnen ist; eine Erklärung für diese lokalen und landesweiten Bestandseinbrüche steht aus.

Research paper thumbnail of Different myosins in myoid and entodermal reticular epithelial cells of the thymus

Cell and Tissue Research, 1978

A sensitive immunofluorescence microscopical technique employing specific antibodies against high... more A sensitive immunofluorescence microscopical technique employing specific antibodies against highly purified actin and (smooth muscle type) myosin from chicken gizzard and myosin from human striated muscle was used to localize these contractile proteins in the thymus of rat, guinea-pig, cat and chicken. Myoid cells were seen to react with antibodies to striated, but not to smooth muscle type myosin, whereas reticular epithelial cells contained smooth, but not striated type myosin. Actin immunoreactivity was found in both myoid and reticular epithelial cells.

Research paper thumbnail of cis Interaction of the Cell Adhesion Molecule CEACAM1 with Integrin β3

The American Journal of Pathology, 2001

CEACAM1 is a cell adhesion molecule that has been implicated in a number of physiological process... more CEACAM1 is a cell adhesion molecule that has been implicated in a number of physiological processes (eg, tumor suppressor in epithelial tissues, potent angiogenic factor in microvessel formation, microbial receptor in human granulocytes and epithelial cells). The mechanism of CEACAM1 action is still largely unresolved but recent findings demonstrated that the cytoplasmic CEACAM1 domain is linked indirectly to the actin-based cytoskeleton. We have isolated integrin ␤ 3 as an associated protein using CEACAM1 tail affinity purification. This association depends on phosphorylation of Tyr-488 in the CEACAM1 cytoplasmic domain. Confocal laser scanning microscopy confirmed in vivo colocalization of both molecules in human granulocytes and epithelial cells. Furthermore, the concentrated colocalization at the tumor-stroma interface of invading melanoma masses suggests a functional role of CEACAM1-integrin ␤ 3 interaction in melanoma invasion. Moreover, colocalization of the two adhesion molecules is also found at the apical surface of glandular cells of pregnancy endometrium. Colocalization of CEACAM1 and integrin ␤ 3 at the transitional zone from proliferative to invasive extravillous trophoblast of the maternalfetal interface supports a role for CEACAM1/integrin ␤ 3 complexes in cell invasion. (Am J Pathol 2001, 159:537-546) Supported by grant Br 1741/1-1 from the Deutsche Forschungsgemeinschaft to J. B. and C. W.

Research paper thumbnail of E-/P-selectins and colon carcinoma metastasis: first in vivo evidence for their crucial role in a clinically relevant model of spontaneous metastasis formation in the lung

British journal of cancer, Jan 2, 2010

Interactions of endothelial selectins with tumour cell glycoconjugates have been shown to have a ... more Interactions of endothelial selectins with tumour cell glycoconjugates have been shown to have a major role in tumour cell dissemination in previous experiments. However, experiments validating this observation were limited in value, as 'metastases' in these experiments were artificially induced by i.v. injection rather than developed spontaneously as in true metastases. Endothelial (E) and platelet (P)-selectin-deficient severe combined immunodeficient (scid) mice were generated and human HT 29 colon cancer cells were subcutaneously inoculated in these mice and in wild-type scid mice. Tumour growth, spontaneous metastasis formation in the lung and adherence of HT29 cells to E- and P-selectin under flow were determined. The number of metastases decreased by 84% in E- and P-selectin-deficient scid mice, compared with wild-type scid mice. The remaining 16% metastases in the E- and P-selectin-deficient scid mice grew within the pulmonary artery and not in the alveolar septae as...

Research paper thumbnail of Binding of mistletoe lectins to cutaneous malignant melanoma: implications for prognosis and therapy

Anticancer research

Glycoconjugates, as detected by lectin histochemistry, have been implicated in metastasis formati... more Glycoconjugates, as detected by lectin histochemistry, have been implicated in metastasis formation in many neoplasias. However, no data concerning the three mistletoe lectins (MLs) and the spread of malignant melanoma have been published. The binding status of ML-I, -II and -III was histochemically assessed in 100 malignant melanomas and correlated with metastasis in a 10 year follow-up period. Furthermore, the staining intensity of the three MLs, scored from negative (-) to very intense (+ + +), was evaluated. Kaplan-Meier analsis revealed that very intense binding (+ + +) of ML-I was positively-correlated with metastasis (p=0.044). Since ML-I is specific for galactose, high density galactose expression in malignant melanoma is a predictor of poor prognosis.

