Umit Goktolga - Academia.edu (original) (raw)

Papers by Umit Goktolga

Journal of Minimally Invasive Gynecology, 2008

Taiwanese Journal of Obstetrics & Gynecology, Dec 1, 2008

Fertility and Sterility, Sep 1, 2013

The aim of this study is to investigate the efficacy of the ovarian stimulation protocols on blas... more The aim of this study is to investigate the efficacy of the ovarian stimulation protocols on blastocyst quality and on the pregnancy outcome in women who had a history of multiple failure of ICSI with embryo transfer (BT). DESIGN: Retrospective study included 103 infertile couples. MATERIALS AND METHODS: Our study focused on 103 infertile couples, who had a history of 2 or more failures of intracytoplasmic sperm injection (ICSI) with embryo transfer. The protocols were classified in four groups: agonists with short protocol (11.7%), with long protocol (35%), and antagonists (53.4%). Gonadotropins were regrouped in two groups: recombinant FSH (71.8%) and HMG (28.2%).Sperm and oocytes preparation techniques for ICSI and evaluation of embryo quality were interpreted according to WHO criteria. RESULTS: Comparison of the results showed that with the antagonist protocol, the blastocyst rate (67.75%) was higher than with the agonist protocols (62.47% with the long and 61.55% with the short protocol) without a statistically significant difference. For the pregnancy outcome after BT, the long protocol was associated with a significantly higher pregnancy rate (63.9%) versus 50% with the antagonist and 30.9% with the short protocol (p¼0.002). In addition, for Gonadotropins,the blastocyst rate was higher with FSH-rec (4.3% vs. 2.5%with HMG) and the pregnancy rate after BT was significantly higher with the FSH-rec 53.7% versus 24.1% with the HMG (p¼0.017). There was no significant difference in the oocyte retrieval between all groups with a mean number of 9.6 oocytes. CONCLUSION: We found that the antagonist protocol with FSH-rec gonadotropin seems to be very useful for improving blastocyst rate. However, the long protocol showed better results in term of pregnancy rate after BT in patients with a history of multiple failure of conception after embryo transferbut this could be due to the fact that the long protocol is usually prescribed in younger patients.

Turkiye Klinikleri Journal of Gynecology and Obstetrics Special Topics, 2013

Fertility and Sterility, Sep 1, 2013

Sperm sample were obtained at 0h, 4h and 18h post-insemination to evaluate sperm functionality. C... more Sperm sample were obtained at 0h, 4h and 18h post-insemination to evaluate sperm functionality. Cleavage rate and blastocyst yield were evaluated at 48 h and d7 post-insemination respectively. Statistical were based in One way ANOVA for cleavage and embryo development, Two way ANOVA and Fisher Multiple comparison test for sperm functionality. RESULTS: High concentrations of melatonin (10-3M) triggered significantly lower blastocyst percentages when compared to other treatments. Lower concentrations of melatonin did not have any effect on embryo development and total cell number compared to control. Sperm motility decreased through incubation time (18 hours) and 10-8M melatonin show significantly less wobbler index than samples with 10-6M melatonin. Viability, plasma membrane integrity, acrosome and viable sperm with intact mitochondrial membrane potential decreased significantly through incubation time, but were not affected by melatonin concentration. Intracellular hydrogen peroxide (H2O2) increased significantly through incubation time, and 10-6M melatonin showed less percentage vs. 10-8M melatonin. DNA fragmentation increased through incubation time and DNA oxidation was not influenced by time. Samples with 10-6M melatonin showed more DNA fragmentation and more DNA oxidation than controls. CONCLUSION: Melatonin modulates fertilization in cattle, and it has a dual effect as an antioxidant or a pro-oxidant depending on concentration and cellular structures. Despite melatonin effects on functional sperm parameters, they were not reflected on in vitro embryo production.

