Ulrich Dobrindt - Academia.edu (original) (raw)

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Papers by Ulrich Dobrindt

HAL (Le Centre pour la Communication Scientifique Directe), Jun 5, 2005

PLOS Pathogens, May 12, 2016

(A) Wild-type or CEAtg female mice were infected with E</... more (A) Wild-type or CEAtg female mice were infected with E. coli AfaE-III or E. coli ΔAfaE-III. 24 h later, bacteria were re-isolated. Each data point in the graph reflects the number of bacteria re-isolated from an individual animal (n = 10). Data were compiled from two independent experiments. The median for each experimental group of animals is indicated by a line; numbers of recovered bacteria were compared by Mann-Whitney U-test and highly significant differences (p<0.001) are indicated by ***. (B, C) Animals were infected as in (A) and genital tracts were excised, fixed and frozen. (B) Cryosections of genital tracts were co-stained with rabbit antibodies against E. coli (green) and a mouse monoclonal antibody against CEA. Cell nuclei were visualized by Hoechst (blue). Numerous E. coli AfaE-III can be detected in close association with the CEA-positive epithelium (arrowhead), whereas non-CEACAM binding E. coli are rarely observed. (C) Cryosections were co-stained with rabbit antibodies against E. coli (green) and a rat monoclonal antibody against murine CD105 (red). Cell nuclei were visualized by Hoechst (blue). Strong local expression of CD105 can be observed on the mucosal surface of CEAtg mice upon association with E. coli AfaE-III (arrowhead). Pictures in B) and C) are representative for three independent biological replicates.</p

bioRxiv (Cold Spring Harbor Laboratory), Jan 4, 2021

International Journal of Medical Microbiology, Dec 1, 2018

Infection and Immunity, Oct 1, 2017

npj biofilms and microbiomes, Feb 12, 2020

Journal of Clinical Microbiology, Dec 23, 2019

Frontiers in Microbiology, Feb 25, 2021

Food Microbiology, Oct 1, 2016

Microorganisms, Aug 23, 2022

The ability of bacteria to successfully colonize a niche and to adapt to varying growth condition... more The ability of bacteria to successfully colonize a niche and to adapt to varying growth conditions relies in large parts on bacterial genome plasticity. The gain and loss of horizontally acquired genetic information as well as point mutations, intragenomic rearrangements, and large gene amplifications contribute to genomic variability. The chromosomal structure and organization can affect the efficient incorporation of foreign DNA into the chromosome and also gene expression. Genome plasticity should not only be perceived as alterations of genome content. Structural constraints affecting the accessibility and expression of genomic loci shape bacterial genomes and are thus involved in bacterial evolution.

Scientific Reports, Feb 13, 2020

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HAL (Le Centre pour la Communication Scientifique Directe), 2008

Brain, 2021

A close interaction between gut immune responses and distant organ-specific autoimmunity includin... more A close interaction between gut immune responses and distant organ-specific autoimmunity including the CNS in multiple sclerosis has been established in recent years. This so-called gut–CNS axis can be shaped by dietary factors, either directly or via indirect modulation of the gut microbiome and its metabolites. Here, we report that dietary supplementation with conjugated linoleic acid, a mixture of linoleic acid isomers, ameliorates CNS autoimmunity in a spontaneous mouse model of multiple sclerosis, accompanied by an attenuation of intestinal barrier dysfunction and inflammation as well as an increase in intestinal myeloid-derived suppressor-like cells. Protective effects of dietary supplementation with conjugated linoleic acid were not abrogated upon microbiota eradication, indicating that the microbiome is dispensable for these conjugated linoleic acid-mediated effects. Instead, we observed a range of direct anti-inflammatory effects of conjugated linoleic acid on murine myeloi...

Nature Biotechnology, 2021

Is the oncogene MYC upregulated or hyperactive? In the majority of human cancers, finding agents ... more Is the oncogene MYC upregulated or hyperactive? In the majority of human cancers, finding agents that target c-MYC has proved difficult. Here we report specific bacterial effector molecules that inhibit cellular MYC (c-MYC) in human cells. We show that uropathogenic Escherichia coli (UPEC) degrade the c-MYC protein and attenuate MYC expression in both human cells and animal tissues. c-MYC protein was rapidly degraded by both cell-free bacterial lysates and the purified bacterial protease Lon. In mice, intravesical or peroral delivery of Lon protease delayed tumor progression and increased survival in MYC-dependent bladder and colon cancer models, respectively. These results suggest that bacteria have evolved strategies to control c-MYC tissue levels in the host and that the Lon protease shows promise for therapeutic targeting of c-MYC in cancer.