Research paper thumbnail of A realistic model of human structure from the visible human data

Methods of information in medicine, 2001

The computer-based 3D models of the human body reported to date suffer from poor spatial resoluti... more The computer-based 3D models of the human body reported to date suffer from poor spatial resolution. The Visible Human project has delivered high resolution cross-sectional images that are suited for generation of high-quality models. Yet none of the 3D models described to date reflect the quality of the original images. We present a method of segmentation and visualization which provides a new quality of realism and detail. Using the example of a 3D model of the inner organs, we demonstrate that such models, especially when combined with a knowledge base, open new possibilities for scientific, educational, and clinical work.

Research paper thumbnail of Structural, histochemical and biochemical observations on horse milk-fat-globule membranes and casein micelles

Histochemistry, 1988

Horse milk fat globules (MFGs) and casein micelles were studied using freeze fracturing, freeze e... more Horse milk fat globules (MFGs) and casein micelles were studied using freeze fracturing, freeze etching and thin-section electron microscopy, as well as lectin histochemistry, gel electrophoresis, and Western blotting. Horse MFGs were found to be relatively small, their average volume-surface diameter being about 2.75 microns. The MFG membrane is composed of three layers: an inner proteinaceous coat occasionally having a paracrystalline substructure, a unit membrane, and a prominent filamentous glycocalyx. The last is rich in glycoconjugates, as revealed by its binding of various lectins. In addition, the glycocalyx binds cationized ferritin, which indicates the presence of negative electric charges. Gel electrophoresis revealed the presence of high-molecular-weight glycoproteins in the MFG membrane of horse milk. Such glycoproteins are also present in human MFG membranes but are absent in the bovine MFGs. The casein micelles in horse milk are relatively large, their average volume-...

Research paper thumbnail of Histochemistry of mucosubstances in Brunner's-gland cells and duodenal goblet cells of two New World monkey species (Saimiri sciureus and Saguinus fuscicollis)

Histochemistry, 1988

Duodenal goblet cells and Brunner's-gland cells obtained from two species of New World monkey... more Duodenal goblet cells and Brunner's-gland cells obtained from two species of New World monkeys (Saimiri sciureus and Saguinus fuscicollis) were studied using conventional histochemical methods and by applying a panel of 17 labelled lectins. The secretions of both goblet and Brunner's-gland cells were found to contain neutral mucosubstances, while those of goblet cells also exhibit acid and sulphated carbohydrate components. Lectin binding studies allowed a more detailed analysis of the mucus glycoproteins. Marked differences between the two examined species were not detected. N-Acetyl-galactosamine, galactose, fucose and N-Acetyl-glucosamine were found to be the predominant sugar residues in Brunner's-glands glycoproteins, with mannose and glucose being only minor components.

Research paper thumbnail of Basic biochemical data on blood from antarctic Weddell seals (Leptonychotes weddelli): ions, lipids, enzymes, serum proteins and thyroid hormones

Comparative biochemistry and physiology. Comparative physiology, 1992

1. Standard laboratory values of blood samples taken from Weddell seals in Antarctica were determ... more 1. Standard laboratory values of blood samples taken from Weddell seals in Antarctica were determined. 2. Numerous blood parameters are similar to those observed in man. 3. Comparatively high cholesterol levels but normal triglyceride levels were observed when compared with humans. 4. In comparison to laboratory findings in humans, T4 levels were decreased although T3 levels were normal. 5. The levels of alkaline phosphatase are considerably higher than those in humans. 6. The data obtained indicate different lipid and thyroid hormone metabolism in Weddell seals when compared with humans.