Ginekologia Polska, Sep 29, 2017

Objectives: The aim of this study was to evaluate the relationship between in vitro fertilization... more Objectives: The aim of this study was to evaluate the relationship between in vitro fertilization (IVF) cycle outcomes, serum and follicular fluid (FF) levels of leptin and ghrelin. Material and methods: Forty-four women who underwent intracytoplasmic sperm injection cycles (ICSI) were enrolled in the study. On the third day (D3) of the menstrual cycle, venous blood samples were drawn for serum measurements of leptin and ghrelin. The follicular fluid (FF) and the corresponding oocyte were obtained from a single dominant preovulatory follicle at the time of oocyte pickup. The FF and D3 serum leptin and ghrelin concentrations were measured by enzyme-linked immunosorbent assay. The relationship between pregnancy rate and serum, follicular fluid levels of leptin and ghrelin were analyzed. Results: Of the 44 cases included, nineteen achieved clinical pregnancy (43.18%). Follicular fluid ghrelin levels were significantly lower in the pregnant group than non-pregnant group (p < 0.05) With respect to FF leptin, there was no statistically significant differences between the pregnant and non-pregnant women (p > 0.05). There was no statistically significant difference in D3 serum ghrelin between pregnant and non-pregnant groups (p > 0.05). However, D3 serum leptin levels were significantly lower in pregnant women than non-pregnant women (p < 0.05). Conclusions: Lower ghrelin levels in the follicular fluid were associated with higher pregnancy rates. Also, D3 serum leptin levels were inversely correlated with clinical pregnancy rates. These findings support the potential role of these molecules on IVF outcomes.

Journal of Obstetrics and Gynaecology Research, Jul 6, 2011

To investigate the coexistence of endometriosis and uterine septum patients whose complaints are... more To investigate the coexistence of endometriosis and uterine septum patients whose complaints are abortion and infertility. Ninety-two patients with a uterine septum and 191 patients who had undergone diagnostic laparoscopy for infertility were reviewed. The incidence of endometriosis in patients with a uterine septum was compared with patients with a normal uterine cavity; then the incidence of endometriosis was compared in association with having a preoperative diagnosis of presumed unexplained infertility in women with a partial or complete uterine septum. There was no significant difference between the patients who had a uterine septum and those with a normal cavity in terms of the incidence of endometriosis (P = 0.39). Also, the incidence of endometriosis was not significantly different in patients who had a complete or partial uterine septum (P = 0.49). Endometriosis was observed in 8.7% of the patients who were presumed to have unexplained infertility in the complete uterine septum group and in 18.8% of the patients in the partial uterine septum group; but the difference was not statistically significant when complete and partial uterine septum groups were compared according to the type of infertility (partial uterine septum group, P = 0.13; complete uterine septum group, P = 0.28). An increased incidence of endometriosis was not observed in patients with a septate uterus. The reason for infertility in women with a partial uterine septum may be related to endometriosis.

Journal of Minimally Invasive Gynecology, 2008

We evaluated effectiveness of tension-free vaginal tape application for surgical relief of intrin... more We evaluated effectiveness of tension-free vaginal tape application for surgical relief of intrinsic sphincter deficiency. Design: A prospective study (Canadian Task Force classification II-3). Setting: Tertiary center of medical faculty. Patients: We studied 47 patients. Interventions: Tension-free vaginal tape procedure, questionnaire form, stress test, cotton swab test, and functional bladder volume measurements. Measurements and Main Results: Patients were grouped as intrinsic sphincter deficiency according to American College of Obstetricians and Gynecologists criteria. Operative results were documented at 6, 12, 36, and 60 months after the procedure by using a questionnaire form and objective tests of stress test, cotton swab test, and mean bladder functional volume measurement. At first visit 6 months after procedure, 70% (n ϭ 35) of patients were completely satisfied, 9 (18%) had improved urine control, and 5 (10%) had no change in urine control. Results were: 72% (n ϭ 36), 12% (n ϭ 6), and 14 (n ϭ 7%) at the end of the first year, and 66% (n ϭ 33), 20% (n ϭ 10), and 14% (n ϭ 7) at the end of the third year, respectively. The fifth year's follow-up visit revealed 57.4% (n ϭ 27 of 47) satisfaction, 17.02% (8 of 47) improved urine control, and 25.5% (12 of 47) no change in urine control. Conclusion: Tension-free vaginal tape procedure is a safe and effective technique for patients who have exclusively intrinsic sphincter deficiency. Long-term results will clarify the value of this procedure in comparison with classic antistress surgical techniques.