Research paper thumbnail of Histochemical similarities of mucins produced by Brunner's glands and pyloric glands: A comparative study

The Anatomical Record, 2004

Mucins of the gastroduodenal junction are secreted by the mucous surface and mucusproducing gland... more Mucins of the gastroduodenal junction are secreted by the mucous surface and mucusproducing glandular cells in the stomach, and by goblet cells and Brunner's glands in the duodenum. Developmental studies have demonstrated that Brunner's glands can arise from undifferentiated gastric epithelium and/or intestinal epithelium in the proximal duodenum. The aim of this study was to investigate the carbohydrate composition of mucins from this region and compare it with that of mucins from Brunner's glands to evaluate the probable evolution of mucins from these glands. Toward that end, paraffin sections from 13 mammalian species were stained by classic carbohydrate histochemistry and treated with 13 lectins. In general, the mucous surface cells of the stomach, pyloric glands, duodenal goblet cells, and Brunner's glands secretory epithelium had different lectin-binding patterns. However, the lectin-binding profile of the secretory epithelium of Brunner's glands resembled that of pyloric glands more closely than that of duodenal goblet cells and mucous surface cells of the stomach. Mucins from Brunner's glands and pyloric glands showed a greater terminal carbohydrate residue diversity than those of gastric mucous surface cells or duodenal goblet cells. The lectin-binding profile argues for the evolution of similar mucins from the epithelia of Brunner's glands and pyloric glands. The greater diversity of carbohydrate residues in mucins secreted by Brunner's glands suggests that their mucus is more adaptable. This may explain why Brunner's glands metaplasia rather than goblet cell metaplasia is seen in the mucosa adjacent to chronic intestinal ulcers. Anat Rec Part A 278A: 540 -550, 2004. The epithelial cells that line the gastrointestinal tract of mammals are protected in part from the somewhat harsh environment of acid, proteolytic enzymes, and abrasives in the lumen by a mucus layer. In the stomach the mucus layer, which is several microns thick, is secreted by both the epithelium lining the gastric mucosa and mucus-producing cells of the gastric glands, while in the duodenum it is secreted by goblet cells and Brunner's glands, which are located in the submucosa. Mucus is a highly hydrated gel that consists of about 95% water, 5% mucins, and minor components, such as electrolytes . Mucins are high-molecularweight glycoproteins that have gel-forming properties which are highly glycosylated and which in turn are responsible for their protective function.

Research paper thumbnail of Semi-quantitativer Nachweis von Antikörper-SPIO-Konjugaten auf Zelloberflächen mittels MR-Bildgebung und FACS-Untersuchungen

RöFo - Fortschritte auf dem Gebiet der Röntgenstrahlen und der bildgebenden Verfahren, 2008

Research paper thumbnail of In vivo Bildgebung der NIS-Induktion in NIS-negativen extrathyreoidalen Tumoren durch Behandlung mit alltrans-Retinolsäure und Dexamethason

RöFo - Fortschritte auf dem Gebiet der Röntgenstrahlen und der bildgebenden Verfahren, 2013

Research paper thumbnail of Untersuchung der Verteilung magnetisch markierter mesenchymaler Stromazellen im E-/P-Selektin-defizienten Mausmodell mit einem 3.0T MR-Tomographen

RöFo - Fortschritte auf dem Gebiet der Röntgenstrahlen und der bildgebenden Verfahren, 2012

Research paper thumbnail of Wheat germ agglutinin uptake by cytotoxic lymphocytes, a quantitative model of receptor-mediated endocytosis

Protoplasma, 1989

The binding and uptake of fluorescence labeled wheat germ agglufinin into cytotoxic T-cells was m... more The binding and uptake of fluorescence labeled wheat germ agglufinin into cytotoxic T-cells was measured by single cell cytophotometric analysis. The intensity of fluorescence in these cells increased continuously over 24 hrs, indicating a permanent turnover of the ligands for WGA. Although the labeling of the cells was intense, no change in the proliferation rate of this interleukin-2 dependent cell line was observed. Therefore no interaction between the interleukin-2 receptor and other receptors regulating the cellular proliferation with the lectin is likely.

Research paper thumbnail of Fetuin in human bone marrow: detection in foetal tissue and patients with mastocytosis

The Histochemical journal, 2001

Fetuin, a foetal protein of unknown function, has been shown to be expressed in both the immune a... more Fetuin, a foetal protein of unknown function, has been shown to be expressed in both the immune and nervous systems, especially during development. Here, we show for the first time, that fetuin is abundantly present in many cells of the foetal human bone marrow, but is restricted to cells of the monocytic lineage in the adult. Fetuin's immunoreactivity increased considerably in adult human bone marrow in some pathological conditions, particularly in mastocytosis and was also increased in bone marrows in some cases of acute leukaemias, especially in acute myeloid leukaemia. This increase in the presence of fetuin in neoplastic bone marrows is not reflected by an increased level of circulating fetuin. This last observation contradicts earlier suggestions that fetuin is specifically reduced in cancer patients. A consistent increase in fetuin immunoreactivity in bone marrow of most cases of mastocytosis, as demonstrated in this paper, could become a useful tool in the diagnosis of t...

Research paper thumbnail of Exploring the visible human's inner organs with the VOXEL-MAN 3D navigator

Studies in health technology and informatics, 2001

Improved rendering and segmentation techniques lead to a new quality of 3D reconstructions of the... more Improved rendering and segmentation techniques lead to a new quality of 3D reconstructions of the Visible Human. Using these we have implemented an interactive atlas of anatomy and radiology of the inner organs.

Research paper thumbnail of Peritonitis in childhood: aspects of pathogenesis and therapy

Pediatric Surgery International, 2000

The pathogenesis of peritonitis due to hollowviscus perforation is currently accepted as being ma... more The pathogenesis of peritonitis due to hollowviscus perforation is currently accepted as being mainly based on the local and systemic release of pro-and anti-in¯ammatory mediators triggered by the presence of bacteria and bacterial products in the abdominal cavity. Therefore, treatment consists in focal restoration, intraoperative debridement and lavage, and postoperative measures such as drainage, continuous peritoneal lavage, or scheduled reoperation aiming at the removal of infectious agents from the peritoneal cavity to prevent persisting peritonitis and sepsis. In order to evaluate the pathophysiologic relevance of the bacterial and endotoxin load of the peritoneal exudate, we examined the peritoneal¯uid of 20 children with perforated appendicitis for qualitative and quantitative analysis of bacteria, antibiotic concentrations, and endotoxin content. The time period ranged in 12-h intervals from intraoperatively to day 5. Eighteen of 20¯uid specimens (90%) showed endotoxin levels above 1.5 endotoxin units EU/ml (standard <0.1 EU/ml). The most common bacterial species isolated was Escherichia coli, mostly in high concentrations. Despite persisting high endotoxin concentrations and bacterial loads in the peritoneal cavity during the 5 postoperative days, the children recovered uneventfully and the systemic signs of infection disap-peared rapidly. In conclusion, neither the bacterial nor the endotoxin load of the peritoneal cavity proved to be associated with the clinical course. Therefore, we hypothesize that during peritonitis compartmentalization of the focus of infection prevents further systemic reactions and ultimately leads to removal of the infectious agents by endogenous mechanisms. At least in peritonitis due to perforated appendicitis in children, adjuvant surgical measures in addition to appendectomy and intraoperative debridement are not necessary.

Research paper thumbnail of Lectin histochemistry of the gastric mucosa in normal and Helicobacter pylori infected guinea-pigs

Journal of Molecular Histology, 2005

Research paper thumbnail of Comparison of the unmasking of lectin receptors by neuraminidase and by enzyme-free buffer alone

Journal of Histochemistry & Cytochemistry, 1989

Research paper thumbnail of Inositol 1,4,5-Trisphosphate 3-Kinase-A Is a New Cell Motility-promoting Protein That Increases the Metastatic Potential of Tumor Cells by Two Functional Activities

Journal of Biological Chemistry, 2010

In growth factor-stimulated cells, the catalytically active domain enhances basal ITPKA-induced m... more In growth factor-stimulated cells, the catalytically active domain enhances basal ITPKA-induced migration by activating storeoperated calcium entry through production of inositol 1,3,4,5tetrakisphosphate and subsequent inhibition of inositol phosphate 5-phosphatase. These two functional activities of ITPKA stimulating tumor cell migration place the enzyme among the potential targets of anti-metastatic therapy. . The abbreviations used are: Ins(1,4,5)P 3 , inositol 1,4,5-trisphosphate; ITPKA, inositol 1,4,5-trisphosphate 3-kinase-A; HPLC, high pressure liquid chromatography; FCS, fetal calf serum; PBS, phosphate-buffered saline; EGF, epidermal growth factor; SOCE, store-operated Ca 2ϩ entry; CaM, calmodulin; GFP, green fluorescent protein; ITPK, 1,4,5-trisphosphate 3-kinase; GST, glutathione S-transferase; 5-PPT, inositol phosphate 5-phosphatase; IPMK, inositol polyphosphate multikinase; ABD, actin binding domain; Ins(1,3,4,5)P 4 , inositol-1,3,4,5-tetrakisphosphate; shRNA, short hairpin RNA; kd, knockdown.