gulhanemedicaljournal.org

OBJECTIVE: Our purpose was to investigate the diagnostic accuracy of transvaginal ultrasonography... more OBJECTIVE: Our purpose was to investigate the diagnostic accuracy of transvaginal ultrasonography (TVUS), color doppler ultrasonography (CDUS) and CA-125 assay in postmenauposal women undergoing laparotomy-laparoscopy for a clinical diagnosis of ...

International Journal of Gynecological Cancer, 2007

modern tıp kitapevi, 2017

Türk Fertilite Dergisi, 2005

Gülhane tıp dergisi, 2014

International Journal of Gynecologic Cancer, 2004

TURKISH JOURNAL OF MEDICAL SCIENCES, 2020

Background/aim: To evaluate the effects of hysterectomy on rat ovaries and the possible protectiv... more Background/aim: To evaluate the effects of hysterectomy on rat ovaries and the possible protective role of peroxisome proliferatoractivated receptor gamma (PPAR-γ) agonist-rosiglitazone against ovarian reserve decrement. Materials and methods: Forty-five adult Wistar albino rats were randomly divided into three groups. Hysterectomy was performed (n = 15) in group 1 [H]; 1 mg/kg/day PPAR-γ agonist/rosiglitazone was used for 50 days after hysterectomy (n = 15) in group 2 [H + R]; a sham operation was performed (n = 15) in group 3 [control, C]. Blood samples were collected for anti-Müllerian hormone (AMH) evaluation in all groups and simultaneous ovarian Doppler examination was performed in [H] and [H + R] groups before and after (50 days) hysterectomy. All animals were sacrificed to obtain ovaries for histological examination. Results: AMH levels were found to be significantly decreased at postoperative day 50 in all groups (P < 0.05). Histopathologic analysis showed that primary, preantral, and antral follicle counts were significantly higher in the [H] group as compared to the [C] and [H + R] groups (P < 0.05). There was no significant difference between the [C] and [H + R] groups in terms of follicle numbers (P > 0.05). In the ovarian Doppler blood flow analysis, all parameters were significantly decreased in group [H] (P < 0.05), but not in the [H + R] group (P > 0.05) on postoperative day 50. Conclusion: Hysterectomy affects the histopathological structure of rat ovaries and PPAR-γ agonist-rosiglitazone improves the ovarian Doppler blood flow parameters.

International Journal of Gynecologic Cancer, 2003

International Journal of Gynecologic Cancer, 2003

Türk Üreme Tıbbı ve Cerrahisi Dergisi, 2017

The aim of our study was to compare the Paraoxonase (PON1) and arylesterase (ARES) activity in fo... more The aim of our study was to compare the Paraoxonase (PON1) and arylesterase (ARES) activity in follicle fluids of infertile females with polycystic ovary syndrome (PCOS). M Ma at te er ri ia al l a an nd d M Me et th ho od ds s: : A total of 93 women who underwent assisted reproductive technologies (ART) using intra-cytoplasmic sperm injection (ICSI) were enrolled in this prospective study, of whom 46, with PCOS, comprised the study group, the remaining 47 women with MFI formed the control group. Women age, body mass index, (BMI) basal hormone levels, total follicle count, total oxidant status (TOS), total antioxidant status (TAS), PON1 and ARES activity, oxidative stress index (OSI), simulated paraoxonase (SPON) were compared between groups. R Re es su ul lt ts s: : There were no significant differences for average age, BMI, Day3-FSH, Day3-LH, Day3-E2 and total follicle count among groups. Also there were no statistically significant differences among groups in terms of TOS, TAS, PON1, SPON, ARES and OSI (p>0.05). C Co on nc cl lu us si io on n: : TOS, TAS, OSI, SPON, PON1 and ARES enzyme activities in follicular fluid of infertile females with PCOS are similar in those with male factor infertility.

Gulhane Medical Journal, 2004

Journal of Minimally Invasive Gynecology, 2008

Taiwanese Journal of Obstetrics & Gynecology, Dec 1, 2008

Fertility and Sterility, Sep 1, 2013

The aim of this study is to investigate the efficacy of the ovarian stimulation protocols on blas... more The aim of this study is to investigate the efficacy of the ovarian stimulation protocols on blastocyst quality and on the pregnancy outcome in women who had a history of multiple failure of ICSI with embryo transfer (BT). DESIGN: Retrospective study included 103 infertile couples. MATERIALS AND METHODS: Our study focused on 103 infertile couples, who had a history of 2 or more failures of intracytoplasmic sperm injection (ICSI) with embryo transfer. The protocols were classified in four groups: agonists with short protocol (11.7%), with long protocol (35%), and antagonists (53.4%). Gonadotropins were regrouped in two groups: recombinant FSH (71.8%) and HMG (28.2%).Sperm and oocytes preparation techniques for ICSI and evaluation of embryo quality were interpreted according to WHO criteria. RESULTS: Comparison of the results showed that with the antagonist protocol, the blastocyst rate (67.75%) was higher than with the agonist protocols (62.47% with the long and 61.55% with the short protocol) without a statistically significant difference. For the pregnancy outcome after BT, the long protocol was associated with a significantly higher pregnancy rate (63.9%) versus 50% with the antagonist and 30.9% with the short protocol (p¼0.002). In addition, for Gonadotropins,the blastocyst rate was higher with FSH-rec (4.3% vs. 2.5%with HMG) and the pregnancy rate after BT was significantly higher with the FSH-rec 53.7% versus 24.1% with the HMG (p¼0.017). There was no significant difference in the oocyte retrieval between all groups with a mean number of 9.6 oocytes. CONCLUSION: We found that the antagonist protocol with FSH-rec gonadotropin seems to be very useful for improving blastocyst rate. However, the long protocol showed better results in term of pregnancy rate after BT in patients with a history of multiple failure of conception after embryo transferbut this could be due to the fact that the long protocol is usually prescribed in younger patients.

Turkiye Klinikleri Journal of Gynecology and Obstetrics Special Topics, 2013

Fertility and Sterility, Sep 1, 2013

Sperm sample were obtained at 0h, 4h and 18h post-insemination to evaluate sperm functionality. C... more Sperm sample were obtained at 0h, 4h and 18h post-insemination to evaluate sperm functionality. Cleavage rate and blastocyst yield were evaluated at 48 h and d7 post-insemination respectively. Statistical were based in One way ANOVA for cleavage and embryo development, Two way ANOVA and Fisher Multiple comparison test for sperm functionality. RESULTS: High concentrations of melatonin (10-3M) triggered significantly lower blastocyst percentages when compared to other treatments. Lower concentrations of melatonin did not have any effect on embryo development and total cell number compared to control. Sperm motility decreased through incubation time (18 hours) and 10-8M melatonin show significantly less wobbler index than samples with 10-6M melatonin. Viability, plasma membrane integrity, acrosome and viable sperm with intact mitochondrial membrane potential decreased significantly through incubation time, but were not affected by melatonin concentration. Intracellular hydrogen peroxide (H2O2) increased significantly through incubation time, and 10-6M melatonin showed less percentage vs. 10-8M melatonin. DNA fragmentation increased through incubation time and DNA oxidation was not influenced by time. Samples with 10-6M melatonin showed more DNA fragmentation and more DNA oxidation than controls. CONCLUSION: Melatonin modulates fertilization in cattle, and it has a dual effect as an antioxidant or a pro-oxidant depending on concentration and cellular structures. Despite melatonin effects on functional sperm parameters, they were not reflected on in vitro embryo production.