Research paper thumbnail of Morphologische und laborchemische Untersuchungen an Wildkaninchen (Oryctolagus cuniculus (L.)) — eine vorläufige Bilanz zur Klärung der Frage nach den Ursachen des Besatzrückganges

Zeitschrift für Jagdwissenschaft, 1999

In den letzten Jahren hat sich der Kaninchenbesatz in vielen Regionen Deutschlands trotz anschein... more In den letzten Jahren hat sich der Kaninchenbesatz in vielen Regionen Deutschlands trotz anscheinend guter Habitatbedingungen so stark vermindert, daß keine Bejagung mehr lohnend ist. Im Gegensatz dazu gibt es Regionen, in denen dieser Rückgang nicht zu verzeichnen ist; eine Erklärung für diese lokalen und landesweiten Bestandseinbrüche steht aus.

Research paper thumbnail of Different myosins in myoid and entodermal reticular epithelial cells of the thymus

Cell and Tissue Research, 1978

A sensitive immunofluorescence microscopical technique employing specific antibodies against high... more A sensitive immunofluorescence microscopical technique employing specific antibodies against highly purified actin and (smooth muscle type) myosin from chicken gizzard and myosin from human striated muscle was used to localize these contractile proteins in the thymus of rat, guinea-pig, cat and chicken. Myoid cells were seen to react with antibodies to striated, but not to smooth muscle type myosin, whereas reticular epithelial cells contained smooth, but not striated type myosin. Actin immunoreactivity was found in both myoid and reticular epithelial cells.

Research paper thumbnail of cis Interaction of the Cell Adhesion Molecule CEACAM1 with Integrin β3

The American Journal of Pathology, 2001

CEACAM1 is a cell adhesion molecule that has been implicated in a number of physiological process... more CEACAM1 is a cell adhesion molecule that has been implicated in a number of physiological processes (eg, tumor suppressor in epithelial tissues, potent angiogenic factor in microvessel formation, microbial receptor in human granulocytes and epithelial cells). The mechanism of CEACAM1 action is still largely unresolved but recent findings demonstrated that the cytoplasmic CEACAM1 domain is linked indirectly to the actin-based cytoskeleton. We have isolated integrin ␤ 3 as an associated protein using CEACAM1 tail affinity purification. This association depends on phosphorylation of Tyr-488 in the CEACAM1 cytoplasmic domain. Confocal laser scanning microscopy confirmed in vivo colocalization of both molecules in human granulocytes and epithelial cells. Furthermore, the concentrated colocalization at the tumor-stroma interface of invading melanoma masses suggests a functional role of CEACAM1-integrin ␤ 3 interaction in melanoma invasion. Moreover, colocalization of the two adhesion molecules is also found at the apical surface of glandular cells of pregnancy endometrium. Colocalization of CEACAM1 and integrin ␤ 3 at the transitional zone from proliferative to invasive extravillous trophoblast of the maternalfetal interface supports a role for CEACAM1/integrin ␤ 3 complexes in cell invasion. (Am J Pathol 2001, 159:537-546) Supported by grant Br 1741/1-1 from the Deutsche Forschungsgemeinschaft to J. B. and C. W.

Research paper thumbnail of E-/P-selectins and colon carcinoma metastasis: first in vivo evidence for their crucial role in a clinically relevant model of spontaneous metastasis formation in the lung

British journal of cancer, Jan 2, 2010

Interactions of endothelial selectins with tumour cell glycoconjugates have been shown to have a ... more Interactions of endothelial selectins with tumour cell glycoconjugates have been shown to have a major role in tumour cell dissemination in previous experiments. However, experiments validating this observation were limited in value, as 'metastases' in these experiments were artificially induced by i.v. injection rather than developed spontaneously as in true metastases. Endothelial (E) and platelet (P)-selectin-deficient severe combined immunodeficient (scid) mice were generated and human HT 29 colon cancer cells were subcutaneously inoculated in these mice and in wild-type scid mice. Tumour growth, spontaneous metastasis formation in the lung and adherence of HT29 cells to E- and P-selectin under flow were determined. The number of metastases decreased by 84% in E- and P-selectin-deficient scid mice, compared with wild-type scid mice. The remaining 16% metastases in the E- and P-selectin-deficient scid mice grew within the pulmonary artery and not in the alveolar septae as...

Research paper thumbnail of Binding of mistletoe lectins to cutaneous malignant melanoma: implications for prognosis and therapy

Anticancer research

Glycoconjugates, as detected by lectin histochemistry, have been implicated in metastasis formati... more Glycoconjugates, as detected by lectin histochemistry, have been implicated in metastasis formation in many neoplasias. However, no data concerning the three mistletoe lectins (MLs) and the spread of malignant melanoma have been published. The binding status of ML-I, -II and -III was histochemically assessed in 100 malignant melanomas and correlated with metastasis in a 10 year follow-up period. Furthermore, the staining intensity of the three MLs, scored from negative (-) to very intense (+ + +), was evaluated. Kaplan-Meier analsis revealed that very intense binding (+ + +) of ML-I was positively-correlated with metastasis (p=0.044). Since ML-I is specific for galactose, high density galactose expression in malignant melanoma is a predictor of poor prognosis.

Research paper thumbnail of A realistic model of human structure from the visible human data

Methods of information in medicine, 2001

The computer-based 3D models of the human body reported to date suffer from poor spatial resoluti... more The computer-based 3D models of the human body reported to date suffer from poor spatial resolution. The Visible Human project has delivered high resolution cross-sectional images that are suited for generation of high-quality models. Yet none of the 3D models described to date reflect the quality of the original images. We present a method of segmentation and visualization which provides a new quality of realism and detail. Using the example of a 3D model of the inner organs, we demonstrate that such models, especially when combined with a knowledge base, open new possibilities for scientific, educational, and clinical work.

Research paper thumbnail of Structural, histochemical and biochemical observations on horse milk-fat-globule membranes and casein micelles

Histochemistry, 1988

Horse milk fat globules (MFGs) and casein micelles were studied using freeze fracturing, freeze e... more Horse milk fat globules (MFGs) and casein micelles were studied using freeze fracturing, freeze etching and thin-section electron microscopy, as well as lectin histochemistry, gel electrophoresis, and Western blotting. Horse MFGs were found to be relatively small, their average volume-surface diameter being about 2.75 microns. The MFG membrane is composed of three layers: an inner proteinaceous coat occasionally having a paracrystalline substructure, a unit membrane, and a prominent filamentous glycocalyx. The last is rich in glycoconjugates, as revealed by its binding of various lectins. In addition, the glycocalyx binds cationized ferritin, which indicates the presence of negative electric charges. Gel electrophoresis revealed the presence of high-molecular-weight glycoproteins in the MFG membrane of horse milk. Such glycoproteins are also present in human MFG membranes but are absent in the bovine MFGs. The casein micelles in horse milk are relatively large, their average volume-...

Research paper thumbnail of Histochemistry of mucosubstances in Brunner's-gland cells and duodenal goblet cells of two New World monkey species (Saimiri sciureus and Saguinus fuscicollis)

Histochemistry, 1988

Duodenal goblet cells and Brunner's-gland cells obtained from two species of New World monkey... more Duodenal goblet cells and Brunner's-gland cells obtained from two species of New World monkeys (Saimiri sciureus and Saguinus fuscicollis) were studied using conventional histochemical methods and by applying a panel of 17 labelled lectins. The secretions of both goblet and Brunner's-gland cells were found to contain neutral mucosubstances, while those of goblet cells also exhibit acid and sulphated carbohydrate components. Lectin binding studies allowed a more detailed analysis of the mucus glycoproteins. Marked differences between the two examined species were not detected. N-Acetyl-galactosamine, galactose, fucose and N-Acetyl-glucosamine were found to be the predominant sugar residues in Brunner's-glands glycoproteins, with mannose and glucose being only minor components.

Research paper thumbnail of Basic biochemical data on blood from antarctic Weddell seals (Leptonychotes weddelli): ions, lipids, enzymes, serum proteins and thyroid hormones

Comparative biochemistry and physiology. Comparative physiology, 1992

1. Standard laboratory values of blood samples taken from Weddell seals in Antarctica were determ... more 1. Standard laboratory values of blood samples taken from Weddell seals in Antarctica were determined. 2. Numerous blood parameters are similar to those observed in man. 3. Comparatively high cholesterol levels but normal triglyceride levels were observed when compared with humans. 4. In comparison to laboratory findings in humans, T4 levels were decreased although T3 levels were normal. 5. The levels of alkaline phosphatase are considerably higher than those in humans. 6. The data obtained indicate different lipid and thyroid hormone metabolism in Weddell seals when compared with humans.