Ginekologia Polska, Sep 29, 2017

Objectives: The aim of this study was to evaluate the relationship between in vitro fertilization... more Objectives: The aim of this study was to evaluate the relationship between in vitro fertilization (IVF) cycle outcomes, serum and follicular fluid (FF) levels of leptin and ghrelin. Material and methods: Forty-four women who underwent intracytoplasmic sperm injection cycles (ICSI) were enrolled in the study. On the third day (D3) of the menstrual cycle, venous blood samples were drawn for serum measurements of leptin and ghrelin. The follicular fluid (FF) and the corresponding oocyte were obtained from a single dominant preovulatory follicle at the time of oocyte pickup. The FF and D3 serum leptin and ghrelin concentrations were measured by enzyme-linked immunosorbent assay. The relationship between pregnancy rate and serum, follicular fluid levels of leptin and ghrelin were analyzed. Results: Of the 44 cases included, nineteen achieved clinical pregnancy (43.18%). Follicular fluid ghrelin levels were significantly lower in the pregnant group than non-pregnant group (p < 0.05) With respect to FF leptin, there was no statistically significant differences between the pregnant and non-pregnant women (p > 0.05). There was no statistically significant difference in D3 serum ghrelin between pregnant and non-pregnant groups (p > 0.05). However, D3 serum leptin levels were significantly lower in pregnant women than non-pregnant women (p < 0.05). Conclusions: Lower ghrelin levels in the follicular fluid were associated with higher pregnancy rates. Also, D3 serum leptin levels were inversely correlated with clinical pregnancy rates. These findings support the potential role of these molecules on IVF outcomes.

Journal of Obstetrics and Gynaecology Research, Jul 6, 2011

To investigate the coexistence of endometriosis and uterine septum patients whose complaints are... more To investigate the coexistence of endometriosis and uterine septum patients whose complaints are abortion and infertility. Ninety-two patients with a uterine septum and 191 patients who had undergone diagnostic laparoscopy for infertility were reviewed. The incidence of endometriosis in patients with a uterine septum was compared with patients with a normal uterine cavity; then the incidence of endometriosis was compared in association with having a preoperative diagnosis of presumed unexplained infertility in women with a partial or complete uterine septum. There was no significant difference between the patients who had a uterine septum and those with a normal cavity in terms of the incidence of endometriosis (P = 0.39). Also, the incidence of endometriosis was not significantly different in patients who had a complete or partial uterine septum (P = 0.49). Endometriosis was observed in 8.7% of the patients who were presumed to have unexplained infertility in the complete uterine septum group and in 18.8% of the patients in the partial uterine septum group; but the difference was not statistically significant when complete and partial uterine septum groups were compared according to the type of infertility (partial uterine septum group, P = 0.13; complete uterine septum group, P = 0.28). An increased incidence of endometriosis was not observed in patients with a septate uterus. The reason for infertility in women with a partial uterine septum may be related to endometriosis.

Journal of Minimally Invasive Gynecology, 2008

We evaluated effectiveness of tension-free vaginal tape application for surgical relief of intrin... more We evaluated effectiveness of tension-free vaginal tape application for surgical relief of intrinsic sphincter deficiency. Design: A prospective study (Canadian Task Force classification II-3). Setting: Tertiary center of medical faculty. Patients: We studied 47 patients. Interventions: Tension-free vaginal tape procedure, questionnaire form, stress test, cotton swab test, and functional bladder volume measurements. Measurements and Main Results: Patients were grouped as intrinsic sphincter deficiency according to American College of Obstetricians and Gynecologists criteria. Operative results were documented at 6, 12, 36, and 60 months after the procedure by using a questionnaire form and objective tests of stress test, cotton swab test, and mean bladder functional volume measurement. At first visit 6 months after procedure, 70% (n ϭ 35) of patients were completely satisfied, 9 (18%) had improved urine control, and 5 (10%) had no change in urine control. Results were: 72% (n ϭ 36), 12% (n ϭ 6), and 14 (n ϭ 7%) at the end of the first year, and 66% (n ϭ 33), 20% (n ϭ 10), and 14% (n ϭ 7) at the end of the third year, respectively. The fifth year's follow-up visit revealed 57.4% (n ϭ 27 of 47) satisfaction, 17.02% (8 of 47) improved urine control, and 25.5% (12 of 47) no change in urine control. Conclusion: Tension-free vaginal tape procedure is a safe and effective technique for patients who have exclusively intrinsic sphincter deficiency. Long-term results will clarify the value of this procedure in comparison with classic antistress surgical techniques.