Research paper thumbnail of Histochemical similarities of mucins produced by Brunner's glands and pyloric glands: A comparative study

The Anatomical Record, 2004

Mucins of the gastroduodenal junction are secreted by the mucous surface and mucusproducing gland... more Mucins of the gastroduodenal junction are secreted by the mucous surface and mucusproducing glandular cells in the stomach, and by goblet cells and Brunner's glands in the duodenum. Developmental studies have demonstrated that Brunner's glands can arise from undifferentiated gastric epithelium and/or intestinal epithelium in the proximal duodenum. The aim of this study was to investigate the carbohydrate composition of mucins from this region and compare it with that of mucins from Brunner's glands to evaluate the probable evolution of mucins from these glands. Toward that end, paraffin sections from 13 mammalian species were stained by classic carbohydrate histochemistry and treated with 13 lectins. In general, the mucous surface cells of the stomach, pyloric glands, duodenal goblet cells, and Brunner's glands secretory epithelium had different lectin-binding patterns. However, the lectin-binding profile of the secretory epithelium of Brunner's glands resembled that of pyloric glands more closely than that of duodenal goblet cells and mucous surface cells of the stomach. Mucins from Brunner's glands and pyloric glands showed a greater terminal carbohydrate residue diversity than those of gastric mucous surface cells or duodenal goblet cells. The lectin-binding profile argues for the evolution of similar mucins from the epithelia of Brunner's glands and pyloric glands. The greater diversity of carbohydrate residues in mucins secreted by Brunner's glands suggests that their mucus is more adaptable. This may explain why Brunner's glands metaplasia rather than goblet cell metaplasia is seen in the mucosa adjacent to chronic intestinal ulcers. Anat Rec Part A 278A: 540 -550, 2004. The epithelial cells that line the gastrointestinal tract of mammals are protected in part from the somewhat harsh environment of acid, proteolytic enzymes, and abrasives in the lumen by a mucus layer. In the stomach the mucus layer, which is several microns thick, is secreted by both the epithelium lining the gastric mucosa and mucus-producing cells of the gastric glands, while in the duodenum it is secreted by goblet cells and Brunner's glands, which are located in the submucosa. Mucus is a highly hydrated gel that consists of about 95% water, 5% mucins, and minor components, such as electrolytes . Mucins are high-molecularweight glycoproteins that have gel-forming properties which are highly glycosylated and which in turn are responsible for their protective function.

Research paper thumbnail of Semi-quantitativer Nachweis von Antikörper-SPIO-Konjugaten auf Zelloberflächen mittels MR-Bildgebung und FACS-Untersuchungen

RöFo - Fortschritte auf dem Gebiet der Röntgenstrahlen und der bildgebenden Verfahren, 2008

Research paper thumbnail of In vivo Bildgebung der NIS-Induktion in NIS-negativen extrathyreoidalen Tumoren durch Behandlung mit alltrans-Retinolsäure und Dexamethason

RöFo - Fortschritte auf dem Gebiet der Röntgenstrahlen und der bildgebenden Verfahren, 2013

Research paper thumbnail of Untersuchung der Verteilung magnetisch markierter mesenchymaler Stromazellen im E-/P-Selektin-defizienten Mausmodell mit einem 3.0T MR-Tomographen

RöFo - Fortschritte auf dem Gebiet der Röntgenstrahlen und der bildgebenden Verfahren, 2012

Research paper thumbnail of Wheat germ agglutinin uptake by cytotoxic lymphocytes, a quantitative model of receptor-mediated endocytosis

Protoplasma, 1989

The binding and uptake of fluorescence labeled wheat germ agglufinin into cytotoxic T-cells was m... more The binding and uptake of fluorescence labeled wheat germ agglufinin into cytotoxic T-cells was measured by single cell cytophotometric analysis. The intensity of fluorescence in these cells increased continuously over 24 hrs, indicating a permanent turnover of the ligands for WGA. Although the labeling of the cells was intense, no change in the proliferation rate of this interleukin-2 dependent cell line was observed. Therefore no interaction between the interleukin-2 receptor and other receptors regulating the cellular proliferation with the lectin is likely.