gulhanemedicaljournal.org

OBJECTIVE: Our purpose was to investigate the diagnostic accuracy of transvaginal ultrasonography... more OBJECTIVE: Our purpose was to investigate the diagnostic accuracy of transvaginal ultrasonography (TVUS), color doppler ultrasonography (CDUS) and CA-125 assay in postmenauposal women undergoing laparotomy-laparoscopy for a clinical diagnosis of ...

International Journal of Gynecological Cancer, 2007

modern tıp kitapevi, 2017

Türk Fertilite Dergisi, 2005

Gülhane tıp dergisi, 2014

International Journal of Gynecologic Cancer, 2004

TURKISH JOURNAL OF MEDICAL SCIENCES, 2020

Background/aim: To evaluate the effects of hysterectomy on rat ovaries and the possible protectiv... more Background/aim: To evaluate the effects of hysterectomy on rat ovaries and the possible protective role of peroxisome proliferatoractivated receptor gamma (PPAR-γ) agonist-rosiglitazone against ovarian reserve decrement. Materials and methods: Forty-five adult Wistar albino rats were randomly divided into three groups. Hysterectomy was performed (n = 15) in group 1 [H]; 1 mg/kg/day PPAR-γ agonist/rosiglitazone was used for 50 days after hysterectomy (n = 15) in group 2 [H + R]; a sham operation was performed (n = 15) in group 3 [control, C]. Blood samples were collected for anti-Müllerian hormone (AMH) evaluation in all groups and simultaneous ovarian Doppler examination was performed in [H] and [H + R] groups before and after (50 days) hysterectomy. All animals were sacrificed to obtain ovaries for histological examination. Results: AMH levels were found to be significantly decreased at postoperative day 50 in all groups (P < 0.05). Histopathologic analysis showed that primary, preantral, and antral follicle counts were significantly higher in the [H] group as compared to the [C] and [H + R] groups (P < 0.05). There was no significant difference between the [C] and [H + R] groups in terms of follicle numbers (P > 0.05). In the ovarian Doppler blood flow analysis, all parameters were significantly decreased in group [H] (P < 0.05), but not in the [H + R] group (P > 0.05) on postoperative day 50. Conclusion: Hysterectomy affects the histopathological structure of rat ovaries and PPAR-γ agonist-rosiglitazone improves the ovarian Doppler blood flow parameters.

International Journal of Gynecologic Cancer, 2003

International Journal of Gynecologic Cancer, 2003

Türk Üreme Tıbbı ve Cerrahisi Dergisi, 2017

The aim of our study was to compare the Paraoxonase (PON1) and arylesterase (ARES) activity in fo... more The aim of our study was to compare the Paraoxonase (PON1) and arylesterase (ARES) activity in follicle fluids of infertile females with polycystic ovary syndrome (PCOS). M Ma at te er ri ia al l a an nd d M Me et th ho od ds s: : A total of 93 women who underwent assisted reproductive technologies (ART) using intra-cytoplasmic sperm injection (ICSI) were enrolled in this prospective study, of whom 46, with PCOS, comprised the study group, the remaining 47 women with MFI formed the control group. Women age, body mass index, (BMI) basal hormone levels, total follicle count, total oxidant status (TOS), total antioxidant status (TAS), PON1 and ARES activity, oxidative stress index (OSI), simulated paraoxonase (SPON) were compared between groups. R Re es su ul lt ts s: : There were no significant differences for average age, BMI, Day3-FSH, Day3-LH, Day3-E2 and total follicle count among groups. Also there were no statistically significant differences among groups in terms of TOS, TAS, PON1, SPON, ARES and OSI (p>0.05). C Co on nc cl lu us si io on n: : TOS, TAS, OSI, SPON, PON1 and ARES enzyme activities in follicular fluid of infertile females with PCOS are similar in those with male factor infertility.

Gulhane Medical Journal, 